##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2132709_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4455776 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.91874411999167 33.0 31.0 34.0 25.0 34.0 2 31.66294243696272 34.0 31.0 34.0 28.0 34.0 3 31.86361612432941 34.0 31.0 34.0 28.0 34.0 4 35.799484534231524 37.0 35.0 37.0 35.0 37.0 5 35.467200550476505 37.0 35.0 37.0 33.0 37.0 6 35.82752970526346 37.0 36.0 37.0 35.0 37.0 7 36.01239739161035 37.0 35.0 37.0 35.0 37.0 8 36.16234501016209 37.0 37.0 37.0 35.0 37.0 9 38.09526713192046 39.0 39.0 39.0 37.0 39.0 10-11 38.176156745761006 39.0 39.0 39.0 37.0 39.0 12-13 38.1693807094432 39.0 39.0 39.0 37.0 39.0 14-15 39.72365947031449 41.0 40.0 41.0 38.0 41.0 16-17 39.67263693237721 41.0 40.0 41.0 38.0 41.0 18-19 39.645767875225324 41.0 40.0 41.0 37.0 41.0 20-21 39.54399402034572 41.0 40.0 41.0 37.0 41.0 22-23 39.448080087508885 41.0 39.5 41.0 37.0 41.0 24-25 39.32457331786876 41.0 39.0 41.0 36.0 41.0 26-27 39.29134790438299 41.0 39.0 41.0 36.5 41.0 28-29 39.16561817739491 41.0 39.0 41.0 36.0 41.0 30-31 39.041440593063925 41.0 39.0 41.0 35.5 41.0 32-33 38.92137912677836 41.0 39.0 41.0 35.0 41.0 34-35 38.815472658410116 41.0 39.0 41.0 35.0 41.0 36-37 38.61687863124179 40.5 38.5 41.0 35.0 41.0 38-39 38.26762061198768 40.0 38.0 41.0 35.0 41.0 40-41 38.1321181764972 40.0 38.0 41.0 35.0 41.0 42-43 38.04891269219996 40.0 37.5 41.0 34.5 41.0 44-45 37.894207092097986 40.0 37.0 41.0 34.0 41.0 46-47 37.80160964105916 40.0 37.0 41.0 34.0 41.0 48-49 37.65658473406204 40.0 37.0 41.0 33.0 41.0 50-51 37.4180938404444 40.0 36.5 41.0 33.0 41.0 52-53 36.96376983492887 40.0 36.0 41.0 32.5 41.0 54-55 36.13140662816084 40.0 35.0 41.0 29.5 41.0 56-57 35.61068756598178 40.0 35.0 41.0 26.5 41.0 58-59 35.381627465114946 39.5 35.0 41.0 26.0 41.0 60-61 35.12564803526928 39.0 35.0 41.0 25.0 41.0 62-63 34.89538242048074 39.0 35.0 41.0 24.0 41.0 64-65 34.54396203938438 38.5 35.0 40.5 23.0 41.0 66-67 34.11316188246447 37.5 34.5 40.0 21.0 41.0 68-69 33.85221216237082 37.0 34.0 40.0 22.0 41.0 70-71 33.56086178928204 37.0 34.0 39.5 21.0 41.0 72-73 33.21618759560624 36.0 34.0 39.0 20.0 41.0 74-75 32.84335770020755 36.0 34.0 39.0 18.5 40.5 76-77 32.44975611431095 35.0 34.0 38.0 17.0 40.0 78-79 32.08800790255165 35.0 33.5 37.0 14.0 39.0 80-81 31.72223244615528 35.0 33.0 37.0 9.0 39.0 82-83 31.28375775622473 35.0 32.5 36.0 7.0 38.5 84-85 30.930489212204563 35.0 32.0 36.0 4.0 37.0 86-87 30.854620609294543 35.0 32.5 36.0 2.0 37.0 88-89 30.920627742507705 35.0 33.0 35.0 2.0 37.0 90-91 30.7541828853156 35.0 33.0 35.0 2.0 36.0 92-93 30.620317650617984 35.0 33.0 35.0 2.0 36.0 94-95 30.39652161598788 35.0 33.0 35.0 2.0 36.0 96-97 30.12473259427763 35.0 32.5 35.0 2.0 36.0 98-99 30.024011754630394 35.0 33.0 35.0 2.0 35.5 100 30.00623595081979 35.0 33.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.8963329169452088 1101 2 1.3704828184726736 1101 3 1.2530843202598874 1101 4 0.6475963467329464 1101 5 0.9871751407374063 1101 6 0.6544973044552975 1101 7 0.44202218090151035 1101 8 0.36905425280956905 1101 9 0.37908939693443244 1101 10-11 0.2397778192871769 1101 12-13 0.25788560449449704 1101 14-15 0.3510700434108145 1101 16-17 0.37415847833675997 1101 18-19 0.31789415259176224 1101 20-21 0.40581199443298743 1101 22-23 0.40308561529520404 1101 24-25 0.38338094740061734 1101 26-27 0.4265244824895973 1101 28-29 0.5110121876103406 1101 30-31 0.5633651687652872 1101 32-33 0.5320265289351624 1101 34-35 0.4731786958561486 1101 36-37 0.5337851520617818 1101 38-39 0.5048448567500188 1101 40-41 0.4673028289857726 1101 42-43 0.5089670050336608 1101 44-45 0.5287155109110344 1101 46-47 0.5380507595253476 1101 48-49 0.4694244355375403 1101 50-51 0.5063242077854753 1101 52-53 0.56244913422384 1101 54-55 0.4771783238603007 1101 56-57 0.5149522730497864 1101 58-59 0.5500213861101244 1101 60-61 0.49771297009417026 1101 62-63 0.5440436628532197 1101 64-65 0.5152934539764331 1101 66-67 0.5819427801486938 1101 68-69 0.6298115938158801 1101 70-71 0.5647064408177584 1101 72-73 0.6225934437701284 1101 74-75 0.5418060986785704 1101 76-77 0.5782799916126322 1101 78-79 0.5629627609030265 1101 80-81 0.6105205748893319 1101 82-83 0.5958963302509908 1101 84-85 0.5612882956519059 1101 86-87 0.6336759484826544 1101 88-89 0.5980447220369527 1101 90-91 0.6076152778823172 1101 92-93 0.6178508525553781 1101 94-95 0.6393940508425011 1101 96-97 0.6884941527025674 1101 98-99 0.6407306730357476 1101 100 0.6783463968811816 1102 1 1.3562054000160622 1102 2 1.1836472173782724 1102 3 1.1281673490827941 1102 4 0.5778422605804678 1102 5 0.9246887442434044 1102 6 0.5584182562008451 1102 7 0.3564891295336139 1102 8 0.3170003325311157 1102 9 0.2997612173253046 1102 10-11 0.21739021270852277 1102 12-13 0.18897178349562438 1102 14-15 0.30442349381488754 1102 16-17 0.2901727275884909 1102 18-19 0.2673349755554213 1102 20-21 0.3095238184147391 1102 22-23 0.3291576246151635 1102 24-25 0.3244281776308995 1102 26-27 0.3041596861392293 1102 28-29 0.3898616380633868 1102 30-31 0.4370073991077845 1102 32-33 0.4315248263291238 1102 34-35 0.40675792263429855 1102 36-37 0.4800765721107112 1102 38-39 0.4428472683501852 1102 40-41 0.36681838707126246 1102 42-43 0.3362893331459347 1102 44-45 0.39328200435870286 1102 46-47 0.3115706552267028 1102 48-49 0.41111002571648925 1102 50-51 0.394177332766354 1102 52-53 0.4117238383335575 1102 54-55 0.38065988287207375 1102 56-57 0.2986223122841949 1102 58-59 0.3351447510880661 1102 60-61 0.3200083156242357 1102 62-63 0.30276886081050947 1102 64-65 0.3392386849452933 1102 66-67 0.43225120789556115 1102 68-69 0.41890234710428587 1102 70-71 0.3821928679158191 1102 72-73 0.3503239484719245 1102 74-75 0.35535125293324654 1102 76-77 0.30660364386069716 1102 78-79 0.34166388366408995 1102 80-81 0.3827249039360545 1102 82-83 0.31616070854985523 1102 84-85 0.31977290486862486 1102 86-87 0.2823297165569052 1102 88-89 0.2746471405740394 1102 90-91 0.3107674555654256 1102 92-93 0.30936748790388435 1102 94-95 0.28827522750751555 1102 96-97 0.3368219671032051 1102 98-99 0.37944097701570456 1102 100 0.3986430522651503 1103 1 1.8433829433956426 1103 2 1.3279897622427228 1103 3 1.236353997930955 1103 4 0.6393965539918582 1103 5 0.9598786226957401 1103 6 0.593938386869894 1103 7 0.4197975912026095 1103 8 0.2798902184078287 1103 9 0.29275432856369576 1103 10-11 0.24288376145486978 1103 12-13 0.23321126209220466 1103 14-15 0.315139840172705 1103 16-17 0.28850952121200635 1103 18-19 0.28564692403024594 1103 20-21 0.39076585046684187 1103 22-23 0.3866372871241808 1103 24-25 0.37381548088489325 1103 26-27 0.33947965029010163 1103 28-29 0.41965583697306386 1103 30-31 0.3672581176814589 1103 32-33 0.34637218908420664 1103 34-35 0.34534315448699715 1103 36-37 0.4602516680973068 1103 38-39 0.4564110490552906 1103 40-41 0.43923388174692235 1103 42-43 0.4727728032999394 1103 44-45 0.5116602816910216 1103 46-47 0.4854569190678646 1103 48-49 0.4104526368700263 1103 50-51 0.42616966444511206 1103 52-53 0.43663352608545836 1103 54-55 0.3807041772380302 1103 56-57 0.4135937549467883 1103 58-59 0.4066347440879383 1103 60-61 0.42389520258279134 1103 62-63 0.36185016039474505 1103 64-65 0.34159864262634443 1103 66-67 0.4110068954989714 1103 68-69 0.472306715820082 1103 70-71 0.4263105336915629 1103 72-73 0.45037723163768817 1103 74-75 0.44784487592336575 1103 76-77 0.40207517137331905 1103 78-79 0.4016833859126123 1103 80-81 0.5132347067658038 1103 82-83 0.5254674307932881 1103 84-85 0.48539937648629206 1103 86-87 0.44129479811899586 1103 88-89 0.41192044516442294 1103 90-91 0.44940522814613715 1103 92-93 0.44037675246627117 1103 94-95 0.41869189417418085 1103 96-97 0.4115119065414987 1103 98-99 0.47576154837457807 1103 100 0.5005915005702732 1104 1 1.8270731591118157 1104 2 1.3561110108334091 1104 3 1.211275623893144 1104 4 0.6229055107350732 1104 5 0.9142150237355935 1104 6 0.5782398541778804 1104 7 0.3320140556311415 1104 8 0.24969167760465893 1104 9 0.2406204610431928 1104 10-11 0.17910753454123807 1104 12-13 0.1900778112823005 1104 14-15 0.30061864166840024 1104 16-17 0.29710048933922906 1104 18-19 0.2723349593664892 1104 20-21 0.2849556905351349 1104 22-23 0.2531366696624815 1104 24-25 0.28614796018117516 1104 26-27 0.26188177399332346 1104 28-29 0.2516571200564428 1104 30-31 0.27767457734468337 1104 32-33 0.26856898649593575 1104 34-35 0.2686030075128656 1104 36-37 0.25460205834526306 1104 38-39 0.25403744620178514 1104 40-41 0.2912559674088371 1104 42-43 0.28708709130273036 1104 44-45 0.32048970510955144 1104 46-47 0.3189617441913626 1104 48-49 0.2804980517353428 1104 50-51 0.20519092175528897 1104 52-53 0.3051486067508975 1104 54-55 0.32636363190478335 1104 56-57 0.4010382188805366 1104 58-59 0.30961901554396576 1104 60-61 0.32020866983805263 1104 62-63 0.3378392017170242 1104 64-65 0.27662346022673745 1104 66-67 0.2894801809021388 1104 68-69 0.26412055770932596 1104 70-71 0.33649986833349743 1104 72-73 0.3370747975813799 1104 74-75 0.3148403950170291 1104 76-77 0.3567754084218393 1104 78-79 0.355548871020531 1104 80-81 0.30324910153167295 1104 82-83 0.3156724503817294 1104 84-85 0.3140117175076469 1104 86-87 0.291909484278122 1104 88-89 0.2547018591618446 1104 90-91 0.23288043487920973 1104 92-93 0.25470452526469956 1104 94-95 0.2742337903189487 1104 96-97 0.335935849759359 1104 98-99 0.37491759534222524 1104 100 0.3466644087033295 1105 1 1.5992204631888463 1105 2 1.29967604633147 1105 3 1.1798211792343238 1105 4 0.5744395646885962 1105 5 0.9486942354725869 1105 6 0.6339977144532511 1105 7 0.34903442802013984 1105 8 0.3049378301743957 1105 9 0.2468195558833628 1105 10-11 0.2387953461641814 1105 12-13 0.2263281196595699 1105 14-15 0.28290093037208663 1105 16-17 0.26230872754247514 1105 18-19 0.2817405301842655 1105 20-21 0.3443709082614035 1105 22-23 0.3561343105382022 1105 24-25 0.3790093212151646 1105 26-27 0.3727372647704783 1105 28-29 0.43092754495663144 1105 30-31 0.3759920074326999 1105 32-33 0.3541743571907645 1105 34-35 0.28481867255265314 1105 36-37 0.3190488200570911 1105 38-39 0.3643176293256474 1105 40-41 0.36781870854336063 1105 42-43 0.4550602104580008 1105 44-45 0.45783029132145003 1105 46-47 0.4837963861603143 1105 48-49 0.49523786807628767 1105 50-51 0.4462255957624848 1105 52-53 0.4471064636293889 1105 54-55 0.5452555534162187 1105 56-57 0.6368051065605869 1105 58-59 0.6179944382759004 1105 60-61 0.60712669213261 1105 62-63 0.6971287958002819 1105 64-65 0.5626047854248171 1105 66-67 0.5679007125537154 1105 68-69 0.6121187233915322 1105 70-71 0.5529891536668146 1105 72-73 0.5797915893971819 1105 74-75 0.5697431689644787 1105 76-77 0.5904433756392109 1105 78-79 0.5027853036121002 1105 80-81 0.5219640184625653 1105 82-83 0.5691916535947392 1105 84-85 0.5761332015944021 1105 86-87 0.5703236987014613 1105 88-89 0.5738274570227375 1105 90-91 0.5889529468840813 1105 92-93 0.5942955721027339 1105 94-95 0.6046048853218764 1105 96-97 0.6449411380247447 1105 98-99 0.6319305068036414 1105 100 0.6228981285524604 1106 1 1.625377796228097 1106 2 1.2939759959584975 1106 3 1.1400114401477168 1106 4 0.6437227268806893 1106 5 0.9770779740794922 1106 6 0.6276806674949285 1106 7 0.3983244175052718 1106 8 0.3151592679219348 1106 9 0.26010924717796513 1106 10-11 0.24280479319278214 1106 12-13 0.19445305902741694 1106 14-15 0.27289877545737085 1106 16-17 0.29437349839285787 1106 18-19 0.28865983481267676 1106 20-21 0.3287618045784413 1106 22-23 0.33808962480102167 1106 24-25 0.33742966825453635 1106 26-27 0.2970700314691328 1106 28-29 0.40724754633288995 1106 30-31 0.40941913271438324 1106 32-33 0.41247761989846765 1106 34-35 0.3852450087099655 1106 36-37 0.43320630408729244 1106 38-39 0.3870473233538192 1106 40-41 0.3752704742583006 1106 42-43 0.3895330783660853 1106 44-45 0.3938388973314275 1106 46-47 0.41685303861711276 1106 48-49 0.3879773551895269 1106 50-51 0.4376351777540535 1106 52-53 0.40541046328285546 1106 54-55 0.35806014483003423 1106 56-57 0.16132999810041326 1106 58-59 0.1649994812123481 1106 60-61 0.11443205207896767 1106 62-63 0.18179704201106972 1106 64-65 0.13306848259973236 1106 66-67 0.10205594498948756 1106 68-69 0.12763952391083677 1106 70-71 0.16693225993728333 1106 72-73 0.22857386590368378 1106 74-75 0.23725141505583736 1106 76-77 0.2226879375600248 1106 78-79 0.1712902984693514 1106 80-81 0.234770863366478 1106 82-83 0.19745878179008258 1106 84-85 0.15782528641302207 1106 86-87 0.19257468366146568 1106 88-89 0.17709584466075867 1106 90-91 0.1520963082373079 1106 92-93 0.19993921176554608 1106 94-95 0.15351986182752242 1106 96-97 0.13398870221047332 1106 98-99 0.1258036795276638 1106 100 0.07269525046409697 1107 1 1.659270223793147 1107 2 1.2935018061214194 1107 3 1.2236181758983946 1107 4 0.6579101513075472 1107 5 1.0056795137252124 1107 6 0.6521386606278412 1107 7 0.4217879686417376 1107 8 0.3407353609075088 1107 9 0.3225493604298606 1107 10-11 0.22232789094949368 1107 12-13 0.16185177439736975 1107 14-15 0.2568768560252721 1107 16-17 0.2456911237256918 1107 18-19 0.20103142291229403 1107 20-21 0.2862405748895114 1107 22-23 0.2861629043182816 1107 24-25 0.24653860019257223 1107 26-27 0.2847605289633037 1107 28-29 0.2854647245449442 1107 30-31 0.3209504386394286 1107 32-33 0.3262493880123998 1107 34-35 0.36461092165648523 1107 36-37 0.291334145227367 1107 38-39 0.3103472776214957 1107 40-41 0.3521131344403656 1107 42-43 0.3094311582593292 1107 44-45 0.33337130563555206 1107 46-47 0.36859267273835883 1107 48-49 0.41909435596309663 1107 50-51 0.4048741645736271 1107 52-53 0.32367750155253816 1107 54-55 0.3858272918617871 1107 56-57 0.36893345751373374 1107 58-59 0.37495965861599245 1107 60-61 0.4132384283510291 1107 62-63 0.40708208528627665 1107 64-65 0.4518493380535986 1107 66-67 0.44504346746263224 1107 68-69 0.43216640519461436 1107 70-71 0.5014219026980768 1107 72-73 0.4989729205306688 1107 74-75 0.3650550406968449 1107 76-77 0.4592857726033728 1107 78-79 0.4249261740242716 1107 80-81 0.42329448389616786 1107 82-83 0.46459064277298623 1107 84-85 0.4935718805740805 1107 86-87 0.41178740661570856 1107 88-89 0.40732073970970717 1107 90-91 0.4075566642527697 1107 92-93 0.342795660577746 1107 94-95 0.39485778483203404 1107 96-97 0.37028062385080673 1107 98-99 0.3218236794671654 1107 100 0.24008242962856485 1108 1 0.7021574575992524 1108 2 0.8702075705334096 1108 3 0.986030753309791 1108 4 0.50323569552625 1108 5 0.8641291861910148 1108 6 0.5033951556479366 1108 7 0.3259044172126977 1108 8 0.2559065565785943 1108 9 0.2272244618122059 1108 10-11 0.1361920180594538 1108 12-13 0.126547343740242 1108 14-15 0.1523306406852427 1108 16-17 0.2006962541749573 1108 18-19 0.18048240115545156 1108 20-21 0.17167031999264992 1108 22-23 0.18761020836928566 1108 24-25 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-0.865739523648358 2215 60-61 -0.8773708541313283 2215 62-63 -0.888356126193905 2215 64-65 -0.9308987835714149 2215 66-67 -0.9382485280131689 2215 68-69 -0.875180409851815 2215 70-71 -0.8522455423958064 2215 72-73 -0.8801460608431029 2215 74-75 -0.9295095518999048 2215 76-77 -0.8507652339107565 2215 78-79 -0.8935635195533891 2215 80-81 -0.9769492110969829 2215 82-83 -1.0051905088037891 2215 84-85 -0.9845685988372708 2215 86-87 -0.9812270573188009 2215 88-89 -0.9229986176918104 2215 90-91 -0.861588578123321 2215 92-93 -0.8581316487710495 2215 94-95 -0.9152828106626885 2215 96-97 -0.9916821583106277 2215 98-99 -0.9872184904864412 2215 100 -0.9771655189201134 2216 1 -6.153719575099444 2216 2 -6.201126148044914 2216 3 -5.576277986746415 2216 4 -3.1223577067354285 2216 5 -4.094385106984475 2216 6 -4.289408696303202 2216 7 -2.1426803969925103 2216 8 -1.3805165666384127 2216 9 -1.0723295256438945 2216 10-11 -0.9706821677344948 2216 12-13 -0.8503858252161862 2216 14-15 -1.0761900760325318 2216 16-17 -0.9895865985246246 2216 18-19 -0.987560658144865 2216 20-21 -1.0218273723267473 2216 22-23 -1.053418774487504 2216 24-25 -1.5108516429036385 2216 26-27 -1.3492523861817887 2216 28-29 -2.024872357865952 2216 30-31 -1.5415078509999205 2216 32-33 -1.364964603333192 2216 34-35 -1.4035586675218497 2216 36-37 -1.4917045632163521 2216 38-39 -1.396017998842396 2216 40-41 -1.3978673255165788 2216 42-43 -1.4148199951956357 2216 44-45 -1.409796838623869 2216 46-47 -1.3920956103168223 2216 48-49 -1.442291349987336 2216 50-51 -1.5271214148380494 2216 52-53 -1.5052460477177547 2216 54-55 -1.2783099734664134 2216 56-57 -1.2459674278771473 2216 58-59 -1.172614753302014 2216 60-61 -1.2316049422385191 2216 62-63 -1.280203313128375 2216 64-65 -1.39678663038206 2216 66-67 -1.4202486806813397 2216 68-69 -1.415954362794544 2216 70-71 -1.338330374941961 2216 72-73 -1.4067838050898942 2216 74-75 -1.4203124573072756 2216 76-77 -1.4316560759473553 2216 78-79 -1.3910745989019162 2216 80-81 -1.3803996967978556 2216 82-83 -1.3930978078887684 2216 84-85 -1.3455852089612108 2216 86-87 -1.325942631202004 2216 88-89 -1.2241705860976815 2216 90-91 -1.2597274115439632 2216 92-93 -1.302715826858929 2216 94-95 -1.3608784026569474 2216 96-97 -1.4569540873306899 2216 98-99 -1.5422719673767418 2216 100 -1.5649040648479513 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4627.0 3 1820.0 4 2323.0 5 3058.0 6 2433.0 7 2285.0 8 2420.0 9 2993.0 10 3741.0 11 9514.0 12 6848.0 13 9568.0 14 8682.0 15 9313.0 16 11082.0 17 14003.0 18 18807.0 19 29218.0 20 63116.0 21 98492.0 22 44975.0 23 26608.0 24 25123.0 25 25833.0 26 27066.0 27 28546.0 28 32782.0 29 38271.0 30 45706.0 31 56537.0 32 72331.0 33 98051.0 34 142809.0 35 268692.0 36 441314.0 37 907252.0 38 1305980.0 39 559239.0 40 4318.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 14.699588765688512 43.395605701005955 25.261661388414886 16.643144144890652 2 17.454468088162418 41.28270810740935 23.750745100292296 17.512078704135938 3 18.4623325908512 23.4463589386807 20.499553949649613 37.59175452081849 4 35.311145154390964 24.450004780322846 22.179239214985706 18.059610850300487 5 17.358958434662345 39.979880463377796 23.063668547467763 19.597492554492096 6 37.73962930511317 21.840784876309353 23.31193419923369 17.107651619343788 7 18.756530848947524 22.19175739534483 40.92063873946985 18.1310730162378 8 36.944047456604636 22.653113621510595 22.66018309717544 17.74265582470932 9 36.79516205482502 23.963233340275632 22.852966576416765 16.38863802848258 10-11 16.676747215299873 42.776319994541915 22.97854515128229 17.568387638875922 12-13 16.563691711612076 23.267686705974448 32.56286895930137 27.60575262311211 14-15 16.942073734539143 32.81542037197628 22.728999804298606 27.513506089185974 16-17 27.045200864991465 22.798007929724324 32.43021150233074 17.726579702953465 18-19 17.37008323578205 23.27918863066725 32.3940431475909 26.956684985959793 20-21 27.208638854376883 32.896952180720035 22.977838203715805 16.916570761187277 22-23 26.549738995992726 23.23303165364405 32.672052860843834 17.54517648951939 24-25 16.951201570965285 32.744984434771986 22.957030858551292 27.34678313571144 26-27 17.333371937700186 28.610145160274897 36.577783478655206 17.478699423369715 28-29 28.8761492108852 22.920557965877798 30.868767464206968 17.334525359030035 30-31 24.31954887218045 26.207126024015263 31.47231511614858 18.001009987655706 32-33 19.06967025656886 27.26000898635125 32.18827303605358 21.482047721026312 34-35 20.969274418043955 27.034810338636788 32.45298326307092 19.54293198024833 36-37 21.272216586364106 25.13291284020086 34.641475989710436 18.953394583724595 38-39 23.61900917972413 25.21560347704205 32.47445122507626 18.690936118157563 40-41 18.781866700182015 29.94457623657048 32.29213563841128 18.98142142483622 42-43 18.790391786511975 25.188438425716708 34.63502024071145 21.386149547059865 44-45 18.74975030558108 27.588259402089037 32.03931772486974 21.62267256746014 46-47 21.40534123030759 25.03095462793289 34.16710898250898 19.396595159250534 48-49 19.246808207394555 25.453056955394864 34.043079450708106 21.257055386502476 50-51 21.54417932135682 27.73692908562798 31.863165722142757 18.855725870872444 52-53 21.23280884437034 25.648541560692156 33.60323659480655 19.51541300013096 54-55 19.445132460212335 28.326319313411265 30.29214901497528 21.936399211401127 56-57 19.868184057520498 28.813173963153034 31.23783092860325 20.080811050723216 58-59 24.677518224156735 27.080096956082684 28.479502551280937 19.762882268479647 60-61 22.10257890447565 29.418220822777837 28.42519412876769 20.054006143978825 62-63 19.79817677503422 29.395042025651907 28.16999051334113 22.636790685972738 64-65 21.786428020465586 29.298346994003506 28.45161476405084 20.463610221480067 66-67 22.14993644991708 26.948018209236547 30.744650312947407 20.157395027898968 68-69 24.118283522799196 27.228703830882083 28.903418644589696 19.749594001729026 70-71 19.4494655281329 31.42794008905414 28.93245120870175 20.190143174111206 72-73 19.24745420269353 27.166378317100133 31.122855319440706 22.46331216076563 74-75 19.53615810934939 29.201109440852356 28.622081419222418 22.640651030575835 76-77 21.99216666436611 26.885706491079286 30.62694852849171 20.495178316062894 78-79 20.052768879088358 27.295959137058574 30.257292336312997 22.39397964754007 80-81 22.217336768712418 29.383825729813246 28.3192195723687 20.079617929105638 82-83 21.974806221994974 27.454482117297278 30.184208346735193 20.386503313972554 84-85 19.919132838284753 29.629499119618536 27.910258323785115 22.541109718311596 86-87 19.96099819131806 29.873351861586677 29.771840765958174 20.393809181137097 88-89 24.336242015919392 27.69799324583454 27.661112346335663 20.3046523919104 90-91 22.016110948122584 29.985254713654353 27.653079708721673 20.345554629501393 92-93 20.04211212001225 29.882837577092946 27.316626756560925 22.75842354633388 94-95 21.89771978709484 30.1565479109551 27.17877098106724 20.766961320882817 96-97 22.122348966015522 27.942545457238865 29.30057559361423 20.63452998313138 98-99 24.193610115568042 28.30516401310933 27.15663285330655 20.34459301801607 100 19.949221624198717 32.084988572508564 27.31904451716099 20.646745286131726 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 44748.0 1 23960.5 2 2375.0 3 1361.0 4 1091.0 5 1010.5 6 1104.5 7 1361.5 8 1265.0 9 1150.0 10 1304.5 11 2008.0 12 20723.5 13 24642.5 14 10299.0 15 10988.0 16 12864.5 17 14572.5 18 16128.0 19 17849.0 20 20079.5 21 22860.5 22 25986.0 23 30055.5 24 34326.5 25 38610.5 26 44978.0 27 51908.5 28 57331.5 29 61986.5 30 67220.5 31 73236.0 32 79549.5 33 87161.0 34 94912.0 35 101103.0 36 107023.5 37 112433.0 38 116754.0 39 118876.5 40 120271.0 41 122950.5 42 124531.5 43 125245.0 44 126241.5 45 128096.0 46 131515.5 47 142417.5 48 151183.5 49 189164.5 50 354607.5 51 349873.5 52 163944.0 53 99838.5 54 85340.0 55 77961.0 56 70287.5 57 63033.0 58 59429.5 59 52094.0 60 40913.5 61 32178.5 62 25035.5 63 19421.5 64 14889.0 65 11517.0 66 8780.0 67 6720.0 68 5462.0 69 4256.5 70 3102.0 71 2500.0 72 2145.5 73 1655.0 74 1285.0 75 1025.0 76 693.0 77 469.5 78 348.0 79 222.5 80 127.0 81 71.5 82 35.0 83 21.5 84 16.5 85 9.0 86 5.5 87 4.5 88 3.0 89 4.5 90 4.0 91 2.0 92 1.0 93 1.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05828389937016583 2 0.0 3 2.2442779888396545E-5 4 2.2442779888396546E-4 5 0.0090893258548006 6 0.0032317603039291026 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.7954223910717236E-4 16-17 2.1320640893976719E-4 18-19 0.0 20-21 0.0 22-23 3.3664169832594814E-5 24-25 0.0038489367508600073 26-27 0.007215353734119489 28-29 0.0012455742838060082 30-31 0.006194207249197446 32-33 0.002917561385491551 34-35 0.02615705995992617 36-37 0.026145838569981976 38-39 0.0227233146370015 40-41 0.0023789346681700337 42-43 0.006182985859253247 44-45 0.00740611736317086 46-47 0.0013690095731921893 48-49 0.009055661684968005 50-51 0.009987037050336461 52-53 0.002367713278225835 54-55 0.01195078029057116 56-57 0.007204132344175291 58-59 0.012635285077167255 60-61 0.008012072420157565 62-63 0.0010099250949778444 64-65 0.013230018744209765 66-67 0.005274053273773188 68-69 0.002064735749732482 70-71 0.008898562225749229 72-73 0.005532145242489748 74-75 0.008494592187758093 76-77 0.007417338753115059 78-79 0.015440632563216823 80-81 0.008359935508427712 82-83 0.012321086158729703 84-85 0.007204132344175291 86-87 0.013420782373261133 88-89 0.020097509390059106 90-91 0.017797124451498458 92-93 0.01714628383473496 94-95 0.006081993349755463 96-97 0.010379785698383402 98-99 0.017539032482781897 100 0.016383229318529476 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4455776.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.07514976010204 #Duplication Level Percentage of deduplicated Percentage of total 1 88.3210988841843 53.05903242444153 2 8.724658371654229 10.482703165657117 3 1.3699703111385915 2.4690351482563346 4 0.4064137341897294 0.976614637840412 5 0.21889458172777923 0.6575062389485617 6 0.14171060036792074 0.5107971323818086 7 0.11572602779963889 0.48665809158435275 8 0.08934211562249289 0.4293792780724491 9 0.06335581305382466 0.342549896164339 >10 0.4910994071751459 6.425019094327656 >50 0.039038612609676615 1.557160655277559 >100 0.01418454967544632 1.7405010035422928 >500 0.0013145390129767017 0.5277302006526972 >1k 0.0015398885095820553 2.519742985598897 >5k 0.0014272137405882453 6.203768557272461 >10k+ 2.2534953798761768E-4 11.611801489981536 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 274480 6.160094223767083 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG 160316 3.5979367005881806 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 44717 1.0035737882694284 No Hit GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT 14629 0.32831542698735305 No Hit AGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTC 10464 0.23484124875218143 No Hit TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG 10029 0.22507863950072893 No Hit CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA 9521 0.21367770731742347 No Hit TCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGT 9432 0.21168029990735623 No Hit TACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACT 9184 0.20611449049503386 No Hit TCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCA 9127 0.20483525204139527 No Hit CCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGAT 8991 0.20178303397657332 No Hit TCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGA 8983 0.20160349173746614 No Hit AACGAGTGGAATCACTGCTGAGTACATGGTAACGAGTGGAATCACTGCTG 8909 0.19994272602572483 No Hit CCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATT 8875 0.19917967150951932 No Hit ACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTC 8735 0.19603768232514382 No Hit TCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACT 8516 0.191122713529585 No Hit ACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGA 8481 0.1903372162334911 No Hit CTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCG 8448 0.189596604497174 No Hit TGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCA 8397 0.18845202272286576 No Hit CCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTC 8093 0.18162941763679324 No Hit CGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAG 8047 0.18059704976192698 No Hit ATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTA 7822 0.17554742428703776 No Hit ACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAG 7677 0.17229322120322027 No Hit GAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGT 7385 0.16573992947580848 No Hit GATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGT 7332 0.16455046214172347 No Hit GTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCAC 7311 0.16407916376406714 No Hit CAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTT 7306 0.16396694986462515 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA 7156 0.16060053288136567 No Hit TGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATG 6885 0.1545185395316102 No Hit CTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGC 6661 0.14949135683660938 No Hit GTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCAT 6501 0.14590051205446594 No Hit ATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCC 6484 0.1455189847963632 No Hit CGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAG 5700 0.1279238453638603 No Hit TTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTAC 5688 0.12765453200519952 No Hit TGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTG 5570 0.12500628397836877 No Hit CTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACG 5570 0.12500628397836877 No Hit TGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGA 5546 0.12446765726104723 No Hit ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 5453 0.12238047873142635 No Hit TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 5385 0.12085436969901538 No Hit ATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGG 5301 0.11896917618839009 No Hit CTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGT 5269 0.1182510072319614 No Hit ATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCAC 5139 0.11533344584646985 No Hit ACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATC 5055 0.11344825233584453 No Hit GCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCC 5016 0.11257298392019707 No Hit ACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAAC 4967 0.11147328770566563 No Hit TGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACA 4658 0.10453846872015111 No Hit CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 4595 0.10312457358718212 No Hit CAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCC 4506 0.10112716617711483 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.003141989184375516 0.0 0.0 2.2442779888396545E-5 0.0 2 0.0031644319642639127 0.0 0.0 4.488555977679309E-5 0.0 3 0.0031644319642639127 2.2442779888396545E-5 0.0 4.488555977679309E-5 0.0 4 0.0031644319642639127 4.488555977679309E-5 0.0 6.732833966518963E-5 2.2442779888396545E-5 5 0.0031644319642639127 8.977111955358618E-5 0.0 6.732833966518963E-5 2.2442779888396545E-5 6 0.0031644319642639127 1.3465667933037926E-4 0.0 1.1221389944198272E-4 2.2442779888396545E-5 7 0.0031644319642639127 1.3465667933037926E-4 0.0 1.1221389944198272E-4 2.2442779888396545E-5 8 0.0031644319642639127 1.570994592187758E-4 0.0 1.3465667933037926E-4 2.2442779888396545E-5 9 0.0031868747441523093 1.7954223910717236E-4 0.0 1.570994592187758E-4 2.2442779888396545E-5 10-11 0.0032205389139849045 2.019850189955689E-4 0.0 1.570994592187758E-4 2.2442779888396545E-5 12-13 0.003265424473761697 2.2442779888396543E-4 0.0 2.1320640893976716E-4 2.2442779888396545E-5 14-15 0.0034561881028130678 2.2442779888396543E-4 0.0 2.580919687165602E-4 2.2442779888396545E-5 16-17 0.0038264939709716107 2.4687057877236196E-4 0.0 3.8152725810274127E-4 2.2442779888396545E-5 18-19 0.004039700379911378 2.805347486049568E-4 0.0 4.039700379911378E-4 2.2442779888396545E-5 20-21 0.004510998757567705 3.141989184375516E-4 0.0 4.039700379911378E-4 2.2442779888396545E-5 22-23 0.004858861845837852 3.927486480469395E-4 0.0 4.264128178795343E-4 2.2442779888396545E-5 24-25 0.005487259682712955 4.4885559776793087E-4 0.0 4.264128178795343E-4 2.2442779888396545E-5 26-27 0.0061268789095322565 4.937411575447239E-4 0.0 4.4885559776793087E-4 2.2442779888396545E-5 28-29 0.006979704545291326 4.937411575447239E-4 0.0 4.937411575447239E-4 2.2442779888396545E-5 30-31 0.007911079910659782 5.161839374331204E-4 0.0 4.937411575447239E-4 2.2442779888396545E-5 32-33 0.009179096974354187 5.498481072657153E-4 0.0 4.937411575447239E-4 2.2442779888396545E-5 34-35 0.010671541836932558 5.610694972099136E-4 0.0 5.38626717321517E-4 2.2442779888396545E-5 36-37 0.012018108630236349 5.610694972099136E-4 0.0 5.38626717321517E-4 2.2442779888396545E-5 38-39 0.013443225153149531 5.835122770983101E-4 0.0 5.498481072657153E-4 2.2442779888396545E-5 40-41 0.015721167311821778 6.059550569867067E-4 0.0 5.722908871541119E-4 2.2442779888396545E-5 42-43 0.017763460281665866 6.283978368751032E-4 0.0 5.835122770983101E-4 2.2442779888396545E-5 44-45 0.020097509390059103 6.620620067076981E-4 0.0 6.171764469309049E-4 2.2442779888396545E-5 46-47 0.02215102374984739 7.518331262612842E-4 0.0 6.283978368751032E-4 2.2442779888396545E-5 48-49 0.02437285895879865 7.967186860380773E-4 0.0 6.283978368751032E-4 2.2442779888396545E-5 50-51 0.027817825671667518 8.079400759822756E-4 0.0 6.732833966518963E-4 2.2442779888396545E-5 52-53 0.03154332713314134 8.079400759822756E-4 0.0 7.069475664844911E-4 2.2442779888396545E-5 54-55 0.03600944033093226 8.079400759822756E-4 0.0 7.181689564286894E-4 2.2442779888396545E-5 56-57 0.04075608827732813 8.303828558706721E-4 0.0 7.181689564286894E-4 2.2442779888396545E-5 58-59 0.045008995066179266 8.640470257032669E-4 0.0 7.630545162054825E-4 2.2442779888396545E-5 60-61 0.050821675057273974 8.864898055916635E-4 0.0 7.85497296093879E-4 2.2442779888396545E-5 62-63 0.0578350437723979 0.0010211464849220427 0.0 8.079400759822756E-4 2.2442779888396545E-5 64-65 0.06702536213669627 0.001066032044698836 0.0 8.079400759822756E-4 2.2442779888396545E-5 66-67 0.07530674791551462 0.0010884748245872323 0.0 8.191614659264739E-4 2.2442779888396545E-5 68-69 0.08594462558261456 0.0011221389944198273 0.0 8.416042458148703E-4 2.2442779888396545E-5 70-71 0.0987370101190006 0.0011445817743082239 0.0 8.528256357590686E-4 2.2442779888396545E-5 72-73 0.11341458816601194 0.001155803164252422 0.0 8.640470257032669E-4 2.2442779888396545E-5 74-75 0.13011201640297895 0.0011782459441408186 0.0 8.977111955358617E-4 2.2442779888396545E-5 76-77 0.1488517376097901 0.0011894673340850169 0.0 8.977111955358617E-4 2.2442779888396545E-5 78-79 0.17041924908253914 0.0012231315039176118 0.0 9.0893258548006E-4 2.2442779888396545E-5 80-81 0.1985624950625884 0.001279238453638603 0.0 9.313753653684565E-4 2.2442779888396545E-5 82-83 0.23420162952536214 0.001324124013415396 0.0 9.87482315089448E-4 2.2442779888396545E-5 84-85 0.2771683316216973 0.0013353454033595944 0.0 0.0010099250949778444 2.2442779888396545E-5 86-87 0.32787779277952933 0.0013465667933037927 0.0 0.0010099250949778444 2.2442779888396545E-5 88 0.3707996093160877 0.0013465667933037927 0.0 0.001032367874866241 2.2442779888396545E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGAGT 1985 0.0 75.08175 1 CGTGTGG 1990 0.0 50.060947 3 AACGTGT 1705 0.0 49.634514 1 ATCGAGT 1015 0.0 45.856915 1 ACGTGTG 2065 0.0 45.739594 2 TCGAGTG 1250 0.0 45.111526 2 CGATTGG 580 0.0 43.750404 3 GTGGAAT 187065 0.0 43.556538 6 TGGAATC 188955 0.0 43.23975 7 ACGATTG 555 0.0 43.18108 2 AGTGGAA 186755 0.0 43.082268 5 GAATCAC 189780 0.0 43.079014 9 GGAATCA 190080 0.0 43.008556 8 ACGAGTG 197385 0.0 40.247883 2 AACGAGT 193435 0.0 40.222805 1 CGAGTGG 199975 0.0 40.027386 3 AGTACTT 41610 0.0 39.760975 20-21 GAGTACT 40960 0.0 39.54292 20-21 GAGTGGA 203625 0.0 39.540665 4 GTACTTT 40595 0.0 39.01461 22-23 >>END_MODULE