##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132710_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3596599
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.914163630696667	33.0	31.0	34.0	25.0	34.0
2	31.653909151395528	34.0	31.0	34.0	28.0	34.0
3	31.857361913296423	34.0	31.0	34.0	28.0	34.0
4	35.79830806826116	37.0	35.0	37.0	35.0	37.0
5	35.451168729124376	37.0	35.0	37.0	33.0	37.0
6	35.80173019010459	37.0	35.0	37.0	35.0	37.0
7	35.98273118576744	37.0	35.0	37.0	35.0	37.0
8	36.122277462680714	37.0	37.0	37.0	35.0	37.0
9	38.04346634139641	39.0	39.0	39.0	37.0	39.0
10-11	38.10856992953621	39.0	39.0	39.0	37.0	39.0
12-13	38.08607381584658	39.0	39.0	39.0	37.0	39.0
14-15	39.62076617382144	41.0	40.0	41.0	38.0	41.0
16-17	39.54645611034202	41.0	40.0	41.0	37.0	41.0
18-19	39.47679210276152	41.0	40.0	41.0	37.0	41.0
20-21	39.356668619437414	41.0	39.5	41.0	36.0	41.0
22-23	39.252461561603056	41.0	39.5	41.0	36.0	41.0
24-25	39.11933412649006	41.0	39.0	41.0	36.0	41.0
26-27	39.082273419972594	41.0	39.0	41.0	35.5	41.0
28-29	38.967490537588425	41.0	39.0	41.0	35.0	41.0
30-31	38.8409154036911	41.0	39.0	41.0	35.0	41.0
32-33	38.709195687370205	41.0	39.0	41.0	35.0	41.0
34-35	38.596278873457955	41.0	39.0	41.0	35.0	41.0
36-37	38.38543968899508	40.5	38.5	41.0	35.0	41.0
38-39	38.03270492484705	40.0	37.0	41.0	34.5	41.0
40-41	37.88528023279771	40.0	37.0	41.0	34.0	41.0
42-43	37.792102900545764	40.0	37.0	41.0	34.0	41.0
44-45	37.62857341060263	40.0	37.0	41.0	33.5	41.0
46-47	37.52337555562908	40.0	36.0	41.0	33.0	41.0
48-49	37.37046345728284	40.0	36.0	41.0	33.0	41.0
50-51	37.130310607326535	40.0	35.5	41.0	33.0	41.0
52-53	36.67961927365269	40.0	35.0	41.0	32.0	41.0
54-55	35.8765732571243	39.0	35.0	41.0	29.0	41.0
56-57	35.3927043298405	39.0	35.0	41.0	26.0	41.0
58-59	35.1885468744222	39.0	35.0	41.0	25.0	41.0
60-61	34.941463727260114	39.0	35.0	41.0	24.0	41.0
62-63	34.71540294038896	39.0	35.0	41.0	23.0	41.0
64-65	34.38257489923119	38.0	35.0	40.5	22.5	41.0
66-67	33.947913153509745	37.5	34.5	40.0	21.0	41.0
68-69	33.70917956102418	37.0	34.0	40.0	22.0	41.0
70-71	33.41894022658629	36.5	34.0	39.0	20.5	41.0
72-73	33.07991841737152	36.0	34.0	39.0	20.0	41.0
74-75	32.7181323244543	35.5	34.0	39.0	18.5	40.0
76-77	32.334866355687694	35.0	34.0	37.5	16.0	40.0
78-79	31.974857497318993	35.0	33.0	37.0	12.5	39.0
80-81	31.62573489566115	35.0	33.0	36.5	9.0	39.0
82-83	31.20182358389134	35.0	32.5	36.0	7.0	38.5
84-85	30.854182798805205	35.0	32.0	36.0	3.5	37.0
86-87	30.786899929627964	35.0	32.5	35.0	2.0	37.0
88-89	30.874695649973766	35.0	33.0	35.0	2.0	37.0
90-91	30.71106370212526	35.0	33.0	35.0	2.0	36.0
92-93	30.580105538593543	35.0	33.0	35.0	2.0	36.0
94-95	30.368519537485273	35.0	33.0	35.0	2.0	36.0
96-97	30.097140381788464	35.0	32.5	35.0	2.0	36.0
98-99	30.0059999738642	35.0	33.0	35.0	2.0	35.0
100	29.97870404790748	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.8795309420429156
1101	2	1.3808678911369512
1101	3	1.2109271817296339
1101	4	0.6288898020734024
1101	5	0.9457759977275444
1101	6	0.5816259883606563
1101	7	0.3542301108568182
1101	8	0.2642671197128408
1101	9	0.24792617816876117
1101	10-11	0.15061635043922195
1101	12-13	0.2092367145769103
1101	14-15	0.27329874275861243
1101	16-17	0.2512215765573984
1101	18-19	0.2673253124198709
1101	20-21	0.35529418763713494
1101	22-23	0.343413585778066
1101	24-25	0.3168067459907107
1101	26-27	0.2978661060350234
1101	28-29	0.3999116545426915
1101	30-31	0.4428756371007836
1101	32-33	0.43157233650102
1101	34-35	0.4062947216024355
1101	36-37	0.46869192158079187
1101	38-39	0.5343251573676966
1101	40-41	0.5265197975861184
1101	42-43	0.5267584750992924
1101	44-45	0.5450149071952168
1101	46-47	0.5465678904170801
1101	48-49	0.4308026077102909
1101	50-51	0.4368765257124423
1101	52-53	0.5117136153212201
1101	54-55	0.5524206208370614
1101	56-57	0.5634218899416581
1101	58-59	0.5016090159878814
1101	60-61	0.47454843035460925
1101	62-63	0.47208233693822876
1101	64-65	0.4924057689608148
1101	66-67	0.48562694721479716
1101	68-69	0.6163802755354482
1101	70-71	0.5107203501894588
1101	72-73	0.526822719263798
1101	74-75	0.49440263620353875
1101	76-77	0.4848355562060789
1101	78-79	0.48103419417095594
1101	80-81	0.38298204240974343
1101	82-83	0.3535683238614986
1101	84-85	0.39269460811991763
1101	86-87	0.43683667554189753
1101	88-89	0.38119961085643794
1101	90-91	0.44662318654152955
1101	92-93	0.4753891246497304
1101	94-95	0.43189331553930543
1101	96-97	0.4801013728648016
1101	98-99	0.5630950459090656
1101	100	0.5308733391049323
1102	1	1.3482476548819555
1102	2	1.1638159758817181
1102	3	1.1005958364331896
1102	4	0.5633423990300699
1102	5	0.8910264938099957
1102	6	0.4906423901147434
1102	7	0.3090403238504891
1102	8	0.2515794216253511
1102	9	0.22100435238908744
1102	10-11	0.20374725912751046
1102	12-13	0.2102074546666728
1102	14-15	0.3007884281851858
1102	16-17	0.3144739083043646
1102	18-19	0.31960339924005865
1102	20-21	0.38994889740703087
1102	22-23	0.38229311244353426
1102	24-25	0.371784951563221
1102	26-27	0.35194107601984825
1102	28-29	0.4325320296665538
1102	30-31	0.4457730153747548
1102	32-33	0.41854016510647796
1102	34-35	0.4153488907148315
1102	36-37	0.37961114481862523
1102	38-39	0.3715517886665225
1102	40-41	0.3314856552727221
1102	42-43	0.3257950578782953
1102	44-45	0.4246030872100661
1102	46-47	0.3063201852839441
1102	48-49	0.377702180309889
1102	50-51	0.36797225584620463
1102	52-53	0.2886857321593723
1102	54-55	0.3037386349960798
1102	56-57	0.27130476394726344
1102	58-59	0.26740870841921094
1102	60-61	0.2071517310508142
1102	62-63	0.2756600387497059
1102	64-65	0.25509301729086786
1102	66-67	0.27857700353260384
1102	68-69	0.2756805968912204
1102	70-71	0.26010479581108115
1102	72-73	0.3802480800551251
1102	74-75	0.3156400227563054
1102	76-77	0.3132133122174068
1102	78-79	0.4126648494413665
1102	80-81	0.3391098402485184
1102	82-83	0.362011579274494
1102	84-85	0.3474917372609774
1102	86-87	0.27974826124432184
1102	88-89	0.2782039115187942
1102	90-91	0.26849998506017414
1102	92-93	0.296998440156532
1102	94-95	0.3065892999577464
1102	96-97	0.2361478913579269
1102	98-99	0.2655928106146206
1102	100	0.24965923281465763
1103	1	1.8067254873549707
1103	2	1.2797235400466747
1103	3	1.1741156743154413
1103	4	0.5905199387018456
1103	5	0.8804748786162406
1103	6	0.508556480476237
1103	7	0.3112029841132937
1103	8	0.23400887407905913
1103	9	0.2675905452549472
1103	10-11	0.1888140768487645
1103	12-13	0.20194319955461992
1103	14-15	0.2966326052700907
1103	16-17	0.30251995867894266
1103	18-19	0.2813420960751003
1103	20-21	0.3602778400514879
1103	22-23	0.40193512902230566
1103	24-25	0.3703103742102414
1103	26-27	0.3549742814354815
1103	28-29	0.3716059958867106
1103	30-31	0.3564162207105994
1103	32-33	0.314031105820213
1103	34-35	0.3231249177131801
1103	36-37	0.38855422808747164
1103	38-39	0.38033339245140496
1103	40-41	0.34994828920735443
1103	42-43	0.37223808131037117
1103	44-45	0.3733286040002568
1103	46-47	0.33489798504619017
1103	48-49	0.4004673967993
1103	50-51	0.3685060795750701
1103	52-53	0.4151865579067291
1103	54-55	0.35505237112392507
1103	56-57	0.3168388669143667
1103	58-59	0.29754880623237767
1103	60-61	0.28156776411262285
1103	62-63	0.2945765544947534
1103	64-65	0.2639684052325677
1103	66-67	0.3390580512344741
1103	68-69	0.2524857923180974
1103	70-71	0.2904482588439308
1103	72-73	0.2784824965668804
1103	74-75	0.32070209342752065
1103	76-77	0.3686635639307383
1103	78-79	0.37506733545951576
1103	80-81	0.376063989329829
1103	82-83	0.380510873200663
1103	84-85	0.32998062756821867
1103	86-87	0.32167859529303655
1103	88-89	0.34430482821803565
1103	90-91	0.37564090463158095
1103	92-93	0.40296280935735496
1103	94-95	0.3512798640987711
1103	96-97	0.3215026834850043
1103	98-99	0.3202610356965998
1103	100	0.35345049026433273
1104	1	1.8710822310806812
1104	2	1.361452651114142
1104	3	1.2406756675376336
1104	4	0.613141794819775
1104	5	0.8972568835378567
1104	6	0.5392502429680022
1104	7	0.25438331614883225
1104	8	0.19443903378114413
1104	9	0.18051320508219248
1104	10-11	0.1750021378952411
1104	12-13	0.18358959056477886
1104	14-15	0.29093643588652895
1104	16-17	0.2846094873517657
1104	18-19	0.2858863462994208
1104	20-21	0.2899215045346253
1104	22-23	0.3232540287069128
1104	24-25	0.3477681958383769
1104	26-27	0.31564524584813825
1104	28-29	0.332685323189331
1104	30-31	0.37247373194597344
1104	32-33	0.37410854997069976
1104	34-35	0.37330541534286965
1104	36-37	0.3717459976728321
1104	38-39	0.33607962518436096
1104	40-41	0.38448526099087843
1104	42-43	0.3500540869482762
1104	44-45	0.3342060989842892
1104	46-47	0.35134063747945987
1104	48-49	0.30735730749399437
1104	50-51	0.3724901209197
1104	52-53	0.46365292496350463
1104	54-55	0.356654244263396
1104	56-57	0.27944928548248527
1104	58-59	0.284750919675929
1104	60-61	0.2978199447565899
1104	62-63	0.3361605588695227
1104	64-65	0.36182030244095387
1104	66-67	0.3879621260060162
1104	68-69	0.3791992489613065
1104	70-71	0.3713200928184577
1104	72-73	0.3469697361893225
1104	74-75	0.3677446588275828
1104	76-77	0.4123269302673691
1104	78-79	0.3071489310169646
1104	80-81	0.34153194686070165
1104	82-83	0.381353015757977
1104	84-85	0.3413436985310909
1104	86-87	0.2974641470104018
1104	88-89	0.27805491967992424
1104	90-91	0.3495515454039477
1104	92-93	0.35504609936663556
1104	94-95	0.35877546430056384
1104	96-97	0.36762650940318764
1104	98-99	0.380427477078193
1104	100	0.43361616144055404
1105	1	1.6241921758798092
1105	2	1.2859350483750234
1105	3	1.1947502984247542
1105	4	0.5675886051372174
1105	5	0.9413356585717878
1105	6	0.5847295209608774
1105	7	0.3106923415973668
1105	8	0.23247824277180484
1105	9	0.24234351612385296
1105	10-11	0.20201697280258912
1105	12-13	0.2095650938195064
1105	14-15	0.2494926837307716
1105	16-17	0.2610972203327151
1105	18-19	0.2855961287174793
1105	20-21	0.32001146262437175
1105	22-23	0.2975938670177882
1105	24-25	0.3478402353981238
1105	26-27	0.26864744582545796
1105	28-29	0.32142532283288716
1105	30-31	0.281086308435583
1105	32-33	0.3213142238124078
1105	34-35	0.31770907231655343
1105	36-37	0.3093834026491322
1105	38-39	0.3550207125503704
1105	40-41	0.3253187661857382
1105	42-43	0.3514990717143789
1105	44-45	0.40328754367551056
1105	46-47	0.4230785971004636
1105	48-49	0.39590699389725614
1105	50-51	0.36745967035194127
1105	52-53	0.33265576266317254
1105	54-55	0.2665600132242929
1105	56-57	0.38982224072344707
1105	58-59	0.46088490996211817
1105	60-61	0.4843056528418046
1105	62-63	0.4866560292885964
1105	64-65	0.4372122852616869
1105	66-67	0.4483541545067169
1105	68-69	0.5096653254427537
1105	70-71	0.4476936333621282
1105	72-73	0.48021869637091896
1105	74-75	0.46381993312831327
1105	76-77	0.500601862881922
1105	78-79	0.5058996362483121
1105	80-81	0.48386148606063273
1105	82-83	0.5055580906678863
1105	84-85	0.4897799009110315
1105	86-87	0.442843610523866
1105	88-89	0.36831955198536903
1105	90-91	0.36264971525465484
1105	92-93	0.4550466684941341
1105	94-95	0.4503869068785207
1105	96-97	0.4688304836504642
1105	98-99	0.390547237978744
1105	100	0.47470769107105326
1106	1	1.5811068842575473
1106	2	1.2790828986629492
1106	3	1.1056438786822689
1106	4	0.6012196890892554
1106	5	0.9457768727331839
1106	6	0.5879069309852056
1106	7	0.32069624271750996
1106	8	0.18306674893871389
1106	9	0.18937810545426714
1106	10-11	0.15171904775822043
1106	12-13	0.11939427455902063
1106	14-15	0.23049213555302117
1106	16-17	0.21917013916090866
1106	18-19	0.2155582290285949
1106	20-21	0.30188148495206235
1106	22-23	0.2691597896326954
1106	24-25	0.26396102793540877
1106	26-27	0.25218706456946194
1106	28-29	0.37878871415853865
1106	30-31	0.3616937175324466
1106	32-33	0.38925255580026885
1106	34-35	0.34038381886102087
1106	36-37	0.32688303003983066
1106	38-39	0.384058651575252
1106	40-41	0.3523715727728245
1106	42-43	0.3582961510348639
1106	44-45	0.36944559714287095
1106	46-47	0.3469272704489583
1106	48-49	0.3304609940971801
1106	50-51	0.33087006855128465
1106	52-53	0.37133240960530145
1106	54-55	0.36854509152998816
1106	56-57	0.40592894087021847
1106	58-59	0.38849473587688976
1106	60-61	0.3301539732490042
1106	62-63	0.35631224352756874
1106	64-65	0.2945417718475767
1106	66-67	0.2290767025841447
1106	68-69	0.354731563286947
1106	70-71	0.39534159353822673
1106	72-73	0.3975495579121855
1106	74-75	0.34850448191378547
1106	76-77	0.35848438106399527
1106	78-79	0.40470878414046396
1106	80-81	0.44378703998540914
1106	82-83	0.4595625309437601
1106	84-85	0.40843757635587963
1106	86-87	0.47266052853790796
1106	88-89	0.4216442735027712
1106	90-91	0.39369095705950485
1106	92-93	0.4354828757969855
1106	94-95	0.4009845974587023
1106	96-97	0.4164806451987033
1106	98-99	0.4321882490364892
1106	100	0.49152429764507133
1107	1	1.6046347430692727
1107	2	1.279005206643916
1107	3	1.187519952369005
1107	4	0.6503613997091122
1107	5	0.998483741876818
1107	6	0.6622374501828645
1107	7	0.4092089496997957
1107	8	0.3464598535834469
1107	9	0.33647926913739923
1107	10-11	0.24898587270038064
1107	12-13	0.18189392098490487
1107	14-15	0.30241571074361673
1107	16-17	0.30603038215589606
1107	18-19	0.2893990245043554
1107	20-21	0.33536146511419673
1107	22-23	0.31795075472975043
1107	24-25	0.2672557729754743
1107	26-27	0.326315433659353
1107	28-29	0.36805156398476413
1107	30-31	0.39585689717260664
1107	32-33	0.40159879820313904
1107	34-35	0.33771985004343463
1107	36-37	0.3292737567807862
1107	38-39	0.30592141201794476
1107	40-41	0.32591706314712354
1107	42-43	0.3682373351592929
1107	44-45	0.3981454438331369
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2215	4	-1.8009208145909597
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2216	2	-6.292039410729114
2216	3	-5.603447957418648
2216	4	-3.054746748204238
2216	5	-4.143483036463099
2216	6	-4.35598328507302
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2216	9	-1.1115069728054578
2216	10-11	-0.9025958593970813
2216	12-13	-0.7536866215877325
2216	14-15	-1.050294562224103
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2216	34-35	-1.3901081978832437
2216	36-37	-1.4014097327003014
2216	38-39	-1.41950913186038
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2216	54-55	-1.4612820427328899
2216	56-57	-1.4279993320390574
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2216	60-61	-1.512305047500881
2216	62-63	-1.6025511464059008
2216	64-65	-1.6413073894092136
2216	66-67	-1.6057265132753926
2216	68-69	-1.616818592126286
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2216	78-79	-1.6871651696901147
2216	80-81	-1.7120837453249464
2216	82-83	-1.6516685198128194
2216	84-85	-1.6274123728594212
2216	86-87	-1.6393423198548547
2216	88-89	-1.6034501965425996
2216	90-91	-1.6185066406867463
2216	92-93	-1.5747316930305715
2216	94-95	-1.6150738424885063
2216	96-97	-1.6111271343929268
2216	98-99	-1.706642562675107
2216	100	-1.697490479203534
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4229.0
3	3361.0
4	4117.0
5	5630.0
6	4014.0
7	3161.0
8	3031.0
9	3446.0
10	3821.0
11	7541.0
12	6105.0
13	8180.0
14	7712.0
15	8302.0
16	9831.0
17	12657.0
18	15507.0
19	23189.0
20	48129.0
21	71421.0
22	32799.0
23	19727.0
24	19977.0
25	20979.0
26	21976.0
27	23229.0
28	26135.0
29	30889.0
30	36805.0
31	45639.0
32	59524.0
33	81219.0
34	120014.0
35	249697.0
36	383756.0
37	742583.0
38	1009686.0
39	415316.0
40	3265.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.516163896527694	42.847811360699495	25.766757497736176	17.86926724503663
2	17.15804291776759	39.863493261272666	25.437892853776585	17.540570967183164
3	18.252410124120036	24.194468162839396	19.867352462701568	37.685769250339
4	34.08615015204718	25.2192290400244	22.549165443514273	18.145455364414147
5	17.228194163829	39.1548780372509	23.57692515172699	20.040002647193113
6	37.26354358138383	21.699035726310985	24.087852031074963	16.949568661230224
7	18.986937381676412	22.446511273567054	40.32857151992758	18.237979824828958
8	36.1701985681473	22.762281811233333	23.141250942904673	17.926268677714695
9	35.96475448055232	24.336296595756156	23.59854406899407	16.10040485469745
10-11	16.43754280085158	42.4883341178708	23.43808692600982	17.636036155267796
12-13	16.35845419519941	23.601268865392	32.51150600887116	27.528770930537434
14-15	16.94149157271896	32.85941723180799	22.82011058799258	27.378980607480468
16-17	26.74497603910594	23.017625447663683	32.36361836678887	17.87378014644151
18-19	17.397132318216784	23.671838822153767	32.28968982776365	26.6413390318658
20-21	27.03597203914031	32.94474863614209	23.249714521969228	16.769564802748373
22-23	26.22326709851921	23.576557624733148	32.608134131198426	17.59204114554921
24-25	16.86284868609927	32.81824633892195	23.20877755062461	27.110127424354168
26-27	17.35924568626031	28.782367906602524	36.413219760097	17.44516664704017
28-29	28.605153323195847	23.126824490004736	30.967373482331556	17.30064870446786
30-31	24.028991741332696	26.50332477988079	31.438254912164332	18.029428566622183
32-33	19.060974643368056	27.270356277431222	32.18857526564057	21.480093813560146
34-35	20.722292421997945	27.18757356897097	32.430975357862415	19.65915865116867
36-37	21.12515928650769	25.435253337160102	34.30669834876	19.132889027572208
38-39	23.308428129957218	25.52860751861633	32.584805248836965	18.57815910258949
40-41	18.584873566855382	29.895741852751236	32.49978347037145	19.019601110021934
42-43	18.6154772508967	25.6181990045864	34.54490323572678	21.221420508790125
44-45	18.67621966046498	27.812361361537967	31.94914190308283	21.56227707491423
46-47	21.149524711974454	25.45821613788302	33.97429374101223	19.41796540913029
48-49	19.16877360530163	25.875602450050433	33.87509636767719	21.080527576970752
50-51	21.3794823639069	27.920340534108885	31.84516965391286	18.855007448071355
52-53	20.996439203214546	25.96791654392092	33.49972536001056	19.535918892853978
54-55	19.338936254994483	28.444638660771215	30.341839582072172	21.874585502162134
56-57	19.734801299091302	29.08471343247061	31.161382620204876	20.019102648233215
58-59	24.24133849104422	27.262317204780977	28.725388044888877	19.770956259285924
60-61	21.731721911905698	29.560359900431777	28.588743852062436	20.11917433560009
62-63	19.6194187682284	29.3458330848314	28.43810244341003	22.596645703530175
64-65	21.41578780229125	29.36426168440524	28.70827653597574	20.511673977327774
66-67	21.846235124012903	27.23686186186186	30.867951284617952	20.048951729507287
68-69	23.72170333249039	27.40914849641229	29.299006023748824	19.570142147348495
70-71	19.13552346179904	31.41160082846178	29.348232150039617	20.104643559699564
72-73	19.065574659350162	27.37779217475083	31.374180264623163	22.18245290127585
74-75	19.30426450328332	29.39392519576824	28.84394028775008	22.45787001319836
76-77	21.692683999072933	27.146501177533448	30.678281233555094	20.48253358983852
78-79	19.972797881894365	27.649262307310803	30.255223553330207	22.122716257464628
80-81	22.029982308144163	29.617070381608812	28.508093415779467	19.84485389446756
82-83	21.64515953668452	27.778143131499117	30.320680616443337	20.25601671537303
84-85	19.747905935897336	29.86336725285261	28.049823209938058	22.338903601311994
86-87	19.865433904193676	30.029954248306034	29.811957284329832	20.292654563170455
88-89	24.02262865930686	27.911813025036274	27.979348602185972	20.086209713470893
90-91	21.698699034968826	30.15740503333476	27.87561326004339	20.268282671653022
92-93	19.83174395492779	30.031305552060168	27.488721867237487	22.648228625774557
94-95	21.59416314449899	30.313048771010138	27.379897917035382	20.71289016745549
96-97	21.96598825306586	28.238592481211374	29.323331268686964	20.472087997035807
98-99	23.892223669964153	28.568878133793135	27.416410052053358	20.122488144189347
100	19.718838222829316	32.26441654189868	27.589121047561633	20.42762418771037
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	64483.0
1	34982.0
2	4138.0
3	2339.0
4	1881.0
5	1786.0
6	1922.0
7	2372.5
8	2229.5
9	2026.0
10	2131.5
11	3027.5
12	27664.5
13	31210.5
14	10249.0
15	9740.5
16	10889.5
17	12544.5
18	13796.5
19	14838.5
20	16396.0
21	18296.5
22	20445.5
23	23319.5
24	26603.0
25	30121.0
26	34527.5
27	39667.5
28	44012.0
29	48118.0
30	52665.0
31	56921.0
32	61402.5
33	66356.5
34	71775.5
35	75634.5
36	79881.0
37	83773.5
38	85962.0
39	87978.5
40	88958.0
41	90202.5
42	90445.0
43	90988.5
44	93435.0
45	94420.5
46	96200.5
47	106029.5
48	113554.0
49	148546.5
50	292830.0
51	293318.0
52	138332.5
53	84236.5
54	73516.5
55	68270.5
56	62703.5
57	57136.0
58	53721.5
59	47173.5
60	37579.5
61	30576.5
62	24403.0
63	18178.5
64	13731.5
65	10666.0
66	7898.0
67	5830.5
68	4380.5
69	3303.0
70	2459.5
71	1842.5
72	1501.5
73	1210.0
74	914.0
75	701.0
76	533.5
77	376.5
78	235.0
79	144.5
80	103.5
81	68.0
82	34.0
83	15.5
84	8.5
85	6.0
86	5.0
87	4.0
88	2.0
89	1.0
90	0.5
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05599734638195695
2	0.0
3	0.0
4	5.004728077831307E-4
5	0.00936996312349528
6	1.6682426926104354E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.6682426926104354E-4
16-17	2.6413842632998566E-4
18-19	1.3902022438420298E-5
20-21	0.0
22-23	2.7804044876840596E-5
24-25	0.00421231279884135
26-27	0.00752099413918538
28-29	0.0012789860643346673
30-31	0.005435690773422336
32-33	0.0028916206671914216
34-35	0.02525997477060968
36-37	0.0243841473569892
38-39	0.023105161292654535
40-41	0.0025996781959845955
42-43	0.005685927177313901
44-45	0.006659068748003322
46-47	0.0011955739297041456
48-49	0.009634101549825266
50-51	0.010176280424923658
52-53	0.002015793253570943
54-55	0.010565537053199426
56-57	0.006645166725564902
58-59	0.011955739297041454
60-61	0.007534896161623801
62-63	0.0013206921316499282
64-65	0.013373745585760325
66-67	0.005533004930491278
68-69	0.00234944179209303
70-71	0.008216095261106395
72-73	0.0045598633598018575
74-75	0.00885558829327373
76-77	0.008633155934259003
78-79	0.015445146929084948
80-81	0.008660959979135845
82-83	0.01080187143465257
84-85	0.006756382905072264
86-87	0.012456212104824586
88-89	0.019921598154256284
90-91	0.018156041304576907
92-93	0.01644609254465121
94-95	0.005852751446574945
96-97	0.009495081325441062
98-99	0.016056835916375443
100	0.01643219052221279
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3596599.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.14235824989326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.19470191516778	45.04357355584096
2	11.376349032309314	12.31884729766235
3	2.6316717424731513	4.274547428313067
4	0.9061759981056097	1.9625002210755405
5	0.4795438531931549	1.298181754805901
6	0.3076342721331756	0.9993626983067732
7	0.19168110260831703	0.7264646849007836
8	0.13836603973623726	0.599317095441466
9	0.10394111240732244	0.506485525036471
>10	0.6187450883338582	7.073602429769782
>50	0.028147257113384526	1.0161126640399718
>100	0.01740346598076728	1.9299632419671344
>500	0.001293376247528743	0.4612501559230312
>1k	0.002276342194994725	3.0455339127830023
>5k	0.0018107267460185312	6.77141670724861
>10k+	2.586752494312187E-4	11.972840626885132
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	218503	6.0752672177243	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	122054	3.393594893397902	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	62483	1.7372801360396306	No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT	13712	0.3812490633512382	No Hit
TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG	10688	0.29716963164367227	No Hit
AGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTC	9672	0.26892072204880224	No Hit
CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA	9361	0.2602736640921048	No Hit
CTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCG	8997	0.25015299175693484	No Hit
CCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATT	8957	0.2490408299618612	No Hit
TCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGA	8588	0.238781137402307	No Hit
CCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGAT	8358	0.23238620708063368	No Hit
TACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACT	8159	0.2268532021501424	No Hit
TCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGT	8112	0.22554641204093087	No Hit
TCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCA	8078	0.2246010745151183	No Hit
CCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTC	8057	0.22401718957270467	No Hit
CGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAG	7956	0.22120898104014378	No Hit
ATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTA	7624	0.2119780381410327	No Hit
TCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACT	7616	0.21175560578201796	No Hit
ACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGA	7405	0.20588895231300458	No Hit
TGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCA	7334	0.2039148651267489	No Hit
ACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTC	7216	0.20063398783128172	No Hit
CTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGC	7097	0.19732530649093769	No Hit
ACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAG	6591	0.18325645978325636	No Hit
TGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATG	6502	0.18078189978921755	No Hit
CAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTT	6306	0.17533230699335678	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGTAACGAGTGGAATCACTGCTG	6263	0.17413673306365263	No Hit
GTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCAC	6242	0.173552848121239	No Hit
GATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGT	6073	0.16885396453705293	No Hit
GTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCAT	5931	0.16490579016454157	No Hit
GAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGT	5822	0.16187514927296592	No Hit
CTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACG	5566	0.15475731378449475	No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5525	0.1536173479445443	No Hit
TTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTAC	5442	0.1513096122197665	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5432	0.1510315717709981	No Hit
ATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCC	5409	0.15039207873883076	No Hit
CGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAG	5381	0.14961356548227922	No Hit
CTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGT	5381	0.14961356548227922	No Hit
ATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGG	5226	0.14530393852636894	No Hit
TGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTG	5049	0.14038262258316817	No Hit
TGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGA	5017	0.13949289314710925	No Hit
ATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCAC	4562	0.12684205272814678	No Hit
ACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAAC	4335	0.12053053454110396	No Hit
ACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATC	4326	0.1202802981372124	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4318	0.12005786577819767	No Hit
GCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCC	4179	0.11619310354031684	No Hit
TGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACA	4091	0.11374634759115487	No Hit
CAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCC	4067	0.11307905051411068	No Hit
TACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAAT	3683	0.1024022972814039	No Hit
GAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATG	3610	0.10037260200539454	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0031696611159598275	0.0	0.0	2.7804044876840593E-5	0.0
2	0.0031696611159598275	0.0	0.0	2.7804044876840593E-5	0.0
3	0.0031974651608366682	0.0	0.0	2.7804044876840593E-5	0.0
4	0.003225269205713509	0.0	0.0	8.341213463052179E-5	0.0
5	0.003225269205713509	2.7804044876840593E-5	0.0	1.1121617950736237E-4	2.7804044876840593E-5
6	0.003225269205713509	5.5608089753681186E-5	0.0	1.3902022438420296E-4	5.5608089753681186E-5
7	0.003225269205713509	5.5608089753681186E-5	0.0	1.3902022438420296E-4	5.5608089753681186E-5
8	0.003225269205713509	5.5608089753681186E-5	0.0	1.3902022438420296E-4	5.5608089753681186E-5
9	0.003225269205713509	5.5608089753681186E-5	0.0	1.6682426926104357E-4	5.5608089753681186E-5
10-11	0.003225269205713509	6.951011219210148E-5	0.0	1.6682426926104357E-4	8.341213463052179E-5
12-13	0.0033503874076592915	8.341213463052179E-5	0.0	1.6682426926104357E-4	1.1121617950736237E-4
14-15	0.003517211676920335	8.341213463052179E-5	0.0	1.6682426926104357E-4	1.2511820194578265E-4
16-17	0.003795252125688741	9.731415706894208E-5	0.0	2.2243235901472474E-4	1.6682426926104357E-4
18-19	0.003948174372511364	1.2511820194578265E-4	0.0	2.2243235901472474E-4	1.8072629169946388E-4
20-21	0.004365235045663973	2.0853033657630444E-4	0.0	2.5023640389156536E-4	2.5023640389156536E-4
22-23	0.005004728077831307	2.6413842632998566E-4	0.0	2.6413842632998566E-4	2.6413842632998566E-4
24-25	0.005241062459284452	3.1974651608366683E-4	0.0	3.0584449364524653E-4	3.0584449364524653E-4
26-27	0.005783241334382844	3.3364853852208714E-4	0.0	3.0584449364524653E-4	3.3364853852208714E-4
28-29	0.006367126276796496	3.475505609605074E-4	0.0	3.0584449364524653E-4	3.3364853852208714E-4
30-31	0.006964913241648569	3.614525833989277E-4	0.0	3.614525833989277E-4	3.3364853852208714E-4
32-33	0.007826838632830627	3.614525833989277E-4	0.0	3.75354605837348E-4	3.3364853852208714E-4
34-35	0.009091922674726874	3.892566282757683E-4	0.0	4.865707853447104E-4	3.475505609605074E-4
36-37	0.010078966267854716	4.1706067315260887E-4	0.0	5.004728077831307E-4	3.614525833989277E-4
38-39	0.0112328341302436	4.587667404678698E-4	0.0	5.004728077831307E-4	3.614525833989277E-4
40-41	0.012859370755538774	5.004728077831307E-4	0.0	5.004728077831307E-4	3.892566282757683E-4
42-43	0.014319083111572905	5.004728077831307E-4	0.0	5.14374830221551E-4	3.892566282757683E-4
44-45	0.016056835916375443	5.004728077831307E-4	0.0	5.282768526599712E-4	3.892566282757683E-4
46-47	0.017447038160217473	5.421788750983915E-4	0.0	5.421788750983915E-4	3.892566282757683E-4
48-49	0.019073574785512647	5.560808975368118E-4	0.0	5.560808975368118E-4	4.031586507141886E-4
50-51	0.021172780173714115	5.560808975368118E-4	0.0	5.560808975368118E-4	4.1706067315260887E-4
52-53	0.023508319943368723	5.838849424136524E-4	0.0	5.699829199752322E-4	4.1706067315260887E-4
54-55	0.02620531229642226	6.533950546057539E-4	0.0	5.838849424136524E-4	4.309626955910292E-4
56-57	0.02895791273922948	6.951011219210148E-4	0.0	5.977869648520727E-4	5.004728077831307E-4
58-59	0.031988553630805105	7.229051667978554E-4	0.0	6.394930321673337E-4	5.14374830221551E-4
60-61	0.03590892395843963	7.229051667978554E-4	0.0	6.672970770441743E-4	5.421788750983915E-4
62-63	0.040885847991394095	7.229051667978554E-4	0.0	6.672970770441743E-4	5.699829199752322E-4
64-65	0.04751711269452057	7.646112341131164E-4	0.0	6.951011219210148E-4	5.838849424136524E-4
66-67	0.054120573352770214	7.924152789899569E-4	0.0	7.229051667978554E-4	5.838849424136524E-4
68-69	0.06210033423242346	8.202193238667975E-4	0.0	7.229051667978554E-4	5.838849424136524E-4
70-71	0.07073349016668246	8.341213463052177E-4	0.0	7.368071892362757E-4	5.838849424136524E-4
72-73	0.0807568483447835	8.619253911820584E-4	0.0	7.50709211674696E-4	5.838849424136524E-4
74-75	0.09204529056478078	8.619253911820584E-4	0.0	7.646112341131164E-4	5.838849424136524E-4
76-77	0.1044597966022901	8.758274136204786E-4	0.0	7.785132565515366E-4	6.255910097289134E-4
78-79	0.11870936960167092	8.89729436058899E-4	0.0	7.785132565515366E-4	6.811990994825945E-4
80-81	0.13896461629444928	9.453375258125802E-4	0.0	7.785132565515366E-4	7.646112341131164E-4
82-83	0.1630151151129164	9.731415706894208E-4	0.0	7.785132565515366E-4	8.202193238667975E-4
84-85	0.19188961571751534	9.731415706894208E-4	0.0	7.924152789899569E-4	8.341213463052177E-4
86-87	0.22902191765053598	9.87043593127841E-4	0.0	8.063173014283772E-4	8.341213463052177E-4
88	0.25902248207264694	0.0010009456155662614	0.0	8.063173014283772E-4	8.619253911820584E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	1855	0.0	82.891014	1
CGTGTGG	1755	0.0	51.677315	3
AACGTGT	1540	0.0	48.24363	1
ACGTGTG	1880	0.0	44.491997	2
GTGGAAT	151040	0.0	43.051693	6
TGGAATC	152850	0.0	42.62181	7
TCGAGTG	1015	0.0	42.593323	2
AGTGGAA	150860	0.0	42.4687	5
GGAATCA	154510	0.0	42.14261	8
GAATCAC	154465	0.0	42.130558	9
AACGAGT	154230	0.0	40.000793	1
ACGAGTG	158280	0.0	39.9731	2
CGAGTGG	161020	0.0	39.54679	3
GAGTGGA	163400	0.0	39.249733	4
ATCGAGT	935	0.0	39.22714	1
AGTACTT	35095	0.0	38.26968	20-21
GAGTACT	34500	0.0	38.003445	20-21
GTACTTT	34505	0.0	37.154057	22-23
TACTTTT	36920	0.0	35.780224	22-23
CATGGGG	18515	0.0	34.66132	24-25
>>END_MODULE