##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2132716_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3616262 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.883743489824575 33.0 31.0 34.0 25.0 34.0 2 31.61307394209822 34.0 31.0 34.0 28.0 34.0 3 31.81518761638399 34.0 31.0 34.0 28.0 34.0 4 35.74838687019912 37.0 35.0 37.0 35.0 37.0 5 35.401704577820965 37.0 35.0 37.0 33.0 37.0 6 35.741977489462876 37.0 35.0 37.0 35.0 37.0 7 35.92674922336933 37.0 35.0 37.0 35.0 37.0 8 36.076394077641496 37.0 37.0 37.0 35.0 37.0 9 37.993021799858525 39.0 39.0 39.0 37.0 39.0 10-11 38.068286672812974 39.0 39.0 39.0 37.0 39.0 12-13 38.050833153128835 39.0 39.0 39.0 37.0 39.0 14-15 39.59102935572699 41.0 40.0 41.0 37.5 41.0 16-17 39.51292923466275 41.0 40.0 41.0 37.0 41.0 18-19 39.42197205290988 41.0 40.0 41.0 36.5 41.0 20-21 39.28917249358592 41.0 39.5 41.0 36.0 41.0 22-23 39.17245030918667 41.0 39.0 41.0 36.0 41.0 24-25 39.00323123711722 41.0 39.0 41.0 35.0 41.0 26-27 38.95962737213178 41.0 39.0 41.0 35.0 41.0 28-29 38.806804927297854 41.0 39.0 41.0 35.0 41.0 30-31 38.658771128861794 41.0 39.0 41.0 35.0 41.0 32-33 38.506220788206164 41.0 39.0 41.0 35.0 41.0 34-35 38.38686024408629 41.0 38.0 41.0 35.0 41.0 36-37 38.166097893349544 40.5 38.0 41.0 34.5 41.0 38-39 37.770248809405956 40.0 36.5 41.0 34.0 41.0 40-41 37.61344850013633 40.0 36.0 41.0 33.5 41.0 42-43 37.51529300144735 40.0 36.0 41.0 33.0 41.0 44-45 37.342709267193584 40.0 35.0 41.0 33.0 41.0 46-47 37.23582375945106 40.0 35.0 41.0 33.0 41.0 48-49 37.0654204534959 40.0 35.0 41.0 33.0 41.0 50-51 36.797683768488014 40.0 35.0 41.0 32.5 41.0 52-53 36.286389094595464 39.5 35.0 41.0 30.5 41.0 54-55 35.34543915789287 39.0 35.0 41.0 27.0 41.0 56-57 34.808774502511156 39.0 35.0 41.0 22.5 41.0 58-59 34.589169285853735 39.0 35.0 41.0 20.0 41.0 60-61 34.329994480488416 39.0 35.0 41.0 18.0 41.0 62-63 34.09487241245242 38.0 35.0 40.5 17.5 41.0 64-65 33.7386169752081 37.5 34.5 40.0 14.0 41.0 66-67 33.29605778010553 37.0 34.0 40.0 11.5 41.0 68-69 33.04672048098285 36.0 34.0 39.0 10.5 41.0 70-71 32.77068157672204 36.0 34.0 39.0 8.0 41.0 72-73 32.43598527982762 35.5 34.0 39.0 7.0 40.5 74-75 32.08101846602929 35.0 33.0 38.0 2.0 40.0 76-77 31.70459925193473 35.0 33.0 37.0 2.0 39.5 78-79 31.363636954402086 35.0 33.0 37.0 2.0 39.0 80-81 31.018560878608906 35.0 33.0 36.0 2.0 39.0 82-83 30.584914616252917 35.0 32.0 36.0 2.0 37.5 84-85 30.23586205866721 35.0 31.0 35.5 2.0 37.0 86-87 30.194605921805447 35.0 31.5 35.0 2.0 37.0 88-89 30.28942275200193 35.0 33.0 35.0 2.0 36.5 90-91 30.135796300157455 35.0 33.0 35.0 2.0 36.0 92-93 30.01521045764936 35.0 33.0 35.0 2.0 36.0 94-95 29.78594955232779 35.0 32.0 35.0 2.0 36.0 96-97 29.495926456656072 35.0 31.5 35.0 2.0 35.0 98-99 29.386425679334074 35.0 32.0 35.0 2.0 35.0 100 29.373810028145083 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.900726533682132 1101 2 1.4030157618456371 1101 3 1.3160692612269251 1101 4 0.6893389972283828 1101 5 1.0108918504487718 1101 6 0.671555437163228 1101 7 0.5049903611975424 1101 8 0.4026909173244704 1101 9 0.41992878300305847 1101 10-11 0.27782565135189685 1101 12-13 0.3465824531517754 1101 14-15 0.4180215699129164 1101 16-17 0.4081345750434551 1101 18-19 0.40048854091885744 1101 20-21 0.5121498816036691 1101 22-23 0.49478094717285614 1101 24-25 0.4743287455946046 1101 26-27 0.5154234582758193 1101 28-29 0.5873218394035291 1101 30-31 0.6118639577085005 1101 32-33 0.5769947127678208 1101 34-35 0.5313792688126853 1101 36-37 0.5582612660269604 1101 38-39 0.5837394696911886 1101 40-41 0.5955155376433297 1101 42-43 0.6331582827620466 1101 44-45 0.6558699118369802 1101 46-47 0.7265395699312691 1101 48-49 0.5947805801562112 1101 50-51 0.5506436372168508 1101 52-53 0.5955346508421542 1101 54-55 0.47525241070119506 1101 56-57 0.5100989387320283 1101 58-59 0.5180987780123516 1101 60-61 0.47011583132511703 1101 62-63 0.5538602785013751 1101 64-65 0.5235066509734736 1101 66-67 0.5256988119861035 1101 68-69 0.5517310666536517 1101 70-71 0.5866970492411241 1101 72-73 0.5527394036105449 1101 74-75 0.523596563132557 1101 76-77 0.5336866621729186 1101 78-79 0.5382571073167952 1101 80-81 0.5794354983454753 1101 82-83 0.5330471665497498 1101 84-85 0.5239454381429809 1101 86-87 0.6226944045668183 1101 88-89 0.6224459541858884 1101 90-91 0.6303107271636499 1101 92-93 0.6619523627374555 1101 94-95 0.5942276815851351 1101 96-97 0.5345745308333285 1101 98-99 0.5202237228517639 1101 100 0.5171521343003143 1102 1 1.268868827772657 1102 2 1.1246009769827623 1102 3 1.1193303898582876 1102 4 0.5936239792699993 1102 5 0.8945621891596502 1102 6 0.606958164187489 1102 7 0.32121345927894396 1102 8 0.30259742145310753 1102 9 0.3225301951956183 1102 10-11 0.2680525260775468 1102 12-13 0.2598447872909304 1102 14-15 0.31712470892636446 1102 16-17 0.35553279475691113 1102 18-19 0.33751686299005 1102 20-21 0.39058707418767824 1102 22-23 0.38450735211856824 1102 24-25 0.408562314109318 1102 26-27 0.38442456163753036 1102 28-29 0.3593764203791423 1102 30-31 0.3640005132138455 1102 32-33 0.3564132439071912 1102 34-35 0.3720187308801357 1102 36-37 0.4671888334134593 1102 38-39 0.3583534541336917 1102 40-41 0.31371221761965984 1102 42-43 0.33039155035993417 1102 44-45 0.34948527571167887 1102 46-47 0.2504889146779661 1102 48-49 0.31941821463704656 1102 50-51 0.33839811669055564 1102 52-53 0.3479967394626229 1102 54-55 0.29097651137858094 1102 56-57 0.29252203699747525 1102 58-59 0.20538499592951354 1102 60-61 0.21111156235515693 1102 62-63 0.3138353953482067 1102 64-65 0.29058046025845385 1102 66-67 0.24863309156636149 1102 68-69 0.21511188104318535 1102 70-71 0.22030895303240072 1102 72-73 0.23388213297220517 1102 74-75 0.235871531177402 1102 76-77 0.24003876799257995 1102 78-79 0.32106440367570244 1102 80-81 0.32097278764618054 1102 82-83 0.31355594605133064 1102 84-85 0.3694511026492435 1102 86-87 0.31600977184639234 1102 88-89 0.32488683139066055 1102 90-91 0.3729479272935876 1102 92-93 0.40458611198631544 1102 94-95 0.40487277549051015 1102 96-97 0.4502658251345899 1102 98-99 0.45854290450522583 1102 100 0.47519356143748936 1103 1 1.8648273646528963 1103 2 1.322987151858598 1103 3 1.2498112477321222 1103 4 0.6793851298277431 1103 5 0.9292877719221408 1103 6 0.5847899224254149 1103 7 0.4322555672965649 1103 8 0.34439398827628764 1103 9 0.33761458100942576 1103 10-11 0.2303814806880169 1103 12-13 0.25870982082464167 1103 14-15 0.35168373109306117 1103 16-17 0.37600011433785596 1103 18-19 0.39501403687751946 1103 20-21 0.4388678136401438 1103 22-23 0.4844298600966894 1103 24-25 0.4464349104472518 1103 26-27 0.4783128020439875 1103 28-29 0.5226231161330119 1103 30-31 0.48471800363244455 1103 32-33 0.4920528365950432 1103 34-35 0.453079565050146 1103 36-37 0.5238041200671404 1103 38-39 0.4991953668244946 1103 40-41 0.4637539860801496 1103 42-43 0.5439724182379919 1103 44-45 0.5000090474810577 1103 46-47 0.4999203112647379 1103 48-49 0.5415229605182859 1103 50-51 0.5767819713679216 1103 52-53 0.48507362503936235 1103 54-55 0.28273345234028113 1103 56-57 0.37523332414387056 1103 58-59 0.4193700655175405 1103 60-61 0.4292793029282933 1103 62-63 0.37369451987084545 1103 64-65 0.3274368292195504 1103 66-67 0.33499828396197984 1103 68-69 0.42040317185887943 1103 70-71 0.3945325989831474 1103 72-73 0.46766441004496073 1103 74-75 0.40605817028844626 1103 76-77 0.4094352117018545 1103 78-79 0.38333431868539947 1103 80-81 0.5066097244888788 1103 82-83 0.4491904062776868 1103 84-85 0.3880581086058328 1103 86-87 0.3957733967393864 1103 88-89 0.3982581384216779 1103 90-91 0.38397465422023913 1103 92-93 0.48952553916450725 1103 94-95 0.515897938238421 1103 96-97 0.500226101511938 1103 98-99 0.5964724304319766 1103 100 0.6365706155219009 1104 1 1.863980184834631 1104 2 1.2904427706153996 1104 3 1.191407569578967 1104 4 0.6420759046193751 1104 5 0.9540461299784084 1104 6 0.590010672454234 1104 7 0.3495261726475434 1104 8 0.23579024321520592 1104 9 0.21292239580240135 1104 10-11 0.16693366723070113 1104 12-13 0.19774229666357002 1104 14-15 0.2820759268258115 1104 16-17 0.2936470376913647 1104 18-19 0.29344375719831106 1104 20-21 0.3265452279599117 1104 22-23 0.28924364944870007 1104 24-25 0.33351702718391607 1104 26-27 0.23408742320012266 1104 28-29 0.2522130501337685 1104 30-31 0.2665374903244455 1104 32-33 0.24502445655988225 1104 34-35 0.2727966147326413 1104 36-37 0.25644508614381323 1104 38-39 0.22224037046157719 1104 40-41 0.2634661435699712 1104 42-43 0.28585381040463176 1104 44-45 0.2701701441955535 1104 46-47 0.27229605183838856 1104 48-49 0.2741067623869853 1104 50-51 0.41490661937012163 1104 52-53 0.3790396496370221 1104 54-55 0.24966825902437506 1104 56-57 0.34348632886199226 1104 58-59 0.30486962908327797 1104 60-61 0.27637662018307907 1104 62-63 0.2824209333790506 1104 64-65 0.29031892033788154 1104 66-67 0.2860007572719283 1104 68-69 0.28031121490204924 1104 70-71 0.2891303037189914 1104 72-73 0.3027931960789374 1104 74-75 0.351295562843589 1104 76-77 0.3550523239600025 1104 78-79 0.3251826205174204 1104 80-81 0.24854313921278504 1104 82-83 0.3289769507458864 1104 84-85 0.3481753405881953 1104 86-87 0.28377262365407674 1104 88-89 0.2423782248608397 1104 90-91 0.23261822029088108 1104 92-93 0.22053343486320998 1104 94-95 0.25263177558646177 1104 96-97 0.31123446562870427 1104 98-99 0.25842504404056044 1104 100 0.28301065386493107 1105 1 1.5776382158944458 1105 2 1.2637371080516644 1105 3 1.1777429534906751 1105 4 0.6428272343935078 1105 5 1.0239861418087273 1105 6 0.6842714526156684 1105 7 0.37279686005631874 1105 8 0.33037747104854986 1105 9 0.24761284111551873 1105 10-11 0.24679166779781525 1105 12-13 0.25745327377722305 1105 14-15 0.31640900741731315 1105 16-17 0.32276663605464506 1105 18-19 0.30632108728676855 1105 20-21 0.36334782684104994 1105 22-23 0.4039120125621736 1105 24-25 0.40766863454465607 1105 26-27 0.42779728881552614 1105 28-29 0.46745137436299444 1105 30-31 0.5053265361712391 1105 32-33 0.49997987993327087 1105 34-35 0.49661390876030254 1105 36-37 0.5111038232944907 1105 38-39 0.4526017354553886 1105 40-41 0.437381822772835 1105 42-43 0.46262912726271566 1105 44-45 0.48987045038042965 1105 46-47 0.4948492325738485 1105 48-49 0.4290001205408487 1105 50-51 0.43840994242252407 1105 52-53 0.3870398909112467 1105 54-55 0.3211420747839213 1105 56-57 0.24475792135223884 1105 58-59 0.19177739139967542 1105 60-61 0.2421042559790223 1105 62-63 0.3060164392524598 1105 64-65 0.2083900836570507 1105 66-67 0.25830410096600787 1105 68-69 0.2772959690076817 1105 70-71 0.2656193082369711 1105 72-73 0.2913945042035593 1105 74-75 0.3230180183666107 1105 76-77 0.34285067917296175 1105 78-79 0.24987779962489043 1105 80-81 0.2954985978982876 1105 82-83 0.2495738021171725 1105 84-85 0.3010285068805487 1105 86-87 0.30537174439743353 1105 88-89 0.33330204752367365 1105 90-91 0.32712215016616497 1105 92-93 0.394260542795994 1105 94-95 0.3563026864770116 1105 96-97 0.4246791804121024 1105 98-99 0.3992676071488539 1105 100 0.4563759641167664 1106 1 1.6693817116965874 1106 2 1.3204135519707378 1106 3 1.17201202739275 1106 4 0.660154410138091 1106 5 0.9728636789690199 1106 6 0.6611546505481272 1106 7 0.4113122840085026 1106 8 0.3026329326658015 1106 9 0.2609245396775677 1106 10-11 0.19135617013078132 1106 12-13 0.18336507292627147 1106 14-15 0.28090825668585495 1106 16-17 0.34746898663338754 1106 18-19 0.31917302248524493 1106 20-21 0.38225111647654586 1106 22-23 0.38164346362145807 1106 24-25 0.3696994457384477 1106 26-27 0.31555790643440673 1106 28-29 0.42916190406002386 1106 30-31 0.42575590317063217 1106 32-33 0.4229810987246836 1106 34-35 0.4370189917078733 1106 36-37 0.42578592798573567 1106 38-39 0.46659740727744037 1106 40-41 0.42462144377276445 1106 42-43 0.4391804982194998 1106 44-45 0.41864483079753256 1106 46-47 0.47826546452601093 1106 48-49 0.5497772101991103 1106 50-51 0.5225427466339667 1106 52-53 0.5728476522630785 1106 54-55 0.5862698459741864 1106 56-57 0.630884367290399 1106 58-59 0.5336387832447826 1106 60-61 0.637277529939098 1106 62-63 0.6683743968223581 1106 64-65 0.6847973105027663 1106 66-67 0.5523060870086454 1106 68-69 0.5728030339597225 1106 70-71 0.6345729647947707 1106 72-73 0.5490419430896125 1106 74-75 0.5370806319399861 1106 76-77 0.5540864859431238 1106 78-79 0.562749099656827 1106 80-81 0.5388423080902456 1106 82-83 0.590972089935498 1106 84-85 0.554980438040861 1106 86-87 0.5416826977346076 1106 88-89 0.5799460392858187 1106 90-91 0.5426530953535895 1106 92-93 0.5923685159679017 1106 94-95 0.508666756359279 1106 96-97 0.531069483678241 1106 98-99 0.4920510954620383 1106 100 0.5414221276771087 1107 1 1.5944702973439178 1107 2 1.2253658010187678 1107 3 1.1853340109716548 1107 4 0.6499897282248455 1107 5 0.9678063865482898 1107 6 0.621032477884178 1107 7 0.4153029902341814 1107 8 0.36772629776914556 1107 9 0.36890982453488874 1107 10-11 0.2648337127926581 1107 12-13 0.2093732503313106 1107 14-15 0.31432263726661347 1107 16-17 0.2965052634022598 1107 18-19 0.28934318158530914 1107 20-21 0.35157180576481295 1107 22-23 0.3402836386123411 1107 24-25 0.3324347598132533 1107 26-27 0.295774953044571 1107 28-29 0.35588940703845395 1107 30-31 0.37873789997153295 1107 32-33 0.3568188449146348 1107 34-35 0.3149663897824837 1107 36-37 0.2818308234743796 1107 38-39 0.3315965787221131 1107 40-41 0.3666858547920242 1107 42-43 0.29926961588657264 1107 44-45 0.3354629515642742 1107 46-47 0.3800035468477816 1107 48-49 0.4072995332240481 1107 50-51 0.3862710014173416 1107 52-53 0.3211222686419646 1107 54-55 0.3905121539281424 1107 56-57 0.5434355676490341 1107 58-59 0.508261668466325 1107 60-61 0.49450411976234676 1107 62-63 0.5920355996753841 1107 64-65 0.6386133572646884 1107 66-67 0.5868259348335556 1107 68-69 0.5715666724165516 1107 70-71 0.5611173657667052 1107 72-73 0.5764173414502949 1107 74-75 0.5892308812915275 1107 76-77 0.6537967706696222 1107 78-79 0.6691356739206853 1107 80-81 0.6730484910317713 1107 82-83 0.6032520300004194 1107 84-85 0.6263310530033479 1107 86-87 0.6204655237885746 1107 88-89 0.5658530282808591 1107 90-91 0.5175812029157676 1107 92-93 0.5644440732367713 1107 94-95 0.5672534677272303 1107 96-97 0.5526984409021125 1107 98-99 0.5832889396831682 1107 100 0.5568229304163985 1108 1 0.8203047287648424 1108 2 0.9262580469083233 1108 3 0.9881606077626834 1108 4 0.5180370751656724 1108 5 0.8760820519642465 1108 6 0.5438958548520958 1108 7 0.3623152917462136 1108 8 0.297652398253895 1108 9 0.26454230414437774 1108 10-11 0.19478933288658595 1108 12-13 0.13009959601723864 1108 14-15 0.15395736036639818 1108 16-17 0.2007863604263136 1108 18-19 0.19084566688517413 1108 20-21 0.19465843890892387 1108 22-23 0.19367449959256788 1108 24-25 0.17099264525455737 1108 26-27 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-0.8339177083378431 2215 70-71 -0.8572938133165096 2215 72-73 -0.8477822089278924 2215 74-75 -0.8438228927531561 2215 76-77 -0.8383678022852763 2215 78-79 -0.8453655971381053 2215 80-81 -0.9396192857882397 2215 82-83 -0.960559736837233 2215 84-85 -0.9415261997501823 2215 86-87 -0.8987707259894222 2215 88-89 -0.8095315163133527 2215 90-91 -0.8294585080447376 2215 92-93 -0.8673401548786757 2215 94-95 -0.9205732663707167 2215 96-97 -1.0110658421318455 2215 98-99 -0.9330667116373341 2215 100 -0.9044531499236292 2216 1 -6.087765974890765 2216 2 -6.136876186600862 2216 3 -5.560205709285825 2216 4 -3.071249598516154 2216 5 -4.0420821189675 2216 6 -4.375807381254933 2216 7 -2.246764867174747 2216 8 -1.5090554512498429 2216 9 -1.17740981226018 2216 10-11 -1.076934631758327 2216 12-13 -0.9048452152546247 2216 14-15 -1.1927851777995429 2216 16-17 -1.1447986227259221 2216 18-19 -1.1020228065369224 2216 20-21 -1.1440135041391457 2216 22-23 -1.1460936746452361 2216 24-25 -1.6505552983079923 2216 26-27 -1.5480529871818476 2216 28-29 -2.30758422379882 2216 30-31 -1.7285359281243657 2216 32-33 -1.4645064349891115 2216 34-35 -1.4230557645618234 2216 36-37 -1.4819856192655791 2216 38-39 -1.3941955969826125 2216 40-41 -1.4140001470738142 2216 42-43 -1.4786145686202872 2216 44-45 -1.5094341214692335 2216 46-47 -1.570908079290426 2216 48-49 -1.6419445126013272 2216 50-51 -1.564109672130762 2216 52-53 -1.5593058181384833 2216 54-55 -1.3404383234844062 2216 56-57 -1.343932430865415 2216 58-59 -1.3544570645418048 2216 60-61 -1.440595126674154 2216 62-63 -1.4957617198757873 2216 64-65 -1.5829033991542758 2216 66-67 -1.556844276335461 2216 68-69 -1.5048057149353227 2216 70-71 -1.4608101422476203 2216 72-73 -1.521019964446225 2216 74-75 -1.6330158654424167 2216 76-77 -1.6123614231797738 2216 78-79 -1.539206786608137 2216 80-81 -1.5380426915970133 2216 82-83 -1.5666347297654895 2216 84-85 -1.6710563266705591 2216 86-87 -1.5586503595330683 2216 88-89 -1.494676678933537 2216 90-91 -1.572627086171515 2216 92-93 -1.6522907740434754 2216 94-95 -1.7704751893685717 2216 96-97 -1.7852398173732418 2216 98-99 -1.8985460880653555 2216 100 -1.8979538081892464 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5367.0 3 3283.0 4 4014.0 5 5341.0 6 4088.0 7 3405.0 8 3292.0 9 3721.0 10 4306.0 11 10919.0 12 7308.0 13 10510.0 14 9060.0 15 9419.0 16 10803.0 17 13654.0 18 17546.0 19 26670.0 20 56273.0 21 84433.0 22 38577.0 23 21915.0 24 21295.0 25 23063.0 26 24030.0 27 25556.0 28 29259.0 29 34050.0 30 40028.0 31 49073.0 32 63668.0 33 86833.0 34 128968.0 35 275512.0 36 402776.0 37 745568.0 38 939893.0 39 368908.0 40 3878.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 13.908139574456783 44.842573247371384 24.421379591423396 16.827907586748445 2 17.135401140735933 42.46423516880137 23.19204194828804 17.208321742174654 3 18.132462820704266 23.294785220089263 19.174423299209682 39.39832865999679 4 36.64686531801434 24.421694063356203 21.2440117558179 17.68742886281155 5 16.9371766698397 41.536514148952705 22.294757080284057 19.231552100923537 6 39.3832617148666 21.511589802754475 22.491056102991216 16.614092379387706 7 18.54658207840029 21.95844770096857 41.67037675920605 17.824593461425085 8 38.462672228948016 22.537083872794614 21.603136055960547 17.397107842296826 9 38.23884995058433 23.902637585440438 22.0047662475783 15.85374621639693 10-11 16.2410937039407 44.467049124206156 22.096864109956634 17.19499306189651 12-13 16.125117593802663 23.094372033884714 32.448699790004156 28.33181058230847 14-15 16.608236133463855 33.5495926730932 21.66927986372661 28.172891329716336 16-17 27.658178877921742 22.52762112395866 32.37399806540138 17.44020193271822 18-19 17.077648135007916 23.164499696095028 32.26363300004259 27.494219168854467 20-21 27.90425583102109 33.648861725173674 21.98661214259365 16.460270301211583 22-23 27.12165331032614 23.073672800075215 32.605633997555486 17.19903989204316 24-25 16.54553607390867 33.53477749815169 21.998134197570142 27.921552230369496 26-27 17.046732476617585 28.917412894873184 36.941735779283206 17.09411884922602 28-29 29.672538577841134 22.70408872229416 30.748646206578538 16.874726493286165 30-31 24.752064950106025 26.18244457792276 31.383425311692147 17.682065160279066 32-33 19.004915414768366 27.27398425131527 32.24498261284368 21.47611772107268 34-35 20.86134749845693 27.20005498763124 32.56067136910489 19.37792614480694 36-37 21.33822764089793 25.12853158464456 34.637662980123814 18.895577794333697 38-39 23.907183401125273 25.332570501129315 32.39188001351966 18.368366084225748 40-41 18.510867733176926 30.290265726062525 32.44437199242904 18.75449454833151 42-43 18.5398390018769 25.31677372128337 34.90352916580362 21.239858111036106 44-45 18.544474058095762 27.829646606586678 31.984126161068303 21.64175317424926 46-47 21.37611240013235 25.10465683329584 34.33234975658999 19.18688100998182 48-49 18.97826246875655 25.482555383663197 34.26421158652838 21.27497056105187 50-51 21.41043445050389 27.878677592562145 31.92202958266263 18.788858374271335 52-53 21.310272261313344 25.712509972498715 33.699330373500324 19.277887392687617 54-55 19.32497980434622 28.497816712238826 30.093819562999514 22.083383920415432 56-57 19.89947566951693 29.21506675811108 30.902672536808222 19.982785035563765 58-59 25.03067404918053 27.253310190726005 27.9186186673282 19.797397092765266 60-61 22.26639776935995 29.870430122766784 27.84122798587667 20.021944121996597 62-63 19.8323527988441 29.672337866144943 27.52924665915423 22.96606267585673 64-65 21.804624710525193 29.59201043305445 28.15299895694346 20.450365899476896 66-67 22.220304865284014 27.08391579963878 30.646010095437365 20.049769239639843 68-69 24.42220682543486 27.31719767838815 28.675421081471143 19.585174414705847 70-71 19.147591361448647 31.86353198402579 28.963674449906506 20.02520220461906 72-73 19.014474101025712 27.31983630337773 31.200406406994098 22.465283188602463 74-75 19.28806249485789 29.58686828112177 28.39359481633943 22.73147440768091 76-77 22.02092914566055 27.089692782929255 30.39784182012771 20.49153625128249 78-79 19.9752527925307 27.54985360621822 30.0006928001197 22.47420080113138 80-81 22.466350357282664 29.902547708785004 27.75524274300054 19.875859190931795 82-83 22.147271246416814 27.88179526500721 29.739051314423048 20.23188217415293 84-85 20.022555125725606 30.160262796528624 27.275261443525277 22.54192063422049 86-87 20.016496846862108 30.315147852817155 29.384700799329615 20.283654500991126 88-89 24.818114106379845 27.92338790532447 27.23451020870447 20.02398777959122 90-91 22.166678259772883 30.41092560143675 27.1735587567894 20.24883738200096 92-93 19.87573620009431 30.377133580127747 26.855486981064498 22.891643238713442 94-95 21.879198268619504 30.71163643221903 26.679376583554433 20.729788715607036 96-97 22.291775559814486 28.41092056538705 28.802866759035712 20.49443711576275 98-99 24.55614571566028 28.58950259916135 26.653313735389872 20.201037949788496 100 19.74595910022825 32.815980896735006 26.770540946650417 20.667519056386325 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 83447.0 1 44633.5 2 4246.5 3 2259.5 4 1833.5 5 1710.0 6 1732.0 7 2117.5 8 2019.5 9 1868.5 10 2010.5 11 2940.0 12 27632.5 13 31447.0 14 10547.5 15 10123.5 16 11436.5 17 12756.5 18 13865.0 19 14926.5 20 16392.0 21 18207.5 22 20259.0 23 23142.5 24 26022.0 25 28689.5 26 32218.0 27 35814.5 28 39245.5 29 43062.5 30 47335.5 31 51345.5 32 55458.0 33 60286.0 34 64882.0 35 68559.0 36 72470.0 37 76623.5 38 80280.5 39 82892.0 40 85012.0 41 87599.0 42 89474.0 43 90738.0 44 92422.0 45 94502.5 46 99027.0 47 109991.0 48 118076.5 49 154368.0 50 302915.5 51 301017.0 52 140989.5 53 89056.0 54 78928.0 55 74264.5 56 68945.0 57 62070.0 58 55379.5 59 47615.5 60 39641.5 61 32669.0 62 26334.0 63 20508.0 64 16012.5 65 12464.0 66 9658.5 67 7360.0 68 5684.5 69 4414.0 70 3407.5 71 2565.0 72 1981.5 73 1596.5 74 1249.5 75 970.5 76 766.0 77 569.5 78 395.0 79 246.0 80 133.0 81 90.0 82 57.0 83 34.0 84 18.5 85 9.5 86 7.0 87 4.0 88 4.0 89 2.5 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05820927797819959 2 0.0 3 5.5305727295201514E-5 4 2.7652863647600754E-4 5 0.010397476731497884 6 0.003014162137588482 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 5.5305727295201514E-5 16-17 2.2122290918080606E-4 18-19 0.0 20-21 0.0 22-23 5.5305727295201514E-5 24-25 0.0034151286604786934 26-27 0.006733472298190784 28-29 9.540237958422261E-4 30-31 0.0053646555476345465 32-33 0.0024057991373412657 34-35 0.02498436230560728 36-37 0.025039668032902484 38-39 0.021790456554309397 40-41 0.0020877912053938567 42-43 0.005392308411282147 44-45 0.006899389480076389 46-47 0.001064635250432629 48-49 0.00840647054887063 50-51 0.009028659980941645 52-53 0.0018527418643892505 54-55 0.01064635250432629 56-57 0.006138935729767367 58-59 0.011628029163816118 60-61 0.007175918116552396 62-63 0.001023155954961228 64-65 0.012347003618653736 66-67 0.0047839454110349304 68-69 0.001866568296213051 70-71 0.008530908435284833 72-73 0.005046647615687138 74-75 0.007867239707742415 76-77 0.006968521639195389 78-79 0.013840258255624177 80-81 0.007950198298685216 82-83 0.011531244141049515 84-85 0.0064292907980671755 86-87 0.011600376300168518 88-89 0.018555071507540107 90-91 0.01679911466591746 92-93 0.015679173688189628 94-95 0.005198738365748942 96-97 0.00918075073100345 98-99 0.015941875892841836 100 0.015070810687942412 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3616262.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.91265259826671 #Duplication Level Percentage of deduplicated Percentage of total 1 84.07170610085484 45.325286843589666 2 11.16910805318819 12.043124846080758 3 2.3703691952522368 3.833786728598005 4 0.7785802381214205 1.6790130359086366 5 0.36841485169057636 0.9931110955617998 6 0.23508674806226 0.7604490107242122 7 0.1695904870868271 0.6400151109998062 8 0.1290175637004255 0.5564543272684628 9 0.09181768806081171 0.44551216069187055 >10 0.5462367060366269 6.239836722297838 >50 0.04550185300751679 1.5698705619323172 >100 0.018626691619589776 2.0311888176722945 >500 0.0017573042856916298 0.6561088430485709 >1k 0.0022224730669095395 3.348618363537907 >5k 0.0017573042854633568 6.484292542157764 >10k+ 2.0674168064274784E-4 13.393330989930005 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 248195 6.86330249301627 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG 138870 3.8401531747423165 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 80888 2.2367848347271297 No Hit GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT 12698 0.35113606259723434 No Hit TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG 9350 0.25855427510506707 No Hit AGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTC 9250 0.255788988740307 No Hit CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA 8645 0.2390590062335085 No Hit TACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACT 8255 0.22827438941094422 No Hit TCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGA 8022 0.22183127218105325 No Hit TCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGT 8017 0.22169300786281523 No Hit CCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATT 7979 0.22064219904420643 No Hit TCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCA 7894 0.21829170563416037 No Hit CTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCG 7866 0.21751742545202754 No Hit CCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGAT 7837 0.21671549240624713 No Hit ACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTC 7576 0.2094980949942233 No Hit CCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTC 7427 0.2053778183107308 No Hit TCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACT 7389 0.20432700949212196 No Hit CGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAG 7370 0.20380160508281756 No Hit ACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGA 7358 0.20346977071904634 No Hit TGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCA 7257 0.2006768314906387 No Hit ATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTA 7232 0.19998550989944866 No Hit AACGAGTGGAATCACTGCTGAGTACATGGTAACGAGTGGAATCACTGCTG 6873 0.19005813184995998 No Hit CAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTT 6691 0.18502531066609665 No Hit ACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAG 6455 0.17849923484526287 No Hit CTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGC 6264 0.17321753788857114 No Hit TGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATG 6262 0.1731622321612759 No Hit GATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGT 6192 0.17122653170594387 No Hit GTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCAC 6076 0.16801879952282217 No Hit GAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGT 6003 0.16600014047654732 No Hit GTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCAT 5820 0.1609396664290364 No Hit ATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCC 5435 0.1502933139247101 No Hit ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 5277 0.14592416146838919 No Hit TTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTAC 5192 0.14357366805834312 No Hit CTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACG 5114 0.14141674469383025 No Hit TGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTG 5103 0.14111256319370666 No Hit CGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAG 5090 0.14075307596628783 No Hit TGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGA 5069 0.14017236582968823 No Hit CTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGT 5064 0.1400341015114502 No Hit CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 4919 0.1360244362825481 No Hit TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 4845 0.13397812437262566 No Hit ATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGG 4730 0.13079804505315157 No Hit ACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATC 4517 0.1249079850962126 No Hit ATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCAC 4508 0.12465910932338421 No Hit GCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCC 4281 0.11838190927537884 No Hit CAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCC 4170 0.11531244141049514 No Hit TGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACA 4128 0.11415102113729592 No Hit ACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAAC 4011 0.11091563609052663 No Hit TACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAAT 3893 0.10765259818010973 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0027929392284076764 2.7652863647600754E-5 0.0 0.0 0.0 2 0.0027929392284076764 2.7652863647600754E-5 0.0 0.0 0.0 3 0.0027929392284076764 2.7652863647600754E-5 0.0 0.0 0.0 4 0.0027929392284076764 2.7652863647600754E-5 0.0 0.0 0.0 5 0.0027929392284076764 2.7652863647600754E-5 0.0 0.0 0.0 6 0.0027929392284076764 2.7652863647600754E-5 0.0 0.0 0.0 7 0.0027929392284076764 2.7652863647600754E-5 0.0 0.0 2.7652863647600754E-5 8 0.0027929392284076764 2.7652863647600754E-5 0.0 0.0 2.7652863647600754E-5 9 0.0027929392284076764 2.7652863647600754E-5 0.0 0.0 2.7652863647600754E-5 10-11 0.002820592092055277 2.7652863647600754E-5 0.0 0.0 8.295859094280226E-5 12-13 0.002889724251174279 5.530572729520151E-5 0.0 0.0 8.295859094280226E-5 14-15 0.0031386000240026858 5.530572729520151E-5 0.0 0.0 8.295859094280226E-5 16-17 0.0035395665468928965 5.530572729520151E-5 0.0 0.0 9.678502276660264E-5 18-19 0.0037469630242499023 9.678502276660264E-5 0.0 0.0 1.6591718188560452E-4 20-21 0.00400966522890211 1.1061145459040302E-4 0.0 0.0 1.797436137094049E-4 22-23 0.004465937479087522 1.1061145459040302E-4 0.0 0.0 1.9357004553320528E-4 24-25 0.005074300479334739 1.244378864142034E-4 0.0 0.0 1.9357004553320528E-4 26-27 0.005765622070524758 1.6591718188560452E-4 0.0 0.0 2.0739647735700564E-4 28-29 0.006346332207124373 1.9357004553320528E-4 0.0 0.0 2.488757728284068E-4 30-31 0.0069132159119001885 1.9357004553320528E-4 0.0 1.3826431823800377E-5 2.7652863647600754E-4 32-33 0.007728975389504411 2.2122290918080603E-4 0.0 2.7652863647600754E-5 3.8714009106641055E-4 34-35 0.009001007117294046 2.488757728284068E-4 0.0 2.7652863647600754E-5 3.8714009106641055E-4 36-37 0.00991355161766487 2.7652863647600754E-4 0.0 2.7652863647600754E-5 4.147929547140113E-4 38-39 0.01136532695916391 2.7652863647600754E-4 0.0 2.7652863647600754E-5 4.5627225018541245E-4 40-41 0.012734143709720147 3.0418150012360826E-4 0.0 2.7652863647600754E-5 5.254044093044143E-4 42-43 0.014365662664928593 3.8714009106641055E-4 0.0 2.7652863647600754E-5 5.530572729520151E-4 44-45 0.016287536688436844 3.8714009106641055E-4 0.0 2.7652863647600754E-5 5.807101365996159E-4 46-47 0.017891402779997685 4.1479295471401133E-4 0.0 2.7652863647600754E-5 6.360158638948174E-4 48-49 0.019426136712439528 4.286193865378117E-4 0.0 2.7652863647600754E-5 6.774951593662185E-4 50-51 0.021638365804247592 4.8392511383301323E-4 0.0 2.7652863647600754E-5 6.913215911900189E-4 52-53 0.024555742919069472 4.977515456568136E-4 0.0 2.7652863647600754E-5 7.3280088666142E-4 54-55 0.027639037215776954 5.254044093044143E-4 0.0 2.7652863647600754E-5 7.466273184852203E-4 56-57 0.030888248694370044 5.530572729520151E-4 0.0 2.7652863647600754E-5 8.019330457804219E-4 58-59 0.03384710510466332 5.530572729520151E-4 0.0 2.7652863647600754E-5 8.572387730756234E-4 60-61 0.037704679583503625 5.945365684234163E-4 0.0 2.7652863647600754E-5 8.848916367232241E-4 62-63 0.04293107081290017 6.083630002472166E-4 0.0 2.7652863647600754E-5 8.848916367232241E-4 64-65 0.049346535179143544 6.360158638948173E-4 0.0 2.7652863647600754E-5 8.848916367232241E-4 66-67 0.055831131704505925 6.636687275424181E-4 0.0 2.7652863647600754E-5 8.987180685470246E-4 68-69 0.0627305211845823 6.636687275424181E-4 0.0 2.7652863647600754E-5 9.401973640184257E-4 70-71 0.07145499966540035 6.636687275424181E-4 0.0 2.7652863647600754E-5 9.540237958422261E-4 72-73 0.08154829489677462 6.913215911900189E-4 0.0 2.7652863647600754E-5 9.955030913136272E-4 74-75 0.09334224124247634 6.913215911900189E-4 0.0 2.7652863647600754E-5 9.955030913136272E-4 76-77 0.1061316906794917 6.913215911900189E-4 0.0 2.7652863647600754E-5 9.955030913136272E-4 78-79 0.12129928639020071 7.051480230138192E-4 0.0 2.7652863647600754E-5 9.955030913136272E-4 80-81 0.14047654732981185 7.604537503090208E-4 0.0 2.7652863647600754E-5 9.955030913136272E-4 82-83 0.1650322902488813 7.881066139566214E-4 0.0 2.7652863647600754E-5 9.955030913136272E-4 84-85 0.1947314658064045 8.019330457804219E-4 0.0 2.7652863647600754E-5 9.955030913136272E-4 86-87 0.22979529691156228 8.019330457804219E-4 0.0 2.7652863647600754E-5 0.001023155954961228 88 0.2587754980142479 8.295859094280227E-4 0.0 2.7652863647600754E-5 0.0010508088186088286 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGAGT 1765 0.0 75.953575 1 CGTGTGG 1850 0.0 52.84418 3 ACGTGTG 1700 0.0 52.53035 2 AACGTGT 1595 0.0 51.01918 1 TCGAGTG 980 0.0 45.56204 2 ATCGAGT 935 0.0 44.77406 1 GTGGAAT 162670 0.0 44.18568 6 TGGAATC 164290 0.0 43.8826 7 AGTGGAA 162785 0.0 43.583725 5 GGAATCA 165580 0.0 43.54356 8 GAATCAC 165765 0.0 43.46378 9 AACGAGT 168670 0.0 40.746487 1 ACGAGTG 172305 0.0 40.70378 2 CGAGTGG 174840 0.0 40.36631 3 GAGTGGA 177520 0.0 39.96607 4 AGTACTT 37550 0.0 39.28583 20-21 GAGTACT 37000 0.0 39.133125 20-21 GTACTTT 36765 0.0 38.42536 22-23 TACTTTT 38265 0.0 37.64984 22-23 CGATTGG 570 0.0 37.105923 3 >>END_MODULE