##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132698_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5323990
Sequences flagged as poor quality	0
Sequence length	100
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.885660003117962	33.0	31.0	34.0	25.0	34.0
2	31.629282737195226	34.0	31.0	34.0	28.0	34.0
3	31.82941046095128	34.0	31.0	34.0	28.0	34.0
4	35.75887708278941	37.0	35.0	37.0	35.0	37.0
5	35.39152665576006	37.0	35.0	37.0	33.0	37.0
6	35.71125302639562	37.0	35.0	37.0	35.0	37.0
7	35.8873591047316	37.0	35.0	37.0	35.0	37.0
8	35.995810097314234	37.0	37.0	37.0	35.0	37.0
9	37.881849139461195	39.0	39.0	39.0	37.0	39.0
10-11	37.91142751958587	39.0	39.0	39.0	37.0	39.0
12-13	37.84071119592636	39.0	39.0	39.0	37.0	39.0
14-15	39.3282712777447	41.0	40.0	41.0	37.0	41.0
16-17	39.18757839515101	41.0	39.5	41.0	37.0	41.0
18-19	38.9195352545741	41.0	39.0	41.0	36.0	41.0
20-21	38.72775155475499	41.0	39.0	41.0	35.0	41.0
22-23	38.60236542517923	40.5	39.0	41.0	35.0	41.0
24-25	38.51133050587999	41.0	39.0	41.0	35.0	41.0
26-27	38.42875822456466	41.0	39.0	41.0	35.0	41.0
28-29	38.2802957931927	41.0	38.0	41.0	35.0	41.0
30-31	38.172780658866756	41.0	38.0	41.0	35.0	41.0
32-33	38.030840685275514	41.0	38.0	41.0	35.0	41.0
34-35	37.94255013626998	41.0	38.0	41.0	34.5	41.0
36-37	37.79340635500818	40.0	38.0	41.0	34.0	41.0
38-39	37.36400378287713	40.0	36.0	41.0	34.0	41.0
40-41	37.165174145706516	40.0	35.0	41.0	33.0	41.0
42-43	37.06107130178682	40.0	35.0	41.0	33.0	41.0
44-45	36.911429397876404	40.0	35.0	41.0	33.0	41.0
46-47	36.81448011735559	40.0	35.0	41.0	33.0	41.0
48-49	36.66147729428492	40.0	35.0	41.0	33.0	41.0
50-51	36.40949250468164	39.5	35.0	41.0	32.5	41.0
52-53	35.92835655213477	39.0	35.0	41.0	30.5	41.0
54-55	35.05450733378538	39.0	35.0	41.0	25.0	41.0
56-57	34.42396783239638	39.0	35.0	41.0	19.0	41.0
58-59	34.13109509972784	39.0	35.0	41.0	14.0	41.0
60-61	33.89733479965214	38.0	35.0	41.0	10.0	41.0
62-63	33.64531798519532	37.5	35.0	40.0	7.5	41.0
64-65	33.36040479039217	37.0	35.0	40.0	7.0	41.0
66-67	33.05558209162677	36.5	34.0	40.0	3.5	41.0
68-69	32.77821652557574	36.0	34.0	39.0	2.0	41.0
70-71	32.46960437566562	35.5	34.0	39.0	2.0	41.0
72-73	32.157551291418656	35.0	34.0	39.0	2.0	40.5
74-75	31.833461276223282	35.0	33.5	37.5	2.0	40.0
76-77	31.487945037462502	35.0	33.0	37.0	2.0	39.0
78-79	31.16876693983272	35.0	33.0	36.5	2.0	39.0
80-81	30.86061121452144	35.0	33.0	36.0	2.0	39.0
82-83	30.54454309643707	35.0	33.0	36.0	2.0	37.0
84-85	30.230218877195487	35.0	32.0	35.0	2.0	37.0
86-87	30.122369970642318	35.0	32.5	35.0	2.0	37.0
88-89	30.187298248118424	35.0	33.0	35.0	2.0	36.0
90-91	30.067993459792373	35.0	33.0	35.0	2.0	36.0
92-93	29.932665350611103	35.0	33.0	35.0	2.0	36.0
94-95	29.775988966921425	35.0	33.0	35.0	2.0	36.0
96-97	29.606953619371936	35.0	33.0	35.0	2.0	35.5
98-99	29.48441751393222	35.0	33.0	35.0	2.0	35.0
100	29.403020854659758	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.8201125967985377
1101	2	1.3577290863584999
1101	3	1.2399002667859342
1101	4	0.6166197805952862
1101	5	0.9315843084360722
1101	6	0.5380503355943063
1101	7	0.3210291639862177
1101	8	0.25569872390428117
1101	9	0.2572510437752129
1101	10-11	0.15246354850284405
1101	12-13	0.26226379487854246
1101	14-15	0.3630492139281998
1101	16-17	0.4244239812933088
1101	18-19	0.37635640747772925
1101	20-21	0.5016459204426482
1101	22-23	0.49816126489475465
1101	24-25	0.5038079619448226
1101	26-27	0.5356390350819282
1101	28-29	0.5378989552796085
1101	30-31	0.599487408390452
1101	32-33	0.5520989989150635
1101	34-35	0.5302894807804108
1101	36-37	0.5884355040117484
1101	38-39	0.6126849975637683
1101	40-41	0.5531386454198994
1101	42-43	0.6162775387408388
1101	44-45	0.6105344111211508
1101	46-47	0.5411520822424691
1101	48-49	0.4938484244946224
1101	50-51	0.4564008056885669
1101	52-53	0.44985924334717
1101	54-55	0.25716613216040685
1101	56-57	0.2926770259175271
1101	58-59	0.2312895258726897
1101	60-61	0.25562090680022465
1101	62-63	0.29917623842719365
1101	64-65	0.2605299289398104
1101	66-67	0.2388146845192054
1101	68-69	0.3066968448515439
1101	70-71	0.313794086496749
1101	72-73	0.328632466244116
1101	74-75	0.2552173690449706
1101	76-77	0.3232109803962011
1101	78-79	0.315032012964636
1101	80-81	0.321388593060302
1101	82-83	0.32806393974719583
1101	84-85	0.32690347416486176
1101	86-87	0.426828635485176
1101	88-89	0.3948405127182859
1101	90-91	0.37616127591665105
1101	92-93	0.3538143473980213
1101	94-95	0.33365630822074266
1101	96-97	0.34672571497584315
1101	98-99	0.35509371409733603
1101	100	0.3756602250220702
1102	1	1.3384887761860398
1102	2	1.1767152537781875
1102	3	1.0930408933804863
1102	4	0.5390983807616578
1102	5	0.7907386516436361
1102	6	0.44088047905359673
1102	7	0.18667462255186962
1102	8	0.1985726488873496
1102	9	0.2163715245292792
1102	10-11	0.1903605819045211
1102	12-13	0.2401039888377241
1102	14-15	0.3548118242131011
1102	16-17	0.3540250035064574
1102	18-19	0.4040242353636856
1102	20-21	0.4734428647814326
1102	22-23	0.5041888396009
1102	24-25	0.49796332566468493
1102	26-27	0.47933878258244533
1102	28-29	0.5437414461645744
1102	30-31	0.5167955868687812
1102	32-33	0.4925227859743515
1102	34-35	0.4712752840627559
1102	36-37	0.511986357221879
1102	38-39	0.5332753987178762
1102	40-41	0.515825256143934
1102	42-43	0.5261672692946675
1102	44-45	0.5419849572498521
1102	46-47	0.38692631090525964
1102	48-49	0.47862303816886964
1102	50-51	0.48832867325081253
1102	52-53	0.5111737675564996
1102	54-55	0.5187433996304947
1102	56-57	0.5201425681651912
1102	58-59	0.4743619482287116
1102	60-61	0.5241485394900209
1102	62-63	0.5227807458444573
1102	64-65	0.44870340907228723
1102	66-67	0.47671606669486977
1102	68-69	0.49308278559379204
1102	70-71	0.4965096426604916
1102	72-73	0.5156363194511542
1102	74-75	0.5545271509645815
1102	76-77	0.4868324229057954
1102	78-79	0.5215348796764552
1102	80-81	0.473400993057826
1102	82-83	0.47613151853538227
1102	84-85	0.48225312332510484
1102	86-87	0.4067820050312321
1102	88-89	0.38248761724657854
1102	90-91	0.372637416029896
1102	92-93	0.38420634395302145
1102	94-95	0.38436423064325
1102	96-97	0.4217647379744811
1102	98-99	0.4309883632111884
1102	100	0.4553426369564946
1103	1	1.8412306546412722
1103	2	1.3139347461465256
1103	3	1.21734365161603
1103	4	0.6063283069729906
1103	5	0.8406569074817938
1103	6	0.4006102235194504
1103	7	0.22020625122271298
1103	8	0.17142008016276833
1103	9	0.2121288001314383
1103	10-11	0.149250579624443
1103	12-13	0.22577914026475554
1103	14-15	0.34108756185193556
1103	16-17	0.33835017839518144
1103	18-19	0.4207797232511794
1103	20-21	0.5096930341351751
1103	22-23	0.55844513295132
1103	24-25	0.52122075822885
1103	26-27	0.49385456717769927
1103	28-29	0.5782114391591122
1103	30-31	0.5288799060336729
1103	32-33	0.48052624161687874
1103	34-35	0.4815712689745766
1103	36-37	0.5067565276660915
1103	38-39	0.5723268236024737
1103	40-41	0.49306380287399776
1103	42-43	0.5336116056097282
1103	44-45	0.5643118735907606
1103	46-47	0.5677643062856177
1103	48-49	0.5590604912867931
1103	50-51	0.5961032287178867
1103	52-53	0.4699917899343262
1103	54-55	0.4310033923276393
1103	56-57	0.44943696610849315
1103	58-59	0.41439254755923116
1103	60-61	0.42642440600292275
1103	62-63	0.409123212989833
1103	64-65	0.38457028133165494
1103	66-67	0.37468921214743744
1103	68-69	0.44965573492645916
1103	70-71	0.36801031924935756
1103	72-73	0.3482954045774349
1103	74-75	0.3093071604483839
1103	76-77	0.3460783939388641
1103	78-79	0.3840305838741145
1103	80-81	0.4330352178802954
1103	82-83	0.4119194967325335
1103	84-85	0.45807353896050174
1103	86-87	0.42913473749197095
1103	88-89	0.38403214406395136
1103	90-91	0.3715832922248765
1103	92-93	0.4185623329623738
1103	94-95	0.42958612122586004
1103	96-97	0.4282190777261832
1103	98-99	0.4431219395327268
1103	100	0.4762196167430943
1104	1	1.8277438126554912
1104	2	1.3466765551460078
1104	3	1.2195546578478904
1104	4	0.6153772635541443
1104	5	0.8757349809208463
1104	6	0.43723576340829595
1104	7	0.10089369485470456
1104	8	0.04590647065963793
1104	9	0.04625291708558876
1104	10-11	0.02414103998695083
1104	12-13	0.07404985236411932
1104	14-15	0.24003694206262338
1104	16-17	0.21993710381379117
1104	18-19	0.2569042666317145
1104	20-21	0.28700306463520064
1104	22-23	0.2670141146821834
1104	24-25	0.3161120776189392
1104	26-27	0.2604374953030657
1104	28-29	0.25874121581557574
1104	30-31	0.26107613263243934
1104	32-33	0.2584588128553307
1104	34-35	0.2695409418503303
1104	36-37	0.3071657099263163
1104	38-39	0.3066828326002806
1104	40-41	0.271825290324756
1104	42-43	0.2493368864100418
1104	44-45	0.30259546383932445
1104	46-47	0.3143015806365099
1104	48-49	0.2882992566407765
1104	50-51	0.3015329095918631
1104	52-53	0.4502445619781881
1104	54-55	0.4696386027240109
1104	56-57	0.438450112317895
1104	58-59	0.43719952097441706
1104	60-61	0.4439315061473721
1104	62-63	0.49195726302701104
1104	64-65	0.4781757835395197
1104	66-67	0.4355740632232923
1104	68-69	0.4516545177588327
1104	70-71	0.41347027675927706
1104	72-73	0.42641992216898217
1104	74-75	0.42878836227338013
1104	76-77	0.4777009426682497
1104	78-79	0.451578110982517
1104	80-81	0.3959346611650396
1104	82-83	0.46808940330738125
1104	84-85	0.4401477037332562
1104	86-87	0.4224855280334374
1104	88-89	0.4229581162270968
1104	90-91	0.433588801987824
1104	92-93	0.42104394196280737
1104	94-95	0.4279051331952246
1104	96-97	0.445410471613755
1104	98-99	0.460899106688597
1104	100	0.4447842561167761
1105	1	1.5746375122117087
1105	2	1.2607489868832964
1105	3	1.1737964155490737
1105	4	0.6040451787611545
1105	5	0.9515813472116648
1105	6	0.6468607093849172
1105	7	0.3406852963712268
1105	8	0.24164661732213233
1105	9	0.16985050527107148
1105	10-11	0.2191935524694273
1105	12-13	0.22313514430118886
1105	14-15	0.32498760949293626
1105	16-17	0.33314085716499164
1105	18-19	0.3679043052825861
1105	20-21	0.44697586847497917
1105	22-23	0.4852451168993426
1105	24-25	0.47618686705951774
1105	26-27	0.4549540782847288
1105	28-29	0.5331275453529258
1105	30-31	0.5380140347332869
1105	32-33	0.5665132157169026
1105	34-35	0.5198747448503411
1105	36-37	0.5430134289977175
1105	38-39	0.5344809801693202
1105	40-41	0.48367151958065335
1105	42-43	0.5377248463115123
1105	44-45	0.5712777150067581
1105	46-47	0.5802173955199095
1105	48-49	0.5594232767532858
1105	50-51	0.5135980433414531
1105	52-53	0.42377222721945884
1105	54-55	0.34981562372549035
1105	56-57	0.3068858763226885
1105	58-59	0.29303343114276004
1105	60-61	0.34172234518756994
1105	62-63	0.3479706362293058
1105	64-65	0.31375382492405635
1105	66-67	0.3666605217140031
1105	68-69	0.36470595153056706
1105	70-71	0.3101577211986495
1105	72-73	0.2820004934288889
1105	74-75	0.2967237731497363
1105	76-77	0.3000685546415873
1105	78-79	0.253203292378096
1105	80-81	0.2520196263265504
1105	82-83	0.25670830217912766
1105	84-85	0.2980739438113993
1105	86-87	0.22131554851840463
1105	88-89	0.1588791841155981
1105	90-91	0.18955816589985375
1105	92-93	0.24216119817241832
1105	94-95	0.21839859944071094
1105	96-97	0.27625597704420457
1105	98-99	0.21126119510071462
1105	100	0.2601796018488436
1106	1	1.618670882084544
1106	2	1.2731832187142764
1106	3	1.1347844408646814
1106	4	0.6587793357934189
1106	5	0.9757168305221029
1106	6	0.6603492821209116
1106	7	0.40403871550937964
1106	8	0.2894057059709638
1106	9	0.22666580084362664
1106	10-11	0.16620410780996764
1106	12-13	0.18880441634718892
1106	14-15	0.288753480272824
1106	16-17	0.30077132260927897
1106	18-19	0.29473782747525235
1106	20-21	0.3184470319823589
1106	22-23	0.3499868663960797
1106	24-25	0.34824451399715173
1106	26-27	0.31724512376439407
1106	28-29	0.3917982053175635
1106	30-31	0.40167656363566095
1106	32-33	0.35721942078368585
1106	34-35	0.3265149919364916
1106	36-37	0.3420639508158061
1106	38-39	0.3373797202537858
1106	40-41	0.3019891131424899
1106	42-43	0.29429713428673665
1106	44-45	0.30533182551626936
1106	46-47	0.3253334393989107
1106	48-49	0.30154964070389667
1106	50-51	0.3183124461860558
1106	52-53	0.31015567658798915
1106	54-55	0.0776716667003825
1106	56-57	-0.031389992294400315
1106	58-59	0.008634634845819278
1106	60-61	0.04203974818156553
1106	62-63	0.11561716718053106
1106	64-65	0.11765301252363969
1106	66-67	0.044483803490393825
1106	68-69	0.07922324606558817
1106	70-71	0.09706496323004643
1106	72-73	0.08628132084282925
1106	74-75	0.08495973847579563
1106	76-77	0.12532461546330964
1106	78-79	0.09447399163264691
1106	80-81	0.09802971734705679
1106	82-83	0.1918831666516212
1106	84-85	0.1612215026357724
1106	86-87	0.13177010153516377
1106	88-89	0.10667198344111739
1106	90-91	0.12253812249945639
1106	92-93	0.16327051806539572
1106	94-95	0.0832015776764905
1106	96-97	0.09803000728667755
1106	98-99	0.05116676319367741
1106	100	0.07855867622356882
1107	1	1.6031967125087192
1107	2	1.2509238299551377
1107	3	1.1939396694569346
1107	4	0.6522802120807469
1107	5	0.985502629493368
1107	6	0.6815402591163107
1107	7	0.4227653977808927
1107	8	0.33078419865119457
1107	9	0.289975907206113
1107	10-11	0.1375464676622542
1107	12-13	0.029817062525097526
1107	14-15	0.15267823447710782
1107	16-17	0.17387305125255637
1107	18-19	0.16743605882530943
1107	20-21	0.23892913259868465
1107	22-23	0.2486202007439431
1107	24-25	0.25339678026656287
1107	26-27	0.2394398147709751
1107	28-29	0.21216864265419133
1107	30-31	0.20070453955994338
1107	32-33	0.23739694231949926
1107	34-35	0.19252235099396842
1107	36-37	0.2094608913119913
1107	38-39	0.19088524463288792
1107	40-41	0.2105645929661577
1107	42-43	0.1887779819238986
1107	44-45	0.23821967860278903
1107	46-47	0.23524368222292225
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2216	4	-2.9119287646358103
2216	5	-3.9121549608883477
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2216	90-91	-1.2172813319424591
2216	92-93	-1.2246603201524167
2216	94-95	-1.2015207450494358
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2216	98-99	-1.2957932783676043
2216	100	-1.2704164328529224
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	9447.0
3	12634.0
4	15909.0
5	21272.0
6	14818.0
7	10200.0
8	8912.0
9	9389.0
10	9236.0
11	13117.0
12	9506.0
13	11846.0
14	10872.0
15	11269.0
16	13037.0
17	16548.0
18	22635.0
19	38195.0
20	87259.0
21	126875.0
22	57014.0
23	32575.0
24	30455.0
25	32202.0
26	32845.0
27	34835.0
28	40377.0
29	47806.0
30	56079.0
31	68514.0
32	88747.0
33	122682.0
34	184815.0
35	555397.0
36	554916.0
37	994063.0
38	1371416.0
39	542023.0
40	4253.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.583845193376728	45.17849263199675	25.986409617832052	16.251252556794476
2	16.456022644670632	42.28828378715963	24.614490260124455	16.641203308045284
3	17.832997432376846	26.997853113923952	19.976652848709335	35.19249660498986
4	32.65494795702075	28.03592810997067	22.185701821856455	17.12342211115212
5	16.170299815045865	41.32312272305983	23.536888074384397	18.969689387509906
6	35.54798854768483	24.771839310712323	23.946236553080784	15.733935588522066
7	18.120789107417558	25.085020820850527	39.54941688470489	17.244773187027025
8	34.72823953463474	25.365768906402902	23.160205034194277	16.74578652476808
9	34.30295699278173	27.48786154744844	22.795384664509136	15.413796795260698
10-11	15.772644201059732	45.004141630619145	23.0013486126007	16.221865555720427
12-13	15.716624185995842	26.89937434142438	31.617086057637223	25.766915414942552
14-15	16.148760679847577	35.6057654154898	22.6544953383155	25.590978566347122
16-17	25.592473143424492	26.47010678392491	31.3572630076904	16.580157064960197
18-19	16.66776233614263	26.79485686487014	31.38051536535568	25.15686543363154
20-21	25.81970477029446	35.94978578096503	22.364007069885556	15.866502378854957
22-23	25.2800555974258	27.05225171922361	31.403078989197475	16.264613694153113
24-25	16.35341894269032	35.91501926727081	22.286761605208312	25.44480018483056
26-27	16.895824774772493	30.999027540861785	35.95458689083005	16.150560793535675
28-29	25.684862260258413	26.74478306508409	31.463557362781724	16.106797311875773
30-31	22.7121836701592	28.59356813326382	31.90096052638623	16.793287670190754
32-33	18.779082620987367	29.718994237431207	33.037228196709854	18.464694944871567
34-35	18.945518925594627	29.495026581231727	33.2249286139893	18.334525879184348
36-37	19.07718621859927	29.40858684270375	33.64240461054624	17.87182232815075
38-39	19.849750272647597	29.10917270123553	33.17213386550888	17.86894316060799
40-41	18.36940639621058	30.555717822614408	33.22612437453875	17.84875140663626
42-43	18.35202960347127	29.27244209854049	33.900529706782876	18.474998591205367
44-45	18.331419694559774	29.994604243065464	33.08890413538513	18.58507192698963
46-47	19.06673492462276	29.271120920922673	33.58145870482876	18.080685449625808
48-49	18.40925862562709	29.474868233120187	33.59783293414507	18.51804020710766
50-51	19.16907274959747	30.131850620829038	32.763744211617706	17.935332417955788
52-53	19.239977377405694	29.647140873359117	32.80797410761059	18.304907641624595
54-55	19.09010441067573	30.90317995898112	30.95519556854808	19.05152006179507
56-57	19.474314021376486	31.470680802197215	30.077567970943264	18.97743720548304
58-59	20.87299063985344	31.44975431435083	28.81678749400638	18.860467551789352
60-61	20.178238527281323	32.153413580462	28.59988543459295	19.068462457663724
62-63	19.437735751242784	32.20330514743034	28.47490898654342	19.884050114783456
64-65	19.911596439436703	32.13403677971339	28.948352671220846	19.006014109629056
66-67	20.021723592873762	31.30429841623456	29.678156471855598	18.995821519036085
68-69	20.444713254243148	31.37820536901658	29.336282936504954	18.840798440235318
70-71	18.89684100587392	32.662293628475844	29.454544259166383	18.986321106483857
72-73	18.92434948658062	31.52412468128477	29.974192847930713	19.577332984203895
74-75	19.19421070580621	32.04016755359041	29.04653576593291	19.719085974670474
76-77	20.01780560186137	31.39322588798137	29.437061029409485	19.151907480747777
78-79	19.67594310265571	31.720218718023236	28.934141318353372	19.66969686096768
80-81	20.244147301372383	32.37052315762691	28.445781981698858	18.939547559301843
82-83	20.115137613041668	32.14406140171759	28.717434391641834	19.023366593598904
84-85	19.771013796106967	32.72947013034974	27.88653326356903	19.612982809974266
86-87	19.72137158973811	32.938623997607515	28.35214973193923	18.98785468071515
88-89	21.04940276661805	32.26084128739625	27.755773525299173	18.933982420686533
90-91	20.251314235410216	33.14426277982746	27.6364314716149	18.967991513147425
92-93	19.660167249705225	33.1173446542661	27.549264589920362	19.673223506108307
94-95	20.015074109676593	33.351575667099794	27.401866841607735	19.23148338161588
96-97	20.033053523000994	32.584413186319416	27.982767685917143	19.399765604762443
98-99	20.672869192763617	32.85963066634105	27.182778831883674	19.284721309011665
100	19.630841530572027	33.947802178190514	27.111754090729256	19.3096022005082
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	319829.0
1	169520.5
2	13908.0
3	6924.0
4	4944.0
5	4445.0
6	4612.5
7	5435.0
8	4989.0
9	4288.5
10	4352.5
11	6058.0
12	52183.0
13	58170.5
14	17898.5
15	16658.0
16	18670.0
17	20926.0
18	22957.5
19	24787.5
20	26762.0
21	29208.5
22	32518.0
23	37458.0
24	42252.5
25	47334.5
26	55065.5
27	63655.0
28	70267.0
29	74296.0
30	78421.5
31	84514.5
32	91612.5
33	99500.5
34	107348.5
35	114492.5
36	121662.0
37	127196.0
38	131223.5
39	132960.0
40	134252.0
41	137819.5
42	140788.0
43	143468.5
44	145375.5
45	146292.5
46	150753.0
47	158417.0
48	160631.0
49	173176.0
50	233201.5
51	230267.5
52	159221.5
53	133936.0
54	128317.5
55	124026.0
56	113777.0
57	99139.5
58	88915.5
59	74813.0
60	58910.0
61	48464.0
62	39058.0
63	29550.5
64	21300.0
65	15364.5
66	11297.0
67	8606.0
68	6712.5
69	5040.0
70	3735.0
71	2828.5
72	2140.0
73	1573.5
74	1100.0
75	771.0
76	577.0
77	376.5
78	234.0
79	156.0
80	80.0
81	49.0
82	34.5
83	13.5
84	9.5
85	8.5
86	7.0
87	7.0
88	4.5
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0539820698385985
2	0.0
3	0.0
4	4.695726325556585E-4
5	0.00954171589353098
6	1.8782905302226336E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	4.695726325556584E-5
16-17	2.7235212688228193E-4
18-19	0.0
20-21	0.0
22-23	4.695726325556584E-5
24-25	0.003662666533934136
26-27	0.007006023677730424
28-29	0.0010236683389713353
30-31	0.0055315656115056564
32-33	0.002798652890031724
34-35	0.025234833273541084
36-37	0.02485917516749656
38-39	0.022257742783138208
40-41	0.002235165730964934
42-43	0.005447042537645638
44-45	0.006808803172057047
46-47	0.0012866290132025043
48-49	0.008987620187115302
50-51	0.009457192819670961
52-53	0.0020473366779426706
54-55	0.011044348317709087
56-57	0.006320447634199162
58-59	0.011692358550635894
60-61	0.007391073236426064
62-63	9.767110757157695E-4
64-65	0.012678461079002779
66-67	0.005353128011134506
68-69	0.002066119583244897
70-71	0.008640136439024115
72-73	0.005108950242205564
74-75	0.008255086880328475
76-77	0.007222027088706027
78-79	0.01481971228345658
80-81	0.008292652690932928
82-83	0.011664184192682557
84-85	0.006583408308430332
86-87	0.012462457668027175
88-89	0.0193276095559909
90-91	0.017524450646977174
92-93	0.016369301970890253
94-95	0.005493999800901204
96-97	0.009447801367019847
98-99	0.01671678571898144
100	0.016078166938705746
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5323990.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.18003941362388
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.59805031890856	34.25178875398356
2	16.68634484990671	15.74524815375837
3	5.891327990405026	8.33859260357684
4	2.1921580559727403	4.137044139267481
5	0.9118723044449153	2.151108563195157
6	0.456052275553936	1.2909938601184536
7	0.264711903284668	0.8742382621158222
8	0.1634624571024419	0.6169732134992809
9	0.11781807144294709	0.5002795128883858
>10	0.5399326265269183	4.75548598798461
>50	0.13251007542987633	4.632615834662807
>100	0.03964941042255825	2.941882448639865
>500	0.002595607932086238	0.8296818560984387
>1k	0.0030747960833779147	3.645376738688708
>5k	2.7952691667070055E-4	0.9086783062881295
>10k+	1.5972966666897174E-4	14.380011765234169
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	393693	7.394698337149394	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	306649	5.759759128022404	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	51180	0.9613090933679439	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11743	0.22056765696404387	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7816	0.14680718784220106	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7494	0.14075909233488418	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA	7113	0.13360280541473596	No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6785	0.12744201247560571	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	6521	0.12248332547581794	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6464	0.12141269987359105	No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT	6038	0.11341118221484263	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.8174357953339506E-4	0.0	0.0	0.0	3.756581060445268E-5
2	2.8174357953339506E-4	0.0	0.0	0.0	3.756581060445268E-5
3	2.8174357953339506E-4	0.0	0.0	0.0	3.756581060445268E-5
4	3.0052648483562143E-4	0.0	0.0	0.0	5.6348715906679014E-5
5	3.0052648483562143E-4	0.0	0.0	0.0	1.1269743181335803E-4
6	3.0052648483562143E-4	0.0	0.0	0.0	1.8782905302226337E-4
7	3.1930939013784774E-4	0.0	0.0	0.0	2.0661195832448971E-4
8	3.1930939013784774E-4	0.0	0.0	0.0	2.2539486362671606E-4
9	3.1930939013784774E-4	0.0	0.0	0.0	2.2539486362671606E-4
10-11	3.2870084278896093E-4	0.0	0.0	9.39145265111317E-6	2.6296067423116874E-4
12-13	4.507897272534321E-4	0.0	0.0	1.878290530222634E-5	3.0991793748673456E-4
14-15	6.10444422322356E-4	0.0	0.0	1.878290530222634E-5	3.2870084278896093E-4
16-17	7.607076647401668E-4	0.0	0.0	3.756581060445268E-5	3.7565810604452674E-4
18-19	9.954939810179959E-4	0.0	0.0	5.6348715906679014E-5	3.8504955869563993E-4
20-21	0.001164540128738033	0.0	0.0	5.6348715906679014E-5	4.2261536930009256E-4
22-23	0.0012866290132025043	0.0	0.0	5.6348715906679014E-5	4.7896408520677156E-4
24-25	0.0014368922556203148	0.0	0.0	5.6348715906679014E-5	5.447042537645639E-4
26-27	0.0015589811400847859	0.0	0.0	6.574016855779219E-5	6.198358749734691E-4
28-29	0.0016341127612936913	0.0	0.0	8.452307386001853E-5	6.480102329268087E-4
30-31	0.001784376003711502	0.0	0.0	9.391452651113169E-5	6.761845908801482E-4
32-33	0.0019722050567337656	0.0	0.0	9.391452651113169E-5	7.23141854135714E-4
34-35	0.0022727315415693866	0.0	0.0	1.1269743181335803E-4	7.513162120890535E-4
36-37	0.0026859554582183663	0.0	0.0	1.1269743181335803E-4	7.982734753446193E-4
38-39	0.003108570827518459	0.0	0.0	1.1269743181335803E-4	8.170563806468457E-4
40-41	0.0035781434600741175	0.0	0.0	1.1269743181335803E-4	8.546221912512984E-4
42-43	0.004066498997932003	0.0	0.0	1.1269743181335803E-4	9.297538124602037E-4
44-45	0.004648769062301019	0.0	0.0	1.1269743181335803E-4	0.0010330597916224486
46-47	0.005343736558483393	0.0	0.0	1.1269743181335803E-4	0.0010894085075291275
48-49	0.006113835675874674	0.0	0.0	1.1269743181335803E-4	0.0011081914128313539
50-51	0.0069778493197770845	0.0	0.0	1.1269743181335803E-4	0.0011269743181335802
52-53	0.00802969201670176	0.0	0.0	1.1269743181335803E-4	0.0012021059393424857
54-55	0.009410235556415396	0.0	0.0	1.1269743181335803E-4	0.0012208888446447119
56-57	0.010922259433244614	0.0	0.0	1.1269743181335803E-4	0.0013054119185047304
58-59	0.012856898679373927	0.0	0.0	1.1269743181335803E-4	0.00133358627645807
60-61	0.015270502010710011	0.0	0.0	1.1269743181335803E-4	0.001389934992364749
62-63	0.01836968138557736	0.0	0.0	1.1269743181335803E-4	0.0014275008029692017
64-65	0.02205113082481372	0.0	0.0	1.1269743181335803E-4	0.001502632424178107
66-67	0.0268125973189281	0.0	0.0	1.1269743181335803E-4	0.0015777640453870125
68-69	0.03253199198345602	0.0	0.0	1.1269743181335803E-4	0.0016341127612936913
70-71	0.04005454555699767	0.0	0.0	1.1269743181335803E-4	0.0016341127612936913
72-73	0.050018876819828736	0.0	0.0	1.1269743181335803E-4	0.0016998529298514835
74-75	0.06228411398218254	0.0	0.0	1.1269743181335803E-4	0.0017655930984092757
76-77	0.07847497835270165	0.0	0.0	1.1269743181335803E-4	0.0018125503616648417
78-79	0.09917373999575507	0.0	0.0	1.1269743181335803E-4	0.0019158563408270862
80-81	0.1260051202199854	0.0	0.0	1.1269743181335803E-4	0.0020379452252915573
82-83	0.15997400445906174	0.0	0.0	1.1269743181335803E-4	0.0021788170150582554
84-85	0.20334373280190232	0.0	0.0	1.1269743181335803E-4	0.0023290802574760655
86-87	0.25626456849092505	0.0	0.0	1.1269743181335803E-4	0.002404211878684971
88	0.3020103343544973	0.0	0.0	1.1269743181335803E-4	0.0024417776892894237
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	2235	0.0	75.7329	1
AACGAGT	133770	0.0	64.405235	1
GTGGAAT	142715	0.0	63.36402	6
ACGAGTG	139200	0.0	63.16124	2
TGGAATC	144070	0.0	63.01862	7
GAATCAC	144860	0.0	62.691162	9
GGAATCA	145235	0.0	62.542236	8
AGTGGAA	142950	0.0	62.293316	5
CGAGTGG	142975	0.0	61.97346	3
GAGTGGA	146505	0.0	60.833065	4
ACGTGTG	2330	0.0	54.456177	2
CGTGTGG	2440	0.0	54.31235	3
AACGTGT	2125	0.0	53.323395	1
TCGAGTG	1430	0.0	46.993668	2
ATCGAGT	1305	0.0	46.837368	1
AGTACTT	57190	0.0	41.217617	20-21
GAGTACT	56550	0.0	40.574677	20-21
GTACTTT	56995	0.0	39.41403	22-23
TACTTTT	61335	0.0	37.866405	22-23
CATGGGG	26065	0.0	37.440716	24-25
>>END_MODULE