##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132702_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4751651
Sequences flagged as poor quality	0
Sequence length	100
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.92182885485487	33.0	31.0	34.0	25.0	34.0
2	31.64737982650662	34.0	31.0	34.0	28.0	34.0
3	31.828027984378483	34.0	31.0	34.0	28.0	34.0
4	35.70542007399112	37.0	35.0	37.0	35.0	37.0
5	35.30190180213151	37.0	35.0	37.0	33.0	37.0
6	35.55760113695219	37.0	35.0	37.0	35.0	37.0
7	35.71094131281948	37.0	35.0	37.0	35.0	37.0
8	35.77969004878515	37.0	37.0	37.0	35.0	37.0
9	37.60073856434322	39.0	39.0	39.0	35.0	39.0
10-11	37.57561866391282	39.0	39.0	39.0	35.0	39.0
12-13	37.41462262274734	39.0	39.0	39.0	35.0	39.0
14-15	38.84600194753361	41.0	40.0	41.0	36.5	41.0
16-17	38.570927873280255	41.0	39.5	41.0	36.0	41.0
18-19	37.9457692705125	41.0	37.0	41.0	35.5	41.0
20-21	37.628918769497176	40.5	36.5	41.0	34.5	41.0
22-23	37.42981407935894	40.0	36.0	41.0	34.0	41.0
24-25	37.19480418490331	40.0	35.0	41.0	34.0	41.0
26-27	37.12158626548961	40.0	35.0	41.0	33.5	41.0
28-29	36.97559564033638	40.0	35.0	41.0	33.0	41.0
30-31	36.81314336848392	40.0	35.0	41.0	33.0	41.0
32-33	36.64943500690602	40.0	35.0	41.0	33.0	41.0
34-35	36.52155913807643	40.0	35.0	41.0	32.0	41.0
36-37	36.26690112552458	39.5	35.0	41.0	31.5	41.0
38-39	35.829719501705824	39.0	35.0	41.0	31.0	41.0
40-41	35.665956632757755	39.0	35.0	41.0	31.0	41.0
42-43	35.55012057914186	39.0	35.0	41.0	30.5	41.0
44-45	35.37236646799187	38.0	35.0	41.0	30.0	41.0
46-47	35.25703760650772	38.0	35.0	41.0	30.0	41.0
48-49	35.06591614156848	38.0	35.0	41.0	28.5	41.0
50-51	34.78169956084738	38.0	35.0	41.0	26.5	41.0
52-53	34.252334609591486	37.5	35.0	40.5	23.0	41.0
54-55	33.35520327566145	37.0	34.5	40.0	10.0	41.0
56-57	32.873479239110786	37.0	34.5	40.0	2.0	41.0
58-59	32.70306531350893	36.5	34.5	40.0	2.0	41.0
60-61	32.482731370633076	36.0	34.0	40.0	2.0	41.0
62-63	32.29658449242169	35.5	34.0	40.0	2.0	41.0
64-65	31.98469973910121	35.0	33.5	39.5	2.0	41.0
66-67	31.590159188879824	35.0	33.0	39.0	2.0	41.0
68-69	31.40111594896174	35.0	33.0	39.0	2.0	41.0
70-71	31.188780383912878	35.0	33.0	38.0	2.0	40.5
72-73	30.927915897021897	35.0	33.0	37.0	2.0	40.0
74-75	30.64414200453695	35.0	33.0	37.0	2.0	40.0
76-77	30.348000936937495	35.0	32.0	36.0	2.0	39.0
78-79	30.05896771459015	35.0	32.0	36.0	2.0	39.0
80-81	29.776195684405273	35.0	32.0	35.5	2.0	39.0
82-83	29.40344471847785	35.0	31.0	35.0	2.0	37.0
84-85	29.090336811352515	35.0	30.5	35.0	2.0	37.0
86-87	29.04892899331201	35.0	31.0	35.0	2.0	37.0
88-89	29.119132697245654	35.0	32.0	35.0	2.0	36.0
90-91	28.966083262428157	35.0	31.5	35.0	2.0	36.0
92-93	28.839715606217712	35.0	31.5	35.0	2.0	36.0
94-95	28.620169810451145	35.0	31.0	35.0	2.0	35.5
96-97	28.339989931920506	35.0	30.5	35.0	2.0	35.0
98-99	28.246367736182645	35.0	31.0	35.0	2.0	35.0
100	28.22362795584103	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.7964222246445232
1101	2	1.3472638183210357
1101	3	1.2064816247170675
1101	4	0.607753159626796
1101	5	0.8128956595669479
1101	6	0.3976560699898215
1101	7	0.15198311832181588
1101	8	0.1046204996271598
1101	9	0.18636442919701324
1101	10-11	0.18145206865916208
1101	12-13	0.3274897363813025
1101	14-15	0.5288150989516964
1101	16-17	0.5667326628996179
1101	18-19	0.5299993855071605
1101	20-21	0.6916184522518023
1101	22-23	0.6674051329083
1101	24-25	0.6470282889491656
1101	26-27	0.6965538619782734
1101	28-29	0.7229825379731452
1101	30-31	0.7074734419135638
1101	32-33	0.6788156785770383
1101	34-35	0.6272894995324307
1101	36-37	0.6883254988057104
1101	38-39	0.712949450899977
1101	40-41	0.7128656019206332
1101	42-43	0.6871905340760307
1101	44-45	0.7136459483540847
1101	46-47	0.7037498040994308
1101	48-49	0.6678605167663179
1101	50-51	0.6195399117859566
1101	52-53	0.5890494084429889
1101	54-55	0.4497450212587566
1101	56-57	0.4466295173532302
1101	58-59	0.425350500727653
1101	60-61	0.34706788587707393
1101	62-63	0.3796202409964451
1101	64-65	0.4086780624887183
1101	66-67	0.46040327736773534
1101	68-69	0.520860461315209
1101	70-71	0.5215452345941003
1101	72-73	0.5244956203381328
1101	74-75	0.48049794712670035
1101	76-77	0.5344276895035449
1101	78-79	0.5229693336851753
1101	80-81	0.537146674282873
1101	82-83	0.47921577053982034
1101	84-85	0.5304912702001054
1101	86-87	0.5606716117204442
1101	88-89	0.5517458354782505
1101	90-91	0.5355744794522401
1101	92-93	0.573694088234312
1101	94-95	0.595740804639405
1101	96-97	0.6067651298490162
1101	98-99	0.6519432141283659
1101	100	0.6826485725681906
1102	1	1.2329598004654372
1102	2	1.0658361674747034
1102	3	1.0534378014246464
1102	4	0.4912876024893862
1102	5	0.7225437227230813
1102	6	0.3077766246664382
1102	7	0.07768742938745277
1102	8	0.0682683621693343
1102	9	0.08559194208115173
1102	10-11	0.1489683383154059
1102	12-13	0.29923652998173367
1102	14-15	0.4631389873486427
1102	16-17	0.5565405114950508
1102	18-19	0.5671785928698156
1102	20-21	0.6339551039406928
1102	22-23	0.6550700271559933
1102	24-25	0.6652890695743494
1102	26-27	0.6289894005558097
1102	28-29	0.735890072088317
1102	30-31	0.7477109634107819
1102	32-33	0.6811118324027845
1102	34-35	0.6864196615942149
1102	36-37	0.7454072640456744
1102	38-39	0.7338611981550187
1102	40-41	0.6666377056985056
1102	42-43	0.6927446166255891
1102	44-45	0.7595794257034072
1102	46-47	0.6926881528146112
1102	48-49	0.6884442120828282
1102	50-51	0.7353614808462012
1102	52-53	0.7553958618386787
1102	54-55	0.716107847023558
1102	56-57	0.6892817597200604
1102	58-59	0.7163517556136085
1102	60-61	0.7510793383924508
1102	62-63	0.6928597628344377
1102	64-65	0.6468414750185048
1102	66-67	0.6898994389910236
1102	68-69	0.6967723634106662
1102	70-71	0.7375602863163522
1102	72-73	0.6940251732046612
1102	74-75	0.6972964790174707
1102	76-77	0.7143975332529067
1102	78-79	0.7526666891465794
1102	80-81	0.7574903607851908
1102	82-83	0.7901765596411288
1102	84-85	0.7838861309048042
1102	86-87	0.7305483032286091
1102	88-89	0.7195856290704228
1102	90-91	0.7880304643674556
1102	92-93	0.8281297479698466
1102	94-95	0.8323252150595337
1102	96-97	0.8614643514150409
1102	98-99	0.9358134941328906
1102	100	0.9192847527402535
1103	1	1.7185660001292717
1103	2	1.2698247286420958
1103	3	1.1558892799649563
1103	4	0.5379784576058668
1103	5	0.6675722591794084
1103	6	0.25102945535252985
1103	7	0.053624583756352706
1103	8	0.013868432086383109
1103	9	0.060663104647773025
1103	10-11	0.09906780564209328
1103	12-13	0.2462354570959775
1103	14-15	0.39755007246629503
1103	16-17	0.4477466778330452
1103	18-19	0.44115790179309045
1103	20-21	0.5565790197252198
1103	22-23	0.5962169548815268
1103	24-25	0.5534796910137416
1103	26-27	0.541708096919777
1103	28-29	0.6388832396895907
1103	30-31	0.6187159165487088
1103	32-33	0.5730495466843166
1103	34-35	0.5494076358377527
1103	36-37	0.5808362280716466
1103	38-39	0.6871774259813535
1103	40-41	0.6532503915372132
1103	42-43	0.6943774750456058
1103	44-45	0.7086401974728602
1103	46-47	0.6666638602415347
1103	48-49	0.7120447936524528
1103	50-51	0.724176325637913
1103	52-53	0.7788534640032196
1103	54-55	0.7142741331521094
1103	56-57	0.7345939020089318
1103	58-59	0.715213778807005
1103	60-61	0.7002873033177153
1103	62-63	0.7214069756304085
1103	64-65	0.6297037306284636
1103	66-67	0.7199004678184799
1103	68-69	0.735444318834702
1103	70-71	0.7155594294003151
1103	72-73	0.7238571117784964
1103	74-75	0.7857196250661929
1103	76-77	0.8063517784026821
1103	78-79	0.7498696950514727
1103	80-81	0.8102064592697893
1103	82-83	0.8312789008418484
1103	84-85	0.796634587633605
1103	86-87	0.7914930405480725
1103	88-89	0.8234832764649624
1103	90-91	0.8640919423903455
1103	92-93	0.8479488183972279
1103	94-95	0.8842264749950104
1103	96-97	0.8979445081905801
1103	98-99	0.9267403524239732
1103	100	0.9272094132782129
1104	1	1.7819758041031761
1104	2	1.3186684689485269
1104	3	1.1818593264447124
1104	4	0.6265458124528678
1104	5	0.7667617759457599
1104	6	0.2867081069582653
1104	7	-0.008213845702030653
1104	8	-0.09227003697196068
1104	9	-0.06981909206979253
1104	10-11	-0.02954413025469904
1104	12-13	0.11644274269684729
1104	14-15	0.29913021850587995
1104	16-17	0.3339643938614003
1104	18-19	0.35935444942742834
1104	20-21	0.4126602386344729
1104	22-23	0.44368486789651485
1104	24-25	0.44206277218322754
1104	26-27	0.4138339030277294
1104	28-29	0.4617874614944597
1104	30-31	0.49112940582045894
1104	32-33	0.4614538201261169
1104	34-35	0.4472383629383998
1104	36-37	0.5103091451875486
1104	38-39	0.5474204721961016
1104	40-41	0.5411140842892195
1104	42-43	0.5725207888919925
1104	44-45	0.637339492252579
1104	46-47	0.6195219609722642
1104	48-49	0.5421421310174281
1104	50-51	0.6159550505042688
1104	52-53	0.7035270694438651
1104	54-55	0.6810447421460353
1104	56-57	0.7779722677785088
1104	58-59	0.7191026314390143
1104	60-61	0.7078148361442089
1104	62-63	0.7416765329963866
1104	64-65	0.7288464900008762
1104	66-67	0.7133249341658079
1104	68-69	0.6916768476986306
1104	70-71	0.7610848271549315
1104	72-73	0.7734435318567989
1104	74-75	0.7258212256806722
1104	76-77	0.7837490096103217
1104	78-79	0.748921049832699
1104	80-81	0.7227693173947429
1104	82-83	0.7378553218951289
1104	84-85	0.7249376615380498
1104	86-87	0.8078318441354817
1104	88-89	0.7776366792875855
1104	90-91	0.8146245760700275
1104	92-93	0.828651174313066
1104	94-95	0.8256301089028071
1104	96-97	0.8611683446744642
1104	98-99	0.875256630262438
1104	100	0.8883158327341718
1105	1	1.505148940768656
1105	2	1.2424441797403283
1105	3	1.1752867650844259
1105	4	0.605508553284551
1105	5	0.987751911293195
1105	6	0.6067069574649437
1105	7	0.2537517626470418
1105	8	0.060870399491371074
1105	9	0.025448409709170505
1105	10-11	0.09168917337738947
1105	12-13	0.18612217189234315
1105	14-15	0.342283715591563
1105	16-17	0.4065922373822062
1105	18-19	0.4720890697068185
1105	20-21	0.49503602871969576
1105	22-23	0.5770217461014866
1105	24-25	0.5712423988014947
1105	26-27	0.6844444037933215
1105	28-29	0.7759922260721979
1105	30-31	0.6824770047622408
1105	32-33	0.7043774771039821
1105	34-35	0.693215068762612
1105	36-37	0.7610581175576385
1105	38-39	0.7579354893443693
1105	40-41	0.7268392555713845
1105	42-43	0.7771119066418137
1105	44-45	0.8172992473744642
1105	46-47	0.831217821255855
1105	48-49	0.7920426088589139
1105	50-51	0.7763687798146321
1105	52-53	0.7179824208622563
1105	54-55	0.6133426711752676
1105	56-57	0.6262564012663887
1105	58-59	0.5888476294990213
1105	60-61	0.5896839257914408
1105	62-63	0.6682150590847087
1105	64-65	0.5750834263381392
1105	66-67	0.64106536091791
1105	68-69	0.6752221121059705
1105	70-71	0.646573266774503
1105	72-73	0.6909920697976908
1105	74-75	0.6743114031431645
1105	76-77	0.7119593007645477
1105	78-79	0.6355523389507169
1105	80-81	0.6879607274005437
1105	82-83	0.7288583295858331
1105	84-85	0.7737428190875484
1105	86-87	0.7074190309965225
1105	88-89	0.6865144927422975
1105	90-91	0.6630989930422366
1105	92-93	0.7696995311366912
1105	94-95	0.7050960278222718
1105	96-97	0.7131870927663364
1105	98-99	0.662449180212473
1105	100	0.7472321938527209
1106	1	1.5288811291138273
1106	2	1.2045060850087133
1106	3	1.0761050688639067
1106	4	0.6542580700831735
1106	5	1.0096421539737506
1106	6	0.6922571938803728
1106	7	0.3789013474064262
1106	8	0.19489937153514347
1106	9	0.017373829963773346
1106	10-11	-0.055687524849581393
1106	12-13	-0.0031979901036507385
1106	14-15	0.17845928780732834
1106	16-17	0.235670743882757
1106	18-19	0.28741102355138537
1106	20-21	0.3237346751392636
1106	22-23	0.3747471842711505
1106	24-25	0.38804110340218045
1106	26-27	0.3734090691627756
1106	28-29	0.45926870991790025
1106	30-31	0.47802120726202446
1106	32-33	0.4529018610254383
1106	34-35	0.44593741718382063
1106	36-37	0.423521917061052
1106	38-39	0.4507717816599168
1106	40-41	0.46200561557537867
1106	42-43	0.4906869365758979
1106	44-45	0.5134463790037387
1106	46-47	0.49533769339096523
1106	48-49	0.5011741557558693
1106	50-51	0.4676899564264687
1106	52-53	0.5242156287045248
1106	54-55	0.4776125177137729
1106	56-57	0.4689300792985023
1106	58-59	0.4259085071348707
1106	60-61	0.41667426159263954
1106	62-63	0.406391418980796
1106	64-65	0.40657076770378353
1106	66-67	0.36822061889391833
1106	68-69	0.37348768677605904
1106	70-71	0.36425964035009883
1106	72-73	0.4391534639387835
1106	74-75	0.42742825540882734
1106	76-77	0.4321746565746025
1106	78-79	0.41057371370723317
1106	80-81	0.44461608240219164
1106	82-83	0.4859643656766295
1106	84-85	0.49202821678904485
1106	86-87	0.4931200125397268
1106	88-89	0.46230725557173
1106	90-91	0.4782508094769291
1106	92-93	0.4935052851252344
1106	94-95	0.42986191172411736
1106	96-97	0.41060290097448515
1106	98-99	0.3653970768456567
1106	100	0.3901875673688835
1107	1	1.5801202996500194
1107	2	1.2222526785872567
1107	3	1.1507836834104204
1107	4	0.6749735653172664
1107	5	1.0258790132218891
1107	6	0.7484142178920621
1107	7	0.559509592083792
1107	8	0.4657772389996282
1107	9	0.2861573503101482
1107	10-11	0.10359848116218728
1107	12-13	0.030179568138137824
1107	14-15	0.1334089972835102
1107	16-17	0.18018430869413038
1107	18-19	0.16604348072949904
1107	20-21	0.23099011879257603
1107	22-23	0.28431094502057164
1107	24-25	0.3084637344289334
1107	26-27	0.32687384275597964
1107	28-29	0.3340891182276735
1107	30-31	0.3309243354315612
1107	32-33	0.36668335869159563
1107	34-35	0.38138572460793085
1107	36-37	0.42983230340267653
1107	38-39	0.36266398929652865
1107	40-41	0.3765385850202989
1107	42-43	0.4112919483603079
1107	44-45	0.41786552296861146
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2216	90-91	-1.908632586792283
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2216	94-95	-2.0639968110735865
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2216	98-99	-2.2716326195803767
2216	100	-2.25681039336569
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	14796.0
3	23076.0
4	29276.0
5	39252.0
6	27135.0
7	18089.0
8	15325.0
9	15840.0
10	14486.0
11	18235.0
12	13662.0
13	15669.0
14	14187.0
15	14927.0
16	17651.0
17	21404.0
18	26729.0
19	38953.0
20	78488.0
21	110265.0
22	48144.0
23	27044.0
24	25169.0
25	26609.0
26	28828.0
27	32012.0
28	38759.0
29	45030.0
30	52799.0
31	65668.0
32	86130.0
33	121946.0
34	185775.0
35	808210.0
36	524884.0
37	803374.0
38	866014.0
39	391490.0
40	6321.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.336746109773875	51.70251022474757	23.19806393617879	13.762679729299768
2	13.751536045050447	49.51102258983246	22.23660786535038	14.500833499766713
3	14.857434725804605	30.41508966994188	19.28682427654574	35.440651327707776
4	33.33391558942275	31.990629745737888	20.64875815298828	14.026696511851085
5	13.533513021347456	48.562994006985186	22.177459242910945	15.726033728756411
6	35.798945455723995	28.853996505617236	22.56221344019921	12.784844598459557
7	15.492972863537327	29.319577553149422	40.98609093975967	14.201358643553577
8	35.20671025712957	30.029436084426237	21.179459518386345	13.584394140057846
9	34.638444616408066	31.643233057309978	21.51294360633809	12.205378719943868
10-11	12.850754400944009	51.95123758036944	21.537250947091863	13.660757071594695
12-13	12.861192878012295	30.739862839253135	31.5260843020668	24.872859980667773
14-15	13.497835012243659	40.99757273457068	20.743885773417134	24.760706479768526
16-17	24.78144891179562	30.152589582322715	30.85763111013367	14.20833039574799
18-19	14.427325244914924	30.899003504045965	30.739395788831246	23.934275462207864
20-21	25.10064396564478	41.55037901563057	20.534462653086262	12.814514365638384
22-23	24.24142395182978	31.00555922737988	30.958491361789207	13.794525459001134
24-25	13.805674168145684	41.40160620002724	20.325308226118988	24.467411405708095
26-27	14.5983787612035	36.337301015194775	35.45474348626325	13.609576737338477
28-29	26.26662651175457	30.751338812753097	29.50795711253351	13.474077562958819
30-31	21.110910351209746	34.04626834054824	30.36829316211893	14.474528146123088
32-33	15.918618266177994	35.0755765248026	31.04176284226367	17.964042366755738
34-35	18.034211774676475	35.0567489478081	30.953518942085477	15.955520335429945
36-37	18.507362083488626	33.30744809629308	32.92108514646648	15.26410467375181
38-39	20.76127368080263	33.203717526583084	31.20208740915316	14.832921383461128
40-41	15.460936501966838	38.08818122488047	31.514281811785793	14.93660046136689
42-43	15.291227705368222	33.14935824798248	34.15158539603752	17.40782865061178
44-45	15.370408761382398	35.86577792837968	30.948507831006427	17.815305479231494
46-47	18.134093582592556	33.17954301493634	33.117911137081315	15.56845226538979
48-49	16.112495257799438	33.3323265748973	33.03416120798384	17.521016959319414
50-51	18.376670972630382	35.89373127103104	30.756641137826797	14.972956618511784
52-53	18.166956045921857	33.680009681051445	32.61577800928118	15.537256263745514
54-55	16.340888581358577	36.639650187193	28.90530375780409	18.11415747364433
56-57	16.91868671545954	37.134086325903695	29.672478644567068	16.274748314069694
58-59	21.748600180065175	35.163592526505944	26.94246491439011	16.14534237903877
60-61	19.103558895850526	37.56402752354788	26.876542874684006	16.45587070591759
62-63	16.812296667025496	37.11888204311961	26.781566995429852	19.287254294425043
64-65	18.758561987129273	37.21449387283495	27.316344751210504	16.71059938882527
66-67	19.202819626767553	34.91111794417193	29.54097405632729	16.345088372733223
68-69	21.13853288704958	35.18168651147755	27.69945339780652	15.98032720366635
70-71	15.904626772389033	39.75294288376141	27.795709318148837	16.546721025700712
72-73	15.930266477136643	35.463143299598734	29.76756123771468	18.839028985549948
74-75	16.289789326343744	37.589777529469295	26.915216252704415	19.20521689148254
76-77	19.132916765368023	35.10123733882125	28.567931773411637	17.197914122399087
78-79	17.277709463552547	35.56023441344649	28.138138239272294	19.023917883728675
80-81	19.678962817519956	37.79651761591907	26.24814088652128	16.27637868003969
82-83	19.213984369014515	36.02064037704301	28.15531681665374	16.610058437288732
84-85	17.480892091751635	38.06408976060261	25.650479184139936	18.804538963505816
86-87	17.514335309535973	38.04055372402327	27.877549104604878	16.567561861835884
88-89	22.145617911116315	35.73123642845295	25.850076661612025	16.27306899881871
90-91	19.37874461987162	38.221202420805184	25.89997171012306	16.50008124920014
92-93	17.169639395359233	38.006387904153655	25.743413710461322	19.080558990025793
94-95	19.066340964149187	38.35017706996329	25.518731911140165	17.064750054747364
96-97	19.510176883259323	35.99889669618177	27.523620688693523	16.96730573186539
98-99	21.677572455066375	36.31685421591671	25.476699809570558	16.528873519446346
100	16.94341621085503	40.454982312698355	25.74151744124124	16.86008403520537
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	641535.0
1	337878.5
2	23688.0
3	10109.5
4	6537.0
5	5744.5
6	5897.5
7	7097.5
8	6699.0
9	5810.5
10	6308.5
11	9634.5
12	76993.5
13	81943.0
14	17986.5
15	13364.5
16	14323.0
17	15637.5
18	16734.5
19	18139.5
20	19735.5
21	21965.5
22	24861.5
23	27625.5
24	30486.5
25	33952.5
26	38700.5
27	44025.5
28	48522.5
29	53196.5
30	58236.5
31	63210.0
32	67871.5
33	72487.0
34	78041.0
35	83201.0
36	86571.5
37	90189.5
38	92861.0
39	93428.5
40	93254.0
41	91835.0
42	90895.5
43	90849.0
44	91054.0
45	92282.5
46	97283.0
47	113808.0
48	126385.0
49	176439.0
50	383007.0
51	384652.0
52	165120.5
53	91769.5
54	80437.0
55	79180.0
56	70941.0
57	56493.5
58	46543.5
59	37817.0
60	31135.0
61	25290.5
62	20223.0
63	16183.0
64	12400.5
65	9521.0
66	8097.5
67	7072.5
68	5980.0
69	5070.0
70	4353.5
71	3699.5
72	3044.0
73	2483.5
74	1979.5
75	1494.5
76	1073.0
77	734.0
78	490.5
79	330.5
80	201.5
81	119.0
82	64.0
83	35.5
84	25.0
85	14.0
86	7.5
87	7.0
88	4.5
89	2.0
90	0.5
91	0.5
92	1.0
93	0.5
94	0.5
95	1.0
96	0.5
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05543336410860141
2	0.0
3	6.31359500097966E-5
4	3.9986101672871173E-4
5	0.009533528451479286
6	5.26132916748305E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.5783987502449148E-4
16-17	3.367250667189152E-4
18-19	2.10453166699322E-5
20-21	0.0
22-23	3.15679750048983E-5
24-25	0.003956519533947253
26-27	0.007281679567796541
28-29	0.0011154017835064065
30-31	0.005461259675847405
32-33	0.0029989576254653384
34-35	0.02547535582895293
36-37	0.025159676078903945
38-39	0.02258162478683725
40-41	0.0024833473670519994
42-43	0.005955824617590812
44-45	0.007281679567796541
46-47	0.0010838338085015081
48-49	0.009017918193065947
50-51	0.010280637193261877
52-53	0.002125576983663152
54-55	0.01133290302675849
56-57	0.007208020959451778
58-59	0.01249039544360476
60-61	0.007870948434554643
62-63	0.0011469697585113048
64-65	0.013395344060411846
66-67	0.005092966634123592
68-69	0.0024202114170422027
70-71	0.008449694642977778
72-73	0.005240283850813117
74-75	0.008260286792948388
76-77	0.007692063242860218
78-79	0.015342035852380573
80-81	0.008302377426288253
82-83	0.012101057085211014
84-85	0.007281679567796541
86-87	0.013048096335357962
88-89	0.019003920952948776
90-91	0.017572839419393388
92-93	0.01677311738593596
94-95	0.005903211325915982
96-97	0.00981764022652337
98-99	0.01704670650264508
100	0.016520573585896776
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4751651.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.82222689329912
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.04184314805441	22.513122622140987
2	11.939240503386134	7.3598795945806845
3	5.797969543960573	5.361189984131723
4	3.3351142859959806	4.111825969522056
5	1.953790390126265	3.0110085353209572
6	1.1724584427382925	2.1682668087026284
7	0.6906574023510531	1.4901319412560519
8	0.43314430249131625	1.0680377575141708
9	0.28110457797296956	0.7797842174725165
>10	1.1677208118607258	6.035190026989364
>50	0.08364580094066106	1.8356844580734013
>100	0.0896507249592277	5.478356118792309
>500	0.00569741411326835	1.1861088547583545
>1k	0.003912681981102845	2.6995025932190875
>5k	0.0023338804799560827	5.077051984715367
>10k+	0.001716088588203002	29.824858532810406
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	619894	13.045865531790952	No Hit
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	352856	7.4259662588855955	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	173010	3.6410502370649698	No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT	18768	0.3949785032612875	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14943	0.31448016699879683	No Hit
ACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGA	13872	0.2919406328452995	No Hit
TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG	13357	0.28110229476028437	No Hit
AGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTC	12388	0.26070938290712004	No Hit
CCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGAT	12319	0.25925725605689476	No Hit
TCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGT	12300	0.258857395040166	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12124	0.25515341930625796	No Hit
TCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCA	12111	0.25487983018954885	No Hit
CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA	11911	0.2506707668555624	No Hit
TACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACT	11663	0.2454515283214192	No Hit
TCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGA	11413	0.24019019915393616	No Hit
TCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACT	11118	0.23398183073630616	No Hit
CCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATT	11077	0.23311897275283897	No Hit
TGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCA	11039	0.23231925071938156	No Hit
CTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCG	11029	0.2321087975526822	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10932	0.23006740183569876	No Hit
CCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTC	10913	0.2296675408189701	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGTAACGAGTGGAATCACTGCTG	10301	0.2167878070169716	No Hit
CGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAG	10150	0.21360996419981182	No Hit
ACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTC	10143	0.21346264698312226	No Hit
ATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTA	10030	0.21108452619941995	No Hit
CTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGC	8882	0.1869245026623378	No Hit
GAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGT	8711	0.18332575351177938	No Hit
CTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACG	8645	0.18193676261156386	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8525	0.17941132461117199	No Hit
CAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTT	8499	0.17886414637775375	No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8481	0.17848533067769495	No Hit
TGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATG	8364	0.17602302862731292	No Hit
ACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAG	8286	0.17438149392705818	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	8265	0.17393954227698963	No Hit
GTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCAT	8171	0.171961282510016	No Hit
GTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCAC	8159	0.1717087387099768	No Hit
ATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGG	8040	0.1692043460262549	No Hit
TGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGA	7990	0.16815208019275826	No Hit
GATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGT	7895	0.1661527751091147	No Hit
CTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGT	7523	0.1583239173078999	No Hit
TGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTG	7330	0.154262171190603	No Hit
CGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAG	7305	0.1537360382738547	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6570	0.13826773052145455	No Hit
ACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATC	6566	0.1381835492547748	No Hit
ATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCC	6544	0.13772055228803629	No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6527	0.13736278190464746	No Hit
ATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCAC	6513	0.1370681474712684	No Hit
ACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAAC	6369	0.13403762187079818	No Hit
TTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTAC	6066	0.1276608909198087	No Hit
TACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAAT	5982	0.12589308431953442	No Hit
TCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGA	5942	0.1250512716527371	No Hit
GGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGC	5717	0.12031607540200237	No Hit
TGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACA	5659	0.1190954470351463	No Hit
AGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCA	5627	0.11842199690170847	No Hit
GAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATG	5581	0.1174539123348916	No Hit
AGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGA	5456	0.11482324775115008	No Hit
TGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACT	5392	0.11347634748427442	No Hit
GGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACAT	5298	0.11149808771730078	No Hit
GCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCC	5085	0.10701543526660522	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGAAACGAGTGGAATCACTGCTG	4768	0.10034406988223671	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.003493522567208745	0.0	0.0	0.0	0.0
2	0.003514567883878677	0.0	0.0	0.0	0.0
3	0.003514567883878677	0.0	0.0	0.0	0.0
4	0.003514567883878677	0.0	0.0	0.0	0.0
5	0.003514567883878677	0.0	0.0	0.0	0.0
6	0.003514567883878677	0.0	0.0	0.0	0.0
7	0.003514567883878677	0.0	0.0	0.0	0.0
8	0.0035356132005486094	0.0	0.0	0.0	0.0
9	0.0035566585172185413	0.0	0.0	0.0	0.0
10-11	0.003651362442233236	0.0	0.0	0.0	0.0
12-13	0.0038828609256024906	0.0	0.0	0.0	0.0
14-15	0.004356380550675965	0.0	0.0	0.0	0.0
16-17	0.004798332200744541	0.0	0.0	0.0	0.0
18-19	0.005145579925798422	0.0	0.0	0.0	0.0
20-21	0.005661190184211761	0.0	0.0	0.0	0.0
22-23	0.0062399363926348966	0.0	0.0	0.0	0.0
24-25	0.006871295892732863	0.0	0.0	0.0	0.0
26-27	0.007607881976180489	0.0	0.0	0.0	0.0
28-29	0.0080393109679141	0.0	0.0	0.0	0.0
30-31	0.008670670468012065	0.0	0.0	0.0	0.0
32-33	0.009186280726425404	0.0	0.0	0.0	0.0
34-35	0.009722936301508675	0.0	0.0	0.0	0.0
36-37	0.010533180993301066	0.0	0.0	0.0	0.0
38-39	0.01138551631843332	0.0	0.0	0.0	0.0
40-41	0.012500918101939726	0.0	0.0	0.0	0.0
42-43	0.013753114443800691	0.0	0.0	0.0	0.0
44-45	0.015121060027346284	0.0	0.0	0.0	0.0
46-47	0.01647848295255691	0.0	0.0	0.0	0.0
48-49	0.01769911131941298	0.0	2.10453166699322E-5	0.0	0.0
50-51	0.019414304628012455	0.0	4.20906333398644E-5	0.0	0.0
52-53	0.021087407303272063	0.0	4.20906333398644E-5	0.0	0.0
54-55	0.023212984286935214	0.0	4.20906333398644E-5	0.0	0.0
56-57	0.025349083928933334	0.0	4.20906333398644E-5	0.0	0.0
58-59	0.0278113859793154	0.0	4.20906333398644E-5	0.0	0.0
60-61	0.031136546013164686	0.0	4.20906333398644E-5	0.0	0.0
62-63	0.03510358820544691	0.0	4.20906333398644E-5	0.0	0.0
64-65	0.04033334939792506	0.0	4.20906333398644E-5	0.0	0.0
66-67	0.0457840864154375	0.0	4.20906333398644E-5	0.0	0.0
68-69	0.052434406483136076	0.0	4.20906333398644E-5	0.0	0.0
70-71	0.06203107088462515	0.0	4.20906333398644E-5	0.0	0.0
72-73	0.0723853666862318	0.0	4.20906333398644E-5	0.0	0.0
74-75	0.08538084972991493	0.0	4.20906333398644E-5	0.0	0.0
76-77	0.10068079494895563	0.0	4.20906333398644E-5	0.0	0.0
78-79	0.11945321741853515	0.0	4.20906333398644E-5	0.0	0.0
80-81	0.14445505362241462	1.05226583349661E-5	4.20906333398644E-5	0.0	0.0
82-83	0.17581257546061357	2.10453166699322E-5	4.20906333398644E-5	0.0	0.0
84-85	0.21323114849975303	2.10453166699322E-5	4.20906333398644E-5	0.0	1.05226583349661E-5
86-87	0.25938352795691433	2.10453166699322E-5	5.2613291674830494E-5	0.0	2.10453166699322E-5
88	0.3001903969799129	4.20906333398644E-5	6.313595000979659E-5	0.0	2.10453166699322E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	2415	0.0	71.841606	1
AACGTGT	1680	0.0	64.0904	1
CGTGTGG	2060	0.0	63.419144	3
ACGTGTG	1980	0.0	62.421375	2
TCGAGTG	1305	0.0	50.054955	2
ATCTGCG	2290	0.0	42.280785	94
GTGGAAT	224935	0.0	42.05558	6
TGGAATC	228540	0.0	41.543915	7
AGTGGAA	225895	0.0	41.2762	5
GGAATCA	230605	0.0	41.192276	8
GAATCAC	231370	0.0	41.062172	9
ACGATTG	655	0.0	40.895576	2
AACGAGT	228895	0.0	39.39639	1
GTGTGGA	3565	0.0	39.15071	4
AGTACTT	54695	0.0	39.14217	20-21
ACGAGTG	235435	0.0	39.084686	2
CGAGTGG	239800	0.0	38.63976	3
GAGTGGA	243200	0.0	38.339172	4
GAGTACT	54615	0.0	37.870052	20-21
TACTTTT	55830	0.0	37.51006	22-23
>>END_MODULE