##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2132713_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3418918 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.898340937103494 33.0 31.0 34.0 25.0 34.0 2 31.612308338486034 34.0 31.0 34.0 28.0 34.0 3 31.81603595055512 34.0 31.0 34.0 28.0 34.0 4 35.69536736476277 37.0 35.0 37.0 33.0 37.0 5 35.38835824667336 37.0 35.0 37.0 33.0 37.0 6 35.698671041540045 37.0 35.0 37.0 35.0 37.0 7 35.880292244505426 37.0 35.0 37.0 35.0 37.0 8 36.05686740658887 37.0 37.0 37.0 35.0 37.0 9 37.97357819052694 39.0 39.0 39.0 37.0 39.0 10-11 38.0660795315945 39.0 39.0 39.0 37.0 39.0 12-13 38.058518952487304 39.0 39.0 39.0 37.0 39.0 14-15 39.63486722992479 41.0 40.0 41.0 37.5 41.0 16-17 39.54808114731035 41.0 40.0 41.0 37.0 41.0 18-19 39.497731884765884 41.0 40.0 41.0 37.0 41.0 20-21 39.36231901437823 41.0 39.5 41.0 36.5 41.0 22-23 39.215211069701 40.5 39.0 41.0 36.0 41.0 24-25 38.83303372587468 40.0 38.5 41.0 35.0 41.0 26-27 38.88314109902606 40.0 39.0 41.0 35.5 41.0 28-29 38.76617426916937 41.0 39.0 41.0 35.0 41.0 30-31 38.5198260092813 41.0 39.0 41.0 35.0 41.0 32-33 38.40050785072938 41.0 38.5 41.0 35.0 41.0 34-35 38.197591021486915 41.0 38.0 41.0 34.0 41.0 36-37 37.73807970825858 40.0 37.5 41.0 33.5 41.0 38-39 37.52809075853823 40.0 37.0 41.0 33.0 41.0 40-41 37.560982597418246 40.0 37.0 41.0 33.0 41.0 42-43 37.51230228393895 40.0 37.0 41.0 33.0 41.0 44-45 37.3406013247466 40.0 37.0 41.0 33.0 41.0 46-47 37.23103201071216 40.0 37.0 41.0 33.0 41.0 48-49 37.05604609411516 40.0 36.5 41.0 33.0 41.0 50-51 36.7729386899598 40.0 36.0 41.0 31.5 41.0 52-53 36.22262350252331 39.5 35.5 41.0 29.5 41.0 54-55 35.399242684381434 39.0 35.0 41.0 28.0 41.0 56-57 35.32738281526495 39.0 35.0 41.0 26.0 41.0 58-59 35.38443419818785 39.5 35.0 41.0 26.0 41.0 60-61 35.08688187315402 39.0 35.0 41.0 25.0 41.0 62-63 34.95695962874804 39.0 35.0 41.0 24.5 41.0 64-65 34.39471961597207 38.5 35.0 40.0 22.0 41.0 66-67 33.54006735464261 37.5 33.0 40.0 20.0 41.0 68-69 33.41260451405971 37.0 34.0 39.0 20.5 41.0 70-71 33.27583931524535 37.0 34.0 39.0 20.5 41.0 72-73 32.92635842684732 36.0 34.0 39.0 19.5 40.5 74-75 32.5229188006264 36.0 33.0 38.5 18.0 40.0 76-77 32.09446614396718 35.0 33.0 37.5 14.0 40.0 78-79 31.68108565341433 35.0 32.0 37.0 11.0 39.0 80-81 31.234212110381122 35.0 32.0 36.0 7.5 39.0 82-83 30.502897407893375 35.0 30.5 36.0 6.5 38.0 84-85 30.090761316884464 34.0 30.5 35.5 2.0 37.0 86-87 30.17864555394426 34.5 31.0 35.0 2.0 37.0 88-89 30.276580339159935 35.0 32.0 35.0 2.0 36.5 90-91 29.992636559285714 35.0 31.0 35.0 2.0 36.0 92-93 29.89721470359921 35.0 31.5 35.0 2.0 36.0 94-95 29.426761770829252 34.0 31.0 35.0 2.0 35.5 96-97 28.779712177946358 34.0 30.0 35.0 2.0 35.0 98-99 28.715410694260584 34.0 30.0 35.0 2.0 35.0 100 28.87650157154983 34.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.9286450112199063 1101 2 1.473832168106103 1101 3 1.3440326492044576 1101 4 0.7321528004924005 1101 5 1.0475479898272155 1101 6 0.6991202794308862 1101 7 0.5135276924843595 1101 8 0.40379862544561007 1101 9 0.3876561223701458 1101 10-11 0.3109319672005171 1101 12-13 0.36464741241690035 1101 14-15 0.43061636488708643 1101 16-17 0.4785026957041367 1101 18-19 0.39822007453692265 1101 20-21 0.5150167936305579 1101 22-23 0.48869097067022693 1101 24-25 0.5209391511628425 1101 26-27 0.5829286950260624 1101 28-29 0.5598389998653346 1101 30-31 0.6147544627028338 1101 32-33 0.6005761417210707 1101 34-35 0.7540334028945708 1101 36-37 0.7388222383418537 1101 38-39 0.7049911684807029 1101 40-41 0.7260218715523621 1101 42-43 0.7030342771340159 1101 44-45 0.752473176987337 1101 46-47 0.6828444872240809 1101 48-49 0.6847633070895256 1101 50-51 0.6607393504198527 1101 52-53 0.6643132254101118 1101 54-55 0.5673126946761826 1101 56-57 0.5914022732263362 1101 58-59 0.5560218589596104 1101 60-61 0.4949419251387184 1101 62-63 0.5640041198237142 1101 64-65 0.6373952967910768 1101 66-67 0.7017644432882122 1101 68-69 0.7080015981328032 1101 70-71 0.637293999520665 1101 72-73 0.6605904472432513 1101 74-75 0.6391406395200789 1101 76-77 0.7197796262416745 1101 78-79 0.7039943050196591 1101 80-81 0.6728184252262857 1101 82-83 0.6667386661602244 1101 84-85 0.6145908252761849 1101 86-87 0.6614481113108326 1101 88-89 0.7191824150186186 1101 90-91 0.7147793225182006 1101 92-93 0.7836779547417088 1101 94-95 0.8686379424929527 1101 96-97 0.8258155799272018 1101 98-99 0.8326884499512772 1101 100 0.9019584669760512 1102 1 1.2850798004861659 1102 2 1.1879423027121128 1102 3 1.1453438591553748 1102 4 0.5772807257971024 1102 5 0.9092269458250328 1102 6 0.614077148127322 1102 7 0.3694444966556105 1102 8 0.29828779127459626 1102 9 0.30183358288581985 1102 10-11 0.23197277696611707 1102 12-13 0.24405060122700206 1102 14-15 0.3262688470883006 1102 16-17 0.3307881588698436 1102 18-19 0.31951344994598685 1102 20-21 0.39987083840611604 1102 22-23 0.4637921862291492 1102 24-25 0.37501105994586226 1102 26-27 0.3673703335491183 1102 28-29 0.4658501790325573 1102 30-31 0.5242413349450459 1102 32-33 0.5666897877070056 1102 34-35 0.5490076153510941 1102 36-37 0.6121360874833925 1102 38-39 0.6805410987943645 1102 40-41 0.6587701166803726 1102 42-43 0.6173080009869167 1102 44-45 0.6469344149969558 1102 46-47 0.6247429610236708 1102 48-49 0.7227348583871063 1102 50-51 0.7672659859874713 1102 52-53 0.7908796255184072 1102 54-55 0.6985243441712541 1102 56-57 0.7174314308980598 1102 58-59 0.7468774215128278 1102 60-61 0.7532908240441216 1102 62-63 0.7675993486397275 1102 64-65 0.7821554676354765 1102 66-67 0.8408380193269949 1102 68-69 0.8265310963135946 1102 70-71 0.7432395728514507 1102 72-73 0.8355542158638656 1102 74-75 0.8405725724364785 1102 76-77 0.8054753804372226 1102 78-79 0.824349020577035 1102 80-81 0.8759766713087522 1102 82-83 0.8241979319750108 1102 84-85 0.8493943756195037 1102 86-87 0.7948726724047006 1102 88-89 0.8057913037400724 1102 90-91 0.8183316570081267 1102 92-93 0.8546830449431901 1102 94-95 0.8096501111696881 1102 96-97 0.816784400888988 1102 98-99 0.8469418507650737 1102 100 0.873643210459381 1103 1 1.90951325816431 1103 2 1.3516115134818314 1103 3 1.2652277704380808 1103 4 0.68939393311976 1103 5 0.9623206236442883 1103 6 0.6213051650221075 1103 7 0.439995966116534 1103 8 0.3447524308050802 1103 9 0.34856274689236955 1103 10-11 0.28963213351262596 1103 12-13 0.26636726814538036 1103 14-15 0.3652647661804238 1103 16-17 0.3535623204119247 1103 18-19 0.3432849557172375 1103 20-21 0.3986475728834975 1103 22-23 0.4470504184093258 1103 24-25 0.3680697866678244 1103 26-27 0.43457959575008687 1103 28-29 0.5645302568155657 1103 30-31 0.593855733314328 1103 32-33 0.5492886766126688 1103 34-35 0.5787475575859062 1103 36-37 0.598164801661035 1103 38-39 0.5554387099735862 1103 40-41 0.5954257264070009 1103 42-43 0.6308557044064713 1103 44-45 0.6406394418315458 1103 46-47 0.6595436958342233 1103 48-49 0.640782292476807 1103 50-51 0.7430252696295625 1103 52-53 0.7252474875336503 1103 54-55 0.5288491692441468 1103 56-57 0.5640341906480586 1103 58-59 0.6079740859873297 1103 60-61 0.6896932133683222 1103 62-63 0.685980298667026 1103 64-65 0.697729941115071 1103 66-67 0.7320517793593595 1103 68-69 0.7800018816904313 1103 70-71 0.7192024442326073 1103 72-73 0.7748033095929401 1103 74-75 0.7660729607565813 1103 76-77 0.7291724028893114 1103 78-79 0.7399650948782117 1103 80-81 0.83486222236861 1103 82-83 0.8223704934643656 1103 84-85 0.7755460067241025 1103 86-87 0.7465904797073151 1103 88-89 0.8110069438183665 1103 90-91 0.7671190319596732 1103 92-93 0.7965335629855446 1103 94-95 0.8172735646969045 1103 96-97 0.8593094105798293 1103 98-99 0.8509081389969886 1103 100 0.8368112892199413 1104 1 1.9388770816545957 1104 2 1.4571299992425502 1104 3 1.336474111091107 1104 4 0.7265692214385311 1104 5 1.0259997790546436 1104 6 0.720599802704136 1104 7 0.4238792627811918 1104 8 0.4047287515961884 1104 9 0.4149462257304961 1104 10-11 0.36442387079240035 1104 12-13 0.367232274593313 1104 14-15 0.451040995650807 1104 16-17 0.3931370840710855 1104 18-19 0.4427911151862176 1104 20-21 0.47461397808944383 1104 22-23 0.4886452017632905 1104 24-25 0.486909866436406 1104 26-27 0.5160591385046942 1104 28-29 0.541988010954249 1104 30-31 0.5871917942358209 1104 32-33 0.6199217779004584 1104 34-35 0.6485698699255025 1104 36-37 0.6877647353432081 1104 38-39 0.6784334814277742 1104 40-41 0.6701244716496007 1104 42-43 0.6469131762932605 1104 44-45 0.6694829511411129 1104 46-47 0.6783855806243793 1104 48-49 0.6622726118787199 1104 50-51 0.7263334997925384 1104 52-53 0.8102230896587557 1104 54-55 0.7472480883704833 1104 56-57 0.8342381647707313 1104 58-59 0.820360010295424 1104 60-61 0.9082275759650642 1104 62-63 0.962169189494638 1104 64-65 0.9249487304536785 1104 66-67 0.8689568492638315 1104 68-69 0.8700659064740464 1104 70-71 0.9132119119050088 1104 72-73 0.8852932258733688 1104 74-75 0.8179381831385797 1104 76-77 0.8657093820988067 1104 78-79 0.8347741202534671 1104 80-81 0.8117549379032205 1104 82-83 0.8338234890313672 1104 84-85 0.7707920478772579 1104 86-87 0.8758284196562514 1104 88-89 0.8273792916901463 1104 90-91 0.8690570903046542 1104 92-93 0.9157480844509251 1104 94-95 0.9089923262916599 1104 96-97 1.031082550120697 1104 98-99 1.0531780539002504 1104 100 1.1175368009268425 1105 1 1.6160161318033985 1105 2 1.2996254900174442 1105 3 1.2242141635589476 1105 4 0.6558289043833696 1105 5 1.009363591809823 1105 6 0.7057421869990677 1105 7 0.43342092144970934 1105 8 0.31318486728137174 1105 9 0.30014126809040675 1105 10-11 0.3198264672068092 1105 12-13 0.2719455605084491 1105 14-15 0.3359088705995745 1105 16-17 0.3212436721224634 1105 18-19 0.35266927964193684 1105 20-21 0.3767770010601197 1105 22-23 0.4301090397666698 1105 24-25 0.43957664291545484 1105 26-27 0.40374320394941066 1105 28-29 0.4465740831122247 1105 30-31 0.437959162318279 1105 32-33 0.4655742450475273 1105 34-35 0.4979014843059062 1105 36-37 0.5303143541126474 1105 38-39 0.5610205228745997 1105 40-41 0.5539235348721405 1105 42-43 0.5418383525551107 1105 44-45 0.5778117017528643 1105 46-47 0.6753927175647476 1105 48-49 0.5536877044237514 1105 50-51 0.578570710972997 1105 52-53 0.5696007647144015 1105 54-55 0.4992437555369307 1105 56-57 0.598880695193948 1105 58-59 0.6018959872538474 1105 60-61 0.6115558639813301 1105 62-63 0.6612323471800394 1105 64-65 0.6414440206264231 1105 66-67 0.7447572907615054 1105 68-69 0.7361936277892269 1105 70-71 0.7643245188856156 1105 72-73 0.7369529060512718 1105 74-75 0.746177410716534 1105 76-77 0.7588789220454402 1105 78-79 0.7504503570262635 1105 80-81 0.7849364837157431 1105 82-83 0.8103179318329516 1105 84-85 0.7286875367498915 1105 86-87 0.8089480079376301 1105 88-89 0.8061473912787349 1105 90-91 0.8005507203216524 1105 92-93 0.8593257676344166 1105 94-95 0.8150942416963503 1105 96-97 0.8602651266132391 1105 98-99 0.8235123029132332 1105 100 0.8627118868312564 1106 1 1.6134128506588965 1106 2 1.3196280854957259 1106 3 1.1908777501485375 1106 4 0.6803783917764079 1106 5 1.0160456752889502 1106 6 0.6962636810089293 1106 7 0.4312329168304174 1106 8 0.3565805660153387 1106 9 0.3346463448787844 1106 10-11 0.28192369579453214 1106 12-13 0.2567539632872595 1106 14-15 0.3505953967818769 1106 16-17 0.38119324510057595 1106 18-19 0.37859796394800327 1106 20-21 0.4266089725887099 1106 22-23 0.40394286098421617 1106 24-25 0.42397258230611357 1106 26-27 0.4636121815773748 1106 28-29 0.5059101868722209 1106 30-31 0.5261976415979888 1106 32-33 0.5418790888999396 1106 34-35 0.4486985261177523 1106 36-37 0.5266665572301861 1106 38-39 0.5555714969181764 1106 40-41 0.5342929921707764 1106 42-43 0.5817510795978578 1106 44-45 0.5023712096633233 1106 46-47 0.5004662698053153 1106 48-49 0.47437580185603423 1106 50-51 0.48500899608464465 1106 52-53 0.5323223454592423 1106 54-55 0.5913444461312807 1106 56-57 0.5941910519736524 1106 58-59 0.6137322511778436 1106 60-61 0.6674318091543157 1106 62-63 0.6274684396053445 1106 64-65 0.596361302076474 1106 66-67 0.5323056685054439 1106 68-69 0.6074047633766853 1106 70-71 0.6376450965930829 1106 72-73 0.6885630471434467 1106 74-75 0.6768174630532044 1106 76-77 0.7108939084684636 1106 78-79 0.6347910398341803 1106 80-81 0.7461683543820747 1106 82-83 0.831600360937788 1106 84-85 0.7347237691339039 1106 86-87 0.7216476174659903 1106 88-89 0.6526833107269212 1106 90-91 0.6696529066014278 1106 92-93 0.748810405031314 1106 94-95 0.7041341980988847 1106 96-97 0.7545817889267283 1106 98-99 0.7075155119428693 1106 100 0.771684849504009 1107 1 1.6248789866101774 1107 2 1.2866572572978647 1107 3 1.2213029644503237 1107 4 0.6920171914512068 1107 5 0.9825239271979171 1107 6 0.6470392507500549 1107 7 0.4267263859349626 1107 8 0.38409552130418945 1107 9 0.37282365139795814 1107 10-11 0.27767810767461754 1107 12-13 0.2021336952728987 1107 14-15 0.30922882394664697 1107 16-17 0.3043008219649508 1107 18-19 0.2703838671001577 1107 20-21 0.3532177710739859 1107 22-23 0.3271055568286627 1107 24-25 0.33229984753906905 1107 26-27 0.3239282124864218 1107 28-29 0.2895509931169542 1107 30-31 0.34194680570391256 1107 32-33 0.3868794850856858 1107 34-35 0.3798403064396041 1107 36-37 0.3414910180271704 1107 38-39 0.3924631566009822 1107 40-41 0.41954294874457787 1107 42-43 0.3719664677605934 1107 44-45 0.38097901103294873 1107 46-47 0.32785201648819395 1107 48-49 0.39826931098401985 1107 50-51 0.3546455520564322 1107 52-53 0.3167196254850566 1107 54-55 0.3144828117368803 1107 56-57 0.2783988954488592 1107 58-59 0.25104940085373784 1107 60-61 0.31640983308779624 1107 62-63 0.298273232623842 1107 64-65 0.3751539708472649 1107 66-67 0.33571417687864624 1107 68-69 0.42486666447831567 1107 70-71 0.41969381452252463 1107 72-73 0.4347783743841944 1107 74-75 0.32968986753050444 1107 76-77 0.3926676291499973 1107 78-79 0.4143045545878117 1107 80-81 0.38192779039865243 1107 82-83 0.40647187047873246 1107 84-85 0.3776086876825495 1107 86-87 0.34123953265486406 1107 88-89 0.32956032985237727 1107 90-91 0.3174181201821753 1107 92-93 0.313170325994669 1107 94-95 0.3422816991115205 1107 96-97 0.38300292029825656 1107 98-99 0.3800708675740374 1107 100 0.35947103104653166 1108 1 0.7302143582849183 1108 2 0.9150830669918584 1108 3 0.9973854111008009 1108 4 0.5173962557079506 1108 5 0.8636924355310072 1108 6 0.6178823130531512 1108 7 0.37508535283340194 1108 8 0.294492571309803 1108 9 0.2515099755042982 1108 10-11 0.1699901057925075 1108 12-13 0.15419990026405372 1108 14-15 0.10760199428717243 1108 16-17 0.13719020071476962 1108 18-19 0.11125857932910321 1108 20-21 0.10807317817072004 1108 22-23 0.11310461390637983 1108 24-25 0.11308705166274535 1108 26-27 0.16937354581279607 1108 28-29 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-6.271236297266633 2216 3 -5.568088914594572 2216 4 -3.228736066325297 2216 5 -4.096977452287817 2216 6 -4.567409929701437 2216 7 -2.3797711162291293 2216 8 -1.6897502474572619 2216 9 -1.3191260725845595 2216 10-11 -1.1713133314109925 2216 12-13 -1.090212906635351 2216 14-15 -1.301625991898625 2216 16-17 -1.203677972703872 2216 18-19 -1.2046946540782955 2216 20-21 -1.3015204956757955 2216 22-23 -1.3155062320582758 2216 24-25 -1.8142914708866726 2216 26-27 -1.8179973233629596 2216 28-29 -2.6132035659249553 2216 30-31 -2.120123554085545 2216 32-33 -1.9881034067239582 2216 34-35 -2.022744975783361 2216 36-37 -2.0722408619323005 2216 38-39 -2.1339893548332256 2216 40-41 -2.251712483557128 2216 42-43 -2.364474579380989 2216 44-45 -2.3556715061438354 2216 46-47 -2.4171169721590218 2216 48-49 -2.5071439370530513 2216 50-51 -2.565863337383341 2216 52-53 -2.6442179434591466 2216 54-55 -2.4067422550311974 2216 56-57 -2.5685951573992014 2216 58-59 -2.5827469968392833 2216 60-61 -2.7484311309396787 2216 62-63 -2.8633401599804458 2216 64-65 -2.9823045182247583 2216 66-67 -3.0642485982663814 2216 68-69 -3.1192230474221674 2216 70-71 -3.0201744865078197 2216 72-73 -3.0830952520467356 2216 74-75 -3.1827705108633495 2216 76-77 -3.2244456956633414 2216 78-79 -3.1640727786845524 2216 80-81 -3.2551435120460717 2216 82-83 -3.2412402851211617 2216 84-85 -3.0845900336757737 2216 86-87 -3.1858882408575653 2216 88-89 -3.083784780654902 2216 90-91 -3.208457646677914 2216 92-93 -3.2783111189394205 2216 94-95 -3.40848690589506 2216 96-97 -3.6694408834080185 2216 98-99 -3.8510677236096065 2216 100 -3.9546441686479668 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5781.0 3 2359.0 4 2832.0 5 3853.0 6 3192.0 7 2726.0 8 2940.0 9 3506.0 10 4345.0 11 14007.0 12 8874.0 13 13357.0 14 11488.0 15 11401.0 16 12565.0 17 14400.0 18 17022.0 19 22396.0 20 39234.0 21 53951.0 22 27338.0 23 18454.0 24 19247.0 25 20794.0 26 22324.0 27 25537.0 28 29394.0 29 34658.0 30 41844.0 31 52894.0 32 67723.0 33 92387.0 34 132761.0 35 248672.0 36 402811.0 37 787097.0 38 857515.0 39 284242.0 40 2997.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 13.980698395454617 46.5957918802851 22.33041588940677 17.093093834853516 2 16.163681024230474 44.367750264849874 21.696045357039857 17.772523353879794 3 16.869781609269367 19.953359513155917 17.52431617254348 45.65254270503124 4 40.75562745637714 21.7859684396212 19.735342318466106 17.723061785535553 5 16.405060491826827 43.789945535563064 20.38793515751218 19.41705881509793 6 43.82700214723397 18.506079734598256 21.11209813219017 16.554819985977606 7 17.99999298023527 19.792460655681126 44.98581130053426 17.221735063549346 8 43.11147561889464 20.558053746828676 19.38949691101103 16.940973723265664 9 42.986406810575744 21.17988790605683 21.393551995104882 14.440153288262545 10-11 14.880160916406886 46.974554522805164 20.661975513890653 17.483309046897293 12-13 14.724526882481534 20.015733047706906 33.93282611633271 31.326913953478847 14-15 15.512709291483032 33.539417171992525 19.447801583893842 31.5000719526306 16-17 29.70931344705002 19.17388193767246 33.260434770654705 17.85636984462281 18-19 16.523268472657136 20.43769403068456 33.053819951224334 29.98521754543396 20-21 30.191174517786035 33.33725757681231 20.788287405547603 15.683280499854046 22-23 28.444000964048254 20.005039609045674 34.00446223305866 17.546497193847408 24-25 15.245617673392536 33.177742177127634 20.572635515090283 31.00400463438955 26-27 15.740891330127013 30.484332192732488 36.438891661250324 17.335884815890175 28-29 37.35651910414482 19.325708708279628 26.402108176701805 16.91566401087374 30-31 26.490267395258577 28.10611100784046 27.12926003011928 18.274361566781685 32-33 16.934965159123198 28.487838964744945 27.244752516139297 27.33244335999256 34-35 23.980010160804312 28.737829452669683 27.261440888650547 20.02071949787546 36-37 25.379730346289502 21.680648077025193 34.449344736129554 18.49027684055575 38-39 32.65848903784551 22.279194294334665 27.751443655209044 17.31087301261078 40-41 16.446894075382946 37.11494581344475 27.705488737836877 18.732671373335435 42-43 16.235961169228823 22.03139936093265 35.32132673751555 26.411312732322976 44-45 16.480144898009627 29.76796164485099 26.530645376191192 27.22124808094819 46-47 24.93335164344193 21.34411699281913 34.15706884995699 19.565462513781938 48-49 17.921279162420305 22.149047625176532 34.01715374543821 25.912519466964955 50-51 25.546714491585032 29.377456520330586 27.312754519017467 17.76307446906691 52-53 24.163275298737016 22.404322036215962 34.06320806855753 19.369194596489496 54-55 17.003340730689953 30.004488727502064 25.721139888143075 27.27103065366491 56-57 17.479317243055704 30.399096841490064 32.1439864461871 19.977599469267133 58-59 33.033593221856144 22.658436392154844 24.962739690924458 19.345230695064554 60-61 24.526922937979563 30.4002260537805 24.87681195136509 20.196039056874845 62-63 17.594878783974437 29.939654134817133 24.195002128623873 28.270464952584557 64-65 24.16582649015476 30.10452024958252 24.560569279129286 21.169083981133436 66-67 25.626514439755276 23.10548047301416 31.557253574107076 19.710751513123487 68-69 32.258808495092424 23.667162984452787 25.52268756300557 18.55134095744922 70-71 16.964592854685733 37.47544339373176 25.628745650306904 19.931218101275597 72-73 16.659263875407294 23.559157711044268 32.54021821683358 27.241360196714858 74-75 16.980258115847917 30.59111571335802 24.337320322235286 28.091305848558772 76-77 24.975198597695147 22.857963981475184 31.21374399161074 20.953093429218924 78-79 18.641339785870397 23.60057945288391 30.980207974673508 26.77787278657219 80-81 25.74841471398158 30.369979050062803 24.85652130417308 19.025084931782533 82-83 24.440155710772288 24.028354396313606 31.19183973754705 20.339650155367057 84-85 17.562581848039144 30.798365920443633 24.20793328893027 27.431118942586956 86-87 17.762198035641962 30.92015859139343 30.8598245911439 20.4578187818207 88-89 32.039618581994354 23.386960763046645 24.72391895898611 19.84950169597289 90-91 24.246413903028856 30.579420124971275 24.585719444912108 20.588446527087765 92-93 17.732624428942056 30.218939004217415 23.981837311327634 28.066599255512898 94-95 23.877084082275456 30.77544431219837 23.76283049371532 21.584641111810853 96-97 25.304863066111583 24.082470987988007 30.177187396887135 20.435478549013276 98-99 31.61922149856239 24.754139388029223 24.35260057521912 19.274038538189263 100 17.56930896253254 37.019845522097924 24.805513064332445 20.605332451037093 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 46202.0 1 24895.0 2 2697.5 3 1478.0 4 1143.0 5 1085.0 6 1209.0 7 1546.5 8 1441.0 9 1257.0 10 1385.0 11 1965.5 12 17149.5 13 19249.0 14 6024.5 15 5658.5 16 6472.5 17 7296.0 18 8072.5 19 8907.0 20 9871.0 21 10962.5 22 12255.0 23 13604.5 24 14782.5 25 16232.5 26 18037.0 27 19923.0 28 21813.0 29 23804.5 30 25752.0 31 27980.5 32 30327.0 33 32911.5 34 36167.5 35 39086.0 36 41929.5 37 44798.5 38 46903.0 39 48169.5 40 49361.0 41 51182.0 42 52785.5 43 54260.5 44 56720.0 45 60878.5 46 70199.5 47 94994.0 48 124306.0 49 245645.0 50 676028.0 51 678744.5 52 226930.0 53 70892.0 54 43075.5 55 34960.5 56 29543.0 57 24134.0 58 20566.5 59 16802.0 60 12906.0 61 10170.0 62 8099.5 63 6280.0 64 4701.0 65 3612.0 66 2964.0 67 2402.5 68 1846.0 69 1474.0 70 1255.0 71 1000.5 72 788.0 73 604.0 74 422.0 75 336.5 76 258.0 77 168.5 78 107.0 79 70.0 80 42.0 81 23.5 82 13.0 83 6.5 84 4.0 85 2.5 86 3.0 87 3.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05849803943820822 2 0.0 3 0.0 4 3.2173921691014526E-4 5 0.010441900039720169 6 0.0029541509916295155 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 5.849803943820823E-5 16-17 3.509882366292494E-4 18-19 0.0 20-21 0.0 22-23 5.849803943820823E-5 24-25 0.003773123543764431 26-27 0.007370752969214236 28-29 0.0011553362789046126 30-31 0.00519170100014098 32-33 0.002603162755000266 34-35 0.025783010882390277 36-37 0.025373524606322816 38-39 0.022565618713288823 40-41 0.0023691705972474332 42-43 0.005440317667753366 44-45 0.006946642183287228 46-47 0.0012138343183428209 48-49 0.008950200034045858 50-51 0.009871544155197639 52-53 0.0019450598113204237 54-55 0.010368777490422408 56-57 0.007283005910056924 58-59 0.01172885690736075 60-61 0.007677867676264829 62-63 0.0012430833380619249 64-65 0.014317395152501465 66-67 0.0047383411944948665 68-69 0.0021498029493541523 70-71 0.008365219639663777 72-73 0.004752965704354418 74-75 0.008716207876293026 76-77 0.007619369636826621 78-79 0.015238739273653242 80-81 0.0085407137579784 82-83 0.011714232397501198 84-85 0.0068735196339894675 86-87 0.013088936324299092 88-89 0.01969921478081662 90-91 0.01791502457795127 92-93 0.017300795163850084 94-95 0.006054547081854552 96-97 0.009769172586180774 98-99 0.01662806771031069 100 0.016525696141293823 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3418918.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.02162050560288 #Duplication Level Percentage of deduplicated Percentage of total 1 87.9109213770776 28.150501626346713 2 6.951043549100223 4.451673572944127 3 1.5775700967437758 1.5154905287674927 4 0.7025647891705569 0.8998905223767388 5 0.4606338292629763 0.737512083635085 6 0.34660975377733627 0.6659403599398992 7 0.27944144648828523 0.6263717567089224 8 0.2226261740064456 0.5703080686918972 9 0.1916812523035048 0.5524149887371395 >10 1.2039306556325866 7.44758257967055 >50 0.07176311400529169 1.5767027298761394 >100 0.06379913790676386 4.20263695860204 >500 0.007882499521635873 1.8283025075950063 >1k 0.0038495927896361236 2.3650268258392693 >5k 0.001374854567727187 3.4729911181628292 >10k+ 0.004307877645545187 40.936653772106176 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG 407074 11.906515453134588 No Hit AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 140786 4.117852490173792 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 45080 1.3185458089372135 No Hit GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT 37323 1.0916611629761228 No Hit TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG 26709 0.7812120676775518 No Hit AGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTC 26665 0.7799251108099112 No Hit CCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATT 25334 0.7409946655637836 No Hit CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA 25227 0.7378650204538395 No Hit CCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGAT 24403 0.7137638282052977 No Hit CTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCG 23555 0.6889606594834974 No Hit TCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGA 22789 0.6665559103786637 No Hit TCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGT 22405 0.6553242868065277 No Hit TCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCA 22126 0.6471638103048977 No Hit TACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACT 22090 0.6461108455950099 No Hit ACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTC 21514 0.629263410236806 No Hit CCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTC 21484 0.6283859396452328 No Hit ACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGA 21474 0.6280934494480418 No Hit CGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAG 20993 0.6140246709631526 No Hit TGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCA 20973 0.6134396905687706 No Hit TCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACT 20388 0.5963290140330947 No Hit ATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTA 20045 0.586296600269442 No Hit ACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAG 19094 0.558480782516574 No Hit CTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGC 18664 0.5459037040373591 No Hit CAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTT 18092 0.5291732647580316 No Hit GTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCAC 17662 0.5165961862788169 No Hit AACGAGTGGAATCACTGCTGAGTACATGGTAACGAGTGGAATCACTGCTG 17649 0.5162159490224685 No Hit GAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGT 17157 0.5018254313206693 No Hit GATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGT 17107 0.500362980334714 No Hit TGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATG 17085 0.4997195019008938 No Hit ATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCC 16349 0.4781922233876332 No Hit GTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCAT 16065 0.46988550178740757 No Hit CGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAG 15257 0.4462522938543715 No Hit CTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACG 14811 0.43320723105965103 No Hit TTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTAC 14438 0.4222973467044252 No Hit CTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGT 14187 0.4149558427549301 No Hit TGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTG 13943 0.40781908194346866 No Hit ATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGG 13818 0.40416295447858064 No Hit TGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGA 13732 0.40164753878273773 No Hit ATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCAC 12526 0.36637322100149816 No Hit ACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATC 12016 0.35145622094475504 No Hit CAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCC 11886 0.3476538483812715 No Hit GCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCC 11685 0.3417747954177316 No Hit TGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACA 11378 0.33279534636396657 No Hit ACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAAC 11106 0.3248396130003703 No Hit GGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACAT 10357 0.3029320972307613 No Hit TACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAAT 10168 0.2974040325038506 No Hit GAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATG 10104 0.29553209524182794 No Hit AGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGA 9883 0.28906806188390594 No Hit GGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGC 9586 0.28038110302733205 No Hit TCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGA 9489 0.27754394811457894 No Hit GGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTG 8987 0.2628609402155887 No Hit TGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACT 8890 0.2600237853028356 No Hit AACGAGTGGAATCACTGCTGAGTACATGGAAACGAGTGGAATCACTGCTG 8588 0.25119058134766614 No Hit AGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCA 8187 0.23946172444030536 No Hit CACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAA 8154 0.23849650678957496 No Hit GAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGG 8057 0.23565935187682185 No Hit GAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCT 7603 0.22238029692434857 No Hit GTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTAC 7113 0.20804827726198755 No Hit TACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG 7076 0.2069660635323807 No Hit GCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAG 5854 0.17122376143563547 No Hit GTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAA 5589 0.1634727712100729 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGTAACGAGTGGAATCACTGCT 5274 0.1542593299985551 No Hit CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 4934 0.1443146632940597 No Hit CATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTC 4819 0.14095102602636272 No Hit ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 3471 0.10152334744501038 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0029541509916295155 0.0 0.0 0.0 0.0 2 0.0029541509916295155 0.0 0.0 0.0 0.0 3 0.0029541509916295155 0.0 0.0 0.0 0.0 4 0.0029541509916295155 0.0 0.0 2.9249019719104114E-5 0.0 5 0.0029541509916295155 2.9249019719104114E-5 0.0 2.9249019719104114E-5 0.0 6 0.0029541509916295155 2.9249019719104114E-5 0.0 2.9249019719104114E-5 0.0 7 0.0029541509916295155 2.9249019719104114E-5 0.0 2.9249019719104114E-5 0.0 8 0.0029834000113486197 5.849803943820823E-5 0.0 2.9249019719104114E-5 0.0 9 0.0030418980507868278 5.849803943820823E-5 0.0 2.9249019719104114E-5 0.0 10-11 0.0032320166789610045 5.849803943820823E-5 0.0 2.9249019719104114E-5 0.0 12-13 0.0033928862874160774 5.849803943820823E-5 0.0 2.9249019719104114E-5 0.0 14-15 0.004065613740955472 5.849803943820823E-5 0.0 2.9249019719104114E-5 0.0 16-17 0.0047383411944948665 5.849803943820823E-5 0.0 2.9249019719104114E-5 0.0 18-19 0.005440317667753365 5.849803943820823E-5 0.0 2.9249019719104114E-5 0.0 20-21 0.006858895124129915 5.849803943820823E-5 0.0 2.9249019719104114E-5 0.0 22-23 0.008877077484748098 5.849803943820823E-5 0.0 2.9249019719104114E-5 0.0 24-25 0.01172885690736075 7.312254929776028E-5 0.0 2.9249019719104114E-5 0.0 26-27 0.014522138290535194 8.774705915731235E-5 0.0 2.9249019719104114E-5 0.0 28-29 0.01763715889061978 8.774705915731235E-5 0.0 2.9249019719104114E-5 0.0 30-31 0.02138103341466511 8.774705915731235E-5 0.0 2.9249019719104114E-5 0.0 32-33 0.026792102062699368 8.774705915731235E-5 0.0 2.9249019719104114E-5 0.0 34-35 0.034908705034750756 8.774705915731235E-5 0.0 2.9249019719104114E-5 1.4624509859552057E-5 36-37 0.041767600158880674 8.774705915731235E-5 0.0 2.9249019719104114E-5 2.9249019719104114E-5 38-39 0.049182226657673564 8.774705915731235E-5 0.0 2.9249019719104114E-5 2.9249019719104114E-5 40-41 0.05764981786635421 8.774705915731235E-5 0.0 2.9249019719104114E-5 2.9249019719104114E-5 42-43 0.0668340100581529 8.774705915731235E-5 0.0 2.9249019719104114E-5 2.9249019719104114E-5 44-45 0.07805100912042932 8.774705915731235E-5 0.0 2.9249019719104114E-5 2.9249019719104114E-5 46-47 0.08789330425590786 8.774705915731235E-5 0.0 2.9249019719104114E-5 2.9249019719104114E-5 48-49 0.09653638958290313 8.774705915731235E-5 0.0 2.9249019719104114E-5 2.9249019719104114E-5 50-51 0.10955220335790446 8.774705915731235E-5 0.0 2.9249019719104114E-5 2.9249019719104114E-5 52-53 0.1237233534118104 8.774705915731235E-5 0.0 2.9249019719104114E-5 2.9249019719104114E-5 54-55 0.13982493876717722 8.774705915731235E-5 0.0 2.9249019719104114E-5 2.9249019719104114E-5 56-57 0.15709648491130818 8.774705915731235E-5 0.0 2.9249019719104114E-5 2.9249019719104114E-5 58-59 0.1720134849680513 8.774705915731235E-5 0.0 2.9249019719104114E-5 2.9249019719104114E-5 60-61 0.19049886543052508 8.774705915731235E-5 0.0 2.9249019719104114E-5 2.9249019719104114E-5 62-63 0.21653049298052776 8.774705915731235E-5 0.0 2.9249019719104114E-5 2.9249019719104114E-5 64-65 0.2483826754546321 8.774705915731235E-5 0.0 2.9249019719104114E-5 2.9249019719104114E-5 66-67 0.2769297187004778 8.774705915731235E-5 0.0 2.9249019719104114E-5 2.9249019719104114E-5 68-69 0.30636885704775607 8.774705915731235E-5 0.0 2.9249019719104114E-5 2.9249019719104114E-5 70-71 0.34171629737829334 8.774705915731235E-5 0.0 2.9249019719104114E-5 2.9249019719104114E-5 72-73 0.37678587202149916 8.774705915731235E-5 0.0 2.9249019719104114E-5 2.9249019719104114E-5 74-75 0.4149412182450705 8.774705915731235E-5 0.0 2.9249019719104114E-5 2.9249019719104114E-5 76-77 0.44987917229954033 8.774705915731235E-5 0.0 2.9249019719104114E-5 2.9249019719104114E-5 78-79 0.4873032930301341 8.774705915731235E-5 0.0 2.9249019719104114E-5 2.9249019719104114E-5 80-81 0.5417649677471059 8.774705915731235E-5 0.0 2.9249019719104114E-5 2.9249019719104114E-5 82-83 0.6025151817036851 8.774705915731235E-5 0.0 2.9249019719104114E-5 2.9249019719104114E-5 84-85 0.6725080858915014 8.774705915731235E-5 0.0 2.9249019719104114E-5 2.9249019719104114E-5 86-87 0.7468737185273235 8.774705915731235E-5 0.0 2.9249019719104114E-5 2.9249019719104114E-5 88 0.8091741305290153 8.774705915731235E-5 0.0 2.9249019719104114E-5 2.9249019719104114E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGAGT 2285 0.0 72.218094 1 ACGTGTG 1485 0.0 58.54023 2 AACGTGT 1360 0.0 58.07584 1 CGTGTGG 1670 0.0 57.401447 3 TCGAGTG 1040 0.0 52.864197 2 ATCGAGT 925 0.0 52.85873 1 GTGTGGA 2315 0.0 43.438206 4 ATCTGCG 625 0.0 38.352016 94 TGAGTGG 2085 0.0 38.08814 3 CGATTGG 765 0.0 38.08372 3 ATGAGTG 1970 0.0 37.210667 2 TACGTGT 230 0.0 36.79339 1 ACGATTG 730 0.0 36.691135 2 AATGAGT 1820 0.0 36.422764 1 TGTGGAA 2800 0.0 35.91409 5 AGTACTT 25140 0.0 34.759872 20-21 GTACTTT 23805 0.0 34.07474 22-23 GAGTACT 25265 0.0 33.85317 20-21 TACTTTT 24515 0.0 33.83551 22-23 ATCGTGT 185 0.0 33.03671 1 >>END_MODULE