##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2132687_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1202474 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.181228034868113 31.0 28.0 34.0 16.0 34.0 2 30.296914527881682 33.0 30.0 34.0 19.0 34.0 3 30.748933448872908 33.0 31.0 34.0 25.0 34.0 4 35.20389713207936 37.0 35.0 37.0 32.0 37.0 5 34.68276985614658 37.0 35.0 37.0 32.0 37.0 6 35.22949934884247 37.0 35.0 37.0 32.0 37.0 7 35.65131221132432 37.0 35.0 37.0 33.0 37.0 8 35.85463635804184 37.0 35.0 37.0 35.0 37.0 9 37.77538724330007 39.0 39.0 39.0 35.0 39.0 10-11 37.87354445917334 39.0 39.0 39.0 35.0 39.0 12-13 37.881164166543314 39.0 39.0 39.0 35.0 39.0 14-15 39.32024185138307 41.0 39.0 41.0 37.0 41.0 16-17 39.27496270189626 41.0 39.0 41.0 36.5 41.0 18-19 39.21335762769091 41.0 39.0 41.0 36.0 41.0 20-21 39.09816470044258 41.0 39.0 41.0 35.5 41.0 22-23 39.024211334299125 40.5 39.0 41.0 35.0 41.0 24-25 38.93225383667339 41.0 39.0 41.0 35.0 41.0 26-27 38.88724163682541 41.0 39.0 41.0 35.0 41.0 28-29 38.73597724358281 41.0 39.0 41.0 35.0 41.0 30-31 38.61559875722885 41.0 39.0 41.0 35.0 41.0 32-33 38.487878739997704 40.0 38.0 41.0 35.0 41.0 34-35 38.40907620455827 40.0 38.0 41.0 35.0 41.0 36-37 38.265555429888714 40.0 38.0 41.0 34.5 41.0 38-39 37.92367444119374 40.0 37.0 41.0 34.0 41.0 40-41 37.767488527818486 40.0 37.0 41.0 33.5 41.0 42-43 37.64516239020553 40.0 36.5 41.0 33.0 41.0 44-45 37.51605398536684 40.0 36.0 41.0 33.0 41.0 46-47 37.39041966811756 40.0 36.0 41.0 33.0 41.0 48-49 37.253259945745185 40.0 35.5 41.0 33.0 41.0 50-51 37.01954221047607 40.0 35.0 41.0 33.0 41.0 52-53 36.61789776743613 40.0 35.0 41.0 31.5 41.0 54-55 35.95587846390026 39.0 35.0 41.0 29.0 41.0 56-57 35.452551988650065 39.0 35.0 41.0 27.0 41.0 58-59 35.19489236357709 39.0 35.0 41.0 26.0 41.0 60-61 34.95133782518374 39.0 35.0 41.0 26.0 41.0 62-63 34.66447008417646 38.5 35.0 40.5 25.0 41.0 64-65 34.38414219351104 38.0 35.0 40.0 23.5 41.0 66-67 34.04845718077896 37.0 34.5 40.0 23.0 41.0 68-69 33.75173392522416 37.0 34.0 40.0 22.5 41.0 70-71 33.41617074464811 36.0 34.0 39.0 22.0 41.0 72-73 33.060966806766714 36.0 34.0 39.0 21.5 41.0 74-75 32.69590485948137 35.0 34.0 38.5 20.0 40.0 76-77 32.31369077418722 35.0 33.0 37.5 18.5 39.5 78-79 32.17761423531819 35.0 33.5 37.0 18.5 39.0 80-81 32.02748957565819 35.0 34.0 37.0 18.0 39.0 82-83 31.69023696146445 35.0 34.0 36.0 12.5 38.5 84-85 31.392922840743335 35.0 33.0 36.0 8.5 37.0 86-87 31.20223223121664 35.0 33.0 36.0 4.5 37.0 88-89 30.987948595977958 35.0 33.0 35.0 2.0 36.5 90-91 30.776683737028826 35.0 33.0 35.0 2.0 36.0 92-93 30.616400021954735 35.0 33.0 35.0 2.0 36.0 94-95 30.42736267062739 35.0 33.0 35.0 2.0 36.0 96-97 30.224097984654968 35.0 33.0 35.0 2.0 36.0 98-99 30.07898507576879 35.0 33.0 35.0 2.0 35.0 100 29.989742813566032 35.0 33.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 2.9577302962801646 1101 2 2.3740669880477725 1101 3 2.140325881709895 1101 4 1.0943598329644146 1101 5 1.6583155207019757 1101 6 1.1344670795701504 1101 7 0.6002315795567483 1101 8 0.4477295551259388 1101 9 0.4046638614662612 1101 10-11 0.33158183760010473 1101 12-13 0.30100861818748825 1101 14-15 0.4562749235109038 1101 16-17 0.44002596595318977 1101 18-19 0.46006240261844766 1101 20-21 0.4683379242855068 1101 22-23 0.44782258640264416 1101 24-25 0.45911310304395414 1101 26-27 0.4069248767900575 1101 28-29 0.412875154353479 1101 30-31 0.44932609897998077 1101 32-33 0.46265885386721806 1101 34-35 0.41535190153921064 1101 36-37 0.42298170527828916 1101 38-39 0.3984966100083298 1101 40-41 0.3325647383051802 1101 42-43 0.292414667923552 1101 44-45 0.27011828503549395 1101 46-47 0.24762766879456422 1101 48-49 0.2758451032954099 1101 50-51 0.26638024423538553 1101 52-53 0.2142007239178767 1101 54-55 0.13531993804672027 1101 56-57 0.14596709864671453 1101 58-59 0.013498008736945621 1101 60-61 0.082138830086663 1101 62-63 0.1004044484614326 1101 64-65 0.08630556140474255 1101 66-67 0.12976732981960737 1101 68-69 0.2604424855980767 1101 70-71 0.11402477534127087 1101 72-73 0.1443160095376328 1101 74-75 0.20205758355877634 1101 76-77 0.1643318473258546 1101 78-79 0.11948181165517013 1101 80-81 0.04723352294053029 1101 82-83 0.11150931901300254 1101 84-85 0.056921863858306665 1101 86-87 0.11180507879009838 1101 88-89 0.0916443039171746 1101 90-91 0.037282566300135045 1101 92-93 0.09434893818681189 1101 94-95 0.17688693187814408 1101 96-97 0.2561528496019285 1101 98-99 0.11636556198199699 1101 100 0.14963258032897642 1102 1 2.7563216153987575 1102 2 2.319497008376949 1102 3 2.0926866416893866 1102 4 1.0354408079726483 1102 5 1.5253803933165102 1102 6 1.0587080781513123 1102 7 0.5904393407966069 1102 8 0.5533437885227457 1102 9 0.4701090853472252 1102 10-11 0.4820799767745072 1102 12-13 0.38797219156690943 1102 14-15 0.5725056435996478 1102 16-17 0.47070946960426596 1102 18-19 0.5338363067370295 1102 20-21 0.5370943777475361 1102 22-23 0.5765810703064389 1102 24-25 0.5557101321609892 1102 26-27 0.572789529274992 1102 28-29 0.6441290078595827 1102 30-31 0.6119282710574439 1102 32-33 0.6260289414981131 1102 34-35 0.6482946430813001 1102 36-37 0.7404519543540644 1102 38-39 0.6753714877484995 1102 40-41 0.5801535887037161 1102 42-43 0.6707590159907895 1102 44-45 0.6472304397056377 1102 46-47 0.70161587737541 1102 48-49 0.6409010456803941 1102 50-51 0.5444427446996585 1102 52-53 0.4523898050934534 1102 54-55 0.24571716178902392 1102 56-57 0.22283200988916718 1102 58-59 0.45219801220355293 1102 60-61 0.4687552164945856 1102 62-63 0.4532833115626076 1102 64-65 0.4701517566787601 1102 66-67 0.4168052495577328 1102 68-69 0.44565034588387675 1102 70-71 0.32535708561257337 1102 72-73 0.38007273909021677 1102 74-75 0.3823399202010691 1102 76-77 0.39215762799356924 1102 78-79 0.3709772505896325 1102 80-81 0.46120020760054814 1102 82-83 0.6880636283780461 1102 84-85 0.5900781113047842 1102 86-87 0.5325067326096793 1102 88-89 0.5891912145504961 1102 90-91 0.5631872581063142 1102 92-93 0.6276310049289187 1102 94-95 0.6145938450371382 1102 96-97 0.5170468196851026 1102 98-99 0.5426414903519721 1102 100 0.5705236595087797 1103 1 2.5192732612346944 1103 2 2.0956040739629245 1103 3 1.9985223449388378 1103 4 0.9909204104385907 1103 5 1.5631310443177568 1103 6 0.9599321755411623 1103 7 0.5003023617149722 1103 8 0.42709664824845106 1103 9 0.382769359475013 1103 10-11 0.3233035228919263 1103 12-13 0.16663813047320275 1103 14-15 0.30676068221445973 1103 16-17 0.3767835527428147 1103 18-19 0.3381241460611051 1103 20-21 0.4298929425006932 1103 22-23 0.4361727410041567 1103 24-25 0.4280095409364577 1103 26-27 0.4434307287710979 1103 28-29 0.4853996544506458 1103 30-31 0.4909329093182322 1103 32-33 0.502167720393345 1103 34-35 0.47518434891688344 1103 36-37 0.39459151048284014 1103 38-39 0.4311025249615099 1103 40-41 0.42063594601729903 1103 42-43 0.4469743672315971 1103 44-45 0.3522388102624774 1103 46-47 0.4277181420449381 1103 48-49 0.41849836419439157 1103 50-51 0.4015314265618528 1103 52-53 0.41504094687859805 1103 54-55 0.2060264559089191 1103 56-57 0.08676252775509141 1103 58-59 0.22833400444697105 1103 60-61 0.2337576260397185 1103 62-63 0.23308117632094394 1103 64-65 0.17382253436361594 1103 66-67 0.22979986132412478 1103 68-69 0.29252322276565934 1103 70-71 0.21111117631142662 1103 72-73 0.09610004585484688 1103 74-75 0.11864117907102667 1103 76-77 0.0510388842162115 1103 78-79 -0.08677423034921361 1103 80-81 -0.06192612495667049 1103 82-83 0.03595120999740686 1103 84-85 0.020083778506464256 1103 86-87 0.025505269920373053 1103 88-89 0.03284299453948947 1103 90-91 -0.015263654264696669 1103 92-93 0.04516625380266959 1103 94-95 0.06912401705534066 1103 96-97 0.10958289395300724 1103 98-99 0.10108636295806761 1103 100 0.17633086015649369 1104 1 1.4293077880881526 1104 2 1.6652777990691305 1104 3 1.7731740239612002 1104 4 0.864884292145554 1104 5 1.4731456186165985 1104 6 0.9775407516851757 1104 7 0.5168367757024868 1104 8 0.3794564994202574 1104 9 0.38633103937290514 1104 10-11 0.26818503747545464 1104 12-13 0.2117935906535635 1104 14-15 0.28597175820510756 1104 16-17 0.30196013285112855 1104 18-19 0.3063760580816606 1104 20-21 0.39758231160091384 1104 22-23 0.22246435324800018 1104 24-25 0.20404330661484948 1104 26-27 0.23156378312602754 1104 28-29 0.37916465981400904 1104 30-31 0.36033078291195153 1104 32-33 0.37540601405087415 1104 34-35 0.2257701201843787 1104 36-37 0.33936566841057925 1104 38-39 0.3324529257696156 1104 40-41 0.2721179145661168 1104 42-43 0.30918455637586106 1104 44-45 0.25436661620214807 1104 46-47 0.26611629735681674 1104 48-49 0.24510649542621366 1104 50-51 0.23746901497103323 1104 52-53 0.2368090946204191 1104 54-55 0.1986650055547443 1104 56-57 0.21664785119195784 1104 58-59 0.248763037975003 1104 60-61 0.19337886699331364 1104 62-63 0.1279447633852584 1104 64-65 0.11675629247790198 1104 66-67 0.11682626361645987 1104 68-69 0.08373969130644099 1104 70-71 0.08337015689669869 1104 72-73 0.0011526878410990093 1104 74-75 0.042247314451792306 1104 76-77 0.08532883831341564 1104 78-79 0.14414457585668572 1104 80-81 0.02949380008128344 1104 82-83 0.10869658621622591 1104 84-85 0.0808223327383999 1104 86-87 0.04758664298966764 1104 88-89 0.0083277076068633 1104 90-91 0.02629364560075942 1104 92-93 -0.006952350137929386 1104 94-95 -0.09854117073988888 1104 96-97 -0.053981448270828736 1104 98-99 0.04137000490083764 1104 100 0.1016484482440454 1105 1 2.1392063314557532 1105 2 1.957943873494166 1105 3 1.8725172702914143 1105 4 0.9690201128937517 1105 5 1.564987137575386 1105 6 1.030274933898184 1105 7 0.5199654331471066 1105 8 0.34345490600824036 1105 9 0.2607317472426658 1105 10-11 0.25252412401324165 1105 12-13 0.14486235413763637 1105 14-15 0.2512004762839837 1105 16-17 0.16608298068732807 1105 18-19 0.2483258021486705 1105 20-21 0.3317668160215703 1105 22-23 0.3087157104684195 1105 24-25 0.3333511594569032 1105 26-27 0.26491703410956546 1105 28-29 0.2683194111430325 1105 30-31 0.26751277030566456 1105 32-33 0.3724862275398877 1105 34-35 0.38675232473335086 1105 36-37 0.3305208216178528 1105 38-39 0.43481025899014014 1105 40-41 0.4089944744882814 1105 42-43 0.3870740257555596 1105 44-45 0.34823691528417555 1105 46-47 0.4429048318407922 1105 48-49 0.3688374062092379 1105 50-51 0.4168944348280448 1105 52-53 0.4034757612870834 1105 54-55 0.505269028567561 1105 56-57 0.44176136417191714 1105 58-59 0.34098087713909564 1105 60-61 0.2746569808447248 1105 62-63 0.3263959457533758 1105 64-65 0.3039945428268993 1105 66-67 0.3354920983246501 1105 68-69 0.31620510862527595 1105 70-71 0.4078110525916685 1105 72-73 0.42089425192305185 1105 74-75 0.39921655313927573 1105 76-77 0.33839227867327537 1105 78-79 0.35443706566871924 1105 80-81 0.3034443338038102 1105 82-83 0.3797061819408114 1105 84-85 0.3423243350791658 1105 86-87 0.32871634390764015 1105 88-89 0.2895094059302572 1105 90-91 0.3313333398912697 1105 92-93 0.39206868854109445 1105 94-95 0.3498262869923856 1105 96-97 0.3516044831701528 1105 98-99 0.3548332788689983 1105 100 0.3619333587732534 1106 1 1.8116031158193628 1106 2 1.2337588957546828 1106 3 1.541246710940289 1106 4 0.8457734280356917 1106 5 1.5094119202703098 1106 6 1.0530022066254574 1106 7 0.5660346219888055 1106 8 0.39128168428525356 1106 9 0.35393442397614905 1106 10-11 0.26926824087583867 1106 12-13 0.2567937021471778 1106 14-15 0.3082494699443572 1106 16-17 0.27236072085133145 1106 18-19 0.1923000743219916 1106 20-21 0.29979039986269385 1106 22-23 0.20705036028410717 1106 24-25 0.27230484663333954 1106 26-27 0.21408072586805105 1106 28-29 0.21144523608165144 1106 30-31 0.1700922208708988 1106 32-33 0.15304699773335528 1106 34-35 0.11963719920590421 1106 36-37 0.1492497493515188 1106 38-39 0.04703183151976731 1106 40-41 0.183784521197758 1106 42-43 0.18434386360596733 1106 44-45 0.27055248807367605 1106 46-47 0.20276690894220195 1106 48-49 0.12752875435556632 1106 50-51 0.05434245942061722 1106 52-53 0.07527670915438023 1106 54-55 -0.053183215607433 1106 56-57 -0.15599941901540149 1106 58-59 -0.10577809716726705 1106 60-61 -0.09747496830144087 1106 62-63 0.10004005440933383 1106 64-65 0.14540543577808052 1106 66-67 -0.008688536732762486 1106 68-69 0.11147158517137257 1106 70-71 0.2149575001409616 1106 72-73 0.1410857573633706 1106 74-75 0.2266611924914983 1106 76-77 0.10166994360970705 1106 78-79 0.09643259646756519 1106 80-81 0.08872264159396082 1106 82-83 0.12019972604809581 1106 84-85 0.15621946443637924 1106 86-87 0.21360192962260882 1106 88-89 0.16758305029537013 1106 90-91 0.06804028749868962 1106 92-93 0.1399477841690029 1106 94-95 0.14134249853229264 1106 96-97 0.11951334497711841 1106 98-99 0.23510946660963938 1106 100 0.17145460372103827 1107 1 1.4886922338010464 1107 2 1.0736550248821253 1107 3 1.3931044568020035 1107 4 0.6665939626187054 1107 5 1.3642159343120497 1107 6 0.9069721261777488 1107 7 0.49008201645886373 1107 8 0.3803326400265874 1107 9 0.3093041524520075 1107 10-11 0.14355688453708382 1107 12-13 0.14340506274068332 1107 14-15 0.20906820317060237 1107 16-17 0.16979306912984526 1107 18-19 0.2298003436966667 1107 20-21 0.19068039719381602 1107 22-23 0.15444411200741115 1107 24-25 0.16884729151652067 1107 26-27 0.09320733859347285 1107 28-29 0.12786269664381678 1107 30-31 0.03147698901804574 1107 32-33 0.13928070885430088 1107 34-35 0.11364064278116359 1107 36-37 0.256985664924251 1107 38-39 0.23399249508214126 1107 40-41 0.19793677562890366 1107 42-43 0.17479694903723697 1107 44-45 0.3230294365090103 1107 46-47 0.2840538236324903 1107 48-49 0.24422351887237426 1107 50-51 0.3219393942407933 1107 52-53 0.3699850302622494 1107 54-55 0.3183459263113022 1107 56-57 0.4333866940711246 1107 58-59 0.4597448737231318 1107 60-61 0.3372163172760452 1107 62-63 0.5103527942563915 1107 64-65 0.5169873475038642 1107 66-67 0.4678271032986885 1107 68-69 0.5120494007444876 1107 70-71 0.39574105717959895 1107 72-73 0.39820360311750136 1107 74-75 0.3987938165544165 1107 76-77 0.4057484971312846 1107 78-79 0.3907966705618975 1107 80-81 0.4230472737110489 1107 82-83 0.273601002580115 1107 84-85 0.2969262869922389 1107 86-87 0.3691826544421595 1107 88-89 0.23838383073595182 1107 90-91 0.1520067203757698 1107 92-93 0.28484690542738633 1107 94-95 0.2641132504661279 1107 96-97 0.2520223365654424 1107 98-99 0.3021192475348151 1107 100 0.29493429452864817 1108 1 0.6758138948767858 1108 2 0.2931299309600135 1108 3 0.12288007674230528 1108 4 0.1989934211054063 1108 5 1.1504888983362136 1108 6 0.8321041064527464 1108 7 0.44643908902490637 1108 8 0.39787419138607305 1108 9 0.28931383009935985 1108 10-11 0.1817566790230032 1108 12-13 0.1179571997065807 1108 14-15 0.15482798004102705 1108 16-17 0.13521695552078938 1108 18-19 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2214 46-47 -0.5546445274021181 2214 48-49 -0.5250947369164507 2214 50-51 -0.4944975334187589 2214 52-53 -0.5366813933862034 2214 54-55 -0.8309849056702419 2214 56-57 -0.7786217473402246 2214 58-59 -0.6856528386075169 2214 60-61 -0.6214732330274302 2214 62-63 -0.6897858563155239 2214 64-65 -0.6675846843974824 2214 66-67 -0.6338071508133112 2214 68-69 -0.6784502360300664 2214 70-71 -0.664764490145167 2214 72-73 -0.7117871795487076 2214 74-75 -0.647827666082371 2214 76-77 -0.6411480734477202 2214 78-79 -0.6983409358996049 2214 80-81 -0.6897715900572763 2214 82-83 -0.6343264252369636 2214 84-85 -0.6668421704583096 2214 86-87 -0.7056978977646651 2214 88-89 -0.646435428804903 2214 90-91 -0.7210669479928917 2214 92-93 -0.7143864154946584 2214 94-95 -0.6499994249300975 2214 96-97 -0.6210249035721382 2214 98-99 -0.71156940446917 2214 100 -0.7165379011496178 2215 1 -5.38976221655253 2215 2 -4.362785066440075 2215 3 -3.482476797450996 2215 4 -1.6803184073056912 2215 5 -2.5012559460518062 2215 6 -4.914564443060399 2215 7 -2.0713282069875945 2215 8 -1.0967911062576405 2215 9 -1.5690099790222405 2215 10-11 -0.794720849556235 2215 12-13 -0.5273267977700584 2215 14-15 -0.5735660926878836 2215 16-17 -0.5709994732825336 2215 18-19 -0.5525559871437196 2215 20-21 -0.5601669952003192 2215 22-23 -0.7520225303194934 2215 24-25 -0.8129994546089492 2215 26-27 -0.8623779942616139 2215 28-29 -0.8462457973791331 2215 30-31 -1.111680608059899 2215 32-33 -0.9895020876769038 2215 34-35 -0.874212152290724 2215 36-37 -0.7686559398105643 2215 38-39 -0.8166455148166207 2215 40-41 -0.8216846146345205 2215 42-43 -0.7202169999957349 2215 44-45 -0.8008507520154211 2215 46-47 -0.7319028384139017 2215 48-49 -1.0032190693240537 2215 50-51 -1.0606750431553351 2215 52-53 -0.9549467213075857 2215 54-55 -0.7922407188594036 2215 56-57 -0.7393139071351342 2215 58-59 -0.8152421768488622 2215 60-61 -0.7501653270901727 2215 62-63 -0.6780263703411222 2215 64-65 -0.653877882760753 2215 66-67 -0.6365333927405743 2215 68-69 -0.6882821259944123 2215 70-71 -0.670443750000878 2215 72-73 -0.6226313449965843 2215 74-75 -0.5057508580367482 2215 76-77 -0.6692551511595823 2215 78-79 -0.6877383361921119 2215 80-81 -0.6288920935657316 2215 82-83 -0.6602018956328308 2215 84-85 -0.5760430315497906 2215 86-87 -0.6693134960096181 2215 88-89 -0.6198369044922138 2215 90-91 -0.5812529700460338 2215 92-93 -0.5855907932574631 2215 94-95 -0.648587826487951 2215 96-97 -0.6814853574528037 2215 98-99 -0.617366982238547 2215 100 -0.6334688659336081 2216 1 -5.733320291208081 2216 2 -4.782850458408838 2216 3 -6.158560917894349 2216 4 -3.2531965654151307 2216 5 -4.296746673801678 2216 6 -1.972427723853869 2216 7 -1.2427346823246879 2216 8 -0.7758107076316847 2216 9 -0.6967141639628522 2216 10-11 -1.063791969333586 2216 12-13 -0.7691084856701949 2216 14-15 -1.805946776390158 2216 16-17 -1.1566000991307632 2216 18-19 -1.798221686220998 2216 20-21 -1.125236869096824 2216 22-23 -0.7984696170105465 2216 24-25 -1.4474585449258015 2216 26-27 -1.2490194730349558 2216 28-29 -2.1310950922094563 2216 30-31 -2.1640422736258884 2216 32-33 -1.4355237966474235 2216 34-35 -1.2707449072699148 2216 36-37 -1.3942690244672775 2216 38-39 -1.2028678788252094 2216 40-41 -1.2609005317129842 2216 42-43 -1.6588720470154286 2216 44-45 -1.45048576708286 2216 46-47 -1.8884852608692881 2216 48-49 -1.5857656654355452 2216 50-51 -1.5112104140369524 2216 52-53 -1.4218992844502054 2216 54-55 -1.2705158113638149 2216 56-57 -1.2293085556630885 2216 58-59 -1.0993316688951822 2216 60-61 -1.0597348439422731 2216 62-63 -1.0051283888582248 2216 64-65 -1.1559591971762302 2216 66-67 -1.1846637885985274 2216 68-69 -1.1980863079713018 2216 70-71 -1.127417942708675 2216 72-73 -1.2470771153485352 2216 74-75 -1.1676486846233303 2216 76-77 -1.0884019296796588 2216 78-79 -0.95935070620083 2216 80-81 -1.0281545466424902 2216 82-83 -1.0066999247589337 2216 84-85 -0.9939228197053112 2216 86-87 -0.9301442155840967 2216 88-89 -1.0604131970796153 2216 90-91 -1.0545943072877506 2216 92-93 -1.0548683389392934 2216 94-95 -1.1297944855756974 2216 96-97 -1.115219650434991 2216 98-99 -1.2700645547280693 2216 100 -1.3218907296885938 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1998.0 3 1077.0 4 1098.0 5 1456.0 6 1413.0 7 1066.0 8 1058.0 9 1177.0 10 1304.0 11 2594.0 12 1792.0 13 2494.0 14 2357.0 15 2410.0 16 3031.0 17 3633.0 18 4810.0 19 7899.0 20 15872.0 21 21160.0 22 10145.0 23 6992.0 24 7073.0 25 7454.0 26 8144.0 27 9060.0 28 10259.0 29 12302.0 30 14699.0 31 18359.0 32 23527.0 33 32324.0 34 46837.0 35 89670.0 36 133000.0 37 241038.0 38 326039.0 39 125009.0 40 844.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 15.222997086838182 40.60952564805577 26.529742791359865 17.637734473746182 2 18.271722469005944 38.50236585053284 25.675844358157985 17.55006732230324 3 19.18619446241665 25.335017638635016 21.64770298567786 33.83108491327047 4 31.63353220111204 26.245640238375216 23.085571912573577 19.035255647939163 5 18.243055567105817 37.88165066354865 24.259983999654047 19.615309769691486 6 34.6384204564922 24.048503335623057 24.053409886617093 17.25966632126765 7 19.15575721387739 23.94247193702317 38.22594085194358 18.675829997155862 8 33.28105223065114 24.314205546232184 24.061060779692532 18.34368144342414 9 33.165374053825694 25.205784075165035 24.26214620856667 17.366695662442595 10-11 17.53276993930846 40.59014997413665 23.91428005927779 17.962800027277098 12-13 17.441250288987536 25.238508275438804 31.418517157127724 25.901724278445936 14-15 17.69273181790209 32.73617558466961 23.737727385373823 25.83336521205448 16-17 25.6619280947914 24.90677537605865 31.323390482273183 18.107906046876767 18-19 18.032822331293648 25.064533619853734 31.36816263802793 25.534481410824682 20-21 25.816600074596423 32.78176055046673 23.7079828569599 17.69365651797695 22-23 25.41650640659142 25.21255163839506 31.38624791835156 17.98469403666196 24-25 17.798804797442607 32.75871245448966 23.65452392317838 25.78795882488935 26-27 18.180638771108477 28.841211210948988 35.02217764654566 17.955972371396886 28-29 26.033378130239242 25.0008316257285 31.063216861377967 17.902573382654282 30-31 23.50684188706094 26.659553172193 31.35495852286596 18.4786464178801 32-33 19.957911386878433 27.732745207129035 32.20743590447274 20.101907501519797 34-35 20.352745889233002 27.62118571053148 32.42528599894216 19.60078240129336 36-37 20.406357338301216 27.12842754846597 33.043272149635314 19.421942963597502 38-39 21.329433110611987 27.118011817940996 32.31552713098668 19.237027940460333 40-41 19.56180765776255 28.80097967466152 32.24626592595057 19.390946741625363 42-43 19.69865825452495 27.181594309329178 33.01244439195635 20.107303044189525 44-45 19.49946609273021 28.114126421257822 32.04574637242444 20.340661113587526 46-47 20.444392883027522 27.2927054317042 32.76491029353 19.49799139173828 48-49 19.792945320998374 27.46208530017398 32.58689027939948 20.15807909942816 50-51 20.562055436231287 28.23089350430087 31.770936211531787 19.43611484793606 52-53 20.572199379931604 27.70755991111466 31.957806075605767 19.76243463334797 54-55 20.106889343790243 28.876589088105742 30.344997353339014 20.671524214765 56-57 20.42619084448126 29.363325033722365 30.000224538573235 20.21025958322314 58-59 22.119234872973646 29.07851109427535 28.712139293635502 20.090114739115503 60-61 21.27640463642087 29.992731161941226 28.416998435619632 20.313865766018267 62-63 20.44902266694166 29.98097227674027 28.344696188467605 21.225308867850465 64-65 21.02718859860327 29.809070185024854 28.840240889104017 20.32350032726786 66-67 21.05346546840764 29.020909373309305 29.686186822449766 20.239438335833288 68-69 21.685792525840185 29.044303099990227 29.166231751767903 20.103672622401685 70-71 19.99057685766065 30.504741096461206 29.23124457836636 20.273437467511783 72-73 20.024517152234633 29.053864965214004 29.89682919537085 21.024788687180514 74-75 20.18760855436461 29.828296466257864 28.844472184094066 21.139622795283465 76-77 20.97024293913818 29.144504771833148 29.427600411004303 20.457651878024368 78-79 20.39698640004059 29.42177507292376 28.987060450825634 21.19417807621001 80-81 21.193747200848986 30.126454051091443 28.335953844624818 20.34384490343475 82-83 21.14128482179094 29.687551979028747 28.80507282054012 20.366090378640195 84-85 20.440387435822053 30.47952938182505 27.87498695337771 21.205096228975187 86-87 20.49058166699795 30.62665243943516 28.43705323318826 20.44571266037863 88-89 22.132187100809027 29.721306485522675 27.724811049606128 20.421695364062167 90-91 21.267284622367413 30.627645997959924 27.59709598800072 20.507973391671943 92-93 20.631861814120082 30.687448720278553 27.342711345226416 21.33797812037495 94-95 21.294944044498262 30.84592775685638 27.197176609136513 20.661951589508842 96-97 21.372158282835173 30.11267896143731 27.791690300859813 20.7234724548677 98-99 22.1394792222389 30.260388880549442 26.90216611647123 20.69796578074043 100 20.752543754236125 31.554195375294707 26.926828862618557 20.766432007850604 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 21844.0 1 11919.0 2 1441.5 3 764.5 4 601.0 5 561.0 6 569.5 7 628.0 8 606.5 9 555.0 10 601.0 11 923.0 12 7656.5 13 8724.0 14 3101.5 15 2961.5 16 3314.5 17 3852.0 18 4345.5 19 4727.5 20 5231.0 21 5881.5 22 6842.5 23 8091.0 24 9201.5 25 10637.0 26 12453.0 27 14018.0 28 15492.0 29 17157.0 30 18980.0 31 20865.5 32 22883.5 33 25107.0 34 27133.5 35 28456.5 36 29915.0 37 31484.5 38 32725.0 39 33718.5 40 34487.0 41 35433.5 42 36089.0 43 36282.0 44 36723.0 45 37217.5 46 37330.5 47 38163.0 48 38790.0 49 43090.0 50 59277.5 51 57689.5 52 38098.0 53 30110.0 54 27827.0 55 26202.0 56 23998.0 57 21652.5 58 19313.5 59 16524.5 60 13380.0 61 10998.5 62 8889.5 63 6791.0 64 5253.0 65 4169.5 66 3290.0 67 2529.5 68 1928.5 69 1419.5 70 1111.0 71 904.0 72 716.0 73 524.5 74 381.0 75 279.5 76 186.5 77 129.5 78 89.5 79 55.0 80 35.0 81 23.0 82 15.5 83 10.0 84 7.0 85 4.5 86 3.5 87 1.5 88 0.0 89 0.0 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05713221242205652 2 0.0658642099538119 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.6632376250962598E-4 18-19 0.0 20-21 2.910665843918455E-4 22-23 1.247428218822195E-4 24-25 0.0 26-27 0.0020374660907429183 28-29 8.316188125481299E-4 30-31 0.005363941340935438 32-33 9.14780693802943E-4 34-35 0.001829561387605886 36-37 0.006985598025404291 38-39 0.012432701247594543 40-41 0.002827503962663642 42-43 0.0070687599066591045 44-45 0.001496913862586634 46-47 0.0020374660907429183 48-49 0.0031601514876828936 50-51 0.008440930947363518 52-53 0.002162208912625138 54-55 0.0012890091594496013 56-57 6.652950500385039E-4 58-59 5.82133168783691E-4 60-61 0.006736112381639852 62-63 0.001496913862586634 64-65 0.007900378719207233 66-67 0.00985468292869534 68-69 0.01093578738500791 70-71 0.009563616344303494 72-73 0.004199675003368057 74-75 0.0019543042094881053 76-77 0.004449160647132496 78-79 0.015883919319669284 80-81 0.008357769066108705 82-83 0.0054886841628176575 84-85 0.004615484409642121 86-87 0.0070687599066591045 88-89 0.0034927990127021453 90-91 0.005696588865954691 92-93 0.010187330453714592 94-95 0.00748456931293317 96-97 0.009979425750577559 98-99 0.0018711423282332924 100 0.0015800757438414468 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1202474.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.52282423914016 #Duplication Level Percentage of deduplicated Percentage of total 1 93.34652208951275 70.49792981101287 2 4.844184938978433 7.316930554567162 3 0.6997841327321038 1.5854902218499742 4 0.2645747371558459 0.7992572548935062 5 0.154737394914954 0.5843102539692246 6 0.11235717881500656 0.5091318880590832 7 0.08967930165360956 0.4740983895672071 8 0.07399487005654358 0.4470641252702685 9 0.07220215817617122 0.49076198114630193 >10 0.31897197919280273 3.5525668073847614 >50 0.009469009955420033 0.4887502906352835 >100 0.007805510895655587 1.4190185890016893 >500 0.004507396469046866 2.5527527262132073 >1k 8.794919939603639E-4 0.8960289930817971 >5k 0.0 0.0 >10k+ 3.298094977351365E-4 8.385908113347636 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 65092 5.413173174638287 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 21859 1.8178355623489573 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG 14051 1.1685075935113773 No Hit TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2569 0.21364287294361456 No Hit GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT 1468 0.12208164168206548 No Hit ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 1280 0.10644720800616063 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.001164266337567382 8.316188125481299E-5 0.0 0.0 0.0 2 0.001164266337567382 1.6632376250962598E-4 0.0 0.0 0.0 3 0.001164266337567382 1.6632376250962598E-4 0.0 0.0 0.0 4 0.001164266337567382 1.6632376250962598E-4 0.0 0.0 0.0 5 0.001164266337567382 2.49485643764439E-4 0.0 0.0 0.0 6 0.001164266337567382 3.3264752501925197E-4 0.0 0.0 0.0 7 0.001164266337567382 3.3264752501925197E-4 0.0 0.0 0.0 8 0.001164266337567382 3.3264752501925197E-4 0.0 0.0 0.0 9 0.001164266337567382 4.1580940627406496E-4 0.0 0.0 0.0 10-11 0.001164266337567382 4.1580940627406496E-4 0.0 0.0 0.0 12-13 0.001164266337567382 4.1580940627406496E-4 0.0 0.0 0.0 14-15 0.001164266337567382 4.573903469014714E-4 0.0 0.0 0.0 16-17 0.001164266337567382 4.98971287528878E-4 0.0 0.0 0.0 18-19 0.001164266337567382 6.237141094110974E-4 0.0 0.0 0.0 20-21 0.0012474282188221948 6.652950500385039E-4 0.0 0.0 0.0 22-23 0.0012890091594496013 7.900378719207235E-4 0.0 0.0 0.0 24-25 0.0013305901000770079 9.14780693802943E-4 0.0 0.0 0.0 26-27 0.001413751981331821 9.97942575057756E-4 0.0 0.0 0.0 28-29 0.0015800757438414468 9.97942575057756E-4 0.0 0.0 0.0 30-31 0.0017048185657236664 0.001081104456312569 0.0 0.0 0.0 32-33 0.0019543042094881053 0.001164266337567382 0.0 0.0 0.0 34-35 0.0022037898532525444 0.0012890091594496013 0.0 0.0 0.0 36-37 0.0025364373782717966 0.0013721710407044144 0.0 0.0 0.0 38-39 0.0035343799533295523 0.0017048185657236664 0.0 0.0 0.0 40-41 0.004573903469014715 0.0017463995063510729 0.0 0.0 0.0 42-43 0.006195560153483568 0.0018711423282332924 0.0 0.0 0.0 44-45 0.007775635897325014 0.001912723268860699 0.0 0.0 0.0 46-47 0.010145749513087186 0.001912723268860699 0.0 0.0 0.0 48-49 0.013014834416378233 0.001995885150115512 0.0 0.0 0.0 50-51 0.017256090360373698 0.002079047031370325 0.0 0.0 0.0 52-53 0.02207947947315285 0.002079047031370325 0.0 0.0 0.0 54-55 0.029564048786086018 0.0022869517345073575 0.0 0.0 0.0 56-57 0.03929398889289914 0.0024948564376443896 0.0 0.0 0.0 58-59 0.05139404261547443 0.0025780183188992027 0.0 0.0 0.0 60-61 0.06561472431004745 0.002619599259526609 0.0 0.0 0.0 62-63 0.08274607184853894 0.0027443420814088288 0.0 0.0 0.0 64-65 0.10740356964059097 0.0027443420814088288 0.0 0.0 0.0 66-67 0.13555386644534517 0.002910665843918455 0.0 0.0 0.0 68-69 0.1721866751380903 0.0029522467845458614 0.0 0.0 0.0 70-71 0.21933946180956926 0.003368056190819926 0.0 0.0 0.0 72-73 0.2763053504691162 0.003575960893956959 0.0 0.0 0.0 74-75 0.3509431388953108 0.003575960893956959 0.0 0.0 0.0 76-77 0.438928409262903 0.003659122775211772 0.0 0.0 0.0 78-79 0.5513632727194102 0.0037007037158391784 0.0 0.0 0.0 80-81 0.6862102631740894 0.0038670274783488045 0.0 0.0 0.0 82-83 0.8504133977117176 0.0039086084189762105 0.0 0.0 0.0 84-85 1.0566964441642812 0.0039501893596036175 0.0 0.0 0.0 86-87 1.3060989260474656 0.004074932181485837 0.0 0.0 0.0 88 1.5210308081505297 0.00415809406274065 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGAGT 795 0.0 84.62613 1 GTGGAAT 29240 0.0 57.87773 6 TGGAATC 29465 0.0 57.67502 7 GAATCAC 29540 0.0 57.528587 9 ACGTGTG 605 0.0 57.516838 2 GGAATCA 29680 0.0 57.30473 8 AGTGGAA 29175 0.0 56.5247 5 AACGAGT 27445 0.0 55.884315 1 ACGAGTG 28850 0.0 55.71151 2 AACGTGT 550 0.0 55.601463 1 CGAGTGG 29480 0.0 55.397877 3 CGTGTGG 680 0.0 55.29003 3 ATCGAGT 335 0.0 54.771595 1 GAGTGGA 30370 0.0 54.331512 4 TCGAGTG 380 0.0 53.211258 2 CGATTGG 170 0.0 44.232025 3 GAGTACT 9780 0.0 41.32619 20-21 AGTACTT 10095 0.0 40.47893 20-21 GTACTTT 9920 0.0 39.890198 22-23 TACTTTT 10670 0.0 37.680912 22-23 >>END_MODULE