##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132699_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3696991
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.876660776290773	33.0	31.0	34.0	25.0	34.0
2	31.618105913701168	34.0	31.0	34.0	28.0	34.0
3	31.8258924622754	34.0	31.0	34.0	28.0	34.0
4	35.792109853662076	37.0	35.0	37.0	35.0	37.0
5	35.474095825497	37.0	35.0	37.0	33.0	37.0
6	35.83795822061779	37.0	36.0	37.0	35.0	37.0
7	36.049925195922846	37.0	36.0	37.0	35.0	37.0
8	36.180571713590865	37.0	37.0	37.0	35.0	37.0
9	38.120609706650626	39.0	39.0	39.0	37.0	39.0
10-11	38.1973500882204	39.0	39.0	39.0	37.0	39.0
12-13	38.19564140134504	39.0	39.0	39.0	37.0	39.0
14-15	39.73469464762019	41.0	40.0	41.0	38.0	41.0
16-17	39.69331112788752	41.0	40.0	41.0	38.0	41.0
18-19	39.691285696935694	41.0	40.0	41.0	37.0	41.0
20-21	39.60154311979662	41.0	40.0	41.0	37.0	41.0
22-23	39.52859041312246	41.0	39.5	41.0	37.0	41.0
24-25	39.49177777278874	41.0	39.5	41.0	37.0	41.0
26-27	39.41384209482793	41.0	39.0	41.0	37.0	41.0
28-29	39.26354486662261	41.0	39.0	41.0	36.0	41.0
30-31	39.177774844461354	41.0	39.0	41.0	36.0	41.0
32-33	39.03869701062296	41.0	39.0	41.0	35.5	41.0
34-35	38.93877182822463	41.0	39.0	41.0	35.0	41.0
36-37	38.81452024092025	40.5	39.0	41.0	35.0	41.0
38-39	38.44619976083253	40.0	38.0	41.0	35.0	41.0
40-41	38.218991065977704	40.0	37.0	41.0	35.0	41.0
42-43	38.08787403052915	40.0	37.0	41.0	34.0	41.0
44-45	37.912898760099765	40.0	36.5	41.0	34.0	41.0
46-47	37.79619736699386	40.0	36.0	41.0	34.0	41.0
48-49	37.64189688316796	40.0	35.5	41.0	33.0	41.0
50-51	37.42039161036638	40.0	35.0	41.0	33.0	41.0
52-53	37.04538934501058	40.0	35.0	41.0	33.0	41.0
54-55	36.362129634613666	40.0	35.0	41.0	30.5	41.0
56-57	35.81208231234537	39.0	35.0	41.0	28.5	41.0
58-59	35.511808927855114	39.0	35.0	41.0	28.0	41.0
60-61	35.294760522814364	39.0	35.0	41.0	27.0	41.0
62-63	35.03660287514901	38.5	35.0	41.0	27.0	41.0
64-65	34.773152139131525	38.0	35.0	40.5	26.0	41.0
66-67	34.51234598623583	37.0	35.0	40.0	26.0	41.0
68-69	34.2127926467768	37.0	35.0	40.0	26.0	41.0
70-71	33.87135240523983	36.0	34.0	39.0	25.5	41.0
72-73	33.529678324886376	36.0	34.0	39.0	25.0	41.0
74-75	33.183385623605794	35.0	34.0	39.0	24.5	40.5
76-77	32.815112208820636	35.0	34.0	37.5	24.0	39.5
78-79	32.48778439006208	35.0	34.0	37.0	23.0	39.0
80-81	32.166357180745095	35.0	33.0	36.5	22.5	39.0
82-83	31.861047673635127	35.0	33.0	36.0	20.5	38.0
84-85	31.55089598541084	35.0	33.0	36.0	20.0	37.0
86-87	31.433512415907963	35.0	33.0	35.5	20.0	37.0
88-89	31.568748341556688	35.0	34.0	35.0	22.0	36.5
90-91	31.48447954025314	35.0	34.0	35.0	20.5	36.0
92-93	31.357072008019493	35.0	34.0	35.0	20.0	36.0
94-95	31.234331108731396	35.0	34.0	35.0	19.0	36.0
96-97	31.1158699060939	35.0	34.0	35.0	16.5	36.0
98-99	30.99673775240459	35.0	34.0	35.0	4.5	35.5
100	30.908291635008037	35.0	34.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.897567370430071
1101	2	1.401864864056499
1101	3	1.3244606294160803
1101	4	0.6966904841636392
1101	5	0.9594831230959571
1101	6	0.6299695722777585
1101	7	0.4320086538941226
1101	8	0.3214846937707847
1101	9	0.337096909205556
1101	10-11	0.21090035393145
1101	12-13	0.2221367210772769
1101	14-15	0.30273183903486967
1101	16-17	0.2906499920906995
1101	18-19	0.2763372113950737
1101	20-21	0.3472103136544149
1101	22-23	0.37294161693436223
1101	24-25	0.3746399947827328
1101	26-27	0.3666987370117809
1101	28-29	0.39626749417126206
1101	30-31	0.3891312381954961
1101	32-33	0.3207369602386123
1101	34-35	0.3333057179343797
1101	36-37	0.3430757315223687
1101	38-39	0.374923178793658
1101	40-41	0.4102377233503134
1101	42-43	0.406166655869761
1101	44-45	0.4426983654897185
1101	46-47	0.376672384216441
1101	48-49	0.3996837903593402
1101	50-51	0.29688602933953234
1101	52-53	0.35475335505710603
1101	54-55	0.3989452637669899
1101	56-57	0.41877461447089814
1101	58-59	0.34042780666172234
1101	60-61	0.28476194364150587
1101	62-63	0.28311492171571473
1101	64-65	0.20801032550890852
1101	66-67	0.24494056066484404
1101	68-69	0.27644257250236137
1101	70-71	0.24845214476761157
1101	72-73	0.3076299914662215
1101	74-75	0.25357177878819215
1101	76-77	0.2914232693996368
1101	78-79	0.30490418871508496
1101	80-81	0.3057953887519105
1101	82-83	0.2663552312857682
1101	84-85	0.2737029050712856
1101	86-87	0.28349610575284956
1101	88-89	0.2783548107820373
1101	90-91	0.33040639811934014
1101	92-93	0.2966313839217989
1101	94-95	0.29822560496685213
1101	96-97	0.3377343226826639
1101	98-99	0.3427234386055318
1101	100	0.351596512024841
1102	1	1.3495702450707476
1102	2	1.2056097084509503
1102	3	1.1154414443142215
1102	4	0.5888242799391676
1102	5	0.8912244679278558
1102	6	0.5966627981245267
1102	7	0.3431541357088932
1102	8	0.2835941175928056
1102	9	0.29428976009919694
1102	10-11	0.23415057265410866
1102	12-13	0.21462359771770423
1102	14-15	0.3259656535580504
1102	16-17	0.30293361288810416
1102	18-19	0.2688358053208333
1102	20-21	0.361187472759525
1102	22-23	0.33187018836293447
1102	24-25	0.36028475793734316
1102	26-27	0.3025309767318163
1102	28-29	0.3536204812978738
1102	30-31	0.3673972361957496
1102	32-33	0.3991763740618879
1102	34-35	0.3389667290579865
1102	36-37	0.4296688136371074
1102	38-39	0.35598726453294205
1102	40-41	0.32105746463265206
1102	42-43	0.34891990546356055
1102	44-45	0.3301936785755828
1102	46-47	0.22447812724856675
1102	48-49	0.35045088197288266
1102	50-51	0.35269780286784425
1102	52-53	0.2421494617937654
1102	54-55	0.154682046669123
1102	56-57	0.16706857772549455
1102	58-59	0.1740165768154469
1102	60-61	0.19086664930330244
1102	62-63	0.1530046230030564
1102	64-65	0.09682031800984703
1102	66-67	0.058995053853195145
1102	68-69	0.10869479097506485
1102	70-71	0.051492389736985444
1102	72-73	0.14560102633685545
1102	74-75	0.10065964280519069
1102	76-77	0.028031193409127297
1102	78-79	0.09263619096480369
1102	80-81	0.09452179795076177
1102	82-83	0.0250353962651495
1102	84-85	0.06175049287281098
1102	86-87	-0.002570479673970283
1102	88-89	0.011362403582932501
1102	90-91	0.021308316629525592
1102	92-93	0.011148319304876253
1102	94-95	-0.024758240802427167
1102	96-97	-0.027386443471566935
1102	98-99	0.03711242123270253
1102	100	0.09325536716794858
1103	1	1.8878179184272845
1103	2	1.3771373319420306
1103	3	1.2612278744902312
1103	4	0.688881983165615
1103	5	0.9546304394403577
1103	6	0.6143758372022603
1103	7	0.4451740572078151
1103	8	0.36140491272475117
1103	9	0.34646643688707
1103	10-11	0.24181214168815757
1103	12-13	0.2563097045087872
1103	14-15	0.3368186430171889
1103	16-17	0.3023811122092397
1103	18-19	0.31657658514753706
1103	20-21	0.4143153270635125
1103	22-23	0.4091585687228516
1103	24-25	0.38775442517178504
1103	26-27	0.371296096828587
1103	28-29	0.4224932935124386
1103	30-31	0.4721722553273011
1103	32-33	0.42949756811753304
1103	34-35	0.3828293583502358
1103	36-37	0.4156094379428197
1103	38-39	0.4394642387855825
1103	40-41	0.3629689842949446
1103	42-43	0.41340572960140776
1103	44-45	0.3908005807454771
1103	46-47	0.37552434537293067
1103	48-49	0.3700792361657861
1103	50-51	0.38316788359064446
1103	52-53	0.42247065584941623
1103	54-55	0.4205313023900743
1103	56-57	0.3281753688089566
1103	58-59	0.23878320275042597
1103	60-61	0.25759857924479235
1103	62-63	0.25761131534302706
1103	64-65	0.17547459854009873
1103	66-67	0.27218872686412965
1103	68-69	0.348549514616721
1103	70-71	0.3141565507876152
1103	72-73	0.3162422727265479
1103	74-75	0.27738271669000625
1103	76-77	0.311931376084587
1103	78-79	0.251974393586444
1103	80-81	0.3501208623561993
1103	82-83	0.3286225620801524
1103	84-85	0.3087892682066844
1103	86-87	0.27430888663968744
1103	88-89	0.2227612593927546
1103	90-91	0.2573051012946621
1103	92-93	0.2707440264316894
1103	94-95	0.22198954981200458
1103	96-97	0.1811312624703234
1103	98-99	0.1541050957001069
1103	100	0.19947034408665942
1104	1	1.8999172323849614
1104	2	1.39594078215719
1104	3	1.2552282079809771
1104	4	0.6621692120902196
1104	5	0.9906665896019362
1104	6	0.5920521741186562
1104	7	0.35976735610597643
1104	8	0.30488463828425694
1104	9	0.2363621974595702
1104	10-11	0.2083476007107734
1104	12-13	0.20580248260751688
1104	14-15	0.2729104400285607
1104	16-17	0.2722682895193813
1104	18-19	0.308640252862304
1104	20-21	0.3209267774919624
1104	22-23	0.32320208293171504
1104	24-25	0.3342238570031242
1104	26-27	0.2966722019217727
1104	28-29	0.3199071419809627
1104	30-31	0.35158546553812897
1104	32-33	0.27777619831311284
1104	34-35	0.35691687669296357
1104	36-37	0.41326706682358605
1104	38-39	0.38288616689239774
1104	40-41	0.3406209980399595
1104	42-43	0.3138442500514316
1104	44-45	0.2777982934467289
1104	46-47	0.32760651932017737
1104	48-49	0.27100950448737393
1104	50-51	0.29248900159776525
1104	52-53	0.35892886551080494
1104	54-55	0.24024208239018208
1104	56-57	0.20097335767119517
1104	58-59	0.14823613724807672
1104	60-61	0.09518874989955606
1104	62-63	0.07959354075743619
1104	64-65	0.11206000310532716
1104	66-67	0.11292647424606628
1104	68-69	0.10619164884556653
1104	70-71	0.10553092818071264
1104	72-73	0.0844772677786878
1104	74-75	0.04450403823842208
1104	76-77	0.07558796221756126
1104	78-79	0.12958126308343765
1104	80-81	0.06428876169149333
1104	82-83	0.11450896178531522
1104	84-85	0.14120523192572065
1104	86-87	0.08755152590669368
1104	88-89	0.018848771401515307
1104	90-91	0.01208859082166569
1104	92-93	0.01728678498350078
1104	94-95	-0.04914426644461045
1104	96-97	0.0113492290193129
1104	98-99	-0.004453460502954698
1104	100	0.0052185741846706435
1105	1	1.5981248052099808
1105	2	1.3027613723459552
1105	3	1.2073574866104693
1105	4	0.6121375112274308
1105	5	0.9574450599323399
1105	6	0.5785570247098875
1105	7	0.3300436981429513
1105	8	0.2733540702658246
1105	9	0.2393876327693576
1105	10-11	0.21068184482000163
1105	12-13	0.1680720893937533
1105	14-15	0.20735438598180167
1105	16-17	0.168603782883757
1105	18-19	0.20346612290035893
1105	20-21	0.25802311432620684
1105	22-23	0.24413012613451457
1105	24-25	0.2732045688082394
1105	26-27	0.19783258936585923
1105	28-29	0.3035171770521501
1105	30-31	0.2901154901640055
1105	32-33	0.288854108029426
1105	34-35	0.2541668330700446
1105	36-37	0.23123022396917037
1105	38-39	0.22237203317315135
1105	40-41	0.15843642767111277
1105	42-43	0.1839680594729387
1105	44-45	0.253981744308831
1105	46-47	0.2256656604042675
1105	48-49	0.22074053619982692
1105	50-51	0.19292119038281186
1105	52-53	0.14551476617062065
1105	54-55	0.11231039278789723
1105	56-57	0.14205293121153773
1105	58-59	0.025582234058447284
1105	60-61	0.02244567313965007
1105	62-63	0.06692055800893115
1105	64-65	-0.01477497820101803
1105	66-67	0.04316942456530626
1105	68-69	0.10054794918664811
1105	70-71	0.06872337400702122
1105	72-73	0.03071951515183713
1105	74-75	0.11780188898892874
1105	76-77	0.16172476909951428
1105	78-79	0.07134877344428503
1105	80-81	0.10413583570505835
1105	82-83	0.08585384305806087
1105	84-85	0.06263962465520123
1105	86-87	0.02106086014446973
1105	88-89	-0.04089102970926817
1105	90-91	-0.016260118242232835
1105	92-93	0.027669324377328053
1105	94-95	0.01955160636838471
1105	96-97	0.06566020847247245
1105	98-99	0.0028894345665158028
1105	100	0.023506411759022683
1106	1	1.645251070957226
1106	2	1.3165825937490325
1106	3	1.1528996195294638
1106	4	0.6448373578352786
1106	5	0.9765995648389421
1106	6	0.6339679558345637
1106	7	0.389484775934136
1106	8	0.28119123270277413
1106	9	0.27225013876651616
1106	10-11	0.20397654074486127
1106	12-13	0.1865418251629194
1106	14-15	0.22702938024838915
1106	16-17	0.23441670955435967
1106	18-19	0.2417815453134935
1106	20-21	0.2593760005271051
1106	22-23	0.26514690521934625
1106	24-25	0.3119896279744765
1106	26-27	0.2960662421661766
1106	28-29	0.3810883986276181
1106	30-31	0.33427634216547375
1106	32-33	0.2860671093620013
1106	34-35	0.30574637364932755
1106	36-37	0.3103954937693061
1106	38-39	0.33403511820740306
1106	40-41	0.3378564914413218
1106	42-43	0.34669936643389576
1106	44-45	0.3988067279084291
1106	46-47	0.4134728532452172
1106	48-49	0.39570835942830485
1106	50-51	0.33763322948065166
1106	52-53	0.44145297711592235
1106	54-55	0.33846831181786285
1106	56-57	0.30318070895330607
1106	58-59	0.2652846328810625
1106	60-61	0.2415376310636148
1106	62-63	0.3053911860258651
1106	64-65	0.20546504196626358
1106	66-67	0.14934066939160573
1106	68-69	0.23559271809791937
1106	70-71	0.28697510896006406
1106	72-73	0.3161780553489777
1106	74-75	0.292852179631403
1106	76-77	0.29394144354174756
1106	78-79	0.26277712008709386
1106	80-81	0.22691509374380558
1106	82-83	0.2783650571783589
1106	84-85	0.2684875985977051
1106	86-87	0.2319163506194144
1106	88-89	0.2890272034360386
1106	90-91	0.310798819343038
1106	92-93	0.30140773498202833
1106	94-95	0.3059924120722606
1106	96-97	0.28073088015025505
1106	98-99	0.2964294020953133
1106	100	0.35907176656403905
1107	1	1.6194934219334236
1107	2	1.2859753244482448
1107	3	1.165771190684893
1107	4	0.6338286577282446
1107	5	0.97235125434824
1107	6	0.6335626173641984
1107	7	0.38390570323981876
1107	8	0.3067293206657311
1107	9	0.272523675254817
1107	10-11	0.16980695857740358
1107	12-13	0.08965325370820665
1107	14-15	0.19115199203175592
1107	16-17	0.1990970456396184
1107	18-19	0.15483288208748291
1107	20-21	0.23906869345658777
1107	22-23	0.2450946418453981
1107	24-25	0.2592895170177272
1107	26-27	0.20499489527669823
1107	28-29	0.21334093828256329
1107	30-31	0.23824716276187274
1107	32-33	0.23452384341736376
1107	34-35	0.2723759676051216
1107	36-37	0.27318368399806303
1107	38-39	0.2596473080283985
1107	40-41	0.27252861359045966
1107	42-43	0.28420382626794805
1107	44-45	0.32264834727580194
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2213	100	3.9392834953133615E-5
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2216	3	-5.596758703697827
2216	4	-3.0217336999516036
2216	5	-3.9965179655943217
2216	6	-4.369357591810658
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2216	14-15	-1.1215535497288371
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2216	54-55	-1.1456739478187004
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2216	64-65	-1.008415289094728
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2216	80-81	-0.9843029098530458
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2216	84-85	-1.022624347631453
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2216	90-91	-0.823396105868305
2216	92-93	-0.8318995804552429
2216	94-95	-0.8259464840525688
2216	96-97	-0.9133192383472917
2216	98-99	-0.9982385331897952
2216	100	-0.9807793646240164
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3030.0
3	968.0
4	1177.0
5	1488.0
6	1372.0
7	1452.0
8	1792.0
9	2150.0
10	2831.0
11	5577.0
12	4399.0
13	5928.0
14	5477.0
15	6002.0
16	6941.0
17	8814.0
18	11964.0
19	20233.0
20	46046.0
21	69787.0
22	34053.0
23	21067.0
24	19284.0
25	19535.0
26	20453.0
27	21766.0
28	25283.0
29	30496.0
30	36808.0
31	47145.0
32	61995.0
33	86083.0
34	130348.0
35	241221.0
36	435052.0
37	695374.0
38	1096162.0
39	464899.0
40	2539.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.376263216052468	37.299287990120796	27.046794981262686	20.277653812564054
2	19.52804321135756	34.02642851984222	26.69143636000196	19.754091908798262
3	20.98177165748352	24.534391491456564	21.114798795777105	33.369038055282815
4	30.450833329726784	25.311984713993276	23.355872475233813	20.88130948104613
5	19.521749504014682	33.082640053972725	24.489799230769645	22.905811211242945
6	33.77945301447935	22.151696380028078	24.72197652685022	19.346874078642355
7	21.42404458112016	22.44636246071467	35.29151139399582	20.838081564169347
8	32.4277500269814	22.441872322653747	24.599654151173212	20.530723499191637
9	32.38439044076656	24.52908324634818	23.99007733586584	19.09644897701942
10-11	19.28335773606157	36.85597287091043	24.182233605653895	19.67843578737411
12-13	19.211637247696846	24.167153774515544	30.357850478943554	26.26335849884406
14-15	19.47491522819682	30.25038788374524	24.10988254209377	26.16481434596417
16-17	25.999519066653896	23.873201301170848	30.323970010100144	19.803309622075112
18-19	19.723715854325853	23.959782428466823	30.424566897782547	25.891934819424772
20-21	26.11922777199079	30.384358522917694	24.009620256040655	19.48679344905086
22-23	25.79502514207504	24.116172886591524	30.44399633215129	19.644805639182145
24-25	19.54690332226174	30.301115896267333	24.041284888833705	26.110695892637214
26-27	19.857238405100734	26.761638180848035	33.76731685973183	19.613806554319403
28-29	25.916401053463417	23.80606600281286	30.689121041312855	19.588411902410865
30-31	24.212560511924934	24.898425445629616	30.928423887521937	19.960590154923512
32-33	21.38954634841718	25.755675716615855	31.999166867285744	20.855611067681213
34-35	21.243337149115558	25.585306812429366	32.10565432909106	21.065701709364014
36-37	21.179662383499174	25.77428787295652	32.12358032849868	20.922469415045626
38-39	21.450147869640958	25.57975694240592	32.001703930104775	20.968391257848346
40-41	21.233231044888033	25.959124925934535	31.97147554903117	20.836168480146263
42-43	21.325672776140912	25.693348142649857	32.03217563863825	20.94880344257098
44-45	21.244108976429413	25.806281017310994	31.9165796978191	21.033030308440495
46-47	21.404082076483753	25.727209136632386	31.89849881334357	20.970209973540292
48-49	21.259599056173116	25.904017215575443	31.862678463826395	20.973705264425043
50-51	21.422096413838887	25.92292920005183	31.57767878663441	21.077295599474873
52-53	21.44859492659606	26.054094146010996	31.308906514135536	21.18840441325741
54-55	21.80195750916623	26.45711833258016	30.365603609060308	21.3753205491933
56-57	22.098835580744144	26.826574419511484	29.458261214522924	21.616328785221448
58-59	22.402109100874153	27.200473197789886	28.888397740434563	21.509019960901394
60-61	22.270881157441245	27.335903910129723	28.734040521480473	21.65917441094856
62-63	22.042241882288277	27.469273971198398	28.67348382327364	21.81500032323969
64-65	22.0882272220711	27.427522788851423	28.78886846560771	21.695381523469766
66-67	22.08586857743608	27.212193354767184	28.985659355975525	21.716278711821207
68-69	22.153109031869686	27.190282599178317	28.954956897163836	21.701651471788164
70-71	21.87795366542609	27.411962848806414	28.977719996169533	21.732363489597958
72-73	21.833438266499584	27.235302988600267	29.06088310219859	21.870375642701553
74-75	21.970815191508823	27.25954223562823	28.84116262590509	21.92847994695786
76-77	22.086284218512098	27.272678088831352	28.91718468477912	21.723853007877427
78-79	22.071856012033265	27.43197472148728	28.59762959836815	21.898539668111308
80-81	22.086955275027716	27.525900253254132	28.521306403959006	21.865838067759142
82-83	22.127956919725765	27.524258489220056	28.50958504179989	21.838199549254288
84-85	22.098954170731634	27.725356845635122	28.263751370636463	21.91193761299678
86-87	22.154296556129953	27.848577765411807	28.175966370573224	21.821159307885015
88-89	22.337640659033546	27.79084052776576	27.98437393896156	21.88714487423913
90-91	22.23660888988748	28.045407822123796	27.919674779547837	21.798308508440886
92-93	22.11420283722902	28.06707043401969	27.860177315315937	21.958549413435353
94-95	22.11318597818698	28.238268245661157	27.731719714140645	21.916826062011214
96-97	22.03335062384481	28.061661525726457	27.856190735757476	22.04879711467126
98-99	22.190011925219565	28.210489323573846	27.51406245265623	22.085436298550363
100	22.16114773386084	28.385966117816892	27.486901434018048	21.965984714304227
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15671.0
1	8558.0
2	1160.5
3	761.0
4	657.0
5	618.5
6	619.5
7	716.0
8	699.5
9	714.0
10	725.5
11	1044.5
12	11129.0
13	14078.5
14	7462.0
15	8279.5
16	9632.5
17	11069.0
18	12420.0
19	13853.5
20	15484.0
21	17300.5
22	19552.5
23	22801.5
24	26447.5
25	30357.0
26	35593.0
27	41089.5
28	45466.0
29	49654.0
30	54777.0
31	60256.0
32	66249.5
33	72807.0
34	80155.0
35	86664.0
36	92034.5
37	97030.5
38	101581.0
39	105671.5
40	110036.0
41	114769.0
42	117997.5
43	120416.0
44	122566.5
45	123754.5
46	123870.5
47	123579.0
48	121868.0
49	119482.5
50	119982.0
51	113617.5
52	100672.0
53	92386.0
54	86544.0
55	80117.0
56	72766.0
57	66129.0
58	62622.0
59	57467.5
60	50465.0
61	45731.0
62	41294.5
63	37465.0
64	34025.0
65	30990.0
66	28102.5
67	25234.5
68	22375.5
69	19516.0
70	16850.5
71	14091.5
72	11630.0
73	9526.0
74	7490.0
75	5621.0
76	4154.5
77	3019.5
78	2086.5
79	1398.0
80	896.5
81	555.5
82	331.5
83	189.0
84	115.5
85	71.5
86	43.0
87	26.5
88	16.5
89	10.5
90	8.5
91	6.0
92	2.5
93	1.0
94	1.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05296198989935329
2	0.0
3	2.7049024463408216E-5
4	4.0573536695112325E-4
5	0.008358148559193139
6	2.7049024463408216E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.893431712438575E-4
16-17	3.516373180243068E-4
18-19	0.0
20-21	0.0
22-23	2.7049024463408216E-5
24-25	0.0038409614738039665
26-27	0.007262663068425106
28-29	0.0011495835396948492
30-31	0.005382755868218235
32-33	0.0027319514708042296
34-35	0.025371984946676907
36-37	0.025250264336591572
38-39	0.022450690304628818
40-41	0.00250203476286526
42-43	0.005923736357486399
44-45	0.007167991482803177
46-47	0.0013254021987070025
48-49	0.009156094780863681
50-51	0.00984584490468059
52-53	0.0022856425671579942
54-55	0.01096837941991203
56-57	0.006911025750400799
58-59	0.01188804625166791
60-61	0.007708971972071341
62-63	0.0011901570763899615
64-65	0.01356508576839922
66-67	0.005152839160279265
68-69	0.0020692503714507286
70-71	0.008466344657046772
72-73	0.0052339862336694895
74-75	0.008317575022498027
76-77	0.007830692582156679
78-79	0.01544499296860609
80-81	0.008358148559193139
82-83	0.012172061008533696
84-85	0.007019221848254432
86-87	0.012618369912179933
88-89	0.019745787858287997
90-91	0.018055223829324982
92-93	0.017257277607654442
94-95	0.0056261970883889085
96-97	0.009697075270131845
98-99	0.01685154224070332
100	0.01606712053126448
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3696991.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.75421354479086
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.29954475800352	74.41031816261584
2	4.874527810003344	7.775282637780569
3	0.5950006792726541	1.4236143370202061
4	0.23197946758597032	0.7400535998343346
5	0.15881068692045086	0.6332910718924281
6	0.1377820405477671	0.659321897069019
7	0.12001638538119515	0.6700268699996026
8	0.10702722209032173	0.6828697540557821
9	0.093385246232797	0.6703080182985031
>10	0.3682185215198952	4.35171754684656
>50	0.007818768466094746	0.3996291756257477
>100	0.005107270530417818	0.9039791551663543
>500	4.075531067422244E-4	0.22546994981814758
>1k	2.3773930664773674E-4	0.47239258192246353
>5k	6.792551618506764E-5	0.2829209012717966
>10k+	6.792551618506764E-5	5.698804340782628
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	195281	5.28216054623882	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15110	0.40871075964209813	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5395	0.14592948698008731	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	5050	0.1365975735402115	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA	4320	0.11685178568192349	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3796	0.10267809686309759	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.8688244034134786E-4	0.0	0.0	2.7049024463408216E-5	0.0
2	4.8688244034134786E-4	0.0	0.0	5.409804892681643E-5	0.0
3	4.8688244034134786E-4	0.0	0.0	5.409804892681643E-5	0.0
4	4.8688244034134786E-4	0.0	0.0	8.114707339022464E-5	2.7049024463408216E-5
5	4.8688244034134786E-4	0.0	0.0	8.114707339022464E-5	2.7049024463408216E-5
6	4.8688244034134786E-4	2.7049024463408216E-5	0.0	1.0819609785363286E-4	2.7049024463408216E-5
7	4.8688244034134786E-4	2.7049024463408216E-5	0.0	1.3524512231704108E-4	2.7049024463408216E-5
8	4.8688244034134786E-4	2.7049024463408216E-5	2.7049024463408216E-5	1.3524512231704108E-4	2.7049024463408216E-5
9	5.139314648047561E-4	2.7049024463408216E-5	2.7049024463408216E-5	1.3524512231704108E-4	5.409804892681643E-5
10-11	5.139314648047561E-4	2.7049024463408216E-5	2.7049024463408216E-5	1.4876963454874518E-4	6.762256115852054E-5
12-13	5.409804892681643E-4	4.057353669511232E-5	2.7049024463408216E-5	1.893431712438575E-4	8.114707339022464E-5
14-15	5.409804892681643E-4	6.762256115852054E-5	2.7049024463408216E-5	2.5696573240237806E-4	9.467158562192875E-5
16-17	5.409804892681643E-4	8.114707339022464E-5	2.7049024463408216E-5	2.7049024463408216E-4	1.2172061008533698E-4
18-19	5.950785381949807E-4	8.114707339022464E-5	2.7049024463408216E-5	2.9753926909749037E-4	1.3524512231704108E-4
20-21	6.491765871217971E-4	8.114707339022464E-5	4.057353669511232E-5	3.245882935608986E-4	1.3524512231704108E-4
22-23	6.627010993535013E-4	8.114707339022464E-5	5.409804892681643E-5	3.516373180243068E-4	1.622941467804493E-4
24-25	7.032746360486136E-4	8.114707339022464E-5	5.409804892681643E-5	3.651618302560109E-4	2.2991670793896983E-4
26-27	8.114707339022465E-4	8.114707339022464E-5	5.409804892681643E-5	4.0573536695112325E-4	2.7049024463408216E-4
28-29	9.872893929144E-4	8.114707339022464E-5	5.409804892681643E-5	4.3278439141453145E-4	2.7049024463408216E-4
30-31	0.001257779637548482	1.4876963454874518E-4	5.409804892681643E-5	4.4630890364623555E-4	2.8401475686578627E-4
32-33	0.0015823679311093805	1.622941467804493E-4	5.409804892681643E-5	5.00406952573052E-4	3.245882935608986E-4
34-35	0.002245069030462882	1.622941467804493E-4	5.409804892681643E-5	5.274559770364602E-4	3.245882935608986E-4
36-37	0.002677853421877413	1.622941467804493E-4	5.409804892681643E-5	5.545050014998684E-4	3.245882935608986E-4
38-39	0.003286456472304098	2.1639219570726573E-4	5.409804892681643E-5	5.680295137315725E-4	3.245882935608986E-4
40-41	0.004206123304059978	2.1639219570726573E-4	8.114707339022464E-5	5.680295137315725E-4	3.381128057926027E-4
42-43	0.005247510745901194	2.1639219570726573E-4	8.114707339022464E-5	6.086030504266848E-4	3.78686342487715E-4
44-45	0.006302422699974114	2.1639219570726573E-4	8.114707339022464E-5	6.491765871217971E-4	3.78686342487715E-4
46-47	0.007465530751900667	2.4344122017067393E-4	8.114707339022464E-5	7.438481727437259E-4	3.922108547194191E-4
48-49	0.009034374170778344	2.8401475686578627E-4	8.114707339022464E-5	7.708971972071341E-4	4.0573536695112325E-4
50-51	0.01129296771347293	3.381128057926027E-4	8.114707339022464E-5	7.844217094388383E-4	4.0573536695112325E-4
52-53	0.013659757354021149	3.516373180243068E-4	8.114707339022464E-5	7.844217094388383E-4	4.0573536695112325E-4
54-55	0.016310561751435155	3.651618302560109E-4	8.114707339022464E-5	7.979462216705424E-4	4.0573536695112325E-4
56-57	0.019137184807861314	3.922108547194191E-4	1.0819609785363286E-4	8.114707339022465E-4	4.0573536695112325E-4
58-59	0.022044954937677694	4.0573536695112325E-4	1.2172061008533698E-4	8.114707339022465E-4	4.0573536695112325E-4
60-61	0.025899440923713365	4.0573536695112325E-4	1.3524512231704108E-4	8.520442705973588E-4	4.1925987918282735E-4
62-63	0.030078515203309935	4.1925987918282735E-4	1.3524512231704108E-4	8.926178072924711E-4	4.4630890364623555E-4
64-65	0.03496086411895512	4.3278439141453145E-4	1.3524512231704108E-4	9.331913439875834E-4	5.00406952573052E-4
66-67	0.04015427681592949	4.3278439141453145E-4	1.3524512231704108E-4	9.872893929144E-4	5.139314648047561E-4
68-69	0.04638907695474509	4.3278439141453145E-4	1.3524512231704108E-4	0.001000813905146104	5.139314648047561E-4
70-71	0.05344887233969463	4.3278439141453145E-4	1.3524512231704108E-4	0.0010413874418412163	5.139314648047561E-4
72-73	0.06160415321541221	4.3278439141453145E-4	1.3524512231704108E-4	0.0010549119540729204	5.139314648047561E-4
74-75	0.07155819421794643	4.3278439141453145E-4	1.3524512231704108E-4	0.0010549119540729204	5.139314648047561E-4
76-77	0.08222903436876097	4.3278439141453145E-4	1.3524512231704108E-4	0.0010549119540729204	5.139314648047561E-4
78-79	0.09413060513266058	4.3278439141453145E-4	1.758186590121534E-4	0.0010819609785363287	5.139314648047561E-4
80-81	0.10953502456457156	4.3278439141453145E-4	1.893431712438575E-4	0.0011090100029997369	5.139314648047561E-4
82-83	0.12660295900098215	4.3278439141453145E-4	1.893431712438575E-4	0.0011090100029997369	5.139314648047561E-4
84-85	0.14514506527064847	4.5983341587793966E-4	1.893431712438575E-4	0.001122534515231441	5.139314648047561E-4
86-87	0.16687895642699696	4.7335792810964376E-4	1.893431712438575E-4	0.0011631080519265533	5.409804892681643E-4
88	0.18620548440610216	4.8688244034134786E-4	1.893431712438575E-4	0.0011901570763899615	5.409804892681643E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGAGT	47970	0.0	86.137344	1
ACGAGTG	50590	0.0	83.548454	2
CGAGTGG	51790	0.0	82.193344	3
GTGGAAT	54260	0.0	80.09844	6
GAATCAC	54765	0.0	79.581856	9
TGGAATC	54845	0.0	79.36296	7
GAGTGGA	54080	0.0	79.156075	4
GGAATCA	55210	0.0	78.91489	8
AGTGGAA	54560	0.0	78.54582	5
TACGAGT	1215	0.0	76.631874	1
CGTGTGG	1315	0.0	43.957157	3
AGTACTT	28370	0.0	40.55935	20-21
GAGTACT	27630	0.0	40.548576	20-21
TGAGTAC	55495	0.0	40.24685	18-19
ATCACTG	56350	0.0	40.190197	10-11
GCTGAGT	56645	0.0	39.844013	16-17
CACTGCT	56920	0.0	39.750847	12-13
GTACTTT	27855	0.0	39.505615	22-23
AATCACT	55375	0.0	39.387344	10-11
AACGTGT	1225	0.0	39.154762	1
>>END_MODULE