##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2132703_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3987524 Sequences flagged as poor quality 0 Sequence length 100 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.804499233108064 33.0 31.0 34.0 25.0 34.0 2 31.557265611442087 34.0 31.0 34.0 28.0 34.0 3 31.763949006952686 34.0 31.0 34.0 28.0 34.0 4 35.72205834999363 37.0 35.0 37.0 33.0 37.0 5 35.37663020962382 37.0 35.0 37.0 33.0 37.0 6 35.72344091220517 37.0 35.0 37.0 35.0 37.0 7 35.915800632171745 37.0 35.0 37.0 35.0 37.0 8 36.054619106994714 37.0 37.0 37.0 35.0 37.0 9 37.96860357454902 39.0 39.0 39.0 37.0 39.0 10-11 38.04078934697321 39.0 39.0 39.0 37.0 39.0 12-13 38.01775161729434 39.0 39.0 39.0 37.0 39.0 14-15 39.52439471210706 41.0 40.0 41.0 37.0 41.0 16-17 39.45378034088321 41.0 40.0 41.0 37.0 41.0 18-19 39.365328459465076 41.0 40.0 41.0 36.5 41.0 20-21 39.23958927896108 41.0 39.5 41.0 36.0 41.0 22-23 39.144248536184364 41.0 39.0 41.0 36.0 41.0 24-25 39.09487892737448 41.0 39.0 41.0 35.0 41.0 26-27 39.00658842429537 41.0 39.0 41.0 35.0 41.0 28-29 38.83246633750668 41.0 39.0 41.0 35.0 41.0 30-31 38.72858508187035 41.0 39.0 41.0 35.0 41.0 32-33 38.5794252272839 41.0 39.0 41.0 35.0 41.0 34-35 38.49032858485617 41.0 39.0 41.0 35.0 41.0 36-37 38.36598764045056 40.5 38.5 41.0 35.0 41.0 38-39 37.95240680683051 40.0 37.0 41.0 34.5 41.0 40-41 37.745717894111735 40.0 36.0 41.0 34.0 41.0 42-43 37.638393524402616 40.0 36.0 41.0 33.5 41.0 44-45 37.483522105446895 40.0 35.5 41.0 33.0 41.0 46-47 37.38843364955296 40.0 35.0 41.0 33.0 41.0 48-49 37.240130968490725 40.0 35.0 41.0 33.0 41.0 50-51 37.00375257929482 40.0 35.0 41.0 33.0 41.0 52-53 36.56594994788746 40.0 35.0 41.0 31.5 41.0 54-55 35.746977196877054 39.5 35.0 41.0 28.5 41.0 56-57 35.11493899472455 39.0 35.0 41.0 23.5 41.0 58-59 34.80533383623522 39.0 35.0 41.0 22.0 41.0 60-61 34.571164336565744 39.0 35.0 41.0 22.0 41.0 62-63 34.29951305622236 38.5 35.0 41.0 20.0 41.0 64-65 34.01468931597653 37.5 35.0 40.0 19.5 41.0 66-67 33.7193552941625 37.0 34.5 40.0 18.5 41.0 68-69 33.419313087519974 36.5 34.0 40.0 17.5 41.0 70-71 33.084746073001696 36.0 34.0 39.0 14.5 41.0 72-73 32.750472348254206 35.5 34.0 39.0 12.0 41.0 74-75 32.395946331608286 35.0 34.0 38.5 7.5 40.0 76-77 32.02087636337737 35.0 33.5 37.0 7.0 39.5 78-79 31.684876630209622 35.0 33.0 37.0 2.0 39.0 80-81 31.361973871505228 35.0 33.0 36.5 2.0 39.0 82-83 31.043348704609677 35.0 33.0 36.0 2.0 38.0 84-85 30.72051704265604 35.0 33.0 36.0 2.0 37.0 86-87 30.5991305632267 35.0 33.0 35.0 2.0 37.0 88-89 30.666225833374295 35.0 33.0 35.0 2.0 36.5 90-91 30.550225026858772 35.0 33.0 35.0 2.0 36.0 92-93 30.411896329652187 35.0 33.0 35.0 2.0 36.0 94-95 30.26530398312336 35.0 33.0 35.0 2.0 36.0 96-97 30.11349298461903 35.0 33.0 35.0 2.0 36.0 98-99 29.986715816632074 35.0 33.0 35.0 2.0 35.0 100 29.898109202602917 35.0 33.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.9075041768066363 1101 2 1.4069324522340345 1101 3 1.3328973130027713 1101 4 0.6725099786912878 1101 5 0.9538677630176409 1101 6 0.624768029429319 1101 7 0.41464350197219346 1101 8 0.3075283388643939 1101 9 0.3544943273052823 1101 10-11 0.22456299954797743 1101 12-13 0.2684955735533805 1101 14-15 0.3843567081824233 1101 16-17 0.3620394318139617 1101 18-19 0.32326575195957474 1101 20-21 0.43688923255370327 1101 22-23 0.43459606057827926 1101 24-25 0.4006584819172261 1101 26-27 0.4309774058900828 1101 28-29 0.4507372475966491 1101 30-31 0.4824305830763862 1101 32-33 0.4167846724513993 1101 34-35 0.45720220636879816 1101 36-37 0.4811312045239262 1101 38-39 0.46734552642951144 1101 40-41 0.4758889036402252 1101 42-43 0.4624091358491924 1101 44-45 0.4794510306559374 1101 46-47 0.46434114542476834 1101 48-49 0.4172295525829952 1101 50-51 0.35643188305733986 1101 52-53 0.32913857778945754 1101 54-55 0.2244742931316992 1101 56-57 0.31905015469301645 1101 58-59 0.2600718148007104 1101 60-61 0.24263341175047515 1101 62-63 0.2540146894721218 1101 64-65 0.2955097045612405 1101 66-67 0.2961200469452834 1101 68-69 0.31218846365946007 1101 70-71 0.29659299318503685 1101 72-73 0.34863521496875194 1101 74-75 0.3052253507446636 1101 76-77 0.3643321815214051 1101 78-79 0.2955464848742615 1101 80-81 0.3338195956365624 1101 82-83 0.344325701951373 1101 84-85 0.39554097851019776 1101 86-87 0.3955990113065937 1101 88-89 0.4017338202657008 1101 90-91 0.36372003225559624 1101 92-93 0.33548159705300407 1101 94-95 0.31490065956597846 1101 96-97 0.3192680002133308 1101 98-99 0.3597375837730681 1101 100 0.37862501279790806 1102 1 1.3472208569084039 1102 2 1.2019440652184237 1102 3 1.124257382523421 1102 4 0.5851498079800805 1102 5 0.9288687031326575 1102 6 0.5780236990065788 1102 7 0.338916929809308 1102 8 0.2990317160993925 1102 9 0.29285437016085325 1102 10-11 0.259588659559995 1102 12-13 0.24827537468657823 1102 14-15 0.3697606362906356 1102 16-17 0.36663681644817814 1102 18-19 0.3573811071487043 1102 20-21 0.4579458432031842 1102 22-23 0.469062232505145 1102 24-25 0.4502280117981883 1102 26-27 0.3386441976775103 1102 28-29 0.424283163155863 1102 30-31 0.44504150810170273 1102 32-33 0.42641466369293823 1102 34-35 0.398860053047315 1102 36-37 0.429148758351495 1102 38-39 0.441080627315813 1102 40-41 0.44398023769419837 1102 42-43 0.40398517542837453 1102 44-45 0.4654108060402393 1102 46-47 0.42818418518923806 1102 48-49 0.4530389779368349 1102 50-51 0.3668195728152881 1102 52-53 0.34898001286305913 1102 54-55 0.3603398518984591 1102 56-57 0.3434568224673029 1102 58-59 0.37387352369050575 1102 60-61 0.4086876020311294 1102 62-63 0.4378615819988525 1102 64-65 0.324048434223144 1102 66-67 0.3909269883443258 1102 68-69 0.4123662137693671 1102 70-71 0.29882682611258105 1102 72-73 0.3029701878626696 1102 74-75 0.2639445756523102 1102 76-77 0.27097164980602173 1102 78-79 0.30658199934280006 1102 80-81 0.3386847250271572 1102 82-83 0.2597937390664349 1102 84-85 0.27857410747962064 1102 86-87 0.21614763508076962 1102 88-89 0.21893819853516305 1102 90-91 0.2794642922551702 1102 92-93 0.34095800631959605 1102 94-95 0.3305775218654503 1102 96-97 0.2958074470975163 1102 98-99 0.38461234336001837 1102 100 0.43467391792286847 1103 1 1.868788546441504 1103 2 1.3688367700931856 1103 3 1.244062456391152 1103 4 0.6576886444795562 1103 5 0.9448221502488252 1103 6 0.6082203669863304 1103 7 0.38201412221913955 1103 8 0.2981448477535835 1103 9 0.3254391110409429 1103 10-11 0.2583055209116196 1103 12-13 0.23895059516427608 1103 14-15 0.3653307161744408 1103 16-17 0.3880914868772294 1103 18-19 0.3448547449569759 1103 20-21 0.4248125543539558 1103 22-23 0.4358672420585137 1103 24-25 0.39275709395175795 1103 26-27 0.35993051184784264 1103 28-29 0.4157659309247208 1103 30-31 0.4265552137616382 1103 32-33 0.3966526536545416 1103 34-35 0.3979026398784171 1103 36-37 0.4498669622655669 1103 38-39 0.49322102290277314 1103 40-41 0.4635085587944019 1103 42-43 0.44938442027049774 1103 44-45 0.450044160543726 1103 46-47 0.41490514390208943 1103 48-49 0.44794903161253785 1103 50-51 0.49234440486098663 1103 52-53 0.4893482662187978 1103 54-55 0.4285366645855433 1103 56-57 0.4939383763024523 1103 58-59 0.44042911408300967 1103 60-61 0.46285081849988785 1103 62-63 0.41988384012993407 1103 64-65 0.3692677998154892 1103 66-67 0.45016279965668105 1103 68-69 0.40956806977485627 1103 70-71 0.36906401214457674 1103 72-73 0.3787732465468494 1103 74-75 0.37615901315616185 1103 76-77 0.42716519049960056 1103 78-79 0.3998665743454133 1103 80-81 0.4397087303652505 1103 82-83 0.45504908050873993 1103 84-85 0.47938259268865835 1103 86-87 0.4345381278092262 1103 88-89 0.502156904905231 1103 90-91 0.5682293759844406 1103 92-93 0.5689618892996045 1103 94-95 0.5493384251607623 1103 96-97 0.5253450267870399 1103 98-99 0.4988289675524058 1103 100 0.5593040433090906 1104 1 1.9026880615097248 1104 2 1.3816603447008795 1104 3 1.2674142430170612 1104 4 0.6975894599579249 1104 5 0.962191510901043 1104 6 0.6004863419498392 1104 7 0.35311248088787295 1104 8 0.24689715762509223 1104 9 0.2646632972317349 1104 10-11 0.2220863761213181 1104 12-13 0.2441181462331059 1104 14-15 0.35901098989742763 1104 16-17 0.33746158144971616 1104 18-19 0.35661589422783635 1104 20-21 0.35814522002015536 1104 22-23 0.3938730333005651 1104 24-25 0.4060718532988332 1104 26-27 0.3896476058728382 1104 28-29 0.41022840238194647 1104 30-31 0.40903768236329796 1104 32-33 0.35391868254501446 1104 34-35 0.34983221075333404 1104 36-37 0.37841290392788807 1104 38-39 0.36990656143795775 1104 40-41 0.3451524246282318 1104 42-43 0.33129875549486343 1104 44-45 0.41905432603272885 1104 46-47 0.4215515134876995 1104 48-49 0.3873156106607283 1104 50-51 0.32273570146030295 1104 52-53 0.28411528377022677 1104 54-55 0.25988381657431603 1104 56-57 0.2276127591469006 1104 58-59 0.14696809197706528 1104 60-61 0.15008875914735853 1104 62-63 0.15578572747086383 1104 64-65 0.14954772439991615 1104 66-67 0.14452718824306743 1104 68-69 0.175523452661416 1104 70-71 0.1699541793592445 1104 72-73 0.1662036936064908 1104 74-75 0.18587824371600448 1104 76-77 0.27404985562344564 1104 78-79 0.258398050742354 1104 80-81 0.21557710851958944 1104 82-83 0.255337641110831 1104 84-85 0.24667825316039682 1104 86-87 0.22338287362834564 1104 88-89 0.16148231457038165 1104 90-91 0.14439909029480447 1104 92-93 0.11374457134932214 1104 94-95 0.17080486102003078 1104 96-97 0.19656678831893615 1104 98-99 0.23460836365261173 1104 100 0.2755050635044789 1105 1 1.6119788003416637 1105 2 1.2777814546534607 1105 3 1.2169574367200262 1105 4 0.6327044778345794 1105 5 1.0138146232173 1105 6 0.665882103240655 1105 7 0.4130224913146776 1105 8 0.30347039225003414 1105 9 0.24069519291202113 1105 10-11 0.2214123364756091 1105 12-13 0.25268714701933703 1105 14-15 0.27926520410511557 1105 16-17 0.2895407173072826 1105 18-19 0.3290904136655257 1105 20-21 0.3513299026586836 1105 22-23 0.38803156344933853 1105 24-25 0.38507766159474954 1105 26-27 0.3870747002537982 1105 28-29 0.47240430424078284 1105 30-31 0.42964565245657127 1105 32-33 0.45695847088786934 1105 34-35 0.3924907200286185 1105 36-37 0.4030106296125169 1105 38-39 0.44401035584007786 1105 40-41 0.43288983567985184 1105 42-43 0.47224696837658797 1105 44-45 0.4678984691811081 1105 46-47 0.46591189521405596 1105 48-49 0.3907712907835048 1105 50-51 0.41089716731575265 1105 52-53 0.3825046334219806 1105 54-55 0.34914157529868817 1105 56-57 0.2961333309611405 1105 58-59 0.21691477016059224 1105 60-61 0.22683354649127097 1105 62-63 0.29393186125400206 1105 64-65 0.20339777673272863 1105 66-67 0.2707856233677788 1105 68-69 0.23674470316213103 1105 70-71 0.21999941766984676 1105 72-73 0.21352711634129662 1105 74-75 0.30079249841825373 1105 76-77 0.3639758143873806 1105 78-79 0.30364468588674143 1105 80-81 0.29511428370081916 1105 82-83 0.34187637981554175 1105 84-85 0.3629717731358255 1105 86-87 0.3172372721190939 1105 88-89 0.22624404372994533 1105 90-91 0.2018452217918032 1105 92-93 0.23085520132106296 1105 94-95 0.24586200218741538 1105 96-97 0.2585376206044536 1105 98-99 0.16634069648332428 1105 100 0.2244543568375903 1106 1 1.6615684850472583 1106 2 1.3354063108838297 1106 3 1.2010163126894078 1106 4 0.6720225079954218 1106 5 1.0224705011138298 1106 6 0.6660837759263671 1106 7 0.3851963312536313 1106 8 0.2975823641825599 1106 9 0.24548958976458124 1106 10-11 0.157691132916014 1106 12-13 0.14702964137034513 1106 14-15 0.21222825728587225 1106 16-17 0.24726286503994999 1106 18-19 0.273106902443466 1106 20-21 0.3086012209226041 1106 22-23 0.3353024194468972 1106 24-25 0.34713744868396645 1106 26-27 0.3132789041472819 1106 28-29 0.41074868885406346 1106 30-31 0.4035830505723439 1106 32-33 0.4146554694138871 1106 34-35 0.4199403006596114 1106 36-37 0.45845235506843807 1106 38-39 0.45199654629095676 1106 40-41 0.43324360118672445 1106 42-43 0.4845346312296783 1106 44-45 0.4391464590409413 1106 46-47 0.4662660199953166 1106 48-49 0.49001946123860307 1106 50-51 0.4280280284581224 1106 52-53 0.40804934128640724 1106 54-55 0.42450404722139723 1106 56-57 0.27517793415695735 1106 58-59 0.31120603198490926 1106 60-61 0.3118145121345819 1106 62-63 0.33300630338471393 1106 64-65 0.43386319034615894 1106 66-67 0.3583259399412029 1106 68-69 0.4063229294101447 1106 70-71 0.4095199468474746 1106 72-73 0.3932797535350119 1106 74-75 0.3707769921359798 1106 76-77 0.4027043950475857 1106 78-79 0.37157921986715436 1106 80-81 0.3603918638776875 1106 82-83 0.39691835264385844 1106 84-85 0.3516821146333129 1106 86-87 0.3425436613109696 1106 88-89 0.32562227151086276 1106 90-91 0.28197424680256944 1106 92-93 0.2980107685650104 1106 94-95 0.2286125206203451 1106 96-97 0.24262853187277855 1106 98-99 0.28872074794099944 1106 100 0.3094789144390937 1107 1 1.6884355497394345 1107 2 1.3032741861655062 1107 3 1.2247432002588603 1107 4 0.678436588936421 1107 5 0.996612558639626 1107 6 0.6726212968940288 1107 7 0.44264223366194244 1107 8 0.3651836090066496 1107 9 0.3353124396107603 1107 10-11 0.20910253246532307 1107 12-13 0.12004325131906768 1107 14-15 0.22024688712900797 1107 16-17 0.19214620735696997 1107 18-19 0.2004291865228609 1107 20-21 0.2956863081329857 1107 22-23 0.2823665355682934 1107 24-25 0.23893163575595366 1107 26-27 0.27540356074398176 1107 28-29 0.24811131039105305 1107 30-31 0.24940662871468078 1107 32-33 0.2861917765546167 1107 34-35 0.27226573931745435 1107 36-37 0.26817349719043904 1107 38-39 0.33852395889198306 1107 40-41 0.2646118378733817 1107 42-43 0.30641513031142154 1107 44-45 0.3355180423238693 1107 46-47 0.27149274503540255 1107 48-49 0.29427718206937925 1107 50-51 0.27255051443053446 1107 52-53 0.209165170549646 1107 54-55 0.25378700467984316 1107 56-57 0.2841458520940279 1107 58-59 0.20405151661731225 1107 60-61 0.18027939138966076 1107 62-63 0.13957252415857369 1107 64-65 0.1735643488993901 1107 66-67 0.19891000328513542 1107 68-69 0.1781651277947276 1107 70-71 0.17678269168733607 1107 72-73 0.16259125992851864 1107 74-75 0.16996012775954483 1107 76-77 0.19338268427853933 1107 78-79 0.1738793876084337 1107 80-81 0.17771794030900878 1107 82-83 0.1661365932394432 1107 84-85 0.20492941026805767 1107 86-87 0.18417319310295 1107 88-89 0.15583788694854306 1107 90-91 0.15662467150484005 1107 92-93 0.1491554690789343 1107 94-95 0.17792804567250187 1107 96-97 0.16547885809734098 1107 98-99 0.13607035341295415 1107 100 0.1685539508881888 1108 1 0.8350442769928215 1108 2 0.9483206765616323 1108 3 1.0094073771951706 1108 4 0.5537429219582037 1108 5 0.9251215305654839 1108 6 0.5949573198846352 1108 7 0.3840432092659327 1108 8 0.30408894508578044 1108 9 0.2531029976104193 1108 10-11 0.20319065394998148 1108 12-13 0.14690026548760926 1108 14-15 0.14087745795812623 1108 16-17 0.1512017725970054 1108 18-19 0.15739093580793195 1108 20-21 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-0.9447335778234063 2215 50-51 -0.9012596050863237 2215 52-53 -0.9205299842600354 2215 54-55 -1.0770220188300712 2215 56-57 -0.9094396588096458 2215 58-59 -0.9020370331029213 2215 60-61 -0.9195549405099115 2215 62-63 -0.9606552109725399 2215 64-65 -0.8445080996080918 2215 66-67 -0.8159669245254477 2215 68-69 -0.9036769278759209 2215 70-71 -0.9177879513068703 2215 72-73 -0.9212146797085481 2215 74-75 -0.8705854271621689 2215 76-77 -0.7797620135999708 2215 78-79 -0.8363902049457153 2215 80-81 -0.8444343706146675 2215 82-83 -0.8756882313602077 2215 84-85 -0.8887068586826423 2215 86-87 -0.8765276702850002 2215 88-89 -0.8141946183421389 2215 90-91 -0.7805264319295269 2215 92-93 -0.7726353680534501 2215 94-95 -0.7926227722762178 2215 96-97 -0.8787129339444455 2215 98-99 -0.8528617943871382 2215 100 -0.8573855575851823 2216 1 -6.262655910999392 2216 2 -6.193638762499855 2216 3 -5.542391325212023 2216 4 -3.031305056044772 2216 5 -4.222085267777633 2216 6 -4.472322057397296 2216 7 -2.157523067763954 2216 8 -1.352137413007874 2216 9 -1.0321017794442895 2216 10-11 -0.8883363859279143 2216 12-13 -0.790898611117008 2216 14-15 -1.0623687104284087 2216 16-17 -0.9385810852507035 2216 18-19 -0.9149783393833388 2216 20-21 -0.9522756744419283 2216 22-23 -0.9429454337304009 2216 24-25 -1.47079013880235 2216 26-27 -1.3276355600567769 2216 28-29 -2.0801337675258793 2216 30-31 -1.5669662923560495 2216 32-33 -1.3647064836599583 2216 34-35 -1.303044627173648 2216 36-37 -1.3014516229469493 2216 38-39 -1.3330428345648144 2216 40-41 -1.2978651995292765 2216 42-43 -1.3764025307839134 2216 44-45 -1.33071776916956 2216 46-47 -1.3343183180549119 2216 48-49 -1.3933617462188153 2216 50-51 -1.3807742793781514 2216 52-53 -1.3972014593183175 2216 54-55 -1.338231057424771 2216 56-57 -1.301810999154597 2216 58-59 -1.3210201266864132 2216 60-61 -1.3313534055373992 2216 62-63 -1.4072025976913878 2216 64-65 -1.4606878030344745 2216 66-67 -1.3853243787088516 2216 68-69 -1.4855551540300809 2216 70-71 -1.408959390662723 2216 72-73 -1.4095936683520591 2216 74-75 -1.4203510906347745 2216 76-77 -1.42012233097228 2216 78-79 -1.370834040640382 2216 80-81 -1.3887580654417775 2216 82-83 -1.3490582422949338 2216 84-85 -1.3095470527586102 2216 86-87 -1.3961516557427807 2216 88-89 -1.285671528923963 2216 90-91 -1.3300671219153628 2216 92-93 -1.3379422225561086 2216 94-95 -1.3132748460558687 2216 96-97 -1.3845512805403928 2216 98-99 -1.485374121672681 2216 100 -1.4979951765550688 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5241.0 3 3901.0 4 4967.0 5 6554.0 6 4869.0 7 3870.0 8 3729.0 9 4097.0 10 4505.0 11 9815.0 12 6321.0 13 9450.0 14 7876.0 15 7905.0 16 9559.0 17 12219.0 18 16640.0 19 27218.0 20 60496.0 21 89826.0 22 42053.0 23 25063.0 24 24128.0 25 25576.0 26 26077.0 27 27309.0 28 31678.0 29 36825.0 30 43322.0 31 53552.0 32 68184.0 33 91842.0 34 134508.0 35 288493.0 36 407232.0 37 764337.0 38 1138306.0 39 456893.0 40 3088.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 13.72462473464185 42.638601016767126 26.268223769064384 17.368550479526647 2 17.46537450307509 40.00419809385473 25.39553366951522 17.134893733554957 3 18.51833832682595 25.31661886338963 20.890713357640823 35.2743294521436 4 33.04619522148147 26.021313540874612 22.89678375889527 18.03570747874865 5 17.426719329363234 39.15269005120467 23.972721271609494 19.447869347822596 6 35.77854001348751 23.244761677617433 24.177501440164036 16.799196868731023 7 18.787548363345273 23.4981657790649 39.57889657842812 18.135389279161707 8 34.65862525216149 23.62019639254836 23.902953311378187 17.818225043911962 9 34.41784927187899 25.296600095698484 23.56560110986166 16.719949522560867 10-11 16.88055043681242 42.144849786484045 23.698628522361247 17.27597125434229 12-13 16.85224966671047 24.89885954291435 31.989738494363923 26.25915229601126 14-15 17.185349735681477 33.24304326111591 23.490649635857487 26.080957367345132 16-17 26.029118901932108 24.548546926367386 31.965876976451234 17.45645719524927 18-19 17.45474133823395 24.804690830700956 31.97620879523233 25.76435903583276 20-21 26.187829841274933 33.478895675612236 23.305063493034776 17.028210990078055 22-23 25.827064298696744 24.923458112148584 31.968408955686144 17.28106863346853 24-25 17.31540559119441 33.35805718897411 23.327415491437105 25.999121728394382 26-27 17.689798733462915 28.621380650824502 36.48677660041382 17.202044015298764 28-29 25.81416164009514 24.669201770744202 32.33096575377507 17.18567083538559 30-31 23.51748193696616 25.958113875424566 32.71325841355288 17.811145774056396 32-33 19.910979820111326 27.327171355946422 33.772432008191785 18.989416815750474 34-35 19.736915873608545 26.976877950803953 34.003445862989004 19.282760312598494 36-37 19.609719559776227 27.168922854481025 34.34908609468212 18.87227149106062 38-39 20.12979284290699 26.952069282046963 33.9471720754752 18.970965799570845 40-41 19.342894718098986 27.962661645551968 33.84553902916868 18.848904607180366 42-43 19.389960455523276 27.1045756100803 34.26102865798949 19.244435276406925 44-45 19.408184519424236 27.521514036400024 33.763879492554025 19.30642195162171 46-47 19.920250283700838 26.979780437494906 34.03183719020182 19.068132088602436 48-49 19.395156628204237 27.42259506152342 33.91492636025302 19.267321950019326 50-51 19.82439705852846 27.791212704909814 33.32992234996689 19.054467886594836 52-53 19.9237305329408 27.582552318642456 33.200930120345355 19.292787028071388 54-55 20.152471144388855 28.351704687890127 31.801686936668332 19.694137231052686 56-57 20.45289303041817 28.97791839247803 30.72473418429471 19.844454392809087 58-59 21.306180781000187 29.264087277028494 29.748144514141295 19.681587427830024 60-61 21.020380421492828 29.532888795144675 29.49555691713176 19.95117386623074 62-63 20.289157236849448 29.745695563205327 29.54264775198499 20.422499447960238 64-65 20.66415726581904 29.582500158326763 29.867826935364434 19.885515640489764 66-67 20.59461768817833 29.03064892383985 30.328393777094607 20.046339610887213 68-69 20.850628793397064 29.177402986905005 30.076200548296857 19.895767671401078 70-71 19.81648805964759 29.89235173376887 30.20741371284566 20.083746493737888 72-73 19.85863165027886 29.139804389621688 30.520871945374754 20.4806920147247 74-75 20.085849557616452 29.45920170946709 29.92524216509926 20.5297065678172 76-77 20.691561253288306 29.004932987463256 30.193725532881277 20.109780226367167 78-79 20.42930962523602 29.436552279745648 29.656721351935424 20.47741674308291 80-81 20.685929029742763 29.739023985595924 29.491595053227126 20.083451931434183 82-83 20.720496230765065 29.649103489928308 29.524950489288234 20.1054497900184 84-85 20.58815821005163 30.025768428740307 29.004574212736195 20.38149914847187 86-87 20.634660065881608 30.35670549828123 28.971340735974326 20.03729369986284 88-89 21.38596449863248 30.01454057112815 28.506893790872624 20.092601139366746 90-91 20.96509108138475 30.552079193417654 28.4959553872967 19.986874337900893 92-93 20.42507968112001 30.615045190049532 28.4922679151777 20.467607213652755 94-95 20.671437822242353 30.832765278908454 28.382720210749447 20.11307668809975 96-97 20.495398985154534 30.15373668568671 28.893600740085017 20.457263589073737 98-99 21.05234312763494 30.354233261295754 28.084560380133684 20.508863230935624 100 20.538153102016675 31.0806655265876 27.94468727002567 20.43649410137005 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 89338.0 1 48216.5 2 5274.0 3 2907.0 4 2225.0 5 1984.5 6 2032.0 7 2402.5 8 2239.5 9 2016.0 10 2184.0 11 3202.0 12 31236.5 13 35722.5 14 12572.0 15 12481.5 16 13681.5 17 15122.0 18 16450.0 19 17893.5 20 19592.5 21 21738.5 22 24725.0 23 28921.0 24 33043.0 25 37170.5 26 44305.5 27 51614.0 28 55353.0 29 58631.0 30 63416.0 31 68622.0 32 74836.5 33 82499.0 34 91014.5 35 98051.0 36 104215.0 37 109350.0 38 112353.0 39 114368.5 40 117089.5 41 120664.0 42 122677.0 43 123068.5 44 123672.5 45 124226.5 46 123996.0 47 124825.5 48 124611.5 49 128584.0 50 150696.5 51 146999.5 52 116048.0 53 102282.0 54 96191.0 55 91627.5 56 83389.0 57 73605.0 58 69609.5 59 59565.5 60 45088.0 61 36397.5 62 28616.5 63 21703.5 64 16204.0 65 11936.0 66 9094.0 67 7010.5 68 5254.0 69 3991.0 70 2904.0 71 2054.0 72 1558.5 73 1141.5 74 823.5 75 632.0 76 483.5 77 323.5 78 209.0 79 131.0 80 79.5 81 39.0 82 19.0 83 15.0 84 9.5 85 9.0 86 11.5 87 9.0 88 5.0 89 5.5 90 2.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05878334525384675 2 0.0 3 2.5078218964951686E-5 4 3.009386275794202E-4 5 0.009730348958401254 6 0.0032852466844086707 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.3793020430723427E-4 16-17 2.7586040861446855E-4 18-19 0.0 20-21 0.0 22-23 3.761732844742752E-5 24-25 0.003686498187847898 26-27 0.006871431996396761 28-29 0.0012037545103176807 30-31 0.005943537894693549 32-33 0.002846377852522016 34-35 0.025191070950293968 36-37 0.02522868827874139 38-39 0.02165504207623578 40-41 0.0024075090206353614 42-43 0.006056389880035832 44-45 0.007561083017932933 46-47 0.0012288327292826326 48-49 0.008601829104978427 50-51 0.010131600461840482 52-53 0.0020814921740909898 54-55 0.011899614898869574 56-57 0.006746040901572004 58-59 0.012426257497133561 60-61 0.00789963897395978 62-63 9.905896491155916E-4 64-65 0.012952900095397546 66-67 0.00557990371970175 68-69 0.002144187721503369 70-71 0.009329097454962026 72-73 0.005266425982639854 74-75 0.007937256302407207 76-77 0.007147292405011231 78-79 0.014745992751391592 80-81 0.00798741274033711 82-83 0.012739735234195457 84-85 0.007034440419668948 86-87 0.013128447628152207 88-89 0.01875850778578386 90-91 0.016990493348754767 92-93 0.01742936218064142 94-95 0.0057679903619388875 96-97 0.010394921760972474 98-99 0.017956004778905407 100 0.01670209383065782 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3987524.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.819162145440444 #Duplication Level Percentage of deduplicated Percentage of total 1 83.88565201794839 51.857407237735444 2 11.59544323308465 14.336411707486201 3 2.328995938548115 4.319297326835345 4 0.7097658154341254 1.755085121184518 5 0.3194589960721195 0.9874343738500987 6 0.219463844435644 0.8140242590537276 7 0.15600161984047967 0.675072260230898 8 0.11700487652280295 0.5786514746856315 9 0.085946777362691 0.4781841989096039 >10 0.4891869528038046 6.527203124701371 >50 0.07296804444786834 2.9520812178862137 >100 0.016129257706816894 1.7161590054697708 >500 0.001950673874506801 0.8483437840795036 >1k 0.0017881176878229194 1.7742413614614674 >5k 1.2191711507883533E-4 0.5429269510293213 >10k+ 1.2191711507883533E-4 9.837476595400886 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 282830 7.092872669857285 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 86345 2.165378816528753 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG 22402 0.5618022612528476 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA 9775 0.2451395903824027 No Hit ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 6406 0.16065107068948048 No Hit TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 5430 0.13617472897968766 No Hit CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 4288 0.10753540292171282 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC 4143 0.10389906117179483 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.76486160334082E-4 0.0 0.0 0.0 0.0 2 4.76486160334082E-4 0.0 0.0 0.0 0.0 3 4.76486160334082E-4 0.0 0.0 0.0 0.0 4 4.76486160334082E-4 0.0 0.0 5.0156437929903365E-5 0.0 5 4.76486160334082E-4 0.0 0.0 1.0031287585980673E-4 0.0 6 4.76486160334082E-4 0.0 0.0 1.2539109482475843E-4 0.0 7 4.76486160334082E-4 0.0 0.0 1.2539109482475843E-4 0.0 8 4.76486160334082E-4 0.0 0.0 1.2539109482475843E-4 0.0 9 5.015643792990337E-4 0.0 0.0 1.5046931378971012E-4 0.0 10-11 5.266425982639854E-4 0.0 0.0 1.8808664223713762E-4 0.0 12-13 5.893381456763646E-4 0.0 0.0 2.2570397068456515E-4 0.0 14-15 6.394945836062679E-4 0.0 0.0 2.5078218964951686E-4 0.0 16-17 6.896510215361714E-4 0.0 0.0 2.883995180969444E-4 0.0 18-19 7.272683499835988E-4 0.0 0.0 3.0093862757942023E-4 0.0 20-21 7.774247879135022E-4 0.0 0.0 3.510950655093236E-4 0.0 22-23 8.777376637733088E-4 0.0 0.0 4.012515034392269E-4 0.0 24-25 9.52972320668164E-4 0.0 0.0 4.137906129217028E-4 0.0 26-27 9.905896491155916E-4 0.0 0.0 4.514079413691303E-4 0.0 28-29 0.001028206977563019 0.0 0.0 5.141034887815095E-4 0.0 30-31 0.001028206977563019 0.0 0.0 5.266425982639854E-4 0.0 32-33 0.0011285198534228258 0.0 0.0 5.391817077464612E-4 0.0 34-35 0.001178676291352729 0.0 0.0 5.517208172289371E-4 0.0 36-37 0.0014169193715197702 0.0 0.0 5.517208172289371E-4 0.0 38-39 0.0018181708749589972 0.0 0.0 5.517208172289371E-4 0.0 40-41 0.0021191095025384172 0.0 0.0 6.771119120536955E-4 0.0 42-43 0.0025078218964951684 0.0 0.0 6.896510215361714E-4 0.0 44-45 0.0028212996335570647 0.0 0.0 7.021901310186472E-4 0.0 46-47 0.003335403122338574 0.0 0.0 7.523465689485506E-4 0.0 48-49 0.00401251503439227 0.0 0.0 8.275812258434056E-4 0.0 50-51 0.004789939822305772 0.0 0.0 8.526594448083572E-4 0.0 52-53 0.005742912142973936 0.0 0.0 9.404332111856881E-4 0.0 54-55 0.006921588434326665 0.0 0.0 9.780505396331156E-4 0.0 56-57 0.008451359791188717 1.2539109482475841E-5 0.0 0.0010031287585980674 0.0 58-59 0.010068904914428101 2.5078218964951683E-5 0.0 0.0010658243060104465 0.0 60-61 0.012188014416966517 2.5078218964951683E-5 0.0 0.0010783634154929223 0.0 62-63 0.014996774941041107 2.5078218964951683E-5 0.0 0.00111598074394035 0.0 64-65 0.01834471717286216 2.5078218964951683E-5 0.0 0.0011410589629053016 0.0 66-67 0.02234469309777195 2.5078218964951683E-5 0.0 0.001203754510317681 0.0 68-69 0.027598579970929328 2.5078218964951683E-5 0.0 0.0012413718387651084 0.0 70-71 0.03454524662422094 2.5078218964951683E-5 0.0 0.0012789891672125358 0.0 72-73 0.042632972240417864 2.5078218964951683E-5 0.0 0.0012789891672125358 0.0 74-75 0.05406864008843583 2.5078218964951683E-5 0.0 0.0012789891672125358 0.0 76-77 0.06873939818293257 2.5078218964951683E-5 0.0 0.0012789891672125358 0.0 78-79 0.08774868815836595 2.5078218964951683E-5 0.0 0.0012915282766950116 0.0 80-81 0.11034416344578742 2.5078218964951683E-5 0.0 0.0013166064956599635 0.0 82-83 0.1384819251244632 2.5078218964951683E-5 0.0 0.0013291456051424393 0.0 84-85 0.1738422138650451 2.5078218964951683E-5 0.0 0.0013416847146249151 0.0 86-87 0.21866953026489622 2.5078218964951683E-5 0.0 0.001354223824107391 0.0 88 0.2594090969734602 2.5078218964951683E-5 0.0 0.001354223824107391 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGAGT 81850 0.0 75.07874 1 TACGAGT 1720 0.0 74.35904 1 ACGAGTG 85230 0.0 73.69454 2 GTGGAAT 88095 0.0 73.001015 6 CGAGTGG 87195 0.0 72.42721 3 TGGAATC 89335 0.0 72.192856 7 GAATCAC 89800 0.0 71.93414 9 GGAATCA 89810 0.0 71.88951 8 AGTGGAA 88700 0.0 71.68041 5 GAGTGGA 90020 0.0 70.697205 4 CGTGTGG 1670 0.0 48.117825 3 ATCGAGT 795 0.0 45.542503 1 AACGTGT 1480 0.0 44.161762 1 ACGTGTG 1770 0.0 42.21339 2 GAGTACT 40720 0.0 41.59689 20-21 AGTACTT 41620 0.0 41.572422 20-21 GTACTTT 41380 0.0 40.207897 22-23 TCGAGTG 1030 0.0 40.14873 2 CATGGGG 20285 0.0 39.33984 24-25 TACTTTT 45085 0.0 37.88348 22-23 >>END_MODULE