##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2132715_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2836324 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.948265078319686 33.0 31.0 34.0 25.0 34.0 2 31.678294510782266 34.0 31.0 34.0 28.0 34.0 3 31.87409759956902 34.0 31.0 34.0 28.0 34.0 4 35.81807332307592 37.0 35.0 37.0 35.0 37.0 5 35.4690127079981 37.0 35.0 37.0 33.0 37.0 6 35.82585346384969 37.0 36.0 37.0 35.0 37.0 7 36.00854063217037 37.0 35.0 37.0 35.0 37.0 8 36.156181028683605 37.0 37.0 37.0 35.0 37.0 9 38.08459717578105 39.0 39.0 39.0 37.0 39.0 10-11 38.1546112150798 39.0 39.0 39.0 37.0 39.0 12-13 38.13703476753713 39.0 39.0 39.0 37.0 39.0 14-15 39.68135251825955 41.0 40.0 41.0 38.0 41.0 16-17 39.61642428721119 41.0 40.0 41.0 37.5 41.0 18-19 39.56397770494485 41.0 40.0 41.0 37.0 41.0 20-21 39.44718727479653 41.0 40.0 41.0 37.0 41.0 22-23 39.34850461371832 41.0 39.5 41.0 37.0 41.0 24-25 39.21843643391939 41.0 39.0 41.0 36.0 41.0 26-27 39.17819244204823 41.0 39.0 41.0 36.0 41.0 28-29 39.05306269664538 41.0 39.0 41.0 35.5 41.0 30-31 38.931219776019944 41.0 39.0 41.0 35.0 41.0 32-33 38.78683993789144 41.0 39.0 41.0 35.0 41.0 34-35 38.6705119020253 41.0 39.0 41.0 35.0 41.0 36-37 38.463916851530364 40.5 38.5 41.0 35.0 41.0 38-39 38.09399948665949 40.0 37.0 41.0 35.0 41.0 40-41 37.93262564502504 40.0 37.0 41.0 34.0 41.0 42-43 37.83182069467381 40.0 37.0 41.0 34.0 41.0 44-45 37.66564891740154 40.0 36.5 41.0 33.5 41.0 46-47 37.55703015593423 40.0 36.0 41.0 33.0 41.0 48-49 37.39046596933214 40.0 36.0 41.0 33.0 41.0 50-51 37.13306078572124 40.0 35.0 41.0 33.0 41.0 52-53 36.65448781591948 40.0 35.0 41.0 32.0 41.0 54-55 35.78680097901368 39.0 35.0 41.0 29.0 41.0 56-57 35.233431194743616 39.0 35.0 41.0 25.5 41.0 58-59 34.98632578647573 39.0 35.0 41.0 24.0 41.0 60-61 34.718740172138304 39.0 35.0 41.0 23.0 41.0 62-63 34.46378604842042 38.0 35.0 40.5 22.5 41.0 64-65 34.11204414587333 37.5 35.0 40.0 22.0 41.0 66-67 33.68393966979795 37.0 34.5 40.0 21.0 41.0 68-69 33.43098531761534 36.0 34.0 39.0 20.5 41.0 70-71 33.14760901786961 36.0 34.0 39.0 20.0 41.0 72-73 32.81095618836212 35.5 34.0 39.0 19.5 40.5 74-75 32.45240829326974 35.0 34.0 37.5 18.0 40.0 76-77 32.07927320715123 35.0 33.0 37.0 14.0 39.0 78-79 31.72712867077245 35.0 33.0 36.5 11.0 39.0 80-81 31.389857435187235 35.0 33.0 36.0 7.0 39.0 82-83 30.986151617375164 35.0 32.5 36.0 4.0 37.0 84-85 30.656062389205182 35.0 32.0 35.0 2.0 37.0 86-87 30.602932351875175 35.0 32.5 35.0 2.0 37.0 88-89 30.717702208915483 35.0 33.0 35.0 2.0 36.0 90-91 30.578407826468343 35.0 33.0 35.0 2.0 36.0 92-93 30.4504363746878 35.0 33.0 35.0 2.0 36.0 94-95 30.248398631468056 35.0 33.0 35.0 2.0 36.0 96-97 30.00783179213658 35.0 32.5 35.0 2.0 35.0 98-99 29.908727634783617 35.0 33.0 35.0 2.0 35.0 100 29.876295867467892 35.0 33.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.8556212272656012 1101 2 1.371515616103192 1101 3 1.2663496140572974 1101 4 0.639420886950191 1101 5 0.9232156866601784 1101 6 0.6030287802238803 1101 7 0.3871903203778473 1101 8 0.2697879656391251 1101 9 0.304296321881651 1101 10-11 0.14826751466374333 1101 12-13 0.21998129077563533 1101 14-15 0.2672404371137489 1101 16-17 0.2882901134856297 1101 18-19 0.2523387577146252 1101 20-21 0.37405585533229413 1101 22-23 0.3313471764011524 1101 24-25 0.3339732508440605 1101 26-27 0.3468281459277307 1101 28-29 0.4235531182570682 1101 30-31 0.4400235005397306 1101 32-33 0.37950720417580897 1101 34-35 0.3661943459837502 1101 36-37 0.43310515460834864 1101 38-39 0.48343208005996985 1101 40-41 0.4145484165370803 1101 42-43 0.4560111694813287 1101 44-45 0.44638353299114897 1101 46-47 0.4303940678267537 1101 48-49 0.3775817459037256 1101 50-51 0.33266087896824104 1101 52-53 0.4350659651674249 1101 54-55 0.4271791279748882 1101 56-57 0.5126537881883735 1101 58-59 0.4984956995866554 1101 60-61 0.5084470900457418 1101 62-63 0.5030104631322629 1101 64-65 0.5330201845259097 1101 66-67 0.6061795664204794 1101 68-69 0.6337825463614024 1101 70-71 0.5063699794665837 1101 72-73 0.617211426098109 1101 74-75 0.5715430163766229 1101 76-77 0.5886173264439236 1101 78-79 0.5331624980961926 1101 80-81 0.5270614986908093 1101 82-83 0.4844120197375794 1101 84-85 0.4154160044894688 1101 86-87 0.5138710864675282 1101 88-89 0.47663709761512507 1101 90-91 0.4832412573676663 1101 92-93 0.4944837558652537 1101 94-95 0.42522780138030924 1101 96-97 0.49663819446534063 1101 98-99 0.531457449095651 1101 100 0.6093218230004993 1102 1 1.2488067908310327 1102 2 1.1593517295637135 1102 3 1.1116113359692932 1102 4 0.5557047219709261 1102 5 0.8668514559996936 1102 6 0.5496370038439551 1102 7 0.34647536463361917 1102 8 0.26558884626126655 1102 9 0.2931030442900422 1102 10-11 0.2771284544897483 1102 12-13 0.23896895175347765 1102 14-15 0.3708793226558029 1102 16-17 0.33634587349641265 1102 18-19 0.3296774770017663 1102 20-21 0.384521577170581 1102 22-23 0.42355592114678586 1102 24-25 0.39152116792576663 1102 26-27 0.34643606906156776 1102 28-29 0.45509870435034827 1102 30-31 0.4672842087249265 1102 32-33 0.4002975836858056 1102 34-35 0.3877667253216899 1102 36-37 0.5012126043471525 1102 38-39 0.46881568512324634 1102 40-41 0.39710338185656724 1102 42-43 0.44145899403130784 1102 44-45 0.4633476505352121 1102 46-47 0.4291294277388644 1102 48-49 0.46550760096067734 1102 50-51 0.5146274904021766 1102 52-53 0.4523319275537574 1102 54-55 0.4132002224525593 1102 56-57 0.42697273224769816 1102 58-59 0.47861880748109087 1102 60-61 0.5678921660812151 1102 62-63 0.5282525321582128 1102 64-65 0.46056857480382973 1102 66-67 0.5289247499921146 1102 68-69 0.4748771592279084 1102 70-71 0.5351911416497472 1102 72-73 0.5079674518277955 1102 74-75 0.5313389667448405 1102 76-77 0.5302829691775912 1102 78-79 0.5554700215581754 1102 80-81 0.5363559604791561 1102 82-83 0.5458804072479033 1102 84-85 0.5602514919259747 1102 86-87 0.4017148346905586 1102 88-89 0.37530172993569977 1102 90-91 0.418949830264868 1102 92-93 0.44065284809331473 1102 94-95 0.4183529694676906 1102 96-97 0.46070162582186214 1102 98-99 0.5215575744992798 1102 100 0.49337324153539797 1103 1 1.7779150622753548 1103 2 1.3003154152031051 1103 3 1.1796569356925417 1103 4 0.5886210587229144 1103 5 0.8849716547192727 1103 6 0.48831439698728474 1103 7 0.3266715305384693 1103 8 0.2121473398416498 1103 9 0.22593708728054906 1103 10-11 0.1412009472225435 1103 12-13 0.1457127451403437 1103 14-15 0.23987990372086898 1103 16-17 0.2998684046251867 1103 18-19 0.302560696418837 1103 20-21 0.3701983492827807 1103 22-23 0.3851867468171406 1103 24-25 0.34900713633336977 1103 26-27 0.3413995662895104 1103 28-29 0.42872771909082275 1103 30-31 0.39203771023235134 1103 32-33 0.3559372626181627 1103 34-35 0.3525639533377003 1103 36-37 0.4056961740122844 1103 38-39 0.42163204702271173 1103 40-41 0.3473526377481235 1103 42-43 0.3908183561729075 1103 44-45 0.45041901773018367 1103 46-47 0.3243756459499494 1103 48-49 0.33988614811115525 1103 50-51 0.4321299409188626 1103 52-53 0.40800936663211473 1103 54-55 0.1963128211317553 1103 56-57 0.29494950729247904 1103 58-59 0.38198060236226183 1103 60-61 0.41077959491112637 1103 62-63 0.44248068492927217 1103 64-65 0.44888336673764684 1103 66-67 0.44979596349939754 1103 68-69 0.4715646471764501 1103 70-71 0.41099970834986976 1103 72-73 0.3735282263286095 1103 74-75 0.3028071019827365 1103 76-77 0.38230076238123445 1103 78-79 0.34604368771021754 1103 80-81 0.4201044737545061 1103 82-83 0.4067763114788292 1103 84-85 0.40190438457375066 1103 86-87 0.3547795571379133 1103 88-89 0.4581330868952058 1103 90-91 0.46992985527739606 1103 92-93 0.4161390521449917 1103 94-95 0.45610507034619374 1103 96-97 0.5011551991384451 1103 98-99 0.4962406293288275 1103 100 0.534495986923794 1104 1 1.8300992637730573 1104 2 1.3287706825316086 1104 3 1.2042949957785964 1104 4 0.6228607721095969 1104 5 0.956007289339631 1104 6 0.5945495925353441 1104 7 0.34033758110979306 1104 8 0.27346498544604714 1104 9 0.23567782002233173 1104 10-11 0.18924389011859688 1104 12-13 0.2110999598763783 1104 14-15 0.3113101264062479 1104 16-17 0.30739729445345176 1104 18-19 0.29355162622098874 1104 20-21 0.29307129061731274 1104 22-23 0.31907678009277163 1104 24-25 0.28550064078758197 1104 26-27 0.3108956189133849 1104 28-29 0.3544677407328791 1104 30-31 0.3959505969021677 1104 32-33 0.39331529860957914 1104 34-35 0.4356580932952383 1104 36-37 0.43307170632451886 1104 38-39 0.41918728343222256 1104 40-41 0.3840412584452082 1104 42-43 0.4252043771437428 1104 44-45 0.39592302554854086 1104 46-47 0.4704516179700633 1104 48-49 0.3931719559888691 1104 50-51 0.41398396832175166 1104 52-53 0.5982079486449265 1104 54-55 0.5380903909827168 1104 56-57 0.5244576907342733 1104 58-59 0.4087095897455413 1104 60-61 0.4279452817697802 1104 62-63 0.4582170688214262 1104 64-65 0.46203659302550193 1104 66-67 0.5596108034003962 1104 68-69 0.5199444153051047 1104 70-71 0.574301279810463 1104 72-73 0.6726278489572337 1104 74-75 0.5687979975135136 1104 76-77 0.5883387604797363 1104 78-79 0.6136467706876765 1104 80-81 0.5492969057392365 1104 82-83 0.6567319767913844 1104 84-85 0.634121252292875 1104 86-87 0.6146865371686729 1104 88-89 0.528561245945756 1104 90-91 0.5746322483286121 1104 92-93 0.5901902119441544 1104 94-95 0.5306478809547635 1104 96-97 0.5344746639916593 1104 98-99 0.5328557846759523 1104 100 0.5631269105894923 1105 1 1.5592606574305137 1105 2 1.2389199298844709 1105 3 1.1800013031352705 1105 4 0.5656002409634553 1105 5 0.9569794391232946 1105 6 0.6323833796609648 1105 7 0.3511808643168095 1105 8 0.2739085953714735 1105 9 0.22657551015086597 1105 10-11 0.20932723100091977 1105 12-13 0.18711748638087045 1105 14-15 0.26547330323115403 1105 16-17 0.2548876329964429 1105 18-19 0.29617683993774335 1105 20-21 0.3127377164184324 1105 22-23 0.3162673508839191 1105 24-25 0.3480015474191376 1105 26-27 0.283795596913464 1105 28-29 0.34426808316121793 1105 30-31 0.333323619580149 1105 32-33 0.2854279201546319 1105 34-35 0.24217055184222858 1105 36-37 0.24709080322018195 1105 38-39 0.2651442785497977 1105 40-41 0.19880079869197687 1105 42-43 0.2504112320908263 1105 44-45 0.2596944502677303 1105 46-47 0.2748812165632657 1105 48-49 0.21213426389207513 1105 50-51 0.16408083118097494 1105 52-53 0.1475214517518566 1105 54-55 0.07377583001359511 1105 56-57 0.09988281440482893 1105 58-59 0.062334535911944045 1105 60-61 0.06078108379936964 1105 62-63 0.13460766104068256 1105 64-65 0.08170590800931876 1105 66-67 0.17167657203283682 1105 68-69 0.13292036677164987 1105 70-71 0.14019339471928305 1105 72-73 0.12414862744751076 1105 74-75 0.19454994492798505 1105 76-77 0.1410946946653553 1105 78-79 0.10896488023525208 1105 80-81 0.19251950011092944 1105 82-83 0.14232500711967333 1105 84-85 0.13331179960941242 1105 86-87 0.05687712150286117 1105 88-89 -0.015165811761754355 1105 90-91 0.025561978289346143 1105 92-93 0.08235769786465852 1105 94-95 0.01530273949164851 1105 96-97 0.11292154170308777 1105 98-99 -0.03673018039684095 1105 100 0.008332808494806443 1106 1 1.6252873123023335 1106 2 1.2826946662795642 1106 3 1.1478080667839947 1106 4 0.6242973060337391 1106 5 0.9705070894796961 1106 6 0.6278047975486345 1106 7 0.4267411148083795 1106 8 0.29239490591443484 1106 9 0.2285581272548498 1106 10-11 0.14309009804358652 1106 12-13 0.1705812669166633 1106 14-15 0.21645555390269777 1106 16-17 0.21904251216490422 1106 18-19 0.24471399224600532 1106 20-21 0.2704599418102589 1106 22-23 0.27129967987083603 1106 24-25 0.25219719881307157 1106 26-27 0.22029626713779749 1106 28-29 0.3381396918231374 1106 30-31 0.36485624824175034 1106 32-33 0.34943422655685197 1106 34-35 0.20075930466031622 1106 36-37 0.270335588910136 1106 38-39 0.28883142994639144 1106 40-41 0.2827052098209393 1106 42-43 0.34085857963169985 1106 44-45 0.3179836754484029 1106 46-47 0.3218987917922007 1106 48-49 0.3197136512146912 1106 50-51 0.3457120378141667 1106 52-53 0.3584973089714225 1106 54-55 0.3982888366005568 1106 56-57 0.4340444928653753 1106 58-59 0.401554922965758 1106 60-61 0.43199987454880073 1106 62-63 0.4142648967284899 1106 64-65 0.33524351027072896 1106 66-67 0.3884514137110884 1106 68-69 0.35672350609184633 1106 70-71 0.39893930764481667 1106 72-73 0.40912507401748854 1106 74-75 0.44512197360499783 1106 76-77 0.4082738765975904 1106 78-79 0.467450702593748 1106 80-81 0.4847794236365388 1106 82-83 0.5516011001382282 1106 84-85 0.5515871027102044 1106 86-87 0.5435883874475245 1106 88-89 0.5196929411798514 1106 90-91 0.5722102684379564 1106 92-93 0.6186006596268712 1106 94-95 0.6282296288684641 1106 96-97 0.6556873831419523 1106 98-99 0.6366310343472712 1106 100 0.6412180904389793 1107 1 1.6179043968553373 1107 2 1.2775282402532149 1107 3 1.1768010580535702 1107 4 0.6097089902950827 1107 5 0.9566106819343005 1107 6 0.611140979959373 1107 7 0.3912338518882734 1107 8 0.2695691742351656 1107 9 0.23934245135253462 1107 10-11 0.17742505377338347 1107 12-13 0.05950778466288398 1107 14-15 0.16696393325280923 1107 16-17 0.1702595600789678 1107 18-19 0.17122062985393427 1107 20-21 0.25929586446309827 1107 22-23 0.26104695464013616 1107 24-25 0.24588970336370863 1107 26-27 0.21117255611331842 1107 28-29 0.29673542332979963 1107 30-31 0.2637999829534792 1107 32-33 0.19847114558866963 1107 34-35 0.24422821695680597 1107 36-37 0.18323865216021318 1107 38-39 0.2216092303064272 1107 40-41 0.24788308969529282 1107 42-43 0.20856424079491376 1107 44-45 0.2314780011441684 1107 46-47 0.20174475229229216 1107 48-49 0.22114073274413926 1107 50-51 0.1744336892619316 1107 52-53 0.13571951974783758 1107 54-55 0.16919898597944893 1107 56-57 0.21475876306815422 1107 58-59 0.21426334875491193 1107 60-61 0.12528394946821209 1107 62-63 0.11825030128542124 1107 64-65 0.17182917219122373 1107 66-67 0.1636868802145628 1107 68-69 0.13013724280257577 1107 70-71 0.11412339609503164 1107 72-73 0.11769892238234547 1107 74-75 0.07932693901793186 1107 76-77 0.035473842297299996 1107 78-79 0.04069544354022625 1107 80-81 0.050519332751896684 1107 82-83 0.009385345723874394 1107 84-85 0.06596056831497421 1107 86-87 0.06968122208280292 1107 88-89 -0.01981016672742797 1107 90-91 0.01862466275657937 1107 92-93 0.0626768430287612 1107 94-95 0.07504268057559926 1107 96-97 0.07007351646875293 1107 98-99 0.04252297283170137 1107 100 0.01855617607055393 1108 1 0.7673697322839423 1108 2 0.9174741124099164 1108 3 0.9982823514074326 1108 4 0.5468511142441415 1108 5 0.8541807491840459 1108 6 0.5411435725931213 1108 7 0.3335805558042111 1108 8 0.29894953476998865 1108 9 0.18989828028103517 1108 10-11 0.16839356121000293 1108 12-13 0.09398161228978097 1108 14-15 0.07712454426779658 1108 16-17 0.10028649172338078 1108 18-19 0.10512077112601759 1108 20-21 0.08711733781981224 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28-29 -0.6969903893516474 2214 30-31 -0.7089011471378441 2214 32-33 -0.7517351864114801 2214 34-35 -0.7346910319747764 2214 36-37 -0.7966983019508476 2214 38-39 -0.7551781704835037 2214 40-41 -0.7123129007855198 2214 42-43 -0.7504764898396559 2214 44-45 -0.6903953090141925 2214 46-47 -0.6989709306840695 2214 48-49 -0.6840110882354296 2214 50-51 -0.7387826478466764 2214 52-53 -0.6658145138184892 2214 54-55 -0.6512903049411989 2214 56-57 -0.6106667966821462 2214 58-59 -0.5663530307165558 2214 60-61 -0.5727683561922134 2214 62-63 -0.6015272937506566 2214 64-65 -0.5698922546892504 2214 66-67 -0.669871972998159 2214 68-69 -0.631041939594418 2214 70-71 -0.6103797748889122 2214 72-73 -0.6674191329136363 2214 74-75 -0.6550768038644819 2214 76-77 -0.659640225906891 2214 78-79 -0.646373296414172 2214 80-81 -0.7188155704988262 2214 82-83 -0.7992655220915736 2214 84-85 -0.7064970999839097 2214 86-87 -0.6370255646950049 2214 88-89 -0.6850515726756683 2214 90-91 -0.6548545732169515 2214 92-93 -0.6685739824238368 2214 94-95 -0.7075098057499254 2214 96-97 -0.7424780976804399 2214 98-99 -0.747787023554995 2214 100 -0.7540614742864165 2215 1 -3.5043826784707193 2215 2 -3.426952886583475 2215 3 -3.587311657083095 2215 4 -1.868105047221711 2215 5 -2.5599679371187847 2215 6 -1.3776336897232468 2215 7 -1.0195929966488464 2215 8 -0.7095707141103418 2215 9 -0.5886492856226511 2215 10-11 -0.5319404249600694 2215 12-13 -0.47443394564857044 2215 14-15 -0.6544897131030538 2215 16-17 -0.6598201686702154 2215 18-19 -0.6986077837341469 2215 20-21 -0.6383889753667589 2215 22-23 -0.7517588133732289 2215 24-25 -0.7472963553733649 2215 26-27 -0.7487376131084034 2215 28-29 -0.8553446784031493 2215 30-31 -0.8262900560663553 2215 32-33 -0.8162434570494241 2215 34-35 -0.8363645264266637 2215 36-37 -0.7596621619254265 2215 38-39 -0.7401633125357989 2215 40-41 -0.8194337225245008 2215 42-43 -0.8522431255880534 2215 44-45 -0.7581482376616933 2215 46-47 -0.8436091010093989 2215 48-49 -0.9703594255194332 2215 50-51 -0.8862957022284803 2215 52-53 -0.8171733719036212 2215 54-55 -0.813832173435479 2215 56-57 -0.7462198472850545 2215 58-59 -0.7008263894715796 2215 60-61 -0.7713987625957301 2215 62-63 -0.7897496218965827 2215 64-65 -0.7935150603507282 2215 66-67 -0.7796060758529464 2215 68-69 -0.7514229214213444 2215 70-71 -0.8161539770257136 2215 72-73 -0.8515268401519407 2215 74-75 -0.9121314089694295 2215 76-77 -0.798752785596335 2215 78-79 -0.8003643301150909 2215 80-81 -0.7729873902109432 2215 82-83 -0.8032562122561302 2215 84-85 -0.7578352170611034 2215 86-87 -0.7027114779864228 2215 88-89 -0.6956373021751361 2215 90-91 -0.7217588811629483 2215 92-93 -0.7522736972886577 2215 94-95 -0.8086203299864145 2215 96-97 -0.8403464974880563 2215 98-99 -0.83011242119834 2215 100 -0.8431586607235992 2216 1 -6.114828863776847 2216 2 -6.186747928072602 2216 3 -5.520977513650841 2216 4 -2.929551121154212 2216 5 -3.9964189918368795 2216 6 -4.315852270111165 2216 7 -2.0718375591007145 2216 8 -1.3363942940312157 2216 9 -0.991686944281085 2216 10-11 -0.9161954798858858 2216 12-13 -0.7610139578049484 2216 14-15 -0.9978584176350154 2216 16-17 -0.9296324069340187 2216 18-19 -0.9133979367046976 2216 20-21 -0.9591155388925117 2216 22-23 -1.0489762321766207 2216 24-25 -1.528198537316726 2216 26-27 -1.3543711755687582 2216 28-29 -2.191926490991044 2216 30-31 -1.720861583576955 2216 32-33 -1.464339706893469 2216 34-35 -1.3885519527355328 2216 36-37 -1.4850336468788825 2216 38-39 -1.5074230002454385 2216 40-41 -1.573463423030681 2216 42-43 -1.549292067277264 2216 44-45 -1.6669229448078298 2216 46-47 -1.6835931575963485 2216 48-49 -1.7293105300453817 2216 50-51 -1.6572688513848846 2216 52-53 -1.6808508214539728 2216 54-55 -1.5612688039617808 2216 56-57 -1.5460347866043875 2216 58-59 -1.6016528242807695 2216 60-61 -1.6689035441498987 2216 62-63 -1.790030403478191 2216 64-65 -1.836383395977947 2216 66-67 -1.7246203257988952 2216 68-69 -1.8196183681014624 2216 70-71 -1.8150363418112576 2216 72-73 -1.789695224835814 2216 74-75 -1.8191130741260757 2216 76-77 -1.787424152056083 2216 78-79 -1.716817693958049 2216 80-81 -1.805163877571509 2216 82-83 -1.7373755244439302 2216 84-85 -1.7508562586756469 2216 86-87 -1.7721555708726875 2216 88-89 -1.7634493100163624 2216 90-91 -1.6910881424454907 2216 92-93 -1.6982142917037741 2216 94-95 -1.7945112545377988 2216 96-97 -1.947393072079322 2216 98-99 -1.9237100377372904 2216 100 -1.8769687232822676 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2934.0 3 1953.0 4 2415.0 5 3245.0 6 2560.0 7 2047.0 8 2063.0 9 2272.0 10 2674.0 11 6116.0 12 4653.0 13 6533.0 14 5980.0 15 6267.0 16 7393.0 17 9544.0 18 11998.0 19 18704.0 20 39770.0 21 59973.0 22 27287.0 23 15903.0 24 16055.0 25 18112.0 26 18559.0 27 19444.0 28 22057.0 29 25599.0 30 30111.0 31 36788.0 32 48403.0 33 65963.0 34 97784.0 35 195888.0 36 322492.0 37 625344.0 38 774768.0 39 274439.0 40 2234.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 14.45299767602325 42.587614770163356 24.318222545819875 18.64116500799352 2 18.061088930601723 39.82559820387234 24.39481526087993 17.718497604646014 3 18.773384140880943 23.14710872241676 19.493435869808952 38.58607126689335 4 35.47321310208468 24.074643410174904 21.768937972973355 18.683205514767064 5 17.926210831635537 39.16363954350207 22.819642697210814 20.09050692765158 6 38.41995928489783 21.07778671745595 22.902809088197486 17.59944490944874 7 19.31270898529223 21.75844508596338 40.282034069450454 18.646811859293933 8 37.089979847154275 22.10946281172391 22.389155822818548 18.41140151830327 9 36.945849627898646 23.2183276663738 22.814953439733966 17.02086926599359 10-11 17.124683216727004 42.023689818229506 22.629766556994195 18.22186040804929 12-13 17.083608924791385 22.669924169453136 31.986120062447025 28.260346843308454 14-15 17.629063272787786 32.13505025169921 22.124956193267195 28.11093028224581 16-17 27.531551123035058 22.1889219071473 31.927457141048958 18.352069828768684 18-19 17.952603440227563 22.76959543409004 31.831747712884706 27.446053412797696 20-21 27.796965367849367 32.22963949111596 22.507513246018437 17.465881895016224 22-23 27.06103517458428 22.689143181304953 32.109419112453146 18.14040253165762 24-25 17.538151211438908 32.13449337743908 22.492555216214292 27.834800194907714 26-27 17.994725187932808 27.892402296094666 36.11943429756146 17.993438218411068 28-29 29.289859413201857 22.247844499708332 30.644145936391215 17.818150150698596 30-31 25.11530559464402 25.176902987455925 31.091998015626064 18.61579340227399 32-33 19.902871629810736 26.288846896770078 32.00365342011 21.804628053309187 34-35 21.273357255583083 26.137167946536415 32.267617678954004 20.321857118926495 36-37 21.62100589472719 24.593549479207994 33.98536809864631 19.800076527418504 38-39 23.61699182641063 24.755493214536664 32.20655174329058 19.42096321576213 40-41 19.42075520526665 28.64661304209555 32.1469067385171 19.785725014120697 42-43 19.45176803723776 24.752644735202097 34.044472557638365 21.75111466992178 44-45 19.49942042143956 26.76138552331757 31.7453732882599 21.993820766982974 46-47 21.66624122797121 24.60654559762619 33.72827442065122 19.998938753751382 48-49 19.797868212373423 24.85749208279554 33.72178159887069 21.62285810596034 50-51 21.82819960318006 26.797863364041568 31.658854736892582 19.71508229588579 52-53 21.64962811106937 25.114168694128736 33.098352815244425 20.13785037955747 54-55 20.186716812305757 27.33199436108386 30.15169925592541 22.32958957068497 56-57 20.5376096322857 27.987360234206594 30.685563083619094 20.789467049888607 58-59 24.66990495470058 26.46451549887719 28.366214868421263 20.49936467800097 60-61 22.52741489135327 28.50651538356166 28.131757199290362 20.834312525794708 62-63 20.44682971752213 28.42345625081753 27.935389608901477 23.19432442275886 64-65 22.099533330077513 28.351436355683262 28.371729418541157 21.177300895698064 66-67 22.403334918216338 26.413441500275987 30.490627946864286 20.69259563464339 68-69 23.970050064054277 26.43274177514701 29.144898760395048 20.452309400403664 70-71 19.860825775527573 30.103817391965087 29.243987884004202 20.791368948503138 72-73 19.92105550795615 26.400039489875432 30.95621912649806 22.722685875670358 74-75 20.007873547928945 28.334353227528787 28.635332202546582 23.02244102199569 76-77 22.231097836279652 26.40112224118207 30.20197908367816 21.16580083886012 78-79 20.735357615813474 26.8184487702342 29.74938515677026 22.69680845718207 80-81 22.763636966261153 28.644786920528986 27.874039430509317 20.717536682700548 82-83 22.32671852518287 27.193175000365834 29.491141615498385 20.98896485895291 84-85 20.823075811052387 29.092248944865013 27.373974394685717 22.71070084939689 86-87 20.918100546800318 29.13653717110262 28.904833754297055 21.040528527800003 88-89 24.5619664976574 27.256710941968628 27.344271799056475 20.837050761317496 90-91 22.430435659597038 29.289701078217302 27.31028933010602 20.969573932079644 92-93 20.675438698568428 29.188128629667986 27.01640189418904 23.120030777574545 94-95 22.25984344002492 29.558377323185127 26.775764672407163 21.40601456438279 96-97 22.640805886534427 27.655823347550378 28.537036958680297 21.166333807234896 98-99 24.36850331782049 27.791531381107244 26.83098113510278 21.008984165969487 100 20.59594842241376 31.231224983541285 26.936583773025895 21.23624282101906 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 39305.0 1 21322.0 2 2651.5 3 1689.5 4 1383.0 5 1336.5 6 1476.5 7 1815.0 8 1734.0 9 1625.0 10 1726.0 11 2348.0 12 21072.5 13 24357.0 14 8902.0 15 8427.5 16 9027.5 17 9924.0 18 10902.5 19 11707.5 20 12547.0 21 13677.5 22 15159.0 23 17106.5 24 18922.0 25 21140.5 26 24268.0 27 27353.5 28 29943.5 29 32363.0 30 35073.5 31 37867.0 32 41321.5 33 45021.0 34 49152.5 35 52580.5 36 55654.0 37 58951.5 38 61112.5 39 62661.5 40 64095.5 41 65524.5 42 66165.0 43 67482.5 44 70211.0 45 72480.5 46 75514.5 47 82990.5 48 91094.5 49 117962.0 50 215997.0 51 218743.5 52 115609.0 53 79656.0 54 73344.0 55 70846.0 56 66602.0 57 59438.5 58 54109.5 59 47776.0 60 39405.0 61 32203.0 62 26303.5 63 20858.5 64 15994.0 65 12020.5 66 9110.5 67 6994.0 68 5357.0 69 4126.0 70 3107.5 71 2464.0 72 2073.5 73 1643.0 74 1232.0 75 905.5 76 659.5 77 481.5 78 341.5 79 199.0 80 103.5 81 63.5 82 28.5 83 11.0 84 7.5 85 7.0 86 7.5 87 5.5 88 4.5 89 4.5 90 2.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05387254770611538 2 0.0 3 0.0 4 4.230828353883407E-4 5 0.010506557078810461 6 0.0010577070884708517 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.051380589805678E-5 16-17 5.112250927609116E-4 18-19 0.0 20-21 0.0 22-23 1.7628451474514195E-5 24-25 0.003878259324393123 26-27 0.007192408201601792 28-29 0.001181106248792451 30-31 0.005817388986589685 32-33 0.0027324099785497004 34-35 0.026407420308822267 36-37 0.026054851279331978 38-39 0.02349872581552742 40-41 0.00218592798283976 42-43 0.006011301952809341 44-45 0.007456834973719505 46-47 0.001269248506165022 48-49 0.008937624897578697 50-51 0.009766162116880864 52-53 0.0021330426284162174 54-55 0.01121169513779103 56-57 0.006892724526535051 58-59 0.012710113513124736 60-61 0.008056202323852986 62-63 0.00109296399141988 64-65 0.013379994669156275 66-67 0.00537667769972683 68-69 0.0020272719195691324 70-71 0.00840877135334327 72-73 0.005429563054150373 74-75 0.00865556967398647 76-77 0.007527348779617561 78-79 0.01546015194314895 80-81 0.008673198125460984 82-83 0.01193446164824611 84-85 0.006839839172111508 86-87 0.013080310994089534 88-89 0.020519517516334525 90-91 0.018756672368883103 92-93 0.017381653153870996 94-95 0.005588219117421 96-97 0.01029501566111629 98-99 0.017681336828937737 100 0.01554829420052152 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2836324.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.852333291804634 #Duplication Level Percentage of deduplicated Percentage of total 1 84.3162944461041 46.24945484887637 2 9.843265402576302 10.798521490836096 3 2.4323585103040655 4.00261619077068 4 1.0254421318524491 2.249915743513168 5 0.5824355654173966 1.5973974877637858 6 0.3645827487461453 1.1998928667999498 7 0.2523647215999495 0.9689955674205744 8 0.17280842422252063 0.7583156224868213 9 0.13567899013069706 0.6698078268610019 >10 0.8249243052189076 8.552385077493609 >50 0.027389654003363077 0.9987980843712271 >100 0.016136977470851868 1.758957390355261 >500 0.0014828246339310873 0.566065917946146 >1k 0.0036748262666976557 5.536143773882017 >5k 9.670595438678041E-4 3.1411406944234477 >10k+ 1.9341190877356085E-4 10.951591416199737 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 184582 6.507789660137559 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG 86903 3.063930636979414 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 38200 1.3468136926528844 No Hit GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT 9074 0.3199211373594836 No Hit TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG 6744 0.23777255348824744 No Hit AGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTC 6543 0.23068591599549274 No Hit CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA 6342 0.22359927850273803 No Hit CCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATT 6128 0.216054301271646 No Hit CTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCG 6105 0.21524339250381833 No Hit CCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGAT 5648 0.19913098785611233 No Hit TCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGA 5500 0.19391296621965615 No Hit CCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTC 5467 0.19274948842233822 No Hit TACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACT 5384 0.18982316547756886 No Hit CGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAG 5311 0.1872494115622898 No Hit TCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCA 5184 0.1827717848877632 No Hit TCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGT 5152 0.18164356399339426 No Hit ATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTA 5126 0.18072688451671953 No Hit ACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTC 5116 0.18037431548722926 No Hit TCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACT 4982 0.17564989049205945 No Hit TGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCA 4824 0.17007929982611297 No Hit ACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAG 4719 0.16637732501646496 No Hit CTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGC 4717 0.16630681121056692 No Hit CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 4518 0.15929068752371026 No Hit ACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGA 4478 0.15788041140574913 No Hit GTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCAC 4419 0.15580025413175644 No Hit CAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTT 4351 0.1534027847312225 No Hit AACGAGTGGAATCACTGCTGAGTACATGGTAACGAGTGGAATCACTGCTG 4221 0.14881938734784883 No Hit GATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGT 4183 0.14747962503578574 No Hit TGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATG 4171 0.1470565422003974 No Hit ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 3943 0.13901796832801894 No Hit GTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCAT 3853 0.13584484706260638 No Hit GAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGT 3844 0.13552753493606512 No Hit ATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCC 3844 0.13552753493606512 No Hit TTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTAC 3709 0.1307678530379463 No Hit CGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAG 3619 0.12759473177253375 No Hit TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 3520 0.12410429838057993 No Hit CTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACG 3431 0.12096643401811641 No Hit CTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGT 3303 0.11645355044064076 No Hit TGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGA 3205 0.11299837395163598 No Hit ATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGG 3184 0.11225797898970638 No Hit TGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTG 3170 0.11176438234842001 No Hit CAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCC 3040 0.1071809849650463 No Hit ATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCAC 2987 0.1053123691087478 No Hit GCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCC 2961 0.10439568963207307 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0022211848857887884 0.0 0.0 7.051380589805678E-5 0.0 2 0.0022211848857887884 0.0 0.0 7.051380589805678E-5 0.0 3 0.0022211848857887884 0.0 0.0 7.051380589805678E-5 0.0 4 0.0022211848857887884 0.0 0.0 7.051380589805678E-5 0.0 5 0.0022211848857887884 0.0 0.0 7.051380589805678E-5 0.0 6 0.0022211848857887884 0.0 0.0 7.051380589805678E-5 0.0 7 0.0022211848857887884 0.0 0.0 7.051380589805678E-5 0.0 8 0.0022211848857887884 0.0 0.0 7.051380589805678E-5 0.0 9 0.0022211848857887884 0.0 0.0 7.051380589805678E-5 0.0 10-11 0.0022211848857887884 0.0 0.0 8.814225737257097E-5 0.0 12-13 0.0022740702402123314 0.0 0.0 1.0577070884708517E-4 0.0 14-15 0.0024679832064319874 0.0 0.0 1.0577070884708517E-4 0.0 16-17 0.0025737539152790724 0.0 0.0 1.7628451474514193E-4 0.0 18-19 0.002714781527075186 0.0 0.0 2.644267721177129E-4 0.0 20-21 0.00306735055656547 0.0 0.0 2.820552235922271E-4 0.0 22-23 0.0034728049404792963 0.0 0.0 3.3494057801576973E-4 0.0 24-25 0.00403691538766375 0.0 0.0 4.2308283538834067E-4 0.0 26-27 0.004530512028950148 0.0 0.0 4.2308283538834067E-4 0.0 28-29 0.004883081058440432 0.0 0.0 4.2308283538834067E-4 0.0 30-31 0.005517705311522943 0.0 0.0 4.4071128686285486E-4 0.0 32-33 0.006363870982299624 0.0 0.0 4.583397383373691E-4 0.0 34-35 0.007262922007499848 0.0 0.0 4.583397383373691E-4 0.0 36-37 0.008161973032700073 0.0 0.0 4.583397383373691E-4 0.0 38-39 0.009096280960849325 0.0 0.0 4.583397383373691E-4 0.0 40-41 0.010700470045030117 0.0 0.0 4.583397383373691E-4 0.0 42-43 0.012198888420363823 0.0 0.0 4.583397383373691E-4 0.0 44-45 0.0140322473737133 0.0 0.0 4.583397383373691E-4 0.0 46-47 0.015618808006419578 0.0 0.0 4.583397383373691E-4 0.0 48-49 0.016958570318482656 0.0 0.0 5.4648199570994E-4 0.0 50-51 0.0192502690101695 0.0 0.0 5.993673501334826E-4 0.0 52-53 0.02164773841070343 0.0 0.0 6.34624253082511E-4 0.0 54-55 0.024485919098100217 0.0 0.0 6.698811560315395E-4 0.0 56-57 0.02705967301337929 0.0 0.0 7.051380589805678E-4 0.0 58-59 0.029756826088979962 0.0 0.0 8.10908767827653E-4 0.0 60-61 0.033423543995678914 0.0 0.0 8.461656707766813E-4 0.0 62-63 0.038006941379052604 0.0 0.0 9.166794766747382E-4 0.0 64-65 0.04289002243749304 0.0 0.0 9.166794766747382E-4 0.0 66-67 0.049518320191910374 0.0 0.0 9.166794766747382E-4 0.0 68-69 0.05639341626697091 0.0 0.0 9.166794766747382E-4 0.0 70-71 0.0644496185908239 0.0 0.0 0.0010577070884708517 0.0 72-73 0.07358115645462224 0.0 0.0 0.0011282208943689085 0.0 74-75 0.08407008508195819 0.0 0.0 0.0011458493458434227 0.0 76-77 0.09632185885674556 0.0 0.0 0.0012339916032159937 0.0 78-79 0.11158809783367485 0.0 0.0 0.001269248506165022 0.0 80-81 0.13217812915590743 0.0 0.0 0.0013045054091140504 0.0 82-83 0.156470135287788 0.0 0.0 0.0013750192150121074 0.0 84-85 0.18557470867221093 0.0 0.0 0.0014102761179611357 0.0 86-87 0.2199149321445646 0.0 0.0 0.0014102761179611357 0.0 88 0.24993618500566225 0.0 0.0 0.0014102761179611357 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGAGT 1320 0.0 70.885155 1 CGTGTGG 1260 0.0 49.60436 3 TCGAGTG 800 0.0 47.58102 2 AACGTGT 990 0.0 47.019302 1 GTGGAAT 110245 0.0 46.717155 6 TGGAATC 111320 0.0 46.39523 7 AGTGGAA 110335 0.0 46.06723 5 GGAATCA 112400 0.0 46.00796 8 GAATCAC 112450 0.0 46.00085 9 ACGTGTG 1240 0.0 44.340736 2 AACGAGT 111900 0.0 43.99393 1 ATCGAGT 750 0.0 43.884678 1 ACGAGTG 114600 0.0 43.80739 2 CGAGTGG 117080 0.0 43.164433 3 GAGTGGA 119405 0.0 42.595516 4 GAGTACT 27040 0.0 39.502777 20-21 AGTACTT 27450 0.0 39.48626 20-21 GTACTTT 27245 0.0 38.43901 22-23 TACTTTT 28685 0.0 37.22201 22-23 ACTTTTT 29625 0.0 35.289017 24-25 >>END_MODULE