FastQCFastQC Report
Thu 26 May 2016
SRR2132696_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132696_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3286586
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2217336.746605748335812No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA456541.3891010306743838No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG154130.46896688539414455No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT43030.13092613429254552No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT41630.1266663948547216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAGT622800.076.496391
ACGAGTG646450.075.295822
CGAGTGG664950.073.794623
GTGGAAT681250.073.4305656
TGGAATC686150.073.159557
GAATCAC688550.072.972799
GGAATCA690000.072.85358
AGTGGAA680850.072.513015
GAGTGGA686400.071.960924
TACGAGT13500.070.7060551
AACGTGT11750.046.821181
ATCGAGT7500.044.5134621
TCGAGTG8600.043.1676672
AGTACTT320150.041.38773320-21
GAGTACT315800.041.33281720-21
ACGTGTG15100.040.457252
GTACTTT316800.040.39541222-23
CATGGGG174150.038.9957524-25
CGTGTGG16200.038.8704643
TACTTTT337550.038.6640522-23
Produced by FastQC (version 0.11.5)