##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2132706_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5045093 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.838241633999612 33.0 31.0 34.0 25.0 34.0 2 31.585167012778555 34.0 31.0 34.0 28.0 34.0 3 31.79684358643141 34.0 31.0 34.0 28.0 34.0 4 35.7475747226067 37.0 35.0 37.0 35.0 37.0 5 35.411516497317294 37.0 35.0 37.0 33.0 37.0 6 35.76780447852993 37.0 35.0 37.0 35.0 37.0 7 35.954710250138106 37.0 35.0 37.0 35.0 37.0 8 36.09779324186888 37.0 37.0 37.0 35.0 37.0 9 38.01919726752312 39.0 39.0 39.0 37.0 39.0 10-11 38.094962471454934 39.0 39.0 39.0 37.0 39.0 12-13 38.08341005805047 39.0 39.0 39.0 37.0 39.0 14-15 39.60491531077821 41.0 40.0 41.0 37.0 41.0 16-17 39.546416884683794 41.0 40.0 41.0 37.0 41.0 18-19 39.487630852394595 41.0 40.0 41.0 37.0 41.0 20-21 39.37434047301011 41.0 39.5 41.0 36.0 41.0 22-23 39.2843527364114 41.0 39.0 41.0 36.0 41.0 24-25 39.22415057561872 41.0 39.0 41.0 36.0 41.0 26-27 39.15686985353888 41.0 39.0 41.0 36.0 41.0 28-29 39.00222661901376 41.0 39.0 41.0 35.0 41.0 30-31 38.90434804670598 41.0 39.0 41.0 35.0 41.0 32-33 38.77000572635628 41.0 39.0 41.0 35.0 41.0 34-35 38.6816821810817 41.0 39.0 41.0 35.0 41.0 36-37 38.553558180196084 40.5 39.0 41.0 35.0 41.0 38-39 38.161168684105526 40.0 37.0 41.0 35.0 41.0 40-41 37.967928836990716 40.0 37.0 41.0 34.0 41.0 42-43 37.868198663533065 40.0 37.0 41.0 34.0 41.0 44-45 37.7150775020401 40.0 36.5 41.0 34.0 41.0 46-47 37.62503515792474 40.0 36.0 41.0 33.0 41.0 48-49 37.48286552894069 40.0 36.0 41.0 33.0 41.0 50-51 37.24822555302747 40.0 35.5 41.0 33.0 41.0 52-53 36.81279562140876 40.0 35.0 41.0 32.5 41.0 54-55 36.01090564633793 40.0 35.0 41.0 29.0 41.0 56-57 35.40435250648501 39.0 35.0 41.0 25.5 41.0 58-59 35.10677781361018 39.0 35.0 41.0 24.0 41.0 60-61 34.859331829958336 39.0 35.0 41.0 23.0 41.0 62-63 34.58703387628336 39.0 35.0 41.0 22.5 41.0 64-65 34.294679800748966 38.0 35.0 40.0 22.0 41.0 66-67 33.976739180030975 37.0 35.0 40.0 21.5 41.0 68-69 33.680660693469875 37.0 34.0 40.0 20.5 41.0 70-71 33.34243511467479 36.0 34.0 39.0 20.0 41.0 72-73 32.99541554536259 36.0 34.0 39.0 19.5 41.0 74-75 32.63511643888428 35.0 34.0 38.5 18.0 40.0 76-77 32.25576723759106 35.0 34.0 37.0 14.0 39.5 78-79 31.91111065742495 35.0 33.0 37.0 11.0 39.0 80-81 31.580522797102056 35.0 33.0 36.5 7.5 39.0 82-83 31.247065118522094 35.0 33.0 36.0 6.0 38.0 84-85 30.918153342267427 35.0 33.0 36.0 2.0 37.0 86-87 30.808302840007112 35.0 33.0 35.0 2.0 37.0 88-89 30.89338501787777 35.0 33.0 35.0 2.0 36.5 90-91 30.77732937727808 35.0 33.0 35.0 2.0 36.0 92-93 30.643535708856113 35.0 33.0 35.0 2.0 36.0 94-95 30.489194550031087 35.0 33.0 35.0 2.0 36.0 96-97 30.327160074155223 35.0 33.0 35.0 2.0 36.0 98-99 30.210575305549373 35.0 33.0 35.0 2.0 35.0 100 30.13662146564989 35.0 33.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.900315146776137 1101 2 1.4086528130174223 1101 3 1.266251577262551 1101 4 0.6676211047382878 1101 5 0.9860292700339031 1101 6 0.6506975736912182 1101 7 0.44135974865735506 1101 8 0.3403079732947134 1101 9 0.35766299708271276 1101 10-11 0.239680441542788 1101 12-13 0.2867249675717005 1101 14-15 0.37419140601334533 1101 16-17 0.36486304946701864 1101 18-19 0.3120959090250466 1101 20-21 0.41937782928848577 1101 22-23 0.4413678575949831 1101 24-25 0.3858772272488409 1101 26-27 0.4043880138870861 1101 28-29 0.45565867963962603 1101 30-31 0.523034560404561 1101 32-33 0.47467945976232073 1101 34-35 0.4565641088136658 1101 36-37 0.48730834057470673 1101 38-39 0.4475747753793655 1101 40-41 0.42643967280906736 1101 42-43 0.4137906938525404 1101 44-45 0.4874491039081832 1101 46-47 0.4757775822861632 1101 48-49 0.44341569565493444 1101 50-51 0.4575264791655016 1101 52-53 0.5094412016794863 1101 54-55 0.49745025960874756 1101 56-57 0.46110241525814644 1101 58-59 0.39771396287861904 1101 60-61 0.41541685234582815 1101 62-63 0.4673760755240579 1101 64-65 0.4043355438556162 1101 66-67 0.42699980115780534 1101 68-69 0.44601361437108267 1101 70-71 0.3736063320712759 1101 72-73 0.420131513020614 1101 74-75 0.3926049546052539 1101 76-77 0.47406133918621407 1101 78-79 0.45114517013937316 1101 80-81 0.45052437035633375 1101 82-83 0.41521108912082383 1101 84-85 0.36414833869744356 1101 86-87 0.39874430704129793 1101 88-89 0.42668978928245593 1101 90-91 0.4368662115958699 1101 92-93 0.45146254336769687 1101 94-95 0.4294845632547286 1101 96-97 0.3863975099061001 1101 98-99 0.40367080982705517 1101 100 0.4032996682251593 1102 1 1.3410247532122384 1102 2 1.1878915577021552 1102 3 1.0993069526621326 1102 4 0.5680299720284125 1102 5 0.8860653841225528 1102 6 0.5413537402962021 1102 7 0.2994864913212467 1102 8 0.260595142681467 1102 9 0.2552773428733417 1102 10-11 0.20119411079699745 1102 12-13 0.1955316355104486 1102 14-15 0.27766178320872825 1102 16-17 0.28409036856116643 1102 18-19 0.31907167528292746 1102 20-21 0.3572030083531246 1102 22-23 0.33773606079220286 1102 24-25 0.36009283012840854 1102 26-27 0.3165197343773656 1102 28-29 0.404550099774319 1102 30-31 0.42221305972508105 1102 32-33 0.37397377664481724 1102 34-35 0.35227137950953846 1102 36-37 0.38150049922312235 1102 38-39 0.31046592862048783 1102 40-41 0.31242404284393643 1102 42-43 0.35481737338843544 1102 44-45 0.413783855208429 1102 46-47 0.29247419860933377 1102 48-49 0.32429669564426433 1102 50-51 0.32752243024001615 1102 52-53 0.3263081670798087 1102 54-55 0.36068808576147404 1102 56-57 0.2504405756895878 1102 58-59 0.22287189984528055 1102 60-61 0.28464127995248134 1102 62-63 0.21495412808868508 1102 64-65 0.11906675164216551 1102 66-67 0.17869471441170504 1102 68-69 0.1548526998466997 1102 70-71 0.17263849936379927 1102 72-73 0.17959071297300255 1102 74-75 0.12642437396169015 1102 76-77 0.17582154905593939 1102 78-79 0.11378589358786328 1102 80-81 0.08637771722992227 1102 82-83 0.019460425037610918 1102 84-85 0.07494738693440794 1102 86-87 -0.026053677667789543 1102 88-89 -0.023264568629659976 1102 90-91 0.012582572352851429 1102 92-93 -0.006292845473513609 1102 94-95 0.011980208215131682 1102 96-97 0.03278118213989245 1102 98-99 0.0606427109332941 1102 100 0.036802448626421125 1103 1 1.865205898205069 1103 2 1.3463284555454926 1103 3 1.2378739335991895 1103 4 0.6495211900515585 1103 5 0.9560811168133867 1103 6 0.5894245342827418 1103 7 0.3933854231612628 1103 8 0.2669022484546417 1103 9 0.31788002686316474 1103 10-11 0.20539835157388353 1103 12-13 0.22106629270244582 1103 14-15 0.31844667670330296 1103 16-17 0.30021459987938215 1103 18-19 0.3428746313822444 1103 20-21 0.40630048818113096 1103 22-23 0.4051350874490396 1103 24-25 0.3984087580564477 1103 26-27 0.3987373404645709 1103 28-29 0.41930504347744346 1103 30-31 0.39152276860621527 1103 32-33 0.37314285590937857 1103 34-35 0.3835207324709984 1103 36-37 0.4953152774355587 1103 38-39 0.49929551066398403 1103 40-41 0.4117905120002021 1103 42-43 0.42824040077949377 1103 44-45 0.45050577800814295 1103 46-47 0.4735018823280157 1103 48-49 0.4952576920786953 1103 50-51 0.47916600879123195 1103 52-53 0.3903578552031135 1103 54-55 0.284656383830729 1103 56-57 0.3201641139904936 1103 58-59 0.23234933015491777 1103 60-61 0.2892290297175819 1103 62-63 0.24474191341418816 1103 64-65 0.24883299161121641 1103 66-67 0.24084735741831764 1103 68-69 0.289565200286269 1103 70-71 0.13700638461014591 1103 72-73 0.1564615499475508 1103 74-75 0.10938310415902208 1103 76-77 0.1817244816893222 1103 78-79 0.18339223574874453 1103 80-81 0.29371866118808754 1103 82-83 0.2734128548460504 1103 84-85 0.17885686776218535 1103 86-87 0.17664123170408885 1103 88-89 0.22308736643872962 1103 90-91 0.22681937580661682 1103 92-93 0.30789584296169537 1103 94-95 0.31551206913249175 1103 96-97 0.21672239825684514 1103 98-99 0.2335776165552268 1103 100 0.2632065140496991 1104 1 1.9063198384737063 1104 2 1.4002214825657724 1104 3 1.2788711530067403 1104 4 0.6854395403809193 1104 5 0.973526449480687 1104 6 0.6119556914613042 1104 7 0.34070880653683133 1104 8 0.24404675469003223 1104 9 0.25758551169636945 1104 10-11 0.2216173248096993 1104 12-13 0.23488287516622108 1104 14-15 0.3083918275808699 1104 16-17 0.31788680415096593 1104 18-19 0.3002900616152999 1104 20-21 0.33878887142233083 1104 22-23 0.34660990008705994 1104 24-25 0.3946415907830243 1104 26-27 0.3476734753620292 1104 28-29 0.36993332458443007 1104 30-31 0.3843327082362151 1104 32-33 0.360562137318297 1104 34-35 0.3966584982594341 1104 36-37 0.38450123884239673 1104 38-39 0.3514132720736569 1104 40-41 0.36168317702180275 1104 42-43 0.36188862224364016 1104 44-45 0.37430952256038097 1104 46-47 0.4139654500857901 1104 48-49 0.3830983502207488 1104 50-51 0.3901127637655506 1104 52-53 0.4608010783191858 1104 54-55 0.36421417834194614 1104 56-57 0.4087606617245214 1104 58-59 0.38811263755818715 1104 60-61 0.4220599976767474 1104 62-63 0.38530380570298917 1104 64-65 0.38453053658046343 1104 66-67 0.37892302818288925 1104 68-69 0.3739410647754653 1104 70-71 0.37089581337939137 1104 72-73 0.41702240682529634 1104 74-75 0.35597640418005483 1104 76-77 0.37530166288942013 1104 78-79 0.3371824231344398 1104 80-81 0.24177593167065226 1104 82-83 0.33067538411024344 1104 84-85 0.361172145687064 1104 86-87 0.3076471691146736 1104 88-89 0.29743644065478136 1104 90-91 0.32208912805092993 1104 92-93 0.30667382079598937 1104 94-95 0.2793895412751901 1104 96-97 0.3194531467246762 1104 98-99 0.328110596768596 1104 100 0.33865500116396774 1105 1 1.6482112709503838 1105 2 1.3199737499007362 1105 3 1.2156894755757008 1105 4 0.6588909876237139 1105 5 1.0204948801641294 1105 6 0.6585620866682049 1105 7 0.3460979325366438 1105 8 0.27481752948116167 1105 9 0.25933910632528523 1105 10-11 0.25565624790714025 1105 12-13 0.22023304474031136 1105 14-15 0.2800792203878615 1105 16-17 0.28605083312923796 1105 18-19 0.2668362569146723 1105 20-21 0.34893055331973244 1105 22-23 0.3272995431176753 1105 24-25 0.32791379402978293 1105 26-27 0.3409192551218041 1105 28-29 0.4169695039361372 1105 30-31 0.4005126643652872 1105 32-33 0.42840459549154275 1105 34-35 0.3861326467666544 1105 36-37 0.42759351552245306 1105 38-39 0.3765001444887801 1105 40-41 0.38677752476246496 1105 42-43 0.42699323772993836 1105 44-45 0.4289906533229413 1105 46-47 0.47770521611563055 1105 48-49 0.41675758515224004 1105 50-51 0.3786514232965814 1105 52-53 0.42646513813082976 1105 54-55 0.39752083963842466 1105 56-57 0.3715367321341816 1105 58-59 0.26562089878145656 1105 60-61 0.3118106955699389 1105 62-63 0.3981793478406246 1105 64-65 0.3601537353588853 1105 66-67 0.4022903403542699 1105 68-69 0.3886679644896418 1105 70-71 0.40327470580918146 1105 72-73 0.32605241178404754 1105 74-75 0.37762376068745596 1105 76-77 0.3764929793479084 1105 78-79 0.32782883261036133 1105 80-81 0.348566398766458 1105 82-83 0.3827625863246702 1105 84-85 0.34528316236805523 1105 86-87 0.3754227259487557 1105 88-89 0.3354376346356176 1105 90-91 0.3311934249414854 1105 92-93 0.3818513244722226 1105 94-95 0.35336445901601365 1105 96-97 0.37463798638788504 1105 98-99 0.37302675873310776 1105 100 0.38880579279058836 1106 1 1.6155578961530992 1106 2 1.2914108079750868 1106 3 1.1784440756283914 1106 4 0.6576928601407062 1106 5 0.9788472364606875 1106 6 0.6468403010844526 1106 7 0.4102499658516692 1106 8 0.31893988344198476 1106 9 0.26685668799851925 1106 10-11 0.21188167256134705 1106 12-13 0.15575708452279002 1106 14-15 0.25584123506323664 1106 16-17 0.2585725736392348 1106 18-19 0.2595349977778767 1106 20-21 0.2822686298296162 1106 22-23 0.2556619839617582 1106 24-25 0.23562002699299 1106 26-27 0.2595165929555634 1106 28-29 0.3305231825412065 1106 30-31 0.3301528832859688 1106 32-33 0.31218367604008534 1106 34-35 0.2797635169026762 1106 36-37 0.2938198002509438 1106 38-39 0.2989228025942552 1106 40-41 0.2727865774381826 1106 42-43 0.28998340468485395 1106 44-45 0.2623081489018162 1106 46-47 0.3320335440122406 1106 48-49 0.340757770532754 1106 50-51 0.29423649086403003 1106 52-53 0.2853875286244332 1106 54-55 0.297198798053536 1106 56-57 0.22721751733038786 1106 58-59 0.22596579671813544 1106 60-61 0.23878286409863847 1106 62-63 0.31763787376023345 1106 64-65 0.22832792805583324 1106 66-67 0.17969260307224744 1106 68-69 0.2481080295097371 1106 70-71 0.3107651323891787 1106 72-73 0.3129260917561112 1106 74-75 0.2688895080471809 1106 76-77 0.3041523651570799 1106 78-79 0.25079815132123784 1106 80-81 0.24813908908804905 1106 82-83 0.27195861355300366 1106 84-85 0.25691156224902656 1106 86-87 0.2686630854152554 1106 88-89 0.2911055866324972 1106 90-91 0.26744268718194064 1106 92-93 0.2874371999553098 1106 94-95 0.2540683670620645 1106 96-97 0.296261856616745 1106 98-99 0.2755085430063744 1106 100 0.35720672888609073 1107 1 1.6224801686763968 1107 2 1.258595923837607 1107 3 1.205464086572377 1107 4 0.6532007968746925 1107 5 1.0125064001466058 1107 6 0.6492107614353273 1107 7 0.3976558212550074 1107 8 0.3136795974295268 1107 9 0.33028184249130277 1107 10-11 0.2447009533495219 1107 12-13 0.17120475175890704 1107 14-15 0.2347257157989091 1107 16-17 0.2785497282218472 1107 18-19 0.2452419418111944 1107 20-21 0.29590151380598684 1107 22-23 0.2693574107321126 1107 24-25 0.2713615565520371 1107 26-27 0.29135026201959136 1107 28-29 0.31782202942235216 1107 30-31 0.3090589181199519 1107 32-33 0.35321463037024614 1107 34-35 0.3226755458254189 1107 36-37 0.34397071309634697 1107 38-39 0.32585831376559327 1107 40-41 0.35847683521871687 1107 42-43 0.34997451223987497 1107 44-45 0.41922087228517313 1107 46-47 0.41514603323103216 1107 48-49 0.42214986434936463 1107 50-51 0.3843887885668167 1107 52-53 0.36632091689663326 1107 54-55 0.3298808718103814 1107 56-57 0.22367358040015262 1107 58-59 0.1774116825925418 1107 60-61 0.2943940492550752 1107 62-63 0.25904182697279055 1107 64-65 0.2483718381594997 1107 66-67 0.25662385684935884 1107 68-69 0.2358734731350296 1107 70-71 0.24878677375487257 1107 72-73 0.28225641406686464 1107 74-75 0.2626650155183228 1107 76-77 0.33692480672633707 1107 78-79 0.283691789807353 1107 80-81 0.26893966102399247 1107 82-83 0.3040019905934237 1107 84-85 0.279803004336614 1107 86-87 0.19988898495991236 1107 88-89 0.16409327595801315 1107 90-91 0.17327539784618295 1107 92-93 0.2065363486498768 1107 94-95 0.2247212842284121 1107 96-97 0.20037291068817709 1107 98-99 0.1956635097038486 1107 100 0.24104833868663533 1108 1 0.8427123245641432 1108 2 0.9919937651461659 1108 3 1.0759314407337328 1108 4 0.5899903084112808 1108 5 0.9411014962143938 1108 6 0.6162866274263195 1108 7 0.39218121243287385 1108 8 0.34933361868549895 1108 9 0.300729315281437 1108 10-11 0.21009587081199044 1108 12-13 0.18994695615086243 1108 14-15 0.18418639862573372 1108 16-17 0.20620780889173318 1108 18-19 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2215 44-45 -0.8999085164802239 2215 46-47 -0.9138917592633078 2215 48-49 -0.9961154095399536 2215 50-51 -0.9571182977355903 2215 52-53 -0.9530726927304656 2215 54-55 -0.9586896049524611 2215 56-57 -0.8309617190183758 2215 58-59 -0.8610657609357091 2215 60-61 -0.865732527453325 2215 62-63 -0.9451448275270735 2215 64-65 -0.8624549683512086 2215 66-67 -0.8638029743034252 2215 68-69 -0.8330717550069195 2215 70-71 -0.8673117271819208 2215 72-73 -0.8897802783644835 2215 74-75 -0.8736718527099967 2215 76-77 -0.7595134236504144 2215 78-79 -0.8000633535478663 2215 80-81 -0.8071999287697196 2215 82-83 -0.8691911160552337 2215 84-85 -0.8291033424917558 2215 86-87 -0.8633244157817899 2215 88-89 -0.8380974606132519 2215 90-91 -0.834987429496433 2215 92-93 -0.8367989853063946 2215 94-95 -0.8329464365254786 2215 96-97 -0.8410964457552481 2215 98-99 -0.8386562025573028 2215 100 -0.8678954260012546 2216 1 -6.3281793995983655 2216 2 -6.195167243762597 2216 3 -5.514610786081949 2216 4 -3.0913785341234714 2216 5 -3.9610681190105517 2216 6 -4.397764255311056 2216 7 -2.156849625823284 2216 8 -1.443345305520161 2216 9 -1.131646085776751 2216 10-11 -0.9916310994661188 2216 12-13 -0.8539602463773832 2216 14-15 -1.1642914464913972 2216 16-17 -1.0374839828883893 2216 18-19 -1.0316697744092238 2216 20-21 -1.0859600143027848 2216 22-23 -1.0901452527635485 2216 24-25 -1.5776202083684794 2216 26-27 -1.4249788565666819 2216 28-29 -2.1429613218881585 2216 30-31 -1.6169973567959417 2216 32-33 -1.3778343799914126 2216 34-35 -1.3075372921781963 2216 36-37 -1.341053215753142 2216 38-39 -1.3367082536422998 2216 40-41 -1.3651585959451396 2216 42-43 -1.3586712695840575 2216 44-45 -1.4191434418042874 2216 46-47 -1.3481122701399997 2216 48-49 -1.4090014766463383 2216 50-51 -1.3076213055019181 2216 52-53 -1.3198508708570031 2216 54-55 -1.2212720783383375 2216 56-57 -1.240030895541686 2216 58-59 -1.1393240115328709 2216 60-61 -1.1373531210766998 2216 62-63 -1.161705895463868 2216 64-65 -1.190397652606599 2216 66-67 -1.2387849996042277 2216 68-69 -1.2137303690083456 2216 70-71 -1.1247633437065332 2216 72-73 -1.1406363102740897 2216 74-75 -1.2162584770951241 2216 76-77 -1.2455710486439493 2216 78-79 -1.1476382197467245 2216 80-81 -1.2244704822601093 2216 82-83 -1.193676351153787 2216 84-85 -1.1872582112386105 2216 86-87 -1.1699053788724783 2216 88-89 -1.1291476003533951 2216 90-91 -1.0953809314009106 2216 92-93 -1.148852928955396 2216 94-95 -1.1622395357546473 2216 96-97 -1.1882668286646947 2216 98-99 -1.2096821910291098 2216 100 -1.1921850154338962 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5944.0 3 3424.0 4 4305.0 5 5716.0 6 4418.0 7 3641.0 8 3695.0 9 4095.0 10 4828.0 11 10471.0 12 7265.0 13 10423.0 14 9289.0 15 9440.0 16 11613.0 17 14947.0 18 20329.0 19 33348.0 20 73643.0 21 109105.0 22 50702.0 23 30485.0 24 29495.0 25 31968.0 26 32288.0 27 33803.0 28 38866.0 29 45652.0 30 53745.0 31 66279.0 32 84888.0 33 114463.0 34 167038.0 35 337168.0 36 507409.0 37 1001345.0 38 1479182.0 39 586873.0 40 3505.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 14.156648428611291 42.242077016302 26.257901267792416 17.34337328729429 2 17.79971504979493 39.861076864406044 24.727854318613023 17.61135376718601 3 19.059331512818495 24.641508095093588 20.660867896786044 35.63829249530187 4 33.47399179517633 25.508054280991722 22.5420166693062 18.475937254525746 5 17.687825681702712 38.60709973474664 23.770970645704892 19.93410393784575 6 36.27362989614461 22.73897086514973 23.779982604876185 17.20741663382947 7 19.126743550614428 22.878706101156112 39.39538874704589 18.599161601183567 8 35.21166012202352 23.18851208491102 23.396714391588024 18.203113401477435 9 34.94514372678561 24.82188138058109 23.220126962971744 17.012847929661554 10-11 17.211932465863363 41.666119930792156 23.441262628855405 17.680684974489072 12-13 17.1522408011111 24.337955712610253 31.844824267857895 26.664979218420747 14-15 17.485337715682604 32.732547525939594 23.285198055058316 26.496916703319485 16-17 26.35087423369581 23.971861273433866 31.816193830994205 17.86107066187612 18-19 17.746184262609233 24.231535474172627 31.85639987211336 26.165880391104785 20-21 26.48808456058194 32.8951716053599 23.242564606836783 17.374179227221383 22-23 26.072787777981826 24.33893418979616 31.928965014806966 17.659313017415045 24-25 17.5473495369533 32.74280974564122 23.270444427904096 26.439396289501392 26-27 17.963039004604887 28.267354351815815 36.16632775000468 17.60327889357462 28-29 26.639936713916494 24.024361019277002 31.78773869792966 17.54796356887684 30-31 23.897071176069883 25.70760642652994 32.25858117650301 18.136741220897175 32-33 20.002174456420583 26.862713305354134 33.25922275769836 19.875889480526922 34-35 20.135247046526562 26.64649936050289 33.4668237702196 19.75142982275095 36-37 20.16847224569687 26.43606604667162 34.073712431407905 19.321749276223603 38-39 20.937615763124892 26.294750300581416 33.47053003539173 19.29710390090196 40-41 19.606173445905043 27.81863371815288 33.274026642817134 19.301166193124946 42-43 19.71847150992139 26.413180350193493 33.983189562681844 19.88515857720328 44-45 19.54127000944851 27.227885524848254 33.20543369138824 20.025410774315 46-47 20.34609325717253 26.41588177666029 33.779617653223895 19.45840731294329 48-49 19.727814426640204 26.625711038424594 33.70820928681524 19.93826524811996 50-51 20.428628349634657 27.330927450848236 32.84324958668511 19.39719461283199 52-53 20.40515259897546 26.893602125515464 33.062001336772255 19.639243938736822 54-55 20.293284826532417 27.917931076043768 31.29326976066683 20.495514336756983 56-57 20.675972077819747 28.465776695901766 30.62656413072243 20.23168709555606 58-59 22.10938909340327 28.43667421949184 29.37046904828456 20.08346763882033 60-61 21.41736376013899 29.062689896210802 29.266646665463426 20.253299678186785 62-63 20.476572087098013 29.158980057399077 29.17983226657369 21.18461558892922 64-65 21.045952009610566 29.036539680510103 29.515551504897214 20.40195680498211 66-67 21.043670864497262 28.26816890486653 30.33376955567167 20.354390674964538 68-69 21.61857790929739 28.334102582984066 29.938159067481116 20.109160440237428 70-71 20.08744475110468 29.55000435112617 30.04705361360533 20.315497284163825 72-73 20.1050046126467 28.299360572594573 30.59159027486776 21.00404453989097 74-75 20.323203722256427 28.86037217013877 29.7423901050651 21.074034002539705 76-77 21.164907263104936 28.138599663567838 30.257416592694675 20.439076480632554 78-79 20.6472781958703 28.486423788413862 29.89277284749753 20.973525168218305 80-81 21.128322936758956 29.16888558376376 29.33287978717426 20.369911692303027 82-83 21.197193408281905 28.78555506646223 29.63728779657533 20.37996372868054 84-85 20.836185318900053 29.46732937791926 28.72506978072765 20.97141552245303 86-87 20.813495442199702 29.664606241856116 29.043842226741617 20.478056089202564 88-89 22.10607400147042 29.101968932399537 28.43393493366893 20.358022132461112 90-91 21.480509048145628 29.833614115749498 28.345827456991522 20.340049379113356 92-93 20.757036454488713 29.895255953389288 28.21849478668626 21.129212805435735 94-95 21.209399234141177 30.142350708472932 28.025772707468334 20.62247734991756 96-97 21.20857195530261 29.341076281719623 28.68736606939315 20.76298569358461 98-99 21.883546595427422 29.57762478208985 27.857825887373945 20.681002735108784 100 20.843275279504482 30.559679034725246 27.913832765958457 20.68321291981181 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 84949.0 1 45479.0 2 4464.0 3 2439.5 4 1923.0 5 1789.0 6 1832.0 7 2147.5 8 1998.0 9 1849.5 10 1955.5 11 2998.0 12 32077.5 13 37876.0 14 15117.0 15 14984.5 16 16407.5 17 18243.5 18 20236.5 19 22355.0 20 24380.5 21 26947.5 22 30300.0 23 34602.5 24 39452.0 25 44975.0 26 53011.5 27 61510.5 28 67043.5 29 72056.0 30 77838.0 31 84331.0 32 92233.5 33 101691.5 34 112847.5 35 120204.0 36 126993.5 37 133157.0 38 136555.0 39 140111.5 40 142906.5 41 145989.5 42 147428.5 43 149076.0 44 153573.5 45 156453.0 46 155795.5 47 158844.0 48 164112.5 49 176136.0 50 226682.0 51 223128.0 52 161117.5 53 136358.0 54 126358.5 55 118837.5 56 109616.0 57 98825.5 58 91783.0 59 78795.5 60 59866.5 61 46553.5 62 35671.0 63 27504.0 64 20971.0 65 15541.0 66 12105.5 67 9523.0 68 7090.0 69 5173.5 70 3833.0 71 2721.5 72 2118.5 73 1722.5 74 1253.5 75 920.0 76 677.5 77 428.5 78 299.5 79 181.0 80 98.5 81 59.0 82 28.5 83 15.5 84 12.0 85 7.5 86 4.0 87 2.5 88 1.5 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.053636275882327646 2 1.9821240163461803E-5 3 0.0 4 2.3785488196154163E-4 5 0.009553837758788588 6 1.9821240163461803E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.1892744098077082E-4 16-17 3.4687170286058154E-4 18-19 0.0 20-21 0.0 22-23 2.9731860245192704E-5 24-25 0.0037957674913029356 26-27 0.006788774755985668 28-29 0.001308201850788479 30-31 0.005678785306831807 32-33 0.0030524709851731174 34-35 0.025490114850211878 36-37 0.024489142221957055 38-39 0.02217005712283203 40-41 0.0025965824614134964 42-43 0.006154495070754891 44-45 0.0075816243625241395 46-47 0.0012982912307067482 48-49 0.008850183732985696 50-51 0.010168296203855905 52-53 0.0020812302171634893 54-55 0.011436855574317461 56-57 0.0070960039785193255 58-59 0.012348632621836704 60-61 0.007859121724812604 62-63 0.0012685593704615554 64-65 0.012794610525514595 66-67 0.005252628643317378 68-69 0.002646135561822151 70-71 0.00836456334898088 72-73 0.005292271123644302 74-75 0.008146529707182801 76-77 0.007750104903913565 78-79 0.014965036323413661 80-81 0.007978049165793377 82-83 0.012120688359956892 84-85 0.007175288939173173 86-87 0.01316130346853864 88-89 0.01909776489749545 90-91 0.01694716033975984 92-93 0.016739037318043493 94-95 0.005926550808875079 96-97 0.009970083802221287 98-99 0.017234568322130037 100 0.0160353832922406 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5045093.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.34652570855327 #Duplication Level Percentage of deduplicated Percentage of total 1 80.01201453208566 45.88411048357388 2 13.521734750516993 15.50849018989522 3 3.367386200813946 5.793236980068135 4 1.0733297845203067 2.4620693632699884 5 0.4878345659111873 1.3987808737773413 6 0.2990799151373695 1.0290716425402253 7 0.20504840023164023 0.8231169348767035 8 0.15421356284999688 0.7074889637267954 9 0.11618543609688586 0.5996547988280585 >10 0.6343100548142988 6.758530418661042 >50 0.11122433700443875 4.291852570470016 >100 0.013440455616980707 1.3443566214238443 >500 0.0015265479708111345 0.5777785578433581 >1k 0.0024285967541607008 2.9181774415508346 >5k 1.3877695738055207E-4 0.5491478643792111 >10k+ 1.0408271803541407E-4 9.354136295115367 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 337174 6.68320683087507 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 82801 1.6412185067748009 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG 50178 0.9945901889221863 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA 8218 0.1628909516633291 No Hit ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 7352 0.14572575768177118 No Hit TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 6699 0.13278248785503063 No Hit CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 5332 0.10568685255157834 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.5767612212500345E-4 0.0 0.0 0.0 0.0 2 2.5767612212500345E-4 0.0 0.0 0.0 0.0 3 2.5767612212500345E-4 0.0 0.0 0.0 0.0 4 2.5767612212500345E-4 1.9821240163461803E-5 0.0 0.0 0.0 5 2.5767612212500345E-4 1.9821240163461803E-5 0.0 1.9821240163461803E-5 0.0 6 2.5767612212500345E-4 1.9821240163461803E-5 0.0 1.9821240163461803E-5 0.0 7 2.5767612212500345E-4 1.9821240163461803E-5 0.0 1.9821240163461803E-5 0.0 8 2.5767612212500345E-4 1.9821240163461803E-5 0.0 1.9821240163461803E-5 0.0 9 2.5767612212500345E-4 1.9821240163461803E-5 0.0 1.9821240163461803E-5 0.0 10-11 2.6758674220673433E-4 1.9821240163461803E-5 0.0 1.9821240163461803E-5 0.0 12-13 2.7749736228846526E-4 1.9821240163461803E-5 0.0 2.9731860245192704E-5 0.0 14-15 3.0722922253365796E-4 1.9821240163461803E-5 0.0 3.9642480326923605E-5 0.0 16-17 3.468717028605816E-4 1.9821240163461803E-5 0.0 3.9642480326923605E-5 0.0 18-19 4.1624604343269787E-4 2.9731860245192704E-5 0.0 3.9642480326923605E-5 0.0 20-21 5.05441624168276E-4 3.9642480326923605E-5 0.0 3.9642480326923605E-5 0.0 22-23 6.640115454759704E-4 3.9642480326923605E-5 0.0 7.928496065384721E-5 0.0 24-25 8.622239471105884E-4 5.946372049038541E-5 0.0 9.910620081730901E-5 0.0 26-27 0.0011099894491538608 5.946372049038541E-5 0.0 1.1892744098077082E-4 0.0 28-29 0.0013181124708702098 5.946372049038541E-5 0.0 1.2883806106250172E-4 0.0 30-31 0.0014865930122596352 5.946372049038541E-5 0.0 1.5856992130769442E-4 0.0 32-33 0.0017541797544663696 5.946372049038541E-5 0.0 1.7839116147115624E-4 0.0 34-35 0.0020019452565096423 5.946372049038541E-5 0.0 1.7839116147115624E-4 0.0 36-37 0.0022596213786346456 5.946372049038541E-5 0.0 1.7839116147115624E-4 0.0 38-39 0.0026560461819038817 5.946372049038541E-5 0.0 1.7839116147115624E-4 0.0 40-41 0.0031416665659086958 5.946372049038541E-5 0.0 1.9821240163461803E-4 0.0 42-43 0.0036669294302404338 5.946372049038541E-5 0.0 1.9821240163461803E-4 0.0 44-45 0.004281387875307749 5.946372049038541E-5 0.0 1.9821240163461803E-4 0.0 46-47 0.004866114460129873 5.946372049038541E-5 0.0 1.9821240163461803E-4 0.0 48-49 0.005440930424870266 5.946372049038541E-5 0.0 1.9821240163461803E-4 0.0 50-51 0.006213958791245275 5.946372049038541E-5 0.0 1.9821240163461803E-4 0.0 52-53 0.007214931419500097 5.946372049038541E-5 0.0 2.1803364179807984E-4 0.0 54-55 0.008067244746528954 5.946372049038541E-5 0.0 2.1803364179807984E-4 0.0 56-57 0.009266429776418393 5.946372049038541E-5 0.0 2.1803364179807984E-4 0.0 58-59 0.010554810387043409 5.946372049038541E-5 0.0 2.1803364179807984E-4 0.0 60-61 0.012150420220202084 5.946372049038541E-5 0.0 2.1803364179807984E-4 0.0 62-63 0.014241561057447305 5.946372049038541E-5 0.0 2.1803364179807984E-4 0.0 64-65 0.01706608778074061 5.946372049038541E-5 0.0 2.1803364179807984E-4 0.0 66-67 0.02022757558681277 5.946372049038541E-5 0.0 2.1803364179807984E-4 0.0 68-69 0.024370214780976285 5.946372049038541E-5 0.0 2.1803364179807984E-4 0.0 70-71 0.02943454164274078 5.946372049038541E-5 0.0 2.1803364179807984E-4 0.0 72-73 0.035836802215538935 5.946372049038541E-5 0.0 2.1803364179807984E-4 0.0 74-75 0.04399324254280347 5.946372049038541E-5 0.0 2.1803364179807984E-4 0.0 76-77 0.05535081315646709 5.946372049038541E-5 0.0 2.1803364179807984E-4 0.0 78-79 0.06916621755039996 5.946372049038541E-5 0.0 2.1803364179807984E-4 0.0 80-81 0.08691613811678 5.946372049038541E-5 0.0 2.1803364179807984E-4 0.0 82-83 0.10963127934410724 5.946372049038541E-5 0.0 2.1803364179807984E-4 0.0 84-85 0.13878832362455956 5.946372049038541E-5 0.0 2.1803364179807984E-4 0.0 86-87 0.17243487880203595 5.946372049038541E-5 0.0 2.1803364179807984E-4 0.0 88 0.2015027275017527 5.946372049038541E-5 0.0 2.1803364179807984E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGAGT 2230 0.0 79.7012 1 AACGAGT 115490 0.0 66.785675 1 GTGGAAT 122640 0.0 66.44729 6 GAATCAC 124045 0.0 66.00529 9 ACGAGTG 120055 0.0 65.83563 2 TGGAATC 124190 0.0 65.83362 7 GGAATCA 124580 0.0 65.70673 8 AGTGGAA 122645 0.0 65.4984 5 CGAGTGG 123055 0.0 64.76144 3 GAGTGGA 126575 0.0 63.49137 4 AACGTGT 1645 0.0 52.307396 1 TCGAGTG 1455 0.0 48.447277 2 CGTGTGG 2290 0.0 47.404285 3 ATCGAGT 1285 0.0 44.27516 1 ACGTGTG 2330 0.0 42.55667 2 AGTACTT 49380 0.0 41.33552 20-21 GAGTACT 48750 0.0 41.09371 20-21 GTACTTT 48925 0.0 40.217728 22-23 CATGGGG 25115 0.0 38.005634 24-25 TACTTTT 53205 0.0 37.86574 22-23 >>END_MODULE