FastQCFastQC Report
Thu 26 May 2016
SRR2132708_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132708_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5534209
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2785225.032733675218988No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA376490.6802959555737776No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG316750.572349183053983No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64950.11736094534919082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAGT884850.069.2013851
ACGAGTG919100.068.0618062
GAATCAC951650.067.727739
TACGAGT19050.067.6335751
GTGGAAT953450.067.4717256
TGGAATC956550.067.4139567
GGAATCA958550.067.224288
CGAGTGG942000.066.921083
AGTGGAA958700.066.210635
GAGTGGA978300.064.927954
GAGTACT385850.040.94154420-21
AGTACTT398950.040.38052420-21
GTACTTT391600.039.68052722-23
ACGTGTG19650.038.9834752
TACTTTT418100.038.0085122-23
CATGGGG256350.037.9768524-25
AACGTGT18400.037.311421
ACTTTTT434100.035.5912524-25
TCGAGTG13750.034.520272
TGAGTAC951200.034.48352418-19
Produced by FastQC (version 0.11.5)