FastQCFastQC Report
Thu 26 May 2016
SRR2132701_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132701_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5504289
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4053307.363893865311215No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1861793.3824350429274332No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG366160.6652266986708001No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT93500.16986753420832373No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA86060.1563508020745277No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT76540.1390551985914984No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC61830.11233058438610327No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT56250.10219303528575625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT22100.079.568681
AACGAGT1202150.072.520491
ACGAGTG1248600.071.33452
GTGGAAT1302250.070.434016
CGAGTGG1278200.070.3075563
TGGAATC1312750.070.010247
GAATCAC1314000.069.947219
GGAATCA1317900.069.768758
AGTGGAA1302700.069.425365
GAGTGGA1319000.068.599484
AACGTGT19800.052.4795271
CGTGTGG24150.050.980753
TCGAGTG13750.049.554992
ACGTGTG23650.049.2768172
ATCGAGT13650.042.023291
GAGTACT589550.041.49327520-21
AGTACTT603400.041.3508320-21
GTACTTT591000.040.58642222-23
CATGGGG279150.039.1939224-25
TACTTTT637200.038.40706322-23