FastQCFastQC Report
Thu 26 May 2016
SRR2132695_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132695_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5228826
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3075925.882620687703128No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1258862.4075385182065725No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG257130.49175474571156125No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT78540.15020580145524062No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA76690.14666772235297176No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT58280.11145905409742073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT19400.076.3435441
AACGAGT913450.072.607681
ACGAGTG952950.071.226222
GTGGAAT994100.070.238966
CGAGTGG975900.070.0038453
GAATCAC1007900.069.5995949
TGGAATC1009000.069.425267
GGAATCA1016900.068.959858
AGTGGAA996750.068.836515
GAGTGGA1009950.068.034544
AACGTGT18750.049.4001661
CGTGTGG23850.048.4695783
ATCGAGT8600.044.8310431
TCGAGTG11100.044.02842
ACGTGTG24500.042.5803682
GAGTACT451900.040.93056520-21
AGTACTT462700.040.84863320-21
GTACTTT459050.039.57796522-23
CATGGGG225850.038.34012624-25
TACTTTT507200.036.8121322-23
Produced by FastQC (version 0.11.5)