##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132694_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3701641
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.831668981405816	33.0	31.0	34.0	25.0	34.0
2	31.583562263331316	34.0	31.0	34.0	28.0	34.0
3	31.795256482192627	34.0	31.0	34.0	28.0	34.0
4	35.751680133216595	37.0	35.0	37.0	35.0	37.0
5	35.39247836297469	37.0	35.0	37.0	33.0	37.0
6	35.73960629893607	37.0	35.0	37.0	35.0	37.0
7	35.93851942962594	37.0	35.0	37.0	35.0	37.0
8	36.06402241600414	37.0	37.0	37.0	35.0	37.0
9	37.97027642604996	39.0	39.0	39.0	37.0	39.0
10-11	38.032548402181625	39.0	39.0	39.0	37.0	39.0
12-13	37.998567257062476	39.0	39.0	39.0	37.0	39.0
14-15	39.51041159853157	41.0	40.0	41.0	37.0	41.0
16-17	39.41937305643632	41.0	39.5	41.0	37.0	41.0
18-19	39.278245648348936	41.0	39.5	41.0	36.5	41.0
20-21	39.13112319644179	41.0	39.0	41.0	35.0	41.0
22-23	39.02728614147077	41.0	39.0	41.0	35.0	41.0
24-25	38.962586998577116	41.0	39.0	41.0	35.0	41.0
26-27	38.875042312315	41.0	39.0	41.0	35.0	41.0
28-29	38.69879561524199	41.0	39.0	41.0	35.0	41.0
30-31	38.59212008944141	41.0	39.0	41.0	35.0	41.0
32-33	38.44850108370855	41.0	38.5	41.0	35.0	41.0
34-35	38.34355735739906	41.0	38.0	41.0	35.0	41.0
36-37	38.19748200865508	40.5	38.0	41.0	35.0	41.0
38-39	37.79331085861649	40.0	36.5	41.0	34.5	41.0
40-41	37.59038342724213	40.0	35.5	41.0	34.0	41.0
42-43	37.47775229958821	40.0	35.0	41.0	33.0	41.0
44-45	37.31670332158089	40.0	35.0	41.0	33.0	41.0
46-47	37.20617734134672	40.0	35.0	41.0	33.0	41.0
48-49	37.04420255773047	40.0	35.0	41.0	33.0	41.0
50-51	36.786391765165774	40.0	35.0	41.0	33.0	41.0
52-53	36.318332058673434	39.5	35.0	41.0	31.5	41.0
54-55	35.50326517347307	39.0	35.0	41.0	28.0	41.0
56-57	34.8889684331895	39.0	35.0	41.0	23.5	41.0
58-59	34.577147135554206	39.0	35.0	41.0	22.0	41.0
60-61	34.33028324464745	38.0	35.0	41.0	21.0	41.0
62-63	34.064172079356155	37.5	35.0	40.0	20.0	41.0
64-65	33.748435356102874	37.0	35.0	40.0	19.0	41.0
66-67	33.41463677866114	36.5	34.0	40.0	17.5	41.0
68-69	33.11216376736696	36.0	34.0	39.0	15.0	41.0
70-71	32.770104394240285	35.5	34.0	39.0	11.0	41.0
72-73	32.42442189828781	35.0	34.0	38.5	7.5	40.5
74-75	32.07812724140456	35.0	34.0	37.5	4.0	40.0
76-77	31.69817764607643	35.0	33.0	37.0	2.0	39.0
78-79	31.359588895843764	35.0	33.0	36.5	2.0	39.0
80-81	31.037338169746878	35.0	33.0	36.0	2.0	39.0
82-83	30.706941596983608	35.0	33.0	36.0	2.0	37.0
84-85	30.396372987007652	35.0	32.0	35.0	2.0	37.0
86-87	30.274047645355125	35.0	32.5	35.0	2.0	37.0
88-89	30.33661354518172	35.0	33.0	35.0	2.0	36.0
90-91	30.21601581568823	35.0	33.0	35.0	2.0	36.0
92-93	30.079404647830515	35.0	33.0	35.0	2.0	36.0
94-95	29.9152389440251	35.0	33.0	35.0	2.0	36.0
96-97	29.73483949415948	35.0	33.0	35.0	2.0	35.0
98-99	29.594272513190774	35.0	33.0	35.0	2.0	35.0
100	29.505108950327706	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.9265913942058717
1101	2	1.4037308466504257
1101	3	1.3062575089685708
1101	4	0.6559213951950369
1101	5	0.981913373313418
1101	6	0.5924403388753916
1101	7	0.39064308310639007
1101	8	0.3142059045398611
1101	9	0.3354122257461185
1101	10-11	0.21596402827285033
1101	12-13	0.3156748085517762
1101	14-15	0.3945628862247901
1101	16-17	0.3920163497299569
1101	18-19	0.38273315133821484
1101	20-21	0.501997960769188
1101	22-23	0.4894049273850598
1101	24-25	0.5346831348876577
1101	26-27	0.5186521252158371
1101	28-29	0.5902102456727789
1101	30-31	0.5876697383856779
1101	32-33	0.6446516953042547
1101	34-35	0.5843971794123775
1101	36-37	0.5761486216888656
1101	38-39	0.6040506444829177
1101	40-41	0.56576860112758
1101	42-43	0.585581683173892
1101	44-45	0.5841548608253504
1101	46-47	0.5991957868816797
1101	48-49	0.4774040826267907
1101	50-51	0.4790589538739667
1101	52-53	0.4801091551303074
1101	54-55	0.3342423205436944
1101	56-57	0.39813837335778857
1101	58-59	0.3930877487193811
1101	60-61	0.4002917086996405
1101	62-63	0.38950952794515104
1101	64-65	0.41508431569209137
1101	66-67	0.3662787282704514
1101	68-69	0.3978531796014124
1101	70-71	0.38254427647344613
1101	72-73	0.4596384103838673
1101	74-75	0.5030462253995935
1101	76-77	0.44615955696416876
1101	78-79	0.4702439582286999
1101	80-81	0.453352205887839
1101	82-83	0.44406786491008887
1101	84-85	0.4364004269661841
1101	86-87	0.4707641171381951
1101	88-89	0.4167650540782901
1101	90-91	0.44345807077969823
1101	92-93	0.44184233881508916
1101	94-95	0.4434307755468616
1101	96-97	0.4511982224557265
1101	98-99	0.4950900170429726
1101	100	0.5691458090368648
1102	1	1.3588946551189451
1102	2	1.2118565983726057
1102	3	1.1629706241823854
1102	4	0.6247573455523394
1102	5	0.9245198675989101
1102	6	0.579343797302279
1102	7	0.33967614340591723
1102	8	0.27295045567687737
1102	9	0.2918046208819902
1102	10-11	0.25221059206516827
1102	12-13	0.26096535174769997
1102	14-15	0.40237784615214167
1102	16-17	0.39431792086771367
1102	18-19	0.39353488649538804
1102	20-21	0.5053449524171043
1102	22-23	0.48954584236165744
1102	24-25	0.48311549993436387
1102	26-27	0.4778732178834133
1102	28-29	0.5426770546756146
1102	30-31	0.5926721397615538
1102	32-33	0.5691299997574433
1102	34-35	0.5379738042594155
1102	36-37	0.5678947577781273
1102	38-39	0.5571769235322321
1102	40-41	0.5575535390188051
1102	42-43	0.5923006176543311
1102	44-45	0.6059155456833736
1102	46-47	0.4700553806844212
1102	48-49	0.61843450111175
1102	50-51	0.6571460567531773
1102	52-53	0.6077149129242798
1102	54-55	0.5592207085622718
1102	56-57	0.4831820471645898
1102	58-59	0.5063841125469111
1102	60-61	0.5708755106154229
1102	62-63	0.5363629065543449
1102	64-65	0.5008861201023365
1102	66-67	0.5044996393544281
1102	68-69	0.4924351111619458
1102	70-71	0.49913628429788304
1102	72-73	0.49207731902426843
1102	74-75	0.5205395350495721
1102	76-77	0.5656961257455109
1102	78-79	0.5885828099290187
1102	80-81	0.5725814515507004
1102	82-83	0.5154502332026638
1102	84-85	0.5263365001443354
1102	86-87	0.45230678444672634
1102	88-89	0.48035359968999813
1102	90-91	0.5692130649208842
1102	92-93	0.569932245942482
1102	94-95	0.5247452609600955
1102	96-97	0.5073581801442977
1102	98-99	0.5180432400555368
1102	100	0.5905293556355815
1103	1	1.8955919774484826
1103	2	1.3740298142131877
1103	3	1.2538298598371895
1103	4	0.6374697928654669
1103	5	0.91086060837943
1103	6	0.5407086279090692
1103	7	0.35870734306741525
1103	8	0.2849603850602449
1103	9	0.2812753807053596
1103	10-11	0.2219056419513663
1103	12-13	0.2586952972535954
1103	14-15	0.36235274392931416
1103	16-17	0.39921110513289193
1103	18-19	0.3429229946420804
1103	20-21	0.3814084623754681
1103	22-23	0.4449064190075802
1103	24-25	0.4155404574208177
1103	26-27	0.4016879478902027
1103	28-29	0.45056389243045203
1103	30-31	0.40115223637672415
1103	32-33	0.35947396346531235
1103	34-35	0.3241435274108113
1103	36-37	0.3673265600761866
1103	38-39	0.42259941115012367
1103	40-41	0.3908829764943107
1103	42-43	0.4388539917225387
1103	44-45	0.433309531889833
1103	46-47	0.4483147369423506
1103	48-49	0.47052357984824766
1103	50-51	0.4820265434174189
1103	52-53	0.461613472841492
1103	54-55	0.45312466766461057
1103	56-57	0.41685577844295807
1103	58-59	0.3797958068486338
1103	60-61	0.3709682925753697
1103	62-63	0.29775732330573135
1103	64-65	0.27339990077807386
1103	66-67	0.2539424856197243
1103	68-69	0.35678090081626124
1103	70-71	0.32980379794510384
1103	72-73	0.29155186388410215
1103	74-75	0.3202631318339755
1103	76-77	0.39615746822757814
1103	78-79	0.3761868668437067
1103	80-81	0.4088126845059925
1103	82-83	0.3998781367473292
1103	84-85	0.36540356881088343
1103	86-87	0.39321923014886906
1103	88-89	0.47753880950800465
1103	90-91	0.4122525265912529
1103	92-93	0.43509780436921375
1103	94-95	0.3884221936859511
1103	96-97	0.38936855254987535
1103	98-99	0.43776678629907906
1103	100	0.48862200682105694
1104	1	1.9350071659121824
1104	2	1.4305058099943793
1104	3	1.2694996027796108
1104	4	0.7141664103918899
1104	5	0.9619097948348312
1104	6	0.5717833278706976
1104	7	0.2763951116734731
1104	8	0.19655405624009603
1104	9	0.1803033245434733
1104	10-11	0.13357018185422476
1104	12-13	0.15468649428971304
1104	14-15	0.2790726881994203
1104	16-17	0.2488891799852766
1104	18-19	0.2612711578764362
1104	20-21	0.2725583547990098
1104	22-23	0.28843149554426617
1104	24-25	0.3131600074609082
1104	26-27	0.33158951293815164
1104	28-29	0.32349881881966525
1104	30-31	0.3774744426248091
1104	32-33	0.32741250126796473
1104	34-35	0.37461833445103565
1104	36-37	0.3788994359485045
1104	38-39	0.39008098834590044
1104	40-41	0.39657928228260886
1104	42-43	0.3780528017560627
1104	44-45	0.36371837989106837
1104	46-47	0.34852906647942206
1104	48-49	0.2602395343298838
1104	50-51	0.3154270434239379
1104	52-53	0.3887555810031387
1104	54-55	0.229072003069561
1104	56-57	0.27666660624938544
1104	58-59	0.2827087200835692
1104	60-61	0.24078020801850641
1104	62-63	0.32519431888548667
1104	64-65	0.33709458736636577
1104	66-67	0.2725473349475678
1104	68-69	0.2907511845898725
1104	70-71	0.3260735869971896
1104	72-73	0.27316270363920836
1104	74-75	0.3012849052486146
1104	76-77	0.4058729554849947
1104	78-79	0.3223711768416173
1104	80-81	0.30650172457468017
1104	82-83	0.36645483083724883
1104	84-85	0.35303478413767664
1104	86-87	0.3279059563087543
1104	88-89	0.2838983247590434
1104	90-91	0.2570292853266203
1104	92-93	0.2612507567284261
1104	94-95	0.29272754999037076
1104	96-97	0.3286823495279272
1104	98-99	0.33620093410890917
1104	100	0.34041105431937524
1105	1	1.654572119814965
1105	2	1.3033458126128394
1105	3	1.2155997354813621
1105	4	0.6194487174549721
1105	5	0.9867737787514557
1105	6	0.6159791162175452
1105	7	0.2923411786903287
1105	8	0.19884242335580637
1105	9	0.20613357229068896
1105	10-11	0.18413361877266965
1105	12-13	0.14725935877164886
1105	14-15	0.19059735005933476
1105	16-17	0.2494282751086061
1105	18-19	0.28421909870726836
1105	20-21	0.3314347118655476
1105	22-23	0.3151794152146934
1105	24-25	0.37374326070928987
1105	26-27	0.34615685334537005
1105	28-29	0.41131493897405846
1105	30-31	0.3234011297220789
1105	32-33	0.4006054644996695
1105	34-35	0.35901981875960587
1105	36-37	0.3022105691979604
1105	38-39	0.30308350430650677
1105	40-41	0.2719566259482278
1105	42-43	0.2982561799754819
1105	44-45	0.3614613132983351
1105	46-47	0.355618827467751
1105	48-49	0.3518775271870638
1105	50-51	0.32955988049636886
1105	52-53	0.29951194062945774
1105	54-55	0.26338982678288403
1105	56-57	0.2812047420034318
1105	58-59	0.33233682696302935
1105	60-61	0.2973978665376791
1105	62-63	0.4155747348432186
1105	64-65	0.3096564425723827
1105	66-67	0.3908620649150052
1105	68-69	0.4356872568931607
1105	70-71	0.40250106595919277
1105	72-73	0.40851650681778295
1105	74-75	0.3737665929757483
1105	76-77	0.45009195792797385
1105	78-79	0.4181817842899669
1105	80-81	0.3991213289705442
1105	82-83	0.44558774217278696
1105	84-85	0.4451180255589584
1105	86-87	0.4367434673542405
1105	88-89	0.3817793141929613
1105	90-91	0.38217988815049253
1105	92-93	0.4316648679650221
1105	94-95	0.4757331629974537
1105	96-97	0.42594602003592286
1105	98-99	0.3890640518060273
1105	100	0.424222779564964
1106	1	1.686646358073503
1106	2	1.3202666768482345
1106	3	1.1773957725729716
1106	4	0.6573676491021985
1106	5	1.0220547774801076
1106	6	0.6611767187793163
1106	7	0.4222465576973988
1106	8	0.2951258281251441
1106	9	0.2591165719296171
1106	10-11	0.20307180781360046
1106	12-13	0.2078275314358109
1106	14-15	0.33479548722232266
1106	16-17	0.3531665634835335
1106	18-19	0.3574339545874068
1106	20-21	0.3554390863438428
1106	22-23	0.3602748680486698
1106	24-25	0.38353834341545223
1106	26-27	0.37483668614162013
1106	28-29	0.43982259919803113
1106	30-31	0.43274519756277385
1106	32-33	0.3476866480575751
1106	34-35	0.38227996454755697
1106	36-37	0.4522857206997699
1106	38-39	0.39795981862859264
1106	40-41	0.3872843919626163
1106	42-43	0.37882091165813847
1106	44-45	0.3684265133383775
1106	46-47	0.3887941480511756
1106	48-49	0.3931718281037888
1106	50-51	0.37083073461029414
1106	52-53	0.4415413102816572
1106	54-55	0.44340599854911744
1106	56-57	0.2326221197574725
1106	58-59	0.15365408845691064
1106	60-61	0.20192844866657822
1106	62-63	0.24818651002165382
1106	64-65	0.2218808398722274
1106	66-67	0.18811182728016007
1106	68-69	0.24435947591798168
1106	70-71	0.29563909536427246
1106	72-73	0.2551892361325443
1106	74-75	0.2411447898344008
1106	76-77	0.2268364238738485
1106	78-79	0.21551203146654885
1106	80-81	0.29873727422195984
1106	82-83	0.2750640499812853
1106	84-85	0.2103497761219515
1106	86-87	0.16891542003115134
1106	88-89	0.1517980232949192
1106	90-91	0.14656169652247186
1106	92-93	0.19259085754095295
1106	94-95	0.18578049025423837
1106	96-97	0.21251219112997788
1106	98-99	0.1653008918248382
1106	100	0.1796938678222908
1107	1	1.6704419164661601
1107	2	1.3081657725707316
1107	3	1.2274753196336192
1107	4	0.6652864547520068
1107	5	1.0154618227800398
1107	6	0.6721105598426504
1107	7	0.40814935056319257
1107	8	0.34826909928836614
1107	9	0.30040085033193975
1107	10-11	0.19090045146927537
1107	12-13	0.14944927706984856
1107	14-15	0.2442001404489602
1107	16-17	0.2545283414905555
1107	18-19	0.21322908084297865
1107	20-21	0.31246009521343865
1107	22-23	0.2869090396222802
1107	24-25	0.2988729259589036
1107	26-27	0.29094937188297365
1107	28-29	0.3157422892369084
1107	30-31	0.31217863239859156
1107	32-33	0.31788667264836334
1107	34-35	0.2915591827897117
1107	36-37	0.2829146560080247
1107	38-39	0.22445034857866375
1107	40-41	0.21118769422799488
1107	42-43	0.20765637479414067
1107	44-45	0.2678622740415264
1107	46-47	0.24567239782135175
1107	48-49	0.2559072239281761
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2214	2	-3.9204210870670835
2214	3	-3.422763377537631
2214	4	-1.8546327270322251
2214	5	-2.806838791943953
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2214	96-97	-0.8810168680087074
2214	98-99	-0.968751054259851
2214	100	-0.9690349507176244
2215	1	-3.6319549970693537
2215	2	-3.5862820391377532
2215	3	-3.623334472970793
2215	4	-1.918567536324467
2215	5	-2.7518561465691604
2215	6	-1.4061985541345337
2215	7	-0.9307869689524892
2215	8	-0.6507350065005966
2215	9	-0.5347904482510089
2215	10-11	-0.4565160515390261
2215	12-13	-0.3904650813041215
2215	14-15	-0.5536055139560503
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2215	22-23	-0.834913108681647
2215	24-25	-0.8688496866541868
2215	26-27	-0.9824613563602327
2215	28-29	-1.03222336614148
2215	30-31	-0.9770219694880424
2215	32-33	-1.006121974370835
2215	34-35	-1.1020037216207044
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2215	84-85	-0.8739527864452228
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2215	90-91	-0.9335716089295367
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2215	96-97	-0.9233916186155149
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2215	100	-0.9545532331460969
2216	1	-6.364602072403027
2216	2	-6.328305211626709
2216	3	-5.541863419981208
2216	4	-3.0232132135629044
2216	5	-3.9913277857679645
2216	6	-4.345380747191914
2216	7	-2.0369096998295433
2216	8	-1.2719893993329947
2216	9	-0.9492730853972304
2216	10-11	-0.8270847779271264
2216	12-13	-0.6796969341689305
2216	14-15	-0.9255105781460529
2216	16-17	-0.827849471558963
2216	18-19	-0.8115711643382753
2216	20-21	-0.817471151384261
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2216	24-25	-1.4805857006127425
2216	26-27	-1.3445731700988688
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2216	30-31	-1.5291257507273244
2216	32-33	-1.2305455958028588
2216	34-35	-1.2683852040334571
2216	36-37	-1.2952463505886627
2216	38-39	-1.2125485610960212
2216	40-41	-1.2750800774954527
2216	42-43	-1.3157770745967952
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2216	50-51	-1.347974764470436
2216	52-53	-1.391483802856996
2216	54-55	-1.3354659393134511
2216	56-57	-1.3314603002353564
2216	58-59	-1.2520745305814032
2216	60-61	-1.262505558085202
2216	62-63	-1.2614001971499533
2216	64-65	-1.3181118567694199
2216	66-67	-1.3577485932784583
2216	68-69	-1.425355417784722
2216	70-71	-1.3705503620051864
2216	72-73	-1.4035394922712285
2216	74-75	-1.3728805581787569
2216	76-77	-1.4552987285852979
2216	78-79	-1.4275937708376851
2216	80-81	-1.399967640337671
2216	82-83	-1.3597193227590303
2216	84-85	-1.4112286274934078
2216	86-87	-1.3719451704753993
2216	88-89	-1.3868028467328344
2216	90-91	-1.3726809884811182
2216	92-93	-1.4018203268763898
2216	94-95	-1.4233316600602741
2216	96-97	-1.4815834654293738
2216	98-99	-1.5881727382730055
2216	100	-1.6175782359611262
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5009.0
3	4666.0
4	6052.0
5	7937.0
6	5680.0
7	4250.0
8	3935.0
9	4379.0
10	4624.0
11	9083.0
12	6338.0
13	8733.0
14	8023.0
15	8289.0
16	9687.0
17	12151.0
18	16052.0
19	25986.0
20	56978.0
21	81886.0
22	36487.0
23	22423.0
24	22394.0
25	24380.0
26	24933.0
27	26471.0
28	30721.0
29	35714.0
30	42152.0
31	51156.0
32	65937.0
33	89520.0
34	132726.0
35	309487.0
36	407170.0
37	752033.0
38	975769.0
39	360073.0
40	2357.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	13.837894967313282	42.285786109800874	26.30728622535657	17.56903269752927
2	17.959985303811283	39.34442571400785	24.72209615197588	17.97349283020499
3	19.363087884535535	25.36377784879733	20.05958978734026	35.21354447932687
4	32.395503495621256	26.576933249154223	22.329643338301413	18.69791991692311
5	17.541368846276047	38.19207442934119	23.608502443442045	20.658054280940718
6	35.74681451444055	23.372528572451305	23.86332230643608	17.017334606672062
7	19.59579548638023	23.528699838801224	38.142947952002906	18.732556722815637
8	34.62569708948004	23.885352469350757	23.242961702661063	18.24598873850814
9	34.338851336474825	25.855721827157197	23.00031256407631	16.805114272291668
10-11	17.22159172107722	41.99405074668235	23.133132035224378	17.651225497016053
12-13	17.14430167593238	25.36269724697776	31.130071770871353	26.362929306218515
14-15	17.522470810537666	33.33092899071235	22.882790593509274	26.263809605240713
16-17	26.147788736768156	24.931621312079262	30.981634164530558	17.938955786622024
18-19	17.912582554602135	25.25587975711313	31.024726600985886	25.806811087298847
20-21	26.316990221363984	33.61594222670432	22.75070705127807	17.31636050065363
22-23	25.750062911312675	25.468998264147547	31.14542893763696	17.635509886902817
24-25	17.56779519640362	33.626039190895035	22.726369530169883	26.079796082531466
26-27	18.019383350365302	29.18453897844651	35.23047193980071	17.56560573138748
28-29	26.282143931004843	25.203027453315368	31.027465742451966	17.487362873227823
30-31	23.686784212259642	26.835811250195533	31.388606940724785	18.08879759682004
32-33	20.131864213089976	27.808854975030627	32.38149848643874	19.677782325440663
34-35	20.040173817220616	27.774869895145986	32.604226425005855	19.580729862627543
36-37	20.27684810968539	27.616634557820042	32.99592360188705	19.11059373060752
38-39	21.00870184595543	27.442517651410864	32.471975737999145	19.07680476463457
40-41	19.5027135936874	28.94116760307644	32.38676564865142	19.16935315458474
42-43	19.46395196224586	27.68511669298988	33.10393951297637	19.74699183178789
44-45	19.618720636800244	28.33052424629135	32.15706950420255	19.893685612705852
46-47	20.28274043094419	27.57160228042359	32.75747765894306	19.388179629689166
48-49	19.561352128140715	27.83360792109596	32.648084216760246	19.956955734003078
50-51	20.227171042108857	28.57003715487466	31.902925595148034	19.299866207868448
52-53	20.44277247783875	28.116342760658508	31.91241536513297	19.528469396369776
54-55	20.198780348430965	29.119957981433693	30.28823461230767	20.393027057827673
56-57	20.505829369615952	29.725293481240072	29.546846836989328	20.222030312154647
58-59	21.79605141842415	29.779024519733788	28.2902110868022	20.13471297503987
60-61	21.248758900456725	30.355099686466176	28.049619666311393	20.34652174676571
62-63	20.43461487859706	30.25564087303892	27.888028930200164	21.42171531816385
64-65	20.900472665945085	30.153717752451005	28.543562594599386	20.402246987004524
66-67	20.972239088756	29.47170233899162	29.257488729740857	20.298569842511526
68-69	21.39987597109486	29.59937258826118	28.85677957367394	20.143971866970016
70-71	19.84667322560482	30.827108398498314	28.98532830466398	20.340890071232877
72-73	19.82351754835173	29.67442845624188	29.503616763671587	20.998437231734798
74-75	20.073314345523034	30.34142935956396	28.545125228229267	21.040131066683742
76-77	20.9965881445961	29.650734993651657	28.78428285182319	20.568394009929055
78-79	20.67512908429184	30.134690778612672	28.17247444657896	21.017705690516525
80-81	21.290760610771045	30.848333493861585	27.64743619272198	20.21346970264539
82-83	21.102608921665613	30.47554667015742	28.03269260930938	20.389151798867587
84-85	20.859847100229402	30.96671537824146	27.144437248928032	21.029000272601102
86-87	20.8245596283439	31.128103365333633	27.604900459004313	20.44243654731816
88-89	22.14053492905243	30.51724957861624	27.10246042721477	20.239755065116558
90-91	21.186904140443712	31.279445094726878	27.17655924577325	20.35709151905616
92-93	20.605460359113557	31.322341895519106	27.0022685748346	21.069929170532735
94-95	20.91818215834689	31.576872768672164	26.891592572184962	20.61335250079598
96-97	21.006370106497158	30.757124745611137	27.364318622852007	20.872186525039698
98-99	21.80977819376139	30.908750214975655	26.406697324142485	20.874774267120472
100	20.650896063545705	32.051858122393796	26.368399422753885	20.928846391306617
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	118525.0
1	63498.5
2	6325.5
3	3483.5
4	2796.5
5	2677.0
6	2693.0
7	3085.0
8	2837.5
9	2508.5
10	2631.5
11	3612.0
12	29576.5
13	33395.0
14	11089.5
15	10274.0
16	11338.0
17	12664.5
18	13718.5
19	15002.0
20	16613.0
21	18436.5
22	20529.0
23	23063.0
24	25971.0
25	29173.5
26	33361.0
27	37848.0
28	42048.0
29	45997.5
30	50274.5
31	54854.0
32	59330.0
33	64307.0
34	70359.0
35	76260.0
36	82262.5
37	87247.0
38	90503.0
39	93901.5
40	96646.0
41	99343.5
42	101345.0
43	103300.0
44	106788.5
45	109281.5
46	111845.5
47	117509.5
48	120430.5
49	131098.5
50	176121.0
51	175024.0
52	124419.0
53	106418.5
54	102843.5
55	101391.0
56	93513.5
57	80071.5
58	68269.5
59	56279.5
60	45675.5
61	37176.5
62	29718.5
63	22791.0
64	16977.0
65	12702.0
66	10023.5
67	7986.0
68	6113.0
69	4513.0
70	3232.5
71	2183.5
72	1556.0
73	1164.5
74	850.5
75	678.5
76	536.5
77	381.5
78	270.0
79	166.0
80	78.5
81	37.5
82	20.0
83	14.0
84	12.0
85	10.5
86	9.5
87	7.5
88	3.0
89	1.0
90	1.5
91	1.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.057433986710218515
2	2.7015045489284348E-5
3	0.0
4	2.701504548928435E-4
5	0.008239588874231726
6	0.002323293912078454
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.4858275019106391E-4
16-17	3.3768806861605435E-4
18-19	0.0
20-21	0.0
22-23	4.052256823392652E-5
24-25	0.003876659027712304
26-27	0.007253539713872848
28-29	0.0012967221834856487
30-31	0.005727189643728281
32-33	0.0028635948218641407
34-35	0.026258624215584385
36-37	0.02559675560109692
38-39	0.02362465728037916
40-41	0.00248538418501416
42-43	0.005916294962153272
44-45	0.007037419349958573
46-47	0.0012967221834856487
48-49	0.009050040238910257
50-51	0.0101846721494602
52-53	0.0021206810709088215
54-55	0.011346319105499426
56-57	0.006875329077022867
58-59	0.011846097447051186
60-61	0.007726303009935324
62-63	0.0011346319105499426
64-65	0.013696628063067165
66-67	0.00528144139315509
68-69	0.0022827713438445273
70-71	0.008928472534208478
72-73	0.005308456438644375
74-75	0.008617799511081707
76-77	0.007631750350722828
78-79	0.015425590974381363
80-81	0.00863130703382635
82-83	0.011819082401561902
84-85	0.007077941918192499
86-87	0.012899684221133275
88-89	0.019964118616581133
90-91	0.018505306160159777
92-93	0.0172626140676527
94-95	0.006199952939790758
96-97	0.009955044262801282
98-99	0.017303136635886625
100	0.016749328203356294
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3701641.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.97915025009574
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.66732078949433	43.2506244945809
2	14.152599413587039	15.561957791780376
3	3.7542037866099576	6.192088021605217
4	1.2220212259775427	2.687427543673021
5	0.573773516057629	1.5772790174429057
6	0.3328479027679339	1.09798169140245
7	0.2478138276890933	0.9537215566598999
8	0.16445676083960928	0.7233354371075953
9	0.13044224301352125	0.6454443309839898
>10	0.672096730534211	7.275879032639305
>50	0.05333031420418498	1.9131212224807939
>100	0.023625640885937296	2.38659512480944
>500	0.001674836430464764	0.6377698353834241
>1k	0.0035467124338956135	4.0275182647010945
>5k	9.851978983043372E-5	0.32526288637810097
>10k+	1.4777968474565057E-4	10.74399374837159
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	243133	6.568249054946171	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	118146	3.1917195643769887	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	35432	0.957197091776323	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6858	0.18526918196551206	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	5152	0.13918151436079296	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4876	0.13172536180575048	No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT	4236	0.11443573269260848	No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3964	0.10708764031952314	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.020720539890281095	0.0	0.0	0.0	0.0
2	0.021828156755341752	0.0	0.0	0.0	0.0
3	0.02255756298355243	0.0	0.0	0.0	0.0
4	0.022773683347466704	0.0	0.0	0.0	0.0
5	0.022854728483934557	0.0	0.0	0.0	0.0
6	0.02288174352942384	0.0	0.0	0.0	0.0
7	0.02288174352942384	0.0	0.0	0.0	0.0
8	0.02293577362040241	0.0	0.0	2.7015045489284348E-5	0.0
9	0.02309786389333812	0.0	0.0	2.7015045489284348E-5	0.0
10-11	0.023219431598039898	0.0	0.0	2.7015045489284348E-5	0.0
12-13	0.023286969211763108	0.0	0.0	2.7015045489284348E-5	0.0
14-15	0.023395029393720245	0.0	0.0	2.7015045489284348E-5	0.0
16-17	0.023476074530188098	0.0	0.0	2.7015045489284348E-5	0.0
18-19	0.02354361214391131	0.0	0.0	2.7015045489284348E-5	0.0
20-21	0.023719209939591656	0.0	0.0	2.7015045489284348E-5	0.0
22-23	0.023827270121548793	0.0	0.0	2.7015045489284348E-5	0.0
24-25	0.02388130021252736	0.0	0.0	2.7015045489284348E-5	0.0
26-27	0.02397585287173986	0.0	0.0	2.7015045489284348E-5	0.0
28-29	0.024083913053696997	0.0	0.0	2.7015045489284348E-5	0.0
30-31	0.02435406350858984	0.0	0.0	2.7015045489284348E-5	0.0
32-33	0.024705259099950534	0.0	0.0	2.7015045489284348E-5	0.0
34-35	0.025218544964246938	0.0	0.0	2.7015045489284348E-5	0.0
36-37	0.02590742862422369	0.0	0.0	2.7015045489284348E-5	0.0
38-39	0.027298703466921835	0.0	0.0	2.7015045489284348E-5	0.0
40-41	0.029095203991959244	0.0	0.0	2.7015045489284348E-5	0.0
42-43	0.0315805881769734	0.0	0.0	2.7015045489284348E-5	0.0
44-45	0.03375529933886079	0.0	0.0	2.7015045489284348E-5	0.0
46-47	0.036321728660342806	0.0	0.0	2.7015045489284348E-5	0.0
48-49	0.03994174475590691	0.0	0.0	2.7015045489284348E-5	0.0
50-51	0.04398049405655492	0.0	0.0	2.7015045489284348E-5	0.0
52-53	0.048937754903838594	0.0	0.0	2.7015045489284348E-5	0.0
54-55	0.05573203884439361	0.0	0.0	2.7015045489284348E-5	0.0
56-57	0.06357990955903071	0.0	0.0	2.7015045489284348E-5	0.0
58-59	0.07327831088968378	0.0	0.0	2.7015045489284348E-5	0.0
60-61	0.08424641935833324	0.0	0.0	2.7015045489284348E-5	0.0
62-63	0.09868596117235573	0.0	0.0	2.7015045489284348E-5	0.0
64-65	0.11775858328779046	0.0	0.0	2.7015045489284348E-5	0.0
66-67	0.13999196572547148	0.0	0.0	5.4030090978568696E-5	0.0
68-69	0.1692087374221325	0.0	0.0	5.4030090978568696E-5	0.0
70-71	0.20757010201691628	0.0	0.0	6.753761372321087E-5	0.0
72-73	0.25290134834793543	1.3507522744642174E-5	0.0	8.104513646785304E-5	0.0
74-75	0.30626957071201666	2.7015045489284348E-5	0.0	8.104513646785304E-5	0.0
76-77	0.37657622659787915	2.7015045489284348E-5	0.0	8.104513646785304E-5	0.0
78-79	0.46055249550131955	2.7015045489284348E-5	0.0	8.104513646785304E-5	0.0
80-81	0.5642632551346821	2.7015045489284348E-5	0.0	8.104513646785304E-5	0.0
82-83	0.6920714353444865	2.7015045489284348E-5	0.0	9.455265921249522E-5	0.0
84-85	0.8471918265439571	2.7015045489284348E-5	0.0	1.0806018195713739E-4	0.0
86-87	1.0331633996921905	2.7015045489284348E-5	0.0	1.0806018195713739E-4	0.0
88	1.1946593416271325	2.7015045489284348E-5	0.0	1.0806018195713739E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	1580	0.0	69.33479	1
AACGAGT	89660	0.0	60.986454	1
GTGGAAT	94795	0.0	60.673077	6
TGGAATC	96000	0.0	60.090984	7
ACGAGTG	93100	0.0	59.940334	2
GGAATCA	96475	0.0	59.82435	8
GAATCAC	96530	0.0	59.79594	9
AGTGGAA	95690	0.0	59.2298	5
CGAGTGG	95465	0.0	59.05596	3
GAGTGGA	97845	0.0	57.946064	4
TCGAGTG	910	0.0	50.608086	2
CGTGTGG	1615	0.0	50.04851	3
ATCGAGT	835	0.0	46.73519	1
ACGTGTG	1685	0.0	45.738213	2
AACGTGT	1440	0.0	44.73119	1
AGTACTT	35420	0.0	40.619324	20-21
GAGTACT	35180	0.0	39.9146	20-21
GTACTTT	35215	0.0	39.08248	22-23
CATGGGG	18465	0.0	37.68238	24-25
TACTTTT	37550	0.0	37.640923	22-23
>>END_MODULE