FastQCFastQC Report
Thu 26 May 2016
SRR2132714_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132714_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2892286
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG32152611.116673800585419No Hit
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1272734.400429279815343No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360801.2474561644318716No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT302751.046749871900635No Hit
TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG220060.760851451066734No Hit
AGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTC215340.7445321797360288No Hit
CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA209690.7249974587575364No Hit
CCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATT204840.7082287159706889No Hit
CCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGAT196400.6790476460488347No Hit
CTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCG192400.66521775509061No Hit
TCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGA187080.646824000116171No Hit
TACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACT185540.6414994920972545No Hit
TCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGT184210.6369010533536448No Hit
TCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCA178890.6185072983792059No Hit
CGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAG174890.6046774074209812No Hit
CCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTC174720.6040896370552566No Hit
ACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTC171190.5918847582846233No Hit
TGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCA170800.5905363439161964No Hit
ACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGA169160.5848660886233242No Hit
TCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACT166960.5772596485963006No Hit
ATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTA164930.5702409789350016No Hit
AACGAGTGGAATCACTGCTGAGTACATGGTAACGAGTGGAATCACTGCTG158570.5482514523114242No Hit
ACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAG154230.5332460206217504No Hit
CTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGC150810.5214214638524682No Hit
CAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTT146180.5054133650683231No Hit
GTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCAC142300.4919983708388451No Hit
TGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATG140720.48653556391034636No Hit
GATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGT138230.47792645678885143No Hit
GAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGT136390.47156470694806807No Hit
GTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCAT132870.4593944029048303No Hit
ATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCC130540.4513384914216644No Hit
CGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAG124190.42938353952548264No Hit
CTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACG121010.4183887762136939No Hit
TTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTAC117510.40628762162524734No Hit
TGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTG115190.39826628486947696No Hit
CTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGT114460.39574232976960094No Hit
ATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGG111230.38457469282083445No Hit
TGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGA110780.38301883008803417No Hit
ATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCAC99870.3452978024994762No Hit
CAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCC97570.337345615198497No Hit
ACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATC96800.3346833611890387No Hit
GCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCC96660.3341993150055009No Hit
TGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACA94670.32731894425378405No Hit
ACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAAC88350.30546771653978894No Hit
TACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAAT83720.2894596177556438No Hit
GAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATG83500.2886989737529414No Hit
AGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGA79990.27656324443709923No Hit
GGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACAT79300.27417758824680544No Hit
GGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGC77070.2664674240375952No Hit
TCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGA76750.2653610327609372No Hit
GGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTG74520.2576508685517269No Hit
TGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACT70510.24378640286610662No Hit
CACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAA67690.23403632974055819No Hit
AACGAGTGGAATCACTGCTGAGTACATGGAAACGAGTGGAATCACTGCTG66590.23023310972704636No Hit
GAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGG64670.2235947620670985No Hit
GAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCT60820.21028349201980717No Hit
AGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCA60120.20786326110211784No Hit
GTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTAC56060.19382592177951974No Hit
TACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG52430.18127529573493079No Hit
GCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAG46740.1616022758468561No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT45870.1585942745634422No Hit
GTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAA45060.1557937216444017No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGTAACGAGTGGAATCACTGCT43680.15102240926381416No Hit
CATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTC36150.12498763953495609No Hit
AACGTGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG30960.1070433560166595No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30420.10517632073729914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT17600.072.667461
AACGTGT14100.056.023971
CGTGTGG16850.055.7783853
TCGAGTG9250.052.8356972
ACGTGTG17000.052.521912
CGATTGG5800.046.1830643
GTGTGGA22400.046.1541254
ATCGAGT9650.044.3402141
ACGATTG6000.042.293962
TGTGGAA25100.040.627675
ATGAGTG15150.037.8427852
ATCTGCG4850.036.82585594
TGAGTGG16650.036.691463
AATGAGT14800.035.9004941
AGTACTT239100.034.12094520-21
GTACTTT227200.033.48859422-23
TACGTGT2950.033.4719471
GAGTACT238350.033.45935420-21
TACTTTT233800.033.4578222-23
CGTGCTA3200.033.0487386-87
Produced by FastQC (version 0.11.5)