FastQCFastQC Report
Thu 26 May 2016
SRR2132688_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132688_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4387184
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG3668218.361194789185957No Hit
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1340073.0545105926717455No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT427170.9736769645403521No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA408790.9317822092713686No Hit
TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG340080.7751669407984713No Hit
CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA334340.7620833774010847No Hit
CTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCG333350.7598268046200023No Hit
AGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTC328370.7484755597212244No Hit
CCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATT322030.7340243764565152No Hit
CCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGAT309770.7060793438342226No Hit
CCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTC288340.657232520906349No Hit
TCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGA279950.6381086364282875No Hit
TACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACT271520.6188935772924044No Hit
TCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGT264070.6019122972731483No Hit
CGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAG262190.5976270883555375No Hit
ATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTA259150.5906978143611027No Hit
TCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACT255890.5832670797486497No Hit
CTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGC249470.5686335471682975No Hit
ACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGA249040.5676534195967162No Hit
ACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTC247530.5642115762639544No Hit
TCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCA246140.5610432569046568No Hit
TGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCA240990.5493045197101375No Hit
CTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACG234830.5352636224056251No Hit
ACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAG227200.5178720564261722No Hit
CTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGT220760.5031929365169092No Hit
GTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCAC212450.48425140135449074No Hit
ATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGG212110.4834764167630079No Hit
CAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTT209580.47770961965579745No Hit
TGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATG207660.473333236080365No Hit
GAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGT206590.4708943139836396No Hit
GATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGT205990.469526694116317No Hit
GTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCAT199430.45457405023358943No Hit
ATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCC197740.45072192094063074No Hit
AACGAGTGGAATCACTGCTGAGTACATGGTAACGAGTGGAATCACTGCTG196360.4475763952457887No Hit
CGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAG192490.43875524710155767No Hit
TGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGA185110.42193352273348916No Hit
TTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTAC183930.4192438703277547No Hit
TGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTG183860.41908431467656704No Hit
ACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATC173760.39606271357663597No Hit
ATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCAC170690.3890650585888351No Hit
ACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAAC152990.3487202725028173No Hit
CAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCC150500.3430446500534283No Hit
TGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACA147830.3369587416438426No Hit
GCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCC146470.33385880327791134No Hit
GAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATG145660.33201251645702573No Hit
GGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGC143720.3275905455526825No Hit
GGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACAT137080.312455552354312No Hit
TACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG135610.30910488367937156No Hit
AGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGA133850.3050931987352251No Hit
TACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAAT129630.2954742723350559No Hit
TCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGA127050.28959350690556857No Hit
GGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTG122030.27815108734896915No Hit
TGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACT118660.27046962242750705No Hit
CACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAA117610.26807628765969244No Hit
GAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCT108160.24653627474936088No Hit
GAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGG104700.2386496668478003No Hit
GTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTAC91850.20935980802264048No Hit
AGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCA85430.19472627544228827No Hit
GTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAA79460.18111845776242802No Hit
AACGAGTGGAATCACTGCTGAGTACATGGAAACGAGTGGAATCACTGCTG78370.1786339483367919No Hit
GCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAG71280.1624732402379294No Hit
CATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTC63920.1456971031987717No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT56180.12805480691030965No Hit
AACGTGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG55820.12723423498991607No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGTAACGAGTGGAATCACTGCT48490.11052647894412451No Hit
AACGAGTGGAATCACTGCTGAGTACTTGGGAACGAGTGGAATCACTGCTG48170.10979708168155244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT38200.081.166021
ATCGAGT14850.062.7322651
TCGAGTG19250.056.9166872
AACGTGT21550.056.3280451
CGTGTGG28300.056.2892723
ACGTGTG25300.054.829712
ATCGTGT3700.047.0492021
GTGTGGA37350.046.2987864
CGATTGG9300.042.443263
TGTGGAA41700.041.9198075
ATCTGCG5150.040.15842494
TACGTGT3900.039.810861
ATGAGTG23150.039.609382
AATGAGT21750.038.720951
TGAGTGG27100.036.933693
TCGTGTG5350.036.91562
ACGATTG9450.036.822592
AGTACTT269600.034.2071620-21
GTACTTT248350.033.90907722-23
GAGTACT269000.033.60215820-21
Produced by FastQC (version 0.11.5)