FastQCFastQC Report
Thu 26 May 2016
SRR2132705_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132705_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5579720
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3357346.017040281591191No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA737481.32171506813962No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG203200.3641759801567104No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT73650.13199587076053995No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT64450.11550758819438968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT20150.082.146091
AACGAGT921250.077.336241
ACGAGTG960000.076.021292
CGAGTGG983900.074.776463
GTGGAAT1011950.073.958916
GAATCAC1020700.073.668849
TGGAATC1021400.073.526357
GGAATCA1027300.073.140668
AGTGGAA1010500.073.05925
GAGTGGA1019450.072.537654
TCGAGTG11550.047.1966362
CGTGTGG20550.047.107543
ATCGAGT9900.046.5489771
AACGTGT17750.044.7721181
ACGTGTG21750.042.9960822
GAGTACT493100.041.64332620-21
AGTACTT508100.041.20936620-21
GTACTTT500050.040.28179622-23
CATGGGG233500.038.11220624-25
TACTTTT544050.037.8878422-23
Produced by FastQC (version 0.11.5)