FastQCFastQC Report
Thu 26 May 2016
SRR2132700_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132700_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3666689
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2495596.806113090038451No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA553381.5092089893634284No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG234370.6391870158609034No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA51650.14086277838125894No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT47850.13049920514120505No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT46750.12749922341382103No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC42650.11631747333902603No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT14100.078.040341
AACGAGT737100.072.632171
ACGAGTG762400.071.73962
GTGGAAT791600.070.845656
CGAGTGG781200.070.362043
TGGAATC800400.070.278087
GGAATCA808000.069.750818
GAATCAC809100.069.609519
AGTGGAA795550.069.475865
GAGTGGA804200.068.805484
CGTGTGG14900.055.5076143
AACGTGT12150.054.5714151
ACGTGTG16250.047.4260142
ATCGAGT6500.042.6835981
GAGTACT356350.041.32291420-21
AGTACTT364150.041.2056320-21
GTACTTT358350.040.4579222-23
CGATTGG3800.039.572413
TACTTTT389000.038.0373122-23
TCGAGTG7800.037.3528062
Produced by FastQC (version 0.11.5)