##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132704_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5644697
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.860675604022678	33.0	31.0	34.0	25.0	34.0
2	31.606529455876906	34.0	31.0	34.0	28.0	34.0
3	31.809010651944646	34.0	31.0	34.0	28.0	34.0
4	35.74776697491468	37.0	35.0	37.0	35.0	37.0
5	35.382588117661584	37.0	35.0	37.0	33.0	37.0
6	35.708707482438825	37.0	35.0	37.0	35.0	37.0
7	35.88350623603003	37.0	35.0	37.0	35.0	37.0
8	35.999340797211964	37.0	37.0	37.0	35.0	37.0
9	37.88763524419468	39.0	39.0	39.0	37.0	39.0
10-11	37.92911931322443	39.0	39.0	39.0	37.0	39.0
12-13	37.86645075900442	39.0	39.0	39.0	37.0	39.0
14-15	39.3547325923783	41.0	40.0	41.0	37.0	41.0
16-17	39.22171083762335	41.0	39.5	41.0	37.0	41.0
18-19	38.977443430533114	41.0	39.0	41.0	36.0	41.0
20-21	38.791946139890236	41.0	39.0	41.0	35.0	41.0
22-23	38.668341896828125	40.5	39.0	41.0	35.0	41.0
24-25	38.57465458287663	41.0	39.0	41.0	35.0	41.0
26-27	38.48811176224339	41.0	39.0	41.0	35.0	41.0
28-29	38.32672258581816	41.0	38.0	41.0	35.0	41.0
30-31	38.21137804208091	41.0	38.0	41.0	35.0	41.0
32-33	38.05917492116937	40.5	38.0	41.0	35.0	41.0
34-35	37.9554280770075	40.0	38.0	41.0	34.0	41.0
36-37	37.78927504877587	40.0	38.0	41.0	34.0	41.0
38-39	37.36791416793497	40.0	36.0	41.0	34.0	41.0
40-41	37.16776551513748	40.0	35.0	41.0	33.0	41.0
42-43	37.05340074055348	40.0	35.0	41.0	33.0	41.0
44-45	36.88950283779626	40.0	35.0	41.0	33.0	41.0
46-47	36.7776670740697	40.0	35.0	41.0	33.0	41.0
48-49	36.60989969877923	40.0	35.0	41.0	33.0	41.0
50-51	36.3514068159903	39.0	35.0	41.0	32.0	41.0
52-53	35.87049366865928	39.0	35.0	41.0	30.5	41.0
54-55	35.031511966009866	39.0	35.0	41.0	26.0	41.0
56-57	34.42728945415493	39.0	35.0	41.0	21.0	41.0
58-59	34.142798807447065	38.0	35.0	41.0	18.0	41.0
60-61	33.90091168755382	37.5	35.0	40.5	16.0	41.0
62-63	33.645135691074294	37.0	35.0	40.0	13.5	41.0
64-65	33.33936081954444	36.5	34.5	40.0	10.0	41.0
66-67	33.01460034081546	36.0	34.0	39.5	8.0	41.0
68-69	32.75916873837515	35.5	34.0	39.0	7.0	41.0
70-71	32.4646796807694	35.0	34.0	39.0	6.0	41.0
72-73	32.15931572589281	35.0	34.0	38.0	2.0	40.0
74-75	31.8361330997926	35.0	33.0	37.0	2.0	40.0
76-77	31.493860963662	35.0	33.0	37.0	2.0	39.0
78-79	31.19291620081645	35.0	33.0	36.0	2.0	39.0
80-81	30.898731588249998	35.0	33.0	36.0	2.0	38.5
82-83	30.582849531161727	35.0	32.5	35.5	2.0	37.0
84-85	30.2922079785682	35.0	32.0	35.0	2.0	37.0
86-87	30.20479965532251	35.0	32.5	35.0	2.0	36.5
88-89	30.295718884468023	35.0	33.0	35.0	2.0	36.0
90-91	30.185121716896408	35.0	33.0	35.0	2.0	36.0
92-93	30.063285682118988	35.0	33.0	35.0	2.0	36.0
94-95	29.90695709619135	35.0	33.0	35.0	2.0	35.5
96-97	29.720109954529	35.0	33.0	35.0	2.0	35.0
98-99	29.608399086788893	35.0	33.0	35.0	2.0	35.0
100	29.5425692468524	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.826243437376359
1101	2	1.3392013175676816
1101	3	1.230752015213426
1101	4	0.6363241675935996
1101	5	0.9295869694121279
1101	6	0.5465176764850099
1101	7	0.33235801831261114
1101	8	0.25474018571076584
1101	9	0.28567865801760206
1101	10-11	0.22444850209041078
1101	12-13	0.2931082332531716
1101	14-15	0.4250760040224293
1101	16-17	0.41082784491189983
1101	18-19	0.43190173166919266
1101	20-21	0.4756959225425703
1101	22-23	0.45155140544943606
1101	24-25	0.5005561098606677
1101	26-27	0.47426655229070747
1101	28-29	0.4850616443750795
1101	30-31	0.5309852387587171
1101	32-33	0.45043535480076713
1101	34-35	0.4987369081067712
1101	36-37	0.535542067848553
1101	38-39	0.4914637853729431
1101	40-41	0.4469028449106389
1101	42-43	0.4608398809222152
1101	44-45	0.5254470823909259
1101	46-47	0.4757279838198727
1101	48-49	0.43194587408835616
1101	50-51	0.42873032321470106
1101	52-53	0.4669909493510431
1101	54-55	0.3011032356563845
1101	56-57	0.3437896433407417
1101	58-59	0.34160735258536334
1101	60-61	0.3298915212381459
1101	62-63	0.35321182363157533
1101	64-65	0.373331963454298
1101	66-67	0.38813184595163364
1101	68-69	0.45623594833330827
1101	70-71	0.3212016585691515
1101	72-73	0.3550269071365051
1101	74-75	0.33141794724637563
1101	76-77	0.3516070810453158
1101	78-79	0.3848601931910913
1101	80-81	0.38942463949835826
1101	82-83	0.38400434634148795
1101	84-85	0.3617724111476015
1101	86-87	0.4447806739564868
1101	88-89	0.41446341489873006
1101	90-91	0.4422944768494297
1101	92-93	0.3938668940628425
1101	94-95	0.41254435187677885
1101	96-97	0.491338605147579
1101	98-99	0.49145011209840916
1101	100	0.42498009584112495
1102	1	1.3010810153249999
1102	2	1.1291045494158176
1102	3	1.104589540912464
1102	4	0.5172861196730807
1102	5	0.8338761641856891
1102	6	0.5004691320568497
1102	7	0.28069206675340297
1102	8	0.24314506367876731
1102	9	0.2354652797341643
1102	10-11	0.2416943199987429
1102	12-13	0.25684899080034995
1102	14-15	0.35544950460253233
1102	16-17	0.3828895619024806
1102	18-19	0.43401429721820506
1102	20-21	0.5073807422998797
1102	22-23	0.530359541390375
1102	24-25	0.5404105711230756
1102	26-27	0.48900372863809594
1102	28-29	0.5537339064680111
1102	30-31	0.5767547754557398
1102	32-33	0.5320219684696923
1102	34-35	0.5113139623290692
1102	36-37	0.5545048900683724
1102	38-39	0.5088458211701834
1102	40-41	0.4892336971171005
1102	42-43	0.47045297561642485
1102	44-45	0.5090207939255862
1102	46-47	0.41566438000948125
1102	48-49	0.5052696549245255
1102	50-51	0.5524843825326755
1102	52-53	0.5498018510033091
1102	54-55	0.45372449698925266
1102	56-57	0.45385731815878216
1102	58-59	0.3842975259107675
1102	60-61	0.4376760600058489
1102	62-63	0.394391074001625
1102	64-65	0.40338830293978134
1102	66-67	0.41964479969202984
1102	68-69	0.4068551232854887
1102	70-71	0.35929401270202277
1102	72-73	0.37419857333514983
1102	74-75	0.3934418120165155
1102	76-77	0.4072309014075799
1102	78-79	0.4288340079894972
1102	80-81	0.3647788450465832
1102	82-83	0.3682823808058089
1102	84-85	0.4071006231619059
1102	86-87	0.3717744402957486
1102	88-89	0.40339452958866673
1102	90-91	0.39614302901636833
1102	92-93	0.42523229629770043
1102	94-95	0.37767268385067965
1102	96-97	0.34791647408618687
1102	98-99	0.38329028562543144
1102	100	0.3733694653720221
1103	1	1.7752762036888363
1103	2	1.2815044848558976
1103	3	1.1835681121895334
1103	4	0.5701652998283961
1103	5	0.8243370053470755
1103	6	0.4127023584089642
1103	7	0.22790170619884975
1103	8	0.1696381443667363
1103	9	0.2093408666726475
1103	10-11	0.15290644407309628
1103	12-13	0.21865512909644025
1103	14-15	0.33979351716719464
1103	16-17	0.35644510828884535
1103	18-19	0.36238488507139976
1103	20-21	0.4347726740333968
1103	22-23	0.45982139237759867
1103	24-25	0.42298576453769954
1103	26-27	0.3470506187589635
1103	28-29	0.4217232974969676
1103	30-31	0.44071840150931507
1103	32-33	0.3542586432096684
1103	34-35	0.2801130616718055
1103	36-37	0.35994686369292594
1103	38-39	0.4255737004497604
1103	40-41	0.3851386329681361
1103	42-43	0.47347968579143895
1103	44-45	0.4525164732497089
1103	46-47	0.3915489801489471
1103	48-49	0.42879146311925354
1103	50-51	0.4609398262462321
1103	52-53	0.47444252103451845
1103	54-55	0.4190411471513755
1103	56-57	0.3012399818320972
1103	58-59	0.2957822814182407
1103	60-61	0.38131310500424576
1103	62-63	0.33220152833377625
1103	64-65	0.29844084404204807
1103	66-67	0.3380609887876389
1103	68-69	0.3866746885864458
1103	70-71	0.33150141508663467
1103	72-73	0.373075171093717
1103	74-75	0.3461983266690929
1103	76-77	0.35172805487194125
1103	78-79	0.349090999881728
1103	80-81	0.40620452226289316
1103	82-83	0.45529210820917143
1103	84-85	0.39716104700373833
1103	86-87	0.39486272267049216
1103	88-89	0.3616126708595573
1103	90-91	0.37490654417800684
1103	92-93	0.35876968272129517
1103	94-95	0.34296279584291867
1103	96-97	0.33774042402639637
1103	98-99	0.3780028349070541
1103	100	0.41632742983887994
1104	1	1.884493276220919
1104	2	1.3520028281596232
1104	3	1.262098411099842
1104	4	0.6717603106044692
1104	5	0.9901551816719305
1104	6	0.5534630035637562
1104	7	0.32694117484750507
1104	8	0.2235707838011436
1104	9	0.24598268289517478
1104	10-11	0.22180115321967264
1104	12-13	0.28085066709471107
1104	14-15	0.4113622297782271
1104	16-17	0.38429315298440514
1104	18-19	0.4368570334388622
1104	20-21	0.4341122615210651
1104	22-23	0.49103822068544645
1104	24-25	0.4824619791125997
1104	26-27	0.4634123828131891
1104	28-29	0.5029893483157508
1104	30-31	0.5193756592489578
1104	32-33	0.49079074736500417
1104	34-35	0.5117035908074996
1104	36-37	0.5718248472568916
1104	38-39	0.5024227547732849
1104	40-41	0.5275803968209374
1104	42-43	0.5658562392589204
1104	44-45	0.5539739505393726
1104	46-47	0.6031318804248897
1104	48-49	0.575578718643257
1104	50-51	0.5944910730486583
1104	52-53	0.6191254197998006
1104	54-55	0.5167235977526161
1104	56-57	0.5770138830748692
1104	58-59	0.5176873058910161
1104	60-61	0.549855829136618
1104	62-63	0.44927871829489874
1104	64-65	0.4604850637330671
1104	66-67	0.4426884713142769
1104	68-69	0.4323741392078375
1104	70-71	0.4760769936714411
1104	72-73	0.4347780433720061
1104	74-75	0.429412254499578
1104	76-77	0.4846582294041326
1104	78-79	0.47150266372848293
1104	80-81	0.4211441208287674
1104	82-83	0.46055016760853107
1104	84-85	0.4785899349526552
1104	86-87	0.45498864921187376
1104	88-89	0.40787434074897533
1104	90-91	0.4271150109534396
1104	92-93	0.4493247515069214
1104	94-95	0.467439224428734
1104	96-97	0.48456720862566627
1104	98-99	0.47943444205350616
1104	100	0.46831370292688135
1105	1	1.5856012582845729
1105	2	1.2493678322577146
1105	3	1.1961029831328744
1105	4	0.603371222409308
1105	5	0.9876660926217227
1105	6	0.5952614383478476
1105	7	0.24661813988727488
1105	8	0.12490639032536421
1105	9	0.07683379805349233
1105	10-11	0.06054250660496052
1105	12-13	0.09325129897092665
1105	14-15	0.1715196735927904
1105	16-17	0.18639808376872935
1105	18-19	0.2101611029513606
1105	20-21	0.2910148648804025
1105	22-23	0.34758384390742236
1105	24-25	0.32805797714843976
1105	26-27	0.33041737137394733
1105	28-29	0.3881076739475944
1105	30-31	0.3693759646211845
1105	32-33	0.3625257685517269
1105	34-35	0.3535269897714315
1105	36-37	0.3358261792905637
1105	38-39	0.3854409286962053
1105	40-41	0.36146686614385715
1105	42-43	0.3999288024190619
1105	44-45	0.4062936648819786
1105	46-47	0.4575337447834258
1105	48-49	0.38789267061187616
1105	50-51	0.3627366210269045
1105	52-53	0.3816738060297098
1105	54-55	0.4300062820478132
1105	56-57	0.4884689756454037
1105	58-59	0.5076980611009745
1105	60-61	0.4702138353246781
1105	62-63	0.5171901432472339
1105	64-65	0.4560300951436531
1105	66-67	0.471916471993417
1105	68-69	0.5338756850020658
1105	70-71	0.52362729763896
1105	72-73	0.4634371355858633
1105	74-75	0.4671460627478119
1105	76-77	0.5061231946588833
1105	78-79	0.5025043732022318
1105	80-81	0.515066755384936
1105	82-83	0.5227787680387408
1105	84-85	0.5038053750612228
1105	86-87	0.4907215822632871
1105	88-89	0.45042512109717237
1105	90-91	0.38325236677194496
1105	92-93	0.42579029391518475
1105	94-95	0.43100248472924463
1105	96-97	0.46059658787721247
1105	98-99	0.4249118640522802
1105	100	0.47321266747072244
1106	1	1.5852677922492795
1106	2	1.2624649372249657
1106	3	1.1451603006427504
1106	4	0.6581075936556928
1106	5	0.994948267432747
1106	6	0.6380786778957699
1106	7	0.35533848175206373
1106	8	0.21530775503725152
1106	9	0.16099530902193493
1106	10-11	0.09057946784696469
1106	12-13	0.11299595184614475
1106	14-15	0.2069646968947012
1106	16-17	0.24994363166525346
1106	18-19	0.281047535506751
1106	20-21	0.29592961670620355
1106	22-23	0.2857487882513752
1106	24-25	0.24716096973985202
1106	26-27	0.2605341101534222
1106	28-29	0.35723569050530557
1106	30-31	0.3814619473470344
1106	32-33	0.39327391551846347
1106	34-35	0.30393892638627307
1106	36-37	0.31286186655836445
1106	38-39	0.347926176740593
1106	40-41	0.3160198236952212
1106	42-43	0.3286798734312484
1106	44-45	0.2739308082838221
1106	46-47	0.3100533383838311
1106	48-49	0.3332225470857608
1106	50-51	0.29063763834761147
1106	52-53	0.2798358367130689
1106	54-55	0.3147678932183311
1106	56-57	0.23556171708433737
1106	58-59	0.27895998001140754
1106	60-61	0.2127131359694303
1106	62-63	0.26114046220691733
1106	64-65	0.2440856710067223
1106	66-67	0.19850590541270918
1106	68-69	0.2738836966836189
1106	70-71	0.24344993430354123
1106	72-73	0.2482219091189748
1106	74-75	0.23014847964637752
1106	76-77	0.2738277835799039
1106	78-79	0.28037283163444826
1106	80-81	0.2919678141860622
1106	82-83	0.27185368965223944
1106	84-85	0.2985207423432428
1106	86-87	0.24600710595483477
1106	88-89	0.25326194849252204
1106	90-91	0.24237957673305388
1106	92-93	0.28762253798907267
1106	94-95	0.27769499316974233
1106	96-97	0.24826171374965256
1106	98-99	0.2485463895259521
1106	100	0.23821635262001806
1107	1	1.6027659702867645
1107	2	1.2676092296151005
1107	3	1.2242262206928913
1107	4	0.674160417377351
1107	5	1.0194075256702604
1107	6	0.7108410445273066
1107	7	0.4710436416873307
1107	8	0.37828982010567813
1107	9	0.3063670915964636
1107	10-11	0.1730186801280098
1107	12-13	0.10515389943283537
1107	14-15	0.22233757718520764
1107	16-17	0.21342739704822833
1107	18-19	0.22376508495678138
1107	20-21	0.29915612971977623
1107	22-23	0.3114350452460215
1107	24-25	0.3531335287066639
1107	26-27	0.3348903705659225
1107	28-29	0.3433282907374178
1107	30-31	0.3524970273466934
1107	32-33	0.41021572800363515
1107	34-35	0.4186304592889556
1107	36-37	0.42561583320255636
1107	38-39	0.38594880173579327
1107	40-41	0.35258498471802113
1107	42-43	0.36647095161314525
1107	44-45	0.3800687457128191
1107	46-47	0.4041229320499298
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2216	2	-6.293184606639063
2216	3	-5.509583672170418
2216	4	-3.0243983084303494
2216	5	-4.054153650898808
2216	6	-4.444690762896844
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2216	12-13	-0.6602385077102184
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2216	54-55	-1.5463767306612013
2216	56-57	-1.499564284872399
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2216	62-63	-1.4764633537913667
2216	64-65	-1.5745448554971126
2216	66-67	-1.5983231184290005
2216	68-69	-1.5912363068026387
2216	70-71	-1.6286551930455602
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2216	74-75	-1.6020622298371698
2216	76-77	-1.6267522070288898
2216	78-79	-1.60687991365576
2216	80-81	-1.6756100250223938
2216	82-83	-1.6224380428605443
2216	84-85	-1.5774925691074628
2216	86-87	-1.5890561727351091
2216	88-89	-1.5785347182820928
2216	90-91	-1.646725976100523
2216	92-93	-1.6778593188265702
2216	94-95	-1.6950928420037528
2216	96-97	-1.7699557674688116
2216	98-99	-1.839602372940039
2216	100	-1.8528968969406812
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	9693.0
3	11768.0
4	14827.0
5	20003.0
6	14091.0
7	9728.0
8	8606.0
9	9352.0
10	9014.0
11	15054.0
12	10688.0
13	13629.0
14	11914.0
15	12589.0
16	14798.0
17	19059.0
18	24908.0
19	39703.0
20	86603.0
21	125979.0
22	54533.0
23	31344.0
24	30793.0
25	33794.0
26	35317.0
27	38223.0
28	44576.0
29	52005.0
30	61272.0
31	76097.0
32	99723.0
33	138330.0
34	209154.0
35	592493.0
36	649471.0
37	1128632.0
38	1385072.0
39	497443.0
40	4419.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.794772104014804	44.75302235576331	24.821560342622575	16.630645197599307
2	17.072165255282968	42.42509385357619	23.603304127750345	16.899436763390487
3	18.114789863831486	26.074101054494157	19.816422387242397	35.99468669443196
4	33.71043217021922	27.12964468807233	21.61830780661558	17.54161533509287
5	16.71045227569413	41.41363262043228	22.91710664884532	18.95880845502827
6	36.54905520719395	24.33118292828546	22.88106796718515	16.23869389733543
7	18.32059364745353	24.582754397623113	39.53937651569252	17.55727543923084
8	35.48362294734332	25.04977680821486	22.319107650950972	17.147492593490846
9	35.19306705036603	26.547997173276084	22.352324668622604	15.906611107735277
10-11	16.26965982407913	44.54446359122554	22.38922656078794	16.796650023907393
12-13	16.25569981878567	26.087990196816584	31.237655448999302	26.418654535398446
14-15	16.654358640212426	34.99650689439926	22.049968904406718	26.299165560981596
16-17	26.216477500314898	25.63081975077768	31.12762479496177	17.02507795394565
18-19	17.04265968571918	26.031352967218613	31.07200439633872	25.853982950723488
20-21	26.41064701967174	35.25341572807185	21.950354465438977	16.38558278681743
22-23	25.921637402761583	26.164325427297186	31.125425631905212	16.78861153803602
24-25	16.816010346400684	35.177213115480185	21.915545732774085	26.09123080534505
26-27	17.30337106521111	30.348844028284116	35.642131999031236	16.705652907473542
28-29	26.47763579336019	25.925908209983874	30.971426222800822	16.62502977385511
30-31	23.486894293761463	27.692129843911456	31.51379887206639	17.30717699026069
32-33	19.24981320402815	28.926327314376625	32.50405813418395	19.319801347411275
34-35	19.67950639913355	28.8576965387407	32.6906910610597	18.77210600106605
36-37	19.878282146057526	28.332931972214116	33.276761793681956	18.512024088046402
38-39	20.93549843551704	28.195849380107518	32.56824168701197	18.30041049736347
40-41	18.674698581733047	30.31664973297322	32.539761699971564	18.468889985322164
42-43	18.70949065051655	28.336126083733614	33.742684753815126	19.21169851193471
44-45	18.777666721855052	29.31308530804554	32.44055710328121	19.4686908668182
46-47	19.92007973601794	28.193488815733765	33.314129226630776	18.572302221617516
48-49	18.960981598222453	28.389972472543857	33.252438838358	19.396607090875687
50-51	19.821829397954446	29.459265812104977	32.10562067815103	18.613284111789554
52-53	19.997393956991964	28.6761018061065	32.51246817075445	18.81403606614708
54-55	19.612857788290324	30.03662263799931	30.365854306747813	19.984665266962555
56-57	20.024410343940822	30.715877008221614	29.778768191822998	19.480944456014566
58-59	21.967355932209394	30.331339662265965	28.32012497459704	19.3811794309276
60-61	21.118664103121965	31.36652972537803	27.97997012190066	19.534836049599345
62-63	19.945305576942634	31.182438046470327	27.955054304672366	20.917202071914673
64-65	20.623884092503317	31.181040083813656	28.600577361701884	19.594498461981143
66-67	20.70185860354663	30.10971584397205	29.58904039207717	19.599385160404147
68-69	21.430215233401302	30.2258985183991	29.02727754284206	19.316608705357538
70-71	19.202600535326166	32.00197192793161	29.24915699195062	19.5462705447916
72-73	19.28954222439468	30.293372035979715	29.98271916445371	20.4343665751719
74-75	19.43622487329291	31.301401602365324	28.74102056821507	20.521352956126698
76-77	20.752378161521513	30.21686926720097	29.25833180376013	19.772420767517392
78-79	19.980483085710084	30.648961763060406	28.776631440836418	20.59392371039309
80-81	21.070259326745642	31.602935148691717	27.880626018355265	19.44617950620738
82-83	20.844112061506458	31.17406184646047	28.45792422447418	19.52390186755889
84-85	20.353933461657267	31.9183063933684	27.305262051983508	20.422498092990825
86-87	20.275822454067537	32.0313384513411	28.06320808689876	19.629631007692602
88-89	22.18808609179935	30.995695020209247	27.4249476905663	19.391271197425112
90-91	21.026599123069616	32.108877504833515	27.31804438730268	19.546478984794184
92-93	19.96986567928202	32.28320059903635	27.270160466542436	20.476773255139197
94-95	20.761471578732344	32.4427945811878	27.05018546345312	19.745548376626743
96-97	20.830001709736372	31.43881845700279	27.789744948083367	19.941434885177472
98-99	21.88715585317052	31.616011141513432	26.718290075362027	19.778542929954025
100	19.910499954286088	33.29337204959215	26.802049542541933	19.994078453579824
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	311663.0
1	165369.0
2	13790.0
3	6821.5
4	4900.5
5	4507.0
6	4770.5
7	5631.5
8	5252.5
9	4726.5
10	4935.5
11	7188.5
12	66160.0
13	72973.5
14	19947.0
15	17189.5
16	18382.5
17	20052.5
18	21924.5
19	23621.0
20	25708.5
21	28390.5
22	31320.0
23	34831.0
24	38459.0
25	42404.0
26	47170.5
27	52750.5
28	59175.0
29	66125.5
30	72313.5
31	78190.0
32	84443.0
33	90839.5
34	98246.5
35	105410.0
36	112928.5
37	120216.0
38	125534.5
39	129583.0
40	132302.5
41	134969.5
42	138309.0
43	142900.0
44	150254.5
45	155418.0
46	158287.0
47	167996.0
48	175045.0
49	192920.0
50	283262.0
51	286177.5
52	186797.5
53	153410.0
54	147539.5
55	147685.0
56	138757.0
57	120337.5
58	104762.5
59	87924.0
60	73177.5
61	60971.0
62	49672.0
63	39161.0
64	29959.0
65	21736.0
66	16539.5
67	13448.0
68	11235.0
69	9002.0
70	6657.0
71	5233.5
72	4116.5
73	3058.5
74	2325.0
75	1779.5
76	1287.5
77	899.5
78	593.5
79	339.5
80	177.5
81	91.5
82	54.5
83	43.0
84	27.5
85	16.0
86	8.5
87	6.5
88	5.5
89	3.0
90	1.0
91	0.5
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.055875452659372146
2	0.0
3	0.0
4	4.0746208343866823E-4
5	0.009371627919089368
6	0.0031711179537183306
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.1515232792831928E-4
16-17	3.1888336964765336E-4
18-19	0.0
20-21	0.0
22-23	2.6573614137304445E-5
24-25	0.003932894892321058
26-27	0.00705086561776478
28-29	0.0011249496651458883
30-31	0.005394443669872803
32-33	0.0030028183975154025
34-35	0.025386659372504852
36-37	0.02480203986148415
38-39	0.02301274984290565
40-41	0.0023384780440827914
42-43	0.005686753425383152
44-45	0.006971144775352867
46-47	0.0012578177358324105
48-49	0.009105891777716323
50-51	0.009849952973560849
52-53	0.0021967521020171674
54-55	0.011462085564557318
56-57	0.006528251206397792
58-59	0.011869547647995986
60-61	0.007600053643269072
62-63	0.0011160917937667867
64-65	0.013251375583135818
66-67	0.005279291341944483
68-69	0.0022498993302917767
70-71	0.008406119938767307
72-73	0.005208428370911671
74-75	0.00847698290980012
76-77	0.0074583277012034484
78-79	0.015492417042048493
80-81	0.00860099310910754
82-83	0.011896121262133292
84-85	0.006864850318803648
86-87	0.012471882901774888
88-89	0.01977962678953361
90-91	0.018309220140602763
92-93	0.017113407504424062
94-95	0.005731042782278659
96-97	0.009779090002528036
98-99	0.016643940321331683
100	0.01596188422514087
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5644697.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.12905105452608
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.27163001128254	32.876926595297455
2	16.733303156361888	15.437827912213601
3	6.56202307008331	9.08099691662553
4	2.5171126360878393	4.644480692003547
5	1.0843548526677278	2.501013017996636
6	0.5309462389943277	1.4695227699466769
7	0.28811529155500754	0.9303339495611428
8	0.19726643205601313	0.7279770652524824
9	0.138940211078559	0.576826208133253
>10	0.5414040956742399	4.196113858697788
>50	0.05770500388866133	1.9388147515066925
>100	0.07110884887393162	5.133793097425625
>500	0.002389807280488947	0.7387115106700628
>1k	0.0029294390913791107	3.662154972773226
>5k	6.167240192375907E-4	1.8032331316091266
>10k+	1.5418100480939768E-4	14.281273550287176
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	407488	7.21895258505461	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	300844	5.3296749143488125	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	83871	1.485837060873241	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	10993	0.1947491601409252	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9405	0.1666165606408989	No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT	8521	0.15095584404264747	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8134	0.1440998515952229	No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7292	0.129183196192816	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6871	0.12172486849161257	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6598	0.11688847071862317	No Hit
TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG	6054	0.10725110665816076	No Hit
AGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTC	5883	0.10422171464650803	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.8974634068046523E-4	0.0	0.0	0.0	0.0
2	3.8974634068046523E-4	0.0	0.0	0.0	1.7715742758202964E-5
3	3.8974634068046523E-4	0.0	0.0	0.0	1.7715742758202964E-5
4	3.8974634068046523E-4	0.0	0.0	0.0	1.7715742758202964E-5
5	3.8974634068046523E-4	0.0	0.0	0.0	1.7715742758202964E-5
6	4.074620834386682E-4	0.0	0.0	1.7715742758202964E-5	1.7715742758202964E-5
7	4.074620834386682E-4	0.0	0.0	3.543148551640593E-5	1.7715742758202964E-5
8	4.2517782619687117E-4	0.0	0.0	3.543148551640593E-5	1.7715742758202964E-5
9	4.2517782619687117E-4	0.0	0.0	3.543148551640593E-5	1.7715742758202964E-5
10-11	4.428935689550741E-4	0.0	0.0	3.543148551640593E-5	1.7715742758202964E-5
12-13	4.871829258505815E-4	0.0	0.0	3.543148551640593E-5	1.7715742758202964E-5
14-15	6.111931251580023E-4	0.0	0.0	3.543148551640593E-5	1.7715742758202964E-5
16-17	8.680713951519452E-4	0.0	0.0	3.543148551640593E-5	1.7715742758202964E-5
18-19	0.0010363709513548734	0.0	0.0	3.543148551640593E-5	1.7715742758202964E-5
20-21	0.001213528378936903	0.0	0.0	3.543148551640593E-5	1.7715742758202964E-5
22-23	0.001452690906172643	0.0	0.0	3.543148551640593E-5	1.7715742758202964E-5
24-25	0.001594416848238267	0.0	0.0	3.543148551640593E-5	1.7715742758202964E-5
26-27	0.0017184270475456877	0.0	0.0	3.543148551640593E-5	1.7715742758202964E-5
28-29	0.0018247215040949054	0.0	0.0	3.543148551640593E-5	1.7715742758202964E-5
30-31	0.0019398738320232246	0.0	0.0	3.543148551640593E-5	1.7715742758202964E-5
32-33	0.0021701784878798633	1.7715742758202964E-5	0.0	3.543148551640593E-5	1.7715742758202964E-5
34-35	0.0024181988864947048	1.7715742758202964E-5	0.0	3.543148551640593E-5	1.7715742758202964E-5
36-37	0.002692792899246851	1.7715742758202964E-5	0.0	3.543148551640593E-5	1.7715742758202964E-5
38-39	0.003303986024404853	1.7715742758202964E-5	0.0	3.543148551640593E-5	1.7715742758202964E-5
40-41	0.003977184249216566	1.7715742758202964E-5	0.0	3.543148551640593E-5	1.7715742758202964E-5
42-43	0.004570661631616365	1.7715742758202964E-5	0.0	3.543148551640593E-5	1.7715742758202964E-5
44-45	0.005181854756774367	1.7715742758202964E-5	0.0	3.543148551640593E-5	1.7715742758202964E-5
46-47	0.005846195110206978	3.543148551640593E-5	0.0	3.543148551640593E-5	1.7715742758202964E-5
48-49	0.006537109077776894	3.543148551640593E-5	0.0	3.543148551640593E-5	1.7715742758202964E-5
50-51	0.007272312402242317	3.543148551640593E-5	0.0	3.543148551640593E-5	1.7715742758202964E-5
52-53	0.008361830581871798	3.543148551640593E-5	0.0	3.543148551640593E-5	1.7715742758202964E-5
54-55	0.009708227031495225	3.543148551640593E-5	0.0	3.543148551640593E-5	1.7715742758202964E-5
56-57	0.011019191995602244	3.543148551640593E-5	0.0	3.543148551640593E-5	1.7715742758202964E-5
58-59	0.012675613943494222	3.543148551640593E-5	0.0	3.543148551640593E-5	1.7715742758202964E-5
60-61	0.014836934559994982	3.543148551640593E-5	0.0	3.543148551640593E-5	1.7715742758202964E-5
62-63	0.017884042314405893	3.543148551640593E-5	0.0	3.543148551640593E-5	2.6573614137304445E-5
64-65	0.02121460195294805	3.543148551640593E-5	0.0	3.543148551640593E-5	3.543148551640593E-5
66-67	0.025439806600779456	3.543148551640593E-5	0.0	3.543148551640593E-5	3.543148551640593E-5
68-69	0.030515366901004606	5.3147228274608896E-5	0.0	3.543148551640593E-5	3.543148551640593E-5
70-71	0.037468795933599267	5.3147228274608896E-5	0.0	3.543148551640593E-5	3.543148551640593E-5
72-73	0.04653039835442009	5.3147228274608896E-5	0.0	3.543148551640593E-5	3.543148551640593E-5
74-75	0.05710669678106725	5.3147228274608896E-5	0.0	3.543148551640593E-5	3.543148551640593E-5
76-77	0.07083639741867455	6.200509965371037E-5	0.0	4.428935689550741E-5	3.543148551640593E-5
78-79	0.08827754616412538	7.086297103281186E-5	0.0	5.3147228274608896E-5	3.543148551640593E-5
80-81	0.11076768159566402	7.086297103281186E-5	1.7715742758202964E-5	5.3147228274608896E-5	3.543148551640593E-5
82-83	0.13885599173879484	7.086297103281186E-5	1.7715742758202964E-5	5.3147228274608896E-5	3.543148551640593E-5
84-85	0.17500496483690797	7.086297103281186E-5	1.7715742758202964E-5	5.3147228274608896E-5	3.543148551640593E-5
86-87	0.21925003237551988	7.972084241191334E-5	1.7715742758202964E-5	5.3147228274608896E-5	3.543148551640593E-5
88	0.2592167480380258	8.857871379101482E-5	1.7715742758202964E-5	5.3147228274608896E-5	3.543148551640593E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	2720	0.0	72.96184	1
GTGGAAT	163455	0.0	59.28047	6
AACGAGT	157325	0.0	58.60021	1
TGGAATC	166035	0.0	58.518578	7
GAATCAC	166605	0.0	58.35221	9
AGTGGAA	164190	0.0	58.170467	5
GGAATCA	167235	0.0	58.16892	8
ACGAGTG	163150	0.0	57.827374	2
CGAGTGG	166835	0.0	56.933224	3
GAGTGGA	170810	0.0	56.00728	4
CGTGTGG	2605	0.0	51.05818	3
AACGTGT	2185	0.0	48.64175	1
ACGTGTG	2610	0.0	46.998787	2
AGTACTT	59080	0.0	41.056297	20-21
ATCTGCG	2985	0.0	40.93769	94
GAGTACT	58650	0.0	40.49995	20-21
TCGAGTG	1515	0.0	40.01877	2
GTACTTT	58710	0.0	39.771236	22-23
TACTTTT	61150	0.0	39.08731	22-23
ATCGAGT	1340	0.0	38.60469	1
>>END_MODULE