##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2132712_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3668553 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.92855548222964 33.0 31.0 34.0 25.0 34.0 2 31.653654724355896 34.0 31.0 34.0 28.0 34.0 3 31.86031249923335 34.0 31.0 34.0 28.0 34.0 4 35.78840403832247 37.0 35.0 37.0 35.0 37.0 5 35.448073123108756 37.0 35.0 37.0 33.0 37.0 6 35.801518473359934 37.0 36.0 37.0 35.0 37.0 7 35.99269902874512 37.0 35.0 37.0 35.0 37.0 8 36.15048358303669 37.0 37.0 37.0 35.0 37.0 9 38.075357504716436 39.0 39.0 39.0 37.0 39.0 10-11 38.162541743297695 39.0 39.0 39.0 37.0 39.0 12-13 38.163025994172635 39.0 39.0 39.0 37.0 39.0 14-15 39.73787144413615 41.0 40.0 41.0 38.0 41.0 16-17 39.67983602799251 41.0 40.0 41.0 37.5 41.0 18-19 39.66877471853344 41.0 40.0 41.0 37.0 41.0 20-21 39.56343413874626 41.0 40.0 41.0 37.0 41.0 22-23 39.44397259627979 41.0 39.5 41.0 37.0 41.0 24-25 39.09532750378692 41.0 39.0 41.0 35.5 41.0 26-27 39.14838343619405 41.0 39.0 41.0 36.0 41.0 28-29 39.060438134599664 41.0 39.0 41.0 36.0 41.0 30-31 38.87148638713956 41.0 39.0 41.0 35.0 41.0 32-33 38.74778270887731 41.0 39.0 41.0 35.0 41.0 34-35 38.5977961883064 41.0 39.0 41.0 35.0 41.0 36-37 38.27020558241901 40.5 38.5 41.0 34.5 41.0 38-39 38.010622444326145 40.0 38.0 41.0 34.0 41.0 40-41 37.97379729828082 40.0 38.0 41.0 34.0 41.0 42-43 37.919944321371396 40.0 38.0 41.0 34.0 41.0 44-45 37.75321277898943 40.0 37.0 41.0 33.0 41.0 46-47 37.672847168897384 40.0 37.0 41.0 33.0 41.0 48-49 37.51194176559532 40.0 37.0 41.0 33.0 41.0 50-51 37.257081334248134 40.0 37.0 41.0 33.0 41.0 52-53 36.75536444478245 40.0 36.0 41.0 31.5 41.0 54-55 35.86428013988076 39.0 35.0 41.0 28.0 41.0 56-57 35.644482715664736 39.0 35.5 41.0 28.0 41.0 58-59 35.64163268187757 40.0 36.0 41.0 27.0 41.0 60-61 35.374608462791734 39.0 35.0 41.0 26.0 41.0 62-63 35.192693549745634 39.0 35.0 41.0 26.0 41.0 64-65 34.740231502720555 39.0 35.0 40.5 24.5 41.0 66-67 34.08043879971204 37.5 34.5 40.0 21.5 41.0 68-69 33.88450432636519 37.0 34.0 40.0 23.0 41.0 70-71 33.65547655983163 37.0 34.0 39.0 22.5 41.0 72-73 33.309915653392494 36.5 34.0 39.0 22.0 41.0 74-75 32.903258042067264 36.0 34.0 39.0 20.5 40.5 76-77 32.49127053636679 35.5 33.5 38.0 19.5 40.0 78-79 32.08449693925643 35.0 33.0 37.0 18.0 39.0 80-81 31.662300912648668 35.0 32.5 37.0 15.0 39.0 82-83 31.03960158133193 35.0 31.5 36.0 9.5 38.0 84-85 30.590777344636972 35.0 31.0 36.0 7.5 37.0 86-87 30.644542003345734 35.0 31.5 35.5 7.0 37.0 88-89 30.773201041391523 35.0 32.5 35.0 2.0 36.5 90-91 30.54214659022236 35.0 32.0 35.0 2.0 36.0 92-93 30.4123384615133 35.0 32.0 35.0 2.0 36.0 94-95 30.074410264755613 35.0 32.0 35.0 2.0 36.0 96-97 29.603260059211358 35.0 31.0 35.0 2.0 35.0 98-99 29.529146232860747 35.0 31.0 35.0 2.0 35.0 100 29.597102726878962 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.8860037881168132 1101 2 1.4194563362834884 1101 3 1.3132197733638478 1101 4 0.7216571066315041 1101 5 0.9777546976932499 1101 6 0.6681453284606178 1101 7 0.47468041129649663 1101 8 0.37100500342644693 1101 9 0.3614671470330464 1101 10-11 0.23627503354641988 1101 12-13 0.3064595417818552 1101 14-15 0.3472568947957555 1101 16-17 0.3711818265768869 1101 18-19 0.3383051514602329 1101 20-21 0.4423292357026938 1101 22-23 0.4397456575503611 1101 24-25 0.4227346706561761 1101 26-27 0.434337297820953 1101 28-29 0.47872398267544725 1101 30-31 0.5404454399284191 1101 32-33 0.5367090354727111 1101 34-35 0.49897613505911664 1101 36-37 0.49691810373268197 1101 38-39 0.5436144484480607 1101 40-41 0.564356604295547 1101 42-43 0.5517529949542919 1101 44-45 0.6508412344037069 1101 46-47 0.6022707784157362 1101 48-49 0.5367535603972868 1101 50-51 0.5468749456876765 1101 52-53 0.6464055848142465 1101 54-55 0.46114859000001474 1101 56-57 0.5403879231978337 1101 58-59 0.6204503929567622 1101 60-61 0.6782857980056107 1101 62-63 0.7122572756969205 1101 64-65 0.734005688555186 1101 66-67 0.7677339369518847 1101 68-69 0.8551938823706209 1101 70-71 0.7113923857386766 1101 72-73 0.785560612558001 1101 74-75 0.7524696789768583 1101 76-77 0.8110021518057522 1101 78-79 0.7886667805128909 1101 80-81 0.7888679387338762 1101 82-83 0.7348533414041256 1101 84-85 0.6537512000892249 1101 86-87 0.6855182986076933 1101 88-89 0.6549899304432572 1101 90-91 0.6895439305964608 1101 92-93 0.769760640422902 1101 94-95 0.833059510511653 1101 96-97 0.7782279769651517 1101 98-99 0.7598320063369677 1101 100 0.8701187298407014 1102 1 1.3133251096906768 1102 2 1.172612132016738 1102 3 1.1633492736294784 1102 4 0.5655940845165901 1102 5 0.8833773865602694 1102 6 0.6026035984858567 1102 7 0.30394451987133664 1102 8 0.27787530990097764 1102 9 0.2629182535062782 1102 10-11 0.2383485135254304 1102 12-13 0.1965502647434647 1102 14-15 0.3476037719512277 1102 16-17 0.3349526414299149 1102 18-19 0.27782046144012185 1102 20-21 0.3468542652779192 1102 22-23 0.3944163395473623 1102 24-25 0.34334059686002405 1102 26-27 0.320420466667791 1102 28-29 0.46791956486869424 1102 30-31 0.4309480934102936 1102 32-33 0.41396180640642655 1102 34-35 0.45311143069411486 1102 36-37 0.4576390114555551 1102 38-39 0.3830128321812438 1102 40-41 0.3140666741035858 1102 42-43 0.35449479818989715 1102 44-45 0.4009844811852048 1102 46-47 0.29298480479449296 1102 48-49 0.3276252471837324 1102 50-51 0.367403487093398 1102 52-53 0.2866732739521609 1102 54-55 0.1405790718477391 1102 56-57 0.1663935616951946 1102 58-59 0.10614874022124354 1102 60-61 0.14684104727441394 1102 62-63 0.13464171803715885 1102 64-65 0.12927161086149397 1102 66-67 0.09725617679300314 1102 68-69 0.11157368081435237 1102 70-71 0.10298513792861996 1102 72-73 0.21197646326113784 1102 74-75 0.2816325687209016 1102 76-77 0.29136852879828723 1102 78-79 0.27355414398775224 1102 80-81 0.3041182223568448 1102 82-83 0.310034997568188 1102 84-85 0.31615777432000414 1102 86-87 0.2615790444613033 1102 88-89 0.2807887040923198 1102 90-91 0.3112774147641133 1102 92-93 0.36581607769552704 1102 94-95 0.4010669611232487 1102 96-97 0.41621610967886724 1102 98-99 0.5120732544559203 1102 100 0.5147926018379962 1103 1 1.8717863166913453 1103 2 1.3462476895324187 1103 3 1.2379802237192052 1103 4 0.6330843035738667 1103 5 0.9328596842422172 1103 6 0.6008582232490767 1103 7 0.39973647429108894 1103 8 0.29492009769678873 1103 9 0.2825641759771642 1103 10-11 0.1898869519875035 1103 12-13 0.19087394791027634 1103 14-15 0.284472354729445 1103 16-17 0.3193060161181407 1103 18-19 0.2746896052443262 1103 20-21 0.3809037119270684 1103 22-23 0.40843207398881987 1103 24-25 0.3041904694214139 1103 26-27 0.35723481693345605 1103 28-29 0.4254330859846931 1103 30-31 0.4491383092728327 1103 32-33 0.3877979891544072 1103 34-35 0.40001630094516116 1103 36-37 0.48980641070268405 1103 38-39 0.4449560820645573 1103 40-41 0.4140708817985228 1103 42-43 0.5056993482665249 1103 44-45 0.5340679501647543 1103 46-47 0.5161704116726682 1103 48-49 0.4394235653524845 1103 50-51 0.45233290690680406 1103 52-53 0.42418528341479345 1103 54-55 0.32961759797012036 1103 56-57 0.44525719965265154 1103 58-59 0.4506697175285197 1103 60-61 0.5138607447411445 1103 62-63 0.40446142220503845 1103 64-65 0.3329632203281818 1103 66-67 0.4617302772618501 1103 68-69 0.45221191021632023 1103 70-71 0.40875034231739704 1103 72-73 0.4045152656109039 1103 74-75 0.40038585598258436 1103 76-77 0.4845555304494198 1103 78-79 0.4682617598376666 1103 80-81 0.566417879585547 1103 82-83 0.5753234818084252 1103 84-85 0.5086430454397117 1103 86-87 0.5254654702036667 1103 88-89 0.5415574421656473 1103 90-91 0.4623200270258039 1103 92-93 0.5078589369644959 1103 94-95 0.579547835201744 1103 96-97 0.5785215135279742 1103 98-99 0.6179392097624081 1103 100 0.5590539293034134 1104 1 1.8614588863273518 1104 2 1.3462446823458833 1104 3 1.2382184304827533 1104 4 0.6277463595218933 1104 5 0.930708530942951 1104 6 0.6190372297114806 1104 7 0.3768915171981888 1104 8 0.3410407913539828 1104 9 0.3096185176046049 1104 10-11 0.24895454222631486 1104 12-13 0.20838336670043134 1104 14-15 0.2999839303489651 1104 16-17 0.2856723074515912 1104 18-19 0.27827930260821887 1104 20-21 0.3000561033226248 1104 22-23 0.3011848258558274 1104 24-25 0.3343157123503957 1104 26-27 0.35721461239891283 1104 28-29 0.36474561833309593 1104 30-31 0.3652130707869645 1104 32-33 0.32795800305362377 1104 34-35 0.33583418181915903 1104 36-37 0.3834931195283531 1104 38-39 0.4280251143996665 1104 40-41 0.42200447890972725 1104 42-43 0.35990508094892704 1104 44-45 0.32890945245140557 1104 46-47 0.32773540454807915 1104 48-49 0.33740628447986865 1104 50-51 0.29426572869014933 1104 52-53 0.3914083409874962 1104 54-55 0.40335732650162726 1104 56-57 0.44079242980146915 1104 58-59 0.33292659899564114 1104 60-61 0.29999012691204285 1104 62-63 0.36840807486623817 1104 64-65 0.39675369099761326 1104 66-67 0.38148066649516466 1104 68-69 0.39689405604990924 1104 70-71 0.4019815413213834 1104 72-73 0.39093652154807046 1104 74-75 0.28741595066497183 1104 76-77 0.4046789235827859 1104 78-79 0.39912340260578816 1104 80-81 0.39230510580518185 1104 82-83 0.4809111125585446 1104 84-85 0.5546263294670872 1104 86-87 0.4484707817596565 1104 88-89 0.43175275103734734 1104 90-91 0.4661516713414926 1104 92-93 0.5059379274098745 1104 94-95 0.5165303430366848 1104 96-97 0.5586272454548116 1104 98-99 0.5625022898040548 1104 100 0.5822834303747051 1105 1 1.5613670622156839 1105 2 1.2833252581868422 1105 3 1.2026736213262765 1105 4 0.6107954572435048 1105 5 0.9907111892019529 1105 6 0.6544724369173522 1105 7 0.38707915573761653 1105 8 0.3086445406702083 1105 9 0.28677666601011254 1105 10-11 0.23734862882963625 1105 12-13 0.19014852971859852 1105 14-15 0.2230941182583024 1105 16-17 0.21557205258954326 1105 18-19 0.25401310139060485 1105 20-21 0.29819793856648147 1105 22-23 0.3246501643395874 1105 24-25 0.2917974928101188 1105 26-27 0.2653605843387723 1105 28-29 0.32029809340497195 1105 30-31 0.3063627334032475 1105 32-33 0.3432566605010976 1105 34-35 0.3403171394660305 1105 36-37 0.31989319350876144 1105 38-39 0.3248446670656122 1105 40-41 0.2731229091785181 1105 42-43 0.34334237816701574 1105 44-45 0.3655668220426449 1105 46-47 0.4244817416472344 1105 48-49 0.40621477331379197 1105 50-51 0.39752337312428665 1105 52-53 0.3408501688098937 1105 54-55 0.2847443755984713 1105 56-57 0.377217516447395 1105 58-59 0.3833729735241391 1105 60-61 0.4146839938430489 1105 62-63 0.5450863826384662 1105 64-65 0.4077651007253422 1105 66-67 0.4991522482573387 1105 68-69 0.49018831595239476 1105 70-71 0.46696031111250136 1105 72-73 0.47509815774746755 1105 74-75 0.38537092830618036 1105 76-77 0.484215723416618 1105 78-79 0.4364082113436112 1105 80-81 0.4129063751531241 1105 82-83 0.5276672863870182 1105 84-85 0.5409151803394892 1105 86-87 0.550537680594605 1105 88-89 0.46859027769129824 1105 90-91 0.50366324084289 1105 92-93 0.5540935845758526 1105 94-95 0.6140239678260642 1105 96-97 0.6741573075681941 1105 98-99 0.6651637106054267 1105 100 0.6890805225087036 1106 1 1.6433859534340947 1106 2 1.2896909500283797 1106 3 1.1692700432687175 1106 4 0.6520544975183427 1106 5 0.9822974175309511 1106 6 0.6629758103087084 1106 7 0.4021640023286963 1106 8 0.32638610662472445 1106 9 0.324908861439404 1106 10-11 0.27766436998047794 1106 12-13 0.26465009179416654 1106 14-15 0.32451029329696723 1106 16-17 0.3431694391682285 1106 18-19 0.3613692892116873 1106 20-21 0.37480960697886445 1106 22-23 0.35354267856952504 1106 24-25 0.27718021975341145 1106 26-27 0.3078124475158077 1106 28-29 0.4361035675029825 1106 30-31 0.48660269021295477 1106 32-33 0.47720088286307316 1106 34-35 0.40467761569350813 1106 36-37 0.4567184939722466 1106 38-39 0.5023001653504267 1106 40-41 0.4502670698400735 1106 42-43 0.5234539578283304 1106 44-45 0.5072211873760821 1106 46-47 0.5320331534697758 1106 48-49 0.5540040209939292 1106 50-51 0.4916867709349475 1106 52-53 0.46742294837434883 1106 54-55 0.46849503272500925 1106 56-57 0.5485114787091945 1106 58-59 0.58405906467177 1106 60-61 0.5364653497666083 1106 62-63 0.5837766748591662 1106 64-65 0.5737602979597156 1106 66-67 0.5072419225664291 1106 68-69 0.5595947017195613 1106 70-71 0.6120442106474187 1106 72-73 0.6403680347265777 1106 74-75 0.6232262090516159 1106 76-77 0.6761200662078508 1106 78-79 0.6198010995756746 1106 80-81 0.655002993028436 1106 82-83 0.6607180434932012 1106 84-85 0.6797142705984811 1106 86-87 0.6740522343525193 1106 88-89 0.6221961940134975 1106 90-91 0.6332152776090822 1106 92-93 0.681505813169121 1106 94-95 0.7146425174237336 1106 96-97 0.6855764939051809 1106 98-99 0.7278496129089298 1106 100 0.7755562486654313 1107 1 1.5507219033115014 1107 2 1.225433684363331 1107 3 1.1854082979317582 1107 4 0.6348271759733777 1107 5 0.9602980345528707 1107 6 0.6280757980146632 1107 7 0.39227483431084664 1107 8 0.31403986918262916 1107 9 0.31447573988840105 1107 10-11 0.20229520066362738 1107 12-13 0.131455704373991 1107 14-15 0.1842343615590778 1107 16-17 0.21123954074488438 1107 18-19 0.20022450478661824 1107 20-21 0.25922180081654034 1107 22-23 0.2521220203973513 1107 24-25 0.2523795920611889 1107 26-27 0.23382316885154353 1107 28-29 0.3067977519554219 1107 30-31 0.28087720233650515 1107 32-33 0.32242319632412375 1107 34-35 0.3388589482288893 1107 36-37 0.3488968133066024 1107 38-39 0.31711704205969937 1107 40-41 0.3663926825126822 1107 42-43 0.3705737296694025 1107 44-45 0.42915225721321804 1107 46-47 0.38155838293931055 1107 48-49 0.42012563230260014 1107 50-51 0.46309035662096676 1107 52-53 0.3462706367458068 1107 54-55 0.1699831341763982 1107 56-57 0.22152175699535803 1107 58-59 0.24966102058485262 1107 60-61 0.30437145341236516 1107 62-63 0.3559723566335862 1107 64-65 0.3830054203550617 1107 66-67 0.35250395971300463 1107 68-69 0.42710722192934725 1107 70-71 0.3982032028140239 1107 72-73 0.3559890696068777 1107 74-75 0.3851960863199295 1107 76-77 0.31791796601368816 1107 78-79 0.35408008858757967 1107 80-81 0.41558080157999555 1107 82-83 0.4539904749074992 1107 84-85 0.3862382915401099 1107 86-87 0.3693814248244003 1107 88-89 0.3465338233039468 1107 90-91 0.36553098870364664 1107 92-93 0.42177727961681555 1107 94-95 0.4282605507011681 1107 96-97 0.4397611087188089 1107 98-99 0.4393657703005509 1107 100 0.40512911176311306 1108 1 0.7441723557673967 1108 2 0.9211470565323623 1108 3 0.9992461601678855 1108 4 0.5560208085421223 1108 5 0.8845393757871562 1108 6 0.5484103590607532 1108 7 0.3310695946650313 1108 8 0.2644576555581466 1108 9 0.22128387117006554 1108 10-11 0.17670316144710085 1108 12-13 0.1692233262564855 1108 14-15 0.17218513868313323 1108 16-17 0.19778167202608188 1108 18-19 0.16360932438565357 1108 20-21 0.1942947175146017 1108 22-23 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-1.074652265031002 2215 80-81 -1.1398263879970862 2215 82-83 -1.2117032903638147 2215 84-85 -1.1119473621523213 2215 86-87 -1.1442996144832058 2215 88-89 -1.099955912725882 2215 90-91 -1.1006182446005717 2215 92-93 -1.1220591167661773 2215 94-95 -1.2054320390493345 2215 96-97 -1.291963417734209 2215 98-99 -1.2848489907435976 2215 100 -1.2749757089689808 2216 1 -6.245567633063796 2216 2 -6.180252956648214 2216 3 -5.661320674030861 2216 4 -3.058802290766586 2216 5 -4.027239440250135 2216 6 -4.504663802498442 2216 7 -2.259857839319352 2216 8 -1.4714188077460832 2216 9 -1.22285832644841 2216 10-11 -1.0740559080041407 2216 12-13 -0.935527619796801 2216 14-15 -1.1785378576089585 2216 16-17 -1.1406123925518017 2216 18-19 -1.1530670473482516 2216 20-21 -1.1878883329565966 2216 22-23 -1.2257420074567875 2216 24-25 -1.677451450636262 2216 26-27 -1.574926693710303 2216 28-29 -2.267761163199424 2216 30-31 -1.8501501020431874 2216 32-33 -1.7215658086236516 2216 34-35 -1.697508286515479 2216 36-37 -1.7739039929972265 2216 38-39 -1.8044955656001846 2216 40-41 -1.7834882044215306 2216 42-43 -1.8557704154652939 2216 44-45 -1.8582943251580488 2216 46-47 -1.9569177028221034 2216 48-49 -1.954635591813222 2216 50-51 -2.0699285182186884 2216 52-53 -2.0816142817075587 2216 54-55 -1.814792383507303 2216 56-57 -1.9399149298289018 2216 58-59 -1.931118895119745 2216 60-61 -2.053217698136102 2216 62-63 -2.1300448689482323 2216 64-65 -2.0880753932788068 2216 66-67 -2.3066215671332486 2216 68-69 -2.3340230694395316 2216 70-71 -2.241881825589857 2216 72-73 -2.330251722036884 2216 74-75 -2.386307670322914 2216 76-77 -2.4511214329234505 2216 78-79 -2.439174080369721 2216 80-81 -2.529470325412859 2216 82-83 -2.537466369512508 2216 84-85 -2.4064631927919393 2216 86-87 -2.416715698136933 2216 88-89 -2.406308397696602 2216 90-91 -2.5387827367865476 2216 92-93 -2.6534581977517036 2216 94-95 -2.7815895500868137 2216 96-97 -2.947849666432372 2216 98-99 -3.054303278627433 2216 100 -2.9801404502673066 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3976.0 3 1289.0 4 1580.0 5 2025.0 6 1812.0 7 1793.0 8 1950.0 9 2502.0 10 3422.0 11 11007.0 12 7245.0 13 11147.0 14 9523.0 15 9906.0 16 10987.0 17 12701.0 18 15694.0 19 22610.0 20 44920.0 21 66645.0 22 32302.0 23 20615.0 24 20212.0 25 21887.0 26 22711.0 27 24722.0 28 28893.0 29 34021.0 30 41242.0 31 51237.0 32 66547.0 33 90493.0 34 130822.0 35 229130.0 36 399984.0 37 806797.0 38 992980.0 39 407950.0 40 3274.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 14.218230901257353 45.084250394994044 23.564827706776278 17.13269099697233 2 16.767510241776527 42.70621141360095 22.8115008833183 17.714777461304227 3 17.58552148095488 21.17301322156535 18.60715072322813 42.63431457425164 4 38.62983019393557 22.464971059315726 20.934361317778365 17.97083742897034 5 17.061855951147702 41.972057139741565 21.404912491140067 19.561174417970665 6 41.490717396536866 19.61167541540492 21.953949389523704 16.94365779853451 7 18.35233128702243 20.466707173100676 43.50922557204434 17.67173596783255 8 40.713763710105866 20.966359215745282 20.898485043012872 17.421392031135984 9 40.62285593257069 21.849486704976048 22.158409596372195 15.369247766081068 10-11 15.802988262674683 44.92929501086668 21.63701873736048 17.630697989098152 12-13 15.638577390049974 20.98556297264889 33.484496475858464 29.891363161442676 14-15 16.23861811267019 32.92233057874669 20.84270125401112 29.996350054572 16-17 28.75719137973497 20.351098658690383 33.04374504145585 17.8479649201188 18-19 16.941039150858664 21.230864049122363 32.88672672849486 28.941370071524116 20-21 29.074924091324288 32.83654345459913 21.688755757379 16.39977669669758 22-23 27.848130249772968 20.948894952135742 33.515830430620916 17.687144367470374 24-25 16.099479597498696 32.626160567362895 21.598305315067655 29.67605452007075 26-27 16.45325637095908 29.619841528168738 36.36953334609134 17.557368754780846 28-29 34.33862794431526 20.5050415563812 27.91738335182404 17.238947147479493 30-31 25.85977886737099 27.151438872307825 28.583448405771385 18.405333854549802 32-33 17.984540647721563 27.835652517855593 28.994367934874017 25.185438899548828 34-35 23.01432264596021 27.860606309304202 29.12301792174602 20.002053122989565 36-37 23.974523949780256 22.80056870955037 34.38491068345348 18.83999665721589 38-39 29.255036201210842 23.19212748061112 29.519930092333198 18.03290622584484 40-41 17.55332604600828 34.077626367395666 29.330892715491746 19.0381548711043 42-43 17.391371313431563 23.07408800208596 34.87343776131587 24.66110292316661 44-45 17.63743260141172 28.639837264730232 28.54462938988722 25.178100743970827 46-47 23.793813544964863 22.55842248953902 34.03716954096815 19.610594424527967 48-49 18.543445254204922 23.277906533434436 33.9202964698384 24.25835174252225 50-51 24.151705122592062 28.4694570771025 28.97724267770792 18.401595122597517 52-53 23.22223444342268 23.420326927561582 33.822117746859504 19.535320882156235 54-55 18.089419258173635 29.050286030834187 27.553640323041456 25.306654387950715 56-57 18.539719665976293 29.48442774280474 31.803579448532393 20.17227314268657 58-59 29.843483599473736 24.06092068021877 26.40193298245365 19.69366273785384 60-61 23.695042035488395 29.648953000863216 26.325355866028925 20.330649097619464 62-63 18.623106115330497 29.50221315528505 25.77315962467678 26.101521104707675 64-65 23.41448772706039 29.47366168313705 26.10544105252669 21.006409537275875 66-67 24.41057431947091 24.383695816031093 31.199400058718354 20.00632980577964 68-69 29.20940527699199 24.833537664518406 26.79627556155866 19.16078149693094 70-71 18.176961985874108 34.83599781202289 26.80269236226696 20.184347839836043 72-73 17.871377505443164 24.69748768047219 31.890996065547494 25.540138748537156 74-75 18.225050593222313 29.737692185751243 26.016481797685177 26.02077542334127 76-77 24.06735101203577 24.159151666539678 30.998021004153316 20.77547631727124 78-79 19.30899221249346 24.864636372422446 30.735630313110136 25.090741101973958 80-81 24.517618367324964 29.71619975456838 26.15701871596708 19.609163162139577 82-83 23.618045860537315 25.135484898126315 30.783908164425327 20.46256107691104 84-85 18.62415005069096 30.105127515227053 25.682842248481386 25.587880185600604 86-87 18.78142669421983 30.230026226397605 30.424924892177334 20.563622187205226 88-89 29.136001834334863 24.841959701069943 25.902114188163004 20.11992427643219 90-91 23.553299575422432 30.03884259058568 25.786973459607875 20.620884374384012 92-93 18.85034018520263 29.846315161268823 25.25783967708204 26.045504976446516 94-95 23.286991590444366 30.204232065461994 25.157122034104372 21.351654309989268 96-97 24.178702512653093 25.368217896940294 29.86616367351117 20.58691591689545 98-99 28.819851408608333 25.870134253530143 25.46674387682798 19.84327046103354 100 18.736460479731818 34.747514017305505 25.786825723030148 20.729199779932532 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 23316.0 1 12647.0 2 1569.5 3 1001.0 4 837.5 5 794.5 6 865.0 7 1125.5 8 1094.5 9 990.0 10 1047.0 11 1464.0 12 13131.0 13 15595.0 14 6698.5 15 7089.0 16 8201.0 17 9432.5 18 10572.0 19 11680.5 20 12990.0 21 14390.0 22 16118.0 23 18439.5 24 21112.5 25 23927.5 26 27406.0 27 30976.0 28 33967.0 29 36798.0 30 39876.0 31 43576.0 32 47772.0 33 52462.0 34 57206.0 35 61114.0 36 64904.5 37 68331.5 38 70062.0 39 71266.0 40 73427.0 41 75376.5 42 76461.5 43 76948.5 44 78271.0 45 81879.5 46 88872.5 47 107309.5 48 128751.5 49 222902.5 50 559775.5 51 559405.5 52 202383.0 53 78174.0 54 54082.0 55 45303.5 56 39167.5 57 33347.0 58 29917.5 59 25646.0 60 20509.0 61 16869.5 62 13443.0 63 10453.5 64 8049.5 65 6319.0 66 4971.0 67 4010.0 68 3402.0 69 2648.5 70 2101.0 71 1960.0 72 1818.0 73 1367.0 74 906.5 75 662.5 76 503.0 77 356.5 78 248.0 79 164.5 80 83.5 81 45.0 82 25.5 83 15.5 84 12.5 85 9.0 86 4.5 87 3.0 88 2.0 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.055662273381357714 2 0.0 3 2.7258703908598292E-5 4 4.088805586289744E-4 5 0.009622322479735197 6 6.542088938063591E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.3629351954299148E-4 16-17 3.1347509494888037E-4 18-19 0.0 20-21 0.0 22-23 4.088805586289744E-5 24-25 0.0038843653069752567 26-27 0.007278073943595744 28-29 0.0011721242680697267 30-31 0.00576521587666854 32-33 0.0025623181674082397 34-35 0.026427313439386046 36-37 0.025936656769031275 38-39 0.023387967953577338 40-41 0.0022624724244136585 42-43 0.005983285507937326 44-45 0.0071962978318699505 46-47 0.0010494601004810344 48-49 0.008763673306614352 50-51 0.010112979150089968 52-53 0.00175818640210459 54-55 0.011366879529885488 56-57 0.006828305329103873 58-59 0.011966571015874653 60-61 0.0077278425580876164 62-63 9.540546368009404E-4 64-65 0.013724757417979242 66-67 0.005288188558268069 68-69 0.0021943256646421625 70-71 0.008845449418340147 72-73 0.0051791537426336765 74-75 0.008845449418340147 76-77 0.0075915490385446245 78-79 0.015333020948586542 80-81 0.008763673306614352 82-83 0.011775760088514465 84-85 0.0067056411615151815 86-87 0.013438541026938958 88-89 0.02013055283649984 90-91 0.018317849026578053 92-93 0.017023060590919636 94-95 0.006160467083343214 96-97 0.010317419429404454 98-99 0.017500087909320106 100 0.0160008591943472 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3668553.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.53705570072153 #Duplication Level Percentage of deduplicated Percentage of total 1 89.5501261670525 39.882989571086576 2 6.419012373517857 5.7176782324597095 3 1.2025163802249235 1.6066961702132232 4 0.5924762712861761 1.0554859478251288 5 0.38460717560214724 0.8564635601345008 6 0.2769477088440186 0.7400661320983959 7 0.23112443018047207 0.7205521134521645 8 0.18227177142227932 0.6494278429202589 9 0.1404039250447467 0.5627859687286032 >10 0.9456641534289748 7.226572041485232 >50 0.03170480151117417 0.9874287125795334 >100 0.03289286666807746 3.043917040320689 >500 0.004693675400389886 1.3944508878797917 >1k 0.0019145254922621191 1.9362835020434919 >5k 0.0011734188500961377 4.0204645606312175 >10k+ 0.0024703554738866053 29.59873771614154 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG 318171 8.672929081302629 No Hit AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 174039 4.744077569548539 No Hit GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT 27907 0.7607086499772526 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 23032 0.627822468422836 No Hit AGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTC 20352 0.5547691419477925 No Hit TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG 20329 0.5541421917578947 No Hit CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA 19272 0.5253297417265064 No Hit CCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATT 19010 0.5181879613024536 No Hit CCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGAT 18060 0.49229219258928525 No Hit CTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCG 17879 0.4873583671818289 No Hit TCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGA 17541 0.4781449252607227 No Hit TCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGT 17395 0.47416515449006735 No Hit TACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACT 16969 0.46255294662500446 No Hit TCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCA 16649 0.45383016137425297 No Hit ACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTC 16469 0.4489235946707053 No Hit ACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGA 16289 0.4440170279671576 No Hit CGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAG 16056 0.43766574995645424 No Hit CCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTC 16036 0.43712057587828224 No Hit ATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTA 15785 0.43027864119722403 No Hit TGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCA 15573 0.42449979596860127 No Hit TCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACT 15533 0.42340944781225737 No Hit ACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAG 14951 0.40754488213745305 No Hit GAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGT 14214 0.38745521735681615 No Hit CTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGC 13991 0.38137652638519876 No Hit CAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTT 13918 0.37938664099987107 No Hit GTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCAC 13849 0.3775057904301778 No Hit AACGAGTGGAATCACTGCTGAGTACATGGTAACGAGTGGAATCACTGCTG 13819 0.37668802931291984 No Hit TGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATG 13242 0.36095975715765866 No Hit GATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGT 13223 0.3604418417833953 No Hit ATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCC 13079 0.3565165884205571 No Hit CGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAG 12323 0.3359090082656568 No Hit GTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCAT 12286 0.33490043622103866 No Hit CTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACG 11815 0.32206158668008883 No Hit TGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTG 11574 0.3154922390381167 No Hit TTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTAC 11447 0.3120303836417247 No Hit CTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGT 11392 0.31053115492675176 No Hit TGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGA 11025 0.3005272105922962 No Hit ATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGG 11010 0.3001183300336672 No Hit ATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCAC 10478 0.2856166995542929 No Hit CAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCC 10117 0.27577630744328896 No Hit ACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATC 9951 0.27125136259446164 No Hit TGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACA 9469 0.2581126673105173 No Hit ACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAAC 9298 0.25345142894214695 No Hit GCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCC 9295 0.2533696528304211 No Hit GGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACAT 8572 0.23366160990450457 No Hit GAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATG 8339 0.22731033189380118 No Hit TACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAAT 8301 0.22627450114527445 No Hit AGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGA 8144 0.2219948846316245 No Hit GGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGC 8068 0.21992322313457105 No Hit TCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGA 7718 0.21038267676656164 No Hit GGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTG 7435 0.2026684635604283 No Hit TGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACT 7168 0.19539038961683255 No Hit CACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAA 7085 0.19312791719241892 No Hit GAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGG 6623 0.1805343959866465 No Hit AACGAGTGGAATCACTGCTGAGTACATGGAAACGAGTGGAATCACTGCTG 6418 0.17494636168538386 No Hit GTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTAC 6394 0.1742921527915775 No Hit AGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCA 6282 0.1712391779538145 No Hit GAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCT 6249 0.17033964072483074 No Hit TACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG 5360 0.14610665295008685 No Hit GTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAA 4932 0.1344399276772068 No Hit GCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAG 4854 0.13231374877233612 No Hit CATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTC 3914 0.10669056709825372 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGTAACGAGTGGAATCACTGCT 3730 0.10167496557907164 No Hit CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 3727 0.10159318946734584 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.001935367977510479 0.0 0.0 0.0 0.0 2 0.001935367977510479 0.0 0.0 0.0 0.0 3 0.001935367977510479 0.0 0.0 0.0 0.0 4 0.001935367977510479 0.0 0.0 0.0 0.0 5 0.001935367977510479 0.0 0.0 0.0 0.0 6 0.001935367977510479 0.0 0.0 2.7258703908598296E-5 0.0 7 0.001935367977510479 0.0 0.0 2.7258703908598296E-5 0.0 8 0.0019626266814190773 0.0 0.0 2.7258703908598296E-5 0.0 9 0.0019898853853276754 0.0 0.0 2.7258703908598296E-5 2.7258703908598296E-5 10-11 0.0020716614970534702 0.0 0.0 2.7258703908598296E-5 2.7258703908598296E-5 12-13 0.002330619184185154 0.0 0.0 2.7258703908598296E-5 2.7258703908598296E-5 14-15 0.0028894226143114194 0.0 0.0 2.7258703908598296E-5 2.7258703908598296E-5 16-17 0.003625407619843573 0.0 0.0 5.451740781719659E-5 2.7258703908598296E-5 18-19 0.0043205045695128294 0.0 0.0 5.451740781719659E-5 2.7258703908598296E-5 20-21 0.005465370133673958 0.0 0.0 6.814675977149574E-5 2.7258703908598296E-5 22-23 0.006964598848646864 0.0 0.0 8.177611172579489E-5 2.7258703908598296E-5 24-25 0.008940854882020241 0.0 0.0 8.177611172579489E-5 2.7258703908598296E-5 26-27 0.011244215362296795 0.0 0.0 8.177611172579489E-5 2.7258703908598296E-5 28-29 0.013452170378893258 0.0 0.0 8.177611172579489E-5 2.7258703908598296E-5 30-31 0.016137152713890192 1.3629351954299148E-5 0.0 9.540546368009403E-5 2.7258703908598296E-5 32-33 0.021166383585026576 2.7258703908598296E-5 0.0 1.0903481563439318E-4 2.7258703908598296E-5 34-35 0.027640325763318672 2.7258703908598296E-5 0.0 1.0903481563439318E-4 2.7258703908598296E-5 36-37 0.03306480784112973 2.7258703908598296E-5 0.0 1.0903481563439318E-4 2.7258703908598296E-5 38-39 0.03956600872333042 2.7258703908598296E-5 0.0 1.2266416758869233E-4 2.7258703908598296E-5 40-41 0.04726659257750944 2.7258703908598296E-5 0.0 1.3629351954299148E-4 2.7258703908598296E-5 42-43 0.05507621124732286 2.7258703908598296E-5 0.0 1.6355222345158977E-4 2.7258703908598296E-5 44-45 0.06284494186127337 2.7258703908598296E-5 0.0 1.6355222345158977E-4 2.7258703908598296E-5 46-47 0.07004123969314333 2.7258703908598296E-5 0.0 1.6355222345158977E-4 2.7258703908598296E-5 48-49 0.07772819419536803 2.7258703908598296E-5 0.0 1.6355222345158977E-4 2.7258703908598296E-5 50-51 0.08966750650733409 2.7258703908598296E-5 0.0 1.6355222345158977E-4 2.7258703908598296E-5 52-53 0.10155230141148294 2.7258703908598296E-5 0.0 1.6355222345158977E-4 2.7258703908598296E-5 54-55 0.11597215577913145 2.7258703908598296E-5 0.0 1.9081092736018807E-4 2.7258703908598296E-5 56-57 0.13069185588977453 2.7258703908598296E-5 0.0 1.9081092736018807E-4 2.7258703908598296E-5 58-59 0.14276746172128357 2.7258703908598296E-5 0.0 1.9081092736018807E-4 2.7258703908598296E-5 60-61 0.1585911393402249 2.7258703908598296E-5 0.0 2.316989832230855E-4 2.7258703908598296E-5 62-63 0.179280495606851 2.7258703908598296E-5 0.0 2.7258703908598296E-4 2.7258703908598296E-5 64-65 0.20566692099037415 2.7258703908598296E-5 0.0 2.7258703908598296E-4 2.7258703908598296E-5 66-67 0.22949102820648903 2.7258703908598296E-5 0.0 2.9984574299458125E-4 2.7258703908598296E-5 68-69 0.2552641327520687 2.7258703908598296E-5 0.0 2.9984574299458125E-4 2.7258703908598296E-5 70-71 0.2863526845598251 2.7258703908598296E-5 0.0 2.9984574299458125E-4 2.7258703908598296E-5 72-73 0.317604788591033 2.7258703908598296E-5 0.0 3.1347509494888037E-4 2.7258703908598296E-5 74-75 0.3508467780075687 2.7258703908598296E-5 0.0 3.2710444690317955E-4 2.7258703908598296E-5 76-77 0.38482475242963643 2.7258703908598296E-5 0.0 3.407337988574787E-4 2.7258703908598296E-5 78-79 0.42032921427058567 2.7258703908598296E-5 0.0 3.5436315081177784E-4 2.7258703908598296E-5 80-81 0.46927221713847395 2.7258703908598296E-5 0.0 3.6799250276607696E-4 2.7258703908598296E-5 82-83 0.5263655724750331 2.7258703908598296E-5 0.0 3.8162185472037614E-4 2.7258703908598296E-5 84-85 0.5929313274198301 2.7258703908598296E-5 0.0 3.8162185472037614E-4 2.7258703908598296E-5 86-87 0.6675792880735265 2.7258703908598296E-5 0.0 4.3613926253757273E-4 2.7258703908598296E-5 88 0.7302606777113483 2.7258703908598296E-5 0.0 4.3613926253757273E-4 2.7258703908598296E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGAGT 2145 0.0 72.326965 1 ATCGAGT 895 0.0 57.780766 1 TCGAGTG 1120 0.0 53.706635 2 CGTGTGG 1665 0.0 53.061512 3 AACGTGT 1520 0.0 49.79617 1 ACGTGTG 1720 0.0 48.35939 2 AGTACTT 28720 0.0 36.85795 20-21 GAGTACT 28220 0.0 36.769936 20-21 GTACTTT 27050 0.0 36.72801 22-23 GTGTGGA 2580 0.0 36.611065 4 TACTTTT 28925 0.0 35.21644 22-23 CGATTGG 660 0.0 34.176952 3 ACTTTTT 29355 0.0 33.66111 24-25 ACGATTG 690 0.0 32.009933 2 ACGCACG 270 0.0 31.333998 94 ATGAGTG 2005 0.0 29.531954 2 GTGGAAT 279495 0.0 29.482403 6 TGGAATC 281245 0.0 29.391718 7 AGTGGAA 279795 0.0 29.126968 5 GGAATCA 285055 0.0 29.01536 8 >>END_MODULE