##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132697_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2694567
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.853157483187466	33.0	31.0	34.0	25.0	34.0
2	31.59810500165704	34.0	31.0	34.0	28.0	34.0
3	31.799448668376034	34.0	31.0	34.0	28.0	34.0
4	35.75841535949932	37.0	35.0	37.0	35.0	37.0
5	35.41331056158559	37.0	35.0	37.0	33.0	37.0
6	35.77590017245814	37.0	35.0	37.0	35.0	37.0
7	35.95704912885818	37.0	35.0	37.0	35.0	37.0
8	36.10851576524169	37.0	37.0	37.0	35.0	37.0
9	38.03799051944153	39.0	39.0	39.0	37.0	39.0
10-11	38.117399010675925	39.0	39.0	39.0	37.0	39.0
12-13	38.10581087796295	39.0	39.0	39.0	37.0	39.0
14-15	39.62928663492131	41.0	40.0	41.0	37.5	41.0
16-17	39.57387309352486	41.0	40.0	41.0	37.0	41.0
18-19	39.512025865380224	41.0	40.0	41.0	37.0	41.0
20-21	39.39432866208189	41.0	39.5	41.0	36.0	41.0
22-23	39.30105356445024	41.0	39.0	41.0	36.0	41.0
24-25	39.265645092513935	41.0	39.0	41.0	36.0	41.0
26-27	39.180088118053845	41.0	39.0	41.0	35.5	41.0
28-29	39.01451494804175	41.0	39.0	41.0	35.0	41.0
30-31	38.91490840643414	41.0	39.0	41.0	35.0	41.0
32-33	38.766195459233344	41.0	39.0	41.0	35.0	41.0
34-35	38.69007339583688	41.0	39.0	41.0	35.0	41.0
36-37	38.56037185195247	40.5	39.0	41.0	35.0	41.0
38-39	38.10880393770131	40.0	37.0	41.0	35.0	41.0
40-41	37.884712645853675	40.0	36.5	41.0	34.0	41.0
42-43	37.77951930681256	40.0	36.0	41.0	34.0	41.0
44-45	37.62460183769785	40.0	35.5	41.0	33.5	41.0
46-47	37.52549296417569	40.0	35.0	41.0	33.0	41.0
48-49	37.37512594787957	40.0	35.0	41.0	33.0	41.0
50-51	37.119594539679284	40.0	35.0	41.0	33.0	41.0
52-53	36.62139909677511	40.0	35.0	41.0	32.0	41.0
54-55	35.699474535240725	39.5	35.0	41.0	28.5	41.0
56-57	34.990854931423115	39.0	35.0	41.0	22.5	41.0
58-59	34.64859845756294	39.0	35.0	41.0	20.0	41.0
60-61	34.4095969779189	39.0	35.0	41.0	19.0	41.0
62-63	34.13476784952833	38.0	35.0	41.0	18.0	41.0
64-65	33.849202858938	37.5	35.0	40.0	16.0	41.0
66-67	33.55593885770887	37.0	34.5	40.0	13.5	41.0
68-69	33.252049958304994	36.5	34.0	39.5	11.0	41.0
70-71	32.912095338508934	36.0	34.0	39.0	8.0	41.0
72-73	32.5750684618345	35.5	34.0	39.0	7.0	41.0
74-75	32.21844270341023	35.0	34.0	38.5	3.5	40.0
76-77	31.84104848756776	35.0	33.0	37.0	2.0	39.5
78-79	31.505765119219525	35.0	33.0	37.0	2.0	39.0
80-81	31.17182630827142	35.0	33.0	36.0	2.0	39.0
82-83	30.85076210760393	35.0	33.0	36.0	2.0	37.5
84-85	30.53087768832618	35.0	33.0	35.5	2.0	37.0
86-87	30.402873634242532	35.0	32.5	35.0	2.0	37.0
88-89	30.458733629559035	35.0	33.0	35.0	2.0	36.5
90-91	30.336578938285818	35.0	33.0	35.0	2.0	36.0
92-93	30.18738446659519	35.0	33.0	35.0	2.0	36.0
94-95	30.033283269631077	35.0	33.0	35.0	2.0	36.0
96-97	29.87877273046096	35.0	33.0	35.0	2.0	36.0
98-99	29.741924398242837	35.0	33.0	35.0	2.0	35.0
100	29.64919558504205	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.9230460906287163
1101	2	1.4244768468287674
1101	3	1.2749475653373779
1101	4	0.6318948555042994
1101	5	0.9643818920900245
1101	6	0.6563183760166993
1101	7	0.4454573595224929
1101	8	0.35524026093499117
1101	9	0.3527465360991613
1101	10-11	0.22438981833445126
1101	12-13	0.279249919875717
1101	14-15	0.3152700879372432
1101	16-17	0.33565983465038585
1101	18-19	0.3274146578165329
1101	20-21	0.3811790568236404
1101	22-23	0.3998092618794118
1101	24-25	0.38335703674803767
1101	26-27	0.44291487348375824
1101	28-29	0.5189958603756679
1101	30-31	0.556286671735748
1101	32-33	0.5111183314684382
1101	34-35	0.5204814337533605
1101	36-37	0.5271887651687024
1101	38-39	0.5952657937429251
1101	40-41	0.5340365769396413
1101	42-43	0.5612902734153096
1101	44-45	0.6040497132289673
1101	46-47	0.5945338625923711
1101	48-49	0.6222789676095175
1101	50-51	0.5295293112867512
1101	52-53	0.5846164709021977
1101	54-55	0.5307990563735245
1101	56-57	0.4662597045203327
1101	58-59	0.33798249086648013
1101	60-61	0.40032685046205074
1101	62-63	0.42602830905138944
1101	64-65	0.4195866822180321
1101	66-67	0.3729654972290106
1101	68-69	0.38940951956548986
1101	70-71	0.3232359500358868
1101	72-73	0.3287866180567889
1101	74-75	0.2919222195440412
1101	76-77	0.4089886630475519
1101	78-79	0.44300963930403725
1101	80-81	0.4090577035997214
1101	82-83	0.4289010560932951
1101	84-85	0.43949982126426335
1101	86-87	0.4491229682242839
1101	88-89	0.37720186316705195
1101	90-91	0.3607376000239775
1101	92-93	0.3896284301176678
1101	94-95	0.3425046306950428
1101	96-97	0.3497053132857708
1101	98-99	0.38638047228044314
1101	100	0.4595342189491447
1102	1	1.4306064972522208
1102	2	1.245596390300122
1102	3	1.1734039758587969
1102	4	0.6299535014214541
1102	5	0.9049618547349922
1102	6	0.5373895646595841
1102	7	0.30194045009736215
1102	8	0.26977313852714246
1102	9	0.2472517296292125
1102	10-11	0.21848170727338356
1102	12-13	0.16111737596879294
1102	14-15	0.2646149353796048
1102	16-17	0.21444742037869702
1102	18-19	0.2617050230246818
1102	20-21	0.3302330001414475
1102	22-23	0.3075431922041574
1102	24-25	0.32090330604382444
1102	26-27	0.2720084603784869
1102	28-29	0.3178634714994928
1102	30-31	0.3588225687362723
1102	32-33	0.2817521488422159
1102	34-35	0.2485989913546618
1102	36-37	0.26597088790875745
1102	38-39	0.2801374135189434
1102	40-41	0.2897948618566275
1102	42-43	0.3347063021181498
1102	44-45	0.31140215717796593
1102	46-47	0.23724154842625467
1102	48-49	0.34922788866933985
1102	50-51	0.3320424704442786
1102	52-53	0.24899468686740533
1102	54-55	0.3443099491291619
1102	56-57	0.2792240400659267
1102	58-59	0.2059277428652706
1102	60-61	0.22038502390454084
1102	62-63	0.2943819932463754
1102	64-65	0.24566602843122354
1102	66-67	0.20211906625829812
1102	68-69	0.14566759408359786
1102	70-71	0.19190789020878185
1102	72-73	0.23354875659521213
1102	74-75	0.2103586975407552
1102	76-77	0.23893691230746583
1102	78-79	0.28726936748346077
1102	80-81	0.28664070041789813
1102	82-83	0.13571797954501363
1102	84-85	0.1415628375720246
1102	86-87	0.13145215756112094
1102	88-89	0.19689744922018804
1102	90-91	0.16934981644689984
1102	92-93	0.1804634921668793
1102	94-95	0.17600791956161643
1102	96-97	0.1874214150745317
1102	98-99	0.2384610587396132
1102	100	0.273384887426964
1103	1	1.888748182872007
1103	2	1.3349618614256187
1103	3	1.2823165810575716
1103	4	0.6237444598872202
1103	5	0.9363684511376107
1103	6	0.5594931814167765
1103	7	0.434775310442177
1103	8	0.3291553984084743
1103	9	0.30804618216510704
1103	10-11	0.2153561646880533
1103	12-13	0.21729771877509307
1103	14-15	0.32321389935552247
1103	16-17	0.332164508318769
1103	18-19	0.3330178463337887
1103	20-21	0.4375590679134973
1103	22-23	0.45246455768963756
1103	24-25	0.33232443117745447
1103	26-27	0.35241576707461775
1103	28-29	0.3245942559074706
1103	30-31	0.4014370587240066
1103	32-33	0.35408718496673686
1103	34-35	0.3550379814464293
1103	36-37	0.3870912145002805
1103	38-39	0.4259284939543093
1103	40-41	0.4364543299257093
1103	42-43	0.4231457011667885
1103	44-45	0.45287267999703573
1103	46-47	0.517481146931047
1103	48-49	0.4883518599993124
1103	50-51	0.6080871041408855
1103	52-53	0.5532553000157279
1103	54-55	0.5974505585466474
1103	56-57	0.6612193908719988
1103	58-59	0.5926953333918163
1103	60-61	0.5694358852251185
1103	62-63	0.47979016090434357
1103	64-65	0.42941099718547804
1103	66-67	0.5505231658715815
1103	68-69	0.6579352240709042
1103	70-71	0.563171387579267
1103	72-73	0.5819753129631025
1103	74-75	0.5326414661070444
1103	76-77	0.5693876975764027
1103	78-79	0.5931846775373444
1103	80-81	0.6254276051883458
1103	82-83	0.49596521908612345
1103	84-85	0.48844113344155815
1103	86-87	0.5233336102907309
1103	88-89	0.5374743295249687
1103	90-91	0.5325379940059314
1103	92-93	0.5824909496748276
1103	94-95	0.5710445821865484
1103	96-97	0.51683997884669
1103	98-99	0.5447327352475497
1103	100	0.5068835493487747
1104	1	1.8941896942279044
1104	2	1.3528956624323811
1104	3	1.2396066503495717
1104	4	0.6618163350275026
1104	5	0.9922306579850115
1104	6	0.5933047596040097
1104	7	0.3805843027182476
1104	8	0.32958552920592155
1104	9	0.273778841292021
1104	10-11	0.23001008847295168
1104	12-13	0.22461579610082083
1104	14-15	0.3058018111906975
1104	16-17	0.3381450167224358
1104	18-19	0.32508283716450137
1104	20-21	0.3123854291884527
1104	22-23	0.33223229675756727
1104	24-25	0.30649434593549074
1104	26-27	0.29360215219138297
1104	28-29	0.3366383685259535
1104	30-31	0.35769225784082437
1104	32-33	0.3622940999791524
1104	34-35	0.30349277344573267
1104	36-37	0.38411524834435085
1104	38-39	0.4200695638320937
1104	40-41	0.3649126466560375
1104	42-43	0.34608713493426535
1104	44-45	0.3893723089581371
1104	46-47	0.403451699077948
1104	48-49	0.46540221017247063
1104	50-51	0.41860160264536717
1104	52-53	0.4805006395842142
1104	54-55	0.5848770088820316
1104	56-57	0.7168755381193037
1104	58-59	0.6486990005530231
1104	60-61	0.5831449968210904
1104	62-63	0.6406338975515098
1104	64-65	0.6327036349312536
1104	66-67	0.582988134700436
1104	68-69	0.5763339598230388
1104	70-71	0.536348447676481
1104	72-73	0.5123187165342742
1104	74-75	0.560836889720413
1104	76-77	0.6283877671288849
1104	78-79	0.6506412571184015
1104	80-81	0.5739395493712394
1104	82-83	0.5682978193012644
1104	84-85	0.6172356920548978
1104	86-87	0.49850923029147154
1104	88-89	0.4439735650019401
1104	90-91	0.48885540240864245
1104	92-93	0.5029276630183155
1104	94-95	0.47472808544775447
1104	96-97	0.46379166841764174
1104	98-99	0.5064683092051112
1104	100	0.5215846843010823
1105	1	1.6073171852129136
1105	2	1.2779682352856057
1105	3	1.2319956667232148
1105	4	0.5934226213678429
1105	5	0.9621619887533086
1105	6	0.6501218630737071
1105	7	0.34653113582440653
1105	8	0.27439565360420204
1105	9	0.2067357591132435
1105	10-11	0.19761002981078946
1105	12-13	0.1792116634380747
1105	14-15	0.21630859184598705
1105	16-17	0.17582041010395955
1105	18-19	0.2791389910836486
1105	20-21	0.3294163260293175
1105	22-23	0.3167598830685776
1105	24-25	0.33575937595671235
1105	26-27	0.367328568709965
1105	28-29	0.4057332782897518
1105	30-31	0.4060712843940806
1105	32-33	0.3833391490655771
1105	34-35	0.3741208251719499
1105	36-37	0.4405721834191425
1105	38-39	0.43164051269250336
1105	40-41	0.37385296438063875
1105	42-43	0.33701337158431244
1105	44-45	0.35774192152909023
1105	46-47	0.3449569549484721
1105	48-49	0.29519379776024834
1105	50-51	0.38000427522426605
1105	52-53	0.3856175649107456
1105	54-55	0.3286902540208274
1105	56-57	0.30092579347222426
1105	58-59	0.31436028697963536
1105	60-61	0.2569696508868944
1105	62-63	0.2819579369586762
1105	64-65	0.19821195762487775
1105	66-67	0.2294213892424395
1105	68-69	0.2980513052741358
1105	70-71	0.3648045006622098
1105	72-73	0.37023012804930744
1105	74-75	0.3867407614228213
1105	76-77	0.3180140007595469
1105	78-79	0.22242907264316614
1105	80-81	0.33771410336630225
1105	82-83	0.3238071017250448
1105	84-85	0.3083584037994065
1105	86-87	0.3026830320306324
1105	88-89	0.2921987112259963
1105	90-91	0.27372272241071727
1105	92-93	0.25691647407440854
1105	94-95	0.28200133032775554
1105	96-97	0.3619626815476167
1105	98-99	0.33413366316221627
1105	100	0.32830419723264015
1106	1	1.6275209688594288
1106	2	1.2603066973568033
1106	3	1.1498415968215596
1106	4	0.6336613789282097
1106	5	0.9738257313083665
1106	6	0.65702719111286
1106	7	0.4046161120467673
1106	8	0.3324950580088384
1106	9	0.28342443356728353
1106	10-11	0.23886597422189482
1106	12-13	0.22383298136595897
1106	14-15	0.3084498495217787
1106	16-17	0.30984884516559674
1106	18-19	0.3230143719876608
1106	20-21	0.3442989960510232
1106	22-23	0.39876750692009466
1106	24-25	0.37744600160886677
1106	26-27	0.3636674174564405
1106	28-29	0.4293298489993873
1106	30-31	0.4084115355598783
1106	32-33	0.4287590668826482
1106	34-35	0.3751726396601569
1106	36-37	0.4025048672919027
1106	38-39	0.413639191071006
1106	40-41	0.39216882037583645
1106	42-43	0.4297389706424326
1106	44-45	0.43349925681696533
1106	46-47	0.4450964754711251
1106	48-49	0.440548355911595
1106	50-51	0.3976989979723129
1106	52-53	0.41749879788548583
1106	54-55	0.38283128661697674
1106	56-57	0.44588718365644553
1106	58-59	0.43604469069171614
1106	60-61	0.32810563939052173
1106	62-63	0.3555330096767193
1106	64-65	0.4215349699984827
1106	66-67	0.3490108208874787
1106	68-69	0.36803592483182257
1106	70-71	0.380367459478407
1106	72-73	0.44154863305877967
1106	74-75	0.4249263886447565
1106	76-77	0.3879019514959623
1106	78-79	0.3951055581688152
1106	80-81	0.41423613231500767
1106	82-83	0.46643256919815457
1106	84-85	0.5151589832352101
1106	86-87	0.46633973940400963
1106	88-89	0.4644582222837528
1106	90-91	0.44037688465273916
1106	92-93	0.498484644350043
1106	94-95	0.43140515783595035
1106	96-97	0.40094330161317515
1106	98-99	0.4511822919077737
1106	100	0.4913123578125358
1107	1	1.657851613655751
1107	2	1.296455368089795
1107	3	1.214497098684589
1107	4	0.658136535941118
1107	5	1.0159894169506742
1107	6	0.65928948438129
1107	7	0.4089902226421813
1107	8	0.3025521056130387
1107	9	0.3330183873760717
1107	10-11	0.1983873802731111
1107	12-13	0.1432333781095494
1107	14-15	0.2574621390188838
1107	16-17	0.24740032377714272
1107	18-19	0.22586210089463066
1107	20-21	0.3175356493252792
1107	22-23	0.30591354275273375
1107	24-25	0.2611314302075911
1107	26-27	0.25024501376356056
1107	28-29	0.23777315841412872
1107	30-31	0.21627707770068128
1107	32-33	0.18324198025779026
1107	34-35	0.15254065579136977
1107	36-37	0.1939485437824544
1107	38-39	0.15474137391498033
1107	40-41	0.17946906576350585
1107	42-43	0.08878644661908908
1107	44-45	0.13745179592562096
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2214	4	-1.973081731516693
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2214	96-97	-0.6947326365940825
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2214	100	-0.7206231276490129
2215	1	-3.590548799666763
2215	2	-3.5518701458119466
2215	3	-3.5037648067566742
2215	4	-1.8823880249546576
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2215	12-13	-0.5176841550805804
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2216	1	-6.108445745652162
2216	2	-6.134363764407112
2216	3	-5.53332333510976
2216	4	-3.0054093711787786
2216	5	-3.8883581578846282
2216	6	-4.265219425653683
2216	7	-2.1068452616844624
2216	8	-1.319160620134241
2216	9	-1.0247031435318874
2216	10-11	-0.9051644875595883
2216	12-13	-0.793439827148724
2216	14-15	-1.0008512896002344
2216	16-17	-0.8926784603658575
2216	18-19	-0.9170771833061622
2216	20-21	-0.9392777557506307
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2216	26-27	-1.2998093496494363
2216	28-29	-1.8793085643495857
2216	30-31	-1.419588702457098
2216	32-33	-1.2310466616414288
2216	34-35	-1.0837552695209993
2216	36-37	-1.1336409418197988
2216	38-39	-1.1506429582925577
2216	40-41	-1.26196896422627
2216	42-43	-1.1348201698791343
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2216	50-51	-1.3333514253970904
2216	52-53	-1.4084665486872154
2216	54-55	-1.2223179823042187
2216	56-57	-1.095195281979855
2216	58-59	-1.1352733414875686
2216	60-61	-1.1535175946673348
2216	62-63	-1.1737570212545094
2216	64-65	-1.1535766773095375
2216	66-67	-1.1638951775575066
2216	68-69	-1.179709534017256
2216	70-71	-1.158615999439622
2216	72-73	-1.1529569514474574
2216	74-75	-1.2034387575018357
2216	76-77	-1.1516012912657594
2216	78-79	-1.1525463626435553
2216	80-81	-1.1963909304347453
2216	82-83	-1.2036727730753078
2216	84-85	-1.207755936454923
2216	86-87	-1.179548473420052
2216	88-89	-1.1413688131511321
2216	90-91	-1.1563457818751566
2216	92-93	-1.2350204937645763
2216	94-95	-1.2175544949930135
2216	96-97	-1.2546139666106555
2216	98-99	-1.3014279353128053
2216	100	-1.2891337790994832
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2949.0
3	1618.0
4	1977.0
5	2588.0
6	2079.0
7	1686.0
8	1799.0
9	2048.0
10	2430.0
11	6277.0
12	4021.0
13	5985.0
14	5325.0
15	5335.0
16	6284.0
17	8346.0
18	11267.0
19	20556.0
20	47419.0
21	69510.0
22	30825.0
23	17801.0
24	17173.0
25	18097.0
26	18348.0
27	18826.0
28	21306.0
29	24940.0
30	29220.0
31	35657.0
32	45861.0
33	61559.0
34	90152.0
35	182296.0
36	280195.0
37	525259.0
38	759690.0
39	305939.0
40	1924.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.695925580870316	44.183721855086624	25.14727610226392	16.973076461779137
2	17.170105623649366	41.42034694256999	24.848407926022993	16.561139507757648
3	18.15587371027468	24.63584117071172	20.672494197581354	36.535790921432245
4	34.720996498864004	25.37032102529769	22.21443697175859	17.69424550407971
5	17.34712132087951	40.867363489050696	23.296883896009614	18.488631294060184
6	37.085514898973784	23.075447162195665	23.25362175771224	16.58541618111832
7	18.28063655496412	23.13692700905192	40.93848844730898	17.64394798867499
8	36.057333144805824	23.344567049177105	23.151177907248176	17.446921898768895
9	35.79810782214731	24.538784895680827	23.02087867920894	16.642228602962923
10-11	16.675035358185564	43.20456681908448	23.087549873504724	17.03284794922524
12-13	16.606304463759855	24.29163943594648	32.24499520702213	26.857060893271534
14-15	16.935881925063832	33.43545885042456	22.96230182293213	26.666357401579475
16-17	26.575639919897746	23.915479952912467	32.263311655055595	17.245568472134185
18-19	17.21913019791306	24.108808576665563	32.25791008351249	26.41415114190889
20-21	26.737598285735704	33.63731538313948	22.772304418483564	16.85278191264125
22-23	26.366806379951353	24.260957052081856	32.319768749401575	17.052467818565216
24-25	17.07987012589371	33.48210798062911	22.835151666078733	26.60287022739845
26-27	17.410590930018373	28.251152448725055	37.32826004134916	17.009996579907412
28-29	26.12066327671434	23.99838339243761	32.91295722466039	16.967996106187663
30-31	23.67846473994618	25.440493196502555	33.33916018383157	17.541881879719703
32-33	19.6314654796486	26.72913505113968	34.63144877885665	19.00795069035507
34-35	19.42461412307414	26.465549066194637	34.94516214709808	19.164674663633143
36-37	19.415257125166395	26.62626554343848	35.125813782387034	18.832663549008092
38-39	20.01626233850646	26.413920502369287	34.656116129652524	18.91370102947173
40-41	19.169263622466644	27.366068893646727	34.52878659165463	18.935880892231992
42-43	19.312591114634458	26.64457275958207	34.94441079450476	19.09842533127871
44-45	19.249861052733156	27.034266854836993	34.52446631723401	19.19140577519585
46-47	19.732324425349795	26.619090041845876	34.68238715427419	18.966198378530137
48-49	19.257893283490134	26.844568343134213	34.63688000962025	19.2606583637554
50-51	19.599460488863304	27.266264111783023	33.9863251593833	19.147950239970367
52-53	19.8548494663043	27.10137230735202	33.82392291297166	19.21985531337203
54-55	20.13665716436848	27.893720744523403	32.15939958292823	19.81022250817989
56-57	20.455614175405522	28.610833269929874	30.98545679255192	19.94809576211269
58-59	21.22221684036158	29.03259570293688	29.927413546440167	19.817773910261373
60-61	20.945093939870244	29.344372209097052	29.61516042884451	20.095373422188192
62-63	20.29711292985681	29.51103550116885	29.57637154666248	20.61548002231186
64-65	20.571354868402985	29.39590826253336	30.04054992001366	19.99218694905
66-67	20.494275593929398	28.79191798778813	30.626927125163483	20.086879293118983
68-69	20.775240318820586	28.86258586261203	30.381238626109138	19.980935192458247
70-71	19.747930660518463	29.56238229947393	30.63604120938056	20.05364583062704
72-73	19.8233248726914	28.952189269966233	30.810703683181313	20.413782174161057
74-75	20.039746312452262	29.271086255696204	30.238466740253077	20.450700691598456
76-77	20.53498043956195	28.934231239803864	30.391284846408666	20.13950347422552
78-79	20.446507843655443	29.251968708521268	29.870085281008645	20.43143816681464
80-81	20.74092356569698	29.63781482663658	29.5444895595953	20.076772048071142
82-83	20.69213875660065	29.592894924474145	29.664510587946225	20.05045573097898
84-85	20.650254455948243	29.885880812477545	29.110228119609143	20.35363661196507
86-87	20.66565425300036	30.144737542922883	29.110208644686413	20.079399559390346
88-89	21.311059495163867	29.83069123816718	28.81682453042222	20.04142473624674
90-91	20.92286757525108	30.341533903397856	28.66784628969953	20.067752231651536
92-93	20.506260140179926	30.427759233201034	28.65145768044277	20.41452294617627
94-95	20.581283510481647	30.720481502464647	28.465359350014722	20.232875637038976
96-97	20.6078234733894	30.119136607551788	28.81980193757047	20.45323798148834
98-99	21.046926181970026	30.29716512782443	28.126441794645757	20.529466895559782
100	20.69367028279599	30.83069766785827	27.992419082502607	20.483212966843137
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	46263.0
1	25072.0
2	2857.5
3	1588.5
4	1279.0
5	1162.5
6	1155.5
7	1298.5
8	1252.5
9	1170.5
10	1291.5
11	2149.5
12	22630.0
13	25959.0
14	9028.0
15	8902.0
16	10033.5
17	11206.5
18	12229.0
19	13397.5
20	14682.0
21	16401.5
22	18526.0
23	21118.5
24	23786.5
25	26609.5
26	30617.0
27	34625.0
28	37782.0
29	40919.5
30	44473.5
31	48375.5
32	52747.0
33	57848.0
34	63561.0
35	67708.5
36	71422.0
37	74639.5
38	76278.5
39	77978.0
40	79741.5
41	80544.0
42	79699.5
43	80235.5
44	81710.0
45	82242.5
46	83185.5
47	84698.0
48	83936.5
49	84672.5
50	96852.5
51	95317.5
52	77646.0
53	70270.5
54	67655.0
55	65268.0
56	59453.5
57	51410.5
58	45910.0
59	38901.5
60	30611.0
61	24551.0
62	19433.0
63	14469.0
64	10642.5
65	7827.0
66	5724.0
67	4189.0
68	3043.5
69	2199.0
70	1597.5
71	1140.0
72	823.5
73	612.0
74	462.0
75	354.0
76	235.0
77	153.5
78	100.0
79	62.0
80	36.0
81	20.0
82	9.5
83	8.0
84	6.0
85	2.5
86	1.5
87	3.0
88	3.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.055519124222927096
2	0.0
3	3.711171405275875E-5
4	4.824522826858638E-4
5	0.008943923086714859
6	7.051225670024163E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	2.5978199836931127E-4
16-17	4.0822885458034633E-4
18-19	0.0
20-21	0.0
22-23	3.711171405275875E-5
24-25	0.004137956116882602
26-27	0.007255340097314337
28-29	0.0013174658488729358
30-31	0.005344086823597261
32-33	0.003117383980431736
34-35	0.024883404272374746
36-37	0.02467928984508457
38-39	0.022730924857314736
40-41	0.0024493731274820778
42-43	0.005900762534388642
44-45	0.007403786953525372
46-47	0.0013545775629256946
48-49	0.009315040227242447
50-51	0.010242833078561416
52-53	0.0020040325588489725
54-55	0.011374740357170558
56-57	0.006754331957602094
58-59	0.012302533208489527
60-61	0.007812015808105717
62-63	0.0013360217058993153
64-65	0.013248881916834876
66-67	0.005158528253333467
68-69	0.0027277109828777683
70-71	0.008498582518081754
72-73	0.004824522826858638
74-75	0.008498582518081754
76-77	0.0083686915188971
78-79	0.015401361331894884
80-81	0.008424359089976238
82-83	0.012024195353093837
84-85	0.007088337384076922
86-87	0.012822097205228151
88-89	0.019465094020671966
90-91	0.018036293029640757
92-93	0.016997165036163508
94-95	0.006216212103837091
96-97	0.00983460422398107
98-99	0.016403377611319367
100	0.0161064838988973
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2694567.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.535547632557396
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.51525771517335	54.332587298728185
2	10.356485967957125	13.160100150461046
3	1.9602933650583754	3.7364493740835787
4	0.6442629958732171	1.6373440904878764
5	0.3278385544067478	1.0414701044649344
6	0.2063435499420486	0.786609026760841
7	0.16041055342278812	0.7134240650430914
8	0.11084172213825062	0.5633911613271613
9	0.0762956353714731	0.43627364777704153
>10	0.6001506000057154	8.202020873615744
>50	0.024957529565736165	1.0767227839224793
>100	0.013296225424395085	1.7921793742133756
>500	0.0027472545256138183	1.2313455581854942
>1k	6.4297446343828E-4	1.0217390214945943
>5k	0.0	0.0
>10k+	1.7535667184680363E-4	10.26834346943455
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	215590	8.000914432634259	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	47432	1.7602828209504533	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	13470	0.4998947882906605	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4165	0.1545702890297402	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4136	0.1534940493222102	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	3451	0.12807252519607046	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA	3312	0.122913996942737	No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2784	0.10331901192288037	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9689371242207E-4	0.0	0.0	3.711171405275875E-5	3.711171405275875E-5
2	3.3400542647482877E-4	0.0	0.0	3.711171405275875E-5	3.711171405275875E-5
3	3.3400542647482877E-4	0.0	0.0	7.42234281055175E-5	3.711171405275875E-5
4	3.3400542647482877E-4	0.0	0.0	7.42234281055175E-5	3.711171405275875E-5
5	3.3400542647482877E-4	0.0	0.0	7.42234281055175E-5	7.42234281055175E-5
6	3.3400542647482877E-4	0.0	0.0	1.1133514215827627E-4	7.42234281055175E-5
7	3.3400542647482877E-4	0.0	0.0	1.1133514215827627E-4	7.42234281055175E-5
8	3.711171405275875E-4	0.0	0.0	1.1133514215827627E-4	7.42234281055175E-5
9	3.711171405275875E-4	0.0	0.0	1.1133514215827627E-4	7.42234281055175E-5
10-11	3.711171405275875E-4	0.0	0.0	1.48446856211035E-4	7.42234281055175E-5
12-13	3.711171405275875E-4	0.0	0.0	1.48446856211035E-4	1.1133514215827627E-4
14-15	4.6389642565948443E-4	0.0	0.0	2.4122614134293192E-4	1.2989099918465563E-4
16-17	4.8245228268586383E-4	0.0	0.0	3.3400542647482877E-4	1.48446856211035E-4
18-19	5.381198537650019E-4	0.0	0.0	3.711171405275875E-4	1.48446856211035E-4
20-21	6.123432818705194E-4	0.0	0.0	3.711171405275875E-4	1.48446856211035E-4
22-23	7.236784240287958E-4	0.0	0.0	3.896729975539669E-4	1.48446856211035E-4
24-25	7.979018521343133E-4	0.0	0.0	4.0822885458034633E-4	1.48446856211035E-4
26-27	9.277928513189689E-4	0.0	0.0	4.0822885458034633E-4	1.48446856211035E-4
28-29	9.463487083453483E-4	0.0	0.0	4.2678471160672573E-4	1.48446856211035E-4
30-31	0.0010020162794244865	0.0	0.0	4.453405686331051E-4	1.48446856211035E-4
32-33	0.0010576838505036246	0.0	0.0	4.8245228268586383E-4	1.48446856211035E-4
34-35	0.0012061307067146596	0.0	0.0	5.195639967386225E-4	1.6700271323741439E-4
36-37	0.0013731334199520739	0.0	0.0	5.195639967386225E-4	2.2267028431655254E-4
38-39	0.0016143595612950058	0.0	0.0	5.195639967386225E-4	2.2267028431655254E-4
40-41	0.0019483649877698348	0.0	0.0	5.195639967386225E-4	2.2267028431655254E-4
42-43	0.0022267028431655254	0.0	0.0	5.381198537650019E-4	2.5978199836931127E-4
44-45	0.0026720434117986306	0.0	0.0	5.566757107913813E-4	3.154495694484494E-4
46-47	0.003210163265563632	0.0	0.0	5.9378742484414E-4	3.896729975539669E-4
48-49	0.0036740596912231164	0.0	0.0	5.9378742484414E-4	4.2678471160672573E-4
50-51	0.0043420705441727745	0.0	0.0	5.9378742484414E-4	4.453405686331051E-4
52-53	0.005306975109544502	0.0	0.0	5.9378742484414E-4	4.8245228268586383E-4
54-55	0.006475994102206403	3.711171405275875E-5	0.0	6.123432818705194E-4	4.8245228268586383E-4
56-57	0.007663568951894683	3.711171405275875E-5	0.0	6.680108529496576E-4	4.8245228268586383E-4
58-59	0.009352151941295206	3.711171405275875E-5	0.0	7.051225670024163E-4	5.9378742484414E-4
60-61	0.011745857497698145	3.711171405275875E-5	0.0	7.42234281055175E-4	6.680108529496575E-4
62-63	0.015345693760815744	3.711171405275875E-5	0.0	7.607901380815544E-4	6.680108529496575E-4
64-65	0.019502205734724727	3.711171405275875E-5	0.0	8.164577091606927E-4	6.680108529496575E-4
66-67	0.025792641266667332	3.711171405275875E-5	0.0	8.535694232134514E-4	6.680108529496575E-4
68-69	0.03250986151021667	3.711171405275875E-5	0.0	8.535694232134514E-4	6.680108529496575E-4
70-71	0.04189912516556464	3.711171405275875E-5	0.0	8.535694232134514E-4	6.865667099760369E-4
72-73	0.05427588180215968	3.711171405275875E-5	0.0	8.535694232134514E-4	7.051225670024163E-4
74-75	0.06934323770757973	7.42234281055175E-5	0.0	9.092369942925896E-4	7.42234281055175E-4
76-77	0.09010724172009826	7.42234281055175E-5	0.0	9.277928513189689E-4	7.42234281055175E-4
78-79	0.11641944698350422	7.42234281055175E-5	0.0	0.0010020162794244865	7.42234281055175E-4
80-81	0.1519724690460471	7.42234281055175E-5	0.0	0.0010020162794244865	7.42234281055175E-4
82-83	0.1950591690613	7.42234281055175E-5	0.0	0.0010205721364508658	7.42234281055175E-4
84-85	0.2495020535766971	7.42234281055175E-5	0.0	0.001039127993477245	7.42234281055175E-4
86-87	0.32057098598773015	7.42234281055175E-5	0.0	0.001039127993477245	7.42234281055175E-4
88	0.3828444421682593	7.42234281055175E-5	0.0	0.001039127993477245	7.42234281055175E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGAGT	58215	0.0	77.11339	1
ACGAGTG	60525	0.0	75.736595	2
TACGAGT	1245	0.0	74.76574	1
CGAGTGG	62095	0.0	74.525475	3
GTGGAAT	63570	0.0	74.218796	6
GAATCAC	64110	0.0	73.88086	9
TGGAATC	64170	0.0	73.788445	7
GGAATCA	64395	0.0	73.48685	8
AGTGGAA	63605	0.0	73.236435	5
GAGTGGA	64030	0.0	72.831055	4
ATCGAGT	545	0.0	55.20651	1
TCGAGTG	685	0.0	50.077843	2
CGTGTGG	1230	0.0	44.698612	3
AGTACTT	29975	0.0	42.102074	20-21
GAGTACT	29535	0.0	41.97351	20-21
AACGTGT	975	0.0	41.948982	1
GTACTTT	29865	0.0	40.811768	22-23
ACGTGTG	1245	0.0	38.875965	2
CATGGGG	14940	0.0	38.802734	24-25
TACTTTT	32175	0.0	38.619316	22-23
>>END_MODULE