FastQCFastQC Report
Thu 26 May 2016
SRR2132717_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132717_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2367205
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT873153.6885271871257452No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA299181.2638533629322344No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT69470.29346845752691464No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG43670.18447916424644253No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41780.1764950648549661No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24480.10341309688007587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT18350.041.9298321
AACGAGT223350.040.8449441
ACGAGTG253650.039.0753482
CGAGTGG265700.038.2199063
GAGTACT147400.037.96550420
GAGTGGA277700.037.384324
GTGGAAT284200.036.9232986
GTACTTT148750.036.91111422
AGTACTT150300.036.88171421
TGGAATC286350.036.6898427
AGTGGAA282600.036.6808475
GAATCAC287100.036.623879
GGAATCA287200.036.604238
TGAGTAC283200.036.4835319
AATCACT288600.036.36491810
GCTGAGT289800.036.24469817
CTGAGTA286650.036.19790318
ATCACTG291400.036.12872711
TCACTGC294550.035.83944312
CTGCTGA295100.035.72046715
Produced by FastQC (version 0.11.5)