FastQCFastQC Report
Thu 26 May 2016
SRR2132690_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132690_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4011697
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2739166.827933415709113No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1322403.29636061746438No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG263480.6567794128021135No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT110140.2745471554805859No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT71500.17822881438952146No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57150.14245841597707903No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA56290.14031468478302325No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC54540.1359524410741888No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT50240.1252337851039099No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41140.1025501178179708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT30550.083.336651
AACGAGT854350.067.492671
GTGGAAT951300.066.840476
ACGAGTG907600.066.716092
TGGAATC967100.066.1420447
GAATCAC974350.065.7849359
CGAGTGG935600.065.611473
GGAATCA976350.065.587618
AGTGGAA958850.064.780185
GAGTGGA965450.064.351944
CGTGTGG22150.062.585743
AACGTGT19050.061.0171551
ATCGAGT12500.056.0952721
ACGTGTG23000.055.6227762
TCGAGTG15900.050.879512
CGATTGG6200.045.476463
AGTACTT418000.040.7490920-21
GACGAGT11100.040.700361
GAGTACT409100.040.53280620-21
GTGTGGA38650.040.2444154