##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132693_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4417439
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.81095743483951	33.0	31.0	34.0	25.0	34.0
2	31.56912885497683	34.0	31.0	34.0	28.0	34.0
3	31.783850552322285	34.0	31.0	34.0	28.0	34.0
4	35.75039542141951	37.0	35.0	37.0	35.0	37.0
5	35.38634127149238	37.0	35.0	37.0	33.0	37.0
6	35.73597575427753	37.0	35.0	37.0	35.0	37.0
7	35.93155830787929	37.0	35.0	37.0	35.0	37.0
8	36.05723338794265	37.0	37.0	37.0	35.0	37.0
9	37.96072362289553	39.0	39.0	39.0	37.0	39.0
10-11	38.0175723988492	39.0	39.0	39.0	37.0	39.0
12-13	37.97821803538204	39.0	39.0	39.0	37.0	39.0
14-15	39.476139568650524	41.0	40.0	41.0	37.0	41.0
16-17	39.38142201850439	41.0	39.5	41.0	37.0	41.0
18-19	39.22367405639331	41.0	39.0	41.0	36.0	41.0
20-21	39.06900355613286	41.0	39.0	41.0	35.0	41.0
22-23	38.962287764471675	40.5	39.0	41.0	35.0	41.0
24-25	38.88048810634397	41.0	39.0	41.0	35.0	41.0
26-27	38.80307583194697	41.0	39.0	41.0	35.0	41.0
28-29	38.6419328031468	41.0	39.0	41.0	35.0	41.0
30-31	38.53854563696296	41.0	39.0	41.0	35.0	41.0
32-33	38.392424773720705	41.0	38.0	41.0	35.0	41.0
34-35	38.293719392616396	41.0	38.0	41.0	35.0	41.0
36-37	38.144836521794645	40.5	38.0	41.0	35.0	41.0
38-39	37.733374926060094	40.0	36.0	41.0	34.5	41.0
40-41	37.521491298465016	40.0	35.0	41.0	33.5	41.0
42-43	37.40416789003764	40.0	35.0	41.0	33.0	41.0
44-45	37.2396052328057	40.0	35.0	41.0	33.0	41.0
46-47	37.12824466846062	40.0	35.0	41.0	33.0	41.0
48-49	36.95900814929193	40.0	35.0	41.0	33.0	41.0
50-51	36.6981304099502	40.0	35.0	41.0	33.0	41.0
52-53	36.23235069007178	39.0	35.0	41.0	31.0	41.0
54-55	35.40354920577285	39.0	35.0	41.0	27.5	41.0
56-57	34.79016484438155	39.0	35.0	41.0	23.5	41.0
58-59	34.48042949772481	39.0	35.0	41.0	22.0	41.0
60-61	34.22047729464968	38.0	35.0	41.0	20.0	41.0
62-63	33.93708922749131	37.5	35.0	40.0	19.0	41.0
64-65	33.624046874218294	37.0	35.0	40.0	17.5	41.0
66-67	33.29295752584247	36.0	34.0	40.0	14.5	41.0
68-69	32.98847160085289	36.0	34.0	39.0	12.0	41.0
70-71	32.649776827704926	35.0	34.0	39.0	7.5	41.0
72-73	32.3095243646828	35.0	34.0	38.5	6.0	40.5
74-75	31.964895836705384	35.0	33.5	37.0	2.0	40.0
76-77	31.59463107470188	35.0	33.0	37.0	2.0	39.0
78-79	31.25940063914861	35.0	33.0	36.5	2.0	39.0
80-81	30.930194169064926	35.0	33.0	36.0	2.0	39.0
82-83	30.59746937082776	35.0	33.0	36.0	2.0	37.0
84-85	30.26315643521054	35.0	32.0	35.0	2.0	37.0
86-87	30.132778290769835	35.0	32.5	35.0	2.0	37.0
88-89	30.19298161219657	35.0	33.0	35.0	2.0	36.0
90-91	30.056663148036677	35.0	33.0	35.0	2.0	36.0
92-93	29.903622325062102	35.0	33.0	35.0	2.0	36.0
94-95	29.732100658322615	35.0	33.0	35.0	2.0	36.0
96-97	29.546584004895145	35.0	33.0	35.0	2.0	35.0
98-99	29.404355442146457	35.0	33.0	35.0	2.0	35.0
100	29.30827952576142	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.9161094409535835
1101	2	1.4217530528218596
1101	3	1.2656045152706454
1101	4	0.6498705269443548
1101	5	0.9497321146494428
1101	6	0.5875562995976864
1101	7	0.3390971074990077
1101	8	0.27444025156905383
1101	9	0.25912991536606
1101	10-11	0.1627938311682655
1101	12-13	0.2314436837499798
1101	14-15	0.32794953068364663
1101	16-17	0.33439347799976815
1101	18-19	0.3232862234169147
1101	20-21	0.4983470830908274
1101	22-23	0.5255453082136157
1101	24-25	0.5223129530628796
1101	26-27	0.5358074678029539
1101	28-29	0.5984676463200458
1101	30-31	0.645942960322941
1101	32-33	0.6103768457498049
1101	34-35	0.594762551700299
1101	36-37	0.6021736112578537
1101	38-39	0.6036682262021813
1101	40-41	0.528189564344892
1101	42-43	0.5591671464728378
1101	44-45	0.6007010724673236
1101	46-47	0.5526779855698507
1101	48-49	0.557424799074326
1101	50-51	0.5678495342528649
1101	52-53	0.5223072214575026
1101	54-55	0.2865966525466064
1101	56-57	0.3427343102096856
1101	58-59	0.34883967823026296
1101	60-61	0.299407652641662
1101	62-63	0.2693014524977073
1101	64-65	0.30717183360349054
1101	66-67	0.3083979100286598
1101	68-69	0.36446893241637923
1101	70-71	0.361281000166116
1101	72-73	0.4051131228375979
1101	74-75	0.37013030371740285
1101	76-77	0.3736868644489064
1101	78-79	0.3463130872455231
1101	80-81	0.3603950594934524
1101	82-83	0.3381979745768753
1101	84-85	0.34314344484965886
1101	86-87	0.42108435267271815
1101	88-89	0.4161588484619081
1101	90-91	0.3948501908381381
1101	92-93	0.3755807844299568
1101	94-95	0.28513667703898093
1101	96-97	0.26775164547764163
1101	98-99	0.34537606502575713
1101	100	0.43301747853426065
1102	1	1.33116221794765
1102	2	1.200440167245869
1102	3	1.097868655368707
1102	4	0.5404191242582215
1102	5	0.8742696094289428
1102	6	0.5013561696390454
1102	7	0.24699321806416208
1102	8	0.21705150244202542
1102	9	0.2067563990846466
1102	10-11	0.19323916540778185
1102	12-13	0.210332971958465
1102	14-15	0.32255203539887844
1102	16-17	0.3360905338222153
1102	18-19	0.34990484183654047
1102	20-21	0.3996416453622018
1102	22-23	0.38730967690235474
1102	24-25	0.4042154037397765
1102	26-27	0.3391397588234497
1102	28-29	0.389011977676077
1102	30-31	0.4132606104388117
1102	32-33	0.313627548538463
1102	34-35	0.33044194439727903
1102	36-37	0.3670342441892416
1102	38-39	0.3643434852689964
1102	40-41	0.3462145466065465
1102	42-43	0.38254152827732213
1102	44-45	0.39850128023749676
1102	46-47	0.3229470147245408
1102	48-49	0.39168860333191446
1102	50-51	0.3671705373944292
1102	52-53	0.37990843302386423
1102	54-55	0.34908060334657876
1102	56-57	0.24838418368012327
1102	58-59	0.20593446824256034
1102	60-61	0.2849878138948725
1102	62-63	0.21645786542972445
1102	64-65	0.23061036499929344
1102	66-67	0.23682553776784943
1102	68-69	0.19165231959205897
1102	70-71	0.24508211680561942
1102	72-73	0.2160079185092343
1102	74-75	0.23353693742204484
1102	76-77	0.2290546425163278
1102	78-79	0.29453614699934505
1102	80-81	0.27591616197947033
1102	82-83	0.23130457296647222
1102	84-85	0.2954146725605611
1102	86-87	0.25633979900048587
1102	88-89	0.26862308572996696
1102	90-91	0.320966333214006
1102	92-93	0.32733782259517596
1102	94-95	0.32031834032134654
1102	96-97	0.35909945039341906
1102	98-99	0.352651668991399
1102	100	0.35302378791737254
1103	1	1.8102219551763952
1103	2	1.292732878991206
1103	3	1.1812371747504002
1103	4	0.5780988738820625
1103	5	0.8229834525889927
1103	6	0.43986251365146245
1103	7	0.24486887958560288
1103	8	0.18012669663244196
1103	9	0.17144211216446337
1103	10-11	0.0998748074985869
1103	12-13	0.13236797444200477
1103	14-15	0.21912584941969016
1103	16-17	0.23569306965183046
1103	18-19	0.27577414658870225
1103	20-21	0.36737729516296014
1103	22-23	0.3765442255654321
1103	24-25	0.35523692688225594
1103	26-27	0.32877041222766934
1103	28-29	0.3485832220685694
1103	30-31	0.344294099350023
1103	32-33	0.34789860611131473
1103	34-35	0.31409665015230814
1103	36-37	0.37136056493336866
1103	38-39	0.3735537579314254
1103	40-41	0.3584143074474966
1103	42-43	0.3983449210888992
1103	44-45	0.36789151174187396
1103	46-47	0.36323258363227495
1103	48-49	0.3706036464855629
1103	50-51	0.3650159676660678
1103	52-53	0.3227050463869503
1103	54-55	0.25371027779426214
1103	56-57	0.2544826195325598
1103	58-59	0.1399833638137764
1103	60-61	0.1406066727011961
1103	62-63	0.14549913451916296
1103	64-65	0.09308771179985342
1103	66-67	0.06363655572129545
1103	68-69	0.15276923782013085
1103	70-71	0.11890348188583744
1103	72-73	0.17940550957828805
1103	74-75	0.10541582606659006
1103	76-77	0.1717373094697301
1103	78-79	0.19992344503966208
1103	80-81	0.2139382197935653
1103	82-83	0.16782661087763273
1103	84-85	0.19991917902661882
1103	86-87	0.18060540992379615
1103	88-89	0.1615343405702454
1103	90-91	0.20044242146781954
1103	92-93	0.20639611665161084
1103	94-95	0.2260081043886366
1103	96-97	0.23361108172023037
1103	98-99	0.27798860506344525
1103	100	0.27168299542093166
1104	1	1.88898119578716
1104	2	1.3694861246721324
1104	3	1.2456591866709914
1104	4	0.620495475389724
1104	5	0.9005533259701437
1104	6	0.5251583681466485
1104	7	0.2623512857114818
1104	8	0.14510826822282752
1104	9	0.17866158143527144
1104	10-11	0.1618229967040108
1104	12-13	0.18528186785759004
1104	14-15	0.2918584462314371
1104	16-17	0.25830887438692685
1104	18-19	0.2950680014156646
1104	20-21	0.336403750125875
1104	22-23	0.3617376142213473
1104	24-25	0.3509757827370379
1104	26-27	0.33524365181350646
1104	28-29	0.333363149723219
1104	30-31	0.3886609874264053
1104	32-33	0.3847826355414412
1104	34-35	0.3850405315139085
1104	36-37	0.4233104130227048
1104	38-39	0.4149932305972115
1104	40-41	0.43640284259723217
1104	42-43	0.44799031990572047
1104	44-45	0.4492266334242814
1104	46-47	0.45304431263863165
1104	48-49	0.3514870466764535
1104	50-51	0.4203901189088768
1104	52-53	0.497462918498897
1104	54-55	0.4747083597298314
1104	56-57	0.44154140546758214
1104	58-59	0.3583306897132701
1104	60-61	0.35053148998001404
1104	62-63	0.3789308570765684
1104	64-65	0.2900223527383403
1104	66-67	0.24192936792845643
1104	68-69	0.2672233550960357
1104	70-71	0.3070025553235709
1104	72-73	0.28313256172868506
1104	74-75	0.2925771797115715
1104	76-77	0.3186689318185323
1104	78-79	0.3131105320813141
1104	80-81	0.267423178906423
1104	82-83	0.30285437386032754
1104	84-85	0.29541491777523987
1104	86-87	0.2982807859634029
1104	88-89	0.2447051186489979
1104	90-91	0.236636679027189
1104	92-93	0.3211454981088053
1104	94-95	0.3328403095128998
1104	96-97	0.403251211141054
1104	98-99	0.4579613187283904
1104	100	0.4540878452383197
1105	1	1.6200071263751
1105	2	1.3086679282119995
1105	3	1.1879704359901808
1105	4	0.5972572217928658
1105	5	0.9670241440150136
1105	6	0.5906911699236304
1105	7	0.3300633326187139
1105	8	0.26297560002391407
1105	9	0.19445499703706304
1105	10-11	0.18920657856336476
1105	12-13	0.17603555228770063
1105	14-15	0.23909475953673365
1105	16-17	0.2527066200849788
1105	18-19	0.2714126073797658
1105	20-21	0.3053127275210272
1105	22-23	0.3680752644817957
1105	24-25	0.35256363266871915
1105	26-27	0.33868440723892945
1105	28-29	0.39774792927361347
1105	30-31	0.31457481472266124
1105	32-33	0.3562299998166196
1105	34-35	0.3329351118338977
1105	36-37	0.27630696626563633
1105	38-39	0.25959728037508967
1105	40-41	0.2824765977867827
1105	42-43	0.3022881314304655
1105	44-45	0.3157772889155339
1105	46-47	0.28205244502753857
1105	48-49	0.31384206870846043
1105	50-51	0.27346341305800337
1105	52-53	0.23523443036020097
1105	54-55	0.16741226431437894
1105	56-57	0.19599721556259198
1105	58-59	0.1801087241247501
1105	60-61	0.12278051927803801
1105	62-63	0.17099328115377688
1105	64-65	0.10206506657409875
1105	66-67	0.18373258546395022
1105	68-69	0.290077516124299
1105	70-71	0.3027157226764885
1105	72-73	0.3507499662609561
1105	74-75	0.34111555065618404
1105	76-77	0.3624652761213092
1105	78-79	0.3503358344311671
1105	80-81	0.3722557838759002
1105	82-83	0.42373719772700014
1105	84-85	0.4103756408662669
1105	86-87	0.3624297550535154
1105	88-89	0.3327855705400218
1105	90-91	0.271957699702714
1105	92-93	0.3075009572263774
1105	94-95	0.3252125247156705
1105	96-97	0.340115253055739
1105	98-99	0.26359281037137805
1105	100	0.3016256663594987
1106	1	1.6555322588747359
1106	2	1.3044198240266986
1106	3	1.1516311017946421
1106	4	0.6316020365436827
1106	5	1.0008902731135976
1106	6	0.6760259233076411
1106	7	0.3857717089129906
1106	8	0.3157248913639208
1106	9	0.25477710081579374
1106	10-11	0.18463448877268007
1106	12-13	0.16698238432812218
1106	14-15	0.2701983754797155
1106	16-17	0.3175934593043266
1106	18-19	0.33108773777411216
1106	20-21	0.35144655308909734
1106	22-23	0.3351253863853785
1106	24-25	0.35131104026032745
1106	26-27	0.31860772144759153
1106	28-29	0.3957188061952266
1106	30-31	0.38032962739332277
1106	32-33	0.366693133420668
1106	34-35	0.325858058965629
1106	36-37	0.3366689338245408
1106	38-39	0.32090416488688334
1106	40-41	0.3105129323659028
1106	42-43	0.39209186587826395
1106	44-45	0.4240720767903312
1106	46-47	0.39849738029854365
1106	48-49	0.38034767798334457
1106	50-51	0.4237470926366811
1106	52-53	0.43946744149744177
1106	54-55	0.41480863345327634
1106	56-57	0.31600607872469766
1106	58-59	0.29052308003286953
1106	60-61	0.3012570073962877
1106	62-63	0.3637110801074286
1106	64-65	0.38006523918574686
1106	66-67	0.27055842408221054
1106	68-69	0.42121400367143735
1106	70-71	0.42658325439867895
1106	72-73	0.45709454557403006
1106	74-75	0.4050410765408827
1106	76-77	0.4168000150429947
1106	78-79	0.3521198494642981
1106	80-81	0.38098377130220484
1106	82-83	0.4263471666334979
1106	84-85	0.43998554766115205
1106	86-87	0.4794962992084564
1106	88-89	0.4453513510128424
1106	90-91	0.4277213309412602
1106	92-93	0.39403478995908614
1106	94-95	0.38124703009423655
1106	96-97	0.36991611425629145
1106	98-99	0.3885556598166495
1106	100	0.4772164775167127
1107	1	1.699314914990154
1107	2	1.3000656484664965
1107	3	1.243335683875614
1107	4	0.6542649961827038
1107	5	1.021922875496827
1107	6	0.6719109689504847
1107	7	0.4467427960895094
1107	8	0.36447825996261685
1107	9	0.3189397747305165
1107	10-11	0.23978561554273625
1107	12-13	0.15646748639579755
1107	14-15	0.2679931535010027
1107	16-17	0.2573174455627836
1107	18-19	0.23479327858023424
1107	20-21	0.32138280393959207
1107	22-23	0.30583199208127354
1107	24-25	0.3429563104817106
1107	26-27	0.3584919162092177
1107	28-29	0.3512195446661792
1107	30-31	0.3450397495649824
1107	32-33	0.3473233357888361
1107	34-35	0.36860196964269676
1107	36-37	0.361892404592254
1107	38-39	0.3742207141655456
1107	40-41	0.36902157553447523
1107	42-43	0.361773390660737
1107	44-45	0.38641785658118266
1107	46-47	0.3868295553295127
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2216	2	-6.374063832775892
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2216	4	-3.0084370784207835
2216	5	-3.8766198500213207
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2216	62-63	-1.3990246895822267
2216	64-65	-1.4235715290022029
2216	66-67	-1.4336673419459771
2216	68-69	-1.422169267622472
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2216	80-81	-1.4850259754417898
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2216	84-85	-1.4775541984981153
2216	86-87	-1.5434895168638754
2216	88-89	-1.5634034316416496
2216	90-91	-1.5390393732842433
2216	92-93	-1.583416047484672
2216	94-95	-1.5975304743596261
2216	96-97	-1.6396548983901162
2216	98-99	-1.7371818945721387
2216	100	-1.7156132061524971
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5762.0
3	5945.0
4	7504.0
5	10024.0
6	7294.0
7	5319.0
8	4852.0
9	5443.0
10	5739.0
11	10280.0
12	7695.0
13	10178.0
14	9879.0
15	10504.0
16	12113.0
17	14950.0
18	19639.0
19	31314.0
20	67892.0
21	97365.0
22	44196.0
23	27347.0
24	28064.0
25	30536.0
26	31464.0
27	33932.0
28	38731.0
29	45026.0
30	52622.0
31	64185.0
32	81315.0
33	110344.0
34	161457.0
35	385170.0
36	491608.0
37	890538.0
38	1124041.0
39	424416.0
40	2756.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	13.6552362086035	42.31922549788597	26.28458735266478	17.740950940845753
2	17.79777830548424	39.422774145834275	24.8153285195336	17.964119029147884
3	19.34652634705312	25.87032893946017	19.928447229265643	34.854697484221056
4	31.948544683837305	27.111835515034212	22.219596258000983	18.7200235431275
5	17.480641399456513	38.316728280479936	23.601475109174064	20.60115521088949
6	35.366837565354714	23.777819330325855	23.902461882831474	16.95288122148796
7	19.532720202814346	24.012600966306497	37.74137005627016	18.713308774609
8	34.26034858659055	24.243526622552118	23.240751032442102	18.255373758415228
9	33.90020779007927	26.29634500895202	23.032530839701465	16.77091636126724
10-11	17.13547148019475	42.07536312329383	23.14486968580664	17.644295710704778
12-13	17.096942368643912	25.78145391481354	30.966268011850307	26.155335704692245
14-15	17.511151546830384	33.60132058491389	22.846927171448872	26.040600696806848
16-17	25.91986364894811	25.382378088934697	30.784172484352297	17.913585777764897
18-19	17.84609815777875	25.705742625987593	30.855615663283636	25.592543552950026
20-21	26.082171140337195	33.94678455095815	22.72094758976864	17.250096718936017
22-23	25.530259221492273	25.937474851093	30.935439169102185	17.596826758312538
24-25	17.513732213378	33.95802306837531	22.698711723864086	25.829532994382603
26-27	17.957761704491766	29.497395253794945	35.08653471519623	17.458308326517066
28-29	25.776740562941587	25.71575426233459	31.132136871459377	17.375368303264455
30-31	23.32957830912075	27.179410091431365	31.47590562603898	18.015105973408904
32-33	19.801007312615088	28.36199866977505	32.35662389089439	19.48037012671547
34-35	19.784352789480234	28.21512460505983	32.586952502568025	19.413570102891914
36-37	19.974302305558016	28.201628969808663	32.90534410829578	18.918724616337542
38-39	20.65804992841524	27.851984051418754	32.461247660164524	19.028718360001477
40-41	19.26734676852763	29.356940512800893	32.38307950155846	18.992633217113013
42-43	19.299470894919164	28.20270555245088	33.00777838688999	19.49004516573996
44-45	19.402752093251912	28.923433090478497	32.096061441071036	19.577753375198558
46-47	20.066736391254544	28.04123711340206	32.64078480909499	19.25124168624841
48-49	19.403865821983825	28.438802660377387	32.49578196270468	19.661549554934098
50-51	20.04205125639279	29.15619809460763	31.708060046767137	19.093690602232446
52-53	20.111037273403223	28.64843829852114	31.835737095955025	19.404787332120613
54-55	20.005884153492413	29.709915081635557	30.141672485163962	20.14252827970807
56-57	20.24682676927288	30.261572714420947	29.34967178471441	20.14192873159176
58-59	21.6028576652024	30.33417725244615	28.13942155033205	19.9235435320194
60-61	21.017866655377237	30.82834006209036	27.965869546859928	20.187923735672477
62-63	20.21484634759964	30.83098060517416	27.89262753488231	21.061545512343884
64-65	20.66448969204858	30.756630372939163	28.456441527220978	20.122438407791286
66-67	20.77311123743574	30.058967339198144	29.028595322230803	20.139326101135314
68-69	21.15073798530583	30.207429577660083	28.677308614331665	19.96452382270242
70-71	19.628573081249385	31.29385327185522	28.913464989885274	20.16410865701012
72-73	19.67866521778502	30.297360318753963	29.299861903468262	20.724112559992754
74-75	20.005469674323457	30.746723742054144	28.46775314562872	20.780053437993683
76-77	20.797953221901714	30.11521050574377	28.74360565788981	20.34323061446471
78-79	20.550087881022478	30.506227745316878	28.135613673983705	20.808070699676936
80-81	21.039635958185	31.05580050146307	27.6812822965424	20.223281243809534
82-83	21.036831060987087	30.812929082443485	27.889086727023333	20.261153129546095
84-85	20.804958053146187	31.31123168695017	27.104672098691978	20.779138161211662
86-87	20.837829818839133	31.55663998226801	27.347216375014423	20.258313823878442
88-89	21.97343684656574	30.969458418967307	26.869737572310086	20.187367162156868
90-91	21.202053951665253	31.68801652471541	26.907296627290734	20.202632896328605
92-93	20.697486363369297	31.784129670906154	26.67237003469907	20.84601393102547
94-95	20.988075235092683	32.02768544917882	26.577539949569797	20.406699366158698
96-97	20.94428919255547	31.23180120151265	27.0963051861602	20.727604419771676
98-99	21.657516004837536	31.393583212306257	26.24851091134207	20.700389871514137
100	20.762169340021533	32.37020106481371	26.171617722140905	20.69601187302385
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	158221.0
1	84879.5
2	8652.5
3	4769.0
4	3790.0
5	3637.5
6	3667.5
7	4208.0
8	3935.5
9	3437.0
10	3529.5
11	4941.5
12	38345.0
13	42874.5
14	13712.0
15	12435.5
16	13569.0
17	15044.5
18	16443.0
19	17697.5
20	19332.0
21	21365.5
22	23915.0
23	27544.5
24	31412.0
25	35896.0
26	42748.5
27	49478.0
28	53669.0
29	57130.0
30	61058.5
31	66391.0
32	72774.0
33	78834.0
34	85728.5
35	92037.0
36	98043.0
37	104233.5
38	108764.5
39	111222.0
40	113196.5
41	115457.0
42	116350.0
43	119022.0
44	123256.0
45	125925.0
46	128374.0
47	134196.0
48	138236.5
49	150821.0
50	201582.5
51	202505.5
52	146258.5
53	125421.5
54	122054.5
55	119786.0
56	110684.0
57	96174.0
58	85769.0
59	72054.0
60	57228.5
61	46691.0
62	37069.5
63	28222.0
64	20990.0
65	15534.5
66	11381.5
67	8542.0
68	6605.0
69	5008.5
70	3610.5
71	2570.5
72	1991.5
73	1457.0
74	1039.5
75	727.5
76	496.0
77	338.5
78	235.5
79	140.5
80	64.5
81	38.0
82	20.5
83	12.5
84	14.5
85	13.5
86	8.5
87	4.0
88	1.5
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.054828148164581335
2	0.0
3	0.0
4	3.1692571193399615E-4
5	0.009281395849495602
6	1.5846285596699808E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	5.659387713107074E-5
16-17	4.0747591534370933E-4
18-19	0.0
20-21	0.0
22-23	1.1318775426214147E-5
24-25	0.004063440378010879
26-27	0.007017640764252771
28-29	0.0012111089706049137
30-31	0.005568837509697361
32-33	0.00319189467019239
34-35	0.02502581246735948
36-37	0.024663611653720628
38-39	0.022547000649018583
40-41	0.0025014493691933267
42-43	0.005964994649614856
44-45	0.007436435455022696
46-47	0.0013356155002932696
48-49	0.00897578891298782
50-51	0.010152941557314091
52-53	0.0021166110047020456
54-55	0.011115037468542293
56-57	0.006621483624335276
58-59	0.012371421540852063
60-61	0.0074251166795964805
62-63	0.0011205587671952008
64-65	0.013718355816571548
66-67	0.005614112611402218
68-69	0.0024561742674884704
70-71	0.008274024836562543
72-73	0.005410374653730363
74-75	0.008081605654316901
76-77	0.007934461573776119
78-79	0.015212434172831815
80-81	0.0082400685102839
82-83	0.011963945625508354
84-85	0.006836540357433345
86-87	0.012756259905343344
88-89	0.019694669241612617
90-91	0.017702564766598927
92-93	0.016989481914747438
94-95	0.005546199958844932
96-97	0.009587002786003383
98-99	0.016468818245141587
100	0.01593683580010952
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4417439.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.24205755117003
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.02804312157723	38.445913035875265
2	15.982452078871862	16.37947458468738
3	4.909469889957694	7.547140159408455
4	1.7262730580866292	3.5383113356603735
5	0.7303527366707556	1.8712388482568694
6	0.3989912836685008	1.2267080592093915
7	0.2404233059680402	0.8623849416739813
8	0.16220280264800552	0.6649284278600138
9	0.11571757699884229	0.5336646066230963
>10	0.5549520478081702	5.631222040930081
>50	0.11244427852792854	3.807006403752766
>100	0.03318346420661939	3.1192473568052246
>500	0.0017280633134504125	0.611604716137366
>1k	0.0034561264977454593	3.968698507307901
>5k	1.7723725629463854E-4	0.5797722827105458
>10k+	1.3292794222097892E-4	11.212684693101286
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	294814	6.673866917007795	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	160498	3.6332816367130363	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	38530	0.8722248343440623	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8271	0.18723518310043444	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	6082	0.1376815842844689	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA	5648	0.12785688721451502	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5534	0.1252762064173382	No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4652	0.10530988656549643	No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT	4634	0.10490241065015271	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.001969466924161262	2.2637550852428294E-5	0.0	0.0	0.0
2	0.001969466924161262	2.2637550852428294E-5	0.0	0.0	0.0
3	0.001969466924161262	2.2637550852428294E-5	0.0	0.0	0.0
4	0.00199210447501369	2.2637550852428294E-5	0.0	0.0	0.0
5	0.002014742025866118	2.2637550852428294E-5	0.0	0.0	0.0
6	0.002014742025866118	9.055020340971318E-5	0.0	0.0	0.0
7	0.002014742025866118	9.055020340971318E-5	0.0	0.0	2.2637550852428294E-5
8	0.002014742025866118	1.1318775426214149E-4	0.0	0.0	2.2637550852428294E-5
9	0.002060017127570975	1.1318775426214149E-4	0.0	0.0	2.2637550852428294E-5
10-11	0.0021052922292758318	1.3582530511456977E-4	0.0	0.0	2.2637550852428294E-5
12-13	0.0021732048818331167	1.6978163139321222E-4	0.0	0.0	2.2637550852428294E-5
14-15	0.0022524363098166155	2.4901305937671125E-4	0.0	0.0	2.2637550852428294E-5
16-17	0.0023316677378001143	2.829693856553537E-4	0.0	0.0	2.2637550852428294E-5
18-19	0.002388261614931185	3.1692571193399615E-4	0.0	0.0	2.2637550852428294E-5
20-21	0.00259199957260304	3.3956326278642443E-4	0.0	0.0	2.2637550852428294E-5
22-23	0.0027278248777176098	3.961571399174952E-4	0.0	0.0	2.2637550852428294E-5
24-25	0.0029202440599632503	4.0747591534370933E-4	0.0	0.0	2.2637550852428294E-5
26-27	0.00305606936507782	4.301134661961376E-4	0.0	0.0	2.2637550852428294E-5
28-29	0.003282444873602103	4.980261187534225E-4	0.0	0.0	2.2637550852428294E-5
30-31	0.0035427767084050283	6.11213873015564E-4	0.0	0.0	2.2637550852428294E-5
32-33	0.0038597024203390244	7.470391781301338E-4	0.0	0.0	2.2637550852428294E-5
34-35	0.004301134661961376	7.809955044087763E-4	0.0	0.0	2.2637550852428294E-5
36-37	0.005172680369779865	8.036330552612045E-4	0.0	0.0	2.2637550852428294E-5
38-39	0.0066328023997614905	8.375893815398469E-4	0.0	0.0	2.2637550852428294E-5
40-41	0.009428539930036384	8.375893815398469E-4	0.0	0.0	2.2637550852428294E-5
42-43	0.012586478273950132	8.602269323922752E-4	0.0	0.0	2.2637550852428294E-5
44-45	0.01599342967724059	9.055020340971319E-4	0.0	0.0	2.2637550852428294E-5
46-47	0.019683350466186404	9.507771358019885E-4	0.0	0.0	2.2637550852428294E-5
48-49	0.02384865982303321	9.96052237506845E-4	0.0	0.0	2.2637550852428294E-5
50-51	0.028885514887698506	0.0010526461146379159	0.0	0.0	2.2637550852428294E-5
52-53	0.03575601157141049	0.0011092399917689865	0.0	0.0	2.2637550852428294E-5
54-55	0.04422245559021867	0.001143196318047629	0.0	0.0	2.2637550852428294E-5
56-57	0.05468100408404055	0.0011771526443262715	0.0	0.0	2.2637550852428294E-5
58-59	0.06749385786651496	0.0011997901951786996	0.0	0.0	2.2637550852428294E-5
60-61	0.08380421325568954	0.001222427746031128	0.0	0.0	2.2637550852428294E-5
62-63	0.10335173841676139	0.0012450652968835564	0.0	0.0	2.2637550852428294E-5
64-65	0.12766446803226938	0.0013129779494408412	0.0	0.0	2.2637550852428294E-5
66-67	0.15630096986059117	0.0013356155002932696	0.0	0.0	2.2637550852428294E-5
68-69	0.19416227366127747	0.001369571826571912	0.0	0.0	2.2637550852428294E-5
70-71	0.24239157575237597	0.0014261657037029827	0.0	0.0	2.2637550852428294E-5
72-73	0.2996193043073147	0.0014488032545554108	0.0	0.0	2.2637550852428294E-5
74-75	0.3655511711650121	0.0014714408054078392	0.0	0.0	2.2637550852428294E-5
76-77	0.4450542497587403	0.0015733097842437664	0.0	0.0	2.2637550852428294E-5
78-79	0.5443538665729171	0.001607266110522409	0.0	0.0	2.2637550852428294E-5
80-81	0.6643555236416394	0.0016412224368010514	0.0	0.0	2.2637550852428294E-5
82-83	0.8121447743817176	0.0016751787630796939	0.0	0.0	2.2637550852428294E-5
84-85	0.9940148579301266	0.0017317726402107645	0.0	0.0	2.2637550852428294E-5
86-87	1.2060834343156748	0.0017883665173418354	0.0	0.0	2.2637550852428294E-5
88	1.389402321118639	0.001833641619046692	0.0	0.0	2.2637550852428294E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	1590	0.0	73.63484	1
AACGAGT	104695	0.0	61.69908	1
GTGGAAT	110845	0.0	61.1485	6
TGGAATC	112230	0.0	60.557167	7
ACGAGTG	108870	0.0	60.505985	2
GGAATCA	112995	0.0	60.14302	8
GAATCAC	113195	0.0	60.04921	9
CGAGTGG	111300	0.0	59.6705	3
AGTGGAA	112565	0.0	59.346416	5
GAGTGGA	114025	0.0	58.611267	4
TCGAGTG	1180	0.0	48.9828	2
AACGTGT	1635	0.0	48.026382	1
ACGTGTG	1935	0.0	44.9274	2
ATCGAGT	1160	0.0	44.587776	1
CGTGTGG	2110	0.0	43.873703	3
AGTACTT	44535	0.0	40.514313	20-21
GAGTACT	43475	0.0	40.296894	20-21
GTACTTT	43855	0.0	39.102962	22-23
TACTTTT	47065	0.0	37.56433	22-23
CATGGGG	22085	0.0	36.867653	24-25
>>END_MODULE