FastQCFastQC Report
Thu 26 May 2016
SRR2132697_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132697_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2694567
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2155908.000914432634259No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA474321.7602828209504533No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG134700.4998947882906605No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41650.1545702890297402No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT41360.1534940493222102No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC34510.12807252519607046No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA33120.122913996942737No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27840.10331901192288037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAGT582150.077.113391
ACGAGTG605250.075.7365952
TACGAGT12450.074.765741
CGAGTGG620950.074.5254753
GTGGAAT635700.074.2187966
GAATCAC641100.073.880869
TGGAATC641700.073.7884457
GGAATCA643950.073.486858
AGTGGAA636050.073.2364355
GAGTGGA640300.072.8310554
ATCGAGT5450.055.206511
TCGAGTG6850.050.0778432
CGTGTGG12300.044.6986123
AGTACTT299750.042.10207420-21
GAGTACT295350.041.9735120-21
AACGTGT9750.041.9489821
GTACTTT298650.040.81176822-23
ACGTGTG12450.038.8759652
CATGGGG149400.038.80273424-25
TACTTTT321750.038.61931622-23