##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132701_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5504289
Sequences flagged as poor quality	0
Sequence length	100
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.87681624275179	33.0	31.0	34.0	25.0	34.0
2	31.619580112890148	34.0	31.0	34.0	28.0	34.0
3	31.81875170435273	34.0	31.0	34.0	28.0	34.0
4	35.76164042258682	37.0	35.0	37.0	35.0	37.0
5	35.41258389594006	37.0	35.0	37.0	33.0	37.0
6	35.75549466970212	37.0	35.0	37.0	35.0	37.0
7	35.93257639633384	37.0	35.0	37.0	35.0	37.0
8	36.062849897598035	37.0	37.0	37.0	35.0	37.0
9	37.97299832912116	39.0	39.0	39.0	37.0	39.0
10-11	38.030182190651686	39.0	39.0	39.0	37.0	39.0
12-13	37.99337443945984	39.0	39.0	39.0	37.0	39.0
14-15	39.49912713522128	41.0	40.0	41.0	37.0	41.0
16-17	39.40857811063336	41.0	39.5	41.0	37.0	41.0
18-19	39.262093432957464	41.0	39.5	41.0	36.0	41.0
20-21	39.122280915845806	41.0	39.0	41.0	35.0	41.0
22-23	39.01759164535147	40.5	39.0	41.0	35.0	41.0
24-25	38.95501698766181	41.0	39.0	41.0	35.0	41.0
26-27	38.8756732250069	41.0	39.0	41.0	35.0	41.0
28-29	38.72704240275175	41.0	39.0	41.0	35.0	41.0
30-31	38.63149055218576	41.0	39.0	41.0	35.0	41.0
32-33	38.489974000275055	41.0	39.0	41.0	35.0	41.0
34-35	38.4075900629491	41.0	38.0	41.0	35.0	41.0
36-37	38.27603301716171	40.5	38.0	41.0	35.0	41.0
38-39	37.84519735428136	40.0	36.5	41.0	34.5	41.0
40-41	37.639499215975036	40.0	35.5	41.0	34.0	41.0
42-43	37.52947392478847	40.0	35.0	41.0	33.5	41.0
44-45	37.371407733133196	40.0	35.0	41.0	33.0	41.0
46-47	37.273893412936715	40.0	35.0	41.0	33.0	41.0
48-49	37.127053285174526	40.0	35.0	41.0	33.0	41.0
50-51	36.89458665778632	40.0	35.0	41.0	33.0	41.0
52-53	36.43818129825669	40.0	35.0	41.0	31.5	41.0
54-55	35.58896671304868	39.5	35.0	41.0	27.5	41.0
56-57	34.9410303492422	39.0	35.0	41.0	22.5	41.0
58-59	34.632682077558066	39.0	35.0	41.0	20.0	41.0
60-61	34.40925649071116	39.0	35.0	41.0	18.0	41.0
62-63	34.16265524938825	38.5	35.0	41.0	17.5	41.0
64-65	33.90266817385497	38.0	35.0	40.5	14.0	41.0
66-67	33.62180156238162	37.0	35.0	40.0	12.0	41.0
68-69	33.34420094947776	37.0	34.0	40.0	9.0	41.0
70-71	33.03185306948818	36.0	34.0	39.0	7.5	41.0
72-73	32.71564356450034	36.0	34.0	39.0	6.0	41.0
74-75	32.385178812377035	35.0	34.0	39.0	2.0	40.5
76-77	32.028912907734316	35.0	34.0	37.5	2.0	40.0
78-79	31.698105422880232	35.0	33.0	37.0	2.0	39.0
80-81	31.376038485624576	35.0	33.0	37.0	2.0	39.0
82-83	31.051322795732563	35.0	33.0	36.0	2.0	39.0
84-85	30.71965661686732	35.0	33.0	36.0	2.0	37.0
86-87	30.595239821164913	35.0	33.0	36.0	2.0	37.0
88-89	30.651018687427204	35.0	33.0	35.0	2.0	37.0
90-91	30.524126640152797	35.0	33.0	35.0	2.0	36.0
92-93	30.384034068705333	35.0	33.0	35.0	2.0	36.0
94-95	30.235182872846977	35.0	33.0	35.0	2.0	36.0
96-97	30.081661228180423	35.0	33.0	35.0	2.0	36.0
98-99	29.96089712949302	35.0	33.0	35.0	2.0	36.0
100	29.88124460761417	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.8574883660490649
1101	2	1.3650340402301921
1101	3	1.225644593236975
1101	4	0.6319419139443596
1101	5	0.9040715062156508
1101	6	0.5133208864628571
1101	7	0.3008145033510772
1101	8	0.2213325018336576
1101	9	0.24389541661120262
1101	10-11	0.1606081520095728
1101	12-13	0.23273868300156408
1101	14-15	0.3181068038754802
1101	16-17	0.32903336209076883
1101	18-19	0.2897351309014269
1101	20-21	0.3839194801204897
1101	22-23	0.3902315238741991
1101	24-25	0.40036329474432364
1101	26-27	0.40465327615971347
1101	28-29	0.41196816877204867
1101	30-31	0.39345341188629135
1101	32-33	0.33595149917175604
1101	34-35	0.2780319786505885
1101	36-37	0.3123244595764376
1101	38-39	0.3164395696593729
1101	40-41	0.3130659900959998
1101	42-43	0.3164039160280012
1101	44-45	0.3551956727383825
1101	46-47	0.3320064519528074
1101	48-49	0.26668766380115727
1101	50-51	0.18871382719035523
1101	52-53	0.17501342097844486
1101	54-55	0.014954132351128635
1101	56-57	0.08823257940139229
1101	58-59	0.011448078934996886
1101	60-61	-0.006903257550696651
1101	62-63	0.005421741894025445
1101	64-65	-0.01981885328670785
1101	66-67	0.10755506575787166
1101	68-69	0.14301716966838995
1101	70-71	0.059095274007738396
1101	72-73	0.055757735150741894
1101	74-75	0.0346412705866328
1101	76-77	0.13874344096606706
1101	78-79	0.1131162510814363
1101	80-81	0.049046327456814254
1101	82-83	0.09490826484656623
1101	84-85	0.09472583196432183
1101	86-87	0.09120370229874197
1101	88-89	0.06845531989009146
1101	90-91	0.08695465798657409
1101	92-93	0.07451784491273017
1101	94-95	0.05172718005380972
1101	96-97	0.05681210134201464
1101	98-99	0.10060620798783759
1101	100	0.10827683698931523
1102	1	1.360230846512767
1102	2	1.164397721680345
1102	3	1.112431469772627
1102	4	0.5646492210980938
1102	5	0.8691236818042682
1102	6	0.5012659213204884
1102	7	0.28409009044753475
1102	8	0.2665240249541654
1102	9	0.26417412333073287
1102	10-11	0.2138736669585981
1102	12-13	0.23130898306036585
1102	14-15	0.35235705000141593
1102	16-17	0.3592133890328313
1102	18-19	0.3779386600153174
1102	20-21	0.40764908012786094
1102	22-23	0.4436318392043077
1102	24-25	0.48360632701011497
1102	26-27	0.4119653712188338
1102	28-29	0.4903984141841491
1102	30-31	0.49832141515180695
1102	32-33	0.4655808207288388
1102	34-35	0.4637713451424901
1102	36-37	0.4514300112770613
1102	38-39	0.42714761002969936
1102	40-41	0.38459278004258834
1102	42-43	0.41497228405985
1102	44-45	0.4326840285232336
1102	46-47	0.3006290274687018
1102	48-49	0.36975156184848856
1102	50-51	0.3757338326229842
1102	52-53	0.352945853333793
1102	54-55	0.33101299022757047
1102	56-57	0.3174745790040703
1102	58-59	0.3413830404443061
1102	60-61	0.32329788747019705
1102	62-63	0.28553210372363935
1102	64-65	0.28177266362446574
1102	66-67	0.34163137117982245
1102	68-69	0.3293350316513397
1102	70-71	0.2798929383531643
1102	72-73	0.3366077772058915
1102	74-75	0.3131233963969393
1102	76-77	0.31631070433413555
1102	78-79	0.33419231980359854
1102	80-81	0.3154587948302421
1102	82-83	0.29096868176632285
1102	84-85	0.28057663437978775
1102	86-87	0.19799229752601377
1102	88-89	0.2079915334658864
1102	90-91	0.24446860306215257
1102	92-93	0.2596073598360391
1102	94-95	0.2606464460340341
1102	96-97	0.26237457646208995
1102	98-99	0.30069488343307427
1102	100	0.29550093263602406
1103	1	1.8110013007184982
1103	2	1.2982534098390133
1103	3	1.2295779180591815
1103	4	0.6012449580857719
1103	5	0.871898682147112
1103	6	0.5141909354579965
1103	7	0.30652663200262964
1103	8	0.20755077800252053
1103	9	0.2263800880064295
1103	10-11	0.1849378932953769
1103	12-13	0.2141412816824655
1103	14-15	0.310465027872624
1103	16-17	0.2644197124350143
1103	18-19	0.29700441522734167
1103	20-21	0.3715786383746149
1103	22-23	0.40427961046872696
1103	24-25	0.3780254735105757
1103	26-27	0.31974548917958856
1103	28-29	0.3199997136146848
1103	30-31	0.3551190240269406
1103	32-33	0.3623891999908082
1103	34-35	0.33244221767683513
1103	36-37	0.3596252579467958
1103	38-39	0.39288094857361244
1103	40-41	0.39874599498210017
1103	42-43	0.38708634471120007
1103	44-45	0.3959129668506378
1103	46-47	0.3372089797354363
1103	48-49	0.375537405340836
1103	50-51	0.35852518720844984
1103	52-53	0.31823948815409153
1103	54-55	0.24123598494160348
1103	56-57	0.22474901018614446
1103	58-59	0.16824114097577336
1103	60-61	0.14953448072562736
1103	62-63	0.1187663550661
1103	64-65	0.11677634179956442
1103	66-67	0.08150119784279752
1103	68-69	0.16986138584549337
1103	70-71	0.0849126669343292
1103	72-73	0.15091463582438536
1103	74-75	0.18661371286160744
1103	76-77	0.230543078317325
1103	78-79	0.22540209828098057
1103	80-81	0.29191795007321986
1103	82-83	0.2280048734763227
1103	84-85	0.16916870979339294
1103	86-87	0.1680494895999587
1103	88-89	0.23071696033303013
1103	90-91	0.17348327798918817
1103	92-93	0.1612792462014454
1103	94-95	0.1718580609352358
1103	96-97	0.19549647530575953
1103	98-99	0.1896827282871243
1103	100	0.21151025203150908
1104	1	1.8794753065710843
1104	2	1.3913499412913097
1104	3	1.2859937822599754
1104	4	0.6776343006190615
1104	5	0.9540865695780738
1104	6	0.5645898591599305
1104	7	0.35437381215399455
1104	8	0.2513891336392504
1104	9	0.25277219963487596
1104	10-11	0.16546066465352993
1104	12-13	0.20709602253742077
1104	14-15	0.2954342849507441
1104	16-17	0.3430085964618286
1104	18-19	0.36219749075940655
1104	20-21	0.35343741114147775
1104	22-23	0.36978931239378454
1104	24-25	0.40228704730915155
1104	26-27	0.3780380492025799
1104	28-29	0.3940886191678601
1104	30-31	0.36934063933509265
1104	32-33	0.3452565547651929
1104	34-35	0.3528189548117524
1104	36-37	0.3899484158248896
1104	38-39	0.4191519557894736
1104	40-41	0.43017184433119127
1104	42-43	0.4188277814211645
1104	44-45	0.40254114657475526
1104	46-47	0.3689384601189545
1104	48-49	0.40891934449690837
1104	50-51	0.42643567971265384
1104	52-53	0.47573259499633025
1104	54-55	0.48701865800234145
1104	56-57	0.5454833085963671
1104	58-59	0.47377909761423354
1104	60-61	0.45128220730845214
1104	62-63	0.4690449349866057
1104	64-65	0.5180051041572113
1104	66-67	0.46155657378002246
1104	68-69	0.46014026106985995
1104	70-71	0.4564398512458041
1104	72-73	0.44937228462695344
1104	74-75	0.3775911954946096
1104	76-77	0.4413675159946422
1104	78-79	0.4500846391708748
1104	80-81	0.3939329813381427
1104	82-83	0.45851481850506204
1104	84-85	0.4994489790110883
1104	86-87	0.42766396692653785
1104	88-89	0.36625830153158745
1104	90-91	0.37147000164453203
1104	92-93	0.3814783138167819
1104	94-95	0.37583967672860297
1104	96-97	0.4630551485542256
1104	98-99	0.423838582308381
1104	100	0.42938429886869045
1105	1	1.57017333159747
1105	2	1.2427384743623406
1105	3	1.186956862598901
1105	4	0.6239113915365877
1105	5	0.9728651074882251
1105	6	0.6269662585783919
1105	7	0.3469926210550298
1105	8	0.26275843673175814
1105	9	0.15280540930173458
1105	10-11	0.20126131809325187
1105	12-13	0.20572799511783302
1105	14-15	0.26482477686498385
1105	16-17	0.2684764046976085
1105	18-19	0.29132669112334497
1105	20-21	0.3542368679789192
1105	22-23	0.35092337507466453
1105	24-25	0.4320273055433006
1105	26-27	0.39699233477831086
1105	28-29	0.4392025283251826
1105	30-31	0.41059706118853256
1105	32-33	0.4624644335741621
1105	34-35	0.448536910909624
1105	36-37	0.44509543976431587
1105	38-39	0.43381165158892543
1105	40-41	0.41699994160705245
1105	42-43	0.39284137524479945
1105	44-45	0.42227000809466375
1105	46-47	0.41490527786324094
1105	48-49	0.42995276607307176
1105	50-51	0.38718976054813936
1105	52-53	0.2908687177308167
1105	54-55	0.20923118089329762
1105	56-57	0.14466888105194897
1105	58-59	0.16292691647711166
1105	60-61	0.17983514088174246
1105	62-63	0.22559952166331243
1105	64-65	0.11556150524142339
1105	66-67	0.10486661817555643
1105	68-69	0.22319160743193578
1105	70-71	0.2235329722059518
1105	72-73	0.16840237258512758
1105	74-75	0.2512344224621259
1105	76-77	0.24341860827639294
1105	78-79	0.17238224206548836
1105	80-81	0.22440743677867658
1105	82-83	0.23732578591847187
1105	84-85	0.23574988600399038
1105	86-87	0.25076281892634356
1105	88-89	0.17483145610202655
1105	90-91	0.1795565028110815
1105	92-93	0.2097058980823583
1105	94-95	0.2531212071987383
1105	96-97	0.27100881105334196
1105	98-99	0.1766502813887385
1105	100	0.23291106006166373
1106	1	1.5885449193042014
1106	2	1.2776010337839239
1106	3	1.157861399039394
1106	4	0.6723102111701422
1106	5	0.9801693365020938
1106	6	0.6506893468876385
1106	7	0.3692981310131813
1106	8	0.27908810435043563
1106	9	0.2780210349244143
1106	10-11	0.21141493652367416
1106	12-13	0.1863895316816766
1106	14-15	0.27927857272990764
1106	16-17	0.3068459049395642
1106	18-19	0.30321290531620804
1106	20-21	0.3586566002141254
1106	22-23	0.3753210940449705
1106	24-25	0.3240788089923683
1106	26-27	0.27824405456462387
1106	28-29	0.4040704961782282
1106	30-31	0.4330515402237438
1106	32-33	0.4293724238200056
1106	34-35	0.38881928796269705
1106	36-37	0.4262370932261845
1106	38-39	0.39955350810418366
1106	40-41	0.3162864081933847
1106	42-43	0.36077412092516425
1106	44-45	0.3900537846979759
1106	46-47	0.3707781702354538
1106	48-49	0.40260318214275514
1106	50-51	0.35495266250372026
1106	52-53	0.32936915894497076
1106	54-55	0.34100998553505235
1106	56-57	0.17185241664796536
1106	58-59	0.16714264647303168
1106	60-61	0.15496869118489798
1106	62-63	0.16651383123434016
1106	64-65	0.20147111801671258
1106	66-67	0.21573282117201842
1106	68-69	0.20869036918578132
1106	70-71	0.23002214111031094
1106	72-73	0.22726603266995937
1106	74-75	0.2348686986337114
1106	76-77	0.26251132017778644
1106	78-79	0.23008166611498027
1106	80-81	0.1995409870818321
1106	82-83	0.2738943733690107
1106	84-85	0.22230851055447332
1106	86-87	0.2473222974396876
1106	88-89	0.24810146967055147
1106	90-91	0.18531031480641502
1106	92-93	0.15228733292973828
1106	94-95	0.18251460891235638
1106	96-97	0.18923142968301576
1106	98-99	0.1783743375225626
1106	100	0.18402073735858693
1107	1	1.6209942369901107
1107	2	1.2425398601281756
1107	3	1.2166623653405075
1107	4	0.6518951675704301
1107	5	0.9930080095643063
1107	6	0.6239559977109153
1107	7	0.4249560524172864
1107	8	0.3573441195817253
1107	9	0.3097702982967192
1107	10-11	0.2186531825068343
1107	12-13	0.14983273881517079
1107	14-15	0.26303027956829794
1107	16-17	0.27363600946952715
1107	18-19	0.25567064190156685
1107	20-21	0.33581641250488303
1107	22-23	0.35740046294609584
1107	24-25	0.3201472426858132
1107	26-27	0.28444349014351644
1107	28-29	0.3170786839948221
1107	30-31	0.342344689538983
1107	32-33	0.34903304451442096
1107	34-35	0.28753320062050847
1107	36-37	0.3178342154830105
1107	38-39	0.3418902285080563
1107	40-41	0.3334790413532218
1107	42-43	0.35708366776188427
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2216	90-91	-1.2239281274320604
2216	92-93	-1.238757160155611
2216	94-95	-1.2726446346047453
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2216	98-99	-1.3932215756762893
2216	100	-1.382089133302749
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7392.0
3	7581.0
4	9602.0
5	12726.0
6	9112.0
7	6725.0
8	6216.0
9	6645.0
10	6976.0
11	12322.0
12	8741.0
13	11408.0
14	10359.0
15	10970.0
16	13147.0
17	17705.0
18	23805.0
19	38975.0
20	89134.0
21	134406.0
22	60826.0
23	34180.0
24	31072.0
25	31712.0
26	32246.0
27	34782.0
28	40109.0
29	47672.0
30	56010.0
31	69108.0
32	88998.0
33	121192.0
34	179880.0
35	443714.0
36	542308.0
37	986555.0
38	1524616.0
39	729444.0
40	5918.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.059250851800524	44.41881610270177	26.4657840127621	16.056149032735608
2	16.384840984911946	41.73311030725312	25.452460799205856	16.42958790862907
3	17.727324587666924	26.394549122429638	21.085070403292853	34.79305588661059
4	32.677229513644065	27.173002879042258	23.086314402605545	17.063453204708136
5	16.45803683186443	40.69027631438613	24.20166042639523	18.65002642735421
6	35.32854748879419	24.28250483986303	24.44154448313751	15.94740318820527
7	17.93839313306405	24.552998579834743	40.27150463938213	17.237103647719078
8	34.43932540606062	24.68338780903401	24.03601991101848	16.84126687388689
9	34.14250232863863	26.57689303741137	23.601159023445174	15.679445610504828
10-11	15.930949483212093	43.85888895005331	23.85427073324093	16.355890833493664
12-13	15.891489345853751	26.077427983886746	32.450603520273006	25.58047914998649
14-15	16.212505100574425	34.64640801470564	23.678592412234693	25.462494472485243
16-17	25.40616864556917	25.70892442351363	32.347032663632234	16.537874267284963
18-19	16.56757272079761	25.91215013529905	32.40666800077794	25.113609143125405
20-21	25.568924524130182	34.89290805769828	23.449695319413642	16.088472098757897
22-23	25.166940286651034	26.10946900766557	32.37901683387417	16.344573871809224
24-25	16.40259772113469	34.748689829589274	23.475773770429257	25.37293867884678
26-27	16.781256946798212	29.726023502128363	37.166859151931696	16.325860399141735
28-29	24.919266592747718	25.809609596406542	32.98623948467242	16.28488432617332
30-31	22.591366774065165	27.13400805402441	33.42306575279074	16.85155941911968
32-33	18.784270813026637	28.5633515299492	34.48007380630243	18.17230385072174
34-35	18.745777182045206	28.310616586847303	34.68492280897762	18.258683422129874
36-37	18.73392763197946	28.31400848106752	35.01396096019423	17.938102926758795
38-39	19.303403869495938	28.030308468092002	34.67283806981135	17.99344959260071
40-41	18.309359915932554	29.23059719281008	34.50914872990426	17.9508941613531
42-43	18.39740353132676	28.246289883041904	35.01484482834742	18.341461757283923
44-45	18.333532889851526	28.77776386837555	34.41355184378413	18.4751513979888
46-47	18.984652237441345	28.25597984093121	34.63361991634604	18.1257480052814
48-49	18.472438967710662	28.47053663427249	34.58852818442351	18.468496213593344
50-51	18.990529600166433	29.019232874447365	33.90072455440426	18.08951297098194
52-53	18.992315663865888	28.746150201374082	33.8931256658571	18.36840846890293
54-55	19.19051754838616	29.569593941464195	32.347791643348764	18.89209686680088
56-57	19.553026645160735	30.204786326805095	31.282447969919552	18.95973905811462
58-59	20.536171293687726	30.48401975660557	30.136173256047837	18.843635693658868
60-61	20.12685659726528	30.947931471637368	29.895359659438547	19.029852271658807
62-63	19.389087473713374	31.034905004791774	29.858626782153706	19.71738073934114
64-65	19.826552394048658	30.91152387798161	30.20599015474336	19.055933573226365
66-67	19.839567470615894	30.35742576847803	30.76293115038601	19.040075610520066
68-69	20.15730617356421	30.48652835154725	30.398095407836657	18.95807006705188
70-71	19.057423980332096	31.267874616207614	30.583718726888083	19.090982676572203
72-73	19.043165144773774	30.41868088323385	30.964365740485878	19.573788231506498
74-75	19.30578886592401	30.78489822196983	30.270364259121695	19.63894865298446
76-77	19.959413820595206	30.325541566948484	30.507405369774286	19.207639242682024
78-79	19.745837079854713	30.666436992073226	30.011464650690655	19.576261277381406
80-81	20.106393397021233	31.123725180642747	29.694817362271543	19.075064060064477
82-83	20.073765880091653	30.869779818895843	29.956763978527984	19.099690322484523
84-85	19.844321638863413	31.381815334256796	29.290457405568986	19.483405621310805
86-87	19.820045472409934	31.54088346863674	29.460955887256183	19.17811517169714
88-89	20.75621406158348	31.106023734709705	29.011819382449804	19.12594282125701
90-91	20.206389493982137	31.695102415212446	28.9667496716506	19.131758419154817
92-93	19.598391122335812	31.844219314472603	28.882644801397404	19.67474476179418
94-95	19.93368070024256	31.979812421142267	28.809787320208464	19.276719558406704
96-97	19.803729149787934	31.374873471965603	29.28245272607387	19.538944652172592
98-99	20.38678250087152	31.554675441240025	28.55181835055913	19.506723707329325
100	19.684014511877663	32.36284636605241	28.55033494120131	19.402804180868614
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	193326.0
1	102921.0
2	9066.0
3	4522.0
4	3368.0
5	3080.5
6	3074.0
7	3670.0
8	3446.5
9	3011.5
10	3097.5
11	4663.0
12	47089.5
13	53604.0
14	17763.5
15	16885.5
16	18902.5
17	21235.0
18	23356.0
19	25707.5
20	28939.5
21	32830.5
22	37346.5
23	43459.0
24	50161.0
25	57555.5
26	67885.5
27	78455.0
28	86504.5
29	94621.0
30	103376.5
31	111527.5
32	121010.0
33	131525.5
34	142025.0
35	150580.5
36	157334.5
37	162564.0
38	165771.0
39	167735.0
40	168786.5
41	168894.5
42	167231.0
43	167386.5
44	168058.5
45	166029.5
46	163435.5
47	162394.0
48	159729.5
49	162827.0
50	196706.0
51	190905.0
52	140132.0
53	118877.5
54	110857.5
55	105339.0
56	94744.0
57	81374.5
58	73571.0
59	62236.5
60	48950.0
61	39204.5
62	30590.0
63	23519.0
64	17903.0
65	13369.5
66	10159.0
67	8214.0
68	6631.5
69	5180.5
70	3846.0
71	3016.5
72	2382.0
73	1635.0
74	1151.0
75	834.0
76	595.0
77	439.0
78	342.5
79	224.5
80	115.0
81	59.0
82	28.0
83	19.0
84	10.5
85	6.5
86	3.5
87	0.5
88	1.5
89	1.5
90	1.5
91	1.5
92	1.0
93	1.0
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.054521119803120806
2	0.0
3	1.8167650717467778E-5
4	5.086942200890978E-4
5	0.00948351367451818
6	1.8167650717467778E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.2717355502227446E-4
16-17	3.179338875556861E-4
18-19	9.083825358733889E-6
20-21	0.0
22-23	9.083825358733889E-6
24-25	0.00425123026788746
26-27	0.007348814715215716
28-29	0.0011263943444830024
30-31	0.006040743863558036
32-33	0.002761482909055102
34-35	0.026288590588175873
36-37	0.026234087636023473
38-39	0.02311833553797775
40-41	0.0024889681482930855
42-43	0.006395013052548657
44-45	0.007594077999901531
46-47	0.0012081487727116072
48-49	0.00948351367451818
50-51	0.010201135877858158
52-53	0.0019076033253341168
54-55	0.011790805315636588
56-57	0.006930958748713958
58-59	0.012935367310837057
60-61	0.007930179538174686
62-63	0.0011627296459179378
64-65	0.013171546770164138
66-67	0.005323121660218059
68-69	0.002152866610019932
70-71	0.009074741533375156
72-73	0.00523228340663072
74-75	0.008602382614720993
76-77	0.008084604569273162
78-79	0.015451586935206346
80-81	0.008538795837209855
82-83	0.011999733298887467
84-85	0.006849204320485352
86-87	0.013298720325186414
88-89	0.02043860705715125
90-91	0.018258488971055118
92-93	0.017250184356235657
94-95	0.0056410555477737455
96-97	0.010319225607521697
98-99	0.017504531466280206
100	0.016986753420832373
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5504289.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.183385890800515
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.89995722508992	41.667000573131006
2	15.476043861096848	16.7708891317753
3	4.350132831866524	7.071147777157942
4	1.3631789734353774	2.954466094234974
5	0.5390403567496972	1.460351583024181
6	0.2476464620198828	0.8050994289668869
7	0.1783195933488948	0.6763371536819655
8	0.13410730302405208	0.5813110200421386
9	0.10067364906288469	0.4909355258588378
>10	0.5643297301217478	5.4800552865440215
>50	0.12037163795159948	4.782293244137473
>100	0.021520671370950557	1.8718963286983568
>500	0.0019181970935888812	0.7151597136951765
>1k	0.002490287498043686	2.537491350977384
>5k	1.6826266878609822E-4	0.6822828887963117
>10k+	1.0095760127165893E-4	11.453282899277976
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	405330	7.363893865311215	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	186179	3.3824350429274332	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	36616	0.6652266986708001	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9350	0.16986753420832373	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA	8606	0.1563508020745277	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7654	0.1390551985914984	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	6183	0.11233058438610327	No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5625	0.10219303528575625	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.995324736764367E-4	0.0	0.0	0.0	0.0
2	5.995324736764367E-4	0.0	0.0	0.0	0.0
3	5.995324736764367E-4	0.0	0.0	0.0	0.0
4	5.995324736764367E-4	0.0	0.0	0.0	0.0
5	5.995324736764367E-4	0.0	0.0	0.0	1.8167650717467778E-5
6	5.995324736764367E-4	0.0	0.0	0.0	1.8167650717467778E-5
7	5.995324736764367E-4	0.0	0.0	0.0	1.8167650717467778E-5
8	5.995324736764367E-4	0.0	0.0	0.0	1.8167650717467778E-5
9	5.995324736764367E-4	0.0	0.0	0.0	1.8167650717467778E-5
10-11	6.177001243939045E-4	0.0	9.083825358733889E-6	0.0	1.8167650717467778E-5
12-13	6.903707272637756E-4	0.0	1.8167650717467778E-5	0.0	1.8167650717467778E-5
14-15	7.721251554923805E-4	0.0	1.8167650717467778E-5	0.0	1.8167650717467778E-5
16-17	8.629634090797195E-4	0.0	1.8167650717467778E-5	0.0	3.6335301434935555E-5
18-19	9.719693133845261E-4	0.0	1.8167650717467778E-5	0.0	3.6335301434935555E-5
20-21	0.001062807566971865	0.0	1.8167650717467778E-5	0.0	3.6335301434935555E-5
22-23	0.001217232598070341	0.0	1.8167650717467778E-5	0.0	3.6335301434935555E-5
24-25	0.0013262385023751478	0.0	1.8167650717467778E-5	0.0	4.541912679366944E-5
26-27	0.0014443282320386883	0.0	1.8167650717467778E-5	9.083825358733889E-6	5.4502952152403337E-5
28-29	0.0015715017870609627	0.0	1.8167650717467778E-5	1.8167650717467778E-5	7.267060286987111E-5
30-31	0.0017622621195943745	0.0	1.8167650717467778E-5	1.8167650717467778E-5	7.267060286987111E-5
32-33	0.0019076033253341168	0.0	1.8167650717467778E-5	1.8167650717467778E-5	7.267060286987111E-5
34-35	0.0020711121817913267	0.0	1.8167650717467778E-5	1.8167650717467778E-5	9.992207894607278E-5
36-37	0.002198285736813601	0.0	1.8167650717467778E-5	1.8167650717467778E-5	1.0900590430480667E-4
38-39	0.0025525549258042226	0.0	1.8167650717467778E-5	1.8167650717467778E-5	1.0900590430480667E-4
40-41	0.002888656464077377	0.0	1.8167650717467778E-5	1.8167650717467778E-5	1.0900590430480667E-4
42-43	0.0033428477320140712	0.0	1.8167650717467778E-5	1.8167650717467778E-5	1.0900590430480667E-4
44-45	0.003915128729614307	0.0	1.8167650717467778E-5	1.8167650717467778E-5	1.0900590430480667E-4
46-47	0.0045782479808018795	0.0	1.8167650717467778E-5	1.8167650717467778E-5	1.0900590430480667E-4
48-49	0.005168696629119583	0.0	1.8167650717467778E-5	1.8167650717467778E-5	1.0900590430480667E-4
50-51	0.006058911514275504	0.0	1.8167650717467778E-5	1.8167650717467778E-5	1.0900590430480667E-4
52-53	0.006794701368332949	0.0	1.8167650717467778E-5	1.8167650717467778E-5	1.0900590430480667E-4
54-55	0.0075759103491840635	0.0	1.8167650717467778E-5	1.8167650717467778E-5	1.0900590430480667E-4
56-57	0.00862055026543846	0.0	1.8167650717467778E-5	1.8167650717467778E-5	1.0900590430480667E-4
58-59	0.009492597499876913	0.0	1.8167650717467778E-5	2.7251476076201668E-5	1.0900590430480667E-4
60-61	0.011273027270188756	0.0	1.8167650717467778E-5	3.6335301434935555E-5	1.0900590430480667E-4
62-63	0.013516732133796026	0.0	1.8167650717467778E-5	3.6335301434935555E-5	1.0900590430480667E-4
64-65	0.015987532631371645	0.0	1.8167650717467778E-5	5.4502952152403337E-5	1.0900590430480667E-4
66-67	0.0190851170786999	0.0	1.8167650717467778E-5	5.4502952152403337E-5	1.0900590430480667E-4
68-69	0.02354527532983824	0.0	1.8167650717467778E-5	5.4502952152403337E-5	1.1808972966354057E-4
70-71	0.028995570545078574	0.0	1.8167650717467778E-5	5.4502952152403337E-5	1.3625738038100836E-4
72-73	0.03616270875311961	0.0	1.8167650717467778E-5	5.4502952152403337E-5	1.6350885645721E-4
74-75	0.04500127082716769	0.0	1.8167650717467778E-5	5.4502952152403337E-5	1.7259268181594388E-4
76-77	0.056782992317445546	0.0	1.8167650717467778E-5	5.4502952152403337E-5	1.8167650717467777E-4
78-79	0.07067216129094966	0.0	1.8167650717467778E-5	5.4502952152403337E-5	1.8167650717467777E-4
80-81	0.08903965616630959	0.0	1.8167650717467778E-5	5.4502952152403337E-5	1.8167650717467777E-4
82-83	0.11164929748419823	0.0	1.8167650717467778E-5	5.4502952152403337E-5	1.8167650717467777E-4
84-85	0.1414805799622803	0.0	1.8167650717467778E-5	6.358677751113723E-5	1.8167650717467777E-4
86-87	0.17802480938046678	0.0	1.8167650717467778E-5	7.267060286987111E-5	1.8167650717467777E-4
88	0.21021788645181966	0.0	1.8167650717467778E-5	7.267060286987111E-5	1.8167650717467777E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	2210	0.0	79.56868	1
AACGAGT	120215	0.0	72.52049	1
ACGAGTG	124860	0.0	71.3345	2
GTGGAAT	130225	0.0	70.43401	6
CGAGTGG	127820	0.0	70.307556	3
TGGAATC	131275	0.0	70.01024	7
GAATCAC	131400	0.0	69.94721	9
GGAATCA	131790	0.0	69.76875	8
AGTGGAA	130270	0.0	69.42536	5
GAGTGGA	131900	0.0	68.59948	4
AACGTGT	1980	0.0	52.479527	1
CGTGTGG	2415	0.0	50.98075	3
TCGAGTG	1375	0.0	49.55499	2
ACGTGTG	2365	0.0	49.276817	2
ATCGAGT	1365	0.0	42.02329	1
GAGTACT	58955	0.0	41.493275	20-21
AGTACTT	60340	0.0	41.35083	20-21
GTACTTT	59100	0.0	40.586422	22-23
CATGGGG	27915	0.0	39.19392	24-25
TACTTTT	63720	0.0	38.407063	22-23
>>END_MODULE