FastQCFastQC Report
Thu 26 May 2016
SRR2132687_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132687_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202474
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT650925.413173174638287No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA218591.8178355623489573No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG140511.1685075935113773No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25690.21364287294361456No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT14680.12208164168206548No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12800.10644720800616063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT7950.084.626131
GTGGAAT292400.057.877736
TGGAATC294650.057.675027
GAATCAC295400.057.5285879
ACGTGTG6050.057.5168382
GGAATCA296800.057.304738
AGTGGAA291750.056.52475
AACGAGT274450.055.8843151
ACGAGTG288500.055.711512
AACGTGT5500.055.6014631
CGAGTGG294800.055.3978773
CGTGTGG6800.055.290033
ATCGAGT3350.054.7715951
GAGTGGA303700.054.3315124
TCGAGTG3800.053.2112582
CGATTGG1700.044.2320253
GAGTACT97800.041.3261920-21
AGTACTT100950.040.4789320-21
GTACTTT99200.039.89019822-23
TACTTTT106700.037.68091222-23
Produced by FastQC (version 0.11.5)