FastQCFastQC Report
Thu 26 May 2016
SRR2132692_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132692_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3693495
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2180275.902999733314922No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA968442.622015191573293No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG210940.5711121850713213No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT61260.16585916591196145No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT42890.11612307584009184No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC38980.10553689662501234No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37600.10180059807851372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAGT691000.068.5548251
ACGAGTG723100.067.079022
TACGAGT15650.066.99131
GTGGAAT751350.066.3026
TGGAATC760600.065.7119147
CGAGTGG742600.065.6533
GAATCAC762300.065.620869
GGAATCA764550.065.440038
AGTGGAA758300.064.683285
GAGTGGA766350.063.955634
AACGTGT13100.046.6551931
ACGTGTG16200.042.354652
CGTGTGG17850.042.1254963
AGTACTT313500.040.7900520-21
GAGTACT309900.040.10377520-21
GTACTTT311500.039.14449322-23
TACTTTT331900.037.95627622-23
CATGGGG172750.037.6806624-25
ATCGAGT8000.036.435911
ACTTTTT341150.035.5711324-25
Produced by FastQC (version 0.11.5)