FastQCFastQC Report
Thu 26 May 2016
SRR2132707_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132707_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4479732
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3534217.889333558346793No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3261957.281573987015294No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG585861.3078014488366714No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT92640.20679808524259932No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79760.1780463652736369No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70820.15808981430139124No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT67470.15061168837778688No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT66100.14755346971649197No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT62540.1396065657499154No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC49980.11156917422738682No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47820.10674745721395834No Hit
TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG46650.10413569383168457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT22000.076.078881
GTGGAAT1300100.062.160716
AACGAGT1243500.061.9456631
AGTGGAA1296250.061.5265855
TGGAATC1317600.061.492027
GAATCAC1327350.061.0792739
ACGAGTG1293550.060.9909022
GGAATCA1329450.060.9792568
CGAGTGG1319700.060.138443
GAGTGGA1348950.059.20024
TCGAGTG10550.055.232242
AACGTGT15250.055.1848641
ACGTGTG17850.052.6520422
CGTGTGG20450.050.0941053
ATCGAGT12400.041.7068861
AGTACTT525650.040.61924420-21
GAGTACT520050.040.0716820-21
GTACTTT521650.039.26630822-23
TACTTTT543550.038.613722-23
CATGGGG239800.037.75713724-25
Produced by FastQC (version 0.11.5)