##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132689_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2376831
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.170757618021643	31.0	28.0	34.0	16.0	34.0
2	30.300807672064188	33.0	30.0	34.0	19.0	34.0
3	30.770117437882625	33.0	31.0	34.0	25.0	34.0
4	35.21893395028927	37.0	35.0	37.0	32.0	37.0
5	34.685719767202635	37.0	35.0	37.0	32.0	37.0
6	35.257791572055396	37.0	35.0	37.0	32.0	37.0
7	35.65544710583125	37.0	35.0	37.0	35.0	37.0
8	35.87092982210346	37.0	35.0	37.0	35.0	37.0
9	37.8018079535314	39.0	39.0	39.0	35.0	39.0
10-11	37.90695362859202	39.0	39.0	39.0	35.0	39.0
12-13	37.917386217194235	39.0	39.0	39.0	35.0	39.0
14-15	39.36300856055816	41.0	39.0	41.0	37.0	41.0
16-17	39.32512387292155	41.0	39.0	41.0	36.5	41.0
18-19	39.29384440879474	41.0	39.5	41.0	36.5	41.0
20-21	39.18122260270083	41.0	39.0	41.0	36.0	41.0
22-23	39.09584779060859	40.5	39.0	41.0	36.0	41.0
24-25	39.04455281002309	41.0	39.0	41.0	35.5	41.0
26-27	38.996445266827976	41.0	39.0	41.0	35.5	41.0
28-29	38.84892194691166	41.0	39.0	41.0	35.0	41.0
30-31	38.732287655285546	41.0	39.0	41.0	35.0	41.0
32-33	38.60082521643314	40.5	39.0	41.0	35.0	41.0
34-35	38.53789583693582	40.0	38.0	41.0	35.0	41.0
36-37	38.40072832271205	40.0	38.0	41.0	35.0	41.0
38-39	38.01024052614595	40.0	37.0	41.0	34.5	41.0
40-41	37.82543416002231	40.0	37.0	41.0	34.0	41.0
42-43	37.70078268080482	40.0	36.5	41.0	33.0	41.0
44-45	37.579973712897555	40.0	36.0	41.0	33.0	41.0
46-47	37.44947600397336	40.0	36.0	41.0	33.0	41.0
48-49	37.31050714165205	40.0	35.5	41.0	33.0	41.0
50-51	37.07079468418243	40.0	35.0	41.0	33.0	41.0
52-53	36.610025281561875	40.0	35.0	41.0	31.5	41.0
54-55	35.815385906696775	39.5	35.0	41.0	28.5	41.0
56-57	35.153498082110175	39.0	35.0	41.0	24.0	41.0
58-59	34.82261717387564	39.0	35.0	41.0	22.5	41.0
60-61	34.56905749714641	39.0	35.0	41.0	21.5	41.0
62-63	34.28233896309834	38.5	35.0	40.5	20.0	41.0
64-65	34.01261238178061	38.0	35.0	40.0	19.0	41.0
66-67	33.69995300465199	37.0	34.0	40.0	18.0	41.0
68-69	33.3874703333977	37.0	34.0	40.0	16.0	41.0
70-71	33.034398533172954	36.0	34.0	39.0	12.0	41.0
72-73	32.67644333989249	36.0	34.0	39.0	9.0	41.0
74-75	32.306521162001005	35.0	33.5	38.5	7.0	40.0
76-77	31.925860526053388	35.0	33.0	37.5	2.0	39.5
78-79	31.79368179731752	35.0	33.5	37.0	2.0	39.0
80-81	31.635655206449258	35.0	34.0	37.0	2.0	39.0
82-83	31.3114720819444	35.0	33.5	36.0	2.0	39.0
84-85	31.02547888343765	35.0	33.0	36.0	2.0	37.0
86-87	30.81617603439201	35.0	33.0	36.0	2.0	37.0
88-89	30.596315009354893	35.0	33.0	35.0	2.0	36.5
90-91	30.39422659835722	35.0	33.0	35.0	2.0	36.0
92-93	30.229761813103245	35.0	33.0	35.0	2.0	36.0
94-95	30.05813139428087	35.0	33.0	35.0	2.0	36.0
96-97	29.88152712582426	35.0	33.0	35.0	2.0	36.0
98-99	29.739765048503656	35.0	33.0	35.0	2.0	35.5
100	29.636572814810982	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	2.88075575759715
1101	2	2.2930170007041752
1101	3	2.0555642215674474
1101	4	1.0123418786545173
1101	5	1.6373637394971823
1101	6	1.0647490917245719
1101	7	0.5969478348784634
1101	8	0.4649533596219513
1101	9	0.41755117068117187
1101	10-11	0.34245552997965234
1101	12-13	0.306729089649906
1101	14-15	0.4151801489689717
1101	16-17	0.35295389064251736
1101	18-19	0.3783353951085502
1101	20-21	0.4281948048116817
1101	22-23	0.4031079419813892
1101	24-25	0.44150487888300205
1101	26-27	0.37322569568527086
1101	28-29	0.3694469103803968
1101	30-31	0.44560095734797045
1101	32-33	0.45426999624039155
1101	34-35	0.5021688914842173
1101	36-37	0.4741336418926778
1101	38-39	0.4267230414294758
1101	40-41	0.44753962632348276
1101	42-43	0.44347236849139193
1101	44-45	0.4349075533884914
1101	46-47	0.4814976071601933
1101	48-49	0.40095828513525333
1101	50-51	0.44277645145150757
1101	52-53	0.5084605631287786
1101	54-55	0.5105726748283317
1101	56-57	0.32475116550668304
1101	58-59	0.21721608609150422
1101	60-61	0.25844166258563916
1101	62-63	0.20409253370839053
1101	64-65	0.2020258395712986
1101	66-67	0.19756689177064857
1101	68-69	0.2525845130154778
1101	70-71	0.291000717475967
1101	72-73	0.206360313157397
1101	74-75	0.27185944474305046
1101	76-77	0.2645263525038075
1101	78-79	0.2982128371412145
1101	80-81	0.3038086551440742
1101	82-83	0.34112826082073155
1101	84-85	0.33121638476669446
1101	86-87	0.3096996052722929
1101	88-89	0.22229187510979997
1101	90-91	0.1781299586179017
1101	92-93	0.2426110170734077
1101	94-95	0.275134766707712
1101	96-97	0.2642801549992413
1101	98-99	0.21592001849343134
1101	100	0.15968637757105242
1102	1	2.871322799633063
1102	2	2.2511778274795375
1102	3	2.04439374154283
1102	4	1.0183041889025404
1102	5	1.5474426549668792
1102	6	1.0096128345725646
1102	7	0.497338800894255
1102	8	0.40849456963821495
1102	9	0.41101826987035395
1102	10-11	0.27948401554374414
1102	12-13	0.21427858060901883
1102	14-15	0.3046112698638339
1102	16-17	0.3220722073218525
1102	18-19	0.3500968147705308
1102	20-21	0.3603463426113436
1102	22-23	0.32655451938009605
1102	24-25	0.3359079412931152
1102	26-27	0.2541471036653675
1102	28-29	0.4261607703611716
1102	30-31	0.4245641594381553
1102	32-33	0.4177569891621786
1102	34-35	0.3573262670816604
1102	36-37	0.42113976859707236
1102	38-39	0.3307858691971788
1102	40-41	0.3268972487476631
1102	42-43	0.39133249686641136
1102	44-45	0.37331313431060664
1102	46-47	0.38376269782034456
1102	48-49	0.39858587508884114
1102	50-51	0.3612042970246421
1102	52-53	0.18058311605835797
1102	54-55	0.30341658360920576
1102	56-57	0.17201055654178532
1102	58-59	0.17417528117255898
1102	60-61	0.12453234785955658
1102	62-63	0.11242411371354422
1102	64-65	0.12403385044926551
1102	66-67	0.15054961703929592
1102	68-69	0.18182563070723745
1102	70-71	0.1370120790425915
1102	72-73	0.08454338888233792
1102	74-75	0.09286868661440195
1102	76-77	0.006188648934553953
1102	78-79	0.011546112902578898
1102	80-81	0.09210016505796759
1102	82-83	0.112243094919247
1102	84-85	0.07190071660734887
1102	86-87	0.04909885223092303
1102	88-89	0.09858732294645378
1102	90-91	0.07803433680922467
1102	92-93	0.18120407326574295
1102	94-95	0.1932207132851076
1102	96-97	0.17721359342759158
1102	98-99	0.1637353977461693
1102	100	0.16344137848173546
1103	1	2.5667784031409937
1103	2	2.2064224265459345
1103	3	1.9939958276809584
1103	4	0.9771819145338512
1103	5	1.544967410556545
1103	6	0.9334234967767259
1103	7	0.5164506194077916
1103	8	0.3988845566784889
1103	9	0.3924245257682841
1103	10-11	0.24865386971539039
1103	12-13	0.15857457190209345
1103	14-15	0.34858578450328537
1103	16-17	0.36471772111829637
1103	18-19	0.3086238100018548
1103	20-21	0.4125601906428571
1103	22-23	0.28762360225941563
1103	24-25	0.39317293619729554
1103	26-27	0.429690814424454
1103	28-29	0.4174033625576641
1103	30-31	0.492223670994953
1103	32-33	0.3454620170318705
1103	34-35	0.368150625072019
1103	36-37	0.34305318379895766
1103	38-39	0.37399475352848555
1103	40-41	0.3155886311601819
1103	42-43	0.41233967504374647
1103	44-45	0.352201680682505
1103	46-47	0.37526055254561186
1103	48-49	0.2912675628727257
1103	50-51	0.343821909667291
1103	52-53	0.383111205945994
1103	54-55	0.23775859450544345
1103	56-57	0.20196686465608593
1103	58-59	0.14841940211321258
1103	60-61	0.20131053525968667
1103	62-63	0.19076297275451992
1103	64-65	0.2337399603930237
1103	66-67	0.14419982401741294
1103	68-69	0.25450672083394466
1103	70-71	0.1791399902621862
1103	72-73	0.2716674939693391
1103	74-75	0.227467218265069
1103	76-77	0.2821931848380217
1103	78-79	0.17194990896936702
1103	80-81	0.20949340461154975
1103	82-83	0.351735652514531
1103	84-85	0.30577183655399764
1103	86-87	0.2486004375952433
1103	88-89	0.24493202015445092
1103	90-91	0.2132325702604092
1103	92-93	0.1827837755695576
1103	94-95	0.25720699233439603
1103	96-97	0.16401354813146796
1103	98-99	0.24006361219814565
1103	100	0.27748852419684766
1104	1	1.4612484904345209
1104	2	1.658272725210601
1104	3	1.8039938377022686
1104	4	0.8487104012924078
1104	5	1.5047032286773785
1104	6	0.9966400378946574
1104	7	0.5500131970650273
1104	8	0.4193367762830178
1104	9	0.34834367464340943
1104	10-11	0.25378092619202874
1104	12-13	0.20435888446059636
1104	14-15	0.28376974460360316
1104	16-17	0.27705416211466627
1104	18-19	0.2927485032261927
1104	20-21	0.35953679649003334
1104	22-23	0.2862239406778784
1104	24-25	0.3036332805307609
1104	26-27	0.19067823604996903
1104	28-29	0.27957952175840006
1104	30-31	0.1926116634269306
1104	32-33	0.23564781297909576
1104	34-35	0.20226453670171196
1104	36-37	0.1798874832791455
1104	38-39	0.24117908934410792
1104	40-41	0.23253712245417546
1104	42-43	0.19598187473932427
1104	44-45	0.19488799014799696
1104	46-47	0.2658424366586587
1104	48-49	0.17610994557309567
1104	50-51	0.12960627411173675
1104	52-53	0.14340011300514277
1104	54-55	0.228355105877462
1104	56-57	0.177653071570532
1104	58-59	0.24435789223824855
1104	60-61	0.25265385480662417
1104	62-63	0.16422247134703838
1104	64-65	0.21839095262019725
1104	66-67	0.3128604371200865
1104	68-69	0.2765321147322197
1104	70-71	0.21664135989859545
1104	72-73	0.17418852532768625
1104	74-75	0.1492003187831088
1104	76-77	0.3229146879311884
1104	78-79	0.21742412668951516
1104	80-81	0.23525504453711577
1104	82-83	0.31155826514289586
1104	84-85	0.25993453165660796
1104	86-87	0.1573604870639116
1104	88-89	0.20951707602493386
1104	90-91	0.16562999860973093
1104	92-93	0.22709654924885214
1104	94-95	0.15064393885249672
1104	96-97	0.16810776904412705
1104	98-99	0.16261651068451144
1104	100	0.12108260883259447
1105	1	2.29965889796652
1105	2	2.0567049370489485
1105	3	1.9883417846768303
1105	4	0.9595872746426792
1105	5	1.5648730900773558
1105	6	1.0554412034738547
1105	7	0.48906416097268135
1105	8	0.3105762376873855
1105	9	0.27028491642792574
1105	10-11	0.25137056256131274
1105	12-13	0.14185512083976448
1105	14-15	0.2375128232911763
1105	16-17	0.17698077379496624
1105	18-19	0.1404131559212729
1105	20-21	0.21718745564957942
1105	22-23	0.21820767525069584
1105	24-25	0.296530820412336
1105	26-27	0.17772545371438753
1105	28-29	0.25176611686596573
1105	30-31	0.2675096386629434
1105	32-33	0.33124220897968826
1105	34-35	0.3641754865975315
1105	36-37	0.3616840337354432
1105	38-39	0.3879212662260514
1105	40-41	0.2302401338996063
1105	42-43	0.3358338542301098
1105	44-45	0.35428251821830514
1105	46-47	0.3764585729428873
1105	48-49	0.30887073294270095
1105	50-51	0.3280537050632475
1105	52-53	0.2866011388318981
1105	54-55	0.32179453399002256
1105	56-57	0.2006156768192966
1105	58-59	0.2510880328504115
1105	60-61	0.3324748107205835
1105	62-63	0.2843001034579018
1105	64-65	0.2560044786070179
1105	66-67	0.2611549635440866
1105	68-69	0.20957240696846213
1105	70-71	0.19627043059978888
1105	72-73	0.21057705904295432
1105	74-75	0.32432690997614344
1105	76-77	0.2965813417933134
1105	78-79	0.23526623204895003
1105	80-81	0.2706958591986819
1105	82-83	0.3504449650647885
1105	84-85	0.3661839531656774
1105	86-87	0.3505137153634159
1105	88-89	0.3413980084151227
1105	90-91	0.3539769504884305
1105	92-93	0.3133415440447216
1105	94-95	0.28303577248124867
1105	96-97	0.3087719223620624
1105	98-99	0.27977170722509115
1105	100	0.3153005897026091
1106	1	1.6875182119668963
1106	2	1.1404024628641238
1106	3	1.5698455118485732
1106	4	0.8238750758792364
1106	5	1.5293782709710868
1106	6	1.004038808225772
1106	7	0.529912605990198
1106	8	0.41224791082495926
1106	9	0.2559149995436769
1106	10-11	0.24021655018146504
1106	12-13	0.21591964895160487
1106	14-15	0.28978155826730045
1106	16-17	0.27908159852324843
1106	18-19	0.28195260107809617
1106	20-21	0.3387603328404296
1106	22-23	0.28943094480361253
1106	24-25	0.41043808223588485
1106	26-27	0.3124747313181402
1106	28-29	0.3005237024730434
1106	30-31	0.3016174569726999
1106	32-33	0.312003644985559
1106	34-35	0.2268621934350321
1106	36-37	0.17918470344766746
1106	38-39	0.24959779700960638
1106	40-41	0.2994205847320117
1106	42-43	0.23862389319842947
1106	44-45	0.27930918169416685
1106	46-47	0.24263724859143565
1106	48-49	0.2863914404445467
1106	50-51	0.19183677545268552
1106	52-53	0.19245128045371018
1106	54-55	0.19850690777159485
1106	56-57	0.24799908438650675
1106	58-59	0.3490023168298251
1106	60-61	0.3448364481758901
1106	62-63	0.3687800674541748
1106	64-65	0.2561809254533003
1106	66-67	0.24009349646356526
1106	68-69	0.21174263174174257
1106	70-71	0.2842140033269658
1106	72-73	0.14919759474129535
1106	74-75	0.270680889495452
1106	76-77	0.28789576364415126
1106	78-79	0.29617329027997386
1106	80-81	0.31347396835998964
1106	82-83	0.35079082231429126
1106	84-85	0.22813969143856738
1106	86-87	0.22731184802715632
1106	88-89	0.18420833519896718
1106	90-91	0.20830668463946722
1106	92-93	0.20631170881178562
1106	94-95	0.18775858305805926
1106	96-97	0.17675210602394031
1106	98-99	0.22118146037809439
1106	100	0.14673320236606102
1107	1	1.7345430772413835
1107	2	1.1644107263799803
1107	3	1.3857991674964119
1107	4	0.6615000352954041
1107	5	1.3981014413394917
1107	6	0.9125381195282856
1107	7	0.485880019930768
1107	8	0.35109225693668833
1107	9	0.35163035746212046
1107	10-11	0.13112827691266915
1107	12-13	0.08871081028047456
1107	14-15	0.19116241915268262
1107	16-17	0.18556132596499708
1107	18-19	0.2162410250532787
1107	20-21	0.13078201759504537
1107	22-23	0.09079715921349418
1107	24-25	0.15046509921853612
1107	26-27	0.09244036690044766
1107	28-29	0.1385308011030375
1107	30-31	0.03190524384754667
1107	32-33	0.12822909310152397
1107	34-35	0.06924711505218539
1107	36-37	0.12973776091010336
1107	38-39	0.12056348653762683
1107	40-41	0.1612759956937566
1107	42-43	0.04502193344591632
1107	44-45	0.03707327266400284
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2208	96-97	0.7982911840202043
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2216	3	-5.8426217321945835
2216	4	-2.975309654519826
2216	5	-4.197428915604508
2216	6	-2.049459518757402
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2216	12-13	-0.7467057966184143
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2216	16-17	-1.1289643970092413
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2216	34-35	-1.1867708066453844
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2216	40-41	-1.0962505034133514
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2216	54-55	-0.9589154278470673
2216	56-57	-0.7091496173757648
2216	58-59	-0.6986620890696571
2216	60-61	-0.7904800285384539
2216	62-63	-0.6805888912240547
2216	64-65	-0.8093586109635922
2216	66-67	-0.847415243062045
2216	68-69	-0.7987767953259635
2216	70-71	-0.7916269707610724
2216	72-73	-0.7841963244517771
2216	74-75	-0.7306200644709584
2216	76-77	-0.8109421326576616
2216	78-79	-0.7278939360846337
2216	80-81	-0.7235052740773682
2216	82-83	-0.7499013266299386
2216	84-85	-0.7408074466315107
2216	86-87	-0.7608542664917799
2216	88-89	-0.7448954667325367
2216	90-91	-0.7351957734767964
2216	92-93	-0.7808088085558538
2216	94-95	-0.764522149226714
2216	96-97	-0.8500391054047505
2216	98-99	-0.8990597566672172
2216	100	-0.9704164938145716
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3682.0
3	1793.0
4	1946.0
5	2589.0
6	2322.0
7	1844.0
8	1866.0
9	2115.0
10	2461.0
11	4641.0
12	3557.0
13	4723.0
14	4694.0
15	4926.0
16	5894.0
17	7671.0
18	10821.0
19	18756.0
20	40371.0
21	55028.0
22	24816.0
23	15433.0
24	14391.0
25	15200.0
26	15657.0
27	17011.0
28	19694.0
29	23636.0
30	27982.0
31	34618.0
32	44984.0
33	61000.0
34	87962.0
35	162548.0
36	249009.0
37	465882.0
38	660974.0
39	252775.0
40	1559.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	13.857367296387539	40.86574972931376	27.808064513413072	17.468818460885625
2	17.97217309150495	38.30816404462309	25.956231582998022	17.763431280873938
3	19.352532847308034	25.040316286685925	20.748761691512772	34.85838917449327
4	32.116545097232404	26.11153254059712	23.014720020060324	18.757202342110148
5	17.766134824057747	37.2943637978468	24.344095141808566	20.595406236286887
6	35.51257956497538	22.765733028557772	24.711264704979026	17.01042270148782
7	19.51308275598896	22.93107923954206	38.6982078237788	18.857630180690172
8	34.34876101834754	23.12129049141483	24.22826864846512	18.30167984177251
9	34.02875509449347	25.24891336405491	23.78061376681809	16.941717774633535
10-11	17.235070562442175	41.1285026154573	23.9999394151288	17.63648740697172
12-13	17.086406227451594	24.744144619453383	31.88827897313692	26.281170179958103
14-15	17.427196127953565	32.61710655911169	23.8598579368916	26.095839376043145
16-17	25.941275515098273	24.31322333799501	31.84117655968375	17.904324587222973
18-19	17.775685355837247	24.538282275853856	31.988980285093895	25.697052083215006
20-21	26.08400501089164	32.86806229745805	23.73536517766893	17.312567513981374
22-23	25.634500574294332	24.751202231543694	32.002015289271846	17.612281904890125
24-25	17.485845649101684	32.78821674742546	23.738814412972566	25.987123190500295
26-27	17.92896313730506	28.371619250696114	36.158502772254394	17.540914839744424
28-29	25.98619114665738	24.471365699003517	32.01865021940135	17.523792934937752
30-31	23.482254168491675	26.062324257299725	32.4241311163088	18.0312904578998
32-33	19.84478714136925	27.172684455426527	33.661891269376625	19.3206371338276
34-35	19.75541761016768	27.01473754894214	33.8222078049938	19.407637035896375
36-37	19.811257838572583	27.076570864987943	33.9969625038158	19.11520879262367
38-39	20.229714615609247	26.937038872903756	33.699086140456146	19.134160371030845
40-41	19.486003348273922	27.828961180975508	33.559524846355124	19.125510624395446
42-43	19.497389098484245	27.12108267472612	33.940541585609616	19.44098664118002
44-45	19.405387973334662	27.616681886752048	33.4536214441852	19.52430869572809
46-47	20.004779571706617	27.1099116368266	33.63369042680397	19.25161836466281
48-49	19.533297023039786	27.351265064511782	33.646325539242575	19.469112373205867
50-51	19.926668318887618	27.798252881533653	33.05730163144898	19.21777716812975
52-53	19.978605238245503	27.631240138845058	32.78986010308194	19.600294519827496
54-55	20.157384109241157	28.353563294122992	31.45967692227753	20.02937567435832
56-57	20.52124321703286	28.99337033659505	30.329800247599998	20.155586198772095
58-59	21.31497541350022	29.32078150884851	29.31825711956665	20.045985958084618
60-61	20.944186018135028	29.555641156129486	29.26940050402422	20.230772321711267
62-63	20.478494405370945	29.773621001616256	29.04961884492457	20.69826574808823
64-65	20.676210302203437	29.687256416918512	29.41249733077649	20.224035950101563
66-67	20.721385048521384	29.254294299247537	29.794208571052284	20.230112081178802
68-69	20.97869774581369	29.39907580549333	29.542792174895098	20.079434273797883
70-71	20.054035255879572	29.99015609086355	29.667888511996043	20.28792014126083
72-73	20.102417855763413	29.34322898565847	29.91344424744208	20.640908911136037
74-75	20.266814261032078	29.677006897343873	29.36311642894002	20.69306241268403
76-77	20.80828739986448	29.23701669929922	29.597955835287888	20.356740065548415
78-79	20.625039186981127	29.682039465129794	29.063106083208112	20.629815264680968
80-81	20.821849909156473	30.110379725104707	28.784880506468447	20.282889859270373
82-83	20.810863348128425	30.05546792197044	28.79230307372988	20.341365656171252
84-85	20.73823480097706	30.315516297962297	28.35456038533721	20.591688515723433
86-87	20.683269080065006	30.50954857915249	28.385351306716878	20.421831034065626
88-89	21.476955662046247	30.127273419590374	28.068439309665976	20.3273316086974
90-91	21.053665753064998	30.588418941764832	27.97123206632462	20.386683238845546
92-93	20.715835369183942	30.698053409835875	27.880332378734696	20.705778842245493
94-95	20.97458175362106	30.852141034776498	27.658697574432928	20.51457963716952
96-97	20.958682742191648	30.354100136183458	27.947967946289058	20.739249175335836
98-99	21.362171527552945	30.58229868706739	27.31865842778366	20.736871357596005
100	20.944554374630012	31.089327029516216	27.302661058474115	20.663457537379653
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	32247.0
1	17448.0
2	1999.0
3	1159.0
4	939.5
5	860.0
6	843.0
7	954.5
8	921.0
9	877.0
10	940.0
11	1326.5
12	10644.5
13	12737.5
14	5928.5
15	6237.0
16	7138.5
17	8224.5
18	9308.0
19	10509.5
20	11776.5
21	13334.5
22	15237.0
23	17533.0
24	20021.5
25	22565.0
26	26261.0
27	30459.0
28	33720.0
29	36414.5
30	39792.5
31	43758.0
32	47713.5
33	51858.0
34	56601.0
35	60923.0
36	64514.5
37	68237.0
38	71200.5
39	72894.0
40	74220.0
41	76115.5
42	77458.5
43	78031.0
44	78978.0
45	79185.5
46	78764.5
47	79414.5
48	78852.5
49	80670.5
50	93418.0
51	89987.5
52	69517.5
53	60761.5
54	57066.5
55	53098.0
56	47340.0
57	40696.5
58	36092.5
59	30255.5
60	23712.5
61	18861.5
62	14555.5
63	10645.0
64	7768.0
65	5651.5
66	3983.5
67	2987.5
68	2283.0
69	1658.5
70	1232.0
71	975.5
72	752.0
73	541.0
74	397.0
75	293.0
76	225.0
77	159.5
78	102.0
79	73.5
80	47.0
81	26.5
82	16.0
83	11.5
84	6.0
85	3.0
86	2.0
87	1.0
88	2.5
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05835501135755971
2	0.06933601926262321
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.2621848166739663E-4
18-19	0.0
20-21	3.576190313909571E-4
22-23	4.207282722246554E-5
24-25	0.0
26-27	0.0027347337694602603
28-29	9.466386125054748E-4
30-31	0.005553613193365452
32-33	0.001283221230285199
34-35	0.0018932772250109496
36-37	0.008519747512549272
38-39	0.013337086229521577
40-41	0.003534117486687106
42-43	0.006794761596428185
44-45	0.001577731020842458
46-47	0.0020615685339008117
48-49	0.003975882172522994
50-51	0.009760895915612007
52-53	0.0034078990050197093
54-55	0.0014515125391750612
56-57	5.679831675032848E-4
58-59	7.783473036156126E-4
60-61	0.006710615941983254
62-63	0.0017880951569547857
64-65	0.008561820339771738
66-67	0.010518206805616387
68-69	0.011570027486178025
70-71	0.00986607798366817
72-73	0.004081064240579158
74-75	0.0019563864658446476
76-77	0.0048173387169723044
78-79	0.016997422197876078
80-81	0.009129803507275023
82-83	0.0059743414655901075
84-85	0.004522828926415046
86-87	0.007089271386985444
88-89	0.00410210065419039
90-91	0.00660543387392709
92-93	0.011149299213953369
94-95	0.008898402957551461
96-97	0.010833753009784878
98-99	0.002208823429179441
100	0.002945097905572588
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2376831.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.7981546722347
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.8910879792199	63.64123150408268
2	7.41511283839853	10.499526112900247
3	1.1153693480384914	2.368982748572961
4	0.37729859511675956	1.068481771787728
5	0.21934507182954888	0.7764613160990415
6	0.15359332887878377	0.6524474552750127
7	0.10842559217103737	0.5373432291467621
8	0.08585559351420491	0.48627340712761524
9	0.0676842940516226	0.4312730807233013
>10	0.5376904278613912	6.637982357655649
>50	0.016819715265161555	0.8062501144644049
>100	0.007893309938655784	1.2102869693675735
>500	0.0028081807601406093	1.4078993378732974
>1k	7.76730848549108E-4	1.0551525128112746
>5k	5.974852681146985E-5	0.2526630034886844
>10k+	1.7924558043440954E-4	8.16774507862374
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	149980	6.310082626825382	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31410	1.3215075030576426	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	11697	0.4921258600211795	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5973	0.2513009969997867	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4152	0.17468637862767694	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA	3011	0.12668128276684373	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2638	0.1109881182128641	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.152380627819142E-4	4.207282722246554E-5	0.0	0.0	0.0
2	7.152380627819142E-4	4.207282722246554E-5	0.0	0.0	0.0
3	7.152380627819142E-4	8.414565444493108E-5	0.0	0.0	0.0
4	7.152380627819142E-4	8.414565444493108E-5	0.0	0.0	0.0
5	7.152380627819142E-4	8.414565444493108E-5	0.0	0.0	0.0
6	7.152380627819142E-4	8.414565444493108E-5	0.0	4.207282722246554E-5	0.0
7	7.152380627819142E-4	8.414565444493108E-5	0.0	4.207282722246554E-5	0.0
8	7.152380627819142E-4	8.414565444493108E-5	0.0	4.207282722246554E-5	0.0
9	7.152380627819142E-4	8.414565444493108E-5	0.0	8.414565444493108E-5	0.0
10-11	7.573108900043797E-4	8.414565444493108E-5	0.0	8.414565444493108E-5	0.0
12-13	7.573108900043797E-4	1.2621848166739663E-4	0.0	1.0518206805616386E-4	0.0
14-15	7.573108900043797E-4	1.6829130888986217E-4	0.0	1.2621848166739663E-4	0.0
16-17	7.783473036156124E-4	1.6829130888986217E-4	0.0	1.6829130888986217E-4	0.0
18-19	7.993837172268453E-4	1.6829130888986217E-4	0.0	1.6829130888986217E-4	0.0
20-21	8.204201308380781E-4	1.8932772250109496E-4	0.0	1.6829130888986217E-4	0.0
22-23	8.624929580605437E-4	2.1036413611232773E-4	0.0	3.1554620416849157E-4	0.0
24-25	8.835293716717764E-4	2.314005497235605E-4	0.0	3.3658261777972433E-4	0.0
26-27	9.25602198894242E-4	2.5243696333479326E-4	0.0	3.7865544500218987E-4	0.0
28-29	9.887114397279402E-4	2.5243696333479326E-4	0.0	3.7865544500218987E-4	0.0
30-31	0.0011780391622290354	2.5243696333479326E-4	0.0	3.9969185861342264E-4	0.0
32-33	0.0014515125391750612	2.5243696333479326E-4	0.0	4.417646858358882E-4	0.0
34-35	0.0018091315705660183	3.3658261777972433E-4	0.0	4.8383751305835376E-4	0.0
36-37	0.002292969083624372	3.3658261777972433E-4	0.0	5.048739266695865E-4	0.0
38-39	0.0031133892144624502	3.3658261777972433E-4	0.0	5.469467538920521E-4	0.0
40-41	0.0043755740311364165	3.3658261777972433E-4	0.0	6.100559947257503E-4	0.0
42-43	0.0059743414655901075	3.3658261777972433E-4	0.0	6.310924083369831E-4	0.0
44-45	0.00797280075865722	3.3658261777972433E-4	0.0	6.310924083369831E-4	0.0
46-47	0.010581316046450084	3.3658261777972433E-4	0.0	6.310924083369831E-4	0.0
48-49	0.013778850915357466	3.3658261777972433E-4	0.0	6.310924083369831E-4	0.0
50-51	0.016660839580096357	3.3658261777972433E-4	0.0	6.942016491706814E-4	0.0
52-53	0.02088915871595414	3.3658261777972433E-4	0.0	7.152380627819142E-4	0.0
54-55	0.025769606673760145	3.3658261777972433E-4	0.0	7.36274476393147E-4	0.0
56-57	0.03283784164713436	3.7865544500218987E-4	0.0	7.573108900043797E-4	0.0
58-59	0.04177831743190828	3.9969185861342264E-4	0.0	7.993837172268453E-4	0.0
60-61	0.054210837876146854	4.62801099447121E-4	0.0	8.414565444493109E-4	0.0
62-63	0.06935705567623444	5.048739266695865E-4	0.0	9.045657852830092E-4	0.0
64-65	0.08789013606773052	5.048739266695865E-4	0.0	9.887114397279402E-4	0.0
66-67	0.11159817420758986	5.048739266695865E-4	0.0	0.001009747853339173	0.0
68-69	0.14325797669249518	5.048739266695865E-4	0.0	0.0010728570941728713	0.0
70-71	0.1826802157999454	5.259103402808192E-4	0.0	0.001114929921395337	0.0
72-73	0.2334621182574613	5.890195811145176E-4	0.0	0.0011359663350065698	0.0
74-75	0.2949515552430947	6.100559947257503E-4	0.0	0.001199075575840268	0.0
76-77	0.3697991148718609	6.731652355594487E-4	0.0	0.0012621848166739663	0.0
78-79	0.46768154740492696	6.731652355594487E-4	0.0	0.001304257643896432	0.0
80-81	0.5855065000414417	6.731652355594487E-4	0.0	0.001304257643896432	0.0
82-83	0.7299214794825547	6.731652355594487E-4	0.0	0.0013463304711188973	0.0
84-85	0.904102984183562	6.731652355594487E-4	0.0	0.0014515125391750612	0.0
86-87	1.1151402855314494	6.942016491706814E-4	0.0	0.0014935853663975269	0.0
88	1.294244311017485	7.152380627819142E-4	0.0	0.0015146217800087595	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	1750	0.0	80.39556	1
ATCGAGT	700	0.0	73.942406	1
AACGAGT	45905	0.0	71.37323	1
ACGAGTG	48935	0.0	70.21729	2
GTGGAAT	51875	0.0	69.7124	6
TGGAATC	52560	0.0	69.21517	7
CGAGTGG	50585	0.0	69.139694	3
GGAATCA	53055	0.0	68.68455	8
GAATCAC	53105	0.0	68.62873	9
AGTGGAA	51875	0.0	68.14512	5
GAGTGGA	52195	0.0	67.79037	4
TCGAGTG	865	0.0	67.41091	2
CGTGTGG	1185	0.0	63.05773	3
AACGTGT	1065	0.0	57.437122	1
ACGTGTG	1385	0.0	49.231472	2
CGATTGG	475	0.0	45.511776	3
GACGAGT	555	0.0	44.08687	1
ACGATTG	440	0.0	42.749584	2
AGTACTT	22625	0.0	41.034824	20-21
GAGTACT	22065	0.0	40.85157	20-21
>>END_MODULE