FastQCFastQC Report
Thu 26 May 2016
SRR2132713_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132713_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3418918
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG40707411.906515453134588No Hit
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1407864.117852490173792No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450801.3185458089372135No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT373231.0916611629761228No Hit
TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG267090.7812120676775518No Hit
AGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTC266650.7799251108099112No Hit
CCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATT253340.7409946655637836No Hit
CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA252270.7378650204538395No Hit
CCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGAT244030.7137638282052977No Hit
CTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCG235550.6889606594834974No Hit
TCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGA227890.6665559103786637No Hit
TCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGT224050.6553242868065277No Hit
TCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCA221260.6471638103048977No Hit
TACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACT220900.6461108455950099No Hit
ACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTC215140.629263410236806No Hit
CCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTC214840.6283859396452328No Hit
ACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGA214740.6280934494480418No Hit
CGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAG209930.6140246709631526No Hit
TGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCA209730.6134396905687706No Hit
TCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACT203880.5963290140330947No Hit
ATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTA200450.586296600269442No Hit
ACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAG190940.558480782516574No Hit
CTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGC186640.5459037040373591No Hit
CAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTT180920.5291732647580316No Hit
GTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCAC176620.5165961862788169No Hit
AACGAGTGGAATCACTGCTGAGTACATGGTAACGAGTGGAATCACTGCTG176490.5162159490224685No Hit
GAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGT171570.5018254313206693No Hit
GATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGT171070.500362980334714No Hit
TGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATG170850.4997195019008938No Hit
ATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCC163490.4781922233876332No Hit
GTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCAT160650.46988550178740757No Hit
CGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAG152570.4462522938543715No Hit
CTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACG148110.43320723105965103No Hit
TTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTAC144380.4222973467044252No Hit
CTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGT141870.4149558427549301No Hit
TGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTG139430.40781908194346866No Hit
ATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGG138180.40416295447858064No Hit
TGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGA137320.40164753878273773No Hit
ATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCAC125260.36637322100149816No Hit
ACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATC120160.35145622094475504No Hit
CAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCC118860.3476538483812715No Hit
GCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCC116850.3417747954177316No Hit
TGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACA113780.33279534636396657No Hit
ACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAAC111060.3248396130003703No Hit
GGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACAT103570.3029320972307613No Hit
TACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAAT101680.2974040325038506No Hit
GAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATG101040.29553209524182794No Hit
AGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGA98830.28906806188390594No Hit
GGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGC95860.28038110302733205No Hit
TCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGA94890.27754394811457894No Hit
GGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTG89870.2628609402155887No Hit
TGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACT88900.2600237853028356No Hit
AACGAGTGGAATCACTGCTGAGTACATGGAAACGAGTGGAATCACTGCTG85880.25119058134766614No Hit
AGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCA81870.23946172444030536No Hit
CACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAA81540.23849650678957496No Hit
GAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGG80570.23565935187682185No Hit
GAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCT76030.22238029692434857No Hit
GTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTAC71130.20804827726198755No Hit
TACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG70760.2069660635323807No Hit
GCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAG58540.17122376143563547No Hit
GTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAA55890.1634727712100729No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGTAACGAGTGGAATCACTGCT52740.1542593299985551No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT49340.1443146632940597No Hit
CATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTC48190.14095102602636272No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT34710.10152334744501038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT22850.072.2180941
ACGTGTG14850.058.540232
AACGTGT13600.058.075841
CGTGTGG16700.057.4014473
TCGAGTG10400.052.8641972
ATCGAGT9250.052.858731
GTGTGGA23150.043.4382064
ATCTGCG6250.038.35201694
TGAGTGG20850.038.088143
CGATTGG7650.038.083723
ATGAGTG19700.037.2106672
TACGTGT2300.036.793391
ACGATTG7300.036.6911352
AATGAGT18200.036.4227641
TGTGGAA28000.035.914095
AGTACTT251400.034.75987220-21
GTACTTT238050.034.0747422-23
GAGTACT252650.033.8531720-21
TACTTTT245150.033.8355122-23
ATCGTGT1850.033.036711
Produced by FastQC (version 0.11.5)