FastQCFastQC Report
Thu 26 May 2016
SRR2132686_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132686_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2581353
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1730976.705669468685607No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA955853.70290308996871No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG126010.4881548552251474No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT68560.2655971500217134No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA42430.16437116504406799No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40790.15801790766315185No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36260.14046897111708473No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT30620.11861996402661706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT18000.083.130011
AACGAGT469500.073.323191
ACGAGTG497700.072.428532
GTGGAAT523250.071.938696
CGAGTGG508650.071.546033
TGGAATC529550.071.464437
GAATCAC530550.071.3297359
GGAATCA534450.070.879578
AGTGGAA524750.070.1658555
GAGTGGA527350.069.7931754
AACGTGT9600.058.328251
CGTGTGG11950.058.2013473
ATCGAGT6400.055.8774831
TCGAGTG7400.053.3772352
ACGTGTG12050.051.1202242
CGATTGG3750.042.6075823
GAGTACT265900.041.709320-21
AGTACTT271950.041.49853520-21
GTACTTT267500.040.6253622-23
TACTTTT292000.038.1018822-23