##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132707_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4479732
Sequences flagged as poor quality	0
Sequence length	100
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.87200328055339	33.0	31.0	34.0	25.0	34.0
2	31.610474912338507	34.0	31.0	34.0	28.0	34.0
3	31.80059610708855	34.0	31.0	34.0	28.0	34.0
4	35.71391368947964	37.0	35.0	37.0	33.0	37.0
5	35.34358350008438	37.0	35.0	37.0	33.0	37.0
6	35.642293333619065	37.0	35.0	37.0	35.0	37.0
7	35.81210728677519	37.0	35.0	37.0	35.0	37.0
8	35.91805938390957	37.0	37.0	37.0	35.0	37.0
9	37.78545814794278	39.0	39.0	39.0	35.0	39.0
10-11	37.812474384628366	39.0	39.0	39.0	37.0	39.0
12-13	37.72112249125617	39.0	39.0	39.0	37.0	39.0
14-15	39.19335252644578	41.0	40.0	41.0	37.0	41.0
16-17	39.02068103627627	41.0	39.5	41.0	37.0	41.0
18-19	38.6778636534507	41.0	39.0	41.0	35.5	41.0
20-21	38.45418118762461	41.0	39.0	41.0	35.0	41.0
22-23	38.313890652387244	40.5	38.0	41.0	35.0	41.0
24-25	38.214055550644545	41.0	38.0	41.0	35.0	41.0
26-27	38.11107584114407	40.5	38.0	41.0	35.0	41.0
28-29	37.9350395291504	41.0	38.0	41.0	34.5	41.0
30-31	37.8122508221474	41.0	38.0	41.0	34.0	41.0
32-33	37.65882177773135	40.0	38.0	41.0	34.0	41.0
34-35	37.551845847028346	40.0	37.0	41.0	34.0	41.0
36-37	37.38593938208804	40.0	37.0	41.0	33.0	41.0
38-39	36.925649570108206	40.0	36.0	41.0	33.0	41.0
40-41	36.716072077526064	40.0	35.0	41.0	33.0	41.0
42-43	36.603846948879976	40.0	35.0	41.0	33.0	41.0
44-45	36.43147090049137	40.0	35.0	41.0	33.0	41.0
46-47	36.32117267282953	39.0	35.0	41.0	32.5	41.0
48-49	36.152571961894154	39.0	35.0	41.0	31.5	41.0
50-51	35.89381608096198	39.0	35.0	41.0	31.0	41.0
52-53	35.40897647448553	39.0	35.0	41.0	29.0	41.0
54-55	34.5594208537475	38.0	35.0	41.0	22.5	41.0
56-57	33.94908233349673	38.0	35.0	41.0	14.0	41.0
58-59	33.665230643261694	38.0	35.0	41.0	7.5	41.0
60-61	33.42847752945936	37.0	35.0	40.0	7.0	41.0
62-63	33.1786727420301	37.0	34.5	40.0	3.5	41.0
64-65	32.88148241903757	36.0	34.0	40.0	2.0	41.0
66-67	32.56175045739343	35.5	34.0	39.0	2.0	41.0
68-69	32.294170945940515	35.0	34.0	39.0	2.0	41.0
70-71	32.01352011682842	35.0	34.0	38.5	2.0	41.0
72-73	31.71965610889223	35.0	33.0	37.5	2.0	40.0
74-75	31.415736588706647	35.0	33.0	37.0	2.0	39.5
76-77	31.083183770814863	35.0	33.0	36.5	2.0	39.0
78-79	30.801348607461343	35.0	33.0	36.0	2.0	39.0
80-81	30.52666744349885	35.0	33.0	36.0	2.0	38.5
82-83	30.228649727260468	35.0	32.5	35.0	2.0	37.0
84-85	29.94735678384332	35.0	32.0	35.0	2.0	37.0
86-87	29.862992585270725	35.0	32.0	35.0	2.0	36.0
88-89	29.927919906815852	35.0	33.0	35.0	2.0	36.0
90-91	29.811055103296358	35.0	33.0	35.0	2.0	36.0
92-93	29.685522482148485	35.0	33.0	35.0	2.0	36.0
94-95	29.52967822628675	35.0	33.0	35.0	2.0	35.5
96-97	29.343147648118237	35.0	32.5	35.0	2.0	35.0
98-99	29.222275685241883	35.0	32.0	35.0	2.0	35.0
100	29.15860524692102	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.8690509810834008
1101	2	1.3788160714506432
1101	3	1.2507284298651058
1101	4	0.6844947568220121
1101	5	0.9482862120201929
1101	6	0.5316444120607855
1101	7	0.32227991665217104
1101	8	0.21291119335539577
1101	9	0.27071741512072833
1101	10-11	0.22243359576979316
1101	12-13	0.33690324253971937
1101	14-15	0.45812458944068624
1101	16-17	0.498860933284881
1101	18-19	0.4610188110921385
1101	20-21	0.5647203641199852
1101	22-23	0.5841346453859586
1101	24-25	0.527642572885938
1101	26-27	0.582547255657488
1101	28-29	0.6205219007241567
1101	30-31	0.6265173702650841
1101	32-33	0.5600722827370248
1101	34-35	0.5496388032181088
1101	36-37	0.5336951910531482
1101	38-39	0.525968391718699
1101	40-41	0.5127394989435032
1101	42-43	0.5756854780573093
1101	44-45	0.584039198224886
1101	46-47	0.5188619291375787
1101	48-49	0.47365492603435655
1101	50-51	0.5149610850892188
1101	52-53	0.5278496920828744
1101	54-55	0.40011550538935126
1101	56-57	0.3119552334149063
1101	58-59	0.35281476337249984
1101	60-61	0.35060302769308294
1101	62-63	0.38929844342404607
1101	64-65	0.3987149005046078
1101	66-67	0.41450570575170076
1101	68-69	0.48404929982798706
1101	70-71	0.47747595784386476
1101	72-73	0.5053433433898853
1101	74-75	0.5020547634992099
1101	76-77	0.5249799926121241
1101	78-79	0.5452801626288881
1101	80-81	0.5140353732390182
1101	82-83	0.49940939008375906
1101	84-85	0.5333952265401649
1101	86-87	0.5652239659884621
1101	88-89	0.5470031719242243
1101	90-91	0.5051990109849953
1101	92-93	0.4714719675490677
1101	94-95	0.44309874770666724
1101	96-97	0.4642475508779462
1101	98-99	0.4761116485987067
1101	100	0.5018954463569294
1102	1	1.2214766914926862
1102	2	1.126966016146838
1102	3	1.0755849204472838
1102	4	0.5239420184329049
1102	5	0.7814563954702223
1102	6	0.4315057887547695
1102	7	0.2569394432394603
1102	8	0.19434872883143584
1102	9	0.24657074553942948
1102	10-11	0.2632927421897264
1102	12-13	0.32408191205567505
1102	14-15	0.45634255833390824
1102	16-17	0.47916589968958334
1102	18-19	0.5471365519704179
1102	20-21	0.6142409382344738
1102	22-23	0.650107540975597
1102	24-25	0.6397385247078233
1102	26-27	0.6158255989331707
1102	28-29	0.6804272543177206
1102	30-31	0.7357683013310705
1102	32-33	0.7403137526785315
1102	34-35	0.6468336016363168
1102	36-37	0.7173331781233045
1102	38-39	0.7097486616073425
1102	40-41	0.6428687735881056
1102	42-43	0.6496157982351676
1102	44-45	0.6610417237482977
1102	46-47	0.6018441737673044
1102	48-49	0.7069914861218507
1102	50-51	0.6249460824404736
1102	52-53	0.6421370508501738
1102	54-55	0.5287262987386541
1102	56-57	0.4777749514955829
1102	58-59	0.5416584948332002
1102	60-61	0.5610966161352735
1102	62-63	0.5283261350333319
1102	64-65	0.5164743603443398
1102	66-67	0.4901404039668833
1102	68-69	0.5072125937657077
1102	70-71	0.525839539089823
1102	72-73	0.5876579636588097
1102	74-75	0.5454404476054364
1102	76-77	0.532597458499545
1102	78-79	0.5618545760425739
1102	80-81	0.518871907171679
1102	82-83	0.45580873328647087
1102	84-85	0.47630575013499055
1102	86-87	0.46803026298973194
1102	88-89	0.4634829816663597
1102	90-91	0.4670325273480458
1102	92-93	0.4770676136542491
1102	94-95	0.47290876302144724
1102	96-97	0.4501079033411983
1102	98-99	0.5087121300747839
1102	100	0.5754469677755729
1103	1	1.8526953672710995
1103	2	1.3293371699758367
1103	3	1.2429454806450693
1103	4	0.6378907718441269
1103	5	0.8619587049393544
1103	6	0.46521068151948697
1103	7	0.28725545055423396
1103	8	0.1769579595415678
1103	9	0.2183634378679713
1103	10-11	0.16573246741232595
1103	12-13	0.26842452933510685
1103	14-15	0.39623066041610144
1103	16-17	0.4462215509639975
1103	18-19	0.4637231641472539
1103	20-21	0.5255257346942201
1103	22-23	0.5513513961866323
1103	24-25	0.5498714890411449
1103	26-27	0.5720048952378463
1103	28-29	0.6331726642059436
1103	30-31	0.578227793732836
1103	32-33	0.5362028781072397
1103	34-35	0.5224671310741229
1103	36-37	0.5695415738392455
1103	38-39	0.615942346083834
1103	40-41	0.573992135973775
1103	42-43	0.6081577310355186
1103	44-45	0.5852223908475906
1103	46-47	0.5735750178017938
1103	48-49	0.6381861561076363
1103	50-51	0.6535186222306422
1103	52-53	0.6889978726102939
1103	54-55	0.6348573644559963
1103	56-57	0.6352154305602227
1103	58-59	0.5939472695841914
1103	60-61	0.5795288194021282
1103	62-63	0.5340341468096312
1103	64-65	0.5056015806492837
1103	66-67	0.6182739870997764
1103	68-69	0.6271828749216155
1103	70-71	0.6091744819564084
1103	72-73	0.6155265569314956
1103	74-75	0.6144975112584632
1103	76-77	0.6232009083995003
1103	78-79	0.623554924962999
1103	80-81	0.6461202865959734
1103	82-83	0.6640141549015937
1103	84-85	0.5934658390086653
1103	86-87	0.6313008804642095
1103	88-89	0.6357555734840332
1103	90-91	0.6081219560233748
1103	92-93	0.5882763609872406
1103	94-95	0.6079327975745876
1103	96-97	0.634625111705514
1103	98-99	0.6456382239492022
1103	100	0.6590592648840108
1104	1	1.8359470979042136
1104	2	1.3522184391039005
1104	3	1.256881183112931
1104	4	0.6248964372160231
1104	5	0.8147625697122365
1104	6	0.348735791852107
1104	7	0.0911859286903578
1104	8	-0.01832880391081204
1104	9	0.013276200899731805
1104	10-11	1.6263706481822737E-4
1104	12-13	0.0878508149921089
1104	14-15	0.1990229860220012
1104	16-17	0.23192320133227895
1104	18-19	0.2772771572873225
1104	20-21	0.29391193673339444
1104	22-23	0.33479547258544073
1104	24-25	0.3478267236947232
1104	26-27	0.3415576456827196
1104	28-29	0.35848816784971405
1104	30-31	0.3480996730706849
1104	32-33	0.31518333935099463
1104	34-35	0.28856775800921497
1104	36-37	0.3117372921690702
1104	38-39	0.2958028146091607
1104	40-41	0.30615840561601715
1104	42-43	0.34878532215915925
1104	44-45	0.37792580196389025
1104	46-47	0.37018093902689486
1104	48-49	0.3500006433827991
1104	50-51	0.31207110593255294
1104	52-53	0.3526099410458343
1104	54-55	0.36240892232504507
1104	56-57	0.319454950559404
1104	58-59	0.27034484199405995
1104	60-61	0.2548886944663806
1104	62-63	0.24238836108607842
1104	64-65	0.20629325069501903
1104	66-67	0.16154735309795853
1104	68-69	0.23092227810296606
1104	70-71	0.23143829741570698
1104	72-73	0.25170235220574355
1104	74-75	0.22465706908349858
1104	76-77	0.32541644373968026
1104	78-79	0.3127016597200054
1104	80-81	0.2610039376159108
1104	82-83	0.28419168846182785
1104	84-85	0.32199502994319573
1104	86-87	0.3468174895555869
1104	88-89	0.30143259507809006
1104	90-91	0.328386533765503
1104	92-93	0.33390896073583676
1104	94-95	0.3057609027476147
1104	96-97	0.3469026427546851
1104	98-99	0.35730391938355055
1104	100	0.3356521448894618
1105	1	1.551972663578109
1105	2	1.2436732641949888
1105	3	1.1736258064926446
1105	4	0.611281483637903
1105	5	0.9353867447589224
1105	6	0.5901173802695254
1105	7	0.24658496094249216
1105	8	0.16696734422869497
1105	9	0.11831489638281312
1105	10-11	0.16165365309893076
1105	12-13	0.2154748125439383
1105	14-15	0.3235977476836638
1105	16-17	0.33967379379384255
1105	18-19	0.3699776780663768
1105	20-21	0.4570722062048773
1105	22-23	0.48816720269901737
1105	24-25	0.5282266902926409
1105	26-27	0.5094121176589468
1105	28-29	0.5814835459526151
1105	30-31	0.5739230043815624
1105	32-33	0.5685146243188086
1105	34-35	0.5010143784959737
1105	36-37	0.5100253856929484
1105	38-39	0.5297651548622397
1105	40-41	0.48185509144530414
1105	42-43	0.5080317991205305
1105	44-45	0.5444038685206323
1105	46-47	0.5160933952066955
1105	48-49	0.4838916896613483
1105	50-51	0.4990139598648895
1105	52-53	0.44896416700850494
1105	54-55	0.45031972147499033
1105	56-57	0.40424132211177266
1105	58-59	0.3192449844216583
1105	60-61	0.3509023251835828
1105	62-63	0.4485672667778431
1105	64-65	0.39023551639039056
1105	66-67	0.4172062876576135
1105	68-69	0.462094186994193
1105	70-71	0.4616862215954356
1105	72-73	0.4229562639692155
1105	74-75	0.42986365077547717
1105	76-77	0.4743448693997401
1105	78-79	0.45066380163276065
1105	80-81	0.46803381953557377
1105	82-83	0.4166961409432197
1105	84-85	0.3980047061883738
1105	86-87	0.41676536477929105
1105	88-89	0.3676303208989786
1105	90-91	0.333159591353148
1105	92-93	0.36916584168365674
1105	94-95	0.36031929516469674
1105	96-97	0.3628828787821483
1105	98-99	0.300750307116445
1105	100	0.3128980685207843
1106	1	1.5994798122394691
1106	2	1.299565490573606
1106	3	1.1568372907845443
1106	4	0.6965075045283342
1106	5	1.0131903585345796
1106	6	0.7271707814213642
1106	7	0.45872951196864875
1106	8	0.24862933988382707
1106	9	0.1516007693960546
1106	10-11	0.05902920005740242
1106	12-13	0.07812480413375056
1106	14-15	0.15886007453443085
1106	16-17	0.26319525873045535
1106	18-19	0.29046401210015915
1106	20-21	0.3225264038372373
1106	22-23	0.36531422940078073
1106	24-25	0.36372539085908073
1106	26-27	0.36066345914345277
1106	28-29	0.43846696484877157
1106	30-31	0.43707968029548994
1106	32-33	0.3814397831055629
1106	34-35	0.43574443509299243
1106	36-37	0.4812807782829793
1106	38-39	0.47502038635568056
1106	40-41	0.42401886745470563
1106	42-43	0.46696424989723084
1106	44-45	0.46845221408230486
1106	46-47	0.5450810689481926
1106	48-49	0.5167667071332147
1106	50-51	0.5088332710821888
1106	52-53	0.5793755911260945
1106	54-55	0.5810518581133266
1106	56-57	0.5539282498338878
1106	58-59	0.5972085102575306
1106	60-61	0.5559534141254829
1106	62-63	0.5048787588876635
1106	64-65	0.4360263348321638
1106	66-67	0.381472930949478
1106	68-69	0.4745996062362394
1106	70-71	0.4547666490699811
1106	72-73	0.4740388093901551
1106	74-75	0.4608739159452071
1106	76-77	0.5027041479712828
1106	78-79	0.49136266471723644
1106	80-81	0.5739855687366031
1106	82-83	0.614548344294505
1106	84-85	0.5917323036932736
1106	86-87	0.578524646193717
1106	88-89	0.5544568246053849
1106	90-91	0.5293808693703355
1106	92-93	0.6142971709437752
1106	94-95	0.5778036060147933
1106	96-97	0.5271156444079246
1106	98-99	0.5466473190115302
1106	100	0.6264045964864451
1107	1	1.5960779124301787
1107	2	1.2278284379506132
1107	3	1.1638320225961536
1107	4	0.6700515049994422
1107	5	1.0026311061371658
1107	6	0.7096398993282236
1107	7	0.48255911800962537
1107	8	0.39394119579910125
1107	9	0.34303260229980737
1107	10-11	0.11598520185121686
1107	12-13	0.01589246026554747
1107	14-15	0.13334367429706617
1107	16-17	0.1825248217380775
1107	18-19	0.14477199048383227
1107	20-21	0.21132017894946387
1107	22-23	0.2519052630619214
1107	24-25	0.24707601303077098
1107	26-27	0.2899648692784851
1107	28-29	0.2548074084999996
1107	30-31	0.2899514771418197
1107	32-33	0.2668902064178553
1107	34-35	0.2421172217064509
1107	36-37	0.27634326724152913
1107	38-39	0.30478202687769596
1107	40-41	0.2782978549478514
1107	42-43	0.29735689527069553
1107	44-45	0.32828612798842016
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2215	4	-1.7969644108843355
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2216	2	-6.110476007809481
2216	3	-5.514476778416441
2216	4	-3.112679043888754
2216	5	-4.101245943529239
2216	6	-4.48917319714937
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2216	10-11	-0.8043536815125876
2216	12-13	-0.6003043640943133
2216	14-15	-0.8985947144344948
2216	16-17	-0.7239079737021257
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2216	36-37	-1.3762853901255099
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2216	54-55	-1.3353219604318696
2216	56-57	-1.2776618738285137
2216	58-59	-1.196606287593994
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2216	62-63	-1.2326859522197466
2216	64-65	-1.2646582496982397
2216	66-67	-1.371883719201108
2216	68-69	-1.4136993070335322
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2216	76-77	-1.4017961365494003
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2216	80-81	-1.5011941650630618
2216	82-83	-1.4802345178448135
2216	84-85	-1.4331427847857192
2216	86-87	-1.4471061569873633
2216	88-89	-1.3932520899134992
2216	90-91	-1.4065308459424344
2216	92-93	-1.4034229162167087
2216	94-95	-1.4387686830582247
2216	96-97	-1.4854837643442131
2216	98-99	-1.5718916325644336
2216	100	-1.6131521064045629
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	9928.0
3	12815.0
4	16331.0
5	21841.0
6	15231.0
7	10653.0
8	9139.0
9	9652.0
10	9273.0
11	14283.0
12	9863.0
13	12406.0
14	10808.0
15	11112.0
16	12524.0
17	16255.0
18	20701.0
19	32508.0
20	69627.0
21	101761.0
22	45792.0
23	26859.0
24	26057.0
25	27985.0
26	29141.0
27	30917.0
28	36445.0
29	41673.0
30	48170.0
31	60798.0
32	79017.0
33	110773.0
34	166849.0
35	540234.0
36	505404.0
37	853120.0
38	1042536.0
39	377773.0
40	3478.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.994944242833583	46.62213955283485	24.493620222313943	15.88929598201762
2	16.170119105339335	44.36240828692431	23.32103795494909	16.146434652787264
3	17.382583909614215	27.20254854588367	19.713884606017636	35.70098293848448
4	33.67865284317131	28.319630083692804	21.382694795830805	16.619022277305078
5	15.821433722131111	43.26469578356155	22.80347413132206	18.110396362985284
6	36.41786446951044	25.445573728773834	22.848877052858096	15.287684748857632
7	17.560604071850726	25.741048794883266	40.02605513008368	16.67229200318233
8	35.42772201551343	26.2113671085681	22.069132707045867	16.291778168872604
9	35.06651737202136	27.780791350911173	22.07107478750961	15.08161648955786
10-11	15.411346035878932	46.4953818665938	22.164718782284297	15.928553315242965
12-13	15.413701087475767	27.267602169058332	31.330021528073555	25.988675215392348
14-15	15.882470464146795	36.543448537577646	21.789875494589513	25.784205503686053
16-17	25.828024123406944	26.819225461974096	31.132847610523477	16.219902804095483
18-19	16.306490690346063	27.287885445812993	31.079050161823314	25.32657370201763
20-21	26.021936133679425	36.90519879314209	21.58350097729061	15.489364095887877
22-23	25.518242671071395	27.443905386719138	31.123144573094297	15.914707369115174
24-25	16.067623991977793	36.75222633274131	21.580504263361345	25.599645411919553
26-27	16.610985883609935	31.51669988165834	36.05443627197642	15.817877962755306
28-29	25.62503034534255	27.17704881986614	31.41700332557051	15.7809175092208
30-31	22.66167593220763	28.954106718583162	31.97283581363192	16.411381535577288
32-33	18.508996338958834	30.226437628359676	32.89675417447987	18.367811858201623
34-35	18.82998904563989	30.018173162844846	33.087169108436555	18.064668683078708
36-37	18.851184559833314	29.779990619845982	33.67042122161044	17.698403598710268
38-39	20.007448632402287	29.507882298657783	32.98446895392822	17.500200115011708
40-41	17.99056840695371	31.35429862990764	33.15614588274688	17.498987080391775
42-43	18.046502241441704	29.63222541529904	34.073429902513304	18.247842440745952
44-45	18.110392578507607	30.41790543312224	32.89738800035049	18.57431398801966
46-47	18.976038527833687	29.689103249893044	33.623722994805085	17.71113522746818
48-49	18.270395952957145	29.700624201887788	33.55872096657529	18.470258878579784
50-51	19.14890885765292	30.56353893103424	32.62747854088392	17.660073670428922
52-53	19.150044970161645	29.966445941936815	32.87767513305207	18.005833954849468
54-55	18.811667109263926	31.35747425094182	30.76960686312627	19.06125177666799
56-57	19.405357112559905	31.990245183090344	30.08452195235642	18.519875751993332
58-59	21.06897496705604	31.76189484835174	28.683609061667852	18.485521122924364
60-61	20.098011091239858	32.75242103223407	28.503517554060366	18.646050322465708
62-63	19.04013286655918	32.65181181925281	28.35934243107631	19.948712883111693
64-65	19.792934587985663	32.61337728248163	28.903861833451067	18.689826296081637
66-67	19.76914977605124	31.67535237592629	29.843701881321387	18.71179596670108
68-69	20.529647131098503	31.671251872925048	29.3470054629585	18.45209553301795
70-71	18.402925058110927	33.314037439243606	29.572780867998226	18.71025663464724
72-73	18.42002690917517	31.772672609723422	30.25595639525993	19.551344085841475
74-75	18.830502859347263	32.58621128450952	29.01997479988141	19.56331105626181
76-77	19.845994827426473	31.767682912536177	29.439042549272838	18.947279710764512
78-79	19.197278164319286	32.029503669986134	29.023019685485895	19.750198480208688
80-81	20.225403516072497	32.84941580179868	28.170505751455376	18.754674930673453
82-83	20.017366928893377	32.538746290355675	28.61840599767414	18.825480783076813
84-85	19.63090025756726	33.200849441751565	27.65525613012867	19.5129941705525
86-87	19.549438293681778	33.37213893349976	28.27165646628964	18.806766306528818
88-89	21.19095768819218	32.591596135823345	27.522306315317664	18.695139860666814
90-91	20.184747600510526	33.5804947686625	27.363908923163756	18.870848707663217
92-93	19.41751268624701	33.5820924680247	27.26229466654625	19.73810017918204
94-95	20.07677941250009	33.919051777715865	27.04496929984355	18.95919950994049
96-97	20.09884171194363	32.935539013721616	27.926110049309234	19.039509225025522
98-99	21.021795140857964	33.03061188038348	26.94534246636511	19.002250512393445
100	19.334077994763554	34.52488092747633	27.0464588803778	19.094582197382312
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	338390.0
1	179600.0
2	14860.5
3	7248.0
4	5275.0
5	4682.0
6	4903.0
7	6031.0
8	5576.0
9	4812.0
10	4944.0
11	7273.0
12	67563.0
13	73404.5
14	17861.0
15	14437.0
16	15507.0
17	17044.0
18	18164.5
19	19408.0
20	21109.5
21	22838.0
22	24689.0
23	27547.5
24	30840.0
25	34513.0
26	38928.0
27	43474.5
28	48158.0
29	53199.0
30	58303.5
31	63834.5
32	69421.0
33	74527.5
34	80918.5
35	85939.5
36	90191.0
37	94535.0
38	96711.0
39	98760.0
40	100527.0
41	102648.5
42	105252.5
43	106564.5
44	108094.5
45	110344.0
46	113085.0
47	119510.0
48	124559.0
49	141381.5
50	210551.5
51	213139.0
52	142548.5
53	121683.5
54	117682.5
55	112933.5
56	106173.5
57	96567.0
58	84588.5
59	70606.5
60	58780.0
61	47730.5
62	36946.0
63	27450.5
64	20266.0
65	14912.5
66	11591.0
67	9021.5
68	6753.0
69	5167.0
70	4122.5
71	3430.5
72	2633.0
73	1811.5
74	1277.0
75	997.5
76	769.0
77	502.0
78	338.5
79	229.0
80	132.0
81	75.0
82	45.5
83	30.0
84	16.0
85	12.5
86	9.5
87	4.5
88	2.0
89	2.5
90	2.0
91	1.0
92	0.5
93	0.5
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.054913999319602155
2	0.0
3	2.2322763951057786E-5
4	3.571642232169246E-4
5	0.010134534833780235
6	4.464552790211557E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.562593476574045E-4
16-17	3.6832560519245346E-4
18-19	1.1161381975528893E-5
20-21	0.0
22-23	3.348414592658668E-5
24-25	0.003750224343777708
26-27	0.007344189339898011
28-29	0.0011049768155773603
30-31	0.005156558472694348
32-33	0.0029466048415396277
34-35	0.025671178543716453
36-37	0.025168916354817654
38-39	0.02360632287824361
40-41	0.002388535742763183
42-43	0.005558368223813389
44-45	0.006908895442852384
46-47	0.001372849982990054
48-49	0.009196978747835807
50-51	0.010234987271559994
52-53	0.0021206625753504896
54-55	0.010949315717993844
56-57	0.006786120241121567
58-59	0.011931517331840386
60-61	0.007612062507310706
62-63	0.0011049768155773603
64-65	0.013750822593851597
66-67	0.005524884077886802
68-69	0.002410858506714241
70-71	0.008214777133989265
72-73	0.004888685305281655
74-75	0.008661232413010422
76-77	0.007522771451506474
78-79	0.01553664370993622
80-81	0.008650071031034892
82-83	0.01180874213010957
84-85	0.0071321230823629626
86-87	0.012668168542225293
88-89	0.02007932617397648
90-91	0.018092600182332334
92-93	0.017132721332436852
94-95	0.0061610828504919495
96-97	0.010022921014024945
98-99	0.016786718491195455
100	0.016496522559831705
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4479732.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.17198011597375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.94199645506134	30.627064603453885
2	15.963779890288956	13.78375975998673
3	6.5059283233114735	8.426214246298603
4	2.8349413945424735	4.895601340605542
5	1.3031743962610731	2.8130309561514575
6	0.6745343251918239	1.7472589484833916
7	0.39978103637629736	1.2081537267226623
8	0.25548289564334975	0.8823761992548874
9	0.18925788039052682	0.7353573704110048
>10	0.8002305594635278	5.773520891425024
>50	0.057707842864799665	1.786471302592525
>100	0.06597019645733475	4.510604092045788
>500	0.0026471583722463905	0.7880153690090304
>1k	0.004100500184179411	4.496142368721136
>5k	3.114303937351451E-4	0.9844701970396753
>10k+	1.5571519686757255E-4	16.54195862779875
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	353421	7.889333558346793	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	326195	7.281573987015294	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	58586	1.3078014488366714	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9264	0.20679808524259932	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7976	0.1780463652736369	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7082	0.15808981430139124	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6747	0.15061168837778688	No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6610	0.14755346971649197	No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT	6254	0.1396065657499154	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	4998	0.11156917422738682	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4782	0.10674745721395834	No Hit
TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG	4665	0.10413569383168457	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.357463348253868E-4	0.0	0.0	0.0	0.0
2	5.357463348253868E-4	0.0	0.0	0.0	0.0
3	5.357463348253868E-4	0.0	0.0	0.0	0.0
4	5.357463348253868E-4	0.0	0.0	0.0	0.0
5	5.357463348253868E-4	0.0	0.0	0.0	0.0
6	5.357463348253868E-4	0.0	0.0	0.0	0.0
7	5.357463348253868E-4	0.0	0.0	0.0	0.0
8	5.357463348253868E-4	0.0	0.0	0.0	0.0
9	5.357463348253868E-4	0.0	0.0	0.0	0.0
10-11	6.027146266785602E-4	0.0	0.0	0.0	0.0
12-13	7.143284464338492E-4	0.0	0.0	0.0	0.0
14-15	8.594264121157247E-4	0.0	0.0	1.1161381975528893E-5	0.0
16-17	0.0011049768155773606	0.0	0.0	2.2322763951057786E-5	0.0
18-19	0.0014509796568187563	0.0	0.0	2.2322763951057786E-5	0.0
20-21	0.0016965300602803917	0.0	0.0	2.2322763951057786E-5	0.0
22-23	0.001875112171888854	0.0	0.0	2.2322763951057786E-5	0.0
24-25	0.0021095011933749607	2.2322763951057786E-5	0.0	2.2322763951057786E-5	0.0
26-27	0.00232156745091001	2.2322763951057786E-5	0.0	2.2322763951057786E-5	1.1161381975528893E-5
28-29	0.0026340861462248186	3.348414592658668E-5	0.0	2.2322763951057786E-5	2.2322763951057786E-5
30-31	0.0029689276054906855	4.464552790211557E-5	0.0	2.2322763951057786E-5	2.2322763951057786E-5
32-33	0.003147509717099148	4.464552790211557E-5	0.0	2.2322763951057786E-5	2.2322763951057786E-5
34-35	0.00342654426648737	4.464552790211557E-5	0.0	2.2322763951057786E-5	2.2322763951057786E-5
36-37	0.003750224343777708	4.464552790211557E-5	0.0	2.2322763951057786E-5	2.2322763951057786E-5
38-39	0.004107388566994633	4.464552790211557E-5	0.0	2.2322763951057786E-5	2.2322763951057786E-5
40-41	0.004698941811697664	4.464552790211557E-5	0.0	2.2322763951057786E-5	2.2322763951057786E-5
42-43	0.00534630196627834	4.464552790211557E-5	0.0	2.2322763951057786E-5	2.2322763951057786E-5
44-45	0.006071791794687718	4.464552790211557E-5	0.0	2.2322763951057786E-5	2.2322763951057786E-5
46-47	0.006841927150999212	4.464552790211557E-5	0.0	2.2322763951057786E-5	2.2322763951057786E-5
48-49	0.007310705193971425	4.464552790211557E-5	0.0	2.2322763951057786E-5	2.2322763951057786E-5
50-51	0.008337552335720083	4.464552790211557E-5	0.0	2.2322763951057786E-5	2.2322763951057786E-5
52-53	0.009208140129811335	4.464552790211557E-5	0.0	2.2322763951057786E-5	2.2322763951057786E-5
54-55	0.010335439709339755	4.464552790211557E-5	0.0	2.2322763951057786E-5	2.2322763951057786E-5
56-57	0.011496223434794759	4.464552790211557E-5	0.0	2.2322763951057786E-5	2.2322763951057786E-5
58-59	0.012969525855564573	4.464552790211557E-5	0.0	2.2322763951057786E-5	2.2322763951057786E-5
60-61	0.014576764860040736	4.464552790211557E-5	0.0	2.2322763951057786E-5	2.2322763951057786E-5
62-63	0.016742072963293338	4.464552790211557E-5	0.0	2.2322763951057786E-5	2.2322763951057786E-5
64-65	0.019097124560129936	4.464552790211557E-5	0.0	2.2322763951057786E-5	2.2322763951057786E-5
66-67	0.022635282646372594	4.464552790211557E-5	0.0	2.2322763951057786E-5	2.2322763951057786E-5
68-69	0.026698025685465115	4.464552790211557E-5	0.0	2.2322763951057786E-5	2.2322763951057786E-5
70-71	0.031698324810502054	4.464552790211557E-5	0.0	2.2322763951057786E-5	2.2322763951057786E-5
72-73	0.03839515399581939	4.464552790211557E-5	0.0	2.2322763951057786E-5	2.2322763951057786E-5
74-75	0.04777071485526366	4.464552790211557E-5	0.0	2.2322763951057786E-5	2.2322763951057786E-5
76-77	0.05830705944016294	4.464552790211557E-5	0.0	2.2322763951057786E-5	2.2322763951057786E-5
78-79	0.07242620763920699	4.464552790211557E-5	0.0	2.2322763951057786E-5	2.2322763951057786E-5
80-81	0.08975983384720336	4.464552790211557E-5	0.0	2.2322763951057786E-5	2.2322763951057786E-5
82-83	0.11212724332616325	4.464552790211557E-5	0.0	2.2322763951057786E-5	2.2322763951057786E-5
84-85	0.14016463484869185	4.464552790211557E-5	0.0	3.348414592658668E-5	2.2322763951057786E-5
86-87	0.1726777405434075	4.464552790211557E-5	0.0	4.464552790211557E-5	2.2322763951057786E-5
88	0.20349431617784278	4.464552790211557E-5	0.0	4.464552790211557E-5	2.2322763951057786E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	2200	0.0	76.07888	1
GTGGAAT	130010	0.0	62.16071	6
AACGAGT	124350	0.0	61.945663	1
AGTGGAA	129625	0.0	61.526585	5
TGGAATC	131760	0.0	61.49202	7
GAATCAC	132735	0.0	61.079273	9
ACGAGTG	129355	0.0	60.990902	2
GGAATCA	132945	0.0	60.979256	8
CGAGTGG	131970	0.0	60.13844	3
GAGTGGA	134895	0.0	59.2002	4
TCGAGTG	1055	0.0	55.23224	2
AACGTGT	1525	0.0	55.184864	1
ACGTGTG	1785	0.0	52.652042	2
CGTGTGG	2045	0.0	50.094105	3
ATCGAGT	1240	0.0	41.706886	1
AGTACTT	52565	0.0	40.619244	20-21
GAGTACT	52005	0.0	40.07168	20-21
GTACTTT	52165	0.0	39.266308	22-23
TACTTTT	54355	0.0	38.6137	22-23
CATGGGG	23980	0.0	37.757137	24-25
>>END_MODULE