FastQCFastQC Report
Thu 26 May 2016
SRR2132699_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132699_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3696991
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1952815.28216054623882No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA151100.40871075964209813No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53950.14592948698008731No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG50500.1365975735402115No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA43200.11685178568192349No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT37960.10267809686309759No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAGT479700.086.1373441
ACGAGTG505900.083.5484542
CGAGTGG517900.082.1933443
GTGGAAT542600.080.098446
GAATCAC547650.079.5818569
TGGAATC548450.079.362967
GAGTGGA540800.079.1560754
GGAATCA552100.078.914898
AGTGGAA545600.078.545825
TACGAGT12150.076.6318741
CGTGTGG13150.043.9571573
AGTACTT283700.040.5593520-21
GAGTACT276300.040.54857620-21
TGAGTAC554950.040.2468518-19
ATCACTG563500.040.19019710-11
GCTGAGT566450.039.84401316-17
CACTGCT569200.039.75084712-13
GTACTTT278550.039.50561522-23
AATCACT553750.039.38734410-11
AACGTGT12250.039.1547621
Produced by FastQC (version 0.11.5)