FastQCFastQC Report
Thu 26 May 2016
SRR2132698_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132698_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5323990
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3936937.394698337149394No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3066495.759759128022404No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG511800.9613090933679439No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT117430.22056765696404387No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78160.14680718784220106No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT74940.14075909233488418No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA71130.13360280541473596No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT67850.12744201247560571No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC65210.12248332547581794No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64640.12141269987359105No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT60380.11341118221484263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT22350.075.73291
AACGAGT1337700.064.4052351
GTGGAAT1427150.063.364026
ACGAGTG1392000.063.161242
TGGAATC1440700.063.018627
GAATCAC1448600.062.6911629
GGAATCA1452350.062.5422368
AGTGGAA1429500.062.2933165
CGAGTGG1429750.061.973463
GAGTGGA1465050.060.8330654
ACGTGTG23300.054.4561772
CGTGTGG24400.054.312353
AACGTGT21250.053.3233951
TCGAGTG14300.046.9936682
ATCGAGT13050.046.8373681
AGTACTT571900.041.21761720-21
GAGTACT565500.040.57467720-21
GTACTTT569950.039.4140322-23
TACTTTT613350.037.86640522-23
CATGGGG260650.037.44071624-25