##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2132696_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3286586 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.884405885012594 33.0 31.0 34.0 25.0 34.0 2 31.63181155156141 34.0 31.0 34.0 28.0 34.0 3 31.828227224238162 34.0 31.0 34.0 28.0 34.0 4 35.79311327925087 37.0 35.0 37.0 35.0 37.0 5 35.44754009175479 37.0 35.0 37.0 33.0 37.0 6 35.814273230641156 37.0 36.0 37.0 35.0 37.0 7 36.01151742263857 37.0 35.0 37.0 35.0 37.0 8 36.15246794089673 37.0 37.0 37.0 35.0 37.0 9 38.08401940493874 39.0 39.0 39.0 37.0 39.0 10-11 38.15731491584276 39.0 39.0 39.0 37.0 39.0 12-13 38.143443987164794 39.0 39.0 39.0 37.0 39.0 14-15 39.67899546824577 41.0 40.0 41.0 38.0 41.0 16-17 39.623070718368545 41.0 40.0 41.0 37.5 41.0 18-19 39.572982115788236 41.0 40.0 41.0 37.0 41.0 20-21 39.46131122082306 41.0 40.0 41.0 37.0 41.0 22-23 39.378708787781605 41.0 39.5 41.0 37.0 41.0 24-25 39.331110763570464 41.0 39.0 41.0 36.0 41.0 26-27 39.260097255936714 41.0 39.0 41.0 36.0 41.0 28-29 39.115592897919 41.0 39.0 41.0 35.5 41.0 30-31 39.02877819110773 41.0 39.0 41.0 35.0 41.0 32-33 38.89342420980312 41.0 39.0 41.0 35.0 41.0 34-35 38.803884791087164 41.0 39.0 41.0 35.0 41.0 36-37 38.68297193501098 40.5 39.0 41.0 35.0 41.0 38-39 38.283589262535656 40.0 37.5 41.0 35.0 41.0 40-41 38.07450238636689 40.0 37.0 41.0 35.0 41.0 42-43 37.96613035532921 40.0 37.0 41.0 34.5 41.0 44-45 37.8091761481367 40.0 36.5 41.0 34.0 41.0 46-47 37.70738556666401 40.0 36.0 41.0 34.0 41.0 48-49 37.551612220097084 40.0 36.0 41.0 33.0 41.0 50-51 37.311395320250256 40.0 35.0 41.0 33.0 41.0 52-53 36.86966809935903 40.0 35.0 41.0 32.5 41.0 54-55 36.05625472755011 39.5 35.0 41.0 30.0 41.0 56-57 35.41997288371581 39.0 35.0 41.0 26.5 41.0 58-59 35.093801744424155 39.0 35.0 41.0 25.5 41.0 60-61 34.84614642671757 39.0 35.0 41.0 25.0 41.0 62-63 34.563518496092904 38.0 35.0 40.5 25.0 41.0 64-65 34.26618640132953 37.5 35.0 40.0 23.5 41.0 66-67 33.961137180040325 37.0 35.0 40.0 23.0 41.0 68-69 33.64702734083332 36.0 34.0 39.5 22.5 41.0 70-71 33.29583859968977 36.0 34.0 39.0 22.0 41.0 72-73 32.943326448783026 35.0 34.0 39.0 20.5 40.5 74-75 32.579271468934635 35.0 34.0 37.5 19.5 40.0 76-77 32.19514566179008 35.0 34.0 37.0 18.0 39.0 78-79 31.862334957916815 35.0 33.5 36.5 15.5 39.0 80-81 31.54329234044081 35.0 33.0 36.0 11.0 38.5 82-83 31.235145223645446 35.0 33.0 36.0 7.0 37.0 84-85 30.93255493694673 35.0 33.0 35.0 4.0 37.0 86-87 30.815898929770896 35.0 33.0 35.0 2.0 36.5 88-89 30.911193560734453 35.0 33.5 35.0 2.0 36.0 90-91 30.805967955805812 35.0 34.0 35.0 2.0 36.0 92-93 30.675847368667668 35.0 34.0 35.0 2.0 36.0 94-95 30.534395570357812 35.0 33.0 35.0 2.0 36.0 96-97 30.38703596984835 35.0 33.0 35.0 2.0 35.5 98-99 30.260459942323127 35.0 33.0 35.0 2.0 35.0 100 30.17098776663687 35.0 33.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.9060215555647382 1101 2 1.4195780035895353 1101 3 1.2747278495791647 1101 4 0.6393931688172145 1101 5 0.9576043713193627 1101 6 0.5683454484533215 1101 7 0.3752001661284865 1101 8 0.28643043965190884 1101 9 0.2968868482235436 1101 10-11 0.19800580818494495 1101 12-13 0.22942489938144917 1101 14-15 0.32853987212505587 1101 16-17 0.31819329168732224 1101 18-19 0.31873367064130065 1101 20-21 0.37290931484147904 1101 22-23 0.38711862937070407 1101 24-25 0.3567732193996278 1101 26-27 0.3579683392063018 1101 28-29 0.44018537311787753 1101 30-31 0.4621069881626312 1101 32-33 0.3919645937717462 1101 34-35 0.38857751017967956 1101 36-37 0.3796361847201979 1101 38-39 0.3840011289548002 1101 40-41 0.3773092905878741 1101 42-43 0.3603232752489589 1101 44-45 0.4301932503489496 1101 46-47 0.43607016472429905 1101 48-49 0.38969114340473965 1101 50-51 0.38906064304202204 1101 52-53 0.4417069583095028 1101 54-55 0.26700347932016655 1101 56-57 0.13789327326377077 1101 58-59 0.1092617367971016 1101 60-61 0.10184222869468584 1101 62-63 0.13391014151864766 1101 64-65 0.17367774367708932 1101 66-67 0.20411945250991437 1101 68-69 0.2659338807454006 1101 70-71 0.18562701189622288 1101 72-73 0.23599677749692916 1101 74-75 0.1737764566358635 1101 76-77 0.21615343312938506 1101 78-79 0.2306239413636355 1101 80-81 0.2020672213085284 1101 82-83 0.15689651285837058 1101 84-85 0.17427008462481197 1101 86-87 0.12688436754223886 1101 88-89 0.11010110188868083 1101 90-91 0.1438245049619411 1101 92-93 0.1535145457359839 1101 94-95 0.11068399610376645 1101 96-97 0.13138478417832644 1101 98-99 0.1265887730857962 1101 100 0.1521184793565098 1102 1 1.3278135026978184 1102 2 1.1089603008340774 1102 3 1.0921861355234768 1102 4 0.5197128106703204 1102 5 0.8217472075219803 1102 6 0.48418458489025085 1102 7 0.24939885026869746 1102 8 0.17870961620658932 1102 9 0.16999867130287782 1102 10-11 0.11289560156726708 1102 12-13 0.10349887969324101 1102 14-15 0.2102162541263226 1102 16-17 0.20851783730751094 1102 18-19 0.21960436277255013 1102 20-21 0.29073074208460525 1102 22-23 0.25149805905228106 1102 24-25 0.23861649825395403 1102 26-27 0.17718146429987058 1102 28-29 0.16880101060291253 1102 30-31 0.25839640855861035 1102 32-33 0.24079175270286157 1102 34-35 0.23511772903958672 1102 36-37 0.22728420488283518 1102 38-39 0.31047196998054005 1102 40-41 0.2288795430678121 1102 42-43 0.2519404901280282 1102 44-45 0.2816751318828139 1102 46-47 0.2099378211455587 1102 48-49 0.2920729709002785 1102 50-51 0.22382088196081185 1102 52-53 0.2327810083179287 1102 54-55 0.27115449159333593 1102 56-57 0.3303310487099935 1102 58-59 0.2795852885371559 1102 60-61 0.22566101934258143 1102 62-63 0.18020049953739914 1102 64-65 0.1596538761769395 1102 66-67 0.22727732849429572 1102 68-69 0.2530497718573841 1102 70-71 0.22581774312009628 1102 72-73 0.2783479478532982 1102 74-75 0.2566457448332855 1102 76-77 0.23676370991250195 1102 78-79 0.2529999180930851 1102 80-81 0.204989635212101 1102 82-83 0.10178515609009153 1102 84-85 0.1287817066153174 1102 86-87 0.1302857504108097 1102 88-89 0.12109738546455873 1102 90-91 0.19458974578566313 1102 92-93 0.23624264357129476 1102 94-95 0.20518543238557996 1102 96-97 0.19249466075278931 1102 98-99 0.23484779200108363 1102 100 0.23997888982900406 1103 1 1.8732248235547608 1103 2 1.368375919494504 1103 3 1.2289096471019647 1103 4 0.6069368524350196 1103 5 0.8995412858434264 1103 6 0.540338359744581 1103 7 0.35607137356393537 1103 8 0.279764616006446 1103 9 0.30443188800590093 1103 10-11 0.17356140039360213 1103 12-13 0.15675143173285022 1103 14-15 0.28433715484779754 1103 16-17 0.2821096933062819 1103 18-19 0.28368300231790755 1103 20-21 0.34576219939430075 1103 22-23 0.3711841541101677 1103 24-25 0.33181200295668134 1103 26-27 0.3082257355719733 1103 28-29 0.3059999927549555 1103 30-31 0.3103214784142807 1103 32-33 0.30699882318163674 1103 34-35 0.23601174765325084 1103 36-37 0.32059629423964964 1103 38-39 0.3840652718725508 1103 40-41 0.37290633744793666 1103 42-43 0.4166586254188829 1103 44-45 0.3764192643339399 1103 46-47 0.44398379720187364 1103 48-49 0.4283952090577614 1103 50-51 0.47929644157095197 1103 52-53 0.48461215599338914 1103 54-55 0.42861705855912646 1103 56-57 0.32686082885963685 1103 58-59 0.35844689265980634 1103 60-61 0.34531522864978115 1103 62-63 0.2541536005446048 1103 64-65 0.22979088445338647 1103 66-67 0.3122671608209089 1103 68-69 0.35153237540274773 1103 70-71 0.35723634853933106 1103 72-73 0.4118280926191744 1103 74-75 0.33177329164102787 1103 76-77 0.31913190951517123 1103 78-79 0.3080061864207302 1103 80-81 0.3229637926863127 1103 82-83 0.2515762701782229 1103 84-85 0.21885743032673233 1103 86-87 0.2417460066870163 1103 88-89 0.2791612157148684 1103 90-91 0.22407554201546986 1103 92-93 0.17206948826851232 1103 94-95 0.21493273134287705 1103 96-97 0.22475992036748593 1103 98-99 0.1970543498726549 1103 100 0.20111221442610017 1104 1 1.8753460097690855 1104 2 1.3722452633883648 1104 3 1.2638899958447638 1104 4 0.6108697604298499 1104 5 0.929080962931998 1104 6 0.5723778544969207 1104 7 0.3236527160512708 1104 8 0.2617498928991111 1104 9 0.20295653430983407 1104 10-11 0.21149832509863842 1104 12-13 0.2001382052799343 1104 14-15 0.292624083006352 1104 16-17 0.3004019055929916 1104 18-19 0.2740187956205915 1104 20-21 0.2829903795434632 1104 22-23 0.3067089515962067 1104 24-25 0.2838635391454716 1104 26-27 0.23951905250228123 1104 28-29 0.3000767942248572 1104 30-31 0.3053067973288037 1104 32-33 0.27390337221704897 1104 34-35 0.1865709281980017 1104 36-37 0.2394078387555254 1104 38-39 0.2530279260311943 1104 40-41 0.2180956072867204 1104 42-43 0.21494623462138662 1104 44-45 0.17053799106192002 1104 46-47 0.2146488197028873 1104 48-49 0.22509543042119873 1104 50-51 0.19482339811117555 1104 52-53 0.21301086712981032 1104 54-55 0.114380704433664 1104 56-57 0.1885703167677235 1104 58-59 0.18252010536625818 1104 60-61 0.1478147323571264 1104 62-63 0.18598878211526682 1104 64-65 0.1979875332341976 1104 66-67 0.13134317739974222 1104 68-69 0.1528042853349305 1104 70-71 0.17746678289562823 1104 72-73 0.18228761856428832 1104 74-75 0.17172786939137552 1104 76-77 0.24111665992705156 1104 78-79 0.2165910592273299 1104 80-81 0.16038317229875432 1104 82-83 0.25810311031305844 1104 84-85 0.2479570854093751 1104 86-87 0.26652523494347946 1104 88-89 0.19391880995855004 1104 90-91 0.13262189478367503 1104 92-93 0.09731924060519503 1104 94-95 0.10634510537993691 1104 96-97 0.18107899379996084 1104 98-99 0.18408877060614515 1104 100 0.11683524882944596 1105 1 1.578297667655665 1105 2 1.2691477011530594 1105 3 1.1682519087227092 1105 4 0.5809118716344202 1105 5 0.9643866795787446 1105 6 0.6303342528914229 1105 7 0.3405225605183446 1105 8 0.27169675125199433 1105 9 0.2390491383796629 1105 10-11 0.25071173280445436 1105 12-13 0.20228531412347905 1105 14-15 0.2467813885532948 1105 16-17 0.2295362925364941 1105 18-19 0.22674223586437137 1105 20-21 0.29393426631726527 1105 22-23 0.27014349569583374 1105 24-25 0.34721185027035517 1105 26-27 0.2950603742370532 1105 28-29 0.3405188604222644 1105 30-31 0.33925704228579434 1105 32-33 0.3465889160658193 1105 34-35 0.34249554858845954 1105 36-37 0.373619503356295 1105 38-39 0.333867512408041 1105 40-41 0.3579471390057023 1105 42-43 0.41310283185898555 1105 44-45 0.4490894062049833 1105 46-47 0.3958093587558196 1105 48-49 0.3943009434290019 1105 50-51 0.29866505407941446 1105 52-53 0.23837535131828247 1105 54-55 0.21317980650399448 1105 56-57 0.2483113688284675 1105 58-59 0.19176630927081106 1105 60-61 0.1719304062839626 1105 62-63 0.2444978093704222 1105 64-65 0.11955006551391989 1105 66-67 0.24303913560949297 1105 68-69 0.30843732759738174 1105 70-71 0.2638023149392552 1105 72-73 0.2905980332941738 1105 74-75 0.32039480905227435 1105 76-77 0.36498147429097827 1105 78-79 0.2968610929458073 1105 80-81 0.2985133186089044 1105 82-83 0.3176230462700893 1105 84-85 0.2993196586382325 1105 86-87 0.27557287243665485 1105 88-89 0.3122225197287918 1105 90-91 0.2655911128396582 1105 92-93 0.24802127847023314 1105 94-95 0.21216564672011629 1105 96-97 0.24217293517260785 1105 98-99 0.15322022470622443 1105 100 0.22049125027557537 1106 1 1.6138015156595138 1106 2 1.2920553490607105 1106 3 1.1669785458422055 1106 4 0.6393126309280319 1106 5 0.9697292206355712 1106 6 0.6523464668679182 1106 7 0.3775617448789603 1106 8 0.2849521796859946 1106 9 0.25417059100111317 1106 10-11 0.19099251683523732 1106 12-13 0.2035683399600785 1106 14-15 0.3225164174133255 1106 16-17 0.3198741953743962 1106 18-19 0.3114358986830368 1106 20-21 0.3537425962148788 1106 22-23 0.36769211110159006 1106 24-25 0.3719533125118204 1106 26-27 0.3386639267691791 1106 28-29 0.4113826857503753 1106 30-31 0.39852102565691894 1106 32-33 0.3493847313616385 1106 34-35 0.3253448751877883 1106 36-37 0.33943089104268154 1106 38-39 0.33675136797047145 1106 40-41 0.30084765779907485 1106 42-43 0.36467102226645665 1106 44-45 0.3393010464166224 1106 46-47 0.34992320126251997 1106 48-49 0.34485881809401775 1106 50-51 0.3209671567386607 1106 52-53 0.39417011872019714 1106 54-55 0.47542384553374006 1106 56-57 0.49247172236381687 1106 58-59 0.49900536650986993 1106 60-61 0.45813535543533845 1106 62-63 0.5018695712064627 1106 64-65 0.45909882693767656 1106 66-67 0.32815689483439314 1106 68-69 0.38829951237028837 1106 70-71 0.38889035520586646 1106 72-73 0.406842321413464 1106 74-75 0.473539779161527 1106 76-77 0.49627850067729185 1106 78-79 0.44365964822650383 1106 80-81 0.5089542500319801 1106 82-83 0.5662251386221833 1106 84-85 0.5655491302248485 1106 86-87 0.5479286581852989 1106 88-89 0.5115993664270668 1106 90-91 0.4830033302518757 1106 92-93 0.51383846393027 1106 94-95 0.5025449929030366 1106 96-97 0.45474830623011897 1106 98-99 0.5230430275647429 1106 100 0.5652934388277657 1107 1 1.7076743482437955 1107 2 1.3260433568923276 1107 3 1.2282205547714398 1107 4 0.6526517860623429 1107 5 0.9928894202825518 1107 6 0.6134414475113559 1107 7 0.39565104463125067 1107 8 0.3215692683405251 1107 9 0.327048785575343 1107 10-11 0.20894971361952486 1107 12-13 0.14949743635722967 1107 14-15 0.2387673073701606 1107 16-17 0.21911902991658394 1107 18-19 0.22116739128897223 1107 20-21 0.23881255053042594 1107 22-23 0.2536186488728447 1107 24-25 0.2911517209723442 1107 26-27 0.2886701379262675 1107 28-29 0.2521170299263531 1107 30-31 0.24013188461358936 1107 32-33 0.2795495402434085 1107 34-35 0.23650586096330528 1107 36-37 0.22625665603474943 1107 38-39 0.2098110766579282 1107 40-41 0.22840980645153763 1107 42-43 0.24496394785123243 1107 44-45 0.2782170975298115 1107 46-47 0.22429188813120504 1107 48-49 0.23288138962430338 1107 50-51 0.26995594328251826 1107 52-53 0.2934942150431894 1107 54-55 0.2614388227656832 1107 56-57 0.23421320102710297 1107 58-59 0.18613455116108213 1107 60-61 0.2111162473204118 1107 62-63 0.21897083346191692 1107 64-65 0.24978283293005177 1107 66-67 0.18265034252717527 1107 68-69 0.17108700551020206 1107 70-71 0.1533377847138624 1107 72-73 0.11255074559655753 1107 74-75 0.06961479139843618 1107 76-77 0.10844782609000703 1107 78-79 0.2081844854385686 1107 80-81 0.19535802715712336 1107 82-83 0.1806527295090241 1107 84-85 0.13796565968983643 1107 86-87 0.16066857571596316 1107 88-89 0.07786731388994639 1107 90-91 0.07979911482298974 1107 92-93 0.058708048172103844 1107 94-95 0.06693707822023143 1107 96-97 0.09892548551192348 1107 98-99 0.10435912540066283 1107 100 0.06833783284132267 1108 1 0.8429933799654847 1108 2 0.9836186555099076 1108 3 1.011649070491984 1108 4 0.5626710172013603 1108 5 0.8842412396600352 1108 6 0.6206479613623586 1108 7 0.3897620177120089 1108 8 0.31096500004528593 1108 9 0.34059774537432475 1108 10-11 0.24668584859184506 1108 12-13 0.20046422780513495 1108 14-15 0.2673337744454116 1108 16-17 0.24559043203461783 1108 18-19 0.27795185352886165 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2215 50-51 -0.9259273491633238 2215 52-53 -0.9009029636786892 2215 54-55 -0.9339043245651624 2215 56-57 -0.867797995549509 2215 58-59 -0.8094658457257893 2215 60-61 -0.8673350322163884 2215 62-63 -0.8601216301797052 2215 64-65 -0.8881468567288096 2215 66-67 -0.8296016474202474 2215 68-69 -0.8015713810186256 2215 70-71 -0.8355077013854313 2215 72-73 -0.8767924485613818 2215 74-75 -0.8339610019328951 2215 76-77 -0.7285288436703325 2215 78-79 -0.7555892422188535 2215 80-81 -0.7714431267496593 2215 82-83 -0.8172078915435144 2215 84-85 -0.7833746189368362 2215 86-87 -0.7355131986335159 2215 88-89 -0.6893079190952776 2215 90-91 -0.6591887583202265 2215 92-93 -0.6477672544820408 2215 94-95 -0.7024774156373894 2215 96-97 -0.7696011107816041 2215 98-99 -0.7358093759171673 2215 100 -0.7988762434928347 2216 1 -6.260856166904361 2216 2 -6.271018571967758 2216 3 -5.672866552465173 2216 4 -3.0801405240908863 2216 5 -4.013907517474088 2216 6 -4.387983085913277 2216 7 -2.1519015697632753 2216 8 -1.32022719302239 2216 9 -1.0156707951362094 2216 10-11 -0.9409419552225486 2216 12-13 -0.7801589637019504 2216 14-15 -1.043047466102685 2216 16-17 -0.959715300486863 2216 18-19 -0.9999007918848051 2216 20-21 -0.9478356052061656 2216 22-23 -0.9175281198995862 2216 24-25 -1.4141015461977773 2216 26-27 -1.309124197868563 2216 28-29 -1.9211314872497738 2216 30-31 -1.3822757395450722 2216 32-33 -1.1883395862828934 2216 34-35 -1.081734054013964 2216 36-37 -1.091027179345275 2216 38-39 -1.1195235833479984 2216 40-41 -1.1477603598369868 2216 42-43 -1.235944864193236 2216 44-45 -1.2261554609523628 2216 46-47 -1.2153071975026961 2216 48-49 -1.2823715367112882 2216 50-51 -1.295812340359987 2216 52-53 -1.2713342561047796 2216 54-55 -1.1284214660606011 2216 56-57 -1.1052444382754487 2216 58-59 -1.1516814663578145 2216 60-61 -1.0519166068047312 2216 62-63 -1.1708423969600474 2216 64-65 -1.2298172519687256 2216 66-67 -1.2389704960961865 2216 68-69 -1.23454075360344 2216 70-71 -1.2626771004597543 2216 72-73 -1.2796822163562425 2216 74-75 -1.2677970660800568 2216 76-77 -1.2774458071983688 2216 78-79 -1.2390165658810481 2216 80-81 -1.1993434676346943 2216 82-83 -1.1981747842037578 2216 84-85 -1.2076649577861467 2216 86-87 -1.1587630981921464 2216 88-89 -1.0660971262234895 2216 90-91 -1.0704331948964452 2216 92-93 -1.0557765286779492 2216 94-95 -1.063352538178247 2216 96-97 -1.1297444696915697 2216 98-99 -1.1366323854955844 2216 100 -1.1434656543607282 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3234.0 3 2110.0 4 2491.0 5 3344.0 6 2538.0 7 2126.0 8 2165.0 9 2437.0 10 2854.0 11 5938.0 12 4275.0 13 6240.0 14 5537.0 15 5785.0 16 6890.0 17 8989.0 18 12210.0 19 20515.0 20 46852.0 21 70175.0 22 32070.0 23 19266.0 24 19342.0 25 20915.0 26 20902.0 27 21755.0 28 24488.0 29 28740.0 30 33918.0 31 42188.0 32 54085.0 33 74784.0 34 110960.0 35 224743.0 36 365997.0 37 694766.0 38 951181.0 39 328135.0 40 1646.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 14.462226205596506 40.839950754452985 25.979004884369168 18.718818155581346 2 18.802307318293206 38.219112477202785 25.21832685954361 17.760253344960393 3 19.552264873032378 24.521403060805348 20.627940361213735 35.29839170494854 4 32.84106838208872 25.345062659417383 22.659167881963512 19.154701076530383 5 18.60377011706911 37.578782471523894 23.710480959533378 20.106966451873618 6 35.82643863659045 22.73186992938565 23.62611148840255 17.81557994562135 7 19.651608082064488 22.90909168358899 38.29931728547496 19.139982948871566 8 34.49397033882576 23.033415221752907 23.539472266966392 18.933142172454943 9 34.3701944814467 24.326489554814632 23.384661165111762 17.918654798626903 10-11 17.954649596876514 40.274619316214455 23.409352440496004 18.361378646413026 12-13 17.922077803532297 24.158168993600047 31.188366895008983 26.731386307858674 14-15 18.256255813408636 31.968141379663923 23.246895027449483 26.528707779477955 16-17 26.451357612690156 23.840267901750696 31.240716027430356 18.46765845812879 18-19 18.402807412130308 24.078332968973424 31.203037316388087 26.31582230250818 20-21 26.600277613304506 32.13062125865564 23.155943584010885 18.113157544028972 22-23 26.255186617833782 24.215302032536528 31.21698128420916 18.31253006542053 24-25 18.287884405519687 32.090281487237995 23.192136084646844 26.429698022595478 26-27 18.63537054787226 27.624111856619045 35.487318757892496 18.2531988376162 28-29 26.176689841931513 23.951027673396112 31.650149853189514 18.22213263148286 30-31 24.219040378506566 25.181479553777784 31.91175300432283 18.68772706339282 32-33 20.715018150062832 26.292275345429317 32.910977973461044 20.0817285310468 34-35 20.492408725446214 26.07459614955021 33.38292126049914 20.05007386450444 36-37 20.513918101673372 26.07434146834352 33.45040427517921 19.9613361548039 38-39 21.07074435951787 25.964538802043307 33.06650191959755 19.898214918841273 40-41 20.280796089959175 26.832070951796023 32.97720106386313 19.909931894381675 42-43 20.484436962413955 26.107730589088185 33.276959462389236 20.130872986108628 44-45 20.360657304052875 26.415856214205924 32.872505616442396 20.350980865298798 46-47 20.720691938259613 26.145993836671806 33.08801424965374 20.045299975414842 48-49 20.325077576022217 26.384900309391206 32.99729102019982 20.29273109438676 50-51 20.720354191539915 26.68124663274446 32.42824750258235 20.17015167313327 52-53 20.872502917608205 26.599276040651628 32.3150800826591 20.21314095908107 54-55 21.04632290726974 27.36178693741882 30.886933812832957 20.704956342478486 56-57 21.401913550926242 27.97650037990464 29.859333345707746 20.762252723461373 58-59 22.095299883694533 28.32185696801204 28.87330372646123 20.709539421832197 60-61 21.79393922058566 28.625518493065126 28.68885669150646 20.891685594842752 62-63 21.21512334982582 28.755271109769616 28.61591512307598 21.41369041732858 64-65 21.415740505747465 28.56691394721963 29.16395071917072 20.853394827862182 66-67 21.34590529947169 28.11533853403978 29.667836889008914 20.870919277479622 68-69 21.578827500370075 28.047333515187926 29.577224583475136 20.796614400966863 70-71 20.786510357805128 28.710464063993886 29.73014751481447 20.772878063386518 72-73 20.773181540453894 28.19604260943863 29.89008059863535 21.140695251472124 74-75 20.893779746481847 28.511602516254538 29.396682543628682 21.197935193634933 76-77 21.244193938615695 28.271093410168824 29.481684499890985 21.003028151324497 78-79 21.14904968606896 28.58450758230495 28.96546393383955 21.300978797786538 80-81 21.58579162158704 28.944323496731673 28.57196108284828 20.89792379883301 82-83 21.478643351602148 28.94776841894814 28.66280415245383 20.910784076995885 84-85 21.247373601980314 29.296756731262235 28.14051780929878 21.315351857458666 86-87 21.310147930038454 29.498123638984 28.161528330434294 21.03020010054325 88-89 21.956257967642205 29.166721191790373 27.919485615562273 20.95753522500515 90-91 21.54372417839518 29.638667952116975 27.786648723922763 21.030959145565078 92-93 21.22832441624546 29.757520740179665 27.65596179929903 21.358193044275843 94-95 21.42379632362562 29.927108468562768 27.55196431342598 21.097130894385632 96-97 21.40352654938686 29.47633064926564 27.79949203398957 21.320650767357925 98-99 21.80336619266975 29.63102555152468 27.247280682721108 21.31832757308446 100 21.229802062725327 30.26435154034901 27.215119957419887 21.290726439505775 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 47325.0 1 25823.5 2 3445.0 3 2232.5 4 1887.5 5 1753.5 6 1790.5 7 2072.5 8 1971.0 9 1832.5 10 1904.5 11 2650.5 12 24692.0 13 28521.5 14 10364.5 15 10085.5 16 11299.0 17 12549.5 18 13579.0 19 14786.5 20 16018.0 21 17335.5 22 19116.5 23 21557.5 24 23968.5 25 26465.5 26 29865.5 27 33464.5 28 36908.0 29 40295.0 30 44300.0 31 48935.5 32 53117.0 33 57671.0 34 62769.0 35 67185.5 36 71927.5 37 76753.5 38 80974.0 39 84899.0 40 88438.0 41 92216.5 42 96298.5 43 99896.0 44 102975.0 45 105770.5 46 108026.5 47 111510.0 48 113771.5 49 115694.5 50 128786.5 51 126551.0 52 106427.0 53 97293.0 54 92389.0 55 87998.0 56 81966.0 57 74711.5 58 67948.0 59 57999.0 60 47277.5 61 39537.0 62 32421.0 63 25069.0 64 19219.0 65 14451.0 66 10668.5 67 8105.0 68 6096.5 69 4584.5 70 3482.5 71 2581.0 72 1817.5 73 1253.0 74 919.0 75 696.5 76 499.0 77 326.0 78 216.0 79 129.0 80 61.0 81 35.5 82 22.0 83 10.0 84 4.0 85 1.5 86 1.0 87 2.0 88 2.0 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.055437466112251435 2 0.0 3 0.0 4 2.738403924315384E-4 5 0.00979740070699504 6 6.085342054034187E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.128013081051279E-5 16-17 2.4341368216136748E-4 18-19 1.5213355135085468E-5 20-21 0.0 22-23 9.128013081051279E-5 24-25 0.004001112400527477 26-27 0.0071807036237603395 28-29 0.0010953615697261536 30-31 0.0059788485680885885 32-33 0.0029209641859364094 34-35 0.025558436626943584 36-37 0.025223742813971703 38-39 0.02254619231019666 40-41 0.002221149849722478 42-43 0.005887568437278075 44-45 0.007697957698353245 46-47 0.0015213355135085466 48-49 0.008930239464295168 50-51 0.010390721557263373 52-53 0.0022972166253979053 54-55 0.011638216678340382 56-57 0.0071502769134901695 58-59 0.012535804631310423 60-61 0.007895731315109357 62-63 0.0011866417005366664 64-65 0.01338775251887521 66-67 0.005279034231874657 68-69 0.002358070045938247 70-71 0.008276065193486493 72-73 0.005415954428090426 74-75 0.008306491903756664 76-77 0.007834877894569015 78-79 0.01563932907886786 80-81 0.008260851838351407 82-83 0.011699070098880724 84-85 0.007332837175111195 86-87 0.013326899098334868 88-89 0.019442667862639228 90-91 0.0175866385361588 92-93 0.017434504984807944 94-95 0.005963635212953503 96-97 0.010421148267533544 98-99 0.017008531041025548 100 0.01658255709724316 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3286586.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.61307714806688 #Duplication Level Percentage of deduplicated Percentage of total 1 85.87271433251883 56.34373030413465 2 9.59147024281282 12.586517540101305 3 1.950907723889956 3.8401517698905376 4 0.7078368899042606 1.857734258621439 5 0.39437722445157775 1.2938151626690928 6 0.2716595388579601 1.0694650968657367 7 0.20165923493426388 0.92620380575534 8 0.15231602915037964 0.7995138697224852 9 0.12205120839303411 0.7207339817076241 >10 0.7080903080384462 7.854903211509064 >50 0.015038129228045743 0.6829851759691447 >100 0.008629618966114881 1.3058581816616222 >500 0.0021818198025732537 1.0348344844025206 >1k 9.284339581283745E-4 1.069831535480474 >5k 0.0 0.0 >10k+ 1.3926509371925617E-4 8.613721621508883 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 221733 6.746605748335812 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 45654 1.3891010306743838 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG 15413 0.46896688539414455 No Hit ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 4303 0.13092613429254552 No Hit TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 4163 0.1266663948547216 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.042671027017093E-4 0.0 0.0 0.0 0.0 2 3.3469381297188025E-4 0.0 0.0 0.0 0.0 3 3.3469381297188025E-4 0.0 0.0 0.0 0.0 4 3.3469381297188025E-4 6.0853420540341864E-5 0.0 0.0 0.0 5 3.6512052324205117E-4 6.0853420540341864E-5 0.0 0.0 0.0 6 3.6512052324205117E-4 6.0853420540341864E-5 0.0 0.0 0.0 7 3.6512052324205117E-4 6.0853420540341864E-5 0.0 0.0 0.0 8 3.6512052324205117E-4 9.128013081051279E-5 0.0 0.0 0.0 9 3.6512052324205117E-4 9.128013081051279E-5 0.0 0.0 0.0 10-11 3.803338783771366E-4 1.5213355135085466E-4 0.0 0.0 0.0 12-13 4.411872989174785E-4 1.9777361675611105E-4 0.0 0.0 0.0 14-15 4.868273643227349E-4 2.4341368216136745E-4 0.0 0.0 0.0 16-17 5.324674297279913E-4 2.586270372964529E-4 0.0 0.0 0.0 18-19 5.933208502683332E-4 2.890537475666239E-4 0.0 0.0 0.0 20-21 6.237475605385041E-4 3.3469381297188025E-4 0.0 0.0 0.0 22-23 7.302410464841023E-4 3.955472335122221E-4 0.0 0.0 0.0 24-25 8.82374597834957E-4 3.955472335122221E-4 0.0 0.0 0.0 26-27 9.584413735103844E-4 3.955472335122221E-4 0.0 0.0 0.0 28-29 0.001080148214591068 3.955472335122221E-4 0.0 0.0 0.0 30-31 0.0012018550556717519 3.955472335122221E-4 0.0 0.0 0.0 32-33 0.001399628672427863 4.868273643227349E-4 0.0 0.0 0.0 34-35 0.0015517622237787175 4.868273643227349E-4 0.0 0.0 0.0 36-37 0.0017799625508049997 4.868273643227349E-4 0.0 0.0 0.0 38-39 0.0020385895881014525 4.868273643227349E-4 0.0 0.0 0.0 40-41 0.0025710570178294435 5.020407194578204E-4 0.0 0.0 0.0 42-43 0.003088311092422349 5.324674297279913E-4 0.0 0.0 0.0 44-45 0.0035751384567450847 5.476807848630768E-4 0.0 0.0 0.0 46-47 0.003970685690257307 5.476807848630768E-4 0.0 0.0 0.0 48-49 0.004594433250795811 5.628941399981622E-4 0.0 0.0 0.0 50-51 0.00553766126917111 5.781074951332477E-4 0.0 0.0 0.0 52-53 0.006891649876193716 5.933208502683332E-4 0.0 0.0 0.0 54-55 0.008549905585918033 6.085342054034187E-4 0.0 0.0 0.0 56-57 0.00996474761348098 6.84600981078846E-4 0.0 0.0 0.0 58-59 0.012231537528608713 7.302410464841023E-4 0.0 0.0 0.0 60-61 0.01506122158373461 7.910944670244443E-4 0.0 0.0 0.0 62-63 0.01932096102155854 8.82374597834957E-4 0.0 0.0 0.0 64-65 0.02408274117884029 9.12801308105128E-4 0.0 0.0 0.0 66-67 0.03076140408314281 9.584413735103843E-4 0.0 0.0 0.0 68-69 0.03940258979987135 9.888680837805554E-4 0.0 0.0 0.0 70-71 0.050675685954969685 0.0010040814389156408 0.0 0.0 0.0 72-73 0.06412429189438523 0.0010649348594559825 0.0 0.0 0.0 74-75 0.08137623661757215 0.0010649348594559825 0.0 0.0 0.0 76-77 0.10380072208668814 0.0010953615697261536 0.0 0.0 0.0 78-79 0.13218884276875761 0.0010953615697261536 0.0 0.0 0.0 80-81 0.16964412311133803 0.0010953615697261536 0.0 0.0 0.0 82-83 0.2160752829836189 0.0010953615697261536 0.0 0.0 0.0 84-85 0.27352091197370154 0.0010953615697261536 0.0 0.0 0.0 86-87 0.34465856058536126 0.001110574924861239 0.0 0.0 0.0 88 0.4086611456386658 0.0011562149902664953 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGAGT 62280 0.0 76.49639 1 ACGAGTG 64645 0.0 75.29582 2 CGAGTGG 66495 0.0 73.79462 3 GTGGAAT 68125 0.0 73.430565 6 TGGAATC 68615 0.0 73.15955 7 GAATCAC 68855 0.0 72.97279 9 GGAATCA 69000 0.0 72.8535 8 AGTGGAA 68085 0.0 72.51301 5 GAGTGGA 68640 0.0 71.96092 4 TACGAGT 1350 0.0 70.706055 1 AACGTGT 1175 0.0 46.82118 1 ATCGAGT 750 0.0 44.513462 1 TCGAGTG 860 0.0 43.167667 2 AGTACTT 32015 0.0 41.387733 20-21 GAGTACT 31580 0.0 41.332817 20-21 ACGTGTG 1510 0.0 40.45725 2 GTACTTT 31680 0.0 40.395412 22-23 CATGGGG 17415 0.0 38.99575 24-25 CGTGTGG 1620 0.0 38.870464 3 TACTTTT 33755 0.0 38.66405 22-23 >>END_MODULE