FastQCFastQC Report
Thu 26 May 2016
SRR2132689_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132689_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2376831
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1499806.310082626825382No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA314101.3215075030576426No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG116970.4921258600211795No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT59730.2513009969997867No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41520.17468637862767694No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA30110.12668128276684373No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT26380.1109881182128641No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT17500.080.395561
ATCGAGT7000.073.9424061
AACGAGT459050.071.373231
ACGAGTG489350.070.217292
GTGGAAT518750.069.71246
TGGAATC525600.069.215177
CGAGTGG505850.069.1396943
GGAATCA530550.068.684558
GAATCAC531050.068.628739
AGTGGAA518750.068.145125
GAGTGGA521950.067.790374
TCGAGTG8650.067.410912
CGTGTGG11850.063.057733
AACGTGT10650.057.4371221
ACGTGTG13850.049.2314722
CGATTGG4750.045.5117763
GACGAGT5550.044.086871
ACGATTG4400.042.7495842
AGTACTT226250.041.03482420-21
GAGTACT220650.040.8515720-21
Produced by FastQC (version 0.11.5)