FastQCFastQC Report
Thu 26 May 2016
SRR2132704_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132704_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5644697
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4074887.21895258505461No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3008445.3296749143488125No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG838711.485837060873241No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC109930.1947491601409252No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT94050.1666165606408989No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT85210.15095584404264747No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT81340.1440998515952229No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT72920.129183196192816No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68710.12172486849161257No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA65980.11688847071862317No Hit
TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG60540.10725110665816076No Hit
AGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTC58830.10422171464650803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT27200.072.961841
GTGGAAT1634550.059.280476
AACGAGT1573250.058.600211
TGGAATC1660350.058.5185787
GAATCAC1666050.058.352219
AGTGGAA1641900.058.1704675
GGAATCA1672350.058.168928
ACGAGTG1631500.057.8273742
CGAGTGG1668350.056.9332243
GAGTGGA1708100.056.007284
CGTGTGG26050.051.058183
AACGTGT21850.048.641751
ACGTGTG26100.046.9987872
AGTACTT590800.041.05629720-21
ATCTGCG29850.040.9376994
GAGTACT586500.040.4999520-21
TCGAGTG15150.040.018772
GTACTTT587100.039.77123622-23
TACTTTT611500.039.0873122-23
ATCGAGT13400.038.604691
Produced by FastQC (version 0.11.5)