##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132686_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2581353
Sequences flagged as poor quality	0
Sequence length	100
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.30784437463609	31.0	30.0	34.0	16.0	34.0
2	30.425048801926742	33.0	30.0	34.0	23.0	34.0
3	30.873608917494042	33.0	31.0	34.0	25.0	34.0
4	35.27876388854992	37.0	35.0	37.0	32.0	37.0
5	34.760850608188804	37.0	35.0	37.0	32.0	37.0
6	35.27958245152833	37.0	35.0	37.0	32.0	37.0
7	35.66854630110644	37.0	35.0	37.0	35.0	37.0
8	35.84659401484415	37.0	35.0	37.0	35.0	37.0
9	37.75612324234616	39.0	39.0	39.0	35.0	39.0
10-11	37.83448892886793	39.0	39.0	39.0	35.0	39.0
12-13	37.818749702191056	39.0	39.0	39.0	35.0	39.0
14-15	39.24043999406513	41.0	40.0	41.0	37.0	41.0
16-17	39.15893448125847	41.0	39.5	41.0	36.5	41.0
18-19	39.00352373348395	41.0	39.0	41.0	35.5	41.0
20-21	38.84318998602671	41.0	39.0	41.0	35.0	41.0
22-23	38.754363699966646	40.5	39.0	41.0	35.0	41.0
24-25	38.68880118294554	41.0	39.0	41.0	35.0	41.0
26-27	38.63404423959064	41.0	39.0	41.0	35.0	41.0
28-29	38.478247453951475	41.0	39.0	41.0	35.0	41.0
30-31	38.36668793458314	40.5	38.0	41.0	35.0	41.0
32-33	38.25126493741848	40.0	38.0	41.0	35.0	41.0
34-35	38.1809041614998	40.0	38.0	41.0	34.5	41.0
36-37	38.041479603913146	40.0	38.0	41.0	34.0	41.0
38-39	37.62950030468518	40.0	36.0	41.0	34.0	41.0
40-41	37.44141483167935	40.0	35.0	41.0	33.0	41.0
42-43	37.312079363031714	40.0	35.0	41.0	33.0	41.0
44-45	37.18057448942473	40.0	35.0	41.0	33.0	41.0
46-47	37.055116832141906	40.0	35.0	41.0	33.0	41.0
48-49	36.91769471281146	40.0	35.0	41.0	33.0	41.0
50-51	36.68645357686454	40.0	35.0	41.0	32.5	41.0
52-53	36.253000073992204	39.5	35.0	41.0	31.0	41.0
54-55	35.52553486485576	39.0	35.0	41.0	28.0	41.0
56-57	34.932333934955814	39.0	35.0	41.0	23.5	41.0
58-59	34.62707212070569	39.0	35.0	41.0	22.0	41.0
60-61	34.39011208463158	38.5	35.0	41.0	21.0	41.0
62-63	34.09909396351448	38.0	35.0	40.0	19.5	41.0
64-65	33.84469113677982	37.5	35.0	40.0	18.0	41.0
66-67	33.54922224895239	37.0	34.0	40.0	17.5	41.0
68-69	33.25934732677011	36.5	34.0	39.5	14.0	41.0
70-71	32.92191749830418	36.0	34.0	39.0	11.5	41.0
72-73	32.57625129147389	35.5	34.0	39.0	7.5	40.5
74-75	32.2330496836349	35.0	33.5	38.5	7.0	40.0
76-77	31.866994750427395	35.0	33.0	37.0	2.0	39.5
78-79	31.73301598037928	35.0	33.5	37.0	2.0	39.0
80-81	31.58521054656221	35.0	34.0	36.5	2.0	39.0
82-83	31.274406483731592	35.0	33.5	36.0	2.0	38.5
84-85	30.997143745934785	35.0	33.0	36.0	2.0	37.0
86-87	30.796713971316592	35.0	33.0	35.5	2.0	37.0
88-89	30.584122163841986	35.0	33.0	35.0	2.0	36.5
90-91	30.3882001028143	35.0	33.0	35.0	2.0	36.0
92-93	30.224741637428124	35.0	33.0	35.0	2.0	36.0
94-95	30.05785841765927	35.0	33.0	35.0	2.0	36.0
96-97	29.890029569764383	35.0	33.0	35.0	2.0	36.0
98-99	29.751112691677584	35.0	33.0	35.0	2.0	35.0
100	29.646990938472964	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	2.8318579390286587
1101	2	2.245274310676038
1101	3	2.0583920114523373
1101	4	1.076433799473513
1101	5	1.6210307624073295
1101	6	1.0652664412835193
1101	7	0.5721883793778773
1101	8	0.45467132884129313
1101	9	0.4392718134279221
1101	10-11	0.3801157302932552
1101	12-13	0.3303251125137976
1101	14-15	0.5010472325288546
1101	16-17	0.4803278113073688
1101	18-19	0.5052758662812948
1101	20-21	0.53520382206721
1101	22-23	0.5229540537562158
1101	24-25	0.5725736810804918
1101	26-27	0.5343893758711431
1101	28-29	0.5460132105319815
1101	30-31	0.583891608511685
1101	32-33	0.5251443308426147
1101	34-35	0.5226580949045427
1101	36-37	0.5797919430226273
1101	38-39	0.5125722934808579
1101	40-41	0.461153807367495
1101	42-43	0.4444797357698249
1101	44-45	0.4962853782611063
1101	46-47	0.4852014443424082
1101	48-49	0.3715918133424694
1101	50-51	0.44242089005223306
1101	52-53	0.3769692496175949
1101	54-55	0.33663667897491933
1101	56-57	0.26197315708007096
1101	58-59	0.17833454927230008
1101	60-61	0.19166366878756236
1101	62-63	0.17927281239676063
1101	64-65	0.16363021181853554
1101	66-67	0.14927833718295602
1101	68-69	0.18579106202739837
1101	70-71	0.19362268241552272
1101	72-73	0.2158617295375862
1101	74-75	0.19833371565430724
1101	76-77	0.11847937607930703
1101	78-79	0.17753833222120008
1101	80-81	0.15600212749803433
1101	82-83	0.25073620718263356
1101	84-85	0.2681909895294332
1101	86-87	0.2586235761354523
1101	88-89	0.2392782481264959
1101	90-91	0.1069897864451157
1101	92-93	0.1964886273978088
1101	94-95	0.30218350844815234
1101	96-97	0.3132895380500713
1101	98-99	0.24565928489836963
1101	100	0.2502287336131346
1102	1	2.7219959053035723
1102	2	2.237865010727134
1102	3	2.0397410150292465
1102	4	1.070301965033046
1102	5	1.5356960664441175
1102	6	0.9855525935574079
1102	7	0.4531285939920764
1102	8	0.4015287636905569
1102	9	0.3853627689721222
1102	10-11	0.34319697709959485
1102	12-13	0.2909791748541082
1102	14-15	0.4385791691665588
1102	16-17	0.407767770428471
1102	18-19	0.45443273904572834
1102	20-21	0.5211517014744729
1102	22-23	0.48833473847773234
1102	24-25	0.5246687245143207
1102	26-27	0.494149212355552
1102	28-29	0.5130546004144492
1102	30-31	0.5624301879700369
1102	32-33	0.5818637994169578
1102	34-35	0.5404148654717389
1102	36-37	0.5759595464973373
1102	38-39	0.45534183870313427
1102	40-41	0.4817210020532414
1102	42-43	0.49762230092056114
1102	44-45	0.5095710195487868
1102	46-47	0.4605463600296815
1102	48-49	0.46088665238180937
1102	50-51	0.49530796210128614
1102	52-53	0.46984099208054886
1102	54-55	0.5435860913407851
1102	56-57	0.42612747491348557
1102	58-59	0.34453281191920837
1102	60-61	0.2833856922929243
1102	62-63	0.22641128965787516
1102	64-65	0.27643041621302444
1102	66-67	0.30576786196579064
1102	68-69	0.34636847643721325
1102	70-71	0.3427029072494534
1102	72-73	0.33709320628771167
1102	74-75	0.3324675940395849
1102	76-77	0.2528687475662821
1102	78-79	0.2682383833198152
1102	80-81	0.35439252095741125
1102	82-83	0.4318808162781913
1102	84-85	0.42174234364287244
1102	86-87	0.4022106992831276
1102	88-89	0.3676635317238386
1102	90-91	0.3364225917593764
1102	92-93	0.38721422780659864
1102	94-95	0.3983766612330264
1102	96-97	0.3919814133694359
1102	98-99	0.4319137560276509
1102	100	0.4114448807771609
1103	1	2.587872637419345
1103	2	2.1932338789735724
1103	3	2.0210715796256515
1103	4	1.0069135377512737
1103	5	1.512508369138942
1103	6	0.9535634209267556
1103	7	0.395736976054998
1103	8	0.3234836760484612
1103	9	0.35739737007486383
1103	10-11	0.23205104039744384
1103	12-13	0.20107258220043178
1103	14-15	0.3594508075948468
1103	16-17	0.3849820752472084
1103	18-19	0.3198187031385018
1103	20-21	0.40534775480802665
1103	22-23	0.3769029005306166
1103	24-25	0.47546265525596
1103	26-27	0.453208205835665
1103	28-29	0.48941188082915943
1103	30-31	0.45919085375569324
1103	32-33	0.4514567624808947
1103	34-35	0.4553576923395397
1103	36-37	0.5092877190727378
1103	38-39	0.5144156092469103
1103	40-41	0.45502805455568307
1103	42-43	0.4867543011177631
1103	44-45	0.39547898665054504
1103	46-47	0.5002004227581835
1103	48-49	0.5762929317022198
1103	50-51	0.6388613774986496
1103	52-53	0.6571165298420922
1103	54-55	0.6180368070514319
1103	56-57	0.39442394473508813
1103	58-59	0.33780019020543506
1103	60-61	0.3351935340484289
1103	62-63	0.30015524248099723
1103	64-65	0.3132747665029214
1103	66-67	0.3902194925086562
1103	68-69	0.4681318045528542
1103	70-71	0.4053578512965288
1103	72-73	0.4073621229672
1103	74-75	0.4352753914121408
1103	76-77	0.3514071122266884
1103	78-79	0.32823678786277455
1103	80-81	0.3166544947461105
1103	82-83	0.347452185937307
1103	84-85	0.34697877989647097
1103	86-87	0.2987755883846326
1103	88-89	0.2510932674977795
1103	90-91	0.2638923120419818
1103	92-93	0.342870679450602
1103	94-95	0.43471575110823935
1103	96-97	0.47217328357764643
1103	98-99	0.383101698668316
1103	100	0.40053528004643724
1104	1	1.5990122395577657
1104	2	1.7797380143691868
1104	3	1.7960798650456304
1104	4	0.8466982959078067
1104	5	1.450316455607954
1104	6	0.9712940935767449
1104	7	0.4074098338766916
1104	8	0.2568830237072106
1104	9	0.2446939674281694
1104	10-11	0.1674651827644098
1104	12-13	0.17162661574970173
1104	14-15	0.3553069310877106
1104	16-17	0.26912923565596003
1104	18-19	0.3299466517139109
1104	20-21	0.37751839107783525
1104	22-23	0.3268804025256813
1104	24-25	0.3730707856616604
1104	26-27	0.3072293521947671
1104	28-29	0.3750691126262069
1104	30-31	0.3371271334533006
1104	32-33	0.3367322281038483
1104	34-35	0.3165506137407945
1104	36-37	0.3999046322758346
1104	38-39	0.35960903155665846
1104	40-41	0.37029267346672157
1104	42-43	0.41436526773496496
1104	44-45	0.41740722709469225
1104	46-47	0.4759282407846399
1104	48-49	0.40145237874565254
1104	50-51	0.3793221099045496
1104	52-53	0.30083993957111943
1104	54-55	0.24196471532212627
1104	56-57	0.23077233441264156
1104	58-59	0.3034764191967909
1104	60-61	0.29963921422970685
1104	62-63	0.34098098736169646
1104	64-65	0.26294386675423453
1104	66-67	0.28734831073789735
1104	68-69	0.2631515230957504
1104	70-71	0.2091143056008633
1104	72-73	0.21500071269539944
1104	74-75	0.2616599330127727
1104	76-77	0.32152310306203447
1104	78-79	0.23057999795478779
1104	80-81	0.33800947114337987
1104	82-83	0.31968187316459407
1104	84-85	0.3120659495768301
1104	86-87	0.2930618959273126
1104	88-89	0.4114400734872916
1104	90-91	0.30053363608488226
1104	92-93	0.3026703135301325
1104	94-95	0.3278680721860425
1104	96-97	0.33628722537944356
1104	98-99	0.36374074037437154
1104	100	0.36808001626993914
1105	1	2.100061399936589
1105	2	1.9545627902472944
1105	3	1.908987521359851
1105	4	0.9651765392685974
1105	5	1.5276945809404907
1105	6	1.0201707506338877
1105	7	0.5478687041051629
1105	8	0.3691528856951791
1105	9	0.30813586267492354
1105	10-11	0.26681264710943964
1105	12-13	0.21969854025457636
1105	14-15	0.35330028601887875
1105	16-17	0.3117249334821537
1105	18-19	0.29591999187447016
1105	20-21	0.3578042435024926
1105	22-23	0.36354457650850236
1105	24-25	0.4482409149948552
1105	26-27	0.3257038187002266
1105	28-29	0.37559549277090554
1105	30-31	0.43167732035578155
1105	32-33	0.4198291164631769
1105	34-35	0.4365764525565723
1105	36-37	0.42646931815374955
1105	38-39	0.4316757023886453
1105	40-41	0.3949142313420566
1105	42-43	0.4452558563240032
1105	44-45	0.49514699090150316
1105	46-47	0.4640866705304916
1105	48-49	0.35922374976731675
1105	50-51	0.3635653013143596
1105	52-53	0.4041605458985629
1105	54-55	0.3763440382655858
1105	56-57	0.3611438280997419
1105	58-59	0.3126145176239632
1105	60-61	0.36591372046841286
1105	62-63	0.44110224943420917
1105	64-65	0.39157532230913006
1105	66-67	0.31452562207573465
1105	68-69	0.228735157682614
1105	70-71	0.3214046992821977
1105	72-73	0.314956867004895
1105	74-75	0.40485177603030564
1105	76-77	0.3929140889835736
1105	78-79	0.36296256032964536
1105	80-81	0.422953540736124
1105	82-83	0.39111877319983535
1105	84-85	0.4509417743834554
1105	86-87	0.3512799243282885
1105	88-89	0.32633187237846784
1105	90-91	0.3783451845492003
1105	92-93	0.4261170436398345
1105	94-95	0.4508288753834506
1105	96-97	0.49563720600094285
1105	98-99	0.38058374571151177
1105	100	0.41459049481642296
1106	1	1.7052810173025463
1106	2	1.214008435106372
1106	3	1.5873265433855188
1106	4	0.8305843875528467
1106	5	1.5161906644839718
1106	6	1.0146294645806293
1106	7	0.5897043730481712
1106	8	0.39981661364561205
1106	9	0.3266439988610088
1106	10-11	0.2380798682624956
1106	12-13	0.18363256099100767
1106	14-15	0.25628638693325456
1106	16-17	0.2559437153943165
1106	18-19	0.27773656659456947
1106	20-21	0.33025463873001826
1106	22-23	0.2944288920140039
1106	24-25	0.32608195010360674
1106	26-27	0.333449616346293
1106	28-29	0.2972885118926456
1106	30-31	0.35308580602343653
1106	32-33	0.33525495712965636
1106	34-35	0.31013074626009285
1106	36-37	0.28830516856244515
1106	38-39	0.2776281458063323
1106	40-41	0.3256811871756753
1106	42-43	0.3668411820833839
1106	44-45	0.2802197716888202
1106	46-47	0.28837424572904524
1106	48-49	0.261351239257138
1106	50-51	0.18733569930300575
1106	52-53	0.1270539605443446
1106	54-55	0.23999931940337405
1106	56-57	0.28770264800820655
1106	58-59	0.26752414889754306
1106	60-61	0.2941998150018392
1106	62-63	0.2827302053975842
1106	64-65	0.16621148360533056
1106	66-67	0.2097499433169716
1106	68-69	0.21334301270363198
1106	70-71	0.21239944674007916
1106	72-73	0.1524973380044301
1106	74-75	0.2009254990159377
1106	76-77	0.037448366191505045
1106	78-79	0.06620579100716739
1106	80-81	0.04580112640037726
1106	82-83	0.10222317456113572
1106	84-85	-0.006940454622444747
1106	86-87	0.08401871241740011
1106	88-89	0.08934281392919985
1106	90-91	0.07245141029298807
1106	92-93	0.1420540660322267
1106	94-95	0.17632347612691035
1106	96-97	0.15503450152172604
1106	98-99	0.15423486262688968
1106	100	0.17167251944214712
1107	1	1.6190397582487925
1107	2	1.0936947723711903
1107	3	1.4147821695303158
1107	4	0.7269254436821129
1107	5	1.401531275628166
1107	6	0.9405942496095605
1107	7	0.48835572959411166
1107	8	0.40096364969693354
1107	9	0.4007664704191214
1107	10-11	0.15815330491140145
1107	12-13	0.1481372399196843
1107	14-15	0.21153810454741517
1107	16-17	0.24658421167026745
1107	18-19	0.2921437815618688
1107	20-21	0.2864746457481786
1107	22-23	0.27470184755217986
1107	24-25	0.3319533070822942
1107	26-27	0.29648116418877635
1107	28-29	0.35687488274301415
1107	30-31	0.15200876339611824
1107	32-33	0.1482532722073131
1107	34-35	0.11302046804564014
1107	36-37	0.20087289237442008
1107	38-39	0.1961583638678377
1107	40-41	0.1947509143612507
1107	42-43	0.21357827963294795
1107	44-45	0.30223042216668716
1107	46-47	0.31016781310759
1107	48-49	0.2729257902502624
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2216	3	-5.832256115159318
2216	4	-3.030407664616831
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2216	92-93	-1.1024287290301586
2216	94-95	-1.190293559863484
2216	96-97	-1.06846359209705
2216	98-99	-1.0808459583786636
2216	100	-1.2227609981619025
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4118.0
3	4383.0
4	4822.0
5	6266.0
6	5219.0
7	3729.0
8	3270.0
9	3211.0
10	3509.0
11	6110.0
12	4179.0
13	5271.0
14	5003.0
15	5361.0
16	6258.0
17	7974.0
18	11327.0
19	18709.0
20	39924.0
21	53559.0
22	25772.0
23	16031.0
24	15101.0
25	16499.0
26	17103.0
27	18717.0
28	21615.0
29	25153.0
30	29934.0
31	37382.0
32	49027.0
33	67426.0
34	98300.0
35	225352.0
36	277126.0
37	483566.0
38	686762.0
39	266323.0
40	1962.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.405807303396484	42.16880780033693	26.800478799404292	16.6249060968623
2	17.328685132320995	40.24300327643125	25.293103368072085	17.135208223175674
3	18.545197034268462	26.71013999247681	21.647058732377943	33.097604240876784
4	31.084783832354585	27.63821918195613	23.20856543060945	18.068431555079837
5	17.211012984276074	39.08965569606326	24.354127467262323	19.345203852398335
6	33.718325234867145	25.161494766504234	24.487042260396002	16.633137738232627
7	18.37679697429991	25.20631622253911	38.35058591366621	18.066300889494773
8	32.80616792821439	25.482024349246306	24.04696296864474	17.664844753894567
9	32.59589060465578	26.976628148106823	23.799147191414736	16.628334055822663
10-11	16.735971407242637	42.255650428283154	23.908121051247157	17.100257113227055
12-13	16.678869569562938	26.692358619685102	31.44806231460788	25.180709496144075
14-15	16.924457832772195	34.25848770005497	23.783845138576552	25.033209328596286
16-17	24.97829141052662	26.504023183204612	31.275051073052236	17.242634333216532
18-19	17.257325906220498	26.64339205060292	31.352995890139784	24.7462861530368
20-21	25.104320636009646	34.487163569217515	23.516853329727976	16.891662465044863
22-23	24.81917895303253	26.778870207668426	31.287149885302284	17.114800953996756
24-25	17.161717130512564	34.37588737379196	23.532194163293436	24.93020133240204
26-27	17.472674068252992	29.733523134815936	35.7133351941575	17.08046760277357
28-29	23.8506229145949	26.694715482272148	32.37501709388134	17.079644509251615
30-31	22.012525986035918	27.765619321910712	32.67720287587837	17.544651816174994
32-33	19.231516463976245	28.78028371170923	33.467405694960036	18.52079412935449
34-35	19.182440738033225	28.608627536664766	33.62111546422665	18.58781626107536
36-37	19.047822906706628	28.678537611946314	33.937980309536684	18.335659171810374
38-39	19.494867095420904	28.43747112359788	33.650517707276144	18.41714407370507
40-41	18.750788127807358	29.35459620641036	33.54423036208262	18.350385303699664
42-43	18.855269598845936	28.68497472338294	33.779641208992686	18.680114468778434
44-45	18.833995593711876	29.05298896568449	33.38727878973126	18.725736650872367
46-47	19.214548221307048	28.66514688480553	33.620689488188376	18.499615405699046
48-49	18.801481281752682	28.903667098235836	33.560507624517776	18.734343995493706
50-51	19.294568432634733	29.319867576609333	32.97911948905802	18.406444501697912
52-53	19.3397086201189	29.116564543144367	32.799635993064655	18.744090843672076
54-55	19.508296556169885	29.944539830907697	31.484570636947414	19.062592975975008
56-57	19.773187481380788	30.43984149311656	30.540118907280288	19.246852118222364
58-59	20.53292346670463	30.71278247335915	29.628166127699796	19.126127932236418
60-61	20.164202982687673	31.07926152823372	29.407264966950393	19.349270522128215
62-63	19.69161800115949	31.17527659873673	29.377237113769496	19.75586828633429
64-65	19.984282236331318	31.153928704812884	29.584922414608027	19.276866644247768
66-67	19.899549353300774	30.6748542318811	29.965148154727395	19.460448260090736
68-69	20.20673445457365	30.715965131116697	29.78459656096059	19.292703853349067
70-71	19.431719474859964	31.29986726599724	29.82435409371656	19.444059165426243
72-73	19.323136047852923	30.79592483077433	30.119196472241565	19.761742649131183
74-75	19.542402445272405	31.032393467264168	29.646777844173933	19.778426243289495
76-77	20.00597387533661	30.65121052331845	29.842761557531094	19.500054043813844
78-79	19.757665342211254	31.058124284416305	29.331230736147944	19.852979637224493
80-81	20.02208345803707	31.399403552918454	29.076185024509734	19.50232796453474
82-83	20.067992553749953	31.333603237297886	29.102130239639852	19.496273969312305
84-85	19.920285694582944	31.644440725270005	28.713789381602016	19.721484198545028
86-87	19.905371819979813	31.826159219419214	28.778647543444695	19.48982141715627
88-89	20.64577302254046	31.41808369790189	28.446729730488844	19.489413549068814
90-91	20.308661561307122	31.897920703050392	28.332517824011926	19.46089991163056
92-93	19.86142296776383	32.04285861233513	28.213606530415525	19.882111889485515
94-95	20.100130465987316	32.13165151343451	28.129435431927654	19.638782588650525
96-97	20.0197709200103	31.700576138479352	28.452399563203308	19.82725337830704
98-99	20.495602380676587	31.85301650380838	27.850665734830315	19.80071538068472
100	19.974059411540697	32.43600178670839	27.837552101028827	19.752386700722084
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	97249.0
1	52529.0
2	5660.0
3	2924.5
4	2226.0
5	1997.5
6	2034.5
7	2377.0
8	2213.5
9	1912.0
10	1927.0
11	2875.5
12	24844.5
13	27488.5
14	8147.0
15	7520.5
16	8269.0
17	9231.5
18	10412.5
19	11507.0
20	12730.0
21	14351.5
22	16181.0
23	18649.0
24	21301.5
25	24147.5
26	28672.0
27	33601.5
28	36812.5
29	39680.0
30	42830.0
31	46128.0
32	50130.5
33	54125.0
34	58870.5
35	63024.5
36	66148.0
37	69499.5
38	72374.0
39	73919.0
40	75259.5
41	77694.5
42	79003.5
43	79164.5
44	79926.0
45	80299.0
46	79672.5
47	80332.5
48	80382.5
49	82121.0
50	94440.5
51	90266.5
52	69988.0
53	61633.5
54	57788.0
55	53899.0
56	48551.0
57	42726.5
58	39262.0
59	33300.5
60	25924.5
61	21289.5
62	16884.5
63	12810.5
64	9594.0
65	7233.0
66	5551.5
67	4381.0
68	3619.0
69	2874.0
70	2108.0
71	1478.5
72	1090.0
73	813.5
74	633.5
75	478.0
76	350.0
77	245.0
78	156.0
79	97.5
80	52.0
81	30.0
82	22.5
83	14.5
84	4.0
85	3.0
86	3.5
87	2.0
88	1.0
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.060704599487168165
2	0.06651550562824998
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	5.810906141081828E-5
18-19	0.0
20-21	3.292846813279702E-4
22-23	9.684843568469712E-5
24-25	0.0
26-27	0.0023049927692957916
28-29	7.74787485477577E-4
30-31	0.006159560509546738
32-33	0.0013752477867226994
34-35	0.0023243624564327314
36-37	0.007941571726145165
38-39	0.011989836337765505
40-41	0.003002301506225611
42-43	0.008232117033199256
44-45	0.001627053719502912
46-47	0.0023824715178435494
48-49	0.002905453070540914
50-51	0.009219971077183167
52-53	0.0032347377518688845
54-55	0.001588314345229033
56-57	5.423512398343039E-4
58-59	9.878540439839107E-4
60-61	0.00610145144813592
62-63	0.0016076840323659724
64-65	0.007631656731954134
66-67	0.011021351980918534
68-69	0.012106054460587143
70-71	0.009723582942743593
72-73	0.004280700857263613
74-75	0.001762641529461488
76-77	0.004416288667222189
78-79	0.016193058446481362
80-81	0.00912312264149847
82-83	0.007283002363489224
84-85	0.005229815526973645
86-87	0.007670396106228014
88-89	0.004087003985894219
90-91	0.005578469895438555
92-93	0.010130346372619321
94-95	0.008542032027390288
96-97	0.011215048852287928
98-99	0.0027117561991715196
100	0.0027117561991715196
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2581353.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.61145648860382
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.74730535655503	60.00334574595556
2	8.506108918019626	11.502208259960176
3	1.3608072037301129	2.760184711331314
4	0.3994222117924386	1.0802206997274544
5	0.20343936664105597	0.6877415942860438
6	0.1296626190883103	0.5260007117212622
7	0.09459908010199691	0.44771871117246775
8	0.0624242201084535	0.33764739533581845
9	0.04631808470268151	0.2818469851659735
>10	0.41759751117312033	6.375510720950893
>50	0.018851024495520965	0.8240633007787743
>100	0.009784794549991285	1.4702702669202563
>500	0.0027022128899668123	1.3488353952835366
>1k	7.474205865861325E-4	1.153734532176678
>5k	5.749389127585634E-5	0.26650956713623
>10k+	1.7248167382756906E-4	10.934161402097457
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	173097	6.705669468685607	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	95585	3.70290308996871	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	12601	0.4881548552251474	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6856	0.2655971500217134	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA	4243	0.16437116504406799	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4079	0.15801790766315185	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3626	0.14046897111708473	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3062	0.11861996402661706	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0019757080879678215	0.0	0.0	3.873937427387885E-5	0.0
2	0.0019757080879678215	0.0	0.0	3.873937427387885E-5	0.0
3	0.0019757080879678215	0.0	0.0	7.74787485477577E-5	0.0
4	0.0019757080879678215	0.0	0.0	1.1621812282163656E-4	0.0
5	0.0019757080879678215	0.0	0.0	1.549574970955154E-4	0.0
6	0.0019757080879678215	0.0	0.0	1.549574970955154E-4	0.0
7	0.0019757080879678215	0.0	0.0	1.549574970955154E-4	0.0
8	0.0019757080879678215	0.0	0.0	2.7117561991715197E-4	0.0
9	0.0019757080879678215	0.0	0.0	2.7117561991715197E-4	0.0
10-11	0.0019757080879678215	0.0	0.0	3.099149941910308E-4	0.0
12-13	0.0019757080879678215	0.0	0.0	3.099149941910308E-4	0.0
14-15	0.0020144474622417006	0.0	0.0	3.292846813279703E-4	0.0
16-17	0.0020725565236525186	0.0	0.0	3.486543684649097E-4	0.0
18-19	0.0021500352722002764	0.0	0.0	3.486543684649097E-4	0.0
20-21	0.0022081443336110945	0.0	0.0	4.06763429875728E-4	0.0
22-23	0.002324362456432731	0.0	0.0	4.261331170126674E-4	0.0
24-25	0.0024793199535282466	0.0	0.0	4.842421784234857E-4	0.0
26-27	0.0025180593278021253	0.0	0.0	5.036118655604251E-4	0.0
28-29	0.0025374290149390646	0.0	0.0	5.036118655604251E-4	0.0
30-31	0.0025567987020760044	0.0	0.0	5.229815526973645E-4	0.0
32-33	0.0025567987020760044	0.0	0.0	5.810906141081827E-4	0.0
34-35	0.0028086046348562168	0.0	0.0	6.585693626559406E-4	0.0
36-37	0.0032734771261427632	0.0	0.0	6.779390497928799E-4	0.0
38-39	0.0036221314946076727	0.0	0.0	7.166784240667588E-4	0.0
40-41	0.004435658354359129	0.0	0.0	7.747874854775771E-4	0.0
42-43	0.005462251772616918	0.0	0.0	8.13526859751456E-4	0.0
44-45	0.006353257380916132	0.0	0.0	8.522662340253349E-4	0.0
46-47	0.007050566117845951	0.0	0.0	9.491146697100319E-4	0.0
48-49	0.008077159536103742	0.0	0.0	0.001045963105394729	0.0
50-51	0.009394298261415622	0.0	0.0	0.0010653327925316683	0.0
52-53	0.011001982293781593	0.0	0.0	0.0011428115410794261	0.0
54-55	0.013365084124488204	0.0	0.0	0.001181550915353305	0.0
56-57	0.016386755317850756	0.0	0.0	0.0012590296639010626	0.0
58-59	0.01966023244399352	0.0	0.0	0.0013171387253118811	0.0
60-61	0.0237278667427508	0.0	0.0	0.0014333568481335176	0.0
62-63	0.028395961342753198	0.0	0.0	0.0015495749709551541	0.0
64-65	0.03492354590790178	0.0	0.0	0.001627053719502912	0.0
66-67	0.04404666854940026	0.0	0.0	0.0016657930937767906	0.0
68-69	0.05553289302160534	0.0	0.0	0.0016657930937767906	0.0
70-71	0.07124170928966321	0.0	0.0	0.0017432718423245484	0.0
72-73	0.09117311735357388	0.0	0.0	0.0017820112165984274	0.0
74-75	0.11714786780420966	0.0	0.0	0.0018982293394200639	0.0
76-77	0.14937902720007684	0.0	0.0	0.0019175990265570034	0.0
78-79	0.18935806145071984	0.0	0.0	0.0020531868365155793	0.0
80-81	0.24111386548062197	0.0	0.0	0.002091926210789458	0.0
82-83	0.30530500865243926	0.0	0.0	0.0021500352722002764	0.0
84-85	0.3869869793089128	0.0	0.0	0.0022081443336110945	0.0
86-87	0.49191257452971365	0.0	0.0	0.0022081443336110945	0.0
88	0.5808194384882657	0.0	0.0	0.0022081443336110945	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	1800	0.0	83.13001	1
AACGAGT	46950	0.0	73.32319	1
ACGAGTG	49770	0.0	72.42853	2
GTGGAAT	52325	0.0	71.93869	6
CGAGTGG	50865	0.0	71.54603	3
TGGAATC	52955	0.0	71.46443	7
GAATCAC	53055	0.0	71.329735	9
GGAATCA	53445	0.0	70.87957	8
AGTGGAA	52475	0.0	70.165855	5
GAGTGGA	52735	0.0	69.793175	4
AACGTGT	960	0.0	58.32825	1
CGTGTGG	1195	0.0	58.201347	3
ATCGAGT	640	0.0	55.877483	1
TCGAGTG	740	0.0	53.377235	2
ACGTGTG	1205	0.0	51.120224	2
CGATTGG	375	0.0	42.607582	3
GAGTACT	26590	0.0	41.7093	20-21
AGTACTT	27195	0.0	41.498535	20-21
GTACTTT	26750	0.0	40.62536	22-23
TACTTTT	29200	0.0	38.10188	22-23
>>END_MODULE