FastQCFastQC Report
Thu 26 May 2016
SRR2132703_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132703_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3987524
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2828307.092872669857285No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA863452.165378816528753No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG224020.5618022612528476No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA97750.2451395903824027No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64060.16065107068948048No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT54300.13617472897968766No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42880.10753540292171282No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC41430.10389906117179483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAGT818500.075.078741
TACGAGT17200.074.359041
ACGAGTG852300.073.694542
GTGGAAT880950.073.0010156
CGAGTGG871950.072.427213
TGGAATC893350.072.1928567
GAATCAC898000.071.934149
GGAATCA898100.071.889518
AGTGGAA887000.071.680415
GAGTGGA900200.070.6972054
CGTGTGG16700.048.1178253
ATCGAGT7950.045.5425031
AACGTGT14800.044.1617621
ACGTGTG17700.042.213392
GAGTACT407200.041.5968920-21
AGTACTT416200.041.57242220-21
GTACTTT413800.040.20789722-23
TCGAGTG10300.040.148732
CATGGGG202850.039.3398424-25
TACTTTT450850.037.8834822-23