FastQCFastQC Report
Thu 26 May 2016
SRR2132694_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132694_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3701641
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2431336.568249054946171No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1181463.1917195643769887No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG354320.957197091776323No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT68580.18526918196551206No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC51520.13918151436079296No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT48760.13172536180575048No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT42360.11443573269260848No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39640.10708764031952314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT15800.069.334791
AACGAGT896600.060.9864541
GTGGAAT947950.060.6730776
TGGAATC960000.060.0909847
ACGAGTG931000.059.9403342
GGAATCA964750.059.824358
GAATCAC965300.059.795949
AGTGGAA956900.059.22985
CGAGTGG954650.059.055963
GAGTGGA978450.057.9460644
TCGAGTG9100.050.6080862
CGTGTGG16150.050.048513
ATCGAGT8350.046.735191
ACGTGTG16850.045.7382132
AACGTGT14400.044.731191
AGTACTT354200.040.61932420-21
GAGTACT351800.039.914620-21
GTACTTT352150.039.0824822-23
CATGGGG184650.037.6823824-25
TACTTTT375500.037.64092322-23