##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132708_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5534209
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.865764014333394	33.0	31.0	34.0	25.0	34.0
2	31.614345428587896	34.0	31.0	34.0	28.0	34.0
3	31.824840370141423	34.0	31.0	34.0	28.0	34.0
4	35.797114998728816	37.0	35.0	37.0	35.0	37.0
5	35.46875352918547	37.0	35.0	37.0	33.0	37.0
6	35.833811841945256	37.0	36.0	37.0	35.0	37.0
7	36.04534017417846	37.0	36.0	37.0	35.0	37.0
8	36.17010055095498	37.0	37.0	37.0	35.0	37.0
9	38.09904215760554	39.0	39.0	39.0	37.0	39.0
10-11	38.17541793235492	39.0	39.0	39.0	37.0	39.0
12-13	38.16760181988067	39.0	39.0	39.0	37.0	39.0
14-15	39.6974871205623	41.0	40.0	41.0	38.0	41.0
16-17	39.649535913804485	41.0	40.0	41.0	37.0	41.0
18-19	39.640220490407934	41.0	40.0	41.0	37.0	41.0
20-21	39.54904169683509	41.0	40.0	41.0	37.0	41.0
22-23	39.483211783291885	41.0	39.5	41.0	37.0	41.0
24-25	39.425495314687254	41.0	39.0	41.0	37.0	41.0
26-27	39.35909467820966	41.0	39.0	41.0	36.5	41.0
28-29	39.21908252832519	41.0	39.0	41.0	36.0	41.0
30-31	39.152554231327365	41.0	39.0	41.0	36.0	41.0
32-33	39.02992957078419	41.0	39.0	41.0	35.5	41.0
34-35	38.94124571370543	41.0	39.0	41.0	35.0	41.0
36-37	38.847247998765496	40.5	39.0	41.0	35.0	41.0
38-39	38.537178393515674	40.0	38.0	41.0	35.0	41.0
40-41	38.35731339383822	40.0	38.0	41.0	35.0	41.0
42-43	38.26692577024106	40.0	38.0	41.0	35.0	41.0
44-45	38.12384669606804	40.0	37.5	41.0	34.0	41.0
46-47	38.034197931447835	40.0	37.0	41.0	34.0	41.0
48-49	37.895937522417384	40.0	37.0	41.0	34.0	41.0
50-51	37.68586874836133	40.0	37.0	41.0	33.5	41.0
52-53	37.31966067779514	40.0	36.0	41.0	33.0	41.0
54-55	36.65520275797318	40.0	36.0	41.0	31.5	41.0
56-57	36.13972845983952	39.0	35.0	41.0	30.0	41.0
58-59	35.83897048340603	39.0	35.0	41.0	29.0	41.0
60-61	35.582984578283906	39.0	35.0	41.0	29.0	41.0
62-63	35.2861037593629	38.5	35.0	40.5	28.5	41.0
64-65	34.96950160357153	37.5	35.0	40.0	28.0	41.0
66-67	34.653859295881304	37.0	35.0	40.0	28.0	41.0
68-69	34.33673113899384	36.5	35.0	39.0	28.0	41.0
70-71	33.97933245744785	36.0	34.0	39.0	27.5	41.0
72-73	33.6258548421283	35.5	34.0	39.0	26.5	40.5
74-75	33.26180019583648	35.0	34.0	37.5	26.0	40.0
76-77	32.8839368914329	35.0	34.0	37.0	26.0	39.0
78-79	32.56826070356215	35.0	34.0	36.5	26.0	39.0
80-81	32.264356297349806	35.0	34.0	36.0	25.0	38.5
82-83	31.969067846913624	35.0	33.0	36.0	24.5	37.0
84-85	31.683645847130094	35.0	33.0	35.0	24.0	37.0
86-87	31.59702895210499	35.0	33.0	35.0	24.0	36.5
88-89	31.73893441682452	35.0	34.0	35.0	25.0	36.0
90-91	31.665542627681752	35.0	34.0	35.0	25.0	36.0
92-93	31.562450297775165	35.0	34.0	35.0	24.5	36.0
94-95	31.45037529301839	35.0	34.0	35.0	24.0	36.0
96-97	31.325891468862125	35.0	34.0	35.0	23.5	35.0
98-99	31.22626729492869	35.0	34.0	35.0	21.5	35.0
100	31.154989990439464	35.0	34.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.905518554909257
1101	2	1.4128839092159424
1101	3	1.2595775602891273
1101	4	0.6412691811790836
1101	5	0.9667098741524427
1101	6	0.6133758292665803
1101	7	0.40110537758728526
1101	8	0.3156962694051799
1101	9	0.29201426975868117
1101	10-11	0.17411970573384394
1101	12-13	0.22193422288861342
1101	14-15	0.2961112862655284
1101	16-17	0.30399486617632476
1101	18-19	0.25641612639185496
1101	20-21	0.3550741251494287
1101	22-23	0.3538722264452332
1101	24-25	0.33722564439386815
1101	26-27	0.3143983691010135
1101	28-29	0.3498754759341409
1101	30-31	0.3614801615916008
1101	32-33	0.32967630379137347
1101	34-35	0.3393929509091933
1101	36-37	0.35602929642636383
1101	38-39	0.3541467696508107
1101	40-41	0.3362153812558901
1101	42-43	0.32031771748944493
1101	44-45	0.3795995750968473
1101	46-47	0.37502184162152474
1101	48-49	0.29651724866790374
1101	50-51	0.22606884287297646
1101	52-53	0.25938832621955754
1101	54-55	0.25736865790685215
1101	56-57	0.2894577527061486
1101	58-59	0.21646868771194505
1101	60-61	0.1775743763592743
1101	62-63	0.16952951755328627
1101	64-65	0.17134281866327683
1101	66-67	0.17266541487304465
1101	68-69	0.1930049802072915
1101	70-71	0.12273397017503385
1101	72-73	0.15937944387299297
1101	74-75	0.11828423951293843
1101	76-77	0.15855283540116716
1101	78-79	0.1441970524077476
1101	80-81	0.19462430830216704
1101	82-83	0.16914641883511194
1101	84-85	0.15584291122177163
1101	86-87	0.23032633929410196
1101	88-89	0.16219822746036172
1101	90-91	0.21286695824194624
1101	92-93	0.22094562306591214
1101	94-95	0.1895263270113503
1101	96-97	0.2080358470073982
1101	98-99	0.197156720899212
1101	100	0.1798840932150796
1102	1	1.3979339483899196
1102	2	1.218647623226829
1102	3	1.1436666473404138
1102	4	0.5925741405470006
1102	5	0.9395943416805181
1102	6	0.5769770494740172
1102	7	0.3412798058441595
1102	8	0.30524957020657695
1102	9	0.2932596712817528
1102	10-11	0.24097075115598443
1102	12-13	0.20470678650765706
1102	14-15	0.2451751320845048
1102	16-17	0.28479290421599757
1102	18-19	0.3054802317798462
1102	20-21	0.3331630837935151
1102	22-23	0.3817750476213604
1102	24-25	0.3724767053689675
1102	26-27	0.30972509391325787
1102	28-29	0.395251820806223
1102	30-31	0.3827597086235528
1102	32-33	0.34579858537544794
1102	34-35	0.3123138640454357
1102	36-37	0.4115445128385886
1102	38-39	0.3950204132570505
1102	40-41	0.34475498984489406
1102	42-43	0.3424943403228511
1102	44-45	0.35892892929020803
1102	46-47	0.26602603254862345
1102	48-49	0.41669110587294966
1102	50-51	0.43861419990909667
1102	52-53	0.3499246996251557
1102	54-55	0.3166698472732463
1102	56-57	0.3642547353064387
1102	58-59	0.38118773921923577
1102	60-61	0.37628801590563654
1102	62-63	0.4052296223238159
1102	64-65	0.41410616705266534
1102	66-67	0.42910447447888345
1102	68-69	0.43420426638191145
1102	70-71	0.4490047485419808
1102	72-73	0.4843234272910877
1102	74-75	0.4362421613631682
1102	76-77	0.4092029763153562
1102	78-79	0.4714230977106979
1102	80-81	0.41058064053058274
1102	82-83	0.3556612683544813
1102	84-85	0.3946751663901864
1102	86-87	0.32072437301115997
1102	88-89	0.31783766088795673
1102	90-91	0.35435627835441963
1102	92-93	0.3957913357751437
1102	94-95	0.37095786176451284
1102	96-97	0.327451127019323
1102	98-99	0.3927281981001016
1102	100	0.39964025090717215
1103	1	1.8486925458170944
1103	2	1.3117811730395061
1103	3	1.2140249130007028
1103	4	0.6045375486353564
1103	5	0.9058724244184475
1103	6	0.5559080334050677
1103	7	0.35709433054551454
1103	8	0.23960025722982436
1103	9	0.26140967819528527
1103	10-11	0.17058659850187752
1103	12-13	0.16413897364137142
1103	14-15	0.23449811640742269
1103	16-17	0.23143469456669408
1103	18-19	0.2556163529305593
1103	20-21	0.29863515611919667
1103	22-23	0.3380369305782551
1103	24-25	0.2996559717160636
1103	26-27	0.2683438817322923
1103	28-29	0.31561423045234704
1103	30-31	0.27092918710344804
1103	32-33	0.26492854676506994
1103	34-35	0.21153579711872084
1103	36-37	0.2883656189335113
1103	38-39	0.32816205882659233
1103	40-41	0.290037893867499
1103	42-43	0.2727014032041666
1103	44-45	0.2683638239657995
1103	46-47	0.24824435467045447
1103	48-49	0.3088156106356408
1103	50-51	0.314279536986696
1103	52-53	0.25515603220906513
1103	54-55	0.18335544606473775
1103	56-57	0.12092531240762838
1103	58-59	0.11158301759690659
1103	60-61	0.14596337130151227
1103	62-63	0.054218365770168475
1103	64-65	0.03615213036609788
1103	66-67	0.06772999808337943
1103	68-69	0.09580653798887795
1103	70-71	0.05411581252946007
1103	72-73	0.034504357226310844
1103	74-75	0.06731193802403368
1103	76-77	0.042291576799371455
1103	78-79	0.08516305629564158
1103	80-81	0.10989096141150156
1103	82-83	0.12234081134621277
1103	84-85	0.11932229204514044
1103	86-87	0.03755688178431882
1103	88-89	0.046801248996843015
1103	90-91	0.060022760453541935
1103	92-93	0.07039797231161771
1103	94-95	0.0077339379245664475
1103	96-97	0.02949503177420354
1103	98-99	0.0479336436901896
1103	100	0.07633556998064961
1104	1	1.976422318713631
1104	2	1.4229198009672785
1104	3	1.2829653061920858
1104	4	0.7065746903559358
1104	5	1.0109714101844745
1104	6	0.6395157871654504
1104	7	0.3784638305470125
1104	8	0.31570638839530574
1104	9	0.27849851863064856
1104	10-11	0.25327343619154163
1104	12-13	0.23276905490571664
1104	14-15	0.3312953982244906
1104	16-17	0.3570550940989108
1104	18-19	0.333497916962493
1104	20-21	0.343359554263607
1104	22-23	0.39339194237759045
1104	24-25	0.4074400903255011
1104	26-27	0.3829134379638077
1104	28-29	0.37681836036760785
1104	30-31	0.4166947378435637
1104	32-33	0.3724264306620455
1104	34-35	0.38070066173299466
1104	36-37	0.39258690797673523
1104	38-39	0.4012483689112045
1104	40-41	0.3793215892563353
1104	42-43	0.3836837535590476
1104	44-45	0.37703042483137494
1104	46-47	0.4212923804884099
1104	48-49	0.35720527866917706
1104	50-51	0.3596274003956097
1104	52-53	0.4130732484643218
1104	54-55	0.2873356638663225
1104	56-57	0.3876114203034504
1104	58-59	0.33431460002322666
1104	60-61	0.3496500265427116
1104	62-63	0.34238777963206246
1104	64-65	0.3725299601865544
1104	66-67	0.2703437965705504
1104	68-69	0.25896017454556386
1104	70-71	0.25585281158132744
1104	72-73	0.2539319775301365
1104	74-75	0.265224548203463
1104	76-77	0.3250759197306934
1104	78-79	0.3142620899170083
1104	80-81	0.2570299602181052
1104	82-83	0.27047116329227805
1104	84-85	0.26885570254519564
1104	86-87	0.19936383935159796
1104	88-89	0.15871453513285516
1104	90-91	0.2023570837940838
1104	92-93	0.22818553052314527
1104	94-95	0.1979403439591252
1104	96-97	0.2236303606749317
1104	98-99	0.24055599631819646
1104	100	0.29996354261936276
1105	1	1.6037292432032757
1105	2	1.2795600290931048
1105	3	1.1675396498808475
1105	4	0.5807105797107823
1105	5	0.9436574584433259
1105	6	0.6131189676019986
1105	7	0.3149830395244635
1105	8	0.26615448462185043
1105	9	0.20416112437229827
1105	10-11	0.1861485536688221
1105	12-13	0.14732881309336676
1105	14-15	0.18753904823766732
1105	16-17	0.1793987115259128
1105	18-19	0.1863785574656447
1105	20-21	0.26750634602631607
1105	22-23	0.2580508067614815
1105	24-25	0.2785462562141561
1105	26-27	0.23358398850231765
1105	28-29	0.29551573970636014
1105	30-31	0.28458739681899203
1105	32-33	0.2640108531467362
1105	34-35	0.284657227056492
1105	36-37	0.264485665199075
1105	38-39	0.2755717699976401
1105	40-41	0.2673833073757521
1105	42-43	0.32995040918611807
1105	44-45	0.326899797683879
1105	46-47	0.32960378392285605
1105	48-49	0.2745802629127425
1105	50-51	0.2708921136025637
1105	52-53	0.30945981443824877
1105	54-55	0.3722946906646172
1105	56-57	0.38748171312227697
1105	58-59	0.3824052422065236
1105	60-61	0.3287977874980257
1105	62-63	0.35847042101353566
1105	64-65	0.23691908076169454
1105	66-67	0.2968104770851312
1105	68-69	0.34870384933327614
1105	70-71	0.35043538816166375
1105	72-73	0.29763354917155027
1105	74-75	0.2893348156893154
1105	76-77	0.36051240097894777
1105	78-79	0.28806660578957377
1105	80-81	0.28410176068419446
1105	82-83	0.2781602337211879
1105	84-85	0.29886042826692716
1105	86-87	0.2638356358473004
1105	88-89	0.2062714193134525
1105	90-91	0.2143648150619164
1105	92-93	0.25308318427009624
1105	94-95	0.2712285811890638
1105	96-97	0.3176039760408891
1105	98-99	0.23821247088782016
1105	100	0.2847255917168354
1106	1	1.5963079812196241
1106	2	1.2791340939513631
1106	3	1.1265303396870898
1106	4	0.6200613835784665
1106	5	0.9446876976725918
1106	6	0.6025310457662059
1106	7	0.375594230540635
1106	8	0.2732326687121187
1106	9	0.2687384641493793
1106	10-11	0.20647815039705364
1106	12-13	0.1500451416348625
1106	14-15	0.21353103908525384
1106	16-17	0.2424910872868722
1106	18-19	0.23035274474099765
1106	20-21	0.2698089993904702
1106	22-23	0.26190227651153464
1106	24-25	0.292893601379582
1106	26-27	0.2583029232105787
1106	28-29	0.32439810417996284
1106	30-31	0.37285523432849743
1106	32-33	0.3074739352689875
1106	34-35	0.25805261700357107
1106	36-37	0.26637441133608064
1106	38-39	0.23523528046216313
1106	40-41	0.27044580007295593
1106	42-43	0.2991560394506223
1106	44-45	0.30089885744903455
1106	46-47	0.28706497935647945
1106	48-49	0.29095838416237996
1106	50-51	0.2548306475091522
1106	52-53	0.29061829892844315
1106	54-55	0.3215993201803329
1106	56-57	0.3223818049320073
1106	58-59	0.3345527679592948
1106	60-61	0.33409713970625177
1106	62-63	0.28364645824558465
1106	64-65	0.26881265492682616
1106	66-67	0.24490905097358961
1106	68-69	0.3216804435374385
1106	70-71	0.2925864870172461
1106	72-73	0.31930956845067726
1106	74-75	0.2516918442000531
1106	76-77	0.28898238511308705
1106	78-79	0.21537359500422326
1106	80-81	0.2831930691398412
1106	82-83	0.330781424165437
1106	84-85	0.3175202642538153
1106	86-87	0.29266181332947383
1106	88-89	0.26020263916505826
1106	90-91	0.256040525869512
1106	92-93	0.24698585921178307
1106	94-95	0.2649918174063046
1106	96-97	0.21967237822656926
1106	98-99	0.21578113724672932
1106	100	0.24383906680691325
1107	1	1.6579990863363463
1107	2	1.3244157019099418
1107	3	1.2229880010640564
1107	4	0.6525086934974809
1107	5	0.9785152886902253
1107	6	0.6439825727797128
1107	7	0.3798992667158956
1107	8	0.2996526707950764
1107	9	0.29246418909214356
1107	10-11	0.1940765245602165
1107	12-13	0.11507159800896716
1107	14-15	0.2082022378027233
1107	16-17	0.19749623234294233
1107	18-19	0.1962495505099895
1107	20-21	0.25336793791945666
1107	22-23	0.2792130444006915
1107	24-25	0.24998516943662707
1107	26-27	0.22833106644878853
1107	28-29	0.2004545679426002
1107	30-31	0.1993334199615262
1107	32-33	0.225292096565056
1107	34-35	0.18318773673964728
1107	36-37	0.19620679036771804
1107	38-39	0.17872359413334493
1107	40-41	0.19960850421276888
1107	42-43	0.20697069550051594
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2209	9	0.18823911140137994
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2210	4	0.19023969602453406
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2210	10-11	0.03066311578750458
2210	12-13	-0.01738654854080579
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2212	76-77	-0.09321120284703
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2212	94-95	-0.06286095556155402
2212	96-97	-0.11771006388059391
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2213	1	0.13456756117284385
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2213	70-71	-0.1402567878494665
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2214	1	-3.605614025162147
2214	2	-3.907452303000799
2214	3	-3.4442853495329153
2214	4	-1.8661182564295586
2214	5	-2.816556009969318
2214	6	-1.467580822845349
2214	7	-0.9250345918512934
2214	8	-0.6949450692853247
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2214	16-17	-0.6161029097188973
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2214	30-31	-0.6335834573589807
2214	32-33	-0.6748160377032661
2214	34-35	-0.6511072445282693
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2214	80-81	-0.5741932179832361
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2214	84-85	-0.6659885695396284
2214	86-87	-0.6206484208514169
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2214	90-91	-0.5507677538547675
2214	92-93	-0.5748864796250643
2214	94-95	-0.6076003621096646
2214	96-97	-0.6177506999368063
2214	98-99	-0.6736500312121159
2214	100	-0.7098847648030748
2215	1	-3.69308246417598
2215	2	-3.5545823870582467
2215	3	-3.560776482575246
2215	4	-1.8242302870175564
2215	5	-2.757236988036823
2215	6	-1.3650419174664847
2215	7	-0.8893614259118365
2215	8	-0.6614861702886188
2215	9	-0.5928226036546107
2215	10-11	-0.5092918854531163
2215	12-13	-0.42845147384694116
2215	14-15	-0.6121993744074175
2215	16-17	-0.5810511460778898
2215	18-19	-0.6077127337631723
2215	20-21	-0.5774402713489621
2215	22-23	-0.6869156314965039
2215	24-25	-0.6301020418489145
2215	26-27	-0.6977199995175738
2215	28-29	-0.7941600094371779
2215	30-31	-0.812205331741211
2215	32-33	-0.7944055243952164
2215	34-35	-0.8851525242089195
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2215	40-41	-0.8672034268961042
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2215	54-55	-0.8533008960927759
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2215	68-69	-0.723290176160404
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2215	80-81	-0.7068657529538385
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2215	92-93	-0.6787011038975521
2215	94-95	-0.7023512509461973
2215	96-97	-0.71266372959413
2215	98-99	-0.7233448882529068
2215	100	-0.7613932754121961
2216	1	-6.191839154731767
2216	2	-6.1969630248598655
2216	3	-5.529281913421592
2216	4	-2.9863260694180624
2216	5	-4.000149986248221
2216	6	-4.27265764879915
2216	7	-2.006365766710921
2216	8	-1.299446569900013
2216	9	-1.0510994159685154
2216	10-11	-0.9074191299975425
2216	12-13	-0.765820110238792
2216	14-15	-1.0854999432285624
2216	16-17	-0.9823465857377016
2216	18-19	-0.971718764313394
2216	20-21	-1.049387507619251
2216	22-23	-1.0305484698824614
2216	24-25	-1.514191903304095
2216	26-27	-1.3160097971325229
2216	28-29	-1.9413616222041838
2216	30-31	-1.452308686221194
2216	32-33	-1.2478904086694484
2216	34-35	-1.219418147264662
2216	36-37	-1.2555162012895167
2216	38-39	-1.3217046524764413
2216	40-41	-1.3372676532987455
2216	42-43	-1.3591325515447323
2216	44-45	-1.2901072960306763
2216	46-47	-1.296846305163875
2216	48-49	-1.3265044230010048
2216	50-51	-1.2908388562385937
2216	52-53	-1.3088744774713703
2216	54-55	-1.331502883786726
2216	56-57	-1.270287801408962
2216	58-59	-1.256174143571961
2216	60-61	-1.2161637907312368
2216	62-63	-1.1992466743547254
2216	64-65	-1.2063110085770603
2216	66-67	-1.2964857368101264
2216	68-69	-1.3165753557595252
2216	70-71	-1.306070061769418
2216	72-73	-1.285810931383466
2216	74-75	-1.3318290048947112
2216	76-77	-1.2687133767952687
2216	78-79	-1.2881964199753781
2216	80-81	-1.2763471398652442
2216	82-83	-1.2900625623448718
2216	84-85	-1.2626119208187774
2216	86-87	-1.282672163063289
2216	88-89	-1.2237266345193305
2216	90-91	-1.1891879826281162
2216	92-93	-1.2331052353827978
2216	94-95	-1.2178713959505352
2216	96-97	-1.2992660034774168
2216	98-99	-1.332199431861774
2216	100	-1.3386464584213513
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4117.0
3	1968.0
4	2412.0
5	3091.0
6	2686.0
7	2261.0
8	2656.0
9	3129.0
10	3855.0
11	9056.0
12	6184.0
13	9121.0
14	8074.0
15	8026.0
16	9077.0
17	11619.0
18	15653.0
19	25815.0
20	57934.0
21	86982.0
22	40418.0
23	25682.0
24	27564.0
25	30617.0
26	31818.0
27	33161.0
28	37893.0
29	44737.0
30	54078.0
31	68563.0
32	91101.0
33	124907.0
34	188792.0
35	349674.0
36	629236.0
37	1290378.0
38	1676884.0
39	512551.0
40	2439.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.820622497253295	37.550769068333246	26.697013454469786	19.931594979943675
2	20.018094730086037	34.41907134679434	25.80815538555833	19.75467853756129
3	21.297822326551096	24.360211188265566	20.8732630083179	33.468703476865436
4	30.41369551574928	25.295802042249715	23.248510938635835	21.04199150336517
5	19.699564812442148	33.19516793989417	24.3433785604078	22.761888687255883
6	33.76435992361612	22.249949043683657	24.304247516150625	19.6814435165496
7	21.32543241500276	22.53803569760376	35.07502156134689	21.061510326046594
8	32.398722202215346	22.59999577175347	24.28589162425922	20.71539040177196
9	32.415111174876124	24.233652903242362	23.9028377858516	19.44839813602992
10-11	19.535989696088457	36.51144183387364	23.947704179585553	20.00486429045235
12-13	19.52547328805255	24.056933881608014	29.935922188699415	26.481670641640026
14-15	19.774789511149805	30.056464343855993	23.740791465723007	26.4279546792712
16-17	26.13216052621284	23.7538344614877	29.989041749857908	20.124963262441554
18-19	19.943961277935113	23.91615495547783	30.00838963617023	26.131494130416833
20-21	26.332697229179452	30.137414398335878	23.70936840296418	19.820519969520486
22-23	25.948455839916328	24.017176086325662	30.062803950557676	19.971564123200327
24-25	19.8562231647203	30.080874695721516	23.705767246181917	26.357134893376266
26-27	20.133517184649772	26.75597749438701	33.10697069176181	20.003534629201404
28-29	26.406215159484407	23.72094086422729	29.98807765461916	19.884766321669144
30-31	24.917470204839777	24.70129395840428	30.13090833326632	20.250327503489622
32-33	21.897330243730423	25.567602501208654	30.951449703738376	21.583617551322547
34-35	21.73153233101596	25.412061968119477	31.240302231492116	21.616103469372447
36-37	21.68623296797361	25.24527310886399	31.39555437632799	21.672939546834414
38-39	22.40851718937739	25.24011594533985	30.902464001133573	21.44890286414919
40-41	21.445515807254438	26.08081787139418	30.965637786129673	21.508028535221708
42-43	21.650490147491944	25.357065380074488	31.32307070128086	21.66937377115271
44-45	21.5363852128026	25.538507438088814	30.98605229593911	21.93905505316948
46-47	21.910866869498633	25.286704567784952	31.309278094373333	21.49315046834308
48-49	21.64844833839413	25.346354247139306	31.176842745548967	21.828354668917598
50-51	22.043557212404185	25.638471577017363	30.66249318150748	21.65547802907097
52-53	22.12604578883649	25.543620462225114	30.723920782060315	21.606412966878082
54-55	22.08282857689904	26.1433247141852	29.553820160240797	22.220026548674966
56-57	22.45596848992731	26.55905260885754	28.963798166177092	22.021180735038058
58-59	23.188201248434893	26.692269193017125	28.187226741658662	21.93230281688932
60-61	22.81409542382842	27.245809205832668	27.943314137348036	21.99678123299088
62-63	22.235666022394227	27.063932768711794	28.0531633632232	22.647237845670777
64-65	22.413917736486546	27.05355177549921	28.419191807840484	22.113338680173765
66-67	22.44158200339451	26.57418571693818	28.872298315890195	22.111933963777116
68-69	22.719759772045094	26.433125162515807	28.84191889632183	22.005196169117273
70-71	21.94803947443677	27.123391814080794	29.021387543384847	21.907181168097594
72-73	21.868219946220815	26.37123503017885	29.333454163775784	22.42709085982455
74-75	22.040125722903987	26.706973219098707	28.729143690263232	22.523757367734074
76-77	22.549334340477763	26.41420290496716	28.922317811593214	22.114144942961865
78-79	22.27251180423165	26.72385516528093	28.53851802066615	22.465115009821275
80-81	22.684412193066493	27.15634231135966	28.077335962773176	22.08190953280067
82-83	22.58794138758834	27.033361091172214	28.3459671257553	22.032730395484144
84-85	22.41660262865316	27.465011752726078	27.629003279378768	22.489382339242
86-87	22.497461833530256	27.39729777888296	27.874850773160386	22.2303896144264
88-89	23.163040473640258	27.171891137128263	27.500411384521044	22.16465700471043
90-91	22.840458775832705	27.66888049638847	27.371243322410876	22.11941740536795
92-93	22.40268368681995	27.599977481724913	27.327517196615712	22.669821634839423
94-95	22.63687725660636	27.87030209578596	27.222005928740366	22.270814718867314
96-97	22.67643858103881	27.40684231583962	27.601757744662596	22.31496135845898
98-99	23.100346275900772	27.451408105228982	27.10427376624583	22.343971852624414
100	22.421634879777407	28.235244365946844	27.017702260886285	22.32541849338946
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	37905.0
1	20654.0
2	2613.0
3	1638.5
4	1485.0
5	1416.0
6	1394.5
7	1652.0
8	1616.0
9	1514.5
10	1570.0
11	2365.5
12	27747.0
13	32615.5
14	12220.0
15	12040.5
16	13459.0
17	15125.5
18	16785.5
19	18330.5
20	19951.5
21	21888.5
22	23935.5
23	26634.5
24	30055.5
25	33823.5
26	38413.0
27	43714.5
28	48767.0
29	54630.0
30	61845.0
31	69295.0
32	78510.0
33	89290.5
34	100875.5
35	109736.5
36	117118.5
37	124355.0
38	128578.0
39	134295.0
40	141251.0
41	146016.5
42	149949.5
43	156584.0
44	166915.0
45	174820.0
46	177622.5
47	183596.5
48	189102.5
49	197930.0
50	233561.0
51	234423.0
52	196780.5
53	183528.0
54	179314.0
55	174712.0
56	166762.5
57	155787.0
58	144791.5
59	128233.5
60	107591.5
61	88923.0
62	72615.0
63	57338.5
64	43396.0
65	32327.5
66	23670.5
67	17614.5
68	13230.0
69	9675.0
70	7143.0
71	5336.5
72	3846.5
73	2757.5
74	2026.5
75	1422.5
76	964.5
77	632.5
78	416.5
79	275.0
80	162.5
81	96.5
82	61.5
83	32.0
84	16.5
85	12.0
86	11.0
87	9.0
88	6.0
89	3.0
90	3.5
91	2.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05485878831103054
2	1.8069429614963944E-5
3	0.0
4	3.25249733069351E-4
5	0.009088923096326864
6	0.0010299574880529448
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.5359015172719352E-4
16-17	4.0656216633668874E-4
18-19	0.0
20-21	0.0
22-23	5.420828884489184E-5
24-25	0.0039029967968322123
26-27	0.0070380428350284565
28-29	0.0011383740657427285
30-31	0.005393724740066738
32-33	0.002972421171661569
34-35	0.02464670199481082
36-37	0.02524299317210463
38-39	0.02271327302600968
40-41	0.0024574424276350964
42-43	0.005664766184291197
44-45	0.0071374246979107586
46-47	0.001291964217469922
48-49	0.008962437089022118
50-51	0.009838804425347868
52-53	0.0019695678280310697
54-55	0.010832623054170886
56-57	0.006495959946579539
58-59	0.011573469668384406
60-61	0.007462674430980109
62-63	0.0010751310620903548
64-65	0.013064197611618932
66-67	0.004996197288537531
68-69	0.0023851647091752406
70-71	0.008140278041541257
72-73	0.005122683295842278
74-75	0.008167382185963704
76-77	0.007435570286557663
78-79	0.014672376847350723
80-81	0.008266764048846005
82-83	0.011510226664732033
84-85	0.006983834546183564
86-87	0.012413698145480231
88-89	0.01923490782512912
90-91	0.017382791289595315
92-93	0.016723257108649133
94-95	0.005755113332366017
96-97	0.009522589407085998
98-99	0.01634379908673489
100	0.015322876313489425
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5534209.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.75952922712117
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.77637750547497	51.32246954142949
2	12.11732006112488	15.209546050610973
3	2.951985918636397	5.557957396161332
4	1.0578758628236131	2.655671645261783
5	0.5347623075094057	1.6780715333849654
6	0.32543071864484113	1.2254327218916374
7	0.21781243171552644	0.956886397199664
8	0.15338934470823745	0.7701314449876474
9	0.11200333248035724	0.6326348776502354
>10	0.7091576787063268	8.959199030469334
>50	0.033533069124127915	1.3661820647110339
>100	0.007356673538349667	0.8932857327146673
>500	0.0013535550571706413	0.6173942482588513
>1k	0.001497545679042679	1.6550262382182248
>5k	5.759791072914038E-5	0.21311158114848727
>10k+	8.639686609371056E-5	6.2869994959016795
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	278522	5.032733675218988	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	37649	0.6802959555737776	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	31675	0.572349183053983	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6495	0.11736094534919082	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0018611512503412865	0.0	0.0	0.0	7.227771845985578E-5
2	0.0019153595391861783	1.8069429614963944E-5	0.0	0.0	9.034714807481972E-5
3	0.0019153595391861783	3.613885922992789E-5	0.0	0.0	1.264860073047476E-4
4	0.0019153595391861783	3.613885922992789E-5	0.0	0.0	1.4455543691971155E-4
5	0.0019153595391861783	5.420828884489183E-5	0.0	0.0	1.626248665346755E-4
6	0.0019334289688011422	5.420828884489183E-5	0.0	0.0	1.8069429614963944E-4
7	0.0019334289688011422	7.227771845985578E-5	0.0	0.0	1.9876372576460338E-4
8	0.0019334289688011422	1.264860073047476E-4	0.0	0.0	2.1683315537956733E-4
9	0.0019334289688011422	1.264860073047476E-4	0.0	0.0	2.1683315537956733E-4
10-11	0.0019334289688011422	1.264860073047476E-4	0.0	0.0	2.620067294169772E-4
12-13	0.0019424636836086243	1.4455543691971155E-4	0.0	0.0	2.981455886469051E-4
14-15	0.0019876372576460343	1.7165958134215747E-4	0.0	0.0	3.25249733069351E-4
16-17	0.002041845546490926	1.8069429614963944E-4	0.0	0.0	3.25249733069351E-4
18-19	0.0020960538353358175	2.258678701870493E-4	0.0	0.0	3.9752745152920677E-4
20-21	0.0021141232649507817	2.349025849945313E-4	0.0	0.0	4.5173574037409866E-4
22-23	0.002159296838988191	2.349025849945313E-4	0.0	0.0	5.601523180638823E-4
24-25	0.0022767481314854574	2.4393729980201324E-4	0.0	0.0	5.601523180638823E-4
26-27	0.0023851647091752406	2.529720146094952E-4	0.0	0.0	5.782217476788462E-4
28-29	0.0025568242905173984	2.529720146094952E-4	0.0	0.0	5.782217476788462E-4
30-31	0.0026742755830146637	2.7104144422445916E-4	0.0	0.0	6.053258921012921E-4
32-33	0.002809796305126893	2.981455886469051E-4	0.0	0.0	6.776036105611479E-4
34-35	0.002909178168009195	3.16215018261869E-4	0.0	0.0	7.227771845985578E-4
36-37	0.003071803034543871	3.25249733069351E-4	0.0	0.0	7.860201882509316E-4
38-39	0.003261532045500992	3.523538774917969E-4	0.0	0.0	8.040896178658955E-4
40-41	0.0035777470637628614	3.794580219142428E-4	0.0	0.0	8.221590474808595E-4
42-43	0.003921066226447176	3.9752745152920677E-4	0.0	0.0	8.582979067107873E-4
44-45	0.004381836681628757	3.9752745152920677E-4	0.0	0.0	8.944367659407153E-4
46-47	0.00485164185161782	4.246315959516527E-4	0.0	1.8069429614963944E-5	9.576797695930891E-4
48-49	0.0053575858808368095	4.4270102556661663E-4	0.0	1.8069429614963944E-5	9.93818628823017E-4
50-51	0.005872564624863282	4.6077045518158063E-4	0.0	1.8069429614963944E-5	0.001020922773245463
52-53	0.006423682228119683	4.8787459960402654E-4	0.0	1.8069429614963944E-5	0.0010751310620903546
54-55	0.007309084279252916	4.969093144115085E-4	0.0	1.8069429614963944E-5	0.0011293393509352466
56-57	0.008284833478460969	5.059440292189904E-4	0.0	1.8069429614963944E-5	0.0012016170693951024
58-59	0.009043749522289455	5.330481736414363E-4	0.0	1.8069429614963944E-5	0.0012106517842025843
60-61	0.009974325147460097	5.872564624863282E-4	0.0	1.8069429614963944E-5	0.0012196864990100661
62-63	0.011582504383191888	6.233953217162561E-4	0.0	1.8069429614963944E-5	0.0012377559286250303
64-65	0.013823113655447418	6.50499466138702E-4	0.0	1.8069429614963944E-5	0.0013281030766998498
66-67	0.016235382509045104	6.685688957536659E-4	0.0	1.8069429614963944E-5	0.0013552072211222958
68-69	0.01915359539186178	6.776036105611479E-4	0.0	1.8069429614963944E-5	0.0013823113655447418
70-71	0.022704238311202198	6.956730401761118E-4	0.0	1.8069429614963944E-5	0.0014636237988120795
72-73	0.027402290011092824	7.408466142135217E-4	0.0	1.8069429614963944E-5	0.0014907279432345255
74-75	0.03328388935076359	7.860201882509316E-4	0.0	1.8069429614963944E-5	0.002032810831683444
76-77	0.04101760522596816	7.950549030584135E-4	0.0	1.8069429614963944E-5	0.0024393729980201327
78-79	0.05007038946306509	8.040896178658955E-4	0.0	1.8069429614963944E-5	0.0024755118572500606
80-81	0.06186972700163655	8.492631919033054E-4	0.0	1.8069429614963944E-5	0.0025116507164799884
82-83	0.07649693027494986	8.854020511332333E-4	0.0	1.8069429614963944E-5	0.002574893720132362
84-85	0.09513554692278517	9.215409103631613E-4	0.0	1.8069429614963944E-5	0.00261103257936229
86-87	0.1184270416964737	9.396103399781252E-4	0.0	1.8069429614963944E-5	0.002638136723784736
88	0.13889970545022784	9.396103399781252E-4	0.0	1.8069429614963944E-5	0.002692345012629628
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGAGT	88485	0.0	69.201385	1
ACGAGTG	91910	0.0	68.061806	2
GAATCAC	95165	0.0	67.72773	9
TACGAGT	1905	0.0	67.633575	1
GTGGAAT	95345	0.0	67.471725	6
TGGAATC	95655	0.0	67.413956	7
GGAATCA	95855	0.0	67.22428	8
CGAGTGG	94200	0.0	66.92108	3
AGTGGAA	95870	0.0	66.21063	5
GAGTGGA	97830	0.0	64.92795	4
GAGTACT	38585	0.0	40.941544	20-21
AGTACTT	39895	0.0	40.380524	20-21
GTACTTT	39160	0.0	39.680527	22-23
ACGTGTG	1965	0.0	38.983475	2
TACTTTT	41810	0.0	38.00851	22-23
CATGGGG	25635	0.0	37.97685	24-25
AACGTGT	1840	0.0	37.31142	1
ACTTTTT	43410	0.0	35.59125	24-25
TCGAGTG	1375	0.0	34.52027	2
TGAGTAC	95120	0.0	34.483524	18-19
>>END_MODULE