##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2132692_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3693495 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.8186387148216 33.0 31.0 34.0 25.0 34.0 2 31.573561085096905 34.0 31.0 34.0 28.0 34.0 3 31.787265719866955 34.0 31.0 34.0 28.0 34.0 4 35.76295893185181 37.0 35.0 37.0 35.0 37.0 5 35.40634331439463 37.0 35.0 37.0 33.0 37.0 6 35.76898682684016 37.0 35.0 37.0 35.0 37.0 7 35.975246751383175 37.0 35.0 37.0 35.0 37.0 8 36.099547718353485 37.0 37.0 37.0 35.0 37.0 9 38.01743226943586 39.0 39.0 39.0 37.0 39.0 10-11 38.078176496786924 39.0 39.0 39.0 37.0 39.0 12-13 38.04841593125211 39.0 39.0 39.0 37.0 39.0 14-15 39.56342366782681 41.0 40.0 41.0 37.0 41.0 16-17 39.48193973458743 41.0 40.0 41.0 37.0 41.0 18-19 39.37257881762396 41.0 40.0 41.0 36.5 41.0 20-21 39.23578250410519 41.0 39.5 41.0 36.0 41.0 22-23 39.14202929745404 41.0 39.0 41.0 35.0 41.0 24-25 39.079919561282736 41.0 39.0 41.0 35.0 41.0 26-27 39.00917978770785 41.0 39.0 41.0 35.0 41.0 28-29 38.856547389396766 41.0 39.0 41.0 35.0 41.0 30-31 38.769636888638 41.0 39.0 41.0 35.0 41.0 32-33 38.63442430543428 41.0 39.0 41.0 35.0 41.0 34-35 38.54012540967295 41.0 39.0 41.0 35.0 41.0 36-37 38.41592597255445 40.5 38.0 41.0 35.0 41.0 38-39 38.05156958382237 40.0 37.0 41.0 35.0 41.0 40-41 37.848886217525674 40.0 37.0 41.0 34.0 41.0 42-43 37.73718293919445 40.0 36.0 41.0 34.0 41.0 44-45 37.574938506753085 40.0 36.0 41.0 33.5 41.0 46-47 37.46240850468188 40.0 35.0 41.0 33.0 41.0 48-49 37.29949167387528 40.0 35.0 41.0 33.0 41.0 50-51 37.05309848801745 40.0 35.0 41.0 33.0 41.0 52-53 36.612622191176655 39.5 35.0 41.0 32.5 41.0 54-55 35.84065485400684 39.0 35.0 41.0 30.0 41.0 56-57 35.244624806585634 39.0 35.0 41.0 26.5 41.0 58-59 34.916947498236766 39.0 35.0 41.0 26.0 41.0 60-61 34.646973395117634 38.0 35.0 41.0 25.5 41.0 62-63 34.34568383062655 37.5 35.0 40.0 25.0 41.0 64-65 34.02523246951735 37.0 35.0 40.0 23.5 41.0 66-67 33.696574247426895 36.0 34.5 39.5 23.0 41.0 68-69 33.37764204364701 36.0 34.0 39.0 22.0 41.0 70-71 33.023953599504 35.0 34.0 39.0 21.5 41.0 72-73 32.673165254047994 35.0 34.0 37.5 20.0 40.0 74-75 32.32050578110976 35.0 34.0 37.0 18.5 39.5 76-77 31.95314221354029 35.0 33.5 37.0 16.0 39.0 78-79 31.6365733539642 35.0 33.0 36.0 11.5 39.0 80-81 31.33025413598773 35.0 33.0 36.0 7.0 37.5 82-83 31.03239831108476 35.0 33.0 35.5 3.5 37.0 84-85 30.73823695442934 35.0 33.0 35.0 2.0 37.0 86-87 30.624145964729884 35.0 33.0 35.0 2.0 36.0 88-89 30.72552257414725 35.0 33.0 35.0 2.0 36.0 90-91 30.624830411304195 35.0 33.0 35.0 2.0 36.0 92-93 30.495276560547666 35.0 33.0 35.0 2.0 36.0 94-95 30.352818400999595 35.0 33.0 35.0 2.0 35.5 96-97 30.199695275071445 35.0 33.0 35.0 2.0 35.0 98-99 30.072727998819545 35.0 33.0 35.0 2.0 35.0 100 29.982626482505054 35.0 33.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.9443669831966908 1101 2 1.4206061582943619 1101 3 1.2776223374711613 1101 4 0.6317886689554797 1101 5 0.9269037003064895 1101 6 0.5715420128441622 1101 7 0.39362088817625107 1101 8 0.3134858045062856 1101 9 0.3409906076677345 1101 10-11 0.21520119768392476 1101 12-13 0.2682245986577172 1101 14-15 0.33466304516563383 1101 16-17 0.3591974584143287 1101 18-19 0.32432186243310923 1101 20-21 0.44540406436004076 1101 22-23 0.43457189918407835 1101 24-25 0.4021283021776014 1101 26-27 0.3591235404009083 1101 28-29 0.40155314154163335 1101 30-31 0.44412159550492447 1101 32-33 0.41945291101878723 1101 34-35 0.39795293017846944 1101 36-37 0.4463026970892443 1101 38-39 0.41027318893073783 1101 40-41 0.36205942454210316 1101 42-43 0.3537252014724288 1101 44-45 0.41976996102548014 1101 46-47 0.2981270481895564 1101 48-49 0.20529409290705303 1101 50-51 0.24296438525170316 1101 52-53 0.2883746302270396 1101 54-55 0.17602809799636532 1101 56-57 0.10474980342580409 1101 58-59 0.005374155791209034 1101 60-61 -0.02747548106063391 1101 62-63 -0.008198509582975078 1101 64-65 0.04673329335680165 1101 66-67 0.01653279734534152 1101 68-69 0.07976155888341907 1101 70-71 0.10564590780353456 1101 72-73 0.041924735039827965 1101 74-75 0.052054215816681904 1101 76-77 0.09271327190795375 1101 78-79 0.162105785240108 1101 80-81 0.13075933050205535 1101 82-83 0.10538522652843696 1101 84-85 0.1359042623904969 1101 86-87 0.15283708315891786 1101 88-89 0.09725260756993492 1101 90-91 0.1109413147066114 1101 92-93 0.11443705528832737 1101 94-95 0.11251036868679165 1101 96-97 0.1702295182671918 1101 98-99 0.15437051600247287 1101 100 0.11563736797855384 1102 1 1.4168823560069832 1102 2 1.2026768899170506 1102 3 1.122117039809556 1102 4 0.5585622791172469 1102 5 0.8722116786509915 1102 6 0.5470726546917248 1102 7 0.3236931790130555 1102 8 0.2819573441835814 1102 9 0.2695384254226738 1102 10-11 0.21614581448324088 1102 12-13 0.2268003827496088 1102 14-15 0.30808966454847564 1102 16-17 0.28309707569813014 1102 18-19 0.32016161201605087 1102 20-21 0.35324472547599584 1102 22-23 0.3593456486373867 1102 24-25 0.34144066763278147 1102 26-27 0.3416626050492013 1102 28-29 0.38362915593105384 1102 30-31 0.3939678223900529 1102 32-33 0.3882004967411916 1102 34-35 0.3484754207701499 1102 36-37 0.4002303830099052 1102 38-39 0.30838727803280364 1102 40-41 0.2859588986060544 1102 42-43 0.2721006037974689 1102 44-45 0.2825851838395721 1102 46-47 0.26487295226531415 1102 48-49 0.32341573419680003 1102 50-51 0.38890447875871814 1102 52-53 0.26707980159328315 1102 54-55 0.10457677507044139 1102 56-57 0.03477642758809196 1102 58-59 0.06493935585379518 1102 60-61 0.1460776464682212 1102 62-63 0.1414335463274412 1102 64-65 0.10234423407720072 1102 66-67 0.10052256866496379 1102 68-69 0.1470581351267981 1102 70-71 0.10829490228556438 1102 72-73 0.13132999698649428 1102 74-75 0.15633703378767905 1102 76-77 0.169587880713145 1102 78-79 0.20435650248515103 1102 80-81 0.16377349224523385 1102 82-83 0.1934041816356391 1102 84-85 0.17585253628840647 1102 86-87 0.12417347010329038 1102 88-89 0.09528853136584203 1102 90-91 0.10828149689536914 1102 92-93 0.12903623282988264 1102 94-95 0.06611986100431366 1102 96-97 0.12152240893167843 1102 98-99 0.21225754761625382 1102 100 0.15344319556999864 1103 1 1.8496435051142832 1103 2 1.341615595442292 1103 3 1.2379178255622598 1103 4 0.6127291859283872 1103 5 0.8838546030343011 1103 6 0.5448253026847567 1103 7 0.32983655486797403 1103 8 0.2618494872968782 1103 9 0.28821918376689837 1103 10-11 0.17751453390484073 1103 12-13 0.23832307480115134 1103 14-15 0.3064156174417363 1103 16-17 0.3250561983062923 1103 18-19 0.3498527542071912 1103 20-21 0.38007922911338454 1103 22-23 0.38343530097222356 1103 24-25 0.3684903407161073 1103 26-27 0.3595006564209129 1103 28-29 0.4104610980274188 1103 30-31 0.4016008544221279 1103 32-33 0.33430511327849644 1103 34-35 0.3368986161644969 1103 36-37 0.39085906522512204 1103 38-39 0.39554651302857735 1103 40-41 0.3891676289782424 1103 42-43 0.3803066605612173 1103 44-45 0.4352129246884715 1103 46-47 0.3376813938237575 1103 48-49 0.3641222232205976 1103 50-51 0.35336141605219495 1103 52-53 0.3012265842977584 1103 54-55 0.24599657322102075 1103 56-57 0.20670626057562203 1103 58-59 0.17055109187418083 1103 60-61 0.14197830030157377 1103 62-63 0.08784442711331764 1103 64-65 0.08692779955018892 1103 66-67 0.15838826066526934 1103 68-69 0.2512620642105361 1103 70-71 0.16296587234114668 1103 72-73 0.2153045560812501 1103 74-75 0.11798896358864397 1103 76-77 0.1456398735920601 1103 78-79 0.10246984747350041 1103 80-81 0.1471905159266811 1103 82-83 0.1844551979270399 1103 84-85 0.19940222378039962 1103 86-87 0.1917136222588276 1103 88-89 0.18136767657960107 1103 90-91 0.22793528061164992 1103 92-93 0.27455433652106365 1103 94-95 0.2476409841119782 1103 96-97 0.2568279687564683 1103 98-99 0.23965607056765847 1103 100 0.15204323335272818 1104 1 1.9165132924601487 1104 2 1.383862099459634 1104 3 1.2465428536355425 1104 4 0.6111634226483886 1104 5 0.9526343664395753 1104 6 0.5839162937211668 1104 7 0.29428409564870606 1104 8 0.2327114095411531 1104 9 0.20164409328308608 1104 10-11 0.1743708231596912 1104 12-13 0.19550590021820113 1104 14-15 0.31485776815284794 1104 16-17 0.310708231728384 1104 18-19 0.2851508733356809 1104 20-21 0.29631077904868164 1104 22-23 0.33024896467649967 1104 24-25 0.39847756832890013 1104 26-27 0.32468371711877353 1104 28-29 0.36657167695572923 1104 30-31 0.3418056098446556 1104 32-33 0.32896878393424345 1104 34-35 0.40036515816289153 1104 36-37 0.42357459543961085 1104 38-39 0.3461988443711874 1104 40-41 0.33306086954371494 1104 42-43 0.3505046020068505 1104 44-45 0.3484858183522306 1104 46-47 0.42668216841709494 1104 48-49 0.33434067534782486 1104 50-51 0.3872884896935602 1104 52-53 0.2866373402978013 1104 54-55 0.23673987726647283 1104 56-57 0.2646090223751756 1104 58-59 0.1424512671125555 1104 60-61 0.13841360495203503 1104 62-63 0.15231541958585382 1104 64-65 0.09747292534139973 1104 66-67 0.025294906126106298 1104 68-69 0.03514315114679789 1104 70-71 0.007228076005716844 1104 72-73 0.023529568570992865 1104 74-75 0.05160024148432285 1104 76-77 0.15956689376051258 1104 78-79 0.07567388330340208 1104 80-81 0.04143293461347852 1104 82-83 0.09297755800752228 1104 84-85 0.08321727166921278 1104 86-87 0.031139277340205496 1104 88-89 0.026306656346982038 1104 90-91 0.03487533900407058 1104 92-93 0.02338377626243826 1104 94-95 0.0387181501087035 1104 96-97 0.09358769356958163 1104 98-99 0.10553806043224867 1104 100 0.1527244018733782 1105 1 1.603228838773834 1105 2 1.2937733652258103 1105 3 1.206131441372765 1105 4 0.618412483468795 1105 5 0.9773989636095237 1105 6 0.6634010764613762 1105 7 0.35629930757056627 1105 8 0.24964674226635708 1105 9 0.23234091294281 1105 10-11 0.2004547793506859 1105 12-13 0.14751751496654464 1105 14-15 0.2576099231948845 1105 16-17 0.28700899695068927 1105 18-19 0.28712353600325 1105 20-21 0.366693052130195 1105 22-23 0.3563594622548507 1105 24-25 0.43348602895216004 1105 26-27 0.37195874698154086 1105 28-29 0.4820673395888946 1105 30-31 0.4539726266401658 1105 32-33 0.47696756332697987 1105 34-35 0.4147128538959848 1105 36-37 0.4438188533068299 1105 38-39 0.39310273963857867 1105 40-41 0.40040415377201555 1105 42-43 0.435967795181881 1105 44-45 0.4952055697706399 1105 46-47 0.5378345331805718 1105 48-49 0.5028234006115468 1105 50-51 0.4528790044244744 1105 52-53 0.4346834938428401 1105 54-55 0.47510192373495386 1105 56-57 0.4643317019462003 1105 58-59 0.3919035359118723 1105 60-61 0.3725918242888753 1105 62-63 0.41808159678387113 1105 64-65 0.322412295322998 1105 66-67 0.2952376449363143 1105 68-69 0.3364277839504126 1105 70-71 0.3495241936679889 1105 72-73 0.39583954706505153 1105 74-75 0.38143339846817526 1105 76-77 0.4066947153027556 1105 78-79 0.38653795522077417 1105 80-81 0.40063747292382246 1105 82-83 0.35955510096898635 1105 84-85 0.34341272303886683 1105 86-87 0.314244023592682 1105 88-89 0.3018321313340202 1105 90-91 0.36237396596917293 1105 92-93 0.37893368415344497 1105 94-95 0.39977813898208225 1105 96-97 0.4489653048565785 1105 98-99 0.379643867747113 1105 100 0.3964913275681674 1106 1 1.722971844228372 1106 2 1.353902804850783 1106 3 1.1815416706490645 1106 4 0.66216997637909 1106 5 0.974384397292269 1106 6 0.6480983863731282 1106 7 0.3655582030483302 1106 8 0.2834367249766174 1106 9 0.2738769741438958 1106 10-11 0.2274133844643771 1106 12-13 0.20150566851700802 1106 14-15 0.3295956700460394 1106 16-17 0.36403123931059156 1106 18-19 0.3637686951170025 1106 20-21 0.39690430273411437 1106 22-23 0.4315031211495324 1106 24-25 0.4372010071143535 1106 26-27 0.38455365124737995 1106 28-29 0.48609546837627704 1106 30-31 0.46498992720241716 1106 32-33 0.41471523328276305 1106 34-35 0.38274186368306573 1106 36-37 0.4456977926452552 1106 38-39 0.44862054297848886 1106 40-41 0.43910531124249275 1106 42-43 0.4914581251105119 1106 44-45 0.47318296157308737 1106 46-47 0.4089492874842833 1106 48-49 0.4688701966566313 1106 50-51 0.45743362982238267 1106 52-53 0.4826527639070406 1106 54-55 0.45441111761951447 1106 56-57 0.4661738262017465 1106 58-59 0.46913004949042403 1106 60-61 0.4335958223107923 1106 62-63 0.40274282115282034 1106 64-65 0.3811622814439417 1106 66-67 0.3479040854099722 1106 68-69 0.4100528251249358 1106 70-71 0.4246176388536895 1106 72-73 0.4481164665241124 1106 74-75 0.42129537874026823 1106 76-77 0.4402776331897016 1106 78-79 0.39725655360989265 1106 80-81 0.43525356189440245 1106 82-83 0.4493146502569978 1106 84-85 0.44278262818015435 1106 86-87 0.4067804336918499 1106 88-89 0.4209407038354982 1106 90-91 0.414905263593905 1106 92-93 0.45009547777716463 1106 94-95 0.4477490638566799 1106 96-97 0.4590859828832876 1106 98-99 0.449033377231423 1106 100 0.4829259891057056 1107 1 1.6609760157867406 1107 2 1.2640682388830697 1107 3 1.2064169836202687 1107 4 0.6521146468255736 1107 5 0.9774753392300966 1107 6 0.6576619545121076 1107 7 0.3802371735775054 1107 8 0.33278763491023966 1107 9 0.3405219201140781 1107 10-11 0.21040911476912072 1107 12-13 0.16959207919581587 1107 14-15 0.24853328365565375 1107 16-17 0.2442113427218402 1107 18-19 0.19333898677742667 1107 20-21 0.29261091798189653 1107 22-23 0.2782771029994535 1107 24-25 0.29905779819410583 1107 26-27 0.2742290712133766 1107 28-29 0.2831350458141628 1107 30-31 0.24771853063366223 1107 32-33 0.2906584546956168 1107 34-35 0.24146113482076004 1107 36-37 0.24119516361307802 1107 38-39 0.27064773887898497 1107 40-41 0.23753369735906915 1107 42-43 0.2699937994866417 1107 44-45 0.27522077281612667 1107 46-47 0.2473664046854509 1107 48-49 0.3679979057363383 1107 50-51 0.3433654436627549 1107 52-53 0.32562333535857846 1107 54-55 0.3215914313048742 1107 56-57 0.3440093968273956 1107 58-59 0.2639813020954591 1107 60-61 0.2725703947590361 1107 62-63 0.25453755175357884 1107 64-65 0.3058363599364995 1107 66-67 0.2932321264858544 1107 68-69 0.29844248688112884 1107 70-71 0.24734927658613515 1107 72-73 0.25835649940334804 1107 74-75 0.25395188386337963 1107 76-77 0.32330230983941277 1107 78-79 0.34354561939647965 1107 80-81 0.3212857674880958 1107 82-83 0.29466229851875525 1107 84-85 0.33141897835466594 1107 86-87 0.3267828628828511 1107 88-89 0.30084874323941335 1107 90-91 0.2812759479095952 1107 92-93 0.22384654109511715 1107 94-95 0.25514905004481747 1107 96-97 0.32520533444208155 1107 98-99 0.2615975145374563 1107 100 0.2390079860474401 1108 1 0.8601930590653915 1108 2 0.9679768136171987 1108 3 1.0463809925998824 1108 4 0.546855092954786 1108 5 0.896866925926652 1108 6 0.6084257224954825 1108 7 0.39458873987421583 1108 8 0.3294209184533514 1108 9 0.2872167047121934 1108 10-11 0.20641135456242665 1108 12-13 0.1305258144280188 1108 14-15 0.10541404315151937 1108 16-17 0.18003421252918628 1108 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-0.9049400818811009 2215 48-49 -0.9401612987732335 2215 50-51 -0.8985297145974087 2215 52-53 -0.7995923347586569 2215 54-55 -0.866949861160748 2215 56-57 -0.7659740554321885 2215 58-59 -0.8061776747985121 2215 60-61 -0.8262936666068228 2215 62-63 -0.8704969815280279 2215 64-65 -0.796285232399633 2215 66-67 -0.8424364856280775 2215 68-69 -0.828469060570427 2215 70-71 -0.8647650311822943 2215 72-73 -0.8740089717457309 2215 74-75 -0.8062211647998794 2215 76-77 -0.7171187876934333 2215 78-79 -0.7250442650886058 2215 80-81 -0.6902039874211674 2215 82-83 -0.7847468196576095 2215 84-85 -0.7597526316390457 2215 86-87 -0.7332264784784179 2215 88-89 -0.6937818201482742 2215 90-91 -0.7050050543982707 2215 92-93 -0.7010397162660134 2215 94-95 -0.7775416420846746 2215 96-97 -0.8324097754106745 2215 98-99 -0.8319473898053573 2215 100 -0.8401885569755976 2216 1 -6.29509502534772 2216 2 -6.396511773804608 2216 3 -5.679595904154784 2216 4 -3.05468005862555 2216 5 -3.9813743844494773 2216 6 -4.530924607494665 2216 7 -2.1012085533933202 2216 8 -1.2999107760790523 2216 9 -1.026708446509609 2216 10-11 -0.9360916340193128 2216 12-13 -0.7621902773028566 2216 14-15 -1.0943973504501585 2216 16-17 -0.9230583027678918 2216 18-19 -0.9111102140513125 2216 20-21 -0.9284499680739557 2216 22-23 -0.9489682977167604 2216 24-25 -1.4634741453268845 2216 26-27 -1.3175858389634172 2216 28-29 -2.0633401638600617 2216 30-31 -1.517418469833828 2216 32-33 -1.2309182833325139 2216 34-35 -1.2204102126484813 2216 36-37 -1.2688439402814353 2216 38-39 -1.2750539283754065 2216 40-41 -1.2956532431245833 2216 42-43 -1.271031024487506 2216 44-45 -1.3004416290315675 2216 46-47 -1.3713644549990462 2216 48-49 -1.3459653610091067 2216 50-51 -1.2547676212911725 2216 52-53 -1.2609465787797518 2216 54-55 -1.2022504590337348 2216 56-57 -1.1737897506574555 2216 58-59 -1.1250488912615424 2216 60-61 -1.14844526788508 2216 62-63 -1.2101836445671736 2216 64-65 -1.2357117523001122 2216 66-67 -1.2975270660250047 2216 68-69 -1.2693154637804867 2216 70-71 -1.2507227838696764 2216 72-73 -1.2978563584022282 2216 74-75 -1.3991108582366572 2216 76-77 -1.3541658206660223 2216 78-79 -1.3378575760323663 2216 80-81 -1.3247639248760592 2216 82-83 -1.3129120509236252 2216 84-85 -1.2446346979767071 2216 86-87 -1.1605340002454447 2216 88-89 -1.131402335078743 2216 90-91 -1.1612733267661035 2216 92-93 -1.1808360897050783 2216 94-95 -1.2138087566914955 2216 96-97 -1.318368903287702 2216 98-99 -1.3757921524513392 2216 100 -1.2503775228998855 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4320.0 3 3936.0 4 5015.0 5 6603.0 6 4896.0 7 3689.0 8 3510.0 9 3840.0 10 4008.0 11 7167.0 12 5298.0 13 7469.0 14 6779.0 15 7142.0 16 8191.0 17 10389.0 18 13623.0 19 22815.0 20 50942.0 21 73638.0 22 32937.0 23 20108.0 24 20933.0 25 22856.0 26 23641.0 27 25430.0 28 29098.0 29 34224.0 30 40462.0 31 50087.0 32 64750.0 33 88597.0 34 132732.0 35 298761.0 36 430661.0 37 821147.0 38 993498.0 39 308736.0 40 1567.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 14.55481905859923 40.00431802907166 26.449743044763117 18.991119867565995 2 19.188356827341043 36.7953929814444 25.111716680271666 18.904533510942887 3 20.44166839267415 25.10835942650525 20.000487343288672 34.44948483753193 4 31.295942419646284 26.281297702276607 22.35502975241249 20.067730125664617 5 18.57569203309022 35.838097074171394 23.779153769005323 21.80705712373307 6 34.91887764840618 22.97504125496312 23.84876654767368 18.25731454895702 7 20.552999259508947 23.241184839833274 36.250597333961466 19.955218566696313 8 33.498786379838066 23.401033438518258 23.518618544224374 19.581561637419302 9 33.31795494511296 25.3301006228518 23.191611197524296 18.160333234510944 10-11 18.405981326629657 39.498456069386854 23.233143675570158 18.862418928413334 12-13 18.362716072446286 24.99460538054065 30.14502524032116 26.497653306691898 14-15 18.736125082763852 31.92122735932311 22.988092158352647 26.354555399560386 16-17 26.19403877125754 24.609897404442176 30.09784665343706 19.098217170863226 18-19 19.013725482232953 24.8800932450159 30.120414404243135 25.985766868508016 20-21 26.42717805222425 32.21455559030132 22.791637730658902 18.566628626815522 22-23 25.922984317426224 25.131173201963612 30.14333715672245 18.802505323887715 24-25 18.776920847344435 32.24926797497286 22.79395091259278 26.17986026508992 26-27 19.217014400332715 28.244556011402427 33.77254925745577 18.765880330809086 28-29 26.31022271474624 24.881205129138866 30.141034938505268 18.667537217609624 30-31 24.199095929244752 26.212907444436475 30.40102401784856 19.186972608470214 32-33 21.015530352207147 27.05012386349037 31.379098362597624 20.55524742170486 34-35 20.827917083717885 26.973234519275348 31.631425838543763 20.567422558462997 36-37 20.98872436042129 26.98624941487808 31.70071059561857 20.32431562908206 38-39 21.538485077808843 26.73002459059789 31.415507715755382 20.315982615837882 40-41 20.519111346485413 27.700865975272706 31.387379894124884 20.392642784116997 42-43 20.664881349902004 27.043677082849626 31.75312808372947 20.5383134835189 44-45 20.568558227993254 27.423209967734262 31.215466434386208 20.792765369886276 46-47 21.04063739734382 26.995911012919464 31.53802365398817 20.425427935748548 48-49 20.625870270248317 27.196824414748544 31.407913646175956 20.76939166882718 50-51 21.070934410648963 27.600707259154163 30.854491989179913 20.47386634101697 52-53 21.155448795182355 27.46585686661313 30.790835707373816 20.587858630830706 54-55 21.175574954008553 28.25519796765733 29.377039965037294 21.192187113296825 56-57 21.550439293886033 28.72326901746467 28.487961775739105 21.238329912910192 58-59 22.310301881218507 28.92295099035122 27.553002477485798 21.21374465094447 60-61 22.02191230609916 29.406874188293337 27.31316006906166 21.258053436545847 62-63 21.505984364787686 29.36458191754177 27.166343843628383 21.963089874042158 64-65 21.58203027159519 29.27340481387521 27.85615508986787 21.288409824661734 66-67 21.64171860634908 28.7521493274387 28.337899927883047 21.268232138329164 68-69 21.8428857788138 28.726188830723707 28.188988572479868 21.241936817982634 70-71 20.942110461192502 29.477112989489463 28.342296885016303 21.23847966430173 72-73 20.961728227758872 28.84931522798184 28.557571468083033 21.63138507617626 74-75 21.15115617892343 29.235405610310846 27.913435503086752 21.700002707678976 76-77 21.725984742332244 28.85696105707072 27.98772075895728 21.42933344163976 78-79 21.573346024155438 29.134007093230828 27.463597942841204 21.829048939772527 80-81 22.09638140562038 29.57372762172229 27.05126879060501 21.278622182052324 82-83 21.927266671721167 29.60522111624985 27.16778407183164 21.29972814019734 84-85 21.82102558645613 29.87681828680106 26.61782612192049 21.684330004822318 86-87 21.831855360229966 29.9598206313483 26.780753490766273 21.427570517655457 88-89 22.569453800121398 29.67258011645104 26.510911175311175 21.247054908116382 90-91 22.087919928976678 30.202553757963145 26.41528414947555 21.294242163584627 92-93 21.752182456431093 30.21666220542068 26.292051982248708 21.73910335589952 94-95 21.85890030762491 30.47893663560453 26.152070379402247 21.51009267736831 96-97 21.939685978477208 29.996741268203102 26.415886473203326 21.64768628011636 98-99 22.486472756773914 30.01411079260357 25.77652685491527 21.722889595707244 100 21.740617402762126 30.75028825162729 25.77354063982384 21.73555370578675 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 97064.0 1 52075.5 2 5247.0 3 2885.5 4 2312.5 5 2168.5 6 2261.0 7 2622.5 8 2445.5 9 2196.0 10 2302.5 11 3183.0 12 26321.0 13 29662.5 14 9798.0 15 9166.0 16 10140.0 17 11237.5 18 12129.0 19 12911.5 20 13970.0 21 15423.0 22 16978.5 23 19186.0 24 21802.0 25 24277.0 26 27202.5 27 30910.5 28 35182.0 29 39567.0 30 44078.0 31 48660.0 32 53298.0 33 57945.0 34 62908.0 35 67821.5 36 72785.5 37 77748.0 38 81842.0 39 85321.5 40 88472.5 41 92453.0 42 97007.5 43 101164.0 44 106068.0 45 110247.0 46 113931.5 47 119781.5 48 123589.5 49 130862.5 50 156782.0 51 157201.5 52 129404.0 53 121408.5 54 121295.5 55 118695.5 56 110581.5 57 99104.5 58 87540.5 59 74133.5 60 62140.5 61 52688.5 62 43530.5 63 33470.5 64 24395.5 65 17686.5 66 13382.5 67 10370.0 68 8016.5 69 5916.0 70 4282.5 71 3209.5 72 2474.5 73 1801.5 74 1292.5 75 929.5 76 618.0 77 419.0 78 277.0 79 162.0 80 89.0 81 50.5 82 26.0 83 14.5 84 7.0 85 4.0 86 4.0 87 3.0 88 1.0 89 1.0 90 1.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.054040955788487596 2 0.0 3 0.0 4 3.5197015293103145E-4 5 0.008636806060384541 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.2183582216843395E-4 16-17 3.1135821220822015E-4 18-19 0.0 20-21 0.0 22-23 1.353731357427044E-5 24-25 0.003912283622964157 26-27 0.006687432905689597 28-29 0.0013131194167042327 30-31 0.005414925429708176 32-33 0.003100044808507931 34-35 0.024448388315132415 36-37 0.02405580622147857 38-39 0.022295955456823415 40-41 0.0023554925619230565 42-43 0.005942880659104723 44-45 0.006998791117897818 46-47 0.001015298518070283 48-49 0.00859619411966173 50-51 0.009882238909217422 52-53 0.001976447781843484 54-55 0.011290119520941546 56-57 0.006457298574927 58-59 0.011818074750338093 60-61 0.007404910525125931 62-63 0.0010017612044960127 64-65 0.012508477742625887 66-67 0.005103567217499956 68-69 0.002341955248348786 70-71 0.008054701576690911 72-73 0.005022343336054334 74-75 0.00798701500881956 76-77 0.007310149330106038 78-79 0.014552612092340723 80-81 0.007932865754522478 82-83 0.011235970266644465 84-85 0.006822806041432303 86-87 0.011655626987446849 88-89 0.018911627063255804 90-91 0.01688103002711524 92-93 0.015716821059727982 94-95 0.005374313488985365 96-97 0.00921891054407817 98-99 0.015622059864708087 100 0.014647373287360616 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3693495.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.68731483370385 #Duplication Level Percentage of deduplicated Percentage of total 1 78.56541677500402 45.32227932540815 2 13.940175327978885 16.083425659642973 3 3.7898059033113065 6.558711789088463 4 1.3459331625243791 3.1057308036666615 5 0.6243207066573366 1.8007692581071124 6 0.38026174487201775 1.3161767397387394 7 0.25657635773644466 1.0360840789339119 8 0.19584144900998174 0.903805386122206 9 0.14770940684920317 0.7668863151128671 >10 0.6993982185278221 7.658070155651685 >50 0.03252316466119722 1.284382356187724 >100 0.017663604699369978 1.9027131446383114 >500 0.0019754353069575442 0.8316459810307604 >1k 0.0022106061659661383 2.147448780857184 >5k 4.7034173743960385E-5 0.16621523806043403 >10k+ 1.4110252123188116E-4 9.11565498775281 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 218027 5.902999733314922 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 96844 2.622015191573293 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG 21094 0.5711121850713213 No Hit ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 6126 0.16585916591196145 No Hit TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 4289 0.11612307584009184 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC 3898 0.10553689662501234 No Hit CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 3760 0.10180059807851372 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 6.76865678713522E-4 0.0 0.0 2.7074627148540882E-5 2.7074627148540882E-5 2 7.039403058620629E-4 0.0 0.0 5.4149254297081764E-5 5.4149254297081764E-5 3 7.039403058620629E-4 0.0 0.0 5.4149254297081764E-5 5.4149254297081764E-5 4 7.039403058620629E-4 0.0 0.0 8.122388144562265E-5 5.4149254297081764E-5 5 7.039403058620629E-4 0.0 0.0 1.0829850859416353E-4 8.122388144562265E-5 6 7.039403058620629E-4 0.0 0.0 1.0829850859416353E-4 1.0829850859416353E-4 7 7.039403058620629E-4 0.0 0.0 1.624477628912453E-4 1.0829850859416353E-4 8 7.039403058620629E-4 0.0 0.0 1.624477628912453E-4 1.353731357427044E-4 9 7.039403058620629E-4 0.0 0.0 1.624477628912453E-4 1.353731357427044E-4 10-11 7.039403058620629E-4 0.0 0.0 1.624477628912453E-4 1.4891044931697483E-4 12-13 7.445522465848742E-4 0.0 0.0 2.0305970361405662E-4 2.707462714854088E-4 14-15 8.257761280304969E-4 0.0 0.0 2.4367164433686792E-4 2.9782089863394966E-4 16-17 8.799253823275786E-4 0.0 0.0 2.9782089863394966E-4 3.5197015293103145E-4 18-19 8.934626959018491E-4 0.0 0.0 3.248955257824906E-4 3.9258209365384275E-4 20-21 9.340746366246603E-4 0.0 0.0 3.248955257824906E-4 4.331940343766541E-4 22-23 9.476119501989308E-4 0.0 0.0 3.9258209365384275E-4 4.331940343766541E-4 24-25 0.0010017612044960125 0.0 0.0 4.4673134795092455E-4 4.60268661525195E-4 26-27 0.0010965223995159055 0.0 0.0 4.60268661525195E-4 4.738059750994654E-4 28-29 0.001204820908110069 0.0 0.0 4.8734328867373585E-4 5.685671701193585E-4 30-31 0.0013672686710013144 0.0 0.0 4.8734328867373585E-4 6.091791108421698E-4 32-33 0.0015297164338925597 0.0 0.0 5.144179158222767E-4 7.445522465848742E-4 34-35 0.0019222985275464026 0.0 0.0 5.144179158222767E-4 8.393134416047672E-4 36-37 0.002951134359190956 0.0 0.0 5.144179158222767E-4 9.2053732305039E-4 38-39 0.0041424179537267545 0.0 0.0 5.685671701193585E-4 9.476119501989308E-4 40-41 0.005956417972678993 0.0 0.0 5.685671701193585E-4 9.746865773474717E-4 42-43 0.008108850830987994 0.0 0.0 5.685671701193585E-4 0.001015298518070283 44-45 0.010532029960782403 0.0 0.0 6.091791108421698E-4 0.001110059713090176 46-47 0.013388403124953466 0.0 0.0 6.633283651392517E-4 0.0011642089673872579 48-49 0.016718582264223993 0.0 0.0 7.310149330106038E-4 0.0012454328488328806 50-51 0.02126711962517886 0.0 0.0 7.716268737334151E-4 0.0012725074759814214 52-53 0.027453671928620454 0.0 0.0 7.851641873076855E-4 0.0012860447895556917 54-55 0.03491273170804347 0.0 0.0 8.393134416047674E-4 0.001326656730278503 56-57 0.04402334374352747 0.0 0.0 8.663880687533082E-4 0.0013401940438527737 58-59 0.055543597595231614 0.0 0.0 9.2053732305039E-4 0.0013808059845755848 60-61 0.07092198581560284 0.0 0.0 9.88223890921742E-4 0.0014078806117241258 62-63 0.09045632930327507 0.0 0.0 0.0010829850859416352 0.001475567179595478 64-65 0.11597416539077486 0.0 0.0 0.0011100597130901761 0.0015838656881896414 66-67 0.1474213448238051 0.0 0.0 0.001137134340238717 0.001597403001763912 68-69 0.1855288825353764 0.0 0.0 0.0011777462809615283 0.0016244776289124528 70-71 0.23508898753078045 0.0 0.0 0.0012454328488328806 0.0016786268832095347 72-73 0.2975907643031871 0.0 0.0 0.0012454328488328806 0.0017057015103580755 74-75 0.37356216808199283 0.0 0.0 0.001258970162407151 0.0017057015103580755 76-77 0.46845873623762857 0.0 0.0 0.0013401940438527737 0.001719238823932346 78-79 0.5853805135786023 0.0 0.0 0.0013808059845755848 0.0017463134510808868 80-81 0.7307577240526926 0.0 0.0 0.0014349552388726666 0.0019222985275464026 82-83 0.911981740871451 0.0 0.0 0.0014349552388726666 0.002003522408992025 84-85 1.133844773040169 0.0 0.0 0.0014620298660212075 0.002125358231160459 86-87 1.39518261159146 0.0 0.0 0.0014620298660212075 0.0022201194261803523 88 1.6211474497731824 0.0 0.0 0.0014620298660212075 0.002274268680477434 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGAGT 69100 0.0 68.554825 1 ACGAGTG 72310 0.0 67.07902 2 TACGAGT 1565 0.0 66.9913 1 GTGGAAT 75135 0.0 66.302 6 TGGAATC 76060 0.0 65.711914 7 CGAGTGG 74260 0.0 65.653 3 GAATCAC 76230 0.0 65.62086 9 GGAATCA 76455 0.0 65.44003 8 AGTGGAA 75830 0.0 64.68328 5 GAGTGGA 76635 0.0 63.95563 4 AACGTGT 1310 0.0 46.655193 1 ACGTGTG 1620 0.0 42.35465 2 CGTGTGG 1785 0.0 42.125496 3 AGTACTT 31350 0.0 40.79005 20-21 GAGTACT 30990 0.0 40.103775 20-21 GTACTTT 31150 0.0 39.144493 22-23 TACTTTT 33190 0.0 37.956276 22-23 CATGGGG 17275 0.0 37.68066 24-25 ATCGAGT 800 0.0 36.43591 1 ACTTTTT 34115 0.0 35.57113 24-25 >>END_MODULE