FastQCFastQC Report
Thu 26 May 2016
SRR2132706_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132706_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5045093
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3371746.68320683087507No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA828011.6412185067748009No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG501780.9945901889221863No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA82180.1628909516633291No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT73520.14572575768177118No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT66990.13278248785503063No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT53320.10568685255157834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT22300.079.70121
AACGAGT1154900.066.7856751
GTGGAAT1226400.066.447296
GAATCAC1240450.066.005299
ACGAGTG1200550.065.835632
TGGAATC1241900.065.833627
GGAATCA1245800.065.706738
AGTGGAA1226450.065.49845
CGAGTGG1230550.064.761443
GAGTGGA1265750.063.491374
AACGTGT16450.052.3073961
TCGAGTG14550.048.4472772
CGTGTGG22900.047.4042853
ATCGAGT12850.044.275161
ACGTGTG23300.042.556672
AGTACTT493800.041.3355220-21
GAGTACT487500.041.0937120-21
GTACTTT489250.040.21772822-23
CATGGGG251150.038.00563424-25
TACTTTT532050.037.8657422-23
Produced by FastQC (version 0.11.5)