FastQCFastQC Report
Thu 26 May 2016
SRR2132693_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132693_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4417439
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2948146.673866917007795No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1604983.6332816367130363No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG385300.8722248343440623No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT82710.18723518310043444No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC60820.1376815842844689No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA56480.12785688721451502No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT55340.1252762064173382No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT46520.10530988656549643No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT46340.10490241065015271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT15900.073.634841
AACGAGT1046950.061.699081
GTGGAAT1108450.061.14856
TGGAATC1122300.060.5571677
ACGAGTG1088700.060.5059852
GGAATCA1129950.060.143028
GAATCAC1131950.060.049219
CGAGTGG1113000.059.67053
AGTGGAA1125650.059.3464165
GAGTGGA1140250.058.6112674
TCGAGTG11800.048.98282
AACGTGT16350.048.0263821
ACGTGTG19350.044.92742
ATCGAGT11600.044.5877761
CGTGTGG21100.043.8737033
AGTACTT445350.040.51431320-21
GAGTACT434750.040.29689420-21
GTACTTT438550.039.10296222-23
TACTTTT470650.037.5643322-23
CATGGGG220850.036.86765324-25
Produced by FastQC (version 0.11.5)