##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132690_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4011697
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.128464338159137	31.0	28.0	34.0	16.0	34.0
2	30.258555170043998	33.0	30.0	34.0	19.0	34.0
3	30.718255640941976	33.0	31.0	34.0	25.0	34.0
4	35.166287733096496	37.0	35.0	37.0	32.0	37.0
5	34.621272493909686	37.0	35.0	37.0	30.0	37.0
6	35.17495638379469	37.0	35.0	37.0	32.0	37.0
7	35.56678482946244	37.0	35.0	37.0	33.0	37.0
8	35.77050510045998	37.0	35.0	37.0	35.0	37.0
9	37.68307701204752	39.0	38.0	39.0	35.0	39.0
10-11	37.77048839929836	39.0	39.0	39.0	35.0	39.0
12-13	37.76112041861586	39.0	39.0	39.0	35.0	39.0
14-15	39.17140539278016	41.0	39.0	41.0	36.0	41.0
16-17	39.10180529586357	41.0	39.0	41.0	36.0	41.0
18-19	38.96792878425265	41.0	39.0	41.0	35.5	41.0
20-21	38.80686377859544	40.5	39.0	41.0	35.0	41.0
22-23	38.70209813951553	40.5	39.0	41.0	35.0	41.0
24-25	38.631208189451996	40.5	39.0	41.0	35.0	41.0
26-27	38.57179929092352	40.5	39.0	41.0	35.0	41.0
28-29	38.40042792364429	41.0	38.0	41.0	35.0	41.0
30-31	38.27088611128906	40.5	38.0	41.0	34.5	41.0
32-33	38.13079402557072	40.0	38.0	41.0	34.0	41.0
34-35	38.04871217841227	40.0	38.0	41.0	34.0	41.0
36-37	37.89038790815957	40.0	38.0	41.0	34.0	41.0
38-39	37.460875659353135	40.0	36.0	41.0	33.5	41.0
40-41	37.263542585594074	40.0	35.0	41.0	33.0	41.0
42-43	37.121358990970656	40.0	35.0	41.0	33.0	41.0
44-45	36.984126418321225	40.0	35.0	41.0	33.0	41.0
46-47	36.84285490653955	40.0	35.0	41.0	33.0	41.0
48-49	36.684858677013736	40.0	35.0	41.0	32.5	41.0
50-51	36.42375595664379	39.0	35.0	41.0	31.5	41.0
52-53	35.93100550714573	39.0	35.0	41.0	30.5	41.0
54-55	35.08749875676054	39.0	35.0	41.0	25.5	41.0
56-57	34.40559294483108	39.0	35.0	41.0	20.0	41.0
58-59	34.07706788423951	38.5	35.0	41.0	16.0	41.0
60-61	33.82032267142807	38.0	35.0	40.0	12.0	41.0
62-63	33.51812562115235	37.5	34.0	40.0	8.5	41.0
64-65	33.24403812152313	37.0	34.0	40.0	7.0	41.0
66-67	32.92221047601551	36.0	34.0	40.0	6.0	41.0
68-69	32.62771378297015	36.0	34.0	39.0	2.0	41.0
70-71	32.29649721801023	35.0	33.0	39.0	2.0	41.0
72-73	31.960586380277476	35.0	33.0	38.5	2.0	40.0
74-75	31.621105233022334	35.0	33.0	37.5	2.0	40.0
76-77	31.260547842970194	35.0	33.0	37.0	2.0	39.0
78-79	31.138211834044295	35.0	33.0	37.0	2.0	39.0
80-81	30.997228479618474	35.0	33.0	36.0	2.0	39.0
82-83	30.686699045316733	35.0	33.0	36.0	2.0	38.0
84-85	30.404841766464415	35.0	33.0	36.0	2.0	37.0
86-87	30.21068640029394	35.0	33.0	35.0	2.0	37.0
88-89	30.003682481503464	35.0	33.0	35.0	2.0	36.0
90-91	29.805616426165784	35.0	33.0	35.0	2.0	36.0
92-93	29.64419084990716	35.0	33.0	35.0	2.0	36.0
94-95	29.4615236394972	35.0	32.5	35.0	2.0	36.0
96-97	29.276622337130647	35.0	32.0	35.0	2.0	35.5
98-99	29.128337459184976	35.0	32.0	35.0	2.0	35.0
100	29.017790476199973	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	2.791212526830577
1101	2	2.2168299985323863
1101	3	2.040382169672178
1101	4	1.026343647275695
1101	5	1.5807981351631213
1101	6	0.957805734358331
1101	7	0.5353465671296931
1101	8	0.40006219559793266
1101	9	0.34718119981335605
1101	10-11	0.29624396154702026
1101	12-13	0.26958781759971373
1101	14-15	0.35602689540317556
1101	16-17	0.37708796109777865
1101	18-19	0.4378126341898749
1101	20-21	0.4869412879344779
1101	22-23	0.4507317268329274
1101	24-25	0.49367542059622593
1101	26-27	0.46582972442209325
1101	28-29	0.504129491675144
1101	30-31	0.47335565488373277
1101	32-33	0.4914989130616263
1101	34-35	0.5750957732590223
1101	36-37	0.5360882010764172
1101	38-39	0.4958537896033306
1101	40-41	0.5635514040524967
1101	42-43	0.5527783937197981
1101	44-45	0.5519173966057949
1101	46-47	0.5719431527390313
1101	48-49	0.5248105786117492
1101	50-51	0.5643568814421798
1101	52-53	0.4944489057615016
1101	54-55	0.4742126611379689
1101	56-57	0.44901183103804243
1101	58-59	0.44106932301149726
1101	60-61	0.40721492321469555
1101	62-63	0.40310027602532017
1101	64-65	0.35021645524295764
1101	66-67	0.33633837834785396
1101	68-69	0.3219674517140305
1101	70-71	0.4413107357898838
1101	72-73	0.4759191989771345
1101	74-75	0.4822771419071401
1101	76-77	0.4099832084372004
1101	78-79	0.5110203796876576
1101	80-81	0.5590749310125531
1101	82-83	0.6064877814939109
1101	84-85	0.6502357500095997
1101	86-87	0.6883162581211231
1101	88-89	0.615634515026148
1101	90-91	0.5663659336364155
1101	92-93	0.6290484717366809
1101	94-95	0.6112347371413449
1101	96-97	0.6187014932007848
1101	98-99	0.6566822226840259
1101	100	0.6256549204378352
1102	1	2.809166760554767
1102	2	2.305601377064253
1102	3	2.0955146196401557
1102	4	1.1103923863731282
1102	5	1.6039167925969764
1102	6	1.0433189502997493
1102	7	0.5771456435045934
1102	8	0.48512026748431936
1102	9	0.5134345441379864
1102	10-11	0.4172930096285299
1102	12-13	0.4023615252340136
1102	14-15	0.5168425957024638
1102	16-17	0.5261943698639158
1102	18-19	0.5777016209713537
1102	20-21	0.5797308837367652
1102	22-23	0.546476112826987
1102	24-25	0.5907142809483261
1102	26-27	0.5262367379475776
1102	28-29	0.5974197658779232
1102	30-31	0.6122877794826707
1102	32-33	0.5871480572139021
1102	34-35	0.5889954989676482
1102	36-37	0.5685112899777138
1102	38-39	0.4676829847329955
1102	40-41	0.46822326492939226
1102	42-43	0.5777451160465219
1102	44-45	0.5758619248629984
1102	46-47	0.535505905775473
1102	48-49	0.5889695395957517
1102	50-51	0.5555528007900179
1102	52-53	0.5181456941359528
1102	54-55	0.5798985463938635
1102	56-57	0.49005414282800785
1102	58-59	0.35416861275261624
1102	60-61	0.35581440952119436
1102	62-63	0.4334585383184688
1102	64-65	0.4512230420132113
1102	66-67	0.44227881156192694
1102	68-69	0.4685397751668887
1102	70-71	0.4144900662791926
1102	72-73	0.4217510317090465
1102	74-75	0.3798898831376931
1102	76-77	0.2898788449137726
1102	78-79	0.4900563641833635
1102	80-81	0.4963215527472542
1102	82-83	0.5327738148354229
1102	84-85	0.5198251994183245
1102	86-87	0.5529782418763709
1102	88-89	0.5873506362838405
1102	90-91	0.6119842263824644
1102	92-93	0.5667243440512522
1102	94-95	0.5806308286622723
1102	96-97	0.6016820310210811
1102	98-99	0.6383295883619127
1102	100	0.6955985236513484
1103	1	2.5804879710794886
1103	2	2.1480084174946192
1103	3	1.97869513515937
1103	4	0.9917560890215071
1103	5	1.5553365982108502
1103	6	0.9970724568405416
1103	7	0.5206731629430124
1103	8	0.4037970059414917
1103	9	0.388260155578422
1103	10-11	0.29062269102111316
1103	12-13	0.2671042203167886
1103	14-15	0.42057212495866025
1103	16-17	0.44655353929550046
1103	18-19	0.41370793273071627
1103	20-21	0.4142834724427331
1103	22-23	0.38566813879253203
1103	24-25	0.5034800999527036
1103	26-27	0.4661800793456976
1103	28-29	0.4709833431931827
1103	30-31	0.5268971105858924
1103	32-33	0.4949698793604398
1103	34-35	0.5059945726633117
1103	36-37	0.5002737432765585
1103	38-39	0.5098425138997698
1103	40-41	0.4586241543300318
1103	42-43	0.5464887277043786
1103	44-45	0.5142466443450786
1103	46-47	0.5534966020154144
1103	48-49	0.5117904369001423
1103	50-51	0.5671081535620388
1103	52-53	0.5524162551987857
1103	54-55	0.6250476720567093
1103	56-57	0.5821432670162849
1103	58-59	0.5151805919468515
1103	60-61	0.520022282090153
1103	62-63	0.5467145405270699
1103	64-65	0.5428778222041899
1103	66-67	0.5985814912196119
1103	68-69	0.6139979988749857
1103	70-71	0.6086396960077209
1103	72-73	0.594622698319565
1103	74-75	0.6175758167583822
1103	76-77	0.635370665501938
1103	78-79	0.6394626009093223
1103	80-81	0.5973179555640584
1103	82-83	0.6149399509012135
1103	84-85	0.6434766493902693
1103	86-87	0.6673698681211455
1103	88-89	0.6297995547134398
1103	90-91	0.6361402369312295
1103	92-93	0.6189253277413549
1103	94-95	0.6946665354983956
1103	96-97	0.613798531548909
1103	98-99	0.6049388786359735
1103	100	0.666542986838838
1104	1	1.5730046405997626
1104	2	1.724658871875068
1104	3	1.814606385481767
1104	4	0.8868247047824411
1104	5	1.4691287843472196
1104	6	0.928110212477435
1104	7	0.4412485421918362
1104	8	0.2916821937037639
1104	9	0.2852784932549355
1104	10-11	0.22509295891136816
1104	12-13	0.15093445723044852
1104	14-15	0.2543450306332815
1104	16-17	0.22089186542036998
1104	18-19	0.23691563909143554
1104	20-21	0.3202743625011877
1104	22-23	0.2501941308517672
1104	24-25	0.2873559708686315
1104	26-27	0.23812925717829359
1104	28-29	0.3400046156335961
1104	30-31	0.30700813526163984
1104	32-33	0.24667766497991295
1104	34-35	0.2066578776926491
1104	36-37	0.2678956550856313
1104	38-39	0.299086113754214
1104	40-41	0.22965829843823116
1104	42-43	0.22093566242624973
1104	44-45	0.2220369711845933
1104	46-47	0.35305303754278583
1104	48-49	0.29698781641258165
1104	50-51	0.32388964983621804
1104	52-53	0.3368325417371878
1104	54-55	0.32588046933511805
1104	56-57	0.19637428289912862
1104	58-59	0.21077188315976514
1104	60-61	0.18442434474486902
1104	62-63	0.0886133364944186
1104	64-65	0.2223757244741833
1104	66-67	0.2533674892604765
1104	68-69	0.15770428339470044
1104	70-71	0.06601834798684081
1104	72-73	0.06598795031208837
1104	74-75	0.19917575681072464
1104	76-77	0.13700491234001433
1104	78-79	0.10698098368888509
1104	80-81	0.05012600943724976
1104	82-83	0.11278866101949347
1104	84-85	0.044431816076713204
1104	86-87	0.052260930009108364
1104	88-89	0.07769457756930365
1104	90-91	-0.005392523651675418
1104	92-93	-0.05721100106254795
1104	94-95	-0.013212218509973894
1104	96-97	0.03009432045609728
1104	98-99	0.07132100924303941
1104	100	0.06934611988102901
1105	1	2.1837110795840147
1105	2	1.9769174576822444
1105	3	1.9542011973086986
1105	4	1.013486620996332
1105	5	1.583418952182413
1105	6	1.0550459159473888
1105	7	0.5371111947259095
1105	8	0.3636515532833471
1105	9	0.3136997735867837
1105	10-11	0.22870653714750944
1105	12-13	0.20306844968909843
1105	14-15	0.3020197476208111
1105	16-17	0.3369406020968313
1105	18-19	0.32136495769836415
1105	20-21	0.3505399425017828
1105	22-23	0.33108930490072197
1105	24-25	0.360335172792567
1105	26-27	0.2538983745696086
1105	28-29	0.2993047933514248
1105	30-31	0.366942572651098
1105	32-33	0.3352725489119308
1105	34-35	0.2913665625382862
1105	36-37	0.3193407788731051
1105	38-39	0.3202277493520711
1105	40-41	0.24456448759956828
1105	42-43	0.32874268164437837
1105	44-45	0.4024759215041911
1105	46-47	0.39741155675879014
1105	48-49	0.36957510598237775
1105	50-51	0.32675996247539274
1105	52-53	0.37298141965479203
1105	54-55	0.3274561775555185
1105	56-57	0.2733143869001182
1105	58-59	0.23684905893075836
1105	60-61	0.2999465552323386
1105	62-63	0.30657487267207983
1105	64-65	0.30531969033879136
1105	66-67	0.2916946830577416
1105	68-69	0.26256490850338565
1105	70-71	0.2530569284497588
1105	72-73	0.3222438088503985
1105	74-75	0.3211064659430498
1105	76-77	0.27496426415769903
1105	78-79	0.2780521577717714
1105	80-81	0.3500734681570883
1105	82-83	0.3534725247493604
1105	84-85	0.34033020304350714
1105	86-87	0.321737097157186
1105	88-89	0.29352005356891553
1105	90-91	0.3433125008004332
1105	92-93	0.33758391298394486
1105	94-95	0.3740779344800558
1105	96-97	0.3804631167677073
1105	98-99	0.35320354581516256
1105	100	0.350685111342937
1106	1	1.6423428170069272
1106	2	1.0174607706232166
1106	3	1.5358869451776798
1106	4	0.7570068791555897
1106	5	1.4973752242985583
1106	6	0.9959774858506094
1106	7	0.5040191144275212
1106	8	0.401775394451235
1106	9	0.3559980326777392
1106	10-11	0.33147678219518895
1106	12-13	0.2865350583563142
1106	14-15	0.41693231653372465
1106	16-17	0.37766068995983915
1106	18-19	0.32707960040014683
1106	20-21	0.40811249580419684
1106	22-23	0.35741218599259383
1106	24-25	0.4255874087022491
1106	26-27	0.41800419580172843
1106	28-29	0.4556604379628766
1106	30-31	0.425611099619303
1106	32-33	0.4484239243935946
1106	34-35	0.4336596541249662
1106	36-37	0.3706247879925897
1106	38-39	0.39499305210611624
1106	40-41	0.48101683395501027
1106	42-43	0.5060275669257734
1106	44-45	0.5231621352198204
1106	46-47	0.5583420623103876
1106	48-49	0.5030926184229472
1106	50-51	0.47051283545351197
1106	52-53	0.5186724152400473
1106	54-55	0.5802647546870787
1106	56-57	0.518780480359446
1106	58-59	0.5107627261311478
1106	60-61	0.5609842672157015
1106	62-63	0.539480151547636
1106	64-65	0.49056515450407545
1106	66-67	0.4886161616735336
1106	68-69	0.5045181788394402
1106	70-71	0.5388887115318113
1106	72-73	0.4260774028727461
1106	74-75	0.48338761828107835
1106	76-77	0.2836480622579103
1106	78-79	0.2910133382457012
1106	80-81	0.3664492120463194
1106	82-83	0.4395785711921114
1106	84-85	0.37206608341655567
1106	86-87	0.3867710638568589
1106	88-89	0.3042998531005985
1106	90-91	0.40873086811588877
1106	92-93	0.37849465861624765
1106	94-95	0.439581372798564
1106	96-97	0.43666373625120514
1106	98-99	0.3894946968883062
1106	100	0.3977839047692555
1107	1	1.7079801608463328
1107	2	1.163003135298105
1107	3	1.4742594742994157
1107	4	0.7694721407914713
1107	5	1.4754474704484721
1107	6	1.0592600977052768
1107	7	0.5814222796359161
1107	8	0.43777048992784984
1107	9	0.46139448567143404
1107	10-11	0.2352943241570884
1107	12-13	0.16088272478842924
1107	14-15	0.24767983125965287
1107	16-17	0.26741443271896514
1107	18-19	0.26030875632648787
1107	20-21	0.24290636291899403
1107	22-23	0.27319980397037824
1107	24-25	0.30332796339455825
1107	26-27	0.22460036974288045
1107	28-29	0.26132465297081353
1107	30-31	0.177087159768746
1107	32-33	0.137593649604014
1107	34-35	0.08998440303496125
1107	36-37	0.18133899187581193
1107	38-39	0.2010638921399135
1107	40-41	0.25383614803323695
1107	42-43	0.15143938951637637
1107	44-45	0.15048581446858122
1107	46-47	0.19943574570151412
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2216	4	-2.946083497308109
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2216	90-91	-1.0613807537634905
2216	92-93	-1.069210431853417
2216	94-95	-1.0908421364725456
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2216	98-99	-1.117967988105292
2216	100	-1.2011706298261053
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7769.0
3	6462.0
4	7045.0
5	9181.0
6	7741.0
7	5630.0
8	5278.0
9	5374.0
10	5943.0
11	10653.0
12	7595.0
13	10065.0
14	9671.0
15	10048.0
16	11910.0
17	14731.0
18	20031.0
19	33151.0
20	70960.0
21	95899.0
22	42499.0
23	26346.0
24	25525.0
25	28071.0
26	29290.0
27	32367.0
28	36965.0
29	43294.0
30	51336.0
31	63174.0
32	81153.0
33	111521.0
34	161574.0
35	351876.0
36	450643.0
37	785541.0
38	975791.0
39	357325.0
40	2269.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	13.973823415556893	42.295606739644896	26.890561583864443	16.840008260933764
2	17.543442156365323	40.101234904190534	24.992903279557904	17.362419659886243
3	18.887966862901163	25.68646136535237	20.479936545556658	34.945635226189815
4	32.490938373461404	26.903751704079347	22.37130570927964	18.23400421317961
5	17.26025170894013	39.05928089783451	23.853222214937965	19.82724517828739
6	35.81726137342875	23.790655176599827	23.939046244020922	16.453037205950498
7	18.951356495767254	23.94684842848301	38.91624915839855	18.185545917351188
8	34.662238947757025	24.314174275873775	23.379906308975976	17.643680467393224
9	34.325473733435004	26.24216135964406	23.10715390519274	16.325211001728196
10-11	16.698095593959366	42.93275389442423	23.249338122993834	17.11981238862257
12-13	16.63142804653492	25.731467256874087	31.508635871552613	26.128468825038382
14-15	17.00183239162878	33.958534256201304	23.0566765137048	25.98295683846512
16-17	25.898778446516996	25.31452415998724	31.406799370340966	17.3798980231548
18-19	17.35561035641525	25.635634994367717	31.46923857908511	25.539516070131917
20-21	26.019071568172773	34.29797527394082	22.918062850749383	16.764890307137026
22-23	25.568881052902633	25.808530617138757	31.537374139733092	17.085214190225518
24-25	17.093800952564465	34.25084197535357	22.865099233566244	25.790257838515718
26-27	17.53858656059089	29.52245333685645	35.95614963864146	16.982810463911196
28-29	25.677160705478798	25.600035496540897	31.801834577893406	16.920969220086903
30-31	23.15906677646901	27.109682644719165	32.18925423609241	17.541996342719422
32-33	19.48352582719848	28.31945664158052	33.22666049795557	18.970357033265422
34-35	19.4597552228128	28.102873972590785	33.45494293746335	18.982427867133065
36-37	19.50801950002973	28.163585161559794	33.69091198948192	18.637483348928555
38-39	20.12200898488724	27.837818297857485	33.393839219381825	18.646333497873442
40-41	18.954750101394293	29.148958499259763	33.24320645772587	18.653084941620072
42-43	19.09659224336375	28.20004746498585	33.703006797165465	19.000353494484937
44-45	19.04933845428071	28.849749883307958	32.97346276542376	19.12744889698757
46-47	19.736404558577846	28.071889983104008	33.39396580219074	18.7977396561274
48-49	19.083887450753252	28.449359906041526	33.30169975043372	19.165052892771502
50-51	19.59430975644475	29.02660667262153	32.56924772523133	18.809835845702388
52-53	19.748613692196667	28.576175572814034	32.620081240317035	19.055129494672265
54-55	19.914177019587676	29.518528568004598	30.874436435368718	19.692857977039004
56-57	20.14097611407711	30.16204587986681	29.84548215239404	19.851495853662033
58-59	21.19376562087725	30.37901905159674	28.677727677966608	19.749487649559402
60-61	20.782961069574892	30.808474443810564	28.489510773663874	19.91905371295067
62-63	20.08811389695849	30.886079609614654	28.317096359431314	20.70871013399554
64-65	20.458382563244363	30.766318850775377	28.922782030208914	19.852516555771345
66-67	20.575764151941765	30.15843772799099	29.345015114326284	19.920783005740965
68-69	20.782218224534855	30.278758917513127	29.192564933057575	19.746457924894443
70-71	19.55222174592809	31.20424870431446	29.295512781600248	19.948016768157203
72-73	19.600125887679525	30.383352912399005	29.606015187538365	20.41050601238311
74-75	19.913102220804117	30.729707025358895	28.92432215494527	20.43286859889172
76-77	20.550927893645902	30.247076845223926	29.150817461277	20.051177799853175
78-79	20.3783426187924	30.704866753984426	28.47361413385945	20.443176493363723
80-81	20.675665501800648	31.209268537598717	28.233263547737486	19.881802412863152
82-83	20.730663350814186	31.103180730496327	28.290099034336073	19.876056884353414
84-85	20.450837637949103	31.59119115011797	27.69955591297345	20.258415298959477
86-87	20.470653813127765	31.713378763042364	27.86130572666864	19.954661697161228
88-89	21.56652052924564	31.12880376296173	27.49432197911964	19.81035372867299
90-91	20.859560446463785	31.81991776506855	27.378339017917284	19.94218277055038
92-93	20.393107866894027	31.79397144025289	27.330401571583852	20.48251912126923
94-95	20.665764724124745	32.05323804352758	27.19606057025305	20.08493666209463
96-97	20.596358412867026	31.377049846487832	27.63717653282503	20.389415207820115
98-99	21.26123817508975	31.543231257410532	26.79423487678437	20.40129569071535
100	20.55497279877475	32.310678275713535	26.721913338970342	20.412435586541374
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	135783.0
1	73289.5
2	7897.0
3	4173.0
4	3203.5
5	2890.0
6	2915.5
7	3341.0
8	3127.5
9	2774.5
10	2811.5
11	4012.5
12	31372.0
13	35290.5
14	12134.0
15	11672.0
16	12895.5
17	14228.0
18	15467.5
19	16700.5
20	18213.0
21	20511.0
22	23433.0
23	26821.0
24	30517.0
25	34751.0
26	41735.0
27	49363.0
28	54046.0
29	57837.5
30	62748.5
31	68146.0
32	73976.5
33	80798.0
34	87863.5
35	93875.5
36	99809.5
37	105247.0
38	108914.5
39	110418.5
40	111530.0
41	114283.0
42	115265.5
43	115161.0
44	116962.5
45	117850.5
46	118059.0
47	121219.5
48	122065.5
49	128783.5
50	161749.5
51	160675.5
52	121669.5
53	106349.5
54	102964.0
55	100184.0
56	91603.5
57	79557.0
58	71309.0
59	59673.5
60	46671.5
61	37965.0
62	29986.0
63	22140.5
64	15395.0
65	11112.0
66	8469.0
67	6356.0
68	4801.0
69	3733.5
70	2909.0
71	2113.0
72	1725.5
73	1350.5
74	962.5
75	698.0
76	479.5
77	323.0
78	194.5
79	122.5
80	73.5
81	38.0
82	17.5
83	11.5
84	8.0
85	3.5
86	4.0
87	3.0
88	0.5
89	0.5
90	1.0
91	0.5
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06144531852729655
2	0.06987068066207393
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	4.985421381525075E-5
18-19	0.0
20-21	4.6115147779106943E-4
22-23	1.4956264144575226E-4
24-25	0.0
26-27	0.002480247137308725
28-29	9.596936159435769E-4
30-31	0.006144531852729656
32-33	0.0013460637730117703
34-35	0.0022309760682324713
36-37	0.007939283550078682
38-39	0.01288731427124232
40-41	0.0032405238979912993
42-43	0.007951747103532495
44-45	0.0017324339300799638
46-47	0.002368075156224411
48-49	0.003427477199798489
50-51	0.010220113832126405
52-53	0.0035396491808828037
54-55	0.001682579716264713
56-57	6.107141192368217E-4
58-59	9.596936159435769E-4
60-61	0.006767709525420289
62-63	0.0017698245904414019
64-65	0.008263335939877811
66-67	0.010880682165178476
68-69	0.01218935527782881
70-71	0.01005808763722684
72-73	0.004599051224456883
74-75	0.002168658300963408
76-77	0.004786004526264072
78-79	0.016863187823008566
80-81	0.00904853980746801
82-83	0.006481047795982599
84-85	0.0051973017902398905
86-87	0.0074407414119261744
88-89	0.004387170815742066
90-91	0.006555829116705474
92-93	0.011391687856784797
94-95	0.008849122952207009
96-97	0.011254588768792857
98-99	0.002280830282047722
100	0.0028167630805616675
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4011697.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.78146782763235
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.09651986714314	46.04779433769136
2	13.073097071059586	14.84619281495769
3	3.134312769053482	5.339126389732426
4	1.0095159290211468	2.2928718498078653
5	0.42333091099352094	1.2018675251510451
6	0.24555998410271127	0.8365953802249205
7	0.16329273494369612	0.6490400822984105
8	0.12220682622289786	0.5551266377194025
9	0.09256124256019976	0.47301868948658904
>10	0.5284372906449012	6.637626829127355
>50	0.08084711829766371	3.0158281916419076
>100	0.02513270247974187	2.614045974407087
>500	0.001994443654367687	0.8096185170820018
>1k	0.002792221095140796	2.7905175205409227
>5k	2.2160484882069808E-4	0.7291121677168176
>10k+	1.7728387905655845E-4	11.161617092414316
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	273916	6.827933415709113	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	132240	3.29636061746438	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	26348	0.6567794128021135	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11014	0.2745471554805859	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7150	0.17822881438952146	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5715	0.14245841597707903	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA	5629	0.14031468478302325	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	5454	0.1359524410741888	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5024	0.1252337851039099	No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4114	0.1025501178179708	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0010469384901202658	0.0	0.0	2.4927106907625375E-5	0.0
2	0.0010469384901202658	4.985421381525075E-5	0.0	2.4927106907625375E-5	0.0
3	0.0010469384901202658	7.478132072287613E-5	0.0	1.4956264144575226E-4	0.0
4	0.0010469384901202658	7.478132072287613E-5	0.0	1.7448974835337764E-4	0.0
5	0.0010469384901202658	1.2463553453812687E-4	0.0	2.2434396216862838E-4	0.0
6	0.0010469384901202658	1.2463553453812687E-4	0.0	2.2434396216862838E-4	0.0
7	0.0010469384901202658	1.2463553453812687E-4	0.0	2.7419817598387913E-4	0.0
8	0.0010469384901202658	1.2463553453812687E-4	0.0	2.991252828915045E-4	0.0
9	0.0010469384901202658	1.2463553453812687E-4	0.0	2.991252828915045E-4	0.0
10-11	0.0010469384901202658	1.3709908799193956E-4	0.0	3.240523897991299E-4	0.0
12-13	0.001071865597027891	2.118804087148157E-4	0.0	3.9883371052200605E-4	0.0
14-15	0.0011466469177507672	2.2434396216862838E-4	0.0	4.860785846986948E-4	0.0
16-17	0.0012089646850198307	2.3680751562244108E-4	0.0	5.110056916063202E-4	0.0
18-19	0.0012837460057427068	2.6173462253006646E-4	0.0	5.483963519677583E-4	0.0
20-21	0.0013336002195579576	2.991252828915045E-4	0.0	5.733234588753837E-4	0.0
22-23	0.0014956264144575227	3.240523897991299E-4	0.0	5.98250565783009E-4	0.0
24-25	0.001557944181726586	3.240523897991299E-4	0.0	6.107141192368217E-4	0.0
26-27	0.0016451890559032747	3.489794967067553E-4	0.0	6.605683330520725E-4	0.0
28-29	0.0017448974835337764	4.1129726397581866E-4	0.0	6.730318865058851E-4	0.0
30-31	0.001956777892248592	4.237608174296314E-4	0.0	6.979589934135106E-4	0.0
32-33	0.002355611602770598	4.7361503124488215E-4	0.0	6.979589934135106E-4	0.0
34-35	0.00264227333220829	4.7361503124488215E-4	0.0	7.478132072287613E-4	0.0
36-37	0.003277914558352737	4.7361503124488215E-4	0.0	7.727403141363867E-4	0.0
38-39	0.00398833710522006	4.7361503124488215E-4	0.0	8.225945279516374E-4	0.0
40-41	0.00560859905421571	4.860785846986948E-4	0.0	8.350580814054502E-4	0.0
42-43	0.007540449839556676	5.359327985139455E-4	0.0	8.475216348592628E-4	0.0
44-45	0.009821280121604398	5.483963519677583E-4	0.0	8.475216348592628E-4	0.0
46-47	0.012662970309073691	5.60859905421571E-4	0.0	8.599851883130755E-4	0.0
48-49	0.015841176439795927	5.733234588753837E-4	0.0	8.724487417668882E-4	0.0
50-51	0.01980458643810836	6.231776726906345E-4	0.0	8.724487417668882E-4	0.0
52-53	0.02522623219051688	6.854954399596978E-4	0.0	8.724487417668882E-4	0.0
54-55	0.03178206130722236	6.979589934135106E-4	0.0	8.724487417668882E-4	0.0
56-57	0.039160484951879466	7.228861003211359E-4	0.0	8.973758486745135E-4	0.0
58-59	0.0479348265833636	7.852038675901994E-4	0.0	8.973758486745135E-4	0.0
60-61	0.060884458621874984	9.22302955582139E-4	0.0	8.973758486745135E-4	0.0
62-63	0.07631433779769509	9.347665090359517E-4	0.0	8.973758486745135E-4	0.0
64-65	0.09530879326130562	9.596936159435769E-4	0.0	9.22302955582139E-4	0.0
66-67	0.11890229994937304	9.721571693973896E-4	0.0	9.22302955582139E-4	0.0
68-69	0.14840353097454767	9.97084276305015E-4	0.0	9.22302955582139E-4	0.0
70-71	0.18841153756128642	9.97084276305015E-4	0.0	9.22302955582139E-4	0.0
72-73	0.23637129125155765	0.0010469384901202658	0.0	9.22302955582139E-4	0.0
74-75	0.29300567814568246	0.0011092562573893293	0.0	9.22302955582139E-4	0.0
76-77	0.36454647497056736	0.0011715740246583926	0.0	9.22302955582139E-4	0.0
78-79	0.4531124858133603	0.001196501131566018	0.0	9.22302955582139E-4	0.0
80-81	0.5630036366156268	0.0012214282384736435	0.0	9.472300624897643E-4	0.0
82-83	0.7011995173115018	0.001246355345381269	0.0	9.846207228512024E-4	0.0
84-85	0.8680366438442384	0.0012837460057427068	0.0	9.97084276305015E-4	0.0
86-87	1.065621356747531	0.0013460637730117703	0.0	9.97084276305015E-4	0.0
88	1.2317480607334004	0.0014208450937346464	0.0	0.0010220113832126404	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	3055	0.0	83.33665	1
AACGAGT	85435	0.0	67.49267	1
GTGGAAT	95130	0.0	66.84047	6
ACGAGTG	90760	0.0	66.71609	2
TGGAATC	96710	0.0	66.142044	7
GAATCAC	97435	0.0	65.784935	9
CGAGTGG	93560	0.0	65.61147	3
GGAATCA	97635	0.0	65.58761	8
AGTGGAA	95885	0.0	64.78018	5
GAGTGGA	96545	0.0	64.35194	4
CGTGTGG	2215	0.0	62.58574	3
AACGTGT	1905	0.0	61.017155	1
ATCGAGT	1250	0.0	56.095272	1
ACGTGTG	2300	0.0	55.622776	2
TCGAGTG	1590	0.0	50.87951	2
CGATTGG	620	0.0	45.47646	3
AGTACTT	41800	0.0	40.74909	20-21
GACGAGT	1110	0.0	40.70036	1
GAGTACT	40910	0.0	40.532806	20-21
GTGTGGA	3865	0.0	40.244415	4
>>END_MODULE