##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132691_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1813422
Sequences flagged as poor quality	0
Sequence length	100
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.888774372429584	33.0	31.0	34.0	25.0	34.0
2	31.635697592728004	34.0	31.0	34.0	28.0	34.0
3	31.848044746341447	34.0	31.0	34.0	28.0	34.0
4	35.79852841754429	37.0	35.0	37.0	35.0	37.0
5	35.425225347437056	37.0	35.0	37.0	33.0	37.0
6	35.802930040553164	37.0	35.0	37.0	35.0	37.0
7	35.96958567834734	37.0	35.0	37.0	35.0	37.0
8	36.144717556090086	37.0	37.0	37.0	35.0	37.0
9	38.087540572464654	39.0	39.0	39.0	37.0	39.0
10-11	38.1690786810792	39.0	39.0	39.0	37.0	39.0
12-13	38.16701959058619	39.0	39.0	39.0	37.0	39.0
14-15	39.70953920267869	41.0	40.0	41.0	38.0	41.0
16-17	39.67018349838041	41.0	40.0	41.0	38.0	41.0
18-19	39.63549604008334	41.0	40.0	41.0	37.0	41.0
20-21	39.52038880084172	41.0	40.0	41.0	37.0	41.0
22-23	39.41977929020383	41.0	39.5	41.0	37.0	41.0
24-25	39.39727294584493	41.0	39.0	41.0	36.5	41.0
26-27	39.320902691155176	41.0	39.5	41.0	36.5	41.0
28-29	39.150824518506994	41.0	39.5	41.0	36.0	41.0
30-31	39.00640115759045	41.0	39.0	41.0	35.5	41.0
32-33	38.85602413558455	41.0	39.0	41.0	35.0	41.0
34-35	38.806711565206555	41.0	39.0	41.0	35.0	41.0
36-37	38.65080328792747	40.5	39.0	41.0	35.0	41.0
38-39	38.1208408743249	40.0	36.5	41.0	35.0	41.0
40-41	37.89013423240702	40.0	36.0	41.0	34.5	41.0
42-43	37.776546771793875	40.0	35.5	41.0	34.0	41.0
44-45	37.61191355349169	40.0	35.0	41.0	34.0	41.0
46-47	37.510847998976516	40.0	35.0	41.0	33.5	41.0
48-49	37.348126359997835	40.0	35.0	41.0	33.0	41.0
50-51	37.0473287519397	40.0	35.0	41.0	33.0	41.0
52-53	36.39689107113513	40.0	35.0	41.0	31.5	41.0
54-55	35.157895128657316	40.0	35.0	41.0	23.5	41.0
56-57	34.19770522250199	39.0	35.0	41.0	6.5	41.0
58-59	33.78833773936789	39.0	35.0	41.0	2.0	41.0
60-61	33.54364262703331	39.0	35.0	41.0	2.0	41.0
62-63	33.292768864610665	39.0	35.0	41.0	2.0	41.0
64-65	33.02786113767231	38.0	34.0	41.0	2.0	41.0
66-67	32.74133020333932	37.5	34.0	40.0	2.0	41.0
68-69	32.44907004547204	37.0	34.0	40.0	2.0	41.0
70-71	32.11961473942634	36.5	34.0	40.0	2.0	41.0
72-73	31.78010578894488	36.0	33.5	39.0	2.0	41.0
74-75	31.42138343970681	35.5	33.0	39.0	2.0	41.0
76-77	31.043585276896387	35.0	33.0	38.5	2.0	40.0
78-79	30.6849216564043	35.0	33.0	37.5	2.0	39.5
80-81	30.32864468391803	35.0	32.0	37.0	2.0	39.0
82-83	29.962520306911465	35.0	32.0	36.5	2.0	39.0
84-85	29.59033611591786	35.0	31.0	36.0	2.0	38.5
86-87	29.410701976704814	35.0	31.5	36.0	2.0	37.0
88-89	29.366192756015977	35.0	32.0	36.0	2.0	37.0
90-91	29.163347803214034	35.0	32.0	35.5	2.0	37.0
92-93	28.952014202981985	35.0	32.0	35.0	2.0	36.0
94-95	28.734524010406844	35.0	32.0	35.0	2.0	36.0
96-97	28.516244426283567	35.0	32.0	35.0	2.0	36.0
98-99	28.33904904649883	35.0	31.5	35.0	2.0	36.0
100	28.218985983405958	35.0	31.0	35.0	2.0	36.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.9134653704680744
1101	2	1.3788431240499328
1101	3	1.2694328049414132
1101	4	0.6718154819929936
1101	5	0.9759384085398253
1101	6	0.6565953127933284
1101	7	0.4785893599346309
1101	8	0.32476498287197586
1101	9	0.34135586514873495
1101	10-11	0.26133463350706165
1101	12-13	0.317870386022598
1101	14-15	0.3438345519761157
1101	16-17	0.39119626012855235
1101	18-19	0.3225649138767537
1101	20-21	0.43665993883629284
1101	22-23	0.4007896125541208
1101	24-25	0.413535717109319
1101	26-27	0.40136259018995446
1101	28-29	0.5251528104925427
1101	30-31	0.5359586659643867
1101	32-33	0.5502880788438986
1101	34-35	0.5662297512101375
1101	36-37	0.5480033033471372
1101	38-39	0.5344375593273156
1101	40-41	0.5541862075172048
1101	42-43	0.5622450471619302
1101	44-45	0.6490054625394208
1101	46-47	0.6493304734661578
1101	48-49	0.5768416964148315
1101	50-51	0.4870273547425086
1101	52-53	0.5514997880833263
1101	54-55	0.4503688655493079
1101	56-57	0.6321422394942005
1101	58-59	0.5670985571547504
1101	60-61	0.6395884682887782
1101	62-63	0.685589055262767
1101	64-65	0.6540096416611902
1101	66-67	0.6536786059742354
1101	68-69	0.7367246041571391
1101	70-71	0.6865663518074925
1101	72-73	0.6591766347476913
1101	74-75	0.621749675878231
1101	76-77	0.6268764000825762
1101	78-79	0.5156113404193157
1101	80-81	0.45823128503880994
1101	82-83	0.36814944482262035
1101	84-85	0.4120780634210526
1101	86-87	0.39979157810800103
1101	88-89	0.44347324559714707
1101	90-91	0.4712600041775197
1101	92-93	0.45622111134080257
1101	94-95	0.43132198803007427
1101	96-97	0.42134532578526773
1101	98-99	0.5271297001018738
1101	100	0.47347037689601734
1102	1	1.3625721629740468
1102	2	1.1619873702924615
1102	3	1.115094056033481
1102	4	0.5852076469786525
1102	5	0.8769999213185855
1102	6	0.5368640817037473
1102	7	0.33750208865413356
1102	8	0.3001111981906277
1102	9	0.2798454790309819
1102	10-11	0.20277916044069144
1102	12-13	0.18323983047843484
1102	14-15	0.2836772049924221
1102	16-17	0.30279120905589707
1102	18-19	0.3654718236905481
1102	20-21	0.41764390266500584
1102	22-23	0.4035165409895072
1102	24-25	0.3746318685102423
1102	26-27	0.3691859967928295
1102	28-29	0.4542287447509423
1102	30-31	0.42706302754594105
1102	32-33	0.4378397086433665
1102	34-35	0.40426001198078865
1102	36-37	0.4412070375095638
1102	38-39	0.47114961099744335
1102	40-41	0.4450111426765915
1102	42-43	0.44025259834054964
1102	44-45	0.5020632250225177
1102	46-47	0.36506852153502223
1102	48-49	0.36468594634885676
1102	50-51	0.45327999177595757
1102	52-53	0.4613920303171497
1102	54-55	0.3856936974832905
1102	56-57	0.3284918537319257
1102	58-59	0.31919792973561556
1102	60-61	0.24660451539784844
1102	62-63	0.2963982946896806
1102	64-65	0.30873770762701724
1102	66-67	0.31057748197932256
1102	68-69	0.30702333578336294
1102	70-71	0.29614081698361616
1102	72-73	0.2846220079930326
1102	74-75	0.18916766635961935
1102	76-77	0.18093490223390418
1102	78-79	0.18250257364176647
1102	80-81	0.14485754339767354
1102	82-83	0.15685966031080767
1102	84-85	0.18601610629446697
1102	86-87	0.14920145001277518
1102	88-89	0.13880342337729346
1102	90-91	0.15201779496215195
1102	92-93	0.1795865607678664
1102	94-95	0.14307406518526378
1102	96-97	0.16342756021224147
1102	98-99	0.1061578156979337
1102	100	0.07022369822512076
1103	1	1.8575376461020099
1103	2	1.3478177400228368
1103	3	1.225616109372428
1103	4	0.6129256169417943
1103	5	1.0032887687902914
1103	6	0.600472152675394
1103	7	0.3828742430540757
1103	8	0.33248843745753476
1103	9	0.3185624013186512
1103	10-11	0.252759578028261
1103	12-13	0.22883060594852367
1103	14-15	0.3246414305162091
1103	16-17	0.35904865906498173
1103	18-19	0.3615433687068119
1103	20-21	0.4396665723415296
1103	22-23	0.4476501160032669
1103	24-25	0.4257358147107695
1103	26-27	0.41798851751180877
1103	28-29	0.5245197649847881
1103	30-31	0.48533415309839256
1103	32-33	0.4736583026852017
1103	34-35	0.44907167312698704
1103	36-37	0.583475723952823
1103	38-39	0.5457050541536717
1103	40-41	0.5900020960612693
1103	42-43	0.6333123060017058
1103	44-45	0.5629019590999178
1103	46-47	0.511235321703225
1103	48-49	0.4396363178898781
1103	50-51	0.5441521438798063
1103	52-53	0.5672837615532913
1103	54-55	0.5332762043863397
1103	56-57	0.37897044198759744
1103	58-59	0.5102670048093998
1103	60-61	0.455736151851184
1103	62-63	0.33633829772052337
1103	64-65	0.3451764113959612
1103	66-67	0.4014080583582711
1103	68-69	0.41330704328181866
1103	70-71	0.2940933560385517
1103	72-73	0.36423148483100576
1103	74-75	0.3404020307709281
1103	76-77	0.33474269741170914
1103	78-79	0.29024356359995807
1103	80-81	0.33307298207979485
1103	82-83	0.2757791900442612
1103	84-85	0.39230320873377167
1103	86-87	0.2595869676871345
1103	88-89	0.2999837344260392
1103	90-91	0.27396644836788653
1103	92-93	0.35567049813825946
1103	94-95	0.267602381616058
1103	96-97	0.24600802031037006
1103	98-99	0.266028450410829
1103	100	0.21450819230021168
1104	1	1.8980986939601152
1104	2	1.396160366321478
1104	3	1.276479057897948
1104	4	0.6582288626087447
1104	5	0.9962392202803727
1104	6	0.611131870973594
1104	7	0.387748394072851
1104	8	0.323099703937757
1104	9	0.2675259558102354
1104	10-11	0.2688312084064677
1104	12-13	0.24318461619598963
1104	14-15	0.35890748256848326
1104	16-17	0.2646400370607864
1104	18-19	0.32976309865636466
1104	20-21	0.36955317853361436
1104	22-23	0.3745934811762197
1104	24-25	0.42413876702456577
1104	26-27	0.4514765390572677
1104	28-29	0.42009925286625815
1104	30-31	0.44895215412664413
1104	32-33	0.41917937461004584
1104	34-35	0.4386425148101907
1104	36-37	0.4631449018158875
1104	38-39	0.4857230087038289
1104	40-41	0.42616125487149503
1104	42-43	0.5003192283721489
1104	44-45	0.5496529972963131
1104	46-47	0.5563856831022207
1104	48-49	0.45334003279454294
1104	50-51	0.49329022996932537
1104	52-53	0.587915656665615
1104	54-55	0.48000021525457015
1104	56-57	0.3270007737170104
1104	58-59	0.23299168738154918
1104	60-61	0.28783826676348667
1104	62-63	0.35156727394507215
1104	64-65	0.3567293208130309
1104	66-67	0.2695821856604894
1104	68-69	0.3370380879405772
1104	70-71	0.29355869882243013
1104	72-73	0.30669447346774703
1104	74-75	0.2407192596580181
1104	76-77	0.29029499879347753
1104	78-79	0.26463970655510494
1104	80-81	0.29415967804463605
1104	82-83	0.29981329732269657
1104	84-85	0.34994789964650863
1104	86-87	0.27485303088141677
1104	88-89	0.24615693216644274
1104	90-91	0.36321226175835264
1104	92-93	0.3293494141163791
1104	94-95	0.36503924224386
1104	96-97	0.3965144245774397
1104	98-99	0.33509019782787064
1104	100	0.350604637522121
1105	1	1.6138169936010627
1105	2	1.2951975509164306
1105	3	1.1581157826040602
1105	4	0.5894628771677972
1105	5	0.915336903621089
1105	6	0.548679519884125
1105	7	0.27439962864075085
1105	8	0.21160279035751017
1105	9	0.17642000922619872
1105	10-11	0.15527509336047984
1105	12-13	0.13690172307710924
1105	14-15	0.16211901913825955
1105	16-17	0.139557804263589
1105	18-19	0.1875798255758525
1105	20-21	0.21751979970326119
1105	22-23	0.21018199133311555
1105	24-25	0.27878561174444627
1105	26-27	0.2381157845444548
1105	28-29	0.3083602520792894
1105	30-31	0.2532533136859385
1105	32-33	0.26499165019904325
1105	34-35	0.2670958311743661
1105	36-37	0.3511012554138375
1105	38-39	0.3563648241580637
1105	40-41	0.3312991907821754
1105	42-43	0.2895169129425099
1105	44-45	0.35290495858694726
1105	46-47	0.32332963568694595
1105	48-49	0.38809161289389493
1105	50-51	0.3742864610942078
1105	52-53	0.33263093136927324
1105	54-55	0.31996473987214813
1105	56-57	0.4881523095489868
1105	58-59	0.4443756269319934
1105	60-61	0.5441285377781924
1105	62-63	0.42383284130525567
1105	64-65	0.36092841948008925
1105	66-67	0.33598247954667215
1105	68-69	0.269087359988184
1105	70-71	0.31608756483984024
1105	72-73	0.2805017136428454
1105	74-75	0.21018629890842178
1105	76-77	0.24023920345630856
1105	78-79	0.27775207190850537
1105	80-81	0.2689281965631558
1105	82-83	0.2886473131113938
1105	84-85	0.23124431890773423
1105	86-87	0.27969200830505514
1105	88-89	0.32856787481559735
1105	90-91	0.3436380893123321
1105	92-93	0.2502986418966131
1105	94-95	0.20413348256651886
1105	96-97	0.213083948949091
1105	98-99	0.170663798486963
1105	100	0.2526246439162989
1106	1	1.5990431318501663
1106	2	1.2208809222148354
1106	3	1.0483325701965107
1106	4	0.5925958744399296
1106	5	0.9702069787075658
1106	6	0.5648946482623174
1106	7	0.31880382307817
1106	8	0.21945505701292944
1106	9	0.23464366568448014
1106	10-11	0.16272881464060163
1106	12-13	0.18949565147779168
1106	14-15	0.24445489050697233
1106	16-17	0.2820880884391812
1106	18-19	0.2446016941604583
1106	20-21	0.25526560596890846
1106	22-23	0.32473808121191183
1106	24-25	0.2983833225912136
1106	26-27	0.2925517220005176
1106	28-29	0.40915971564687226
1106	30-31	0.44529374297247415
1106	32-33	0.3991265562706374
1106	34-35	0.2466250115506412
1106	36-37	0.3847297847594291
1106	38-39	0.46024587396261296
1106	40-41	0.3764481861138762
1106	42-43	0.47575975933863646
1106	44-45	0.3942073539017841
1106	46-47	0.3564945868947973
1106	48-49	0.5171891163388693
1106	50-51	0.42882478121006784
1106	52-53	0.4706612120295759
1106	54-55	0.496738728173284
1106	56-57	0.5770859304325953
1106	58-59	0.591111070139057
1106	60-61	0.5977524445247298
1106	62-63	0.5546884263892053
1106	64-65	0.5808696615003868
1106	66-67	0.4696006388031151
1106	68-69	0.5545644895475093
1106	70-71	0.5706526011175796
1106	72-73	0.5817580763077181
1106	74-75	0.5732259829546571
1106	76-77	0.5532941789899262
1106	78-79	0.5713855402287642
1106	80-81	0.6209250538988513
1106	82-83	0.7096470072816459
1106	84-85	0.6398978946550855
1106	86-87	0.6348789862853152
1106	88-89	0.6188745658080315
1106	90-91	0.6202575808615904
1106	92-93	0.7186092313395136
1106	94-95	0.7144086590338006
1106	96-97	0.7176432728895819
1106	98-99	0.7528867413425004
1106	100	0.8353928952731984
1107	1	1.6181960007212979
1107	2	1.2564753082468236
1107	3	1.1804257298043588
1107	4	0.6105167299102447
1107	5	0.9532548823285367
1107	6	0.5864209978668313
1107	7	0.30169760275890667
1107	8	0.26145082649940576
1107	9	0.22624859194952052
1107	10-11	0.15187181007780737
1107	12-13	0.09510186439128177
1107	14-15	0.16120840035483042
1107	16-17	0.18082439788763338
1107	18-19	0.20854088409173954
1107	20-21	0.31028498813730465
1107	22-23	0.26406313293355765
1107	24-25	0.27918035994621704
1107	26-27	0.2965444822275387
1107	28-29	0.36505113470218475
1107	30-31	0.4142091240386776
1107	32-33	0.3921970572838376
1107	34-35	0.3713905070151
1107	36-37	0.4741378090973214
1107	38-39	0.43950830916558203
1107	40-41	0.4374983137027897
1107	42-43	0.4348317487726945
1107	44-45	0.4283235194770043
1107	46-47	0.4860008257704109
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2214	3	-3.52116243867685
2214	4	-1.9080673081984116
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2214	96-97	-0.4024848211895744
2214	98-99	-0.32777771793170274
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2215	2	-3.6099669829044068
2215	3	-3.52467488817031
2215	4	-1.7505729805973473
2215	5	-2.6448313542162722
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2215	7	-0.929070618724225
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2215	10-11	-0.4633239005908152
2215	12-13	-0.396299859065671
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2215	22-23	-0.7070207080146105
2215	24-25	-0.7257480962475142
2215	26-27	-0.686508146211402
2215	28-29	-0.8173616287747549
2215	30-31	-0.7483586635411399
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2215	100	-0.9107630544157956
2216	1	-6.047651721150402
2216	2	-6.135392770318443
2216	3	-5.545681112434494
2216	4	-3.031816712288162
2216	5	-3.8236018160748273
2216	6	-4.343259574508554
2216	7	-2.0829649976668208
2216	8	-1.308314563897035
2216	9	-1.037209295923752
2216	10-11	-0.8571780212581004
2216	12-13	-0.7111481154556216
2216	14-15	-0.95922237596983
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2216	18-19	-0.9036566640948749
2216	20-21	-0.9899258016657342
2216	22-23	-0.9507676525118143
2216	24-25	-1.428723002418316
2216	26-27	-1.335941872403275
2216	28-29	-1.975934883429609
2216	30-31	-1.606362631786027
2216	32-33	-1.3619076664622156
2216	34-35	-1.2735633550769663
2216	36-37	-1.3570768395041952
2216	38-39	-1.2441693118761776
2216	40-41	-1.1957646834278748
2216	42-43	-1.3134805163286458
2216	44-45	-1.3129536474638215
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2216	50-51	-1.2462661876642755
2216	52-53	-1.2400317526956925
2216	54-55	-1.137578157113282
2216	56-57	-1.1005173141318423
2216	58-59	-1.0617428912002396
2216	60-61	-1.1304482866474004
2216	62-63	-1.2344538480749847
2216	64-65	-1.1879016671250824
2216	66-67	-1.3779758924151935
2216	68-69	-1.4606205889912474
2216	70-71	-1.3250734060496896
2216	72-73	-1.306410553233956
2216	74-75	-1.2300522226231223
2216	76-77	-1.1883513669326042
2216	78-79	-1.2168911163360612
2216	80-81	-1.3722045272119345
2216	82-83	-1.3600983701500908
2216	84-85	-1.335597528154178
2216	86-87	-1.1550050087996553
2216	88-89	-1.1042472084675197
2216	90-91	-1.168245013022375
2216	92-93	-1.246027301798435
2216	94-95	-1.1004872625006143
2216	96-97	-1.2035112549323017
2216	98-99	-1.210143759958008
2216	100	-1.233988155261688
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1805.0
3	860.0
4	1022.0
5	1469.0
6	1139.0
7	987.0
8	1054.0
9	1168.0
10	1510.0
11	4046.0
12	2839.0
13	4359.0
14	4094.0
15	4475.0
16	5452.0
17	7177.0
18	10238.0
19	18786.0
20	46124.0
21	69315.0
22	28640.0
23	14559.0
24	12909.0
25	13124.0
26	13458.0
27	14363.0
28	16086.0
29	18645.0
30	20566.0
31	24036.0
32	28944.0
33	38235.0
34	54178.0
35	98898.0
36	156644.0
37	298687.0
38	483024.0
39	285328.0
40	5179.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.181436810878186	48.4047604202474	26.359980158478365	14.053822610396052
2	14.508206032572671	45.9274785460858	24.776582615629454	14.787732805712073
3	16.191156829463853	24.805864272077873	19.944833579828632	39.05814531862964
4	37.40697115278569	25.534405784442182	22.15796044592085	14.90066261685129
5	14.659055249688265	44.99572864280037	23.7989875587415	16.546228548769854
6	40.150654979693016	22.12681597979503	24.088143089143962	13.634385951368
7	16.363593250771196	22.236798715356933	46.188973112711764	15.210634921160104
8	39.35829608331651	22.75785779592395	23.212523064129584	14.671323056629952
9	38.631383097811764	24.880970893702624	22.968509260392782	13.519136748092833
10-11	13.829930374728	48.51013718814484	23.42827538212286	14.231657055004296
12-13	13.64541733804928	24.067288253919937	35.25823002037033	27.029064387660455
14-15	14.151268280026613	35.72944057928098	23.33998099172255	26.77931014896986
16-17	26.659755213230284	23.412070596085286	35.27697741553919	14.651196775145237
18-19	14.573579674229165	23.74174351033571	35.426640903220544	26.258035912214588
20-21	26.76194509606699	36.13767231234649	23.2606089481654	13.839773643421113
22-23	26.092271958760836	24.132303457220658	35.519311004278094	14.256113579740404
24-25	14.031597993319522	35.93603085571787	23.41515091187933	26.617220239083284
26-27	14.6009010750417	29.64277136689152	41.623446150163915	14.132881407902866
28-29	26.907645803296415	23.697890171107375	35.344479838490656	14.049984187105558
30-31	22.43451238612049	26.044272384355764	36.46132508327267	15.059890146251076
32-33	16.688366763062522	28.149216149504014	38.41873851576855	16.743678571664912
34-35	16.72698710641982	27.571180003491534	38.76096861673184	16.940864273356805
36-37	16.755605725599583	27.938457057865225	38.85781453441375	16.448122682121443
38-39	17.204422955152687	28.01682960138908	38.518093807156774	16.26065363630146
40-41	16.23749736333093	29.35954791292995	38.2582681116762	16.144686612062916
42-43	16.267599691834782	28.197283094534697	38.87925030041697	16.65586691321355
44-45	16.198156548603677	28.952973983384283	38.14679282086888	16.70207664714315
46-47	16.904126487645133	28.337849265562458	38.379541793029595	16.378482453762818
48-49	16.467736006858456	28.470775875072007	38.276336263912626	16.78515185415692
50-51	16.903290172499943	29.187168889786	37.514242633814575	16.395298303899473
52-53	17.2835220465678	29.028732296501968	37.01093677405835	16.676808882871878
54-55	17.987810006405773	30.050101794691564	34.59285018794033	17.369238010962334
56-57	18.90723340893267	31.080840738162042	32.510536801251874	17.50138905165342
58-59	19.848344337441297	31.504370896209515	31.127217194701153	17.520067571648035
60-61	18.993872392899235	32.337011221170165	31.154864501626623	17.514251884303984
62-63	18.097822040978386	32.27556599637696	31.291252043818364	18.335359918826292
64-65	18.27471414736164	32.12626821945187	31.98232158890607	17.61669604428041
66-67	18.07480499942647	31.525929120379764	32.698745069838445	17.700520810355325
68-69	18.508056121949572	31.6877254948997	32.32077248331493	17.483445899835804
70-71	17.365706692447112	32.63006060077965	32.1907414847453	17.813491222027945
72-73	17.380213734614923	31.856698487611286	32.53434922119523	18.22873855657857
74-75	17.606624550876738	32.281973577902725	31.76878045283206	18.34262141838848
76-77	18.234320087907605	31.832231041476227	31.978734522071345	17.954714348544822
78-79	18.003206073062394	32.152368167273956	31.481012166035207	18.363413593628447
80-81	18.38708138575863	32.64058453850648	31.130734459132846	17.841599616602036
82-83	18.383977264485935	32.43230126210672	31.27363054844795	17.910090924959402
84-85	18.22711769719534	32.82262105036468	30.623572859085268	18.32668839335471
86-87	18.183743478308827	32.937298006816754	30.870514786176773	18.00844372869765
88-89	19.099260391786345	32.57480676946005	30.324543470700966	18.001389368052642
90-91	18.552674723143078	33.1394362783017	30.21217263153134	18.09571636702388
92-93	18.181206471246032	33.26241418346228	29.951891721211904	18.604487624079784
94-95	18.518547113799638	33.453519299884576	29.782479577301284	18.2454540090145
96-97	18.48956990489668	32.9335605878653	29.967348476220863	18.609521031017156
98-99	19.043779281581088	33.06915506618061	29.260939718654143	18.626125933584156
100	18.31905585534705	33.72632247759653	29.170288432264524	18.784333234791895
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	18976.0
1	10279.0
2	1261.5
3	795.5
4	669.5
5	664.5
6	712.5
7	892.0
8	946.5
9	1032.5
10	1319.5
11	2011.5
12	11143.5
13	14412.5
14	9811.0
15	11898.5
16	14980.0
17	17580.0
18	19041.5
19	20640.0
20	22882.0
21	25366.5
22	28011.0
23	30770.5
24	33115.0
25	35371.5
26	38619.5
27	41806.0
28	44237.0
29	46581.0
30	48344.0
31	49669.0
32	51043.0
33	52162.5
34	53684.0
35	54081.5
36	53805.5
37	53433.5
38	52654.0
39	52216.5
40	51237.5
41	50231.5
42	48756.5
43	46908.5
44	45654.5
45	44427.0
46	43052.5
47	42781.0
48	41727.0
49	42436.5
50	54152.0
51	52576.5
52	36246.5
53	29736.5
54	27453.5
55	26469.0
56	23595.5
57	19235.0
58	16485.5
59	13311.5
60	10064.0
61	7939.0
62	6025.0
63	4259.5
64	3007.5
65	2162.5
66	1602.5
67	1174.0
68	830.0
69	617.0
70	451.0
71	345.5
72	300.0
73	208.0
74	146.5
75	124.5
76	92.0
77	66.0
78	40.0
79	25.0
80	15.5
81	6.5
82	3.5
83	3.5
84	2.5
85	1.0
86	0.0
87	0.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05850816853440622
2	0.0
3	0.0
4	3.308661745583764E-4
5	0.009981129599177688
6	3.860105369847724E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	8.27165436395941E-5
16-17	3.860105369847724E-4
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0043564046316852895
26-27	0.007913216008187835
28-29	0.0015716143291522877
30-31	0.005624724967492398
32-33	0.00363952792014214
34-35	0.02566970070948737
36-37	0.026276288696177725
38-39	0.023491498393644724
40-41	0.0030053677522385853
42-43	0.006010735504477171
44-45	0.007637494196055855
46-47	0.0015164699667258917
48-49	0.010229279230096469
50-51	0.011249449934984796
52-53	0.0024539241279746245
54-55	0.01177332137803556
56-57	0.00741691674635027
58-59	0.012986497351416272
60-61	0.008437087451238598
62-63	0.0014888977855126936
64-65	0.013786090606599015
66-67	0.00518357006808123
68-69	0.002729645940106605
70-71	0.008905814531862963
72-73	0.005431719699000012
74-75	0.009209108525208141
76-77	0.008519803994878191
78-79	0.01657088090913202
80-81	0.00896095889428936
82-83	0.01232476500229952
84-85	0.007582349833629459
86-87	0.013344935707187847
88-89	0.020265553191700553
90-91	0.019107521580746238
92-93	0.01789434560736552
94-95	0.0066724678535939235
96-97	0.010118990505243678
98-99	0.017398046345527957
100	0.01709475235218278
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1813422.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.3199016893858
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.24917673824424	61.428856072559924
2	5.941794467971194	8.000020388847144
3	0.9032637560812178	1.824228817769188
4	0.3764672947651743	1.0137496509144226
5	0.2442091394400212	0.8220067629375869
6	0.17617633565691468	0.711610415785184
7	0.15404590543547556	0.7259248646698047
8	0.12077824108812606	0.6504623453015665
9	0.1132965652428785	0.6864402270506099
>10	0.6877324722857737	7.096185435757084
>50	0.018060082380291772	0.8254895914383439
>100	0.01040937499072741	1.6990783095811122
>500	0.003442219813520844	1.6890902717714797
>1k	9.015337606840305E-4	1.2366100562231064
>5k	0.0	0.0
>10k+	2.458728438229174E-4	11.590246789393454
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	177805	9.804943361225352	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20444	1.127371345445241	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	11819	0.651751219517575	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4639	0.25581469729605133	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3247	0.179053744798508	No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2800	0.15440421479390898	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2063	0.11376281968565508	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	2047	0.11288050988683275	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.271654363959409E-4	0.0	0.0	0.0	0.0
2	8.271654363959409E-4	0.0	0.0	0.0	0.0
3	8.271654363959409E-4	0.0	0.0	0.0	0.0
4	8.271654363959409E-4	0.0	0.0	0.0	0.0
5	8.271654363959409E-4	0.0	0.0	0.0	0.0
6	8.271654363959409E-4	0.0	0.0	0.0	0.0
7	8.271654363959409E-4	0.0	0.0	0.0	0.0
8	8.271654363959409E-4	0.0	0.0	0.0	0.0
9	8.271654363959409E-4	0.0	0.0	0.0	0.0
10-11	8.271654363959409E-4	0.0	0.0	0.0	0.0
12-13	8.271654363959409E-4	0.0	0.0	0.0	0.0
14-15	8.271654363959409E-4	0.0	0.0	0.0	0.0
16-17	9.37454161248733E-4	0.0	0.0	0.0	0.0
18-19	9.925985236751292E-4	0.0	0.0	0.0	0.0
20-21	9.925985236751292E-4	0.0	0.0	0.0	0.0
22-23	0.0010201707048883273	0.0	0.0	0.0	0.0
24-25	0.0011856037921675153	0.0	0.0	0.0	0.0
26-27	0.0013234646982335056	0.0	0.0	0.0	0.0
28-29	0.0014613256042994957	0.0	0.0	0.0	0.0
30-31	0.0015991865103654858	0.0	0.0	0.0	0.0
32-33	0.0019024805037106641	0.0	0.0	0.0	0.0
34-35	0.0022609188594822386	0.0	0.0	0.0	0.0
36-37	0.002839934664959397	0.0	0.0	0.0	0.0
38-39	0.004328832450472091	2.757218121319803E-5	0.0	0.0	0.0
40-41	0.005928018960837576	5.514436242639606E-5	0.0	0.0	0.0
42-43	0.0076099220148426565	5.514436242639606E-5	0.0	0.0	0.0
44-45	0.009181536343994944	5.514436242639606E-5	0.0	0.0	0.0
46-47	0.011332166478624391	5.514436242639606E-5	0.0	0.0	0.0
48-49	0.01442025077450257	5.514436242639606E-5	0.0	0.0	0.0
50-51	0.01852850577526908	5.514436242639606E-5	0.0	0.0	0.0
52-53	0.02354664275607112	8.27165436395941E-5	0.0	0.0	0.0
54-55	0.031432286583045754	1.1028872485279213E-4	0.0	0.0	0.0
56-57	0.038325331886345265	1.1028872485279213E-4	0.0	0.0	0.0
58-59	0.047672301317619396	1.1028872485279213E-4	0.0	0.0	0.0
60-61	0.06071394303146206	1.1028872485279213E-4	0.0	0.0	0.0
62-63	0.07797412847092403	1.1028872485279213E-4	0.0	0.0	0.0
64-65	0.09892898619295454	1.1028872485279213E-4	0.0	0.0	0.0
66-67	0.1260324403255282	1.1028872485279213E-4	0.0	0.0	0.0
68-69	0.16005651194261455	1.1028872485279213E-4	0.0	0.0	0.0
70-71	0.20367570262189386	1.1028872485279213E-4	0.0	0.0	0.0
72-73	0.2589027815919295	1.1028872485279213E-4	0.0	0.0	0.0
74-75	0.32948756549771646	1.1028872485279213E-4	0.0	0.0	0.0
76-77	0.4175806844738842	1.1028872485279213E-4	0.0	0.0	0.0
78-79	0.5224928339901027	1.1028872485279213E-4	0.0	0.0	0.0
80-81	0.6527438180412501	1.1028872485279213E-4	0.0	0.0	0.0
82-83	0.8124694638093064	1.1028872485279213E-4	0.0	2.757218121319803E-5	0.0
84-85	1.011788761799515	1.1028872485279213E-4	0.0	5.514436242639606E-5	0.0
86-87	1.244994270500744	1.3786090606599015E-4	0.0	5.514436242639606E-5	0.0
88	1.4445617181218713	1.6543308727918818E-4	0.0	5.514436242639606E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGAGT	51040	0.0	75.57204	1
ACGAGTG	53315	0.0	73.88525	2
GTGGAAT	55635	0.0	72.89107	6
CGAGTGG	54650	0.0	72.63074	3
TGGAATC	56090	0.0	72.58462	7
AGTGGAA	55500	0.0	72.06877	5
GGAATCA	56495	0.0	72.06428	8
GAATCAC	56870	0.0	71.62213	9
GAGTGGA	55900	0.0	71.51104	4
TACGAGT	1205	0.0	64.38667	1
CGTGTGG	910	0.0	60.423233	3
ACGTGTG	895	0.0	57.23517	2
AACGTGT	805	0.0	56.075665	1
TCGAGTG	560	0.0	45.31743	2
ATCGAGT	555	0.0	42.361965	1
AGTACTT	24370	0.0	42.167458	20-21
GAGTACT	24175	0.0	41.389736	20-21
GTACTTT	24090	0.0	40.75652	22-23
GTGTGGA	1450	0.0	40.513664	4
CGATTGG	360	0.0	40.468647	3
>>END_MODULE