FastQCFastQC Report
Thu 26 May 2016
SRR2132691_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132691_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1813422
Sequences flagged as poor quality0
Sequence length100
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1778059.804943361225352No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA204441.127371345445241No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG118190.651751219517575No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT46390.25581469729605133No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32470.179053744798508No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT28000.15440421479390898No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20630.11376281968565508No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC20470.11288050988683275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAGT510400.075.572041
ACGAGTG533150.073.885252
GTGGAAT556350.072.891076
CGAGTGG546500.072.630743
TGGAATC560900.072.584627
AGTGGAA555000.072.068775
GGAATCA564950.072.064288
GAATCAC568700.071.622139
GAGTGGA559000.071.511044
TACGAGT12050.064.386671
CGTGTGG9100.060.4232333
ACGTGTG8950.057.235172
AACGTGT8050.056.0756651
TCGAGTG5600.045.317432
ATCGAGT5550.042.3619651
AGTACTT243700.042.16745820-21
GAGTACT241750.041.38973620-21
GTACTTT240900.040.7565222-23
GTGTGGA14500.040.5136644
CGATTGG3600.040.4686473